A potassium ion complex of panax notoginseng polysaccharide and a preparation method and application thereof

Abstract

The application discloses a potassium ion complex of panax notoginseng polysaccharide and a preparation method and application thereof, and belongs to the technical field of natural product chemistry and medicine. The complex is formed by coordination of panax notoginseng polysaccharide and potassium ions, wherein the content of potassium ions is 70-100 mg / g, the complex has a triple helix structure, and the micro-morphology is in a spherical and reticular aggregate form. The monosaccharide composition mainly includes glucose, galactose, galacturonic acid, arabinose and rhamnose. The complex shows a significant potassium ion slow-release characteristic under a simulated gastrointestinal fluid condition. The complex can be used for preparing a slow-release potassium supplement medicine, health care product or food, and simultaneously has an antioxidant activity. The technical scheme provided by the application overcomes the defects of a large stimulation and uncontrollable release of an existing oral potassium preparation, realizes the unity of stable release of potassium ions and additional biological activity, and provides a new way for high-value utilization of panax notoginseng processing by-products.

Description

Technical Field

[0001] This invention relates to the fields of natural product chemistry and pharmaceutical technology, specifically to a potassium ion complex of Panax notoginseng polysaccharide, its preparation method, and its application. Background Technology

[0002] Sanqi ( Panax notoginseng Panax notoginseng (Burk.) FH Chen is a perennial herb belonging to the genus Panax in the family Araliaceae. It is a traditional and precious Chinese medicinal herb. Panax notoginseng has a long history of medicinal use, traditionally used for dispersing blood stasis, stopping bleeding, reducing swelling, and relieving pain. Modern pharmacological studies have shown that Panax notoginseng contains various active ingredients such as saponins, polysaccharides, flavonoids, and amino acids. Panax notoginseng polysaccharides are a type of water-soluble polysaccharide extracted from the roots, rhizomes, stems, leaves, flowers, or processed residues of Panax notoginseng, and are among the most abundant active ingredients in Panax notoginseng. Existing literature reports that Panax notoginseng polysaccharides possess various biological activities, including immunomodulation, antitumor, antioxidant, anti-inflammatory, and hypoglycemic effects. However, current utilization of Panax notoginseng polysaccharides mainly focuses on their direct extraction as raw materials or primary health products; there are no reports of chelating Panax notoginseng polysaccharides with metal ions (especially potassium ions) to prepare functional polysaccharide-metal complexes. As a natural polymer, Panax notoginseng polysaccharide contains abundant functional groups such as hydroxyl (-OH) and carboxyl (-COOH) groups on its molecular chain, which has a natural structural basis for forming coordination bonds with metal ions and has the potential to serve as a metal ion delivery carrier.

[0003] Potassium is an essential macroelement for the human body, crucial for maintaining osmotic pressure balance inside and outside cells, neuromuscular excitability, and normal cardiac function. Normal serum potassium concentration ranges from 3.5 to 5.5 mmol / L; a serum potassium concentration below 3.5 mmol / L is considered hypokalemia. Common causes of hypokalemia include long-term use of diuretics (such as furosemide and hydrochlorothiazide), gastrointestinal loss due to vomiting and diarrhea, malnutrition, chronic kidney disease, and certain endocrine disorders (such as primary aldosteronism). Clinical manifestations include muscle weakness, fatigue, and arrhythmias; severe cases can be life-threatening due to respiratory muscle paralysis or malignant arrhythmias. Currently, most commercially available potassium supplements are primarily inorganic potassium salts, which have drawbacks such as rapid release and easy gastrointestinal irritation. Therefore, developing a novel potassium supplement that effectively replenishes potassium ions, possesses good gastrointestinal tolerance and controllable release characteristics, and also exhibits additional biological activity is a pressing technical challenge in the field of potassium supplementation therapy. Summary of the Invention

[0004] The technical problem that this invention aims to solve is: (1) To address the problems of existing oral potassium chloride preparations such as high gastrointestinal irritation, uncontrollable potassium ion release rate, and lack of additional biological activity, a novel potassium supplement material with natural polysaccharides as carriers, sustained-release properties, and antioxidant activity is provided.

[0005] (2) To address the lack of Panax notoginseng polysaccharide-potassium ion complexes in the existing technology, a Panax notoginseng polysaccharide potassium ion complex with high potassium ion loading, well-defined structure and controllable performance and its preparation method are provided.

[0006] (3) Expand the high-value utilization of processing by-products such as Panax notoginseng residue and provide a technical solution to convert Panax notoginseng polysaccharide into functional potassium supplement and antioxidant materials.

[0007] To solve the above technical problems, the present invention provides the following technical solution: In a first aspect, a potassium ion complex of Panax notoginseng polysaccharide is provided, which is formed by the complexation of Panax notoginseng polysaccharide and potassium ions through coordination bonds, wherein the potassium ion content is 70-100 mg / g, and the complex has a triple helix structure and exhibits a spherical and network aggregated morphology.

[0008] The complex comprises glucose (Glc), galactose (Gal), galacturonic acid (Gal-UA), arabinose (Ara), and rhamnose (Rha), with the following molar percentages: glucose 55%–57%, galactose 22%–24%, galacturonic acid 8%–10%, arabinose 9%–11%, and rhamnose 2%–3%. More preferably, the molar percentages of the monosaccharides are: glucose 56.11%, galactose 22.76%, galacturonic acid 8.88%, arabinose 9.99%, and rhamnose 2.26%.

[0009] The complex exhibits the characteristic of slow-release potassium ions under simulated gastrointestinal fluid conditions, with a cumulative potassium ion release rate of 41.96% to 42.65% over 24 hours in simulated intestinal fluid at pH 6.8.

[0010] Secondly, a method for preparing the aforementioned Panax notoginseng polysaccharide potassium ion complex is provided, comprising the following steps: Dissolve Panax notoginseng polysaccharide in water, add potassium salt, adjust pH to 7-9, shake to react, precipitate with alcohol, centrifuge, wash, collect the precipitate, and dry to obtain Panax notoginseng polysaccharide potassium ion complex.

[0011] Preferably, the potassium salt is potassium chloride (KCl), and the mass ratio of Panax notoginseng polysaccharide to potassium salt is 1:1 to 1.5.

[0012] Preferably, the shaking reaction time is 1.5 to 2.5 hours and the temperature is 55 to 75°C; the alcohol precipitation is performed by adding 4 to 5 times the volume of anhydrous ethanol and allowing it to stand at 0 to 4°C for 10 to 14 hours to precipitate.

[0013] The Panax notoginseng polysaccharides are derived from one or more of the main root, fibrous roots, or residue of Panax notoginseng.

[0014] Preferably, the Panax notoginseng polysaccharide is prepared by the following method: Panax notoginseng residue is decocted with water 1 to 3 times, the extracts are combined, concentrated and then ethanol is added, the mixture is allowed to stand for alcohol precipitation, the precipitate is collected and dried to obtain Panax notoginseng polysaccharide.

[0015] More preferably, the mass-to-volume ratio of the Panax notoginseng residue to water is 1:6-10, in g / mL; the concentration is to concentrate to 0.1-0.15 times the original extract volume; and the amount of ethanol added is 3-4 times the volume of the concentrated liquid.

[0016] The Panax notoginseng residue is the residue obtained from the main root of Panax notoginseng through the extraction process of Panax notoginseng saponins.

[0017] Thirdly, the uses of the aforementioned Panax notoginseng polysaccharide potassium ion complex are provided: (i) Use in the preparation of pharmaceuticals, health products, or food for potassium supplementation. Preferably, the complex slowly releases potassium ions in the gastrointestinal tract, serving as a sustained-release potassium supplement.

[0018] (ii) Application in the preparation of antioxidant drugs, health products or food.

[0019] Compared with the prior art, the Panax notoginseng polysaccharide potassium ion complex of the present invention has the following significant beneficial effects: (1) High potassium ion loading: The potassium ion content in the complex of this invention reaches 80-100 mg / g, which is much higher than the metal loading level of conventional polysaccharide-metal complexes. The high loading means that in potassium supplementation, only a small dose of the complex is needed to meet the daily potassium supplementation requirements, thus improving the convenience of medication.

[0020] (2) Excellent sustained-release properties: The complex of this invention exhibits significant sustained-release behavior under simulated gastrointestinal fluid conditions. In simulated intestinal fluid at pH 6.8, the cumulative release rate of potassium ions over 24 hours is only 35%–43%, while the release rate of potassium chloride control under the same conditions can reach over 70%. The sustained-release properties can: (a) avoid local irritation of the gastrointestinal mucosa by high concentrations of potassium ions, reducing the incidence of adverse gastrointestinal reactions; (b) maintain stable blood potassium concentration, reduce "peak-trough" fluctuations, and reduce the risk of hyperkalemia; (c) reduce the frequency of administration and improve patient compliance.

[0021] (3) Preservation of the natural triple helix structure: One of the key innovations of this invention is that the natural triple helix structure of Panax notoginseng polysaccharide is not destroyed during the complexation reaction. The triple helix conformation is an important structural basis for polysaccharides to exert biological activities such as immunomodulation and antioxidation. The preservation of this conformation means that the complex of this invention can not only deliver potassium ions, but also exert the biological activity of Panax notoginseng polysaccharide itself, thus achieving the dual function of "potassium supplementation + biological activity".

[0022] (4) Unique microstructure: The microstructure of the complex of the present invention exhibits a spherical and network aggregated form, which is beneficial to: (a) increasing the specific surface area and providing a physical barrier for the slow release of potassium ions; (b) having good flowability and compressibility during formulation processing.

[0023] (5) Clear structural characterization: The present invention accurately determined the monosaccharide composition of the complex and clarified the molar percentage range of Glc:Gal:Gal-UA:Ara:Rha (55~57:22~24:8~10:9~11:2~3), providing a scientific basis for product quality control.

[0024] (6) Simple and environmentally friendly preparation process: This invention uses water as the reaction medium, potassium chloride as the potassium source, and ethanol as the precipitant. The entire preparation process does not use toxic organic solvents, which meets the requirements of green chemistry and clean production. The preparation conditions are mild (55-75℃), the operation is simple, and it is easy to scale up to industrial production.

[0025] (7) The raw materials are widely available and inexpensive: Panax notoginseng polysaccharides can be extracted from the processing residue of Panax notoginseng, which is an industrial waste after the extraction of saponins from Panax notoginseng. The source is abundant and the cost is extremely low. This invention provides a new way for the high-value utilization of Panax notoginseng residue, which has both economic and environmental benefits.

[0026] (8) It also has antioxidant activity: Panax notoginseng polysaccharide itself has the ability to scavenge hydroxyl radicals. After complexing with potassium ions, its antioxidant activity is retained or even enhanced (which may be related to the stabilizing effect of potassium ions on the conformation of polysaccharide chains). This allows the complex of the present invention to be applied to the fields of antioxidant functional foods or health products, thus expanding the scope of application. Attached Figure Description

[0027] Figure 1 Photographs of the appearance of freeze-dried powders of Panax notoginseng polysaccharide (A) and Panax notoginseng polysaccharide potassium ion complex (B); Figure 2 The molecular weight distribution of Panax notoginseng polysaccharide (A) and Panax notoginseng polysaccharide potassium ion complex (B); Figure 3 IR spectra of Panax notoginseng polysaccharide and its potassium ion complex; Figure 4 XRD results of Panax notoginseng polysaccharide and Panax notoginseng polysaccharide potassium ion complex; Figure 5 DSC curves of Panax notoginseng polysaccharide and its potassium ion complex; Figure 6 UV spectra of Panax notoginseng polysaccharide and its potassium ion complex; Figure 7 The experimental results for Panax notoginseng polysaccharide and its potassium ion complex Congo red; Figure 8 SEM images of Panax notoginseng polysaccharide and its potassium ion complex. Detailed Implementation

[0028] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments, but this does not limit the present invention in any way. Any modifications or improvements made based on the teachings of the present invention shall fall within the protection scope of the present invention.

[0029] The processes, conditions, reagents, and experimental methods used in implementing this invention, except as specifically mentioned below, are all common knowledge and general knowledge in the field, and this invention does not have any particular limitations. Experimental methods in the embodiments that do not specify specific conditions are generally performed under conventional conditions or as recommended by the manufacturer.

[0030] Unless otherwise stated, all technical terms and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. However, in the event of any conflict, the specification containing the definitions shall prevail.

[0031] The following is information on the source and specifications of the main raw materials and reagents used in the examples: Table 1. Information on the sources of experimental materials and reagents

[0032] Example 1: Preparation of Panax notoginseng polysaccharides Weigh 1000 g of Panax notoginseng residue and add purified water at a material-to-liquid ratio of 1:10 (w / v). Extract in a constant temperature water bath at 100℃ for 6 h, filter, and collect the filtrate. Add ultrapure water to the filter residue at a material-to-liquid ratio of 1:6 (w / v) and extract in a boiling water bath for 6 h, then filter. Combine the filtrates from both extractions and centrifuge at 8000 r / min for 5 min. Collect the supernatant and discard the precipitate. Rotate the supernatant under reduced pressure at 80℃ to 1 / 10 of its original volume. Then, slowly add 4 times the volume of anhydrous ethanol to the concentrated solution while stirring, and allow it to stand at 4℃ for 12 h for alcohol precipitation. Collect the precipitate after filtration, freeze-dry, and pulverize to obtain Panax notoginseng polysaccharide (CP00).

[0033] Example 2 Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1 was weighed and dissolved in 50 mL of ultrapure water. Then, 750 mg of potassium chloride was added (the mass ratio of Panax notoginseng polysaccharide to potassium chloride was 1:1.5). The pH of the reaction was adjusted to 9 with 2 mol / L NaOH, and the mixture was placed in a constant temperature shaker at 65°C and 120 rpm for 1.5 h. After the reaction was complete, the mixture was precipitated with 4 times the volume of anhydrous ethanol, allowed to stand at 4°C for 12 h, centrifuged to remove the supernatant, and then washed three times with anhydrous ethanol. Finally, the precipitate was freeze-dried and pulverized to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK). ICP-MS analysis showed a potassium ion content of 99.77 mg / g and a CPK yield of 80.24%.

[0034] Using Panax notoginseng polysaccharide (CP00) as a control, the appearance of the potassium ion complex of Panax notoginseng polysaccharide (CPK) after complexation with potassium chloride differs. CP00 is pale yellow, while CPK is even paler, appearing grayish-white (see...). Figure 1 ).

[0035] Example 3: Structural Characterization of the Seven-Polysaccharide Potassium Ion Complex 1. Determination of monosaccharide composition Monosaccharide components were analyzed using a Thermo ICS 5000+ ion chromatography system (Thermo Fisher Scientific, USA). Each monosaccharide standard was prepared into a 10 mg / mL stock solution, which was then diluted to prepare a series of standard solutions. Accurately weigh the Panax notoginseng polysaccharide potassium ion complex sample (CPK) prepared in Example 2 and the Panax notoginseng polysaccharide sample (CP00) prepared in Example 1, add 1 mL of 2 mol / L TFA solution, and hydrolyze at 121℃ for 2 h. After drying the hydrolysate under nitrogen, wash with 99.99% methanol to remove residual acid, and finally dissolve in sterile water to obtain the test solution.

[0036] The chromatographic conditions were set as follows: a Dionex™ CarboPac™ PA20 column (150 mm × 3.0 mm, 10 μm) was used, with ultrapure water as phase A, 0.1 mol / L NaOH as phase B, and a mixture of 0.1 mol / L NaOH and 0.2 mol / L NaAc as phase C. The flow rate was 0.5 mL / min, and the column temperature was 30℃. The gradient elution program was as follows: 0–26 min, A:B:C gradient changed from 95:5:0 to 85:5:10; 26–42 min, maintained at 85:5:10; 42.1–52 min, gradient changed from 60:0:40 to 60:40:0; 52.1–60 min, switched to 95:5:0 for column equilibration. The injection volume was 5 μL. Data acquisition and processing were performed using Chromeleon software.

[0037] Qualitative analysis was performed based on the retention time of the target compound, combined with an electrochemical detector. Quantitative analysis was conducted using the external standard method. A standard curve was plotted with the mass concentration of a series of standard solutions on the x-axis and the corresponding peak area on the y-axis, and the content of the analyte in the sample was calculated based on the standard curve.

[0038] 2. Determination of relative molecular weight by HPGPC method Chromatographic conditions and system suitability test: Two PL-aquagel-OH gel chromatography columns (7.5 mm × 50 mm, 8 μm and 7.5 mm × 300 mm, 8 μm) were used in series, with 0.1 mol / L NaNO3 solution as the mobile phase. The column temperature was set at 45℃, the flow rate at 1 mL / min, and the injection volume at 20 μL. Experimental data were analyzed using Agilent Open LabCDS software. The molecular weight of the Panax notoginseng polysaccharide to be tested was calculated by comparing the peak times of polyethylene glycol (PEG) standards with different molecular weights (106, 194, 610, 1470, 3880, 15190, 30320, 73850, 128800, 427500, 1044000, 1511000 Da).

[0039] The relative molecular weight of the polysaccharides was determined by high-performance gel permeation chromatography (HPGPC). An appropriate amount of NaNO3 was accurately weighed, dissolved in purified water, and diluted to 1 L to prepare a 0.1 mol / L NaNO3 solution. This solution was then degassed by sonication for 30 min and used as the mobile phase. 3-5 mg of the Panax notoginseng polysaccharide potassium ion complex sample (CPK) prepared in Example 2 and the Panax notoginseng polysaccharide sample (CP00) prepared in Example 1 were weighed and placed in 2 mL sample vials. 1 mL of the above NaNO3 solution was added, and the samples were dissolved at room temperature for 1-5 h (the dissolution time could be extended appropriately depending on the sample dissolution). After complete dissolution, the samples were filtered through a 0.45 μm PTFE membrane and then injected for analysis.

[0040] 3. IR Measurement Accurately weigh 1 mg each of the Panax notoginseng polysaccharide potassium ion complex sample (CPK) prepared in Example 2 and the Panax notoginseng polysaccharide sample (CP00) prepared in Example 1, mix them with 100 mg of KBr powder, grind them evenly in an agate mortar, and then press them into transparent thin sheets. Fourier transform infrared spectroscopy was used to analyze the samples at 4000–400 cm⁻¹. - ¹Scanning measurements were performed within the wavenumber range.

[0041] 4. XRD Measurement The potassium ion complex sample of Panax notoginseng polysaccharide (CPK) prepared in Example 2 and the Panax notoginseng polysaccharide sample (CP00) prepared in Example 1 were taken and evenly spread on a glass slide and fixed on the sample stage. The measurement conditions were as follows: a Cu-Kα ray source (λ = 1.5406 Å) was used, the tube voltage was 40 kV, the tube current was 40 mA; the scanning range 2θ was 5° to 80°, and the scanning rate was 5 ° / min. The entire experiment was conducted at an ambient temperature of (25 ± 1) °C and a relative humidity of (50 ± 5)% to ensure the stability of the test conditions.

[0042] 5. DSC Measurement Thermal analysis was performed using DSC on the Panax notoginseng polysaccharide potassium ion complex sample (CPK) prepared in Example 2 and the Panax notoginseng polysaccharide sample (CP00) prepared in Example 1. The determination was conducted under a nitrogen atmosphere, using an empty crucible as a reference. The temperature program was set as follows: initial temperature 30 °C, increased to 230 °C at a rate of 10 °C / min. Data acquisition began after the temperature stabilized at the initial temperature.

[0043] 6. UV Measurement Solutions of Panax notoginseng polysaccharide potassium ion complex (CPK) prepared in Example 2 and Panax notoginseng polysaccharide sample (CP00) prepared in Example 1 (1 mg / mL) were prepared separately, with purified water as a blank control. The samples were scanned in the wavelength range of 200–800 nm using a UV-Vis spectrophotometer.

[0044] 7. Congo Red Experiment The conformation of the polysaccharides was analyzed using the Congo red method. A 0.2 mmol / L Congo red solution and NaOH solutions with gradient concentrations of 0-0.5 mol / L (0, 0.1, 0.2, 0.3, 0.4, 0.5 mol / L) were prepared. 1 mL each of the Panax notoginseng polysaccharide potassium ion complex sample (CPK) prepared in Example 2 and the Panax notoginseng polysaccharide sample (CP00) prepared in Example 1 (2 mg / mL) were mixed with 1 mL of Congo red solution, and then 2 mL of NaOH solution of different concentrations were added. The mixtures were reacted at room temperature in the dark for 10 min. The corresponding solutions without added polysaccharides were used as blank controls. The maximum absorption wavelength of each reaction system was measured within the wavelength range of 300–700 nm.

[0045] 8. SEM Measurement The Panax notoginseng polysaccharide potassium ion complex sample (CPK) prepared in Example 2 and the Panax notoginseng polysaccharide sample (CP00) prepared in Example 1 were uniformly dispersed and directly adhered to conductive adhesive. After gold sputtering, they were observed by scanning electron microscopy at an accelerating voltage of 1 kV, and finally obtained polysaccharide microstructure images with resolutions of 2, 5 and 10 μm.

[0046] Experimental results: (1) Monosaccharide composition The monosaccharide composition of the Panax notoginseng polysaccharide potassium ion complex sample (CPK) prepared in Example 2 and the Panax notoginseng polysaccharide sample (CP00) prepared in Example 1 is shown in Table 2. The molar ratio of monosaccharide composition in the Panax notoginseng polysaccharide potassium ion complex sample has changed.

[0047] Table 2. Molar percentage of monosaccharide composition of CP00 and CPK

[0048] (2) As shown in Figure 2, the molecular weight distribution of the potassium ion complex of Panax notoginseng polysaccharide differs from that of Panax notoginseng polysaccharide. Panax notoginseng polysaccharide shows a distinct double peak, while the potassium ion complex of Panax notoginseng polysaccharide shows one single peak and one shoulder peak. The Mw, Mn and PDI of the potassium ion complex of Panax notoginseng polysaccharide are all lower than those of Panax notoginseng polysaccharide.

[0049] (3) IR measurement results like Figure 3As shown, the FT-IR spectra of the potassium ion complex of Panax notoginseng polysaccharide and Panax notoginseng polysaccharide are at 3402.11 and 3450.7, 2928.87 and 2937.72, 1614.78 and 1631.69, and 1409.85 cm⁻¹. 1 The peaks exhibited are typical polysaccharide absorption peaks, belonging to the stretching vibrations of OH, CH, CC, and CO. Panax notoginseng polysaccharides and K... + After the ionic reaction, most of the characteristic peaks of the two compounds were similar, indicating that the functional groups of Panax notoginseng polysaccharide did not change after the metal ion complexation process. In the figure, the characteristic band of -OH in the potassium ion complex of Panax notoginseng polysaccharide showed a slight red shift (from 3450.7 cm⁻¹). 1 up to 3402.11 cm 1 ).

[0050] (4) XRD measurement results like Figure 4 As shown, both the potassium ion complex of Panax notoginseng polysaccharide and Panax notoginseng polysaccharide exhibit broad diffraction peaks characteristic of polysaccharides. Compared with Panax notoginseng polysaccharide, the peak intensity of the potassium ion complex of Panax notoginseng polysaccharide is stronger, which may be due to the K + Ion complexation affects peak absorption intensity. The overall difference is small, indicating that K... + The complexation of ions did not change the amorphous state of the polysaccharide.

[0051] (5) DSC measurement results like Figure 5 As shown in Table 3, the differential scanning calorimetry (DSC) curves of the potassium ion complex of Panax notoginseng polysaccharide show that, compared with that of Panax notoginseng polysaccharide, the onset and termination temperatures of the DSC peaks are both lower. The H change was relatively small, and the starting temperature of the potassium ion complex of Panax notoginseng polysaccharide was 53.80℃, which was significantly lower than that of Panax notoginseng polysaccharide (70.29℃).

[0052] Table 3. DSC thermal analysis parameters of CP00 and CPK

[0053] (6) UV measurement results like Figure 6 As shown, neither the potassium ion complex of Panax notoginseng polysaccharide nor Panax notoginseng polysaccharide showed obvious absorption peaks in the 260-280 nm range, indicating that Panax notoginseng polysaccharide and potassium ion complex exhibited significant absorption peaks. + No nucleic acids or proteins were produced during the ion complexation process.

[0054] (7) Results of the Congo Red Experiment like Figure 7As shown, the potassium ion complex of Panax notoginseng polysaccharide exhibits a red shift in λmax in Congo red solution, consistent with the properties of Panax notoginseng polysaccharide itself. However, with increasing NaOH concentration, λmax shows a blue shift. Therefore, it can be confirmed that the potassium ion complex of Panax notoginseng polysaccharide possesses a triple helix structure, indicating that Panax notoginseng polysaccharide and K... + Its triple helix structure was not destroyed after ion complexation.

[0055] (8) SEM measurement results like Figure 8 As shown, both the potassium ion complex of Panax notoginseng polysaccharide and Panax notoginseng polysaccharide exhibit spherical and network aggregate morphologies, indicating that the overall microstructure of Panax notoginseng polysaccharide did not change significantly after complexing with K+ ions.

[0056] Example 4 Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) Weigh 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1, dissolve it in 50 mL of ultrapure water, and then add 750 mg of potassium chloride (the mass ratio of Panax notoginseng polysaccharide to potassium chloride is 1:1.5). Adjust the pH of the reaction to 9 with 2 mol / L NaOH, and then place it in a constant temperature shaker at 75°C and 120 rpm for 2 h. After the reaction is complete, precipitate it with 4 times the volume of anhydrous ethanol, let it stand at 4°C for 12 h, centrifuge and discard the supernatant, wash the precipitate three times with anhydrous ethanol, and finally freeze-dry and pulverize it to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK) with a potassium ion content of 75.30 mg / g.

[0057] Example 5 Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) Weigh 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1, dissolve it in 50 mL of ultrapure water, and then add 500 mg of potassium chloride (the mass ratio of Panax notoginseng polysaccharide to potassium chloride is 1:1). Adjust the pH of the reaction to 7 with 2 mol / L NaOH, and then place it in a constant temperature shaker at 55°C and 120 rpm for 1.5 h. After the reaction is complete, precipitate it with 4 times the volume of anhydrous ethanol, let it stand at 4°C for 12 h, centrifuge and discard the supernatant, wash the precipitate three times with anhydrous ethanol, and finally freeze-dry and pulverize it to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK) with a potassium ion content of 73.40 mg / g.

[0058] Example 6 Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1 was weighed and dissolved in 50 mL of ultrapure water. Then, 625 mg of potassium chloride was added (the mass ratio of Panax notoginseng polysaccharide to potassium chloride was 1:1.25). The pH of the reaction was adjusted to 7 with 2 mol / L NaOH, and the mixture was placed in a constant-temperature shaker at 65°C and 120 rpm for 2 hours. After the reaction was complete, the mixture was precipitated with 4 times the volume of anhydrous ethanol, allowed to stand at 4°C for 12 hours, centrifuged to remove the supernatant, and then washed three times with anhydrous ethanol. Finally, the precipitate was freeze-dried and pulverized to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK), with a potassium ion content of 76.18 mg / g.

[0059] Example 7 Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) Weigh 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1, dissolve it in 50 mL of ultrapure water, and then add 750 mg of potassium chloride (the mass ratio of Panax notoginseng polysaccharide to potassium chloride is 1:1.5). Adjust the pH of the reaction to 7 with 2 mol / L NaOH, and then place it in a constant temperature shaker at 75°C and 120 rpm for 2.5 h. After the reaction is complete, precipitate it with 4 times the volume of anhydrous ethanol, let it stand at 4°C for 12 h, centrifuge and discard the supernatant, wash the precipitate three times with anhydrous ethanol, and finally freeze-dry and pulverize it to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK) with a potassium ion content of 77.61 mg / g.

[0060] Example 8 Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) Weigh 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1, dissolve it in 50 mL of ultrapure water, and then add 750 mg of potassium chloride (the mass ratio of Panax notoginseng polysaccharide to potassium chloride is 1:1.5). Adjust the pH of the reaction to 8 with 2 mol / L NaOH, and then place it in a constant temperature shaker at 55°C and 120 rpm for 2 h. After the reaction is complete, precipitate it with 4 times the volume of anhydrous ethanol, let it stand at 4°C for 12 h, centrifuge and discard the supernatant, wash the precipitate three times with anhydrous ethanol, and finally freeze-dry and pulverize it to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK) with a potassium ion content of 81.02 mg / g.

[0061] Example 9: Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) Weigh 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1, dissolve it in 50 mL of ultrapure water, and then add 500 mg of potassium chloride (the mass ratio of Panax notoginseng polysaccharide to potassium chloride is 1:1). Adjust the pH of the reaction to 8 with 2 mol / L NaOH, and then place it in a constant temperature shaker at 65°C and 120 rpm for 2.5 h. After the reaction is complete, precipitate it with 4 times the volume of anhydrous ethanol, let it stand at 4°C for 12 h, centrifuge and discard the supernatant, wash the precipitate three times with anhydrous ethanol, and finally freeze-dry and pulverize it to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK) with a potassium ion content of 77.07 mg / g.

[0062] Example 10 Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) Weigh 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1, dissolve it in 50 mL of ultrapure water, and then add 625 mg of potassium chloride (the mass ratio of Panax notoginseng polysaccharide to potassium chloride is 1:1.25). Adjust the pH of the reaction to 8 with 2 mol / L NaOH, and then place it in a constant temperature shaker at 75°C and 120 rpm for 1.5 h. After the reaction is complete, precipitate it with 4 times the volume of anhydrous ethanol, let it stand at 4°C for 12 h, centrifuge and discard the supernatant, wash the precipitate three times with anhydrous ethanol, and finally freeze-dry and pulverize it to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK) with a potassium ion content of 79.45 mg / g.

[0063] Example 11 Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1 was weighed and dissolved in 50 mL of ultrapure water. Then, 625 mg of potassium chloride was added (the mass ratio of Panax notoginseng polysaccharide to potassium chloride was 1:1.25). The pH of the reaction was adjusted to 9 with 2 mol / L NaOH, and the mixture was placed in a constant-temperature shaker at 55°C and 120 rpm for 2.5 h. After the reaction was complete, the mixture was precipitated with 4 times the volume of anhydrous ethanol, allowed to stand at 4°C for 12 h, centrifuged to remove the supernatant, and then washed three times with anhydrous ethanol. Finally, the precipitate was freeze-dried and pulverized to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK), with a potassium ion content of 83.26 mg / g.

[0064] Example 12 Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) Weigh 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1, dissolve it in 50 mL of ultrapure water, and then add 750 mg of potassium chloride (the mass ratio of Panax notoginseng polysaccharide to potassium chloride is 1:1.5). Adjust the pH of the reaction to 9 with 2 mol / L NaOH, and then place it in a constant temperature shaker at 65°C and 120 rpm for 1.5 h. After the reaction is complete, precipitate it with 4 times the volume of anhydrous ethanol, let it stand at 4°C for 12 h, centrifuge and discard the supernatant, wash the precipitate three times with anhydrous ethanol, and finally freeze-dry and pulverize it to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK) with a potassium ion content of 84.75 mg / g.

[0065] Example 13 Preparation of Panax notoginseng polysaccharide potassium ion complex (CPK) Weigh 500 mg of Panax notoginseng polysaccharide (CP00) prepared in Example 1, dissolve it in 50 mL of ultrapure water, and then add 500 mg of potassium chloride (the mass ratio of Panax notoginseng polysaccharide to potassium chloride is 1:1). Adjust the pH of the reaction to 9 with 2 mol / L NaOH, and then place it in a constant temperature shaker at 75°C and 120 rpm for 2 h. After the reaction is complete, precipitate it with 4 times the volume of anhydrous ethanol, let it stand at 4°C for 12 h, centrifuge and discard the supernatant, wash the precipitate three times with anhydrous ethanol, and finally freeze-dry and pulverize it to obtain the Panax notoginseng polysaccharide potassium ion complex (CPK) with a potassium ion content of 82.58 mg / g.

[0066] Example 14: Determination of in vitro potassium ion release characteristics of Panax notoginseng polysaccharide potassium ion complex Prepare gastrointestinal fluids with different pH values ​​(Reference: Wang Zhineng, Wang Yun, Wu Guangxu, Wu Qinghua, Su Dongxiao, Li Li, Yang Hualin, Chen Sha. Effects of in vitro simulated gastrointestinal digestion on the release of phenolic substances and antioxidant activity in lotus leaves under different heat treatments [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(10): 1975-1982.). Weigh 6 g of NaH2PO4 and dissolve it in 250 mL of ultrapure water. Add 190 mL of 0.2 mol / L NaOH, and then slowly add 400 mL of ultrapure water. Stir for 5 min, and adjust the pH to 4.5±0.02, 6.8±0.02 and 7.4±0.02 with 2 mol / L NaOH or HCl. Transfer to a 1000 mL volumetric flask and make up to volume. Add 7 mL of HCl to 900 mL of ultrapure water, then add 2 g of NaCl. Adjust the pH of the solution to 1.2 ± 0.02 with 6 mol / L HCl. Transfer the solution to a 1000 mL volumetric flask, make up to volume, and let stand for 1-2 h.

[0067] 200 mg of CPK was dissolved in 10 mL of simulated gastrointestinal fluid at different pH values. The solution was placed in a dialysis bag (DW5000). 30 mL of the same solution was measured from outside the dialysis bag. The simulated in vitro release device was placed in a constant-temperature shaker at 37°C and 150 rpm. Samples were taken at 0 min, 5 min, 10 min, 20 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, and 24 h. After each sampling, the same volume of simulated gastrointestinal fluid at different pH values ​​was added. Potassium chloride was used as a positive control, and the procedure was repeated. The potassium ion concentration in the sample was determined using an automated electrolyte analyzer, and Kc was calculated. + Cumulative ion release rate = K + Cumulative release of ions (external K) + (Ions + Cumulative Sampling Take-Away Amount) / Total K + Ion quantity × 100%. The release model was fitted using Origin 2024 software.

[0068] The results are shown in Tables 4 and 6. The results indicate that the potassium ion complex of Panax notoginseng polysaccharide and potassium chloride were initially released rapidly in different pH media, followed by a slower release over time. At 24 h, the cumulative potassium ion release rates of the Panax notoginseng polysaccharide potassium ion complex and potassium chloride at pH 6.8 were 41.96% and 70.15%, respectively. Due to experimental design limitations, the cumulative potassium chloride release rate at 24 h did not reach 100%. This is because the total volume in the experimental system was 40 mL, with 10 mL inside the dialysis bag and 30 mL outside. At equilibrium, the K+ in the external solution... + The ion ratio equals the external volume divided by the total volume. Some potassium ions will still remain inside the dialysis bag. Therefore, the theoretical upper limit is not 100%. The experimental procedures and calculation methods for both the Panax notoginseng polysaccharide potassium ion complex and potassium chloride were exactly the same, making this experimental comparison reasonable. Since the release rate of the Panax notoginseng polysaccharide potassium ion complex is significantly lower than that of potassium chloride, and it did not reach 50% release rate even after 24 hours, this indicates that the Panax notoginseng polysaccharide potassium ion complex releases K... + The ions are released slowly and continuously. Based on the simulation results and parameters of the in vitro release kinetic model of the Panax notoginseng polysaccharide potassium ion complex and potassium chloride (Tables 4 and 7), the optimal release models for the Panax notoginseng polysaccharide potassium ion complex and potassium chloride are the first-order kinetic model and the Ritger-Peppas kinetic model (R²>0.9), indicating that K... + The ion release mechanism is Fickian diffusion, which means that K + The release of ions is primarily driven by the concentration gradient, with diffusion controlling K. +Ion release rate. The potassium ion complex of Panax notoginseng polysaccharide releases slowly and incompletely, with a relatively rapid release in the early stages, but limited by matrix diffusion, resulting in almost no release in the later stages. The maximum release rate is approximately 35.98-41.59%, consistent with a sustained-release phenomenon. KCl releases more rapidly and completely, with a maximum release rate of approximately 67.20-81.92%, showing no sustained-release characteristics. The release rate is relatively uniform, but diffusion control leads to early K ion release... + The cumulative release of ions approached equilibrium. Both substances exhibited Fickian diffusion, but their release behaviors differed: potassium chloride diffused rapidly and released in high amounts, while the potassium polysaccharide diffused slowly and released in low amounts. This verifies the sustained-release properties of the polysaccharide, and the K+ ion complex of the Panax notoginseng polysaccharide potassium ion complex... + Ion release is limited by matrix diffusion, while potassium chloride, as a free ion, diffuses more efficiently. The K+ of potassium chloride... + Ion release is primarily limited by diffusion within the dialysis bag. The potassium ion complex of Panax notoginseng polysaccharide... + Ion release is diffusion primarily restricted by both the polysaccharide matrix and the dialysis bag; therefore, the Panax notoginseng polysaccharide potassium ion complex exhibits slow release of K+. + Ionic characteristics.

[0069] Table 4. Cumulative potassium ion release rate of CPK in simulated gastrointestinal fluids at different pH values ​​from 0 to 24 h.

[0070] Table 5 Simulation results and parameters of CPK in vitro release kinetics model

[0071] Table 6. Cumulative potassium ion release rate of KCl in simulated gastrointestinal fluids at different pH values ​​from 0 to 24 h

[0072] Table 7 Simulation results and parameters of KCl in vitro release kinetic model

[0073] Example 15 In vitro OH radical scavenging experiment of Panax notoginseng polysaccharide potassium ion complex Sample solution preparation: The samples of Panax notoginseng polysaccharide prepared in Example 1 and the potassium ion complex of Panax notoginseng polysaccharide prepared in Example 2 were accurately measured and prepared with purified water to prepare sample solutions with concentrations of 0.25, 0.5, 1.0, 2.0, 4.0 and 8.0 mg / mL.

[0074] Positive control solution: Take VC and prepare solutions with purified water at concentrations of 0.25, 0.5, 1.0, 2.0, 4.0, and 8.0 mg / mL.

[0075] The reaction system consisted of 0.1 mL hydrogen peroxide solution (9 mmol / L), 1 mL ferrous sulfate solution (9 mmol / L), 1 mL salicylic acid-ethanol solution (9 mmol / L), 2 mL deionized water, and 1 mL sample solutions of different concentrations. The mixture was incubated in a 37 ℃ water bath for 30 min, with vitamin C as a positive control, and the absorbance was measured at 510 nm. The hydroxyl radical scavenging rate was calculated using the following formula: ; In the formula, A0 is the control solution, A1 is the absorbance of the sample in the reaction system, and A2 is the absorbance blank of the sample.

[0076] The experimental results are shown in Table 6. Both Panax notoginseng polysaccharide and its potassium ion complex exhibited potent and concentration-dependent hydroxyl radical scavenging activity. With increasing sample concentration, the corresponding hydroxyl radical scavenging activity and scavenging rate gradually increased. The in vitro hydroxyl radical scavenging activity of the potassium ion complex was significantly higher than that of Panax notoginseng polysaccharide, suggesting that K... + Ion complexation optimizes the spatial structure of Panax notoginseng polysaccharide to enhance its antioxidant activity.

[0077] Table 6. In vitro OH radical scavenging rates of CP00 and CPK ( (n=3)

[0078] Note: * indicates a statistically significant difference between CPK and CP00. P <0.05,** P <0.01, *** P <0.001, **** P <0.0001.

[0079] Example 16: Optimization and Screening of Preparation Process Conditions (1) pH value Using Panax notoginseng polysaccharide (CP00) as raw material, 500 mg of CP00 was dissolved in 50 mL of ultrapure water, and then potassium chloride in a 0.75 ratio of CP00 was added. The pH of the reaction was adjusted to 4, 5, 6, 7, 8, 9, and 10 with 2 mol / L NaOH or HCl. The mixture was then placed in a constant-temperature shaker at 65℃, 120 rpm, and 3 h. After the reaction, the mixture was precipitated with 4 times the volume of anhydrous ethanol, allowed to stand at 4℃ for 12 h, centrifuged to discard the supernatant, and then washed three times with anhydrous ethanol. Finally, the precipitate was freeze-dried and pulverized to obtain CPK. The potassium content in CPK was determined by ICP-MS. + The content of ions, expressed in terms of K + Ion content was used as an evaluation index to examine the effect of pH on complexation reactions.

[0080] The results are shown in Table 9. The results indicate that the complexation efficiency of Panax notoginseng polysaccharide potassium complex increases with increasing pH, reaching its lowest efficiency at pH 4, and highest at pH 10. + The highest ion content was 62.8±1.21 mg / g, indicating the best complexation effect. Considering that Panax notoginseng polysaccharides are easily hydrolyzed in a strongly alkaline environment, the optimal pH is 7-9.

[0081] Table 9. Potassium ion content of Panax notoginseng polysaccharide potassium ion complex under different pH conditions ( (n=3)

[0082] (2) Temperature 500 mg of CP00 was dissolved in 50 mL of ultrapure water, and then potassium chloride (0.75 ratio of CP00) was added. The pH of the reaction mixture was adjusted to 6 with 2 mol / L NaOH or HCl, and then placed in a constant-temperature shaker. The temperature was set at 45℃, 55℃, 65℃, 75℃, and 85℃, the rotation speed was 120 rpm, and the reaction time was 3 h. After the reaction was completed, the mixture was precipitated with 4 times the volume of anhydrous ethanol, allowed to stand at 4℃ for 12 h, centrifuged to discard the supernatant, and then washed three times with anhydrous ethanol. Finally, the precipitate was freeze-dried and pulverized to obtain CPK. The potassium content in CPK was determined by ICP-MS. + The content of ions, expressed in terms of K + Ion content was used as an evaluation index to examine the effect of temperature on the complexation reaction.

[0083] The results are shown in Table 10. At 75℃, the potassium ion complex in Panax notoginseng polysaccharide contained K... + The highest ion content was 49.79±1.33 mg / g, showing the best complexation effect. At a temperature of 85℃, K... + The lowest ion content was 47.57±1.28 mg / g, indicating the worst complexation effect. When the reaction system temperature was too low, the thermal motion rate between ions decreased, reducing the probability of collisions and hindering the interaction between polysaccharides and potassium. + Ions are difficult to approach effectively, making complexation reactions difficult to proceed; while excessively high temperatures may cause some of the potassium ion complexes of Panax notoginseng polysaccharides to decompose or undergo side reactions, thereby reducing the potassium content in the complex. + The ion content decreases. Therefore, the preferred reaction temperature is 55℃~75℃, and more preferably 65℃.

[0084] Table 10 Potassium ion content of Panax notoginseng polysaccharide potassium ion complex under different temperature conditions ( (n=3)

[0085] (3) Time 500 mg of CP00 was dissolved in 50 mL of ultrapure water, and then potassium chloride (0.75 ratio of CP00) was added. The pH of the reaction mixture was adjusted to 6 with 2 mol / L NaOH or HCl, and then placed in a constant-temperature shaker at 65 °C and 120 rpm for 1, 1.5, 2, 2.5, 3, 3.5, and 4 h. After the reaction, the mixture was precipitated with 4 times the volume of anhydrous ethanol, allowed to stand at 4 °C for 12 h, centrifuged to remove the supernatant, and then washed three times with anhydrous ethanol. Finally, the precipitate was freeze-dried and pulverized to obtain CPK. The K+ ion content in CPK was determined by ICP-MS, and the effect of time on the complexation reaction was investigated using the K+ ion content as an evaluation index.

[0086] The results are shown in Table 11. When the reaction time between Panax notoginseng polysaccharide and potassium chloride was 2 h, the K+ of the potassium ion complex of Panax notoginseng polysaccharide was... + The highest ion content was 55.25±1.22 mg / g, indicating the best complexation effect. With prolonged reaction time, the potassium ion content of the Panax notoginseng polysaccharide complex increased. + The ion content exhibited unstable increases and decreases. This phenomenon may be because the reaction was relatively complete at 2 hours, thus K... + The ion complexation efficiency is the highest. When the reaction time is short, the polysaccharide and K... + Insufficient ion complexation leads to instability in the product formed, affecting the K in the complex. + The ion content is low; excessively long reaction time may lead to a decrease in the stability of the complex system, K + The ions do not bind tightly to Panax notoginseng polysaccharides, resulting in K in the complex being... + The ion content is unstable. Therefore, the optimal reaction time is 1.5 h to 2.5 h.

[0087] Table 11 Potassium ion content of Panax notoginseng polysaccharide potassium ion complex under different time conditions ( (n=3)

[0088] (4) Ratio of polysaccharide to potassium chloride 500 mg of CP00 was dissolved in 50 mL of ultrapure water. Potassium chloride was added in CP00 solutions at ratios of 0.25, 0.5, 0.75, 1, 1.25, 1.5, and 1.75. The pH of the reaction mixture was adjusted to 6 with 2 mol / L NaOH or HCl, and the mixture was placed in a constant-temperature shaker at 65°C and 120 rpm for 3 h. After the reaction, the mixture was precipitated with 4 times the volume of anhydrous ethanol, allowed to stand at 4°C for 12 h, centrifuged to remove the supernatant, and then washed three times with anhydrous ethanol. Finally, the precipitate was freeze-dried and pulverized to obtain CPK. The potassium content in CPK was determined by ICP-MS. +The content of ions, expressed in terms of K + Ion content was used as an evaluation index to investigate the effect of the ratio of polysaccharide to potassium chloride on the complexation reaction.

[0089] The results are shown in Table 12. When the ratio of Panax notoginseng polysaccharide to potassium chloride is 1 / 1.25, the potassium chloride content in the Panax notoginseng polysaccharide potassium ion complex is... + The highest ion content was 62.02±1.45 mg / g, indicating the best complexation effect. When the potassium chloride ratio was low, the K in the solution... + The number of ions is limited, and they cannot fully bind to all the effective binding sites on the polysaccharide chain. In the formed complex, each Panax notoginseng polysaccharide molecule binds to K... + The number of ions did not reach its maximum theoretical capacity, therefore the measured total K + The ion content is low. When the proportion of potassium chloride is too high, the ionic strength of the system may increase, causing the polysaccharide chains to react with K+. + The weakening of adsorption between ions reduces the stability of the potassium ion complex of Panax notoginseng polysaccharide, thus partially binding K. + Ions are easily released. Therefore, the preferred ratio of Panax notoginseng polysaccharide to potassium chloride is 1:1 to 1.5.

[0090] Table 12 Potassium ion content of Panax notoginseng polysaccharide potassium ion complex under different potassium chloride ratios ( (n=3)

[0091] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A potassium ion complex of Panax notoginseng polysaccharide, characterized in that, It is formed by the complexation of Panax notoginseng polysaccharide and potassium ions through coordination bonds. The potassium ion content in the Panax notoginseng polysaccharide potassium ion complex is 70-100 mg / g, and the complex has a triple helix structure. Its microscopic morphology is spherical and network aggregated.

2. The Panax notoginseng polysaccharide potassium ion complex according to claim 1, characterized in that, The monosaccharide composition of the Panax notoginseng polysaccharide potassium ion complex includes glucose, galactose, galacturonic acid, arabinose and rhamnose; the molar percentage of its monosaccharide composition is: glucose 55% to 57%, galactose 22% to 24%, galacturonic acid 8% to 10%, arabinose 9% to 11%, and rhamnose 2% to 3%.

3. The Panax notoginseng polysaccharide potassium ion complex according to claim 1, characterized in that, The potassium ion complex of Panax notoginseng polysaccharide has the characteristic of slow-release potassium ions under simulated gastrointestinal fluid conditions. The cumulative potassium ion release rate in simulated intestinal fluid at pH 6.8 is 41.96% to 42.65% over 24 hours.

4. A method for preparing the Panax notoginseng polysaccharide potassium ion complex according to any one of claims 1-3, characterized in that, Includes the following steps: After dissolving Panax notoginseng polysaccharide in water, potassium salt was added and the pH was adjusted to 7-9; the reaction was shaken, followed by alcohol precipitation, centrifugation, washing, collection of the precipitate, and drying to obtain the Panax notoginseng polysaccharide potassium ion complex.

5. The method according to claim 4, characterized in that, The potassium salt is potassium chloride, and the mass ratio of Panax notoginseng polysaccharide to potassium salt is 1:1 to 1.

5.

6. The method according to claim 4, characterized in that, The shaking reaction time is 1.5–2.5 h, and the temperature is 55–75 °C; the alcohol precipitation is carried out by adding 4–5 times the volume of anhydrous ethanol and allowing it to stand at 0–4 °C for 10–14 h to precipitate.

7. The method according to claim 4, characterized in that, The Panax notoginseng polysaccharide is prepared by the following method: Panax notoginseng residue is decocted with water 1 to 3 times, the extracts are combined, concentrated and then ethanol is added, the mixture is allowed to stand for alcohol precipitation, the precipitate is collected and dried to obtain Panax notoginseng polysaccharide.

8. The method according to claim 7, characterized in that, The mass-to-volume ratio of the Panax notoginseng residue to water is 1:6-10, with units of g / mL; the concentration is to concentrate to 0.1-0.15 times the original extract volume; the amount of ethanol added is 3-4 times the volume of the concentrated liquid.

9. The use of the Panax notoginseng polysaccharide potassium ion complex according to any one of claims 1-3 or the Panax notoginseng polysaccharide potassium ion complex prepared by the method according to any one of claims 4-8 in the preparation of drugs, health products or food for supplementing potassium ions; Preferably, the complex slowly releases potassium ions in the gastrointestinal tract and is used as a sustained-release potassium supplement.

10. The use of the Panax notoginseng polysaccharide potassium ion complex according to any one of claims 1-3 or the Panax notoginseng polysaccharide potassium ion complex prepared by the method according to any one of claims 4-8 in the preparation of antioxidant drugs, health products or food.

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