An electrical stimulation apparatus and method for experimental animals

Abstract

An electric acupuncture stimulation experiment device and method for experimental animals, the electric acupuncture stimulation experiment device comprises a base plate, a needle body and an electric connection assembly; clamping arms are installed on the left and right sides of the base plate for clamping and fixing the experimental animals, a plurality of adjusting plates are connected below the base plate, the lower surface of the adjusting plate is a concave surface for fitting the experimental animals, the needle body is installed in the adjusting plate, and the needle tip of the needle body protrudes from the lower surface of the adjusting plate; the electric connection assembly comprises first and second conductive magnets that can be magnetically attracted to each other, the first conductive magnet is fixed on the base plate and electrically connected with the needle body, and the second conductive magnet is used for connecting an external electric pulse generating device. An electric acupuncture stimulation experiment method is also disclosed, which adopts the above device and can be stably fixed on the experimental animals, is not easy to fall off the needle, and has controllable penetration depth and angle, and belongs to the technical field of medical instruments.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and specifically to an electroacupuncture stimulation experimental device and method for laboratory animals. Background Technology

[0002] In acupuncture mechanism and neuroscience research, animal acupuncture experiments are an important means of verifying acupoint specificity, stimulation parameters, and central nervous system response. However, existing animal acupuncture devices generally suffer from the following technical problems:

[0003] 1. Difficulty in fixing the needle: When conventional acupuncture needles are inserted into small parts such as the head of experimental animals (especially mice), the needles are thin and short and are mostly inserted in a straight manner, making it difficult to keep them stable and they are very easy to fall over and fall off.

[0004] 2. Animal activity is restricted: The wire is directly connected to the needle handle, and it is easy to be pulled by the animal's movement, which can cause the needle to shift or fall off. This makes it impossible to conduct continuous stimulation in a free-moving state in behavioral experiments. Moreover, after the needle shifts or falls off, it needs to be repeatedly reinserted, which not only prolongs the experimental time, but also causes additional damage to the acupoint tissue due to repeated acupuncture, introducing non-specific variables and affecting the accuracy of the experiment.

[0005] 3. Large operational error: Relying on manual diagnosis and insertion, the consistency of acupoint location, needling angle and depth is poor, affecting the reliability of experimental results;

[0006] 4. Inconvenient control setup: The sham stimulation (no power applied) control group requires disconnecting the wires or placing a sham needle, which is cumbersome and makes it difficult to achieve a "blind" control.

[0007] To address the aforementioned issues, a new electroacupuncture stimulation experimental device is needed that provides stability and fixation, allows for free movement of experimental animals, prevents needle dislodgement, and facilitates convenient control. Summary of the Invention

[0008] In view of the technical problems existing in the prior art, the purpose of this invention is to provide an electroacupuncture stimulation experimental device for laboratory animals that can be stably fixed, is not easy to dislodge, and has a controllable insertion depth.

[0009] Another object of the present invention is to provide an electroacupuncture stimulation experiment method for laboratory animals.

[0010] To achieve the above objectives, the present invention adopts the following technical solution: an electroacupuncture stimulation experimental device for laboratory animals, comprising a base plate, a needle body, and an electrical connection assembly; clamping arms are installed on the left and right sides of the base plate for clamping and fixing the laboratory animal; multiple adjustment plates are connected to the bottom of the base plate, the lower surface of the adjustment plates being a concave surface for conforming to the laboratory animal; the needle body is installed in the adjustment plate, and the needle tip of the needle body protrudes from the lower surface of the adjustment plate; the electrical connection assembly includes a first conductive magnet and a second conductive magnet that can magnetically attract each other; the first conductive magnet is fixed to the base plate and electrically connected to the needle body; the second conductive magnet is used to connect to an external electrical pulse generator.

[0011] With this structure, the device and the experimental animal are stably connected and the needle is precisely and controllably inserted by the coordination of the adjustment plate, the needle body and the clamping arm. Furthermore, the connection between the first and second conductive magnets enables rapid switching between power on and off, making comparison convenient.

[0012] As a preferred embodiment, the lower surface of the adjusting plate is an arc-shaped concave surface that curves in the left-right direction and extends in a straight line in the front-back direction. The needle body is fixedly connected to the adjusting plate, and the needle tip is tilted backward at 15°~30°.

[0013] As a preferred embodiment, multiple adjustment plates are arranged sequentially in the left-right direction. The number and size of the adjustment plates are determined according to the body size of the experimental animal. Adjacent adjustment plates are hinged to adjust the angle between them. An angle locking mechanism is provided between adjacent adjustment plates to lock them after the angle is adjusted.

[0014] As a preferred embodiment, both the adjustment plate and the base plate are made of rigid plastic, and the first conductive magnet is embedded in the base plate; the lower surface of the first conductive magnet is connected to the needle body through a conductive wire, and the upper surface is exposed to attract the second conductive magnet; the second conductive magnet is provided with a clamping part to be clamped by the electrode clamp of the power pulse generator.

[0015] As a preferred embodiment, the magnetic attraction between the first conductive magnet and the second conductive magnet is configured such that when the second conductive magnet is pulled, the second conductive magnet separates from the first conductive magnet before the needle begins to detach or shift from the surface of the experimental animal, thereby forming a protection against needle detachment.

[0016] As a preferred embodiment, the end of the gripping arm is provided with a pre-inflated airbag or an instant-inflated airbag for contacting the experimental animal; the pre-inflated airbag is a fully sealed airbag filled with air; the instant-inflated airbag is provided with an inflation port for inflating the airbag after the gripping arm has initially gripped the experimental animal.

[0017] As a preferred option, the clamping arm adopts a retractable arc-shaped flexible arm.

[0018] As a preferred option, the number of needles is 1 to 3, which are used for single-acupoint, double-acupoint, or multi-acupoint stimulation experiments.

[0019] An electroacupuncture stimulation experiment method for laboratory animals, employing the aforementioned electroacupuncture stimulation experiment device for laboratory animals, includes the following steps:

[0020] S1. Adjust the angle between adjacent adjustment plates according to the size of the experimental animal so that the enclosed shape of multiple adjustment plates matches the surface of the experimental animal.

[0021] S2, bring the adjustment plate close to the surface of the experimental animal, align the needle with the corresponding acupoint, and insert the needle into the acupoint at the angle of the needle tip;

[0022] S3, the experimental animal is held and fixed by the clamping arm;

[0023] S4, the second conductive magnet is attracted to the first conductive magnet, and the electric pulse generator is connected to the second conductive magnet, so that the electric pulse generator is electrically connected to the needle body;

[0024] S6, turn on the electrical pulse generator to conduct an electroacupuncture stimulation experiment.

[0025] The electro-pulse generating device uses an electroacupuncture device, which is placed above the experimental animals. After the electrode clips of the electroacupuncture device are clamped onto the second conductive magnet, the connecting wires of the electrode clips extend upwards, with a redundant length reserved for the experimental animals' movements. When it is necessary to switch from a live stimulation experiment with applied electricity to a sham stimulation experiment without applied electricity, the electro-pulse generating device is turned off. When an individual experimental animal experiences an unexpected situation during stimulation due to electrical stimulation or social factors among the experimental animals, and the experiment needs to be temporarily interrupted, the experimental animal is grasped and the second conductive magnet is directly pulled off to disconnect the electrical stimulation. When conducting electroacupuncture stimulation experiments on multiple batches of experimental animals consecutively, while the previous batch of experimental animals is undergoing the electrical stimulation experiment, steps S1 to S3 are performed on the next batch of experimental animals. After the electroacupuncture stimulation of the previous batch of experimental animals is completed, the second conductive magnet is disconnected and connected to the first conductive magnet of the next batch of experimental animals.

[0026] In summary, the present invention has the following advantages:

[0027] (1) Reliable fixation: The tip of the needle body of the present invention has a backward tilt angle of 15° to 30°, which is adapted to the direction of the Du meridian, the Bladder meridian of Foot Taiyang, and the Huatuo Jiaji acupoints of the animal, thus solving the problem that the needle body is easy to fall over and fall off in small parts; the clamping arm and the needle body form a triangular stable support, ensuring that the device is not easy to fall off during the animal's activities.

[0028] (2) Prevent needle displacement or detachment: The magnetic attraction of the first and second conductive magnets can be easily set to be less than the external pulling force that would cause the needle to detach. In this way, even if the external conductive wire is accidentally pulled, the needle will not detach and will not cause additional damage to the experimental animal, making it convenient to continue the experiment.

[0029] (3) Freedom of movement and reduced hindrance: By using a top magnetic electrical connection and placing the external electroacupuncture device above the animal's activity plane, the external wire is kept upright and suspended, which greatly reduces the hindrance and drag of the cable on the animal's movement, allowing the animal to move freely during stimulation, and significantly improving the ecological validity and data quality of combined behavioral experiments.

[0030] (4) Precise and controllable, highly standardized: By adjusting the plate, hinge and needle body, the acupuncture angle and depth and acupoint position are standardized and controlled, greatly reducing the error of manual acupuncture and significantly reducing the operation error between different experimenters and different batches.

[0031] (5) Convenient comparison and blind method: The magnetic electric connection can realize the quick connection and disconnection with the electroacupuncture instrument, which is especially convenient for multiple batches of experimental operations, greatly improving experimental efficiency. Moreover, compared with the existing electroacupuncture instruments that use electrode clips to hold the electroacupuncture needles, the magnetic connection can directly connect to the universal electroacupuncture instrument without replacing the commonly used universal electroacupuncture instrument, making it more adaptable.

[0032] (6) High versatility and multiple uses: The invention can adjust the relative angle through the hinge, so that the device can be flexibly adapted to various acupoints (combinations) of experimental animals of various body sizes, such as mice, rats and rabbits, and realize multiple uses.

[0033] (7) Lightweight structure: Each component is made of lightweight materials, which reduces the burden and stress on animals and is suitable for experimental research with long-term and dynamic observation. Attached Figure Description

[0034] Figure 1 This is a front view schematic diagram of the electroacupuncture stimulation experimental device for laboratory animals according to the present invention.

[0035] Figure 2 This is a top view schematic diagram of the electroacupuncture stimulation experimental device for laboratory animals according to the present invention.

[0036] Figure 3 This is a bottom view of the electroacupuncture stimulation experimental device for laboratory animals according to the present invention.

[0037] Figure 4 This is a side view of the electroacupuncture stimulation experimental device for laboratory animals according to the present invention.

[0038] Among them, 1 is the base plate, 2 is the clamping arm, 3 is the adjustment plate, 4 is the needle body, 5 is the second conductive magnet, 6 is the clamping part, 7 is the airbag, 8 is the second conductive magnet, 9 is the arc-shaped concave surface, 10 is the pin hole, and 11 is the hinge. Detailed Implementation

[0039] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0040] Example 1

[0041] like Figures 1-4 As shown, an electroacupuncture stimulation experimental device for laboratory animals includes a base plate, a needle body, and an electrical connection assembly. Clamping arms are installed on the left and right sides of the base plate for clamping and fixing the laboratory animals. Multiple adjustment plates are connected to the bottom of the base plate. The lower surface of the adjustment plate is a concave surface for conforming to the laboratory animals. The needle body is installed in the adjustment plate, and the needle tip of the needle body protrudes from the lower surface of the adjustment plate. The electrical connection assembly includes a first conductive magnet and a second conductive magnet that can magnetically attract each other. The first conductive magnet is fixed to the base plate and electrically connected to the needle body. The second conductive magnet is used to connect to an external electrical pulse generator.

[0042] The needle body is an electrocautery needle for electrical stimulation. Multiple adjustment plates are arranged sequentially along the left-right direction, forming a semi-enclosed structure adapted to the animal's body surface. The central adjustment plate is fixedly connected to the base plate. During the electrical stimulation experiment, because the adjustment plates are in contact with the experimental animal's body surface, the insertion depth and angle are limited by the protruding length and angle of the needle tip, thus achieving controllable insertion depth and angle.

[0043] Specifically, the lower surface of the adjusting plate is an arc-shaped concave surface that curves in the left-right direction and extends linearly in the front-back direction. The needle body is fixedly connected to the adjusting plate, and the needle tip is tilted backward at 15°~30°. The adjusting plate has a needle hole for inserting the needle body, such as... Figure 4 As shown in the figure, angle a ranges from 15° to 30°. The specific angle and protrusion length of the needle body are designed according to requirements. This angle is adapted to the direction of the Du meridian in animals to ensure that the needle body is naturally stable after insertion.

[0044] Specifically, multiple adjustment plates are arranged sequentially in a left-right direction. The number and size of the adjustment plates are determined according to the body size of the experimental animal. Adjacent adjustment plates are hinged to adjust the angle between them. An angle locking mechanism is provided between adjacent adjustment plates to lock them in place after the angle is adjusted. Adjacent adjustment plates are connected by hinges, and the angle locking mechanism can be an existing one. Alternatively, the angle locking mechanism includes elastic claws and multiple positioning slots. The elastic claws are located on the hinge's pivot and rotate with the pivot. The multiple positioning slots are arranged sequentially on the hinge's fixed component along the rotation trajectory of the elastic claws, according to the opening and closing angle adapted to the experimental animal's body size. When the hinge rotates to an angle adapted to the target animal's body size, the elastic claws, under their own elastic force, engage and lock into the corresponding positioning slots, thus completing the rapid positioning and fixing of the opening and closing angle between two adjacent adjustment plates.

[0045] The locking between the elastic pawl and the positioning slot can be bidirectional locking or unidirectional locking in the form of a pawl and ratchet. In the case of unidirectional locking, a torsion spring is also provided between two adjacent adjusting plates. The elastic torque of the torsion spring on the rotating shaft component is opposite to the direction of unidirectional locking.

[0046] In another embodiment, adjacent adjustment plates are hinged together by damped hinges with rotational damping, requiring a certain torque to overcome the damping rotation, without the need for an additional angle locking mechanism.

[0047] Specifically, both the adjustment plate and the base plate are made of rigid plastic. The first conductive magnet is embedded in the base plate. The lower surface of the first conductive magnet is connected to the needle body via a conductive wire, while its upper surface is exposed to attract the second conductive magnet. The second conductive magnet has a clamping part for holding the electrode clip of the power pulse generator. Both the first and second conductive magnets are made of nickel-neodymium iron-boron magnets, are cylindrical, and have an insulating coating on their outer circumference. The first conductive magnet is embedded in a cutout in the base plate, protruding from the upper and lower surfaces of the base plate. The conductive wire connects the first conductive magnet to the needle body. The second conductive magnet is an independent component, with a clamping part for connecting the electrode clip or wire end of an external electroacupuncture device. When the second conductive magnet and the first conductive magnet are magnetically attracted, the circuit is connected; separating the second conductive magnet from the first conductive magnet instantly cuts off the power.

[0048] Specifically, the magnetic attraction between the first and second conductive magnets is configured such that when the second conductive magnet is pulled, it separates from the first conductive magnet before the needle begins to detach or shift from the surface of the experimental animal, thus forming a protection against needle detachment. Since a connecting wire is needed to the electrical pulse generator in the electroacupuncture stimulation experiment, the movement of the experimental animal can cause the connecting wire to be pulled. Therefore, in addition to the robustness of the device, a further anti-detachment design is implemented so that even a large accidental pull will not cause the needle to detach. The minimum pulling force on the second conductive magnet that would cause detachment or shift from the surface of the experimental animal can be obtained through experience or theory, and the magnetic attraction force is made to be less than this minimum pulling force.

[0049] Specifically, the end of the gripping arm is equipped with a pre-inflated airbag or an instant-inflated airbag for contacting the experimental animal; the pre-inflated airbag is a fully sealed airbag filled with air; the instant-inflated airbag is equipped with an inflation port for inflating the airbag after the gripping arm has initially gripped the experimental animal.

[0050] When using pre-inflated airbags, the pre-inflated airbags adapt to the surface of the experimental animal through their own deformation and elasticity during clamping, providing cushioning and increasing the contact area. When using instant-inflated airbags, the airbags are not inflated before clamping. After the clamping arms initially clamp the experimental animal, the airbags are inflated to provide cushioning, and the clamping pressure is controlled by adjusting the inflation volume. The use of airbags not only increases the stability of the clamping but also improves the comfort of the experimental animal.

[0051] Specifically, the clamping arm is a retractable, arc-shaped, elastic arm. During the experiment, the clamping arm is first retracted. After the needle pierces the surface of the experimental animal, the clamping arm is then pulled open to clamp the animal on both sides, using a pre-inflated airbag for cushioning and clamping; alternatively, the airbag is inflated, positioned between the end of the clamping arm and the experimental animal, until the required clamping pressure is achieved. The arc-shaped elastic arm has a two-section telescopic structure, including an arc-shaped fixed arm and a movable arm. One end of the fixed arm is connected to the base plate, and the other end is telescopically connected to the movable arm, allowing for retraction and storage through the extension and retraction of the movable arm.

[0052] In another embodiment, the clamping arm is a non-retractable elastic arm that is arc-shaped, made of metal, with its head fixed to the base plate and its end connected to an air bladder, clamping the experimental animal while the needle pierces its body surface.

[0053] Specifically, the number of needles is 1 to 3, used for single-acupoint, double-acupoint, or multi-acupoint stimulation experiments. When the number of needles is 2 or more, the number of electrical connection components is 2, with two second conductive magnets used to connect to the positive and negative electrode clips of the external electrical pulse generator, respectively. For example... Figure 1 and 3As shown, there are 3 needles arranged from left to right. The first conductive magnets of the two electrical connection components are electrically connected to the needles on the left and right sides, respectively. When the electrical pulse generator is turned on, the current flows from the left needle, through the experimental animal body and the middle needle, to the left needle to form an electrical stimulation circuit.

[0054] Example 2

[0055] An electroacupuncture stimulation experiment method for laboratory animals, using the electroacupuncture stimulation experiment device for laboratory animals according to Example 1, includes the following steps:

[0056] S1. Adjust the angle between adjacent adjustment plates according to the size of the experimental animal so that the enclosed shape of multiple adjustment plates matches the surface of the experimental animal.

[0057] S2, bring the adjustment plate close to the surface of the experimental animal, align the needle with the corresponding acupoint, and insert the needle into the acupoint at the angle of the needle tip;

[0058] S3, the experimental animal is held and fixed by the clamping arm; at this time, the clamping arm and the needle after insertion together form a triangular stable support structure, so that the device is firmly fixed to the animal's body surface.

[0059] S4, the second conductive magnet is attracted to the first conductive magnet, and the electric pulse generator is connected to the second conductive magnet, so that the electric pulse generator is electrically connected to the needle body;

[0060] S6, turn on the electrical pulse generator to conduct an electroacupuncture stimulation experiment.

[0061] In step S2, the clamping arm is retracted. In step S3, when an instant-inflation airbag is used, the airbag is first deflated, and the experimental animal is initially clamped by the clamping arm. Then, the instant-inflation airbag is inflated to form a buffer between the clamping arm and the experimental animal, while increasing the contact area. The clamping pressure is adjusted by the amount of inflation.

[0062] The electrical pulse generator uses an electroacupuncture device, which is positioned above the experimental animal. The electrode clips of the electroacupuncture device are attached to the second conductive magnet, with the connecting wires of the electrode clips extending upwards, allowing for redundant length for the experimental animal's movement. When switching from a live stimulation experiment to a sham stimulation experiment, the electrical pulse generator is turned off. If an individual experimental animal (mice) experiences an unexpected situation during stimulation due to electrical stimulation or social factors among the animals, requiring temporary interruption of the experiment, the animal is grasped and the second conductive magnet is directly pulled off to disconnect the electrical stimulation. This operation is convenient and does not affect other mice.

[0063] When conducting electroacupuncture stimulation experiments on multiple batches of experimental animals, steps S1 to S3 are performed simultaneously with the current batch of animals undergoing electroacupuncture stimulation. After the electroacupuncture stimulation of the previous batch of animals is completed, the second conductive magnet is disconnected and connected to the first conductive magnet of the next batch of animals. In this way, only two batches of the above-mentioned electroacupuncture stimulation experimental device need to be prepared to conduct continuous electroacupuncture stimulation experiments, eliminating the time spent on intermediate puncture fixation and connecting wires, making the operation convenient and improving experimental efficiency.

[0064] This invention is rationally designed, simple in structure, and easy to operate. It can be widely used in animal experiments in brain science and acupuncture mechanism research, and is especially suitable for precise electroacupuncture intervention in research paradigms such as behavioral science and electrophysiology. It has good industrial practicality and promotional application value.

[0065] The above embodiments are preferred embodiments of the invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. An electroacupuncture stimulation experimental device for laboratory animals, characterized in that: Includes base plate, needle body and electrical connection assembly; Clamping arms are installed on the left and right sides of the base plate for clamping and fixing the experimental animals. Multiple adjustment plates are connected to the bottom of the base plate. The lower surface of the adjustment plate is a concave surface for fitting the experimental animals. The needle body is installed in the adjustment plate, and the needle tip protrudes from the lower surface of the adjustment plate. The electrical connection assembly includes a first conductive magnet and a second conductive magnet that can magnetically attract each other. The first conductive magnet is fixed to the base plate and electrically connected to the needle body, and the second conductive magnet is used to connect to an external electrical pulse generator.

2. The electroacupuncture stimulation experimental device for laboratory animals according to claim 1, characterized in that: The lower surface of the adjusting plate is an arc-shaped concave surface that curves in the left-right direction and extends in a straight line in the front-back direction. The needle body is fixedly connected to the adjusting plate, and the needle tip is tilted backward at 15°~30°.

3. The electroacupuncture stimulation experimental device for laboratory animals according to claim 1, characterized in that: Multiple adjustment plates are arranged sequentially in the left-right direction. The number and size of the adjustment plates are determined according to the body size of the experimental animal. Adjacent adjustment plates are hinged to adjust the angle between them. An angle locking mechanism is provided between adjacent adjustment plates to lock them after the angle is adjusted.

4. The electroacupuncture stimulation experimental device for laboratory animals according to claim 1, characterized in that: Both the adjustment plate and the base plate are made of rigid plastic. The first conductive magnet is embedded in the base plate. The lower surface of the first conductive magnet is connected to the needle body through a conductive wire, and the upper surface is exposed to attract the second conductive magnet. The second conductive magnet is provided with a clamping part to be clamped by the electrode clamp of the power pulse generator.

5. The electroacupuncture stimulation experimental device for laboratory animals according to claim 1, characterized in that: The magnetic attraction between the first and second conductive magnets is configured such that when the second conductive magnet is pulled, it separates from the first conductive magnet before the needle begins to detach or shift from the surface of the experimental animal, thus forming a protection against needle detachment.

6. The electroacupuncture stimulation experimental device for laboratory animals according to claim 1, characterized in that: The end of the gripping arm is equipped with a pre-inflated or instant-inflated airbag for contacting the experimental animal. A pre-inflated airbag is a fully inflated, sealed airbag. The instant-inflatable airbag has an inflation port for inflating the airbag after the gripping arm has initially gripped the experimental animal.

7. The electroacupuncture stimulation experimental device for laboratory animals according to claim 1, characterized in that: The clamping arm is a retractable, curved, flexible arm.

8. The electroacupuncture stimulation experimental device for laboratory animals according to claim 1, characterized in that: The number of needles is 1 to 3, and they are used for single, double or multiple acupoint stimulation experiments.

9. A method for electroacupuncture stimulation experiments on laboratory animals, using the electroacupuncture stimulation experiment apparatus for laboratory animals as described in any one of claims 1 to 8, characterized in that: Includes the following steps, S1. Adjust the angle between adjacent adjustment plates according to the size of the experimental animal so that the enclosed shape of multiple adjustment plates matches the surface of the experimental animal. S2, bring the adjustment plate close to the surface of the experimental animal, align the needle with the corresponding acupoint, and insert the needle into the acupoint at the angle of the needle tip; S3, the experimental animal is held and fixed by the clamping arm; S4, the second conductive magnet is attracted to the first conductive magnet, and the electric pulse generator is connected to the second conductive magnet, so that the electric pulse generator is electrically connected to the needle body; S6, turn on the electrical pulse generator to conduct an electroacupuncture stimulation experiment.

10. The method for electroacupuncture stimulation experiments on laboratory animals according to claim 9, characterized in that: The electric pulse generator uses an electroacupuncture device. The electroacupuncture device is placed above the experimental animal. After the electrode clip of the electroacupuncture device is clipped onto the second conductive magnet, the connecting wire of the electrode clip is extended upwards, with a redundant length reserved for the experimental animal's movement. When it is necessary to switch from a live stimulus experiment with electricity to a sham stimulus experiment without electricity, turn off the electrical pulse generator; When an individual experimental animal needs to be temporarily interrupted during stimulation due to an unexpected situation during stimulation caused by electrical stimulation or social factors among experimental animals, the second conductive magnet is pulled off directly after the experimental animal is grasped to disconnect the electrical stimulation. When conducting electroacupuncture stimulation experiments on multiple batches of experimental animals, while the previous batch of experimental animals is being electrostimulated, steps S1 to S3 are performed on the next batch of experimental animals. After the electroacupuncture stimulation of the previous batch of experimental animals is completed, the second conductive magnet is disconnected and connected to the first conductive magnet of the next batch of experimental animals.

Images