Temperature control device for cell culture medium preservation

Abstract

The application discloses a kind of cell culture medium storage temperature control device, it is applied to cell culture medium storage temperature control device technical field, including incubator, temperature control backplate, storage mechanism and temperature regulating mechanism, the temperature control backplate, storage mechanism, temperature regulating mechanism are all set in incubator, the temperature control backplate is fixedly connected with incubator, the storage mechanism includes several partition components, several drive assemblies and several storage components, the temperature regulating mechanism includes several flow guide components, several shunt components and several temperature sensors, the temperature sensor is fixed on the inner wall of storage grid;The partition component includes layer plate and partition plate, the storage component includes storage grid and storage plate, the flow guide component includes flow guide plate, drive part, push plate, sliding block one, spring two, top plate and spring three, the present application can realize high-precision, high uniformity and fast response temperature control, ensure that the temperature of cell culture medium keeps stable.

Description

Technical Field

[0001] This invention relates to the technical field of temperature control devices for preserving cell culture media, specifically a temperature control device for preserving cell culture media. Background Technology

[0002] Cell culture media are indispensable core reagents in biopharmaceutical, tissue engineering, and basic medical research, and their quality and stability directly determine the success or failure of cell culture experiments. These media are rich in heat-sensitive components such as amino acids, vitamins, growth factors, and buffer salts, making them extremely sensitive to storage temperature. They typically need to be strictly maintained in a specific low-temperature environment of 2°C to 8°C or below -20°C.

[0003] However, existing commercially available medical refrigerators and freezers are limited in practical use by traditional single-point temperature measurement and simple on / off control logic. This results in poor temperature uniformity within the unit, with localized overcooling near the evaporator and higher temperatures at the door and edges, with temperature differences exceeding 3°C. Furthermore, frequent door opening and closing, compressor start-up and shutdown, and fluctuations in the external environment often cause drastic temperature fluctuations, with instantaneous fluctuations exceeding ±2°C. These temperature instabilities accelerate L-glutamine degradation, cause pH shifts and precipitation in culture media, and can even lead to complete media inactivation.

[0004] Therefore, it is necessary to provide a temperature control device for preserving cell culture medium to solve the above problems. Summary of the Invention

[0005] The purpose of this invention is to provide a temperature control device for preserving cell culture medium, which can achieve high precision, high uniformity and rapid response temperature control, and ensure that the temperature of the cell culture medium is kept stable, so as to solve the problems mentioned in the background art.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a temperature control device for preserving cell culture medium, comprising an insulated box, a temperature control backplate, a storage mechanism, and a temperature adjustment mechanism. The temperature control backplate, the storage mechanism, and the temperature adjustment mechanism are all disposed inside the insulated box. The temperature control backplate is fixedly connected to the insulated box. The storage mechanism is disposed on one side of the temperature control backplate. The storage mechanism includes a plurality of partition components, a plurality of drive components, and a plurality of storage components. The temperature adjustment mechanism includes a plurality of flow guiding components, a plurality of flow splitting components, and a plurality of temperature sensors. The number of partition components, drive components, storage components, flow guiding components, flow splitting components, and temperature sensors are the same and their positions correspond. The temperature sensors are fixed on the inner wall of the storage compartment. The separating component includes a shelf and a partition plate; the storage component includes a storage compartment and a storage plate; the flow guiding component includes a flow guiding plate, a driving component, a push plate, a sliding block one, a spring two, a top plate, and a spring three. The flow guiding plate is located at the top inside the storage compartment. The shelf has a flow outlet in the vertical direction. The shelf has a sliding groove in the horizontal direction at one end near the temperature control back panel. The flow outlet passes through the sliding groove. The push plate is fixedly connected to the bottom of the sliding block one. The top plate is in contact with the sliding block one. The spring two and spring three are located at the two ends of the sliding groove, respectively.

[0007] According to the above technical solution, the partition plate is fixed on the top of the shelf, and the driving component and the storage component are disposed on the top of the shelf and respectively disposed on both sides of the partition plate.

[0008] According to the above technical solution, the driving component includes a cylinder, an adsorption plate, and several suction cups. The cylinder is disposed on one side of the partition plate and fixed to the top of the shelf. The adsorption plate is fixed to the output end of the cylinder. Several suction cups are fixed on the side of the adsorption plate away from the cylinder. Several suction cups are connected to an air extraction device via pipes.

[0009] According to the above technical solution, the storage plate is disposed at the top inside the storage compartment, and the storage compartment is disposed on the side of the partition plate away from the cylinder; The storage compartment is provided with a reflux port on its side, and the reflux port pipe is connected to an air extraction device. The bottom of the storage compartment near the temperature control back panel is provided with a guide port. The storage compartment near the temperature control back panel is provided with several air inlets at equal intervals on its side, and several air inlets are provided at equal intervals along the circumferential direction of the air inlets. A connecting plate is fixedly connected to the side of the storage compartment near the cylinder. The connecting plate is located near the door of the box and its position corresponds to that of the adsorption plate.

[0010] According to the above technical solution, the temperature control back panel is provided with several sets of air outlets, the number of sets of air outlets corresponds to the number of storage compartments, and the number and position of each set of air outlets corresponds to the number and position of air inlets. The inner diameter of the air outlet matches the outer diameter of the air inlet. A top cover is slidably connected to the air outlet. Several air outlet holes are equidistantly arranged on the top cover along the circumference of the top cover. A plug is fixedly connected to the side of the top cover facing the air outlet. The outer diameter of the plug matches the inner diameter of the air outlet. A spring is fitted onto the air outlet, and the two ends of the spring are fixedly connected to the inner walls of the top cover and the temperature control back plate, respectively.

[0011] According to the above technical solution, the guide plate is rotatably connected to the storage compartment, the side wall of the storage compartment is fixedly connected to a support, the driving component is fixed on the support, and the output end of the driving component passes through the support and is connected to the guide plate for transmission.

[0012] According to the above technical solution, the sliding block one, spring two, top plate and spring three are all arranged in the sliding groove. The sliding block one is U-shaped and the top plate is L-shaped. The two ends of the spring two are fixedly connected to the sliding block one and the shelf respectively. The two ends of the spring three are fixedly connected to the temperature control back plate and the top plate respectively. The storage compartment has a push-press groove on the side near the temperature control back panel, and the size and position of the push-press groove are matched with the push plate.

[0013] According to the above technical solution, the sliding block is provided with a through-hole 1, and the top plate is provided with a through-hole 2. The through-hole 1, through-hole 2, the drain port and the guide port 1 are the same size.

[0014] According to the above technical solution, the diversion component includes two sets of telescopic components, a second sliding block, several columns and several fan wheels. The two sets of telescopic components are located on both sides of the second sliding block. The two ends of the telescopic components are fixedly connected to the second sliding block and the storage compartment, respectively. The second sliding block is slidably connected to the storage compartment. The number of air inlets on the columns, fan wheels and storage compartment is the same.

[0015] According to the above technical solution, the column is fixed on the top of the sliding block 2, the spacing between the columns is the same as the spacing between the air inlets on the storage compartment, the fan wheel is set on the top side wall of the column, the fan wheel is rotatably connected to the column, and the sliding block 2 is provided with a plurality of guide ports 2, the guide ports 2 being located directly below the fan wheel.

[0016] Compared with the prior art, the beneficial effects achieved by the present invention are as follows: The present invention, by setting the air outlet, top cover, plug, spring 1 on the temperature control back plate, and the air inlet and air inlet hole on the storage compartment, can realize automatic connection of air supply when the storage compartment is pushed in and automatic sealing of the air outlet when pulled out, so as to prevent cold air from leaking out, reduce energy consumption, and at the same time ensure the heat preservation effect in the non-working state. By incorporating a flow-diverting component, it is possible to achieve uniform airflow dispersion or targeted cooling, and further promote temperature uniformity by dynamically adjusting the position of the fan wheel.

[0017] By incorporating flow guiding components and flow inlets and sliding grooves on the separating components, along with the push grooves and flow guiding ports on the storage compartments, independent temperature adjustment of a single compartment or temperature equalization between layers can be achieved to meet different temperature control requirements. This results in high-precision, high-uniformity, and rapid-response temperature control, ensuring that the cell culture medium remains at a stable temperature during storage and meeting the stringent temperature requirements of cell culture. Attached Figure Description

[0018] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings: Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the internal structure of the present invention; Figure 3 This is a side view of the internal structure of the present invention; Figure 4 This is a partial structural schematic diagram of the present invention; Figure 5 This is the invention Figure 4 Front sectional view; Figure 6 This is the invention Figure 4 Enlarged structural diagram of region A in the middle; Figure 7 This is the invention Figure 7 Enlarged structural diagram of region B in the middle; Figure 8 This is the invention Figure 7 Enlarged structural diagram of region C in the middle; Figure 9 This is an exploded view of part of the temperature control mechanism of the present invention; Figure 10 This is an exploded view of part of the structure of the present invention; In the diagram: 1. Insulated box; 11. Box door; 2. Temperature control back panel; 21. Air vent; 22. Top cover; 23. Air outlet; 24. Plug; 25. Spring 1; 3. Separator assembly; 31. Shelf; 32. Separator plate; 33. Drainage port; 34. Sliding groove; 4. Drive assembly; 41. Cylinder; 42. Adsorption plate; 43. Suction cup; 5. Storage assembly; 51. Storage compartment; 52. Storage plate; 53. Return port; 54. Drainage port one; 55. Air inlet; 56. Air inlet hole; 57. Connecting plate; 58. Push groove; 6. Flow guiding assembly; 61. Flow guiding plate; 62. Support; 63. Drive component; 64. Push plate; 65. Sliding block one; 651. Through port one; 66. Spring two; 67. Top plate; 671. Through port two; 68. Spring three; 7. Diverting assembly; 71. Telescopic component; 72. Sliding block two; 73. Flow guiding port two; 74. Column; 75. Fan wheel; 8. Temperature sensor. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Please see Figure 1-10 The present invention provides a technical solution: a temperature control device for preserving cell culture medium, comprising an insulated box 1, a temperature control back plate 2, a storage mechanism, and a temperature adjustment mechanism. The temperature control back plate 2, the storage mechanism, and the temperature adjustment mechanism are all disposed inside the insulated box 1. The temperature control back plate 2 is fixedly connected to the insulated box 1. The storage mechanism is disposed on one side of the temperature control back plate 2. The temperature adjustment mechanism is disposed on the storage mechanism. The storage mechanism is used to store cell culture medium to be kept at a constant temperature. The temperature adjustment mechanism is used to adjust the preservation temperature of the cell culture medium in the storage mechanism.

[0021] Specifically, such as Figure 1 As shown, the incubator 1 is equipped with a door 11, which is used to open and close the incubator 1, so that the incubator 1 forms a sealed storage condition and the sealed condition is released to facilitate the handling of cell culture medium.

[0022] Specifically, such as Figure 2 and Figure 3 As shown, the storage mechanism includes several partition components 3, several drive components 4, and several storage components 5. The number of partition components 3, drive components 4, and storage components 5 are the same. The drive components 4 and storage components 5 are arranged side by side on top of the partition components 3. The partition components 3 are used to separate the several drive components 4 and the several storage components 5. The drive components 4 are used to automatically pick up and put in the corresponding storage components 5 as a whole. The storage components 5 are used to preserve the cell culture medium at a constant temperature.

[0023] Furthermore, such as Figure 3 As shown, the partition assembly 3 includes a shelf 31 and a partition 32. The partition 32 is fixed on the top of the shelf 31. The drive assembly 4 and the storage assembly 5 are respectively disposed on both sides of the partition 32. The shelf 31 is used to vertically separate a number of drive assemblies 4 and a number of storage assemblies 5. The partition 32 is used to separate the drive assembly 4 and the storage assembly 5 at the top of the shelf 31.

[0024] Furthermore, such as Figure 3 and Figure 10As shown, the driving assembly 4 includes a cylinder 41, an adsorption plate 42, and several suction cups 43. The cylinder 41 is located on one side of the partition plate 32 and is fixed to the top of the shelf plate 31. The adsorption plate 42 is fixed to the output end of the cylinder 41. Several suction cups 43 are fixed on the side of the adsorption plate 42 away from the cylinder 41. Several suction cups 43 are connected to an air extraction device 1 through pipes. The air extraction device 1 is used to extract air, so that the suction cups 43 are in a negative pressure state, which facilitates subsequent adsorption operations. The cylinder 41 is used to drive the adsorption plate 42 and the suction cups 43 on the adsorption plate 42 to move in the direction set by the cylinder 41.

[0025] Furthermore, such as Figures 2-6 and Figure 10 As shown, the storage component 5 includes a storage compartment 51 and a storage plate 52. The storage plate 52 is disposed inside the top of the storage compartment 51. The storage compartment 51 is disposed on the side of the partition plate 32 away from the cylinder 41. The storage plate 52 is used to place cell culture medium. The storage compartment 51 is provided with a return port 53 on its side. The return port 53 is connected to an air extraction device 2. The bottom of the storage compartment 51 near the temperature control back plate 2 is provided with a guide port 54. The storage compartment 51 near the temperature control back plate 2 is provided with several air inlets 55 at equal intervals on its side. Several air inlets 56 are provided at equal intervals along the circumference of the air inlets 55. A connecting plate 57 is fixedly connected to the side of the storage compartment 51 near the cylinder 41. The connecting plate 57 is located near the door 11 and corresponds to the position of the adsorption plate 42.

[0026] Furthermore, such as Figure 6 and Figure 7 As shown, the temperature control back panel 2 is provided with several sets of air outlets 21. The number of sets of air outlets 21 corresponds to the number of storage compartments 51, and the number and position of each set of air outlets 21 correspond to the number and position of air inlets 55. The inner diameter of the air outlet 21 matches the outer diameter of the air inlet 55. A top cover 22 is slidably connected to the air outlet 21. Several air outlet holes 23 are equidistantly arranged on the top cover 22 along the circumference of the top cover 22. A plug 24 is fixedly connected to the side of the top cover 22 facing the air outlet 21. The outer diameter of the plug 24 matches the inner diameter of the air outlet 21. A spring 25 is fitted onto the air outlet 21, and the two ends of the spring 25 are fixedly connected to the inner walls of the top cover 22 and the temperature control back plate 2, respectively.

[0027] In actual operation, in the initial state, the suction device connected to the suction cup 43 is in standby state, the cylinder 41 is in retracted state, that is, the suction cup 43 and the connecting plate 57 are in a non-connected state.

[0028] When staff need to remove or remove cell culture medium, they can manually pull out the storage compartment 51 from between two adjacent sets of vertically adjacent plates 31, or use an electric drive to extend the cylinder 41 corresponding to the storage compartment 51 to be pulled out, causing the adsorption plate 42 to move away from the temperature control back plate 2, so that the suction cup 43 contacts the connecting plate 57, and further drive the storage compartment 51 to move away from the temperature control back plate 2 through the adsorption plate 42 and the suction cup 43, thus achieving the effect of pulling out the storage compartment 51.

[0029] After the staff has finished removing and placing the cell culture medium, if the storage compartment 51 was previously pulled out manually, it can still be pushed manually between the two sets of vertical plates 31. Alternatively, it can be electrically driven. First, the cylinder 41 corresponding to the storage compartment 51 to be pushed in is controlled to extend, so that the suction cup 43 on the adsorption plate 42 contacts the connecting plate 57. Then, the air extraction device is activated to extract air, so that a negative pressure is generated between the suction cup 43 and the connecting plate 57 and the connecting plate 57 is adsorbed. Then, the cylinder 41 is controlled to contract, which moves the adsorption plate 42 towards the temperature control back plate 2. The suction cup 43 on the adsorption plate 42 adsorbed by the connecting plate 57 moves the storage compartment 51 closer to one side of the temperature control back plate 2.

[0030] As the storage compartment 51 moves towards the temperature control back panel 2, the air inlet 55 is initially in a non-contact state with the air outlet 21, then it is embedded in the air outlet 21 and comes into contact with it, and then it comes into contact with the plug 24 on the top cover 22, causing the plug 24 and the top cover 22 to move away from the storage compartment 51, thus completely releasing the blockage from the air outlet 21, until the side of the storage compartment 51 closest to the temperature control back panel 2 is in complete contact with the temperature control back panel 2, at which point the top cover 22 and the temperature control back panel 2 are close to the storage compartment 51. When the inner walls on both sides are at their maximum distance, the spring 25 is stretched, and the interior of the temperature control back plate 2 and the interior of the storage compartment 51 are connected through the air outlet 23, the air inlet 56 and the air inlet 55. At this time, the cold air inside the temperature control back plate 2 can enter the interior of the storage compartment 51 through the air outlet 23, the air inlet 56 and the air inlet 55. At the same time, the second air extraction device is activated to extract the gas inside the storage compartment 51, so that the gas inside the storage compartment 51 is circulated and the temperature uniformity inside the storage compartment 51 is improved. As the storage compartment 51 moves toward the temperature control backplate 2, when the air inlet 55 is not in contact with the plug 24, the spring 25 is in a contracted state. Under the pulling force of the spring 25 when it is contracted, the top cover 22 can cause the plug 24 to embed into the air outlet 21, blocking the air outlet 21. The airflow inside the temperature control backplate 2 can no longer enter the storage compartment 51 through the air outlet 23, air inlet 56, and air inlet 55. Similarly, when the storage compartment 51 moves away from the temperature control backplate 2, the air inlet 55 is released from contact with the plug 24. Under the pulling force of the spring 25 when it is contracted, the top cover 22 can cause the plug 24 to embed into the air outlet 21, blocking the air outlet 21. The airflow inside the temperature control backplate 2 can no longer enter the storage compartment 51 through the air outlet 23, air inlet 56, and air inlet 55, preventing the low-temperature gas inside the temperature control backplate 2 from flowing out, thus achieving the effect of adaptive opening and closing of the air outlet 21 on the temperature control backplate 2.

[0031] Specifically, such as Figure 2 and Figure 8 As shown, the temperature control mechanism includes several flow guiding components 6, several flow splitting components 7, and several temperature sensors 8. The number of flow guiding components 6, flow splitting components 7, and temperature sensors 8 is the same as that of the storage compartments 51, and their positions correspond. The flow guiding components 6, flow splitting components 7, and temperature sensors 8 are all located inside the storage compartments 51. The temperature sensors 8 are fixed on the inner wall of the storage compartments 51. The flow guiding components 6 are used to guide the flow of two or three adjacent sets of storage compartments 51 in the vertical direction. The flow splitting components 7 are used to improve the uniformity of the airflow entering the storage compartments 51 from the temperature control backplate 2, so that the temperature inside the storage compartments 51 is uniform. The temperature sensors 8 are used to detect the temperature inside the storage compartments 51.

[0032] Furthermore, such as Figure 5 , Figure 8 and Figure 9 As shown, the flow guiding assembly 6 includes a flow guiding plate 61, a driving component 63, a push plate 64, a sliding block 65, a spring 66, a top plate 67, and a spring 68. The flow guiding plate 61 is located at the top inside the storage compartment 51 and is rotatably connected to the storage compartment 51. A support 62 is fixedly connected to the side wall of the storage compartment 51. The driving component 63 is fixed on the support 62 and uses a drive motor. The output end of the driving component 63 passes through the support 62 and is connected to the flow guiding plate 61 through a transmission connection. The transmission connection can be a gear meshing connection. The driving component 63 is used to drive the flow guiding plate 61 to swing back and forth alternately around the rotatable connection between the flow guiding plate 61 and the storage compartment 51 through the transmission connection, thereby adjusting the angle of the flow guiding plate 61. A drain port 33 is provided in the vertical direction of the shelf 31. A sliding groove 34 with an approximate U-shape is provided in the horizontal direction at one end of the shelf 31 near the temperature control back plate 2. The drain port 33 is provided through the sliding groove 34. Sliding block 65, spring 66, top plate 67 and spring 68 are all provided in the sliding groove 34. Sliding block 65 is U-shaped and top plate 67 is L-shaped. Push plate 64 is fixedly connected to the bottom of sliding block 65. Top plate 67 is in contact with sliding block 65. Spring 66 is located in the sliding groove 34 at the end away from the temperature control back plate 2. Spring 68 is located in the sliding groove 34 at the end near the temperature control back plate 2. The two ends of spring 66 are fixedly connected to sliding block 65 and shelf 31 respectively. The two ends of spring 68 are fixedly connected to temperature control back plate 2 and top plate 67 respectively. A push groove 58 is provided on the side of the storage compartment 51 near the temperature control back panel 2. The size and position of the push groove 58 match those of the push plate 64. The sliding block 65 has a through-hole 651, and the top plate 67 has a through-hole 671. The through-hole 651, through-hole 671, the drain 33 and the guide port 54 are the same size. When the side wall of the storage compartment 51 near the temperature control back plate 2 is in complete contact with the temperature control back plate 2, the drain 33, through-hole 651 and through-hole 671 are connected and are in the same vertical state as the drain 33.

[0033] It should be noted that if the sliding groove 34 on the shelf 31 does not have the same appearance as the end near the temperature control back panel 2, Figure 8 When the opening shape shown is such that the spring 68 is completely inside the sliding groove 34, the two ends of the spring 68 can be fixedly connected to the shelf 31 and the top plate 67 respectively.

[0034] Furthermore, such as Figure 10 As shown, the diversion assembly 7 includes two sets of telescopic components 71, a second sliding block 72, several columns 74 and several fan wheels 75. The two sets of telescopic components 71 are located on both sides of the second sliding block 72. The two ends of the telescopic components 71 are fixedly connected to the second sliding block 72 and the storage compartment 51 respectively. The second sliding block 72 is slidably connected to the storage compartment 51. The number of air inlets 55 on the columns 74, fan wheels 75 and storage compartment 51 are the same. The column 74 is fixed to the top of the sliding block 72. The spacing between the columns 74 is the same as the spacing between the air inlets 55 on the storage compartment 51. The fan wheel 75 is set on the top side wall of the column 74 and is rotatably connected to the column 74. The sliding block 72 is provided with several guide ports 73. The guide ports 73 are located directly below the fan wheel 75. When in use, the two sets of telescopic components 71 are in a state where one set is extended and the other set is retracted, thereby adjusting the position of the sliding block 72 inside the storage compartment 51 so that the fan wheel 75 corresponds to the cell culture medium or the gap between two adjacent sets of cell culture medium.

[0035] In actual operation, when the staff manually or electrically pushes the storage compartment 51 between the two sets of vertical shelves 31, the storage compartment 51 is initially not in contact with the push plate 64. Then, the storage compartment 51 comes into contact with the push plate 64, and the push plate 64 is located in the pressing groove 58. After that, the storage compartment 51 drives the push plate 64 to move closer to the temperature control back plate 2. Simultaneously, it drives the sliding block 65, which is fixedly connected to the push plate 64, and the top plate 67, which is in contact with the sliding block 65, to move closer to the temperature control back plate 2, causing the spring 66 to be stretched. Spring 368 is compressed until the side of storage compartment 51 closest to the temperature control back plate 2 is completely in contact with the temperature control back plate 2, so that the positions of the drain port 33, the first through port 651, and the second through port 671 are corresponding and in a connected state; if at this time the storage compartment 51 above or below this group of storage compartments is in the same state as this group of storage compartments, and its side closest to the temperature control back plate 2 is completely in contact with the temperature control back plate 2, then the inside of this group of storage compartments 51 can be connected to the storage compartments above or below through the drain port 33, the first through port 651, the second through port 671, and the first guide port 54.

[0036] If it is necessary to disconnect the connection between the drain port 33, the through port 1 651, the through port 2 671 and the guide port 1 54, simply pull the storage compartment 51 out appropriately from between the two sets of shelves 31 in the vertical direction. The distance pulled out is equal to the width of the drain port 33 in the direction of pushing and pulling the storage compartment 51. During this process, under the elastic force of the spring 3 68 in the contracted state and the pulling force of the spring 2 66 in the extended state, the top plate 67, the sliding block 1 65, and the push plate 64 are moved away from the temperature control back plate 2, so that the drain port 33, the through port 1 651, the through port 2 671 and the guide port 1 54 are all in an interlaced state, that is, the connection is disconnected.

[0037] When the storage compartment 51 is fully in contact with the temperature control backplate 2 on the side closest to the temperature control backplate 2, and the temperature control backplate 2 is connected to the air outlet 23, air inlet 56 and air inlet 55 inside the storage compartment 51, by controlling one set of telescopic components 71 to extend and another set of telescopic components 71 to retract, the position of the sliding block 2 72 inside the storage compartment 51 can be adjusted so that the fan wheel 75 corresponds to the cell culture medium or the gap between two adjacent sets of cell culture medium. Since the position of the air inlet 55 on the storage compartment 51 is corresponding, the airflow entering the storage compartment 51 through the air inlet 55 will first impact the fan wheel 75, drive the fan wheel 75 to rotate, and then blow into the storage compartment 51, thereby performing fixed-point rapid cooling or dispersed cooling of the cell culture medium. At the same time, by alternately controlling the extension and retraction of the two sets of telescopic components 71, the position of the sliding block 2 72 and the upper fan wheel 75 can be continuously adjusted, further promoting the dispersion of airflow and uniform temperature inside the storage compartment 51.

[0038] When the storage compartment 51 is fully in contact with the temperature control backplate 2 on the side closest to the temperature control backplate 2, and the temperature control backplate 2 is connected to the air outlet 23, air inlet 56 and air inlet 55 inside the storage compartment 51, the control drive component 63 is activated, driving the guide plate 61 to rotate around the rotational connection between the guide plate 61 and the storage compartment 51. If the guide plate 61 is in a horizontal state, the guide plate 61 does not block the airflow after it has been dispersed by the fan wheel 75, which is the normal state of temperature adjustment inside the storage compartment 51. If the baffle 61 is in a vertical position, and the storage compartments 51 above, below, or below this group of storage compartments 51 are in the same state as this group of storage compartments 51, the cold air entering this group will be guided by the vertical baffle 61 and enter the inlet 33, through-hole 651, through-hole 671, and guide port 54 connected on the upper and lower shelves 31 of this group of storage compartments 51, or enter the lower storage compartment 51, thereby regulating the temperature inside the upper, lower, or lower storage compartments 51; If the distance between the bottom of the guide plate 61 and the temperature control back plate 2 is less than the distance between the top of the guide plate 61 and the temperature control back plate 2, and the storage compartment 51 above the storage compartment 51 is in the same state as the storage compartment 51 in this group, the cold air entering this group will enter the storage compartment 51 through the guide plate 61 and the connecting inlet 33, throughlet 651, throughlet 671, guide inlet 54 and guide inlet 73 at the bottom of the upper storage compartment 51, and enter the upper storage compartment 51 to regulate the temperature inside the upper storage compartment 51. When the airflow from one set of storage compartments 51 is introduced into another set of storage compartments 51 through the inlet 33, the first outlet 651, and the second outlet 671, the airflow will further promote the rotation of the fan wheel 75, thereby further promoting the uniformity of the airflow inside the other set of storage compartments 51.

[0039] It should be noted that the length of the storage compartment 51 in its moving direction is less than the length of the shelf 31; the temperature control back panel 2 is connected to a refrigeration device, and is also equipped with a sensor inside for detecting the temperature of the cold airflow entering the storage compartment 51.

[0040] Operating method of temperature control device for cell culture medium preservation: Step 1: Removing or placing cell culture medium: Manual method: Directly pull the storage compartment 51 out from between two adjacent plates 31; Electric drive method: The control cylinder 41 extends, the suction cup 43 on the adsorption plate 42 contacts the connecting plate 57, the air extraction device makes the suction cup 43 adsorb under negative pressure, and the cylinder 41 continues to extend to pull out the storage compartment 51.

[0041] Step 2: Pushing in and closing storage compartment 51: Push in manually or electrically. During the pushing process, the air inlet 55 first contacts the air outlet 21, then pushes the plug 24 and the top cover 22, the spring 1 25 is stretched, the air outlet 21 opens, and the cold air in the temperature control back plate 2 enters the storage compartment 51 through the air outlet 23 and the air inlet 56; at the same time, the second air extraction device extracts air through the return port 53 to form a circulation. After the storage compartment 51 is fully attached to the temperature control back panel 2, the push plate 64 enters the push groove 58, pushing the sliding block 65 and the top plate 67, causing the spring 66 to stretch and the spring 68 to compress, connecting the drain port 33, the through port 651 and the through port 671.

[0042] Step 3: Temperature sensor 8 detects and temperature control backplane 2 responds: Temperature sensor 8 detects the internal temperature of each storage compartment 51 in real time and provides feedback. The feedback receiving end compares the detected value with the preset storage temperature. If the temperature is too high, the cooling output of the temperature control backplane 2 is activated or increased; if the temperature is too low, the cooling output is reduced.

[0043] Step 4: The flow distribution component 7 adjusts the airflow uniformity: Cold air enters through the air inlet 55 and impacts the fan wheel 75 to make it rotate. The feedback receiving end controls the two sets of telescopic components 71 to extend and retract alternately based on the feedback from the temperature sensor 8 and the temperature distribution. One set extends while the other set retracts. The position of the sliding block 72 is adjusted so that when the fan wheel 75 is aligned with the cell culture medium, the temperature is rapidly reduced at a fixed point; when the fan wheel 75 is aligned with the gap in the culture medium, the temperature is evenly distributed.

[0044] Step 5: The flow guiding component 6 achieves interlayer temperature balance: The drive component 63 controls the angle of the flow guiding plate 61 according to the detection results of the temperature sensor 8. When the flow guiding plate 61 is in a horizontal state, it only adjusts the temperature of its own cell and does not guide the flow to other cells. When the flow guiding plate 61 is in a vertical state, if the temperature of the storage cell 51 above or below deviates, the cold air flows through the inlet 33, the first outlet 651, the second outlet 671, and the first flow guiding port 54 into the adjacent cell to achieve interlayer heat balance. When the flow guiding plate 61 is in an inclined state, if only the storage cell above needs temperature adjustment, the cold air is preferentially guided to the first flow guiding port 54 and the second flow guiding port 73 at the bottom of the upper cell.

[0045] Step Six: Continuous Circulation and Stabilization: The air extraction device 2 continuously circulates air to prevent local temperature stratification. The temperature sensor 8 monitors in real time and the feedback receiving end dynamically adjusts the output of the temperature control backplate 2, the position of the telescopic component 71, and the angle of the guide plate 61 until the temperature in all storage compartments 51 stabilizes within the preset range.

[0046] The above methods enable high-precision, high-uniformity, and rapid-response temperature control, ensuring the stable temperature of the cell culture medium.

[0047] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0048] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A temperature control device for preserving cell culture medium, comprising an incubator (1), a temperature control backplate (2), a storage mechanism, and a temperature adjustment mechanism, characterized in that, The temperature control backplate (2), storage mechanism, and temperature adjustment mechanism are all installed inside the insulation box (1). The temperature control backplate (2) is fixedly connected to the insulation box (1). The storage mechanism is installed on one side of the temperature control backplate (2). The storage mechanism includes several partition components (3), several drive components (4), and several storage components (5). The temperature adjustment mechanism includes several flow guiding components (6), several flow splitting components (7), and several temperature sensors (8). The number of partition components (3), drive components (4), storage components (5), flow guiding components (6), flow splitting components (7), and temperature sensors (8) are the same and their positions correspond. The temperature sensors (8) are fixed on the inner wall of the storage compartment (51). The separating component (3) includes a shelf (31) and a partition plate (32). The storage component (5) includes a storage compartment (51) and a storage plate (52). The flow guiding component (6) includes a flow guiding plate (61), a driving component (63), a push plate (64), a sliding block (65), a spring (66), a top plate (67), and a spring (68). The flow guiding plate (61) is located at the top inside the storage compartment (51). The shelf (31) has a flow inlet (33) in the vertical direction. The shelf (31) has a sliding groove (34) in the horizontal direction at one end near the temperature control back plate (2). The flow inlet (33) passes through the sliding groove (34). The push plate (64) is fixedly connected to the bottom of the sliding block (65). The top plate (67) is in contact with the sliding block (65). The spring (66) and the spring (68) are located at the two ends of the sliding groove (34).

2. The temperature control device for preserving cell culture medium according to claim 1, characterized in that, The partition plate (32) is fixed on the top of the shelf (31), and the drive assembly (4) and the storage assembly (5) are disposed on the top of the shelf (31) and respectively disposed on both sides of the partition plate (32).

3. The temperature control device for preserving cell culture medium according to claim 2, characterized in that, The drive assembly (4) includes a cylinder (41), an adsorption plate (42), and several suction cups (43). The cylinder (41) is located on one side of the partition plate (32) and fixed to the top of the shelf plate (31). The adsorption plate (42) is fixed to the output end of the cylinder (41). Several suction cups (43) are fixed on the side of the adsorption plate (42) away from the cylinder (41). Several suction cups (43) are connected to a suction device.

4. The temperature control device for preserving cell culture medium according to claim 3, characterized in that, The storage plate (52) is located at the top inside the storage compartment (51), and the storage compartment (51) is located on the side of the partition plate (32) away from the cylinder (41); The storage compartment (51) is provided with a return port (53) on its side. The return port (53) is connected to a second air extraction device. The bottom of the storage compartment (51) near the temperature control back plate (2) is provided with a guide port (54). The storage compartment (51) near the temperature control back plate (2) is provided with several air inlets (55) at equal intervals on its side. Several air inlets (56) are provided at equal intervals along the circumferential direction of the air inlets (55). A connecting plate (57) is fixedly connected to the side of the storage compartment (51) near the cylinder (41). The connecting plate (57) is located near the door (11), and the position of the connecting plate (57) corresponds to that of the adsorption plate (42).

5. A temperature control device for preserving cell culture medium according to claim 4, characterized in that, The temperature control backplate (2) is provided with several sets of air outlets (21). The number of sets of air outlets (21) corresponds to the number of storage compartments (51). The number and position of each set of air outlets (21) correspond to the number and position of air inlets (55). The inner diameter of the air outlet (21) matches the outer diameter of the air inlet (55). A top cover (22) is slidably connected to the air outlet (21). Several air outlet holes (23) are equidistantly arranged on the top cover (22) along the circumferential direction of the top cover (22). A plug (24) is fixedly connected to the side of the top cover (22) facing the air outlet (21). The outer diameter of the plug (24) matches the inner diameter of the air outlet (21). A spring (25) is fitted on the air outlet (21), and the two ends of the spring (25) are fixedly connected to the inner wall of the top cover (22) and the temperature control back plate (2), respectively.

6. A temperature control device for preserving cell culture medium according to claim 5, characterized in that, The guide plate (61) is rotatably connected to the storage compartment (51). A support (62) is fixedly connected to the side wall of the storage compartment (51). The driving component (63) is fixed on the support (62). The output end of the driving component (63) passes through the support (62) and is connected to the guide plate (61) in a transmission manner.

7. A temperature control device for preserving cell culture medium according to claim 6, characterized in that, The first sliding block (65), the second spring (66), the top plate (67) and the third spring (68) are all arranged in the sliding groove (34). The first sliding block (65) is U-shaped, the top plate (67) is L-shaped, the two ends of the second spring (66) are fixedly connected to the first sliding block (65) and the shelf (31) respectively, and the two ends of the third spring (68) are fixedly connected to the temperature control back plate (2) and the top plate (67) respectively. The storage compartment (51) has a push groove (58) on the side near the temperature control back plate (2), and the push groove (58) matches the size and position of the push plate (64).

8. A temperature control device for preserving cell culture medium according to claim 7, characterized in that, The sliding block (65) has an opening (651), and the top plate (67) has an opening (671). The openings (651), (671), (33), and (54) are of the same size.

9. A temperature control device for preserving cell culture medium according to claim 8, characterized in that, The diversion component (7) includes two sets of telescopic components (71), a second sliding block (72), several columns (74) and several fan wheels (75). The two sets of telescopic components (71) are located on both sides of the second sliding block (72). The two ends of the telescopic components (71) are fixedly connected to the second sliding block (72) and the storage compartment (51) respectively. The second sliding block (72) is slidably connected to the storage compartment (51). The number of air inlets (55) on the columns (74), fan wheels (75) and storage compartment (51) is the same.

10. A temperature control device for preserving cell culture medium according to claim 9, characterized in that, The column (74) is fixed on the top of the sliding block (72). The spacing between the columns (74) is the same as the spacing between the air inlets (55) on the storage compartment (51). The fan wheel (75) is set on the top side wall of the column (74). The fan wheel (75) is rotatably connected to the column (74). The sliding block (72) has several guide ports (73) which are located directly below the fan wheel (75).

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