Optical detection of microbial culture vessels and methods of manufacture

Abstract

The application provides an optical detection microorganism culture container and a manufacturing method, relates to the field of microorganism detection, and aims at the problem that the formed detection channel size consistency cannot be guaranteed due to the fact that the glass material is used in the online detection culture container at present. The application uses transparent engineering plastic material to integrally injection-mold a detection area, so that the opposite two side walls for limiting the detection light path are directly formed by the injection-mold cavity with high rigidity, compared with the uncontrollability of fluid tension in the traditional glass blowing process, the structure converts the internal fluid width of the optical channel into the engineering size constrained by the injection mold, reduces the light path tolerance between different batches of containers at the source, so that the batch-produced culture containers can still maintain high detection reference consistency without optical calibration one by one, and the system stability and data reliability of the online optical detection are improved.

Description

Technical Field

[0001] This invention relates to the field of microbial detection, and more specifically to an optical microbial culture container and its manufacturing method. Background Technology

[0002] In the field of online optical detection for microbial culture, existing culture containers mostly use conical flasks formed by traditional glass blowing processes. In terms of space and structure, existing technologies typically have the bottom of the glass culture flask concave inwards or convex outwards to form a local channel for the light beam to pass through. In terms of workflow, the light source and receiver of the external detection device are respectively arranged on both sides of this channel. The detection light beam passes through the culture medium within the channel, and the system calculates the optical density (OD value) of the culture medium based on the attenuation of the transmitted light intensity, thereby achieving continuous online monitoring of the bacterial concentration.

[0003] Because existing technologies employ a one-piece bottom channel structure based on inorganic glass blowing, they are prone to conflict with the dimensional consistency requirements of high-precision optical inspection during actual manufacturing and testing. The rheological properties, surface tension, and localized cooling shrinkage rate of the glass melt during blowing are difficult to precisely constrain. When attempting to form stepped or deep-cavity channels of varying widths at the bottom of the bottle, the wall thickness distribution of the glass material at corners and deep cavities tends to fluctuate randomly, leading to discrete deviations in the width of the solidified channel cavity. During optical inspection, random errors in channel width cause the actual optical path of the beam through the culture medium to deviate from the theoretical design value, resulting in distorted absorbance data. To correct for optical interference caused by manufacturing defects, existing inspection processes typically require individual calibration and optical coefficient adjustment for each glass culture bottle, increasing system operational complexity. Summary of the Invention

[0004] The purpose of this invention is to address the shortcomings of existing technologies by providing an optical detection microbial culture container and its manufacturing method. This solves the problem that the glass blowing process in the prior art cannot guarantee the consistency of the dimensions of multiple optical path detection channels, resulting in limited optical detection accuracy and the need for cumbersome post-calibration.

[0005] The first objective of this invention is to provide an optical detection container for microbial culture, which employs the following solution: It includes the bottle body and the bottle bottom, with a detection area on the bottle bottom for the detection beam to pass through; The detection area is provided with at least two sets of detection channels of different widths, and the detection channels are formed by a recessed structure toward the inside of the container and / or a protruding structure toward the outside of the container; The detection channel forms a fluid space inside the container to hold the culture medium. The distance between the two opposite side walls of the detection channel along the direction through which the detection beam passes is the detection optical path. The detection area is integrally molded from transparent engineering plastic, and the detection beam penetrates the side wall of the detection channel and the culture medium filling it.

[0006] Furthermore, the at least two sets of detection channels are collinearly distributed, and the direction of the collinear distribution extends radially or chordally along the bottom of the bottle; when there are three or more sets of detection channels, each set of detection channels is arranged sequentially according to the size of its detection optical path, so that the sidewall of the concave structure or convex structure has a stepped structure.

[0007] Furthermore, the bottle body and bottle bottom are separately formed components, and the edge of the bottle bottom is sealed and fixed to the edge of the bottle body by ultrasonic welding, hot melt welding, adhesive bonding, threaded connection or snap-fit.

[0008] Furthermore, the container includes a left half-shell and a right half-shell that are formed independently, and the left half-shell and the right half-shell are spliced ​​and fixed together, with the splice seam between the left half-shell and the right half-shell located outside the detection area.

[0009] A second objective of this invention is to provide a method for manufacturing an optically detectable microbial culture container, for manufacturing the optically detectable microbial culture container as provided in the first objective, comprising: Transparent engineering plastic raw materials are injected into an injection mold with corresponding cavities for injection molding; At least two sets of recessed structures and / or raised structures of different widths are formed in one step in the detection area of ​​the bottle bottom through the cavity of the injection mold, so that the sidewalls of the recessed structures and / or raised structures are formed into a continuous and closed solid structure, which serves as the detection area. After cooling and solidification, the mold is opened and demolded to obtain a completely transparent container or independent parts that can be assembled to form a container.

[0010] A third objective of this invention is to provide an optical detection microbial culture container, employing the following solution: It includes the bottle body and the bottle bottom, with a detection area on the bottle bottom for the detection beam to pass through; The detection area is provided with at least two sets of detection channels of different widths, and the detection channels are formed by a recessed structure toward the inside of the container and / or a protruding structure toward the outside of the container; The detection channel forms a fluid space inside the container to hold the culture medium. The distance between the two opposite side walls of the detection channel along the direction through which the detection beam passes is the detection optical path. The bottom of the bottle is made of opaque material, and the detection channel has through holes on the side walls. A transparent lens is sealed and embedded in the through hole, and the transparent lens forms an optical window for the detection beam to pass through the culture medium.

[0011] Furthermore, at least two sets of detection channels are collinearly distributed, with the direction of collinear distribution extending radially or chordally along the bottom of the bottle; when there are three or more sets of detection channels, each set of detection channels is arranged sequentially according to the size of its detection optical path, so that the sidewall of the concave or convex structure has a stepped structure.

[0012] Furthermore, the bottle body and bottle bottom are separately formed components. The bottle bottom is formed by machining, and the edge of the bottle bottom is sealed and fixed to the edge of the bottle body by ultrasonic welding, hot melt welding, adhesive bonding, threaded connection or snap-fit.

[0013] A fourth objective of this invention is to provide a method for manufacturing an optically detectable microbial culture container, for manufacturing the optically detectable microbial culture container as provided in the first objective, comprising: The bottle bottom is formed using an opaque material, and at least two sets of recessed and / or raised structures of different widths are formed on the bottle bottom. Through holes are made on the opposite side walls of the recessed structure and / or the raised structure, and the through holes penetrate the side walls along the direction of the detection beam; A transparent lens is embedded and sealed within the through hole to form an optical window.

[0014] Furthermore, in the step of creating the through hole, the through hole is created by drilling after the bottle bottom has been formed. In the step of embedding and sealing the transparent lens in the through hole, the transparent lens is placed in the mold and integrally injection molded with the opaque material using an insert injection molding process, or an adhesive is used to bond and fix the molded transparent lens in the through hole.

[0015] Compared with the prior art, the advantages and positive effects of this invention are: To address the issue of inconsistent detection channel dimensions in current online detection systems using glass culture containers, this invention utilizes a transparent engineering plastic material for integral injection molding of the detection area. This allows the opposing side walls defining the detection optical path to be directly solidified from a highly rigid injection mold cavity. Compared to the uncontrollable fluid tension inherent in traditional glass blowing processes, this structure transforms the internal fluid width of the optical channel into an engineering dimension constrained by the injection mold. This reduces the optical path tolerance between different batches of containers at the source, enabling mass-produced culture containers to maintain high consistency in detection standards without individual optical calibration. This improves the system stability and data reliability of online optical detection. Attached Figure Description

[0016] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0017] Figure 1 This is a schematic diagram of the overall structure of an optical detection microbial culture container in one or more embodiments of the present invention.

[0018] Figure 2 This is a schematic cross-sectional view of an optical detection microbial culture container in one or more embodiments of the present invention.

[0019] Figure 3 This is a schematic diagram of the overall structure of an opaque container in one or more embodiments of the present invention.

[0020] Figure 4 This is a cross-sectional structural diagram of an opaque container in one or more embodiments of the present invention.

[0021] Among them, 1. Bottle body; 2. Bottle bottom; 3. Detection area; 4. First detection channel; 5. Second detection channel; 6. First sidewall; 7. Second sidewall; 8. Fluid space; 9. Detection channel; 10. Optical window. Detailed Implementation

[0022] Example 1 In a typical embodiment of the present invention, such as Figure 1 , Figure 2 As shown, an optical detection microbial culture container is presented.

[0023] In this embodiment, the optical detection channel 9 is precisely constructed by molding a fully transparent polymer material. The optical detection microbial culture container includes a bottle body 1 and a bottle bottom 2, and the bottle bottom 2 is provided with a detection area 3 through which the detection beam passes.

[0024] The detection area 3 has at least two sets of detection channels 9 of different widths. The detection channels 9 are formed by recessed structures pointing inwards into the container and / or protruding structures pointing outwards. The interior of each detection channel 9 forms a fluid space 8 communicating with the interior of the container to contain the culture medium. The distance between the opposite side walls of each detection channel 9 along the direction of the detection beam is the detection optical path. The detection area 3 is integrally molded from transparent engineering plastic, allowing the detection beam to directly penetrate the side walls of the detection channels 9 and the culture medium filling them.

[0025] In this embodiment, the optical detection microbial culture container is designed to be compatible with conventional constant temperature shaking incubators and shakers. The bottle body 1 is generally a conical rotating structure with a gradually decreasing cross-sectional area from bottom to top. The fluid space 8 within the detection channel 9 is connected to the main cavity of the container. During actual culture operation, the liquid culture medium and suspended bacteria inside the container will naturally flow into and fill the fluid space 8.

[0026] The detection channel 9, as the core structure for containing the culture medium and allowing the light beam to penetrate, can be shaped by two structural forms or a combination thereof, in order to adapt to different overall equipment requirements and molding constraints.

[0027] When a protruding structure is used to extend outward from the container, a hollow auxiliary cavity is formed below the reference plane of the bottle bottom 2, extending downward or outward. During cultivation, the liquid naturally flows into and fills the protruding cavity under the influence of gravity. The light source and receiver of the external detection mechanism can be directly clamped on both sides of the protruding structure for horizontal through-beam detection without encroaching on the effective main culture volume inside the container. Furthermore, the movement trajectory of the external optical sensor is entirely below the base plane of the container, and there is no spatial interference with the tapered outer wall of the main bottle body 1.

[0028] When a recessed structure is adopted, the bottom 2 of the bottle is partially recessed into the main cavity of the container, forming a sunken liquid-containing groove or cavity inside. The culture medium fills the relatively low-lying recessed space, and the light beam penetrates this area through the bottom perforation or the included angle of the side wall. Hiding and protecting the optically transparent sidewalls with specific optical paths within the overall outer contour of the container reduces the risk of scratches on the optical surfaces due to external impacts during routine handling or high-speed shaking on a shaker. Simultaneously, it allows the bottom of the container to maintain a relatively flat outer edge support boundary, facilitating stable placement on conventional experimental surfaces.

[0029] In some designs targeting wide measurement ranges, protruding structures facing outwards and recessed structures facing inwards can be reused. To achieve multiple optical paths with a large span within the limited projected area of ​​the bottle bottom 2, such as a first detection channel 4 containing a 1mm optical path and a second detection channel 5 containing a 10mm optical path, the narrower optical path channel can be designed to be recessed inwards, while the wider optical path channel can be designed to be protruding outwards. Through Z-axis height misalignment and spatial folding, not only is the arrangement space for multiple channels expanded, but the volume shrinkage rate during injection molding of the entire bottle bottom 2 is also effectively balanced, resulting in a more uniform overall wall thickness distribution of the melt inside the mold.

[0030] like Figure 2 As shown, the detection channel 9 on one side of the fluid space 8 has a first sidewall 6 on one side and a second sidewall 7 on the other side. Since the light beam passes through the first sidewall 6, the culture medium whose thickness is limited by the two sidewalls, and the second sidewall 7 in sequence, the distance between the sidewalls determines the effective path length for the scattering and absorption of photons with the bacteria.

[0031] In this embodiment, the transparent engineering plastic material can be made of materials such as polycarbonate (PC) or polymethyl methacrylate (PMMA). Compared with traditional inorganic silicate glass, it has a lower melt viscosity and exhibits better cavity fitting ability under mold pressure. The solid continuous sidewall structure of the detection area 3 through which the detection beam passes eliminates the risk of capillary leakage caused by assembly gaps, maintaining the sterile boundary required for microbial culture.

[0032] To ensure the linearity and accuracy of optical detection, the first and second sidewalls 6 and 7 of the detection channel 9, which allow the detection beam to enter and exit, adopt a parallel planar structure when defining the detection optical path. This parallel sidewall structure ensures that the detection beam, when incident perpendicularly, will not experience additional refraction or beam divergence due to the interface tilt angle, thus guaranteeing precise alignment of the beam's exit trajectory with the external receiving sensor.

[0033] Furthermore, at the junctions of the recessed or raised structures within the detection channel 9, rounded or chamfered corners are smoothly provided. These chamfered corners not only eliminate geometric stress concentration during injection molding and improve the structural yield strength at the bottom, but also reduce stagnation in the culture medium at deeper levels of the channel from a hydrodynamic perspective. During shaker operation, the chamfered surfaces help guide the liquid to form smooth eddies, preventing abnormal sedimentation of bacteria in corners and ensuring uniformity of bacterial concentration within the light-transmitting area.

[0034] like Figure 1 As shown, in the spatial layout of multiple detection channels 9, at least two sets of detection channels 9 are collinearly distributed, and the direction of the collinear distribution extends radially or chordally along the bottom of the bottle 2. The linear array structure allows the external optical transceiver components to be aligned sequentially with detection channels 9 of different widths simply by translating along a one-dimensional linear guide, reducing the motion freedom requirements of the detection mechanism.

[0035] When there are three or more detection channels 9, each group of detection channels 9 is arranged sequentially according to the length of its detection optical path, so that the sidewalls of the concave or convex structures have a stepped structure. The introduction of the stepped structure not only facilitates space avoidance of the core inside the injection mold, but also helps the culture medium to form appropriate local turbulence along the stepped surface under oscillation, increasing the fluid mixing degree, so that the bacterial concentration in the detection channel 9 is closer to the macroscopic average concentration of the container body.

[0036] In this embodiment, the spacing between detection channels 9 of different widths can be 1 mm, 2 mm, 5 mm, or 10 mm. Different spacings are chosen for different detection channels 9. When the culture medium is in the lag phase of the early stage of culture, due to the low bacterial concentration (e.g., OD600 below 0.5), if a narrow channel of 1 mm is used, the attenuation of the light beam after passing through is small, and the small changes of the photoelectric sensor are easily drowned out by the system's electrical noise. At this time, the external system can be aligned with the wide channel of 10 mm for detection, amplifying the light attenuation signal by increasing the travel distance of photons in the fluid. Conversely, when the bacteria enter the logarithmic or stationary phase and the concentration rises rapidly (e.g., OD600 exceeds 20), the transmitted light intensity in the 10 mm channel will attenuate. At this time, the system will switch to a short optical path channel of 1 mm or 2 mm to ensure that enough transmitted photons can reach the receiving end. Through the cascading of specific size parameters, the container can cover the detection needs of the entire concentration range of the microbial life cycle.

[0037] Considering the complex, multi-path stepped channels integrated at the bottom of the culture container, interference could easily occur if the entire container were injection molded and demolded. Therefore, an adaptive separation was implemented in the assembly structure: the body 1 and the bottom 2 are independently molded components. The edges of the bottom 2 are sealed and fixed to the edges of the body 1 using ultrasonic welding, thermoforming welding, adhesive bonding, threaded connections, or snap-fit ​​methods. For example, the high-frequency vibration of ultrasonic welding causes diffusion cross-linking of polymer macromolecular segments, achieving fluid sealing without introducing third-party chemical adhesives.

[0038] When using threaded connections, the outer side of the bottom end of the bottle body 1 and the inner side of the bottle bottom 2 are respectively formed with interlocking threads, and a medical-grade silicone O-ring is pressed between the mating end faces. The bottom of the container is detachable, which facilitates the individual replacement or sampling of the test base under certain special culture processes. If a snap-fit ​​method is used, the elastic deformation of the polymer material itself is utilized, and the pressing during assembly is achieved by setting inverted claws and annular positioning grooves on the edge. Combined with the interference design of the end face, the sealing standard for preventing leakage can also be met.

[0039] In other alternative embodiments, the container includes a left half-shell and a right half-shell, which are separately molded and fixed together, with the seam between the two halves located outside the detection area 3. The longitudinal segmentation logic makes the bottom recessed / protruding structure appear semi-open in the mold. Avoiding the penetration path of the detection beam by positioning the seam helps maintain the uniformity of the refractive index of the medium within the optical path and reduces stray light interference.

[0040] Example 2 In another typical embodiment of the present invention, such as Figure 1 , Figure 2As shown, a method for manufacturing an optical detection microbial culture container is provided, for manufacturing the optical detection microbial culture container as in Example 1, comprising: First, transparent engineering plastic raw materials are injected into an injection mold with corresponding cavities for injection molding.

[0041] Secondly, at least two sets of recessed structures and / or raised structures of different widths are formed in one step in the detection area 3 of the bottle bottom 2 through the cavity of the injection mold, so that the sidewalls of the recessed structures and / or raised structures are formed into a continuous and closed solid structure, which serves as the detection area 3.

[0042] Finally, after cooling and solidification, the mold is opened and demolded to obtain a completely transparent container or independent parts that can be assembled to form a container.

[0043] The injection mold cavity design has metal cores that are opposite in phase to each other, corresponding to the detection area 3 at the bottom of the bottle. During the holding pressure stage, the high-temperature melt is tightly bonded to the surface of the metal core. As the cooling water channels transfer heat, the detection optical path between the opposite sidewalls is determined by the thickness parameters of the mold core.

[0044] For the stepped detection channels 9, during the mold opening and demolding operation, the core used to form the stepped structure in the injection mold is controlled to be pulled out in a straight line in a single direction. Since the detection channels 9 are arranged sequentially according to size and are collinear, the progressive steps make it less likely for the core to be obstructed or subjected to backlash along the straight path of extraction. The unidirectional core-pulling mechanism reduces the assembly of multi-directional sliders, thereby reducing the probability of leaving parting lines on the transparent optical sidewalls and improving the optical flatness of the surface through which the detection beam penetrates. The resulting independent component is then sealed and fixed using the aforementioned welding or gluing processes to obtain a finished container with good optical path consistency.

[0045] Example 3 In a typical embodiment of the present invention, such as Figure 3 , Figure 4 As shown, an optical detection microbial culture container is presented.

[0046] For cultivation conditions with specific requirements for non-transparency or high intensity, a composite structure combining an opaque main body with a transparent optical window 10 is adopted.

[0047] The optical detection microbial culture container includes a body 1 and a bottom 2. The bottom 2 has a detection area 3 through which a detection beam passes. The detection area 3 has at least two sets of detection channels 9 of different widths. The detection channels 9 are formed by a recessed structure towards the interior of the container and / or a convex structure towards the exterior of the container. The interior of the detection channels 9 forms a fluid space 8 communicating with the interior of the container to contain the culture medium. The distance between the opposite side walls of the detection channels 9 along the direction through which the detection beam passes is the detection optical path.

[0048] Unlike Example 1, in this example, the bottle bottom 2 is made of an opaque material, and the detection channel 9 has through holes on opposite side walls. A transparent lens is sealed and embedded in the through hole, and the transparent lens constitutes an optical window 10 for the detection beam to pass through the culture medium.

[0049] In this structure, the inner surface of the transparent lens on the side through which the detection beam enters remains parallel to the inner surface of the transparent lens on the side through which it exits, and the vertical distance between the two defines the actual detection optical path. At the same time, the gap at the intersection of the transparent lens and the opaque sidewall can also be filled with adhesive to form a chamfered slope, ensuring smooth hydrodynamics of the culture medium in the local space.

[0050] When using opaque materials such as metals or opaque engineering plastics, stray ambient light can be limited from entering the container from the bottom for photosensitive microorganisms. Metals also possess high yield strength and thermal conductivity, improving the heat exchange efficiency of the culture medium and enhancing its resistance to autoclaving. In this case, the specific optical path length is defined by the distance between the inner surfaces of the two corresponding transparent lenses in contact with the culture medium.

[0051] In terms of channel arrangement, at least two sets of detection channels 9 are collinearly distributed, and the direction of collinear distribution extends radially or chordally along the bottom of the bottle 2; when there are three or more sets of detection channels 9, each set of detection channels 9 is arranged sequentially according to the size of its detection optical path, so that the sidewall of the concave or convex structure is in a stepped structure, which reduces the probability of interference when cutting or molding the opaque base.

[0052] In terms of assembly and molding, the bottle body 1 and bottle bottom 2 are independently molded components, with the bottle bottom 2 being machined. During assembly, the edges of the bottle bottom 2 and the edges of the bottle body 1 are sealed and fixed using ultrasonic welding, hot melt welding, adhesive bonding, threaded connection, or snap-fit ​​methods. For example, when the machined metal bottle bottom 2 is fitted to the plastic bottle body 1, adhesive bonding or threaded connection with a sealing ring is preferred, utilizing the elastic interface of the cured adhesive or sealing ring to absorb the thermal strain caused by the difference in thermal expansion coefficients between the dissimilar materials.

[0053] Since this embodiment typically involves the joining of dissimilar materials, such as an opaque metal bottle bottom 2 and a transparent plastic bottle body 1, mechanical threaded connections or adhesive bonding processes are more suitable. For example, the metal bottle bottom 2 has external threads, and the plastic bottle body 1 has internal threads. The two are screwed together to press the internal silicone gasket to achieve a water-blocking seal. This method is not limited by the difference in melting points of the materials. If an adhesive bonding process is used, a high-performance structural adhesive is applied to the annular nested edges of the two materials. The elastic network of the cured structural adhesive buffers the interface to absorb the thermal strain caused by the difference in the coefficients of thermal expansion of the dissimilar materials, preventing the joint interface from cracking and delaminating during drastic temperature rises and falls.

[0054] Example 4 In another typical embodiment of the present invention, such as Figure 3 , Figure 4 As shown, a method for manufacturing an optical detection microbial culture container is provided, for manufacturing the optical detection microbial culture container as in Example 1, comprising: The first step involves machining the bottle bottom 2 from an opaque material, and then machining at least two sets of recessed and / or raised structures of different widths on the bottle bottom 2. If a metal alloy is used for the opaque body, excess material is removed by milling and turning using a CNC machining center. Because the machining feed is controlled by a closed-loop servo system, the dimensional tolerances of different step reference surfaces can be well controlled.

[0055] The second step is to open through holes on the opposite side walls of the recessed structure and / or raised structure, with the through holes penetrating the side walls along the direction of the detection beam.

[0056] The third step is to embed and seal the transparent lens in the through hole to form an optical window 10.

[0057] In the step of creating a through hole, if the opaque body is made of opaque engineering plastic, the through hole is directly formed by the pre-reserved column structure on the mold used in the molding process. If the opaque body is made of metal, the through hole is created by drilling after the bottle bottom 2 is formed. The former is suitable for injection molding of opaque plastics, reducing stress concentration caused by secondary processing; the latter is suitable for machining of metal substrates.

[0058] Accordingly, in the step of embedding and sealing the transparent lens in the through hole, the transparent lens is placed in the mold and integrally injection molded with the opaque material using an insert injection molding process, or an adhesive is used to bond and fix the molded transparent lens in the through hole.

[0059] When both the substrate and the lens are compatible polymers, insert injection molding can induce interpenetration of microscopic molecular chains, forming a high-strength fluid seal. When the substrate is metal and the lens is inorganic glass, medical-grade silicone or UV adhesive is used as the bonding agent. This not only provides a connection, but its elastic network characteristics can also absorb, to some extent, the shear stress at the metal-glass interface caused by autoclaving, reducing the risk of lens interface delamination. Through dispersion processing and composite assembly, high optical path length accuracy is achieved while also giving the container bottom a wider range of environmental adaptability.

[0060] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. An optical detection microbial culture container, characterized in that, It includes the bottle body and the bottle bottom, with a detection area on the bottle bottom for the detection beam to pass through; The detection area is provided with at least two sets of detection channels of different widths, and the detection channels are formed by a recessed structure toward the inside of the container and / or a convex structure toward the outside of the container; The detection channel forms a fluid space inside the container to hold the culture medium. The distance between the two opposite side walls of the detection channel along the direction through which the detection beam passes is the detection optical path. The detection area is integrally molded from transparent engineering plastic, and the detection beam penetrates the side wall of the detection channel and the culture medium filling it.

2. The optical detection microbial culture container as described in claim 1, characterized in that, The at least two sets of detection channels are collinearly distributed, and the direction of the collinear distribution extends radially or chordally along the bottom of the bottle; when there are three or more sets of detection channels, each set of detection channels is arranged in sequence according to the size of its detection optical path, so that the sidewall of the concave structure or convex structure has a stepped structure.

3. The optical detection microbial culture container as described in claim 1 or 2, characterized in that, The bottle body and bottle bottom are separately formed components, and the edge of the bottle bottom is sealed and fixed to the edge of the bottle body by ultrasonic welding, hot melt welding, adhesive bonding, threaded connection or snap-fit.

4. The optical detection microbial culture container as described in claim 1 or 2, characterized in that, The container includes a left half shell and a right half shell that are formed independently. The left half shell and the right half shell are spliced ​​and fixed together, and the splice seam between the left half shell and the right half shell is located outside the detection area.

5. A method for manufacturing an optical detection microbial culture container, characterized in that, For manufacturing an optical detection microbial culture container as described in any one of claims 1-4, comprising: Transparent engineering plastic raw materials are injected into an injection mold with corresponding cavities for injection molding. At least two sets of recessed structures and / or raised structures of different widths are formed in one step in the detection area of ​​the bottle bottom through the cavity of the injection mold, so that the sidewalls of the recessed structures and / or raised structures are formed into a continuous and closed solid structure, which serves as the detection area. After cooling and solidification, the mold is opened and demolded to obtain a completely transparent container or independent parts that can be assembled to form a container.

6. An optical detection microbial culture container, characterized in that, It includes the bottle body and the bottle bottom, with a detection area on the bottle bottom for the detection beam to pass through; The detection area is provided with at least two sets of detection channels of different widths, and the detection channels are formed by a recessed structure toward the inside of the container and / or a convex structure toward the outside of the container; The detection channel forms a fluid space inside the container to hold the culture medium. The distance between the two opposite side walls of the detection channel along the direction through which the detection beam passes is the detection optical path. The bottom of the bottle is made of opaque material, and the detection channel has through holes on the side walls. A transparent lens is sealed and embedded in the through hole, which forms an optical window for the detection beam to pass through the culture medium.

7. The optical detection microbial culture container as described in claim 6, characterized in that, The at least two sets of detection channels are collinearly distributed, and the direction of the collinear distribution extends radially or chordally along the bottom of the bottle; when there are three or more sets of detection channels, each set of detection channels is arranged in sequence according to the size of its detection optical path, so that the sidewall of the concave structure or convex structure has a stepped structure.

8. The optical detection microbial culture container as described in claim 6, characterized in that, The bottle body and bottle bottom are separately formed components. The bottle bottom is formed by machining, and the edge of the bottle bottom is sealed and fixed to the edge of the bottle body by ultrasonic welding, hot melt welding, adhesive bonding, threaded connection or snap-fit.

9. A method for manufacturing an optical detection microbial culture container, characterized in that, For manufacturing an optical detection microbial culture container as described in any one of claims 6-8, comprising: The bottle bottom is formed using an opaque material, and at least two sets of recessed and / or raised structures of different widths are formed on the bottle bottom. Through holes are made on the opposite side walls of the recessed structure and / or the raised structure, and the through holes penetrate the side walls along the direction of the detection beam; A transparent lens is embedded and sealed within the through-hole to form an optical window.

10. The method for manufacturing an optical detection microbial culture container as described in claim 9, characterized in that, In the step of creating a through hole, the through hole is created by drilling after the bottle bottom has been formed. In the step of embedding and sealing the transparent lens in the through hole, the transparent lens is placed in the mold and integrally injection molded with the opaque material using an insert injection molding process, or an adhesive is used to bond and fix the molded transparent lens in the through hole.

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