Kit for cyanuric acid detection in swimming pools, lyophilized reagents, detection system and method

Abstract

The application discloses a kit, a freeze-dried reagent, a detection system and a method for detecting cyanuric acid in a swimming pool. The kit comprises a box body with a closed cavity, a light transmission window and a normally closed water sample inlet. A melamine reagent freeze-dried composition layer is arranged in the cavity. During detection, the water sample of the swimming pool is quantitatively introduced into the cavity by piercing the water sample inlet, and a complex reaction occurs with the melamine reagent composition therein, generating melamine cyanurate precipitate with a median particle size in the range of 1-10 mu m and forming a uniform suspension. Scattering detection is carried out by using an optical detection module, and rapid and accurate calculation of the cyanuric acid concentration is realized through a calibration curve. The application can realize automatic water taking, reaction, optical detection and cyanuric acid concentration detection output in a whole process, and meets the high-frequency and instant quantitative detection requirements of water quality detection on site.

Description

Technical Field

[0001] This invention relates to the field of swimming pool water quality testing robot technology, and in particular to cyanuric acid detection technology, specifically to a reagent kit, lyophilized reagent, detection system and method for cyanuric acid detection in swimming pools. Background Technology

[0002] The safety of swimming pool water quality directly affects swimmers' health and experience. Disinfection is the core of pool water quality management. Currently, chlorination is the primary disinfection method, with chlorine-containing disinfectants such as sodium hypochlorite, sodium dichloroisocyanurate (DCCNa), and trichloroisocyanuric acid (TCCA) widely used. Cyanuric acid (CYA, chemical formula C3H3O3) plays a crucial role in pool water, primarily originating from the decomposition and residues of chlorine-containing disinfectants. Cyanuric acid accumulates in pool water during the decomposition of chlorine-containing disinfectants, leading to a continuous increase in concentration. Excessively high concentrations not only affect disinfection effectiveness but also pose potential health hazards. When the cyanuric acid concentration exceeds 100 mg / L, the detection rate of coliform bacteria in pool water samples increases significantly, raising the risk of pathogen transmission.

[0003] Currently, the main methods for detecting cyanuric acid in swimming pools include colorimetric / turbidimetric methods (rapid on-site testing) and laboratory instrumental analysis methods (precise quantification). Colorimetric methods involve sampling pool water on-site, conducting a color reaction, and comparing the color change of the test strip with a standard colorimetric card to obtain a semi-quantitative result of the cyanuric acid concentration. The advantages of rapid on-site colorimetric testing are low operational threshold, low cost, and extremely fast detection speed. However, color comparison is greatly affected by ambient lighting conditions and individual visual differences, resulting in high subjectivity, limited accuracy, difficulty in distinguishing similar color levels, and poor repeatability. Therefore, the test strip method is more suitable for frequent, rapid screening and trend monitoring, and not as a precise quantitative analysis method.

[0004] Turbidimetric methods involve sampling pool water on-site with a detector, reacting it with a specific precipitating reagent (such as melamine MEL) to form an insoluble white particulate precipitate (melamine-cyanurate, MCA). The concentration of cyanuric acid is then quantitatively analyzed using turbidity or scattering signals. For example, spectrophotometric turbidimetry automatically matches the detection wavelength within the 340 nm to 800 nm range and calculates the concentration based on Lambert-Beer's law. Currently, the advantage of turbidimetric methods lies in their simplicity, but they require portable equipment and specialized reagent kits to meet the demands of high-frequency inspections. Furthermore, traditional melamine has extremely low solubility in water, resulting in a slow reaction start-up and long dissolution time for powdered reagents, affecting detection efficiency. Additionally, as the precipitate particles continue to grow, the turbidity signal drifts over time, impacting detection accuracy and making it difficult to apply to automated or miniaturized equipment.

[0005] Laboratory instrumental analysis (precise quantification) requires sampling pool water and transferring it to a laboratory for testing using sophisticated instruments, such as spectrophotometry, high-performance liquid chromatography (HPLC), and liquid chromatography-mass spectrometry (LC-MS). This method relies on specialized laboratory equipment and standardized operating procedures, has a long testing cycle, and requires samples to undergo multiple steps such as sampling, transportation, and pretreatment, making it difficult to meet the immediate testing needs of on-site pool water quality monitoring. Summary of the Invention

[0006] In view of the need for rapid and frequent daily monitoring of swimming pool water quality in the prior art and the shortcomings of existing melamine-based detection systems, according to a first aspect of the present invention, a kit for detecting cyanuric acid in swimming pools is provided, comprising:

[0007] The box body has a closed cavity inside, and a layer of test reagents is provided inside the cavity;

[0008] A light-transmitting window located on at least one surface of the housing;

[0009] A water sample inlet is provided on at least one surface of the housing and allows water samples to enter the cavity from the outside of the housing;

[0010] The water sample inlet is set to be normally closed, and the cavity is isolated from the outside of the box when not sampling.

[0011] During the detection process, the water sample enters the cavity and comes into contact with the detection reagent layer to form a suspension. The light-transmitting window allows incident light emitted by the external optical detection module to pass through and is also transmitted through the scattered light that returns after being scattered by the suspension.

[0012] As an optional implementation, the number of light-transmitting windows is two, located on two opposite surfaces of the box body, or on two surfaces distributed at 90°.

[0013] As an optional implementation, the water sample inlet employs a diaphragm layer that can be punctured from the outside.

[0014] As an optional implementation, the diaphragm layer is a thermoplastic polymer film layer.

[0015] As an optional implementation, the diaphragm layer is a one-time sealed thin film layer that can be irreversibly ruptured under external mechanical puncture to form the inlet of the water sample collection channel.

[0016] As an optional implementation, the water sample inlet employs a diaphragm layer with self-sealing channels.

[0017] As an optional implementation, the diaphragm layer is made of an elastomer sheet, which keeps the self-sealing channel closed and elastically deforms in response to an externally applied thrust to open the channel and form the inlet of the water sample collection channel.

[0018] As an optional implementation, the box body is also provided with at least one overflow port, which communicates with the cavity.

[0019] As an optional implementation, the test reagent layer is a melamine freeze-dried layer, which is attached to at least one inner wall surface of the cavity.

[0020] As an optional implementation, the melamine freeze-dried layer is a porous membrane layer formed by in-situ freeze-drying of the melamine reagent composition in the cavity.

[0021] As an optional embodiment, the melamine reagent composition comprises the following components in parts by weight:

[0022] 10-60 parts of melamine powder;

[0023] 25-80 parts of hydrophilic framework material;

[0024] 0.001 to 1 part of Melamine-Cyanurate seed crystals;

[0025] Dispersant 0.1-10 parts;

[0026] 0-5 parts of auxiliary agents;

[0027] The auxiliary agents include at least one of a masking agent, a pH adjuster, and a stabilizer; the melamine reagent composition has a porous membrane structure with a water content of <1%.

[0028] As an optional implementation, the hydrophilic skeleton material is at least one of sugar alcohols, polysaccharides, or hydrophilic polymers.

[0029] As an optional implementation, the hydrophilic skeleton material is at least one of mannitol, trehalose, sorbitol, polyvinylpyrrolidone, and polyethylene glycol.

[0030] As an optional implementation, the dispersant is at least one of PVP, SDS, and Tween surfactants, in a weight ratio of 0.5 to 5 parts.

[0031] As an optional implementation, the masking agent and stabilizer are present in an amount of 0 to 0.5 parts by weight; the pH adjuster is present in an amount of 0 to 5 parts by weight.

[0032] As an optional implementation, the pH adjuster is a phosphate buffer salt with pH=7.

[0033] As an optional implementation, the stabilizer is a cellulose-based or natural / synthetic polymer stabilizer.

[0034] As an optional implementation, the masking agent is either an inorganic salt masking agent or an organic masking agent.

[0035] As an optional implementation, the detection reagent layer dissolves and reacts within 5 to 60 seconds after contacting the water sample entering the cavity. The median particle size of the precipitate particles formed by the reaction is 1 to 10 μm, and the turbidity change is less than ±5% within 30 to 60 seconds after contact.

[0036] According to a second aspect of the present invention, a swimming pool water quality testing system is provided, comprising:

[0037] The aforementioned reagent kit;

[0038] A pool water sampling module, wherein the reagent kit can be externally coupled to the pool water sampling module, and at the coupling interface between the two, the pool water sampling module operates the water sample inlet of the reagent kit to form a water sample sampling channel inlet;

[0039] Optical inspection module;

[0040] Detection data processing module;

[0041] Control module;

[0042] The control module is connected to the pool water sample acquisition module, the optical detection module, and the detection data processing module, and:

[0043] The system controls the pool water sampling module to collect water from the pool and deliver it into the cavity of the reagent kit.

[0044] The optical detection module is controlled to emit detection light signals through the light-transmitting window of the reagent kit into the suspension formed by the reaction inside the cavity, and to receive the light signals scattered back by the suspension.

[0045] The detection data processing module is controlled to calculate the concentration of cyanuric acid in the water sample based on the detection light signal, the returned light signal, and the calibration curve.

[0046] As an optional implementation, the control module controls the pool water sample collection module to deliver water samples into the reagent kit according to a preset sampling amount, so as to keep the water sample volume in the reagent kit constant.

[0047] As an optional implementation, the cavity inside the kit serves as a reaction chamber with a volume of 5 mL to 10 mL.

[0048] As an optional implementation, the pool water sample collection module includes a pump controlled by the control module, which has a water intake side capable of collecting water samples from the pool and a water outlet side capable of operating the water sample inlet from outside the reagent kit to form the water sample collection channel inlet.

[0049] As an optional implementation, the optical detection module includes a red LED for emitting detection light signals and a photosensitive PD for receiving the returned light signals.

[0050] As an optional implementation, the red LED is an LED emitter with a wavelength of 850 nm.

[0051] As an optional implementation, the pool water sample collection module, optical detection module, detection data processing module, and control module are integrated into a single detection device;

[0052] The reagent kit can be coupled to the detection device and connected to the pool water sample collection module.

[0053] As an optional implementation, the detection device is a swimming pool robot.

[0054] In a third aspect of the present invention, a method for detecting cyanuric acid in swimming pools using the aforementioned kit is also provided, comprising the following steps:

[0055] Operate the reagent kit and establish the entrance for the water sample collection channel;

[0056] A pool water sample is introduced into the cavity of the reagent kit through the water sample collection channel inlet, so that the water sample comes into contact with the detection reagent layer in the cavity and reacts to form a suspension in the cavity.

[0057] After a certain reaction time, a detection light signal is emitted into the resulting suspension;

[0058] Receive the optical signal returned after being scattered by the suspension;

[0059] The concentration of cyanuric acid in the water sample is calculated based on the detected light signal, the returned light signal, and the calibration curve.

[0060] As an optional implementation, the calibration curve is set to be obtained in advance under conditions of melamine and cyanuric acid in molar excess.

[0061] As an optional implementation, the method utilizes a pool robot to achieve sampling and detection output.

[0062] The kit, lyophilized reagent, and detection system for cyanuric acid detection in swimming pools, as described in the above embodiments, offer significant advantages over existing technologies, including:

[0063] (1) The reagent kit for detecting cyanuric acid in swimming pool water samples adopts a disposable, integrated normally closed box structure design. The box body forms a closed cavity with a built-in detection reagent layer. The water sample inlet adopts a normally closed design, which isolates the cavity from the outside world when not sampling, avoiding the reagent from getting damp or contaminated. It also uses a double light-transmitting window to cooperate with scattering turbidimetric detection, which can achieve pre-packaging, long-term storage / transport stability and rapid and accurate coupling with detection equipment.

[0064] (2) The melamine lyophilized reagent composition is lyophilized in situ in the cavity of the kit to form a porous membrane layer with a water content of <1%. Utilizing the capillary water absorption effect of the porous structure, it can achieve dissolution of water sample in seconds (<2 seconds) after contact, eliminating the disadvantage of slow dissolution of traditional melamine powder. At the same time, by introducing trace amounts of MCA seeds, the traditional heterogeneous nucleation path is changed through the pre-nucleation induction mechanism. With the synergistic inhibition effect of stabilizers and dispersants, the median particle size of the precipitate is locked in the ideal range of 1 μm to 10 μm, forming a stable suspension, which restricts the uncontrolled growth and aggregation of precipitate particles. Through comprehensive kinetic regulation, the consistency and controllability of the reaction start and end point are ensured.

[0065] (3) The kit can be quickly and detachably coupled to testing equipment such as pool robots. Through the integrated water sample collection, optical detection, data processing and control modules, the kit is used to achieve rapid and fully automated water collection, reaction, optical detection and cyanuric acid (CYA) concentration detection output by adopting the 90° side scattering turbidimetric method, which meets the high frequency and real-time quantitative detection needs of pool water quality on site.

[0066] It should be understood that all combinations of the foregoing concepts and the additional concepts described in more detail below may be considered part of the inventive subject matter of this disclosure, provided that such concepts do not contradict each other. Furthermore, all combinations of the claimed subject matter are considered part of the inventive subject matter of this disclosure.

[0067] The foregoing and other aspects, embodiments, and features of the teachings of the present invention will be more fully understood from the following description in conjunction with the accompanying drawings. Other additional aspects of the invention, such as features and / or beneficial effects of exemplary embodiments, will become apparent from the following description or may be learned through practice of specific embodiments according to the teachings of the present invention. Attached Figure Description

[0068] The accompanying drawings are not intended to be drawn to scale. In the drawings, each identical or nearly identical component shown in the various figures may be denoted by the same reference numeral. For clarity, not every component is labeled in each figure. Embodiments of various aspects of the invention will now be described by way of example and with reference to the accompanying drawings.

[0069] Figure 1This is a schematic diagram of the overall structure of a reagent kit for detecting cyanuric acid in swimming pools according to an embodiment of the present invention.

[0070] Figure 2 This is a schematic diagram of a half-section of a reagent kit for detecting cyanuric acid in swimming pools according to an embodiment of the present invention.

[0071] Figure 3 This is a schematic diagram of a swimming pool water quality testing system according to an embodiment of the present invention.

[0072] Figure 4 This is a schematic diagram of the coupling method between the reagent kit and the pool robot in a pool water quality testing system according to an embodiment of the present invention.

[0073] Figure 5 This is a schematic diagram of another coupling method between the reagent kit and the pool robot in the pool water quality testing system according to an embodiment of the present invention.

[0074] Figure 6 This is a schematic flowchart of a method for detecting cyanuric acid in swimming pools according to an embodiment of the present invention.

[0075] Figure 7 This is a schematic diagram of the linear fitting results of the scattering turbidity after the complexation reaction of melamine in different concentrations of cyanuric acid solutions according to an embodiment of the present invention.

[0076] Figure 8 This is a schematic diagram of the linear fitting results of the scattering turbidity after the lyophilized reagent undergoes a complexation reaction in cyanuric acid solutions of different concentrations (different molar ratios) according to an embodiment of the present invention. Detailed Implementation

[0077] To better understand the technical content of the present invention, specific embodiments are described below in conjunction with the accompanying drawings.

[0078] Various aspects of the invention are described in this disclosure with reference to the accompanying drawings, which illustrate numerous illustrative embodiments. The embodiments of this disclosure are not necessarily intended to encompass all aspects of the invention. It should be understood that the various concepts and embodiments described above, as well as those described in more detail below, can be implemented in any of many ways, because the concepts and embodiments disclosed herein are not limited to any particular implementation. Furthermore, some aspects of the invention disclosed may be used alone or in any suitable combination with other aspects of the invention disclosed.

[0079] {Reagent Kit 100}

[0080] Combination Figure 1 , Figure 2As shown, the kit 100 for detecting cyanuric acid in swimming pools according to an example of the present invention includes a housing 101, a detection reagent layer 102, a light-transmitting window 103, and a water sample inlet 104.

[0081] The box body 101 may be made of waterproof materials such as rigid plastic, forming a closed cavity 110 inside the box body. Figure 3 Example), combined Figure 2 As shown, a test reagent layer 102 is provided inside the cavity. The test reagent layer 102 is used to form a suspension when it comes into contact with the water sample to detect the concentration of cyanuric acid in the pool water sample.

[0082] As an optional example, the aforementioned detection reagent layer 102 may in particular employ a melamine detection reagent based on a melamine-cyanuric acid reaction system, which is attached to the inside of the cavity by means of coating, lyophilization, etc. It can generate an insoluble precipitate (melamine-cyanurate) based on the reaction of melamine and cyanuric acid, thereby facilitating quantitative analysis and detection through turbidity or scattering signals.

[0083] The light-transmitting window 103 is located on at least one surface of the housing 101. In this optional example, especially for detection based on quantitative analysis of scattering signals, two light-transmitting windows 103 are provided in the housing, located on the same side surface of the housing, or on two opposite surfaces, or on two surfaces distributed at 90°.

[0084] As an optional example, the light-transmitting window 103 is made of a high-transmittance material, including but not limited to PMMA, PC, etc.

[0085] like Figure 1 , 2 In the example shown, a water sample inlet 104 is provided on at least one surface of the housing 101, allowing a water sample to enter the cavity 110 from the outside of the housing 101 during detection, thereby enabling it to contact and react with the detection reagent layer 102 (e.g., melamine detection reagent) inside the cavity.

[0086] In an embodiment of the present invention, the water sample inlet 104 is set to a normally closed state, and the connection between the cavity 110 and the outside of the box 101 is isolated in the non-sampling state.

[0087] As an optional implementation, the water sample inlet 104 employs a diaphragm layer that can be punctured from the outside, such as a thermoplastic polymer film layer, including but not limited to diaphragm layers made of materials such as PE, PP, PVC, HIPS, PET, and TPU. In particular, diaphragm layers made of materials such as PE, PP, and PVC with controllable low puncture strength can be selected, allowing the water sample collection channel to be opened by external operation, connecting the cavity 110 to the outside of the box 101.

[0088] Preferably, the diaphragm layer is a one-time sealed thin film layer that can be irreversibly ruptured under external mechanical puncture, thereby forming the inlet of the water sample collection channel.

[0089] In another alternative embodiment, the water sample inlet 104 employs a diaphragm layer with a self-sealing channel. For example, the diaphragm layer is an elastomer sheet, which maintains the self-sealing channel closed by its elasticity, isolating the inside from the outside, and responds to an externally applied thrust, such as through a tube, a conical nozzle, or a conical interface, causing the elastomer sheet to elastically deform, thereby opening the channel and forming a water sample collection channel inlet, allowing the water sample to enter the reagent kit 100.

[0090] In particular, the box body 101 can be made of an opaque material to reduce and avoid environmental interference during testing.

[0091] In an optional example, at least one surface of the box 101 is designed with an identification tag 120, including one of a barcode tag, a QR code tag, or an RFID tag, carrying unique coded information to mark and define the unique identity ID of each kit, enabling it to be identified, controlled, and traced by testing equipment or other equipment.

[0092] In an optional example, the housing 101 is also provided with at least one overflow port, which communicates with the cavity 110, to maintain a constant volume of incoming water sample within the reagent kit 100, ensuring testing standards and consistency.

[0093] {Detection reagent layer 102}

[0094] In an embodiment of the present invention, the aforementioned detection reagent layer 102 is preferably a melamine freeze-dried layer, especially a porous membrane structure formed by in-situ freeze-drying of the melamine reagent composition in the cavity 110, which is attached to at least one inner wall surface of the cavity 110. After contacting the water sample, it can quickly dissolve and react with cyanuric acid in the water sample in a short time, thereby forming a stable turbidity signal.

[0095] As an optional embodiment, the melamine reagent composition comprises the following components in parts by weight:

[0096] 10-60 parts of melamine powder;

[0097] 25-80 parts of hydrophilic framework material;

[0098] 0.001 to 1 part of Melamine-Cyanurate seed crystals;

[0099] Dispersant 0.1-10 parts;

[0100] 0-5 parts of auxiliary agents;

[0101] The auxiliary agents include at least one of a masking agent, a pH adjuster, and a stabilizer; the melamine reagent composition has a porous membrane structure with a water content of <1%.

[0102] As an optional implementation, the hydrophilic skeleton material is at least one of sugar alcohols, polysaccharides, or hydrophilic polymers. For example, at least one of mannitol, trehalose, sorbitol, polyvinylpyrrolidone, and polyethylene glycol may be used.

[0103] In this example, mannitol is used as an example of a hydrophilic framework material.

[0104] As an optional implementation, the dispersant is at least one of PVP, SDS, and Tween surfactants, in a weight ratio of 0.5 to 5 parts.

[0105] In this example, PVP K30 is used as the dispersant for illustration.

[0106] As an optional implementation, the aforementioned masking agent and stabilizer are present in parts by weight of 0 to 0.5 parts; the pH adjuster is present in parts by weight of 0 to 5 parts.

[0107] As an optional implementation, the pH adjuster is a phosphate buffer salt with pH=7.

[0108] As an optional implementation, the stabilizer may be a cellulose-based or a natural / synthetic polymer stabilizer. In this example, Triton X-100 or Tween 20 are used as examples of stabilizers.

[0109] As an optional implementation, the masking agent can be an inorganic salt masking agent or an organic masking agent, such as fluorides, inorganic phosphates, thiocyanates, HEDP, ATMP, EDTA, DTPA, citric acid, etc. In this example, EDTA-Na2 is used as an example of the masking agent.

[0110] It should be understood that in the embodiments of the present invention, if there are multiple types of components, the number of parts of a single substance is not limited, as long as the total content is within the required range.

[0111] The detection reagent layer 102 (melamine freeze-dried layer) prepared by the present invention dissolves and reacts within 5 to 60 seconds after contact with the water sample entering the cavity 110. The median particle size of the precipitate particles formed by the reaction is 1 μm to 10 μm, and the turbidity change is less than ±5% within 30 to 60 seconds after contact.

[0112] According to the method of the present invention, the preparation method of the aforementioned lyophilized reagent includes the following steps:

[0113] Melamine powder is pulverized to obtain melamine micro powder with particle size D50 < 5 μm and D90 < 10 μm.

[0114] According to the formula, the hydrophilic framework material, Melamine-Cyanurate seed particles, dispersant and auxiliary agents are added to the solvent and stirred until completely dissolved to obtain the matrix dispersion.

[0115] Melamine micro powder was added to the matrix dispersion and dispersed and homogenized to obtain a uniform and stable highly dispersed liquid composition.

[0116] The highly dispersed liquid composition is quantitatively injected into the reagent kit cavity according to a preset volume. The dispensed reagent kit cavity is then pre-frozen to allow the highly dispersed liquid composition to completely solidify and form an ice crystal structure.

[0117] The pre-frozen water quality testing chamber was placed in a vacuum environment for multi-stage temperature-controlled drying, forming a porous membrane structure with a water content of <1% in situ, thus obtaining the freeze-dried reagent.

[0118] Therefore, by lyophilizing in situ within the cavity of the reagent kit, the porous membrane layer naturally conforms to and adheres tightly to the inner wall and bottom of the cavity during the solidification and sublimation process of the lyophilized solution, forming a lyophilized layer with a microscopic fixation effect. This ensures that the reagent layer will not loosen or break during transportation, storage, or in environments where the reagent kit moves in a pool robot, thus ensuring the uniformity of reagent distribution before sampling.

[0119] In some embodiments, the specific process of pre-freezing includes: placing the dispensed water quality testing chamber in a freezing environment of -40 ℃ for 2 h.

[0120] In some embodiments, the specific process of multi-stage temperature-controlled drying under vacuum includes:

[0121] First drying stage: control the vacuum degree at 80 mTorr~100 mTorr, raise the temperature of the heating plate from -40 ℃ to -20 ℃, and maintain drying for 10 h~12 h;

[0122] Secondary drying stage: Control the vacuum degree to 50 mTorr~80 mTorr, continue to raise the plate temperature from -20 ℃ to 25 ℃, and maintain drying for 5 h~6 h.

[0123] Thus, through multi-stage vacuum drying control, a porous freeze-dried membrane layer that does not collapse or clump is formed.

[0124] In some embodiments, the pulverization process is carried out using an air jet mill, with the pulverization pressure controlled at 0.5 MPa to 0.7 MPa and the pulverization time at 15 min.

[0125] In some embodiments, ultrapure water is used as the solvent. It is understood that the volume of ultrapure water is not limited, and it is only used as a dissolving solvent.

[0126] In some embodiments, the dispersion and homogenization process includes: first, high-speed dispersion and stirring at a speed of 1000 rpm for 20 min, followed by ultrasonic treatment at a power of 200 W for 5 min.

[0127] In some embodiments, a highly dispersed liquid composition is quantitatively injected into the water quality detection chamber according to a preset volume of 100 μL ± 1 μL per test unit. This process must be carried out in a clean room, and the ambient temperature is controlled at 20 ℃ to 25 ℃, and the relative humidity is <40%.

[0128] To facilitate better understanding, the present invention will be further illustrated below with several specific examples, but the preparation process is not limited to these examples, and the content of the present invention is not limited to these examples.

[0129] Unless otherwise specified, the following embodiments are all conventional methods.

[0130] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.

[0131] All reagents used in the following examples are analytical grade, melamine powder purity is ≥99%, ultrapure water specification is 18.2 MΩ, and phosphate buffer pH=7.0.

[0132] Example 1

[0133] Melamine powder: 30 parts, mannitol: 55 parts, PVP: 2 parts, EDTA-Na2: 0.5 parts, Melamine-Cyanurate seed crystals: 0.05 parts.

[0134] Melamine powder was pulverized using an air jet mill, with the pulverization pressure controlled at 0.5 MPa to 0.7 MPa and the pulverization time at 15 min, to obtain melamine micro powder with D50 < 5 μm and D90 < 10 μm.

[0135] According to the formula, mannitol, Melamine-Cyanurate seed particles, PVP and EDTA were added to the solvent and magnetically stirred at 500 rpm for 10 min to completely dissolve them, thus obtaining a matrix dispersion.

[0136] Melamine micro powder was added to the matrix dispersion, and the mixture was first dispersed and stirred at high speed at 1000 rpm for 20 min, and then ultrasonically treated at 200 W for 5 min to obtain a uniform and stable highly dispersed liquid composition.

[0137] Using an automatic dispensing machine, the highly dispersed liquid composition was precisely injected into the detection chamber at a dispensing volume of 100 μL ± 1 μL per test unit. The dispensed water quality detection chamber was then placed in a -40 ℃ freezing environment for 2 h to allow the highly dispersed liquid composition to completely solidify and form an ice crystal structure.

[0138] The pre-frozen water quality testing chamber was transferred into a freeze dryer for staged sublimation drying and analytical drying:

[0139] First drying stage: The vacuum degree is controlled at 80 mTorr, the temperature of the heating plate is raised from -40 ℃ to -20 ℃, and the drying is maintained for 10 h to remove free moisture;

[0140] Secondary drying stage: Control the vacuum degree to 50 mTorr, continue to raise the plate temperature from -20 ℃ to 25 ℃, and maintain drying for 5 h to make the final porous membrane structure have a water content of <1%.

[0141] Example 2

[0142] The difference from Example 1 is that the components are: melamine powder: 60 parts, mannitol: 80 parts, PVP: 10 parts, EDTA-Na2: 0.25 parts, Triton X-100: 0.25 parts, phosphate buffer salt: 4.5 parts; Melamine-Cyanurate seed crystals: 1 part.

[0143] Example 3

[0144] The difference from Example 1 is that the components are: melamine powder: 10 parts, mannitol: 25 parts, PVP: 0.5 parts; Melamine-Cyanurate seed crystals: 0.05 parts.

[0145] Comparative Example 1

[0146] Melamine powder was pulverized using an air jet mill, with the pulverization pressure controlled at 0.5 MPa to 0.7 MPa and the pulverization time at 15 min, to obtain melamine micro powder with D50 < 5 μm and D90 < 10 μm.

[0147] Comparative Example 2

[0148] The difference from Example 1 is that the PVP content is increased to 3 parts, but no Melamine-Cyanurate seed crystals are included.

[0149] Dynamics and linear comparison test

[0150] To demonstrate the substantial technological advancements of the lyophilized reagent and its detection system in terms of reaction kinetics, dissolution rate, and detection accuracy, the following comparative and verification experiments were conducted:

[0151] (1) Preparation of standard samples of cyanuric acid and melamine

[0152] Preparation of cyanuric acid stock solution: Dissolve one trichloroisocyanuric acid effervescent tablet (approximately 500 ppm available chlorine) completely in 1 L of ultrapure water, allow to stand for 60 min to allow for decomposition and stabilization, and determine the actual reference concentration of cyanuric acid (CYA) using standard methods, which is 410 ppm. Subsequent water samples with concentrations of 20 ppm, 30 ppm, 35 ppm, 40 ppm, 45 ppm, 50 ppm, and 80 ppm were all obtained by precise dilution of this stock solution.

[0153] Preparation of melamine mother liquor: Accurately weigh 1.0 g of melamine micro powder (Comparative Example 1) and dissolve it in 1 L of ultrapure water to prepare a standard melamine solution with a concentration of 1 g / L.

[0154] (2) Effect of adding melamine alone on complexation reaction and solubility kinetics

[0155] To control the molar ratio of melamine to cyanuric acid in the water sample at 3:1, the following three comparative test methods were designed for direct addition of powder:

[0156] Method 1: Accurately measure 1 mL of each of the above-mentioned water samples of different concentrations of cyanuric acid, add a quantitative amount of melamine solution, heat in a 35 °C water bath for about 5 min to generate a suspension, and then immediately add 10 °C cold water to make up to 10 mL. After the forced high-energy reaction is completed, dilute the system 4 times and transfer it into a 90° scattering turbidimeter to measure the turbidity.

[0157] Method 2: Accurately measure a water sample of cyanuric acid with the same gradient and dilute it to 10 mL with room temperature water. Place it in a 35 °C water bath and preheat for a few minutes. Then, directly add a quantitative amount of melamine solution. After that, remove the water bath and allow it to naturally complex at room temperature (25 °C) without external stirring. After the reaction has been allowed to stand for 160 min, directly measure the 90° scattering turbidity of the system.

[0158] Method 3: Accurately measure a water sample of cyanuric acid of the same gradient, directly add it to a quantitative amount of melamine solution, then add room temperature water to make up to 10 mL, place the whole sample in a 35°C water bath and let it stand for 160 min (without mechanical stirring or shaking during the process), and then measure its 90° scattering turbidity.

[0159] The results are shown in Table 1 and Figure 7 As shown.

[0160] Table 1

[0161] As can be seen from the above, conventional melamine reacts very slowly due to its sluggish solubility kinetics, even when heated. However, when the concentration of 10 times that of cyanuric acid is used (i.e., water is added to make up the volume after the reaction), the reaction is much faster, but it will still be incomplete, resulting in non-ideal linearity.

[0162] (3) The scattering turbidity linearity and response of the lyophilized reagent of the present invention under different molar excess ratios.

[0163] Prepared according to the method in Example 1, cyanuric acid water samples of different concentrations were reacted with the lyophilization reagent, and the absolute content of melamine in the lyophilization reagent was adjusted to achieve a molar excess of 3:1, 5:1, 8:1, and 10:1 with cyanuric acid in the water sample in the reaction system. After standing at room temperature for 5 min, the intensity of scattered light was captured in real time through a 90° distributed light transmission window. The detection results are shown in Table 2 and... Figure 8 As shown.

[0164] Table 2

[0165] As can be seen from the above, compared with the experimental results of melamine alone in Table 1, the freeze-dried reagent layer of the present invention can completely and fully release the high-intensity scattering turbidity signal of water samples of 20 ppm to 80 ppm in only 5 minutes under normal temperature fluid conditions without the aid of external high-temperature heating and strong stirring. This proves the significant technical effect of the present invention in eliminating dissolution lag and accelerating complexation through a chemical equilibrium shift mechanism using a porous membrane layer.

[0166] Performance testing

[0167] Performance tests were conducted on Examples 1-3 and Comparative Examples 1-2, and the results are shown in Table 3.

[0168] Table 3

[0169] Test results show that the reagent reconstitution time of Example 1 of the present invention is <2 seconds, and the reagent reconstitution time of Example 2 is <1 second. Even in the examples with the lower limit of component content, the reconstitution time is < seconds, achieving ultra-fast dissolution at the second level. However, the reconstitution time of pure melamine powder in Comparative Example 1 is >60 seconds, which cannot meet the needs of rapid on-site detection.

[0170] The reagents in Examples 1-3 of this invention, after reconstitution, resulted in precipitates with a median particle size D50 locked within the ideal range of 1 μm to 10 μm, and more than 90% of the particles had a particle size distribution concentrated in the range of 1 μm to 10 μm, with no large-sized agglomerates. In contrast, the seedless formulation of Comparative Example 2 had a precipitate D50 of 8.7 μm, and the particle size distribution range expanded to 1 μm to 20 μm, showing obvious uncontrolled particle growth. Comparative Example 1, on the other hand, did not have a stable particle size distribution.

[0171] In Example 1 of this invention, the turbidity stabilization time of the reagent was <15 seconds, and the turbidity signal change rate within 60 seconds was ≤±3%; in Example 2, the turbidity stabilization time of the reagent was <10 seconds, and the turbidity signal change rate within 60 seconds was ≤±2%; even in Example 3, the turbidity signal change rate within 60 seconds was strictly controlled within ±5%, achieving ultra-stable output of optical signal within 60 seconds after contact with water sample; while in Comparative Example 1, the turbidity value continued to rise by more than 20% within 10 minutes, with no stable signal output, and the turbidity stabilization time of Comparative Example 2 was extended to 30 seconds, with a signal change rate within 60 seconds ≤±8%, and the stability was significantly lower than the effect of the embodiments of this invention.

[0172] Based on the above examples, the detection repeatability CV value of Example 1 of the present invention is <5%, and the detection repeatability CV value of Example 2 is <3%, both of which achieve high-precision quantitative detection of cyanuric acid; while the detection repeatability CV value of Comparative Example 1 is >20%, with no stable detection reproducibility, and cannot be used for quantitative detection at all.

[0173] Therefore, the lyophilized reagents prepared in Examples 1-3 of this invention all achieve the technical effects of rapid dissolution in seconds, precise control of precipitate particle size, and stable turbidity signal.

[0174] Therefore, the lyophilized reagent for cyanuric acid detection in swimming pools proposed in this invention, through the synergistic formulation of designed components and in-situ vacuum lyophilization, forms a porous membrane lyophilized structure with capillary water absorption effect in situ within the reagent kit. By combining the modified formulation with the porous physical morphology, the low-solubility core material melamine can be rapidly dissolved in a very short time (seconds) upon contact with the water sample. This not only eliminates the drawbacks of traditional solid reagents such as slow dissolution, particle growth, and response fluctuations, but also provides a high-concentration homogeneous substrate basis for subsequent uniform precipitation reactions, making it suitable for the needs of rapid fluid detection in automated equipment.

[0175] Meanwhile, the present invention designs and introduces trace amounts of Melamine-Cyanurate seed particles into the lyophilized reagent for pool cyanuric acid detection. These seed particles act as precipitation nuclei in the microscopic reaction system at the moment of rapid release from the porous lyophilized layer. Through the seed effect, the spontaneous and heterogeneous nucleation path of the traditional system is changed. Through the pre-nucleation induction mechanism and the synergistic inhibition effect of stabilizers and dispersants in the formulation, the median particle size of the subsequently generated precipitate particles is stabilized within the ideal microscopic range of 1 μm to 10 μm. This restricts the uncontrolled unlimited growth, aggregation, or flocculation of insoluble particles in the water sample, thereby achieving precise microscopic control of the precipitation process.

[0176] This invention overcomes the problem of continuous particle growth and severe time-dependent optical signal drift caused by uncontrolled kinetics in traditional reaction systems by modifying the formulation and combining it with a freeze-dried porous structure to ensure the consistency of the reaction starting point, while also using trace seed crystals to ensure the final particle size of the precipitation reaction. Through comprehensive kinetic control, the overall change rate of turbidity signal in the reaction system is suppressed to within ±5% within 30 to 60 seconds after contact with the water sample. The optical signal output is stable and has excellent reproducibility. This provides a highly accurate on-site quantitative analysis reagent that can quickly produce stable readings for swimming pool water quality testing robots or online intelligent water quality monitoring systems.

[0177] {Swimming Pool Water Quality Testing System}

[0178] Combination Figure 1 , Figure 2 , Figure 3 As shown, the swimming pool water quality testing system according to an embodiment of the present invention uses the aforementioned reagent kit 100 to test the swimming pool water quality, especially the content of cyanuric acid therein.

[0179] Combination Figure 3 The swimming pool water quality testing system shown in the example also includes a swimming pool water sample acquisition module 210, an optical detection module 220, a detection data processing module 230, and a control module 240.

[0180] Combination Figure 4 , 5 As shown, the reagent kit 100 can be externally coupled to the pool water sample collection module 210. At the coupling interface, the pool water sample collection module 210 can operate the water sample inlet 104 of the reagent kit 100, thereby forming a water sample collection channel inlet. For example, the water sample inlet 104 can be operated through a tube, a pointed conical tube opening, a pointed conical interface, or other components with hollow channels, so that the water sample collected by the pool water sample collection module 210 can enter the internal cavity of the reagent kit 100, thereby contacting and reacting with the detection reagent layer 102 inside the cavity 100, forming a suspension inside the cavity 110.

[0181] The control module 240 is connected to the pool water sample acquisition module 210, the optical detection module 220, and the detection data processing module 230, and:

[0182] The control pool water sample collection module 210 collects water from the pool and delivers it to the cavity 110 of the reagent kit 100;

[0183] The optical detection module 220 controls the emission of detection light signals through the light-transmitting window 103 of the reagent kit 100 into the suspension formed by the reaction within the cavity 110, and receives the light signals scattered back by the suspension; and

[0184] The control detection data processing module 230 calculates the concentration of cyanuric acid in the water sample based on the detection light signal, the returned light signal, and the calibration curve.

[0185] As an optional implementation, the control module 240 controls the pool water sample collection module 210 to deliver water samples into the reagent kit 100 according to a preset sampling volume, so as to maintain a constant water sample volume within the reagent kit 100. For example, water sample collection and delivery are performed according to a preset sampling flow rate and sampling time. As an optional example, the cavity 110 inside the reagent kit 100 serves as a reaction cavity, with a volume of 5 mL to 10 mL.

[0186] It should be understood that by providing an overflow port in the housing 101, the volume of the incoming water sample in the reagent kit 100 can be kept constant, ensuring testing standards and consistency.

[0187] As an optional implementation, the inner wall of the reagent kit 100 is also provided with a hydrophobic layer or a low surface energy coating to reduce liquid residue on the wall and ensure that the water sample volume is constant and accurate.

[0188] As a specific example, the aforementioned pool water sample collection module 210 includes a pump controlled by the control module 240, which can collect water samples from the pool from its intake side and operate the water sample inlet 104 from outside the reagent kit 100 from its outlet side, such as by piercing the diaphragm layer through a pointed conical tube, thereby forming a water sample collection channel inlet.

[0189] As an optional implementation, the optical detection module 220 includes a red LED for emitting detection light signals and a photosensitive PD for receiving the returned light signals.

[0190] Preferably, the red LED is an 850 nm wavelength LED emitter to avoid color interference, and the optical path is designed to be 2 mm to 8 mm to obtain sufficient scattering signal intensity.

[0191] As an optional method, a photosensitive PD is used as a detector, and its detection angle is set to a predetermined angle with the red LED, including but not limited to 90° (lateral scattering), 0°~90° (forward scattering, such as 25°, 45°), 90°~180° (backward scattering, such as 135°, 170°), etc.

[0192] In the example of this invention, 90° is taken as an example. The scattering turbidimetric method of sampling side scattering is used for detection, which reduces the influence of light source and particle size. The results have high comparability and repeatability, and are not sensitive to optical path and slight window contamination. The actual measurement shows that in melamine cyanurate (CYA) suspension, it can provide good signal intensity and linearity in the typical low to medium turbidity range of 30 ppm to 100 ppm in swimming pools.

[0193] In embodiments of the present invention, when a beam of light emitted by a red LED passes through a suspension containing melamine cyanurate precipitate particles, the particles scatter the incident light, and a definite quantitative relationship exists between the intensity of the scattered light and the number of particles per unit volume (i.e., the cyanuric acid concentration). In the melamine cyanurate system, the size of the precipitate particles generated by the reaction is typically on the order of micrometers (1-10 μm). In embodiments of the present invention, the detection principle is designed based on Michaelis-Menten's law:

[0194] I s =k·N·σ s ·I0;

[0195] Among them, I0, I s σ represents the incident light intensity and the scattered light intensity received by the detector, respectively; N represents the number of particles per unit volume of water sample; σ represents the incident light intensity and the scattered light intensity received by the detector, respectively. s The average scattering cross-sectional area of ​​a single particle is represented by k, which is the scattering coefficient. It is a geometric constant that is related to the incident light wavelength and the refractive index of the particle and the medium. It can be tested and calibrated by determining the incident light wavelength and the refractive index of the particle and the medium (water).

[0196] Therefore, the number of particles per unit volume of water sample is used as N as the target measurement value for detection. It is proportional to the cyanuric acid content (i.e., concentration). The larger N is, the higher the cyanuric acid content, the more precipitate particles are generated by the reaction with the detection reagents in the kit, and the higher the turbidity.

[0197] Therefore, under the conditions of constant water sample volume and constant detection reagent (melamine molar excess design), and under defined incident light conditions, k and σ s Both I and I0 are considered constants (known quantities), and the intensity of the scattered light I s It is directly proportional to the number of particles N in a unit volume of water sample:

[0198] I s∝ N∝C;

[0199] Where C represents the concentration of cyanuric acid.

[0200] like Figure 8 This example illustrates the test results for detecting cyanuric acid (CYA) in swimming pool water samples using the aforementioned kit. At room temperature, trichloroisocyanuric acid effervescent tablets were hydrolyzed to generate a cyanuric acid water sample with a concentration of 20 ppm to 80 ppm. The sample was then mixed using the kit described in the previous example, with a melamine molar excess of 3:1 or higher. After a reaction time of 2 to 3 minutes, the detected scattering turbidity was obtained for fitting and comparison experiments.

[0201] like Figure 8 In the experimental results shown, the horizontal axis represents the concentration of cyanuric acid, and the vertical axis represents the MCA scattering turbidity after the complexation reaction. The results indicate that cyanuric acid can be effectively detected at low ppm concentrations with an ideal linear relationship. Simultaneously, the visible I... s It is approximately linear with I0 and is insensitive to optical path length and slight window contamination.

[0202] Therefore, in the embodiments of the present invention, the detector output signal (scattered light intensity I) is established by the standard curve method. s The calibration curve between the concentration of cyanuric acid (C) and the concentration of cyanuric acid (C) is shown below. The procedure for establishing the standard curve is as follows:

[0203] (1) Prepare standard solutions of cyanuric acid of different concentrations: Prepare standard solutions of cyanuric acid of known concentrations with pure water to cover the target detection range; according to the typical range of cyanuric acid detection in swimming pool water is 10-100 ppm, the standard curve is usually configured with 7 or more concentration points.

[0204] (2) Complexation reaction: Add the same amount of detection reagent (the melamine detection reagent formulated in the above examples, especially the lyophilized reagent structure) to each standard solution, and the reaction generates melamine cyanurate precipitate to form a suspension;

[0205] (3) Optical detection: The intensity of scattered light of each standard solution is measured using an optical detection module of the same wavelength, and the output signal value is recorded;

[0206] (4) Fitting the standard curve: The concentration of the standard solution is plotted on the x-axis (C), and the intensity of the scattered light is plotted on the y-axis (I). s (or the digital value after analog-to-digital conversion), perform linear regression fitting to obtain the standard curve equation f(I) s ).

[0207] Simultaneously verify the linear correlation coefficient R. ² And the spiked recovery rate.

[0208] In another embodiment, higher-order polynomial fitting can be selected to obtain the standard curve equation, thereby improving the fitting and detection accuracy.

[0209] Furthermore, in an optional embodiment, the concentration of cyanuric acid in the water sample is calculated based on the standard curve and the intensity of scattered light output from the actual pool water sample test.

[0210] In an optional embodiment, the weak scattering signal (i.e., blank value) that may be generated by the pure water itself and the detection reagent is considered. Therefore, background removal can be performed within the detection system, using ultrapure water or blank reagent for measurement, recording the scattered light intensity as a zero point, and then subtracting this value from the actual sample measurement before calculation.

[0211] It should be understood that in the design of the detection system, the zero point can be preset during calibration or at the factory, and background subtraction compensation can be performed during detection, without the need to perform zero point measurement and calibration every time.

[0212] As an optional implementation, the pool water sample acquisition module 210, optical detection module 220, detection data processing module 230, and control module 240 are integrated into a detection device 200; combined with Figure 3 , Figure 4 , Figure 5 As shown, the reagent kit 100 can be coupled to the detection device 200 and connected to the pool water sample collection module 210.

[0213] Among them, the testing equipment 200 is a swimming pool robot.

[0214] It should be understood that, in the foregoing embodiments, the testing equipment 200, such as the pool robot, may be equipped with a coupling position for the reagent kit 100. This position may be designed to be located on the side of the equipment, at least partially embedded inside the testing equipment 200, or completely embedded inside the testing equipment 200. At these positions, a slot design compatible with the reagent kit 100 may be designed. After the reagent kit 100 is pushed in from the outside, it can be further secured in place by pre-clamping using latches, claws, or the like. In optional embodiments, a waterproof structure, including but not limited to elastic waterproof gaskets, may be further designed at the coupling interface and / or pushing position between the testing equipment 200 and the reagent kit 100.

[0215] The reagent kit 100 is detachably integrated into the detection device 200 and can be coupled to the pool water sample collection module 210.

[0216] In an embodiment of the present invention, the aforementioned calibration curve is set to be obtained by prior calibration testing under conditions of melamine and cyanuric acid in molar excess.

[0217] While the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the invention. Those skilled in the art can make various modifications and refinements without departing from the spirit and scope of the invention. Therefore, the scope of protection of the present invention shall be determined by the claims.

Claims

1. A kit for detecting cyanuric acid in swimming pools, characterized in that, include: The box body (101) has a closed cavity (110) inside, and a test reagent layer (102) is provided inside the cavity (110). A light-transmitting window (103) located on at least one surface of the housing; A water sample inlet (104) is provided on at least one surface of the housing (101) and allows water samples to enter the cavity (110) from the outside of the housing (101); The water sample inlet (104) is set to be normally closed, and the connection between the cavity (110) and the outside of the box (101) is isolated when not sampling.

2. The kit for detecting cyanuric acid in swimming pools according to claim 1, characterized in that, The number of light-transmitting windows (103) is two, located on two opposite surfaces of the box body, or on two surfaces distributed at 90°.

3. The kit for detecting cyanuric acid in swimming pools according to claim 1, characterized in that, The water sample inlet (104) is made of a membrane layer that can be punctured from the outside.

4. The kit for detecting cyanuric acid in swimming pools according to claim 3, characterized in that, The diaphragm layer is a thermoplastic polymer film layer.

5. The kit for detecting cyanuric acid in swimming pools according to claim 3, characterized in that, The diaphragm layer is a one-time sealed thin film layer that can be irreversibly ruptured under external mechanical puncture to form the entrance of the water sample collection channel.

6. The kit for detecting cyanuric acid in swimming pools according to claim 1, characterized in that, The water sample inlet (104) is made of a diaphragm layer with self-sealing channels.

7. The kit for detecting cyanuric acid in swimming pools according to claim 6, characterized in that, The diaphragm layer is made of an elastomer sheet, which keeps the self-sealing channel closed and elastically deforms in response to externally applied thrust, thereby opening the channel and forming the inlet of the water sample collection channel.

8. The kit for detecting cyanuric acid in swimming pools according to any one of claims 1 to 7, characterized in that, The box (101) is also provided with at least one overflow port, which is connected to the cavity (110).

9. The kit for detecting cyanuric acid in swimming pools according to claim 1, characterized in that, The test reagent layer (102) is a melamine freeze-dried layer, which is attached to at least one inner wall surface of the cavity (110).

10. The kit for detecting cyanuric acid in swimming pools according to claim 1, characterized in that, The melamine freeze-dried layer is a porous membrane layer formed by in-situ freeze-drying of the melamine reagent composition in the cavity (110).

11. The kit for detecting cyanuric acid in swimming pools according to claim 10, characterized in that, The melamine reagent composition comprises the following components in parts by weight: 10-60 parts of melamine powder; 25-80 parts of hydrophilic framework material; 0.001 to 1 part of Melamine-Cyanurate seed crystals; Dispersant 0.1-10 parts; 0-5 parts of auxiliary agents; The auxiliary agents include at least one of a masking agent, a pH adjuster, and a stabilizer; the melamine reagent composition has a porous membrane structure with a water content of <1%.

12. The kit for detecting cyanuric acid in swimming pools according to claim 11, characterized in that, The hydrophilic skeleton material is at least one of sugar alcohols, polysaccharides, or hydrophilic polymers.

13. The kit for detecting cyanuric acid in swimming pools according to claim 12, characterized in that, The hydrophilic skeleton material is at least one of mannitol, trehalose, sorbitol, polyvinylpyrrolidone, and polyethylene glycol.

14. The kit for detecting cyanuric acid in swimming pools according to claim 11, characterized in that, The dispersant is at least one of PVP, SDS, and Tween surfactants, in a weight ratio of 0.5 to 5 parts.

15. The kit for detecting cyanuric acid in swimming pools according to claim 11, characterized in that, The masking agent and stabilizer are present in parts by weight of 0 to 0.5 parts; the pH adjuster is present in parts by weight of 0 to 5 parts.

16. The kit for detecting cyanuric acid in swimming pools according to claim 11, characterized in that, The pH adjuster is a phosphate buffer salt with a pH of 7.

17. The kit for detecting cyanuric acid in swimming pools according to claim 11, characterized in that, The stabilizer is a cellulose-based stabilizer or a natural / synthetic polymer stabilizer.

18. The kit for detecting cyanuric acid in swimming pools according to claim 11, characterized in that, The masking agent is either an inorganic salt masking agent or an organic masking agent.

19. The kit for detecting cyanuric acid in swimming pools according to any one of claims 11 to 18, characterized in that, The test reagent layer (102) dissolves and reacts within 5 to 60 seconds after contact with the water sample entering the cavity (110). The median particle size of the precipitate particles formed by the reaction is 1 to 10 μm, and the turbidity change is less than ±5% within 30 to 60 seconds after contact.

20. A swimming pool water quality testing system, characterized in that, include: The kit (100) as described in any one of claims 1 to 19; Pool water sampling module (210), the reagent kit can be coupled to the pool water sampling module from the outside, at the coupling interface between the two, the pool water sampling module operates the water sample inlet (104) of the reagent kit to form a water sample sampling channel inlet; Optical inspection module (220); Detection data processing module (230); Control module (240); The control module (240) is connected to the pool water sample acquisition module (210), the optical detection module (220), and the detection data processing module (230), and: The pool water sample collection module (210) is controlled to collect water from the pool and deliver it to the cavity (110) of the reagent kit (100); The optical detection module (220) is controlled to emit detection light signals through the light-transmitting window (103) of the reagent kit (100) into the suspension formed by the reaction in the cavity (110), and to receive the light signals scattered back by the suspension. The detection data processing module (230) is controlled to calculate the concentration of cyanuric acid in the water sample based on the detection light signal, the returned light signal and the calibration curve.

21. The swimming pool water quality testing system according to claim 20, characterized in that, The control module (240) controls the pool water sample collection module (210) to deliver water samples into the reagent kit (100) according to a preset sampling amount, so as to keep the water sample volume in the reagent kit (100) constant.

22. The swimming pool water quality testing system according to claim 20, characterized in that, The inner wall surface of the cavity (110) inside the reagent kit (100) is provided with a hydrophobic layer or a low surface energy coating.

23. The swimming pool water quality testing system according to claim 20, characterized in that, The pool water sample collection module (210) includes a pump controlled by the control module (240), which can collect water samples from the pool from its intake side and can operate the water sample inlet (104) from outside the reagent kit (100) to form the water sample collection channel inlet.

24. The swimming pool water quality testing system according to claim 20, characterized in that, The optical detection module (220) includes a red LED for emitting detection light signals and a photosensitive PD for receiving returned light signals.

25. The swimming pool water quality testing system according to claim 24, characterized in that, The red LED is an LED emitter with a wavelength of 850nm.

26. The swimming pool water quality testing system according to claim 20, characterized in that, The pool water sample acquisition module (210), optical detection module (220), detection data processing module (230), and control module (240) are integrated into a detection device (200); The reagent kit (100) can be coupled to the detection device (200) and connected to the pool water sample collection module (210).

27. The swimming pool water quality testing system according to claim 26, characterized in that, The testing equipment (200) is a swimming pool robot.

28. A method for detecting cyanuric acid in swimming pools using a kit (100) as described in any one of claims 1 to 19, characterized in that, Includes the following steps: Operate the reagent kit (100) and establish the water sample collection channel entrance; A pool water sample is fed into the cavity (110) of the reagent kit (100) through the water sample collection channel inlet, so that the water sample comes into contact with the detection reagent layer (102) in the cavity (100) and reacts, forming a suspension in the cavity (110); After a certain reaction time, a detection light signal is emitted into the resulting suspension; Receive the optical signal returned after being scattered by the suspension; The concentration of cyanuric acid in the water sample is calculated based on the detected light signal, the returned light signal, and the calibration curve.

29. The method according to claim 28, characterized in that, The calibration curve was set to be obtained by pre-calibration testing under conditions of melamine and cyanuric acid in molar excess.

30. The method according to claim 28, characterized in that, The method utilizes a pool robot to achieve sampling and detection output.

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