Glucosyl soil detoxification amendment and its soil decontamination applications

Abstract

The application discloses a soil purification application of a glucose-based soil detoxification and improvement agent, and realizes efficient purification by applying the improvement agent with a specific formula to contaminated soil.The improvement agent comprises alkyl polyglycoside, humic acid and mineral trace elements.The scheme utilizes alkyl polyglycoside (C8-C14) to solubilize and disperse organic phosphorus or triazine pesticides, improves the biological availability of pollutants; meanwhile, the scheme utilizes humic acid and alkyl polyglycoside to cooperatively provide a composite carbon source, stimulates in-situ proliferation of beneficial microorganisms such as Pseudomonas and / or Bacillus, and activates microbial enzyme activity through mineral trace elements.The application realizes the synergy of soil detoxification and improvement of soil fertility, and has the advantages of simple process, low cost, environmental friendliness and stable repair effect.

Description

Technical Field

[0001] This invention relates to the field of soil remediation and improvement technology, specifically to a glucose-based soil detoxification and improvement agent and its soil purification application. Background Technology

[0002] With the rapid development of modern agriculture, the widespread use of pesticides, while ensuring grain yields, has also brought about serious soil pollution problems. Among them, organophosphates and triazine pesticides, due to their stable chemical properties and long half-lives, easily accumulate in the soil, not only disrupting the ecological balance of the soil but also potentially posing a threat to the ecological environment and human health through bioaccumulation in the food chain.

[0003] Currently, remediation technologies for pesticide residue-laden soils mainly include physical remediation (such as topsoil replacement and thermal desorption), chemical remediation (such as redox and photocatalytic degradation), and bioremediation (such as microbial degradation and phytoremediation). While physical and chemical remediation methods are fast-acting, they are often costly and can easily cause secondary damage to soil structure and existing microbial communities. Bioremediation technology has attracted much attention due to its environmental friendliness and lower cost, but its remediation cycle is long and greatly affected by environmental factors (such as temperature, pH, and nutrients), resulting in unstable effectiveness.

[0004] In the prior art, CN201710559813.8 discloses a soil conditioner containing chelated calcium and its preparation method. This technology involves activating and culturing Bacillus subtilis, and then fermenting and drying it with calcium carbonate and other components. Although this technology can improve soil to some extent using microorganisms, it has the following significant drawbacks: 1. This technology involves complex processes such as multi-step cultivation (solid culture, seed liquid culture, fermentation culture), high-temperature sterilization, and fluidized bed drying. It is not only energy-intensive, but also has stringent requirements for production equipment, making it difficult to achieve large-scale low-cost production.

[0005] 2. This technology relies solely on a single species, Bacillus subtilis, and lacks consideration of the synergistic effects of multiple beneficial bacterial groups (such as Pseudomonas). Furthermore, exogenous bacterial agents are easily antagonized by native microorganisms when introduced into complex and variable soil environments, leading to difficulties in colonization, low survival rates, and unstable remediation effects.

[0006] 3. This technology focuses on the preparation of microbial agents and does not involve methods for solubilizing, dispersing, or activating pesticide residues in the soil. For organophosphates or triazine pesticides that are firmly adsorbed by the soil, relying solely on natural contact degradation by microorganisms is extremely inefficient.

[0007] 4. This technology is mainly aimed at soil structure improvement (calcium supplementation) and the prevention and control of specific diseases. It does not provide a specific solution for pesticide pollution with high residues and difficulty in degradation, and its ability to directly remove pesticide residues is limited.

[0008] Therefore, developing a soil purification technology that can efficiently degrade pesticide residues, simultaneously improve soil, promote the synergistic proliferation of various beneficial microbial communities, and is simple to prepare and inexpensive is a technical challenge that urgently needs to be solved in the field of soil remediation. Summary of the Invention

[0009] The purpose of this invention is to overcome the shortcomings of the prior art and provide a glucose-based soil detoxification amendment and its soil purification application. Through the precise combination of alkyl glycosides with specific carbon chain lengths, humic acid, and trace element chelates in a specific ratio, the invention solves the problems of difficult soil pesticide desorption and low carbon source matching degree of beneficial microorganisms in the prior art.

[0010] To solve the above-mentioned technical problems, the technical solution provided by the present invention is: a soil purification application of a glucose-based soil detoxification amendment, comprising the following method, the specific steps of which are as follows: Apply glucose-based soil detoxification amendments to soils containing organophosphate or triazine pesticide residues; The glucose-based soil detoxification amendment contains alkyl glycosides, humic acid, and trace mineral elements; Wherein, the alkyl glycoside has an alkyl carbon chain length of C8-C14; The method achieves a synergistic promotion of pesticide residue degradation and the proliferation of beneficial microbial communities of Pseudomonas and / or Bacillus through the solubilizing and dispersing effect of the alkyl glycoside on pesticide molecules, and the carbon source and growth stimulation environment provided by the humic acid and alkyl glycoside.

[0011] Furthermore, in the glucose-based soil detoxification amendment, the weight ratio of the alkyl glycoside to humic acid is 1:4 to 4:1. More preferably, the alkyl carbon chain length of the alkyl glycoside is C8-C10, preferably octyl glucoside, decyl glucoside, or a mixture of the two.

[0012] Furthermore, the mineral trace elements include a chelate of zinc, molybdenum, and iron, and the weight ratio of zinc, molybdenum, and iron is (1-3):(0.1-0.5):(1-2).

[0013] In one specific embodiment, the glucose-based soil detoxification amendment comprises, by weight: 20-50 parts alkyl glycoside, 30-60 parts humic acid, and 5-15 parts trace mineral elements. The application rate is 5-20 kg per acre; preferably, the amendment is mixed evenly with the top 0-20 cm of soil during application; preferably, the composition is mixed evenly with the top 0-20 cm of soil during application.

[0014] In addition, the present invention provides a glucose-based soil detoxification amendment for use in the above method, comprising the following components in parts by weight: Alkyl glycosides 20-50 parts, humic acid 30-60 parts, mineral trace elements 5-15 parts; The alkyl glycoside has an alkyl carbon chain length of C8-C14, and the mineral trace elements include chelates of zinc, molybdenum, and iron.

[0015] Preferably, the alkyl glycoside is octyl glucoside or decyl glucoside. The humic acid is selected from one or more of mineral-derived humic acid and biochemical humic acid; preferably, it is mineral-derived potassium fulvate with a humic acid content ≥50%. The dosage form of the composition is a soluble powder or granules; preferably, the particle size of the granules is 1-4 mm.

[0016] The advantages of this invention compared to the prior art are: 1. The alkyl glycosides, humic acid and trace mineral elements used in this invention are all commercially available industrial raw materials. They do not require complex microbial fermentation, high-temperature sterilization and drying granulation, which are energy-intensive steps. The preparation process is simple, easy to produce on a large scale and promote application, and significantly reduces production costs.

[0017] 2. This invention does not rely on the addition of a single exogenous microbial strain. Instead, it activates and enriches various beneficial microorganisms, such as Pseudomonas and Bacillus, that are already present in the soil by providing a complex carbon source and trace elements. This "microbial-based microbial cultivation" strategy avoids the problem of difficult colonization of exogenous microbial strains. By utilizing the soil's own ecological balance, it constructs a more stable and efficient degradation microbial community, resulting in a more lasting remediation effect.

[0018] 3. This invention innovatively introduces alkyl glycosides as a solubilizer. It can effectively break the adsorption bonds between pesticide molecules and soil particles, "eluting" them out, greatly improving the bioavailability of pesticides, and thus significantly accelerating the degradation process.

[0019] 4. While effectively removing pesticide residues, humic acid significantly improves the physical structure and chemical fertility of the soil, and trace elements promote crop root growth, enabling the remediated soil to immediately restore high yield capacity, overcoming the limitations of the comparative documents that only focus on single improvement or prevention.

[0020] 5. All components used (alkyl glycosides, humic acid, and minerals) are environmentally friendly substances with no risk of secondary pollution, which is in line with the development concept of green agriculture. Detailed Implementation

[0021] To make the technical problems, solutions, and beneficial effects of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0022] Examples 1-3 and Comparative Examples 1-4

[0023] Test soil: Topsoil (0-20cm) from a farmland that has long been planted with corn. This soil was contaminated with a combination of chlorpyrifos (organophosphates) and atrazine (triazines). The basic soil properties were: pH 6.8, organic matter content 2.1%, sand:silt:clay = 40:35:25.

[0024] Soil conditioner preparation: Prepare the soil conditioners for Examples 1-3 and Comparative Examples 1-4 according to the components and proportions shown in the table below. All components are commercially available products.

[0025] Alkyl glycosides (APG): C8-10 APG (octyl / decyl glucoside), C12-14 APG (lauryl / myristyl glucoside).

[0026] Humic acid: mineral-derived potassium fulvate (humic acid content ≥50%).

[0027] Mineral trace elements: Zinc, molybdenum, and iron in EDTA chelated form.

[0028] Experimental Design: The test soil was air-dried, ground, and sieved, then evenly distributed into multiple culture pots. The soil amendments from Examples 1-3 and Comparative Examples 1-4 were applied at a rate of 10 kg per acre, and thoroughly mixed with the soil layer from 0 to 20 cm. A blank control group (CK) without any amendment was also included. Each treatment was replicated in triplicate. The soils were incubated at 25°C for 60 days, during which time the soil moisture content was maintained at 60% of field capacity.

[0029] Measurement indicators: Pesticide degradation rate: On day 60 of cultivation, the content of residual chlorpyrifos and atrazine in the soil was determined by gas chromatography, and the degradation rate was calculated.

[0030] Beneficial microorganism count: The number of colony-forming units (CFU) of Pseudomonas and Bacillus in the soil was determined by the dilution plating method.

[0031] Table 1: Component formulations (parts by weight) for each embodiment and comparative example

[0032] Table 2: Comparison of repair effects of each treatment group after 60 days

[0033] Results analysis: Compared with the blank control (CK), all treatment groups treated with the amendment showed some pesticide degradation and microbial promotion, demonstrating the effectiveness of the amendment.

[0034] Example 1 (C8-C10 APG, specific ratio, containing trace elements) exhibited the best synergistic effect, with significantly higher pesticide degradation rate and beneficial bacteria count than other groups. This indicates that C8-C10 alkyl glycosides are superior to C12-C14 (Comparative Example 3) in solubilization, and the synergistic effect of the three components is crucial.

[0035] The results of Comparative Examples 1 and 2 show that the effect of using humic acid or alkyl glycoside alone is much lower than that of using them together (Example 1), demonstrating that alkyl glycosides and humic acid have a significant synergistic effect in providing carbon source and growth stimulation.

[0036] The results of Comparative Example 3 showed that the lack of mineral trace elements significantly reduced the repair effect, indicating that trace elements play an irreplaceable role in activating microbial enzyme activity and accelerating the degradation process.

[0037] Comparative Example 4, which replaced alkyl glycosides with ordinary glucose, showed poor results. This demonstrates that alkyl glycosides not only serve as a carbon source, but their unique surface activity (solubilizing and dispersing effect) is also key to achieving efficient degradation.

[0038] The present invention and its embodiments have been described above. This description is not restrictive. In short, if a person skilled in the art is inspired by this description and designs a similar structure and embodiment without departing from the spirit of the present invention, such design should fall within the protection scope of the present invention.

Claims

1. A soil purification application of a glucose-based soil detoxification amendment, characterized in that, The methods include the following steps: Apply glucose-based soil detoxification amendments to soils containing organophosphate or triazine pesticide residues; The glucose-based soil detoxification amendment contains alkyl glycosides, humic acid, and trace mineral elements; Wherein, the alkyl glycoside has an alkyl carbon chain length of C8-C14; The method achieves a synergistic promotion of pesticide residue degradation and the proliferation of beneficial microbial communities of Pseudomonas and / or Bacillus through the solubilizing and dispersing effect of the alkyl glycoside on pesticide molecules, and the carbon source and growth stimulation environment provided by the humic acid and alkyl glycoside.

2. The soil purification application according to claim 1, characterized in that, In the glucose-based soil detoxification amendment, the weight ratio of the alkyl glycoside to humic acid is 1:4 to 4:

1.

3. The soil purification application according to claim 1 or 2, characterized in that, The alkyl glycoside has an alkyl carbon chain length of C8-C10, preferably octyl glucoside, decyl glucoside, or a mixture of the two.

4. The soil purification application according to claim 1, characterized in that, The mineral trace elements include a chelate of zinc, molybdenum and iron, and the weight ratio of zinc, molybdenum and iron is (1-3):(0.1-0.5):(1-2).

5. The soil purification application according to claim 1, characterized in that, The glucose-based soil detoxification amendment comprises, by weight: Alkyl glycosides 20-50 parts, 30-60 parts humic acid 5-15 parts of minerals and trace elements.

6. The soil purification application according to claim 1, characterized in that, The application rate of the glucose-based soil detoxification amendment is 5-20 kg per acre; preferably, the composition is mixed evenly with the top 0-20 cm of soil during application.

7. A glucose-based soil detoxification amendment for use in the method of any one of claims 1 to 6, characterized in that, It consists of the following components in parts by weight: Alkyl glycosides 20-50 parts, 30-60 parts humic acid 5-15 parts of mineral trace elements; The alkyl glycoside has an alkyl carbon chain length of C8-C14, and the mineral trace elements include chelates of zinc, molybdenum, and iron.

8. The glucose-based soil detoxification amendment according to claim 7, characterized in that, The alkyl glycoside is octyl glucoside or decyl glucoside.

9. The glucose-based soil detoxification amendment according to claim 7, characterized in that, The humic acid is selected from one or more of mineral-derived humic acid and biochemical humic acid; preferably, it is mineral-derived potassium fulvate, with a humic acid content ≥50%.

10. The glucose-based soil detoxification amendment according to claim 7, characterized in that, The dosage form of the composition is a soluble powder or granules; preferably, the particle size of the granules is 1-4 mm.

Images