A nanoscale enzyme biosensor and a preparation method and application thereof

Abstract

The application belongs to the technical fields of nanobiological sensing, food microorganism detection and analytical chemistry, and relates to a nano-enzyme biosensor and a preparation method and application thereof. The nano-enzyme biosensor comprises a bacteria recognition unit, an entropy-driven signal amplification unit and a dual-mode signal detection unit, wherein the entropy-driven signal amplification unit contains a ternary nano-enzyme, the ternary nano-enzyme takes ZIF-67 as a substrate, internally loads CaO2 and surface loads Ag NPs, and the structure of the ternary nano-enzyme is a tubular structure. The ternary nano-enzyme is constructed by combining ZIF-67, silver nanoparticles and CaO2, a dual-mode biosensor is constructed by coupling an entropy-driven chain displacement reaction, high-sensitivity and high-specificity detection of Staphylococcus aureus is realized, and the biosensor simultaneously has the functions of long-acting luminescence, cross verification and bacteria inactivation, so that the shortcomings of existing detection technologies can be made up.

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