Diaporthe phaseolorum zhong 9 guo 8 strain and application thereof

Abstract

The application provides a strain of Diaporthe phaseolorum The strain and application thereof are related to the technical field of microorganisms. Diaporthe phaseolorum The strain has been preserved in the China General Microbiological Culture Collection Center on March 23, 2026, and the preservation number is CGMCC No.42656. The strain is an endophytic fungus of Illicium verum, has excellent inhibiting effects on pathogenic fungi such as Alternaria alternata, Colletotrichum gloeosporioides and Diaporthe spp., has broad-spectrum bacteriostatic activity, and has a remarkable biological control effect.

Description

Technical Field

[0001] This invention relates to the field of microbial technology, specifically to a strain Diaporthe phaseolorum 9 fruit strains and their applications. Background Technology

[0002] During the growth of star anise, it is accompanied by a variety of diseases and pests, including blight, anthracnose, gray mold, star anise inchworm, and star anise leaf beetle. These diseases and pests have certain harms and effects on the growth of star anise, leading to the death of star anise trees. After star anise trees are affected by diseases and pests, their vigor is weakened, yield is reduced, they do not bear fruit, and they may even die.

[0003] Traditional agricultural control measures have limitations; they are not very effective against specific diseases and cannot be used as emergency measures to deal with large-scale disease outbreaks. Chemical control may lead to soil and water pollution, disrupt the ecological balance, increase pest resistance, and increase economic costs. Improper use of traditional chemical control methods can cause phytotoxicity to plants, lead to pesticide resistance in pathogens, and may also kill beneficial microorganisms in the plant microenvironment.

[0004] When star anise is used as a daily seasoning, pesticide residues must be considered to ensure food safety. In recent years, with increasing emphasis on food safety and environmental protection, environmentally friendly and green production-compliant biological control has become an important means of ensuring safe agricultural and forestry production and controlling plant diseases.

[0005] Biological control boasts advantages such as environmental friendliness, low susceptibility to drug resistance, sustained control effectiveness, and significant promotion of biodiversity and economic benefits. Plant endophytic fungi, as a potential biological control resource, can control various plant pathogens and promote plant growth and development. Plant endophytic fungi refer to microbial groups that reside within the host plant at specific growth stages without causing significant disease. These microorganisms are widely distributed in various plant species, exhibiting diverse community characteristics. Their microbial communities mainly include three major categories: fungi, bacteria, and actinomycetes. These microorganisms primarily colonize the interstitial spaces or cells of plant leaves, seeds, flowers, stems, fruits, and roots, co-evolving with the host and forming a mutually beneficial symbiotic relationship during evolution. They also produce a rich variety of secondary metabolites that can inhibit the growth of various pathogens, making them valuable for plant disease control and promoting plant growth. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a [missing information - likely a type of plant]. Diaporthe phaseolorum 9 fruit strains and their applications Diaporthe phaseolorum (Illicium verum) strain 9 of the fruit belongs to the endophytic fungi of Illicium verum. It has excellent inhibitory effects on pathogens such as Alternaria alternata, Anthracnose spp. of Clivia minima, and Illicium verum. It also has broad-spectrum antibacterial activity and significant biological control effect.

[0007] To achieve the above objectives, the present invention provides the following technical solution: One plant Diaporthe phaseolorum The strain 9 of the fruit was deposited on March 23, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42656.

[0008] The above Diaporthe phaseolorum The *Diaporthe phaseolorum* strain 9 and a combination thereof can be used to control *Staphylococcus aureus*. Botryosphaeria dothidea Clivia anthracnose fungus ( Colletotrichum cliviicola ), fruit anthracnose ( Colletotrichum fructicola ), Hari anthrax bacteria ( Colletotrichum horii ), Colloidal anthrax bacteria ( Colletotrichum gloeosporioides Fusarium graminearum ( ), Fusarium graminearum Fusarium oxysporum ( Fusarium oxysporum ), short-pointed inter-seat housing ( Diaporthe apiculata ), amygdala shell ( Diaporthe amygdali ), fennel intercalation shell ( Diaporthe foeniculina ), rubber interlocking housing ( Diaporthe heveicola ), Xishuangbanna interlocking shell ( Diaporthe xishuangbanica ), plush interlocking shell ( Diaporthe velutina ), Clostridium difficile ( Neofusicoccum parvum ), Subkeratoid disc polychaete ( Pestalotia subcuticularis Alternaria alternifolia ( ) Alternaria alternata ), Pineapple black spore ( Periconia ananasi At least one of the following can be a plant disease caused by a pathogen:

[0009] Preferably, the plant diseases include blight, anthracnose, and gray mold.

[0010] Preferably, the plant disease is a disease of star anise tree.

[0011] This invention provides a plant Diaporthe phaseolorum The advantages of the 9-fruit 8 strain compared to existing technologies are: This invention separates from star anise Diaporthe phaseolorum The endophytic fungus *Zhong 9 Guo 8* was investigated, and it was verified that this strain can effectively antagonize a variety of plant pathogenic fungi, especially those related to anise blight, anthracnose, and gray mold. This provides a new approach to the prevention and control of anise diseases and reduces the pollution of plants and soil by chemical reagents. Attached Figure Description

[0012] Figure 1 This is a colony morphology diagram of strain Z9G8; Figure 2Phylogenetic tree of strain Z9G8; Figure 3 This is a schematic diagram of the plate confrontation experiment results of Z9G8 against Fusarium graminearum in the embodiments of this application. From left to right, the diagram shows the front view of the confrontation plate, the back view of the confrontation plate, and the control plate with only Fusarium graminearum growing on it. Figure 4 This is a schematic diagram of the plate confrontation experiment results of Z9G8 on short-fiber intercropped shells in the embodiments of this application. From left to right, it shows the front view of the confrontation plate, the back view of the confrontation plate, and the control image of the plate with only short-fiber intercropped shells growing. Figure 5 This is a schematic diagram of the plate confrontation experiment results of Z9G8 against Alternaria alternifolia in the embodiments of this application. From left to right, the diagram shows the front view of the confrontation plate, the back view of the confrontation plate, and the control plate with only Alternaria alternifolia growing on it. Figure 6 This is a schematic diagram of the plate confrontation experiment results of Z9G8 against the surrounding small cluster shell in the embodiments of this application. From left to right, it shows the front view of the confrontation plate, the back view of the confrontation plate, and the control image of the plate with only the surrounding small cluster shell growing. Figure 7 This is a schematic diagram of the plate confrontation experiment results of Z9G8 on Xishuangbanna intercropping shell in the embodiments of this application. From left to right, it is a front view of the confrontation plate, a back view of the confrontation plate, and a control image of the plate with only Xishuangbanna intercropping shell growing. Figure 8 This is a schematic diagram of the colony growth of isolated star anise pathogenic fungi. Detailed Implementation

[0013] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0014] In the following embodiments Diaporthe phaseolorum The strain 9 of *Zhongguo* was deposited on March 23, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42656. The depository address is: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. It is recommended that the strain be classified and named as follows: Diaporthe phaseolorum It is the intercalary shell of the common bean.

[0015] Example 1: Diaporthe phaseolorumIsolation and identification of Z9G8 strain: I. Experimental Materials and Methods 1. Experimental materials: Different tissue parts of healthy star anise were collected from star anise planting areas in Funing County, Wenshan Prefecture, Yunnan Province. The samples were labeled, stored in sealed bags, and brought back to the laboratory at low temperature.

[0016] 2. Isolation and purification of endophytic fungi (1) Test instruments: Petri dishes, Erlenmeyer flasks, test tubes, laminar flow hoods, microscopes, autoclaves, and incubators.

[0017] Test culture medium formulation: 200 g potatoes (peeled), 20 g sucrose (or glucose), 0.5 g magnesium sulfate, 1 g potassium dihydrogen phosphate, 1000 mL water, 20 g agar.

[0018] (2) Test reagent: Sterile water, anhydrous ethanol, 0.1% mercuric chloride solution, 75% ethanol.

[0019] 3. Tissue isolation of endophytic bacteria: Select healthy, disease-free star anise flowers, fruits, and leaves. First, rinse them with a 75% ethanol solution to remove surface impurities. After drying, place them in a clean bench for UV sterilization. Use a sterile scalpel to cut the plant tissue into small square pieces. Immerse each piece in a 0.1% mercuric chloride solution for 10-15 seconds, then remove and rinse again in a 75% ethanol solution to remove residual mercuric chloride. Place the plant tissue pieces on sterile filter paper to absorb surface liquid. Place the sterilized plant tissue pieces on a sterilized enriched culture medium for incubation. Completely seal the inoculated culture dish with sealing film and incubate at a constant temperature of 25°C to observe for mycelial growth.

[0020] 4. Purification of endophytic bacteria: The isolated endophytic fungus strain Z9G8 was purified. The purified strain was inoculated into the center of PDA plates and incubated in the dark at 25°C, with seven replicates per strain. Colony diameter (cross-hatching method), color, texture, pigment production, and presence of conidiophores were observed daily for nine consecutive days. Photographs of the front and back of the colonies were taken after six days of incubation. Figure 1 The growth status record is shown in Table 1.

[0021] Table 1

[0022] (1) Reagents and materials Alcohol lamp, centrifuge, centrifuge tubes, pipette, pipette tip, syringe, conical flask, 1200 mL wide-mouth bottle, microwave oven, distilled water, ITS5 primers, mask, rubber gloves, agarose, BM2000+ DNA Maker reagent, fluorescent dye, bromophenol blue dye solution, TAE electrophoresis buffer, mix, sterile water, anhydrous ethanol, disinfectant alcohol, electronic scale, PCR instrument, D3195-01HP Fungal DNA Mini Kit (50) reagent kit.

[0023] (2) DNA extraction Reagent preparation: Chloroform-isoamyl alcohol mixture (24:1): Mix 96 mL of chloroform with 4 mL of isoamyl alcohol. After preheating the CTAB solution at 65°C, add 1 mL of reducing agent to the CTAB solution (at a volume ratio of 500:1) and mix.

[0024] Operating steps: a. Perform the operation in a clean bench. Use a syringe needle to pick up about 0.5 g of mycelium, transfer it to a 1.5 mL centrifuge tube, and add 700 μL of 2×CTAB extraction solution (with reducing agent added).

[0025] b. Place the centrifuge tube in a 65°C water bath for 30 minutes, gently shaking the centrifuge tube every 10 minutes or so.

[0026] c. After the water bath is complete, cool for 2 minutes, add 0.5 mL of chloroform-isoamyl alcohol mixture, and shake vigorously to mix. Centrifuge at 10000 rpm for 10 minutes.

[0027] d. Transfer the supernatant from the centrifuge tube in the previous step into a new centrifuge tube.

[0028] Note: After centrifugation, the mixture should separate into three layers: an upper aqueous phase, a middle layer of protein and plant debris, and a lower organic phase. Avoid touching the protein and organic phases when aspirating. If you accidentally aspirate the lower or middle layer, or if the middle layer is too thick, it is recommended to aspirate only a small amount of the aqueous phase, or add 500 μL of chloroform-isoamyl alcohol mixture after aspirating, mix well, centrifuge again, and collect the supernatant.

[0029] e. Add an equal volume of isopropanol to the supernatant and mix by inverting. Incubate at -20°C for 40 min to precipitate, then centrifuge at 12000 rpm for 10 min and discard the supernatant.

[0030] f. Wash the precipitate once with 75% ethanol, centrifuge at 12000 r / min for 5 min, discard the supernatant, let the precipitate dry naturally, dissolve it in 50 μL of deionized water or TE, add 1 μL of RNase (20 μg / mL), and incubate at 37℃ for 30 min.

[0031] g. Store at -20℃ for later use.

[0032] (3) PCR amplification and electrophoresis detection The PCR amplification reaction system used was a 25 μL reaction system, which included 12.5 μL of 10x amplification buffer containing mix reagent, 0.5 μL each of the forward primer (ITS1) and reverse primer (ITS4), 1.0 μL of DNA sample, and 10.5 μL of ddH2O to a final volume of 25 μL.

[0033] Take 3.0 μL of the extracted and amplified DNA and mix it with 0.3 μL of bromophenol blue solution (40% sucrose, 0.25% bromophenol blue). Electrophoresis is performed on a 1% agarose gel containing 5‰ Super GelRed nucleic acid dye in 1.0×TAE buffer at an electrophoresis voltage of 90 V for 30 min. After electrophoresis, the gel is removed and the molecular weight of the obtained PCR is observed in a gel imaging system. The results are recorded and saved.

[0034] Sequencing: The ITS amplified fragment samples obtained by PCR were sent to Beijing Qingke Biotechnology Co., Ltd. for sequencing, and finally the forward ITS sequence fragment file of the sample was obtained.

[0035] Data Analysis: After obtaining the sequencing files, the peaks were first observed using BioEdit software, and sequences without impurities were copied. Sequence alignment was performed using the NCBI website, and the alignment gene library data used in NCBI was downloaded and saved. The obtained strain sequence was then compared with the downloaded sequences of similar species.

[0036] The results showed that after BLAST alignment, Z9G8 had 100% similarity to *Diaporthephaseolorum* in the GenBank database. To further clarify its taxonomic position, reference sequences of related genera were downloaded from GenBank, and a phylogenetic tree was constructed using the maximum likelihood (ML) method on the CIPRES website (e.g., ...). Figure 2 (As shown).

[0037] 5. Isolation, culture, and identification of fungal pathogens causing diseases in star anise. 5.1 Isolation and purification of pathogens causing diseases of star anise fungi (1) Experimental materials and tools Specimens of flowers, fruits, branches, and leaves of star anise infected with the disease; potatoes (peeled); double-layered gauze; agar; glucose; magnesium sulfate; potassium dihydrogen phosphate; conical flask; glass rod; inoculation knife; forceps; sealing film; newspaper; rubber bands; petri dishes; 75% alcohol; 0.1% mercuric chloride solution; sterile water; CTBA buffer; chloroform-isoamyl alcohol (PCl=24:1); TBE electrophoresis buffer; 70% ethanol; ITS1 primer; ITS4 primer; anhydrous ethanol; induction cooker; constant temperature incubator; autoclave; clean bench.

[0038] (2) Preparation of culture medium Boil 200 g of peeled potatoes until cooked, filter the solution, and add 20 g of agar, 20 g of glucose, 0.5 g of magnesium sulfate, and 1 g of potassium dihydrogen phosphate to the solution. While still hot, bring the volume to 1 L. Heat the diluted mixture until the agar melts, then dispense it into Erlenmeyer flasks. Seal the flasks with sealing film and await sterilization. Wrap all experimental tools in newspaper, reinforce with rubber bands, and sterilize them along with the Erlenmeyer flasks containing the culture medium at 121°C for 30 min. After sterilization, place the alcohol lamp and other experimental equipment on a clean bench and irradiate with ultraviolet light for 15–20 min for surface disinfection. Once the sterilized culture medium has cooled to a suitable temperature, pour the culture into the dishes. Note: The volume of the culture medium should not exceed two-thirds of the dish's capacity. After pouring, place the dishes on a clean bench to cool and solidify before use.

[0039] (3) Isolation and culture of pathogens causing diseases of star anise The collected anise disease specimens were rinsed with tap water according to the different types of diseases, and then air-dried on a clean bench. Using a sterile inoculation knife, the diseased and healthy tissue junctions were cut into small pieces of 0.5 cm × 0.5 cm. The pieces were then soaked in 75% ethanol for 30 seconds for disinfection. Next, they were transferred to 0.1% mercuric chloride solution for disinfection for 10 seconds each time, and then rinsed with sterile water 2-3 times. Finally, the small pieces of diseased tissue were placed on sterile filter paper to absorb the surface moisture, and then transferred to culture medium. Three tissue pieces were placed on each culture dish in an equilateral triangle pattern, and the dishes were incubated upside down in a 25°C incubator. The dishes were observed every 48 hours. If colonies were found to grow, the specimens were immediately transferred to a new culture medium.

[0040] (4) Purification and culture of pathogens causing diseases of star anise The mycelial tip purification method was used to purify the pathogenic bacterial colonies that grew out. Diseased tissue was placed on PDA solid medium and cultured until colonies grew. The mycelial tips were cut into small pieces with a sterile inoculation knife, placed on PDA solid medium, and incubated upside down in a 25°C constant temperature incubator to achieve the purpose of strain purification.

[0041] 5.2 Identification of pathogens causing star anise fungal diseases 5.2.1 Morphological identification of pathogens causing diseases of star anise Observation of the plate morphology of the strain: The mycelium was inoculated on the culture medium and incubated at 25°C in a constant temperature incubator. The colony diameter was measured every 3 days. The growth characteristics such as colony morphology, color and texture were observed and recorded until the plate was full.

[0042] 5.2.2 Molecular biological identification of pathogens causing diseases caused by star anise fungi DNA extraction DNA was extracted from mycelial hyphae using the CTAB method. 0.5 g of mycelia were placed in a centrifuge tube, 200 μL of CTAB buffer was added, and the mixture was ground. Then, 600 μL of CTAB buffer was added and mixed thoroughly. The mixture was incubated at 65°C for 1 h, with shaking every 10 min. After cooling, an equal volume of PCI (chloroform:isoamyl alcohol = 24:1) was added, and the mixture was centrifuged at 12000 r / min for 10 min. The supernatant was transferred to a new centrifuge tube, and two volumes of anhydrous ethanol were added. The mixture was incubated at room temperature for 10 min to precipitate the DNA. The tube was centrifuged at 12000 r / min for 20 min, the supernatant was discarded, and the DNA was washed twice with 70% ethanol. The precipitate was collected, the ethanol was air-dried, and the DNA was dissolved in 20 μL of sterile distilled water and stored at -20°C for later use.

[0043] PCR amplification and electrophoresis detection The PCR amplification reaction system used was a 25 μL reaction volume (as shown in Table 2 below), which included 12.5 μL of 10x amplification buffer containing mix reagent, 0.5 μL each of the forward and reverse primers, 1.0 μL of DNA sample, and 10.5 μL of ddH2O to a final volume of 25 μL. 3.0 μL of the extracted and amplified DNA was mixed with 0.3 μL of bromophenol blue solution (40% sucrose, 0.25% bromophenol blue), and electrophoresed on a 1% agarose gel containing 5‰ Super GelRed nucleic acid dye in 1.0×TAE buffer at 90 V for 30 min. After electrophoresis, the gel was removed, and the molecular weight of the PCR was observed using a gel imaging system. The results were recorded and saved.

[0044] Table 2

[0045] The ITS amplified fragment samples obtained by PCR were sent to Beijing Qingke Biotechnology Co., Ltd. for sequencing, and finally the forward ITS sequence fragment file of the sample was obtained.

[0046] Data Analysis After obtaining the sequencing files, the peak values ​​were first observed using BioEdit software. Sequences without contaminating peaks were then copied. Sequence alignment was performed using the NCBI website. The alignment database values ​​used in NCBI were downloaded and saved. The obtained strain sequence was then compared with downloaded sequences of similar species, and its taxonomic position was determined using a phylogenetic tree. The identified pathogen was *Staphylococcus aureus* (…). Botryosphaeria dothidea Clivia anthracnose fungus ( Colletotrichum cliviicola ), fruit anthracnose ( Colletotrichum fructicola ), Hari anthrax bacteria ( Colletotrichum horii ), Colloidal anthrax bacteria ( Colletotrichum gloeosporioides Fusarium graminearum ( ), Fusarium graminearum Fusarium oxysporum ( Fusarium oxysporum ), short-pointed inter-seat housing ( Diaporthe apiculata ), amygdala shell ( Diaporthe amygdali ), fennel intercalation shell ( Diaporthe foeniculina ), rubber interlocking housing ( Diaporthe heveicola ), Xishuangbanna interlocking shell ( Diaporthe xishuangbanica ), plush interlocking shell ( Diaporthe velutina ), Clostridium difficile ( Neofusicoccum parvum ), Subkeratoid disc polychaete ( Pestalotia subcuticularis Alternaria alternifolia ( ) Alternaria alternata ), Pineapple black spore ( Periconia ananasi ), the specific colony growth of pathogens as follows Figure 8 As shown.

[0047] 6. Antibacterial experiments of Z9G8 against various pathogens: Both the pathogens and the Z9G8 strain were taken from laboratory-preserved test tube slants. Before the experiment, in a clean bench, 5 mm diameter mycelial discs were uniformly taken from the edge of the activated colonies using a sterile punch and transferred to the center of fresh PDA plates. These were then incubated at 25 ℃ for simultaneous activation. The activated strains were used as inoculum for subsequent confrontation cultures to ensure that the growth vigor of all strains was essentially consistent.

[0048] The antibacterial activity of Z9G8 against pathogens was determined using the plate confrontation culture method. Activated pathogens and Z9G8 colony edge discs were collected using a sterile punch (5 mm inner diameter). In a clean bench, the pathogen discs were inoculated in the center of the plate with the mycelial side down. Z9G8 endophytic fungal discs were inoculated at four equidistant points, also with the mycelial side down, forming an inoculation pattern with the pathogen in the center and endophytic fungi around the edges. The entire process adhered to the principle of activating from the same batch and inoculating at the same time. A control group was used, consisting only of pathogen discs activated from the same batch inoculated in the center of the plate without endophytic fungi inoculation. The control group and treatment group used the same culture medium, disc source, activation batch, inoculation time, and incubation temperature and time to eliminate systematic errors. Each group had at least five replicates. After inoculation, the plates from both the treatment and control groups were sealed and inverted in a 25°C incubator for 5 days. Colony growth and changes at the confrontation interface were observed daily. On day 5, when the pathogens in the control group were about to completely cover the plates, all plates were removed for measurement and recording. The antibacterial effect was determined using the cross-sectional method to measure colony diameter and inhibition zone diameter.

[0049]

[0050] The specific results are shown in Table 3 below: Table 3

[0051] The above experiments demonstrate that Z9G8 exhibits significant antagonistic effects against a variety of plant pathogens. Confrontation experiments confirmed that this endophytic bacterium can effectively inhibit pathogen mycelial growth and spore germination, providing core materials and technical support for the development of environmentally friendly novel biological pesticides or biocontrol agents.

[0052] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A plant Diaporthe phaseolorum The 9 fruit 8 strains are characterized by, The strain was deposited on March 23, 2026, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 42656.

2. A plant as described in claim 1 Diaporthe phaseolorum 9 fruit 8 strains in the prevention and control of Staphylococcus aureus ( Botryosphaeria dothidea Clivia anthracnose fungus ( Colletotrichum cliviicola ), fruit anthracnose ( Colletotrichum fructicola ), Hari anthrax bacteria ( Colletotrichum horii ), Colloidal anthrax bacteria ( Colletotrichum gloeosporioides Fusarium graminearum ( ), Fusarium graminearum Fusarium oxysporum ( Fusarium oxysporum ), short-pointed inter-seat housing ( Diaporthe apiculata ), amygdala shell ( Diaporthe amygdali ), fennel intercalation shell ( Diaporthe foeniculina ), rubber interlocking housing ( Diaporthe heveicola ), Xishuangbanna interlocking shell ( Diaporthe xishuangbanica ), plush interlocking shell ( Diaporthe velutina ), Clostridium difficile ( Neofusicoccum parvum ), Subkeratoid disc polychaete ( Pestalotia subcuticularis Alternaria alternifolia ( ) Alternaria alternata ), Pineapple black spore ( Periconia ananasi At least one of the following can be used in plant diseases caused by pathogens:

3. The application according to claim 2, characterized in that: The plant diseases mentioned include blight, anthracnose, and gray mold.

4. The application according to claim 2, characterized in that: The plant disease mentioned is a disease of the star anise tree.

5. A composition for preventing and controlling plant diseases, characterized in that: The composition contains accession number CGMCC No. 42656. Diaporthe phaseolorum 9 fruits and 8 strains.

6. The application of the composition for controlling plant diseases as described in claim 5, characterized in that: The composition is used to prevent and treat Staphylococcus aureus ( Botryosphaeria dothidea Clivia anthracnose fungus ( Colletotrichum cliviicola ), fruit anthracnose ( Colletotrichum fructicola ), Hari anthrax bacteria ( Colletotrichum horii ), Colloidal anthrax bacteria ( Colletotrichum gloeosporioides Fusarium graminearum ( ), Fusarium graminearum Fusarium oxysporum ( Fusarium oxysporum ), short-pointed inter-seat housing ( Diaporthe apiculata ), amygdala shell ( Diaporthe amygdali ), fennel intercalation shell ( Diaporthe foeniculina ), rubber interlocking housing ( Diaporthe heveicola ), Xishuangbanna interlocking shell ( Diaporthe xishuangbanica ), plush interlocking shell ( Diaporthe velutina ), Clostridium difficile ( Neofusicoccum parvum ), Subkeratoid disc polychaete ( Pestalotia subcuticularis Alternaria alternifolia ( ) Alternaria alternata ), Pineapple black spore ( Periconia ananasi At least one of the following can be a plant disease caused by a pathogen:

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