A kind of moistening device and moistening method for microscopic analysis of fish larvae intestinal contents

Abstract

The present application relates to the technical field of fish larvae intestinal content research equipment, and particularly relates to a moistening device and method for microscopic analysis of fish larvae intestinal content. The device comprises a first connecting pipe connected with a liquid storage tank, a flow limiting mechanism having two channels, each of which is provided with a valve to control the two channels to be alternately conducted, a second connecting pipe connected with the flow limiting mechanism, a liquid injection mechanism having a cavity to receive the moistening liquid and output the liquid to another channel of the flow limiting mechanism, a liquid delivery pipe connected with the flow limiting mechanism and extending to an area above the glass slide, and a controller to control the quantitative liquid delivery. The present application can automatically add the moistening liquid, accurately control the amount of the added moistening liquid, effectively moisten the fish larvae, and realize customized observation according to the research object and the characteristics of the moistening liquid by setting the flow limiting mechanism and the liquid injection mechanism. In addition, the present application can effectively ensure the smoothness of the microscopic observation process, improve the convenience of the observation process, control the dosage of the moistening liquid, and reduce the consumption of reagents.

Description

Technical Field

[0001] This invention relates to the field of equipment technology for studying the intestinal contents of juvenile fish, and in particular to a humidification device and method for microscopic analysis of the intestinal contents of juvenile fish. Background Technology

[0002] Subtle changes in survival rates during the early life stages of fish can lead to significant interannual differences in population strength. Growth and survival during this stage have always been core issues in the study of fish replenishment mechanisms. Unsatisfactory growth in the larval stage is one of the main factors causing replenishment failure, and feeding conditions and feeding rates are important factors affecting larval growth. The "critical period hypothesis" posits that when larvae transition from endogenous to exogenous nutrition, many larvae die from starvation if food is scarce, but larval survival rates are high when food is plentiful.

[0003] Feeding status reflects the food supply in the environment of larvae and their ability to capture and ingest food. Analysis of intestinal filling and contents reveals the food availability and feeding activities of the larvae in their habitat before capture. Currently, the traditional method for analyzing larval feeding is visual observation of intestinal contents under a stereomicroscope. However, adding a humidifying solution during dissection can affect the smoothness and integrity of the process, causing numerous inconveniences. Furthermore, improper addition of the humidifying solution can also affect experimental procedures; specifically, too little humidifying solution will evaporate easily, causing the larvae to dry out, while too much will affect microscopic observation and cause significant analytical errors. Summary of the Invention

[0004] The present invention aims to at least improve one of the technical problems existing in the prior art. To this end, the present invention proposes a humidification device and method for microscopic analysis of the intestines of juvenile fish.

[0005] The technical solution of the present invention is as follows: A humidification device for microscopic analysis of juvenile fish intestines, comprising: Storage tank, used to hold humectant solution; The first connecting pipe is connected to the liquid storage tank; The flow limiting mechanism has two channels, one of which is connected to the first connecting pipe. Each channel is equipped with a valve to control the two channels to conduct alternately. The second connecting pipe is connected to the current limiting mechanism; The liquid injection mechanism has a cavity connected to the second connecting tube, which is used to receive the moisturizing liquid and output it to another channel of the flow limiting mechanism. The infusion tube has one end connected to another channel of the flow limiting mechanism and the other end extending to the area above the glass slide. A controller is installed on one side of the injection mechanism. The controller is connected to the valve and the injection mechanism, and is used to control the quantitative infusion.

[0006] In one possible technical solution, the injection mechanism further includes: frame; A sleeve is mounted on the frame and is connected to the second connecting pipe; The piston is installed inside the sleeve and is in close contact with the inner wall of the sleeve. It can translate along the axial direction of the sleeve. Piston rod, connected to the piston; A connecting block is installed at the end of the piston rod away from the piston; The drive rod is fixedly connected to the connecting block via a connecting rod. An electric cylinder is mounted on the frame. The output of the electric cylinder is connected to the drive rod. The electric cylinder is electrically connected to the controller. The controller can control the propulsion rate of the electric cylinder and precisely regulate the amount of moisturizing liquid added.

[0007] In one possible technical solution, it further includes: a first displacement sensor, installed on the sleeve sidewall near the second connecting pipe, the first displacement sensor being connected to the controller and used to collect the position information of the piston after the injection mechanism has finished injecting liquid; The second displacement sensor is installed on the side wall of the sleeve and located at two-thirds of the sleeve. The second displacement sensor is connected to the controller and is used to collect the position information of the piston after the liquid injection mechanism collects liquid.

[0008] In one possible technical solution, the end of the infusion tube near the glass slide is provided with an infusion hole for dripping liquid onto the glass slide to keep the fry moist.

[0009] In one possible technical solution, the channel further includes a first channel and a second channel, wherein: The first channel is composed of a first branch pipe and a connecting main pipe connected together. The first branch pipe is connected to the first connecting pipe and is used to deliver moisturizing liquid to the injection mechanism. The second channel consists of a second branch pipe and a connecting main pipe. The second branch pipe is connected to the infusion tube and is used to deliver the moisturizing solution to the glass slide.

[0010] In one possible technical solution, a further embodiment includes a tube clamp support, on which a tube clamp is ball-hinged for clamping the infusion tube to adjust the angle of the infusion tube. The height and lateral distance of the tube clamp can be manually adjusted to determine the position of the moisturizing solution droplet on the slide without affecting the field of view under the microscope.

[0011] In one possible technical solution, the valve is further described as an electric one-way flow valve, used to control the one-way flow of the moisturizing liquid.

[0012] In one possible technical solution, the first connecting pipe and the second connecting pipe have the same diameter, which facilitates the injection mechanism in controlling the inlet and outlet flow rates.

[0013] In one possible technical solution, the diameter of the infusion tube is further in the range of 2mm to 4mm, which facilitates the control of the infusion volume.

[0014] A method for humidifying the intestines of larvae under a microscope, wherein the larvae are humidified using the aforementioned humidification device, comprising: S1: Prepare a mixed solution containing 50% glycerin and 50% water as a moisturizing solution and add it to the storage tank; S2: Place the slide below the dissecting microscope objective; S3: Start the liquid injection mechanism to work. Only open the valve on the first channel. The controller controls the electric cylinder to pull the piston to start automatically filling the sleeve with moisturizing liquid. S4: Close the valve on the first channel, open the valve on the second channel, and the controller reverses the control of the electric cylinder to push the piston to start automatically injecting liquid into the third connecting tube, thereby dripping liquid onto the glass slide; S5: Place the fry on a glass slide, adjust the angle of the infusion tube using a clamp support, drip the fluid onto the glass slide, conduct continuous observation, dissect the intestines, and analyze the composition of the intestinal contents.

[0015] The humidification device and method for microscopic analysis of larval intestinal contents of the present invention can automatically add humidifying solution and precisely control the amount of humidifying solution added to achieve effective humidification of larvae, providing precise environmental control conditions for microscopic analysis of larval intestinal contents. Simultaneously, the flow-limiting mechanism and liquid injection mechanism can be customized according to the research object and the characteristics of the humidifying solution. Furthermore, the present invention effectively ensures a smooth microscopic observation process, improves the convenience of observation, controls the dosage of humidifying solution, reduces reagent consumption, ensures microscopic observation results, and shortens experimental time, among other significant features, making it of great importance for research on larval feeding analysis.

[0016] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the humidification device for microscopic analysis of the intestine contents of juvenile fish according to an embodiment of the present invention; Figure 2 This is a schematic diagram of the internal structure of the flow-limiting mechanism of the humidification device for objective microscopic analysis of juvenile fish intestines according to an embodiment of the present invention; Figure 3 This is a schematic diagram of the sleeve of a humidification device for microscopic analysis of the intestine contents of juvenile fish according to an embodiment of the present invention; Figure 4 This is a schematic cross-sectional view of the sleeve of a humidification device for microscope analysis of juvenile fish intestines according to an embodiment of the present invention; Figure 5 yes Figure 4 Enlarged schematic diagram of part A; Figure 6 yes Figure 4 Enlarged schematic diagram of part B.

[0019] Figure label: 1. Storage tank; 2. First connecting pipe; 3. Flow limiting mechanism; 30. Valve; 31. First branch pipe; 32. Second branch pipe; 33. Main connecting pipe; 4. Second connecting pipe; 5. Injection mechanism; 5. Frame; 501. Sleeve; 502. Piston; 503. Piston rod; 504. Connecting block; 505. Drive rod; 506. Electric cylinder; 507. First displacement sensor; 508. Second displacement sensor; 509. Infusion tube; 6. Glass slide; 7. Controller; 8. Detailed Implementation

[0020] The embodiments of the present invention are described in detail below. The embodiments described with reference to the accompanying drawings are exemplary. It should be understood that the specific embodiments described herein are only used to explain this application and are not intended to limit this application.

[0021] It should be noted that when a component is said to be "fixed to" another component, it can be directly attached to the other component or there may be an intervening component. When a component is said to be "connected to" another component, it can be directly connected to the other component or there may be an intervening component.

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0023] The terms "first," "second," "third," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish different objects and not to describe a particular order. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, it may include a series of steps or units, or optionally, steps or units not listed, or alternatively, other steps or units inherent to these processes, methods, products, or devices. (Computer Template) The accompanying drawings show only the portions relevant to this application, not all of them. Before discussing exemplary embodiments in more detail, it should be noted that some exemplary embodiments are described as processes or methods depicted as flowcharts. Although the flowcharts describe operations (or steps) as sequential processes, many of these operations may be performed in parallel, concurrently, or simultaneously. Furthermore, the order of the operations may be rearranged. The process may be terminated when its operation is completed, but may also have additional steps not included in the drawings. The process may correspond to a method, function, procedure, subroutine, subprogram, etc.

[0024] The terms “component,” “module,” “system,” “unit,” etc., used in this specification are used to refer to computer-related entities, hardware, firmware, combinations of hardware and software, software, or software in execution. For example, a unit can be, but is not limited to, a process running on a processor, a processor, an object, an executable file, a thread of execution, a program, and / or distributed between two or more computers. Furthermore, these units can be executed from various computer-readable media on which various data structures are stored. Units can communicate via local and / or remote processes based on signals having one or more data packets, such as data from a second unit interacting with another unit between a local system, a distributed system, and / or a network. For example, the Internet interacting with other systems via signals.

[0025] Example 1 like Figures 1 to 6 As shown, this embodiment provides a humidification device for microscopic analysis of juvenile fish intestines, comprising: Storage tank 1, used to hold humidifying liquid; The first connecting pipe 2 is connected to the liquid storage tank 1; The flow limiting mechanism 3 has two channels, one of which is connected to the first connecting pipe 2. Each channel is equipped with a valve 30 to control the two channels to be alternately open. The second connecting pipe 4 is connected to the flow limiting mechanism 3; The liquid injection mechanism 5 has a cavity connected to the second connecting tube 4, which is used to receive moisturizing liquid and output it to another channel of the flow limiting mechanism 3. The infusion tube 6 is connected at one end to another channel of the flow limiting mechanism 3, and at the other end extends to the area above the glass slide 7. The controller 8 is installed on one side of the injection mechanism 5. The controller 8 is connected to the valve 30 and the injection mechanism 5, and is used to control the quantitative infusion.

[0026] It should be noted that, in this embodiment, the liquid injection mechanism 5 includes: Rack 501; Sleeve 502 is mounted on the frame 501 and is connected to the second connecting pipe 4. Piston 503 is installed inside sleeve 502 and is in close contact with the inner wall of sleeve 502. It can translate along the axial direction of sleeve 502. Piston rod 504 is connected to piston 503; A connecting block 505 is installed at the end of the piston rod 504, away from the piston 503; The drive rod 506 is fixedly connected to the connecting block 505 via a connecting rod. An electric cylinder 507 is mounted on the frame 501. The output of the electric cylinder 507 is connected to the drive rod 506. The electric cylinder 507 is electrically connected to the controller 8. The controller can control the propulsion rate of the electric cylinder and precisely regulate the amount of moisturizing liquid added.

[0027] The first displacement sensor 508 is installed on the side wall of the sleeve 502 near the second connecting pipe 4. The first displacement sensor 508 is connected to the controller 8 and is used to collect the position information of the piston 503 after the liquid injection mechanism 5 has finished injecting liquid. The second displacement sensor 509 is installed on the side wall of the sleeve 502 and located at two-thirds of the sleeve 502. The second displacement sensor 509 is connected to the controller 8 and is used to collect the position information of the piston 503 after the liquid is collected by the liquid injection mechanism 5.

[0028] It should be noted that, in this embodiment, an infusion hole is provided at the end of the infusion tube 6 near the glass slide 7, which is used to drip liquid onto the glass slide to keep the fry moist.

[0029] It should be noted that, in this embodiment, the channel includes a first channel and a second channel, wherein: The first channel is composed of a first branch pipe 31 and a connecting main pipe 33. The first branch pipe 31 is connected to the first connecting pipe 2 and is used to deliver moisturizing liquid to the injection mechanism 5. The second channel is composed of a second branch pipe 32 and a connecting main pipe 33. The second branch pipe 32 is connected to the infusion tube 6 and is used to deliver the moisturizing solution to the glass slide 7.

[0030] It should be noted that, in this embodiment, the valve 30 is an electric one-way flow valve, used to control the one-way flow of the moisturizing liquid.

[0031] It should be noted that in this embodiment, the first connecting pipe 2 and the second connecting pipe 4 have the same diameter, which facilitates the injection mechanism 5 in controlling the inlet and outlet flow rates.

[0032] It should be noted that, in this embodiment, the diameter of the infusion tube 6 is in the range of 2mm to 4mm, which facilitates the control of the infusion volume of the infusion tube 6.

[0033] Example 2 like Figure 1 As shown, this embodiment further optimizes upon embodiment 1, providing a humidification device for microscopic analysis of juvenile fish intestines, comprising: Storage tank 1, used to hold humidifying liquid; The first connecting pipe 2 is connected to the liquid storage tank 1; The flow limiting mechanism 3 has two channels, one of which is connected to the first connecting pipe 2. Each channel is equipped with a valve 30 to control the two channels to be alternately open. The second connecting pipe 4 is connected to the flow limiting mechanism 3; The liquid injection mechanism 5 has a cavity connected to the second connecting tube 4, which is used to receive moisturizing liquid and output it to another channel of the flow limiting mechanism 3. The infusion tube 6 is connected at one end to another channel of the flow limiting mechanism 3, and at the other end extends to the area above the glass slide 7. The controller 8 is installed on one side of the injection mechanism 5. The controller 8 is connected to the valve 30 and the injection mechanism 5, and is used to control the quantitative infusion.

[0034] The tube clamp support 9 has a tube clamp 91 ball-hinged on it for clamping the infusion tube 6 to adjust the angle of the infusion tube 6. The height and lateral distance of the tube clamp 91 can be manually adjusted to determine the position of the moisturizing solution droplet on the slide without affecting the field of view under the microscope.

[0035] It should be noted that, in this embodiment, the lateral distance of the pipe clamp 91 can be controlled by adjusting the telescopic rod, and the height of the pipe clamp 91 can be achieved by adjusting the height of the fastening bolt on the bracket.

[0036] Example 3 Feeding analysis methods for larvae are widely used in studies of feeding-growth relationships. The research process includes body length measurement, gut removal, and gut dissection. The current general procedure involves placing the intact larvae on a slide / culture dish before dissection, and manually adding pre-prepared humectants such as alcohol preservation solution, distilled water, or 50% glycerol-distilled water using a dropper to ensure sample integrity during the procedure. Then, the larvae are imaged using a digital camera system mounted on a stereomicroscope, and measurements are taken to a minimum of 0.1 mm.

[0037] The entire intestine is removed from each specimen using a dissecting needle. The intestines of juvenile fish are generally long and thin, located on the ventral side of the body, making them easy to remove without damaging the remaining body tissue. The intestinal contents are then combed out for identification, counting, and measurement.

[0038] During the dissection of individual larvae, it is necessary to measure body length under a microscope while maintaining imaging, and to perform intestinal removal and dissection under the microscope. However, if too little humectant is added, it will evaporate easily, causing the larvae to dry out; if too much is added, it will affect microscopic observation. At the same time, the inconvenience of adding humectant also affects the smoothness and completeness of the analysis process.

[0039] Therefore, designing an effective humidification device and its practical method for microscopic observation and analysis of larval intestinal contents has become a pressing technical problem in this field. Based on this, this embodiment proposes a humidification method for microscopic analysis of larval intestinal contents to solve the above problem. The method involves using the humidification device described in the above embodiment to humidify the larvae, including: S1: Prepare a mixed solution containing 50% glycerin and 50% water as a moisturizing solution and add it to the storage tank; S2: Place the slide below the dissecting microscope objective; S3: Start the liquid injection mechanism to work. Only open the valve on the first channel. The controller controls the electric cylinder to pull the piston to start automatically filling the sleeve with moisturizing liquid. S4: Close the valve on the first channel, open the valve on the second channel, and the controller reverses the control of the electric cylinder to push the piston to start automatically injecting liquid into the third connecting tube, thereby dripping liquid onto the glass slide; S5: Place the fry on a glass slide, adjust the angle of the infusion tube using a clamp support, drip the fluid onto the glass slide, conduct continuous observation, dissect the intestines, and analyze the composition of the intestinal contents.

[0040] In this embodiment, the following specific implementation example is proposed, including the following steps: Step 1: Prepare a mixed solution containing 50% glycerin and 50% water as a moisturizing solution, and add it to the storage tank. The volume of the moisturizing solution should be 50mL. Step 2: Connect the humidification device for analyzing the larval intestines under the objective lens, and place the slide under the dissecting microscope objective lens; Step 3: Open the valve on the first channel of the flow limiting mechanism, turn on the controller to control the electric cylinder to pull the piston to start automatically filling the sleeve with moisturizing liquid. The sleeve has a capacity of 15mL, and the flow rate of the moisturizing liquid is controlled at 25mL / min, which can be adjusted by the controller. After the extraction is completed, close the valve on the first channel. Step 4: Open the valve on the second channel of the flow limiting mechanism. The controller reverses the control of the electric cylinder to push the piston to start automatically injecting liquid into the third connecting tube, thereby dripping liquid onto the glass slide. When injecting liquid, adjust the flow rate to 0.1 mL / min as needed. After the dripping is complete, close the valve on the second channel. Step 5: Adjust the angle of the infusion tube end to determine the position where the moisturizing solution will fall onto the slide without affecting the field of view under the microscope; Step Six: Place the fry under observation on a glass slide, adjust the angle of the infusion tube using the tube clamp support, drip the liquid onto the appropriate position on the glass slide, conduct continuous observation, dissect the intestine and analyze the composition of the intestinal contents.

[0041] It should be noted that, in this embodiment, the injection mechanism may also employ a precision injection pump and a syringe. The flow rate of the moisturizing solution can be observed by pushing the syringe forward or pulling it back at a constant speed using the precision injection pump.

[0042] The humidification device and method for microscopic analysis of larval intestinal contents of the present invention can automatically add humidifying solution and precisely control the amount of humidifying solution added to achieve effective humidification of larvae, providing precise environmental control conditions for microscopic analysis of larval intestinal contents. Simultaneously, the flow-limiting mechanism and liquid injection mechanism can be customized according to the research object and the characteristics of the humidifying solution. Furthermore, the present invention effectively ensures a smooth microscopic observation process, improves the convenience of observation, controls the dosage of humidifying solution, reduces reagent consumption, ensures microscopic observation results, and shortens experimental time, among other significant features, making it of great importance for research on larval feeding analysis.

[0043] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention.

[0044] In the description of this specification, references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example.

[0045] Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. The reference to "embodiment" herein means that a specific feature, structure, or characteristic described in connection with an embodiment can be included in at least one embodiment of this application. The appearance of this phrase in various places in the specification does not necessarily indicate the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0046] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A humidification device for microscopic analysis of juvenile fish intestines, characterized in that, include: Storage tank (1); The first connecting pipe (2) is connected to the liquid storage tank (1); The flow limiting mechanism (3) has two channels, one of which is connected to the first connecting pipe (2), and each channel is equipped with a valve (30) to control the two channels to alternately conduct; The second connecting pipe (4) is connected to the flow limiting mechanism (3); The liquid injection mechanism (5) has a cavity connected to the second connecting tube (4) for receiving moisturizing liquid and outputting it to another channel of the flow limiting mechanism (3); The infusion tube (6) is connected at one end to another channel and at the other end extends to the area above the glass slide (7); The controller (8) is installed on one side of the injection mechanism (5), and the controller (8) is connected to the valve (30) and the injection mechanism (5).

2. The humidification device for microscopic analysis of juvenile fish intestines according to claim 1, characterized in that, The liquid injection mechanism (5) includes: Rack (501); A sleeve (502) is installed on the frame (501), and the sleeve (502) is connected to the second connecting pipe (4); The piston (503) is installed inside the sleeve (502) and is in close contact with the inner wall of the sleeve (502). It can translate along the axial direction of the sleeve (502). Piston rod (504) is connected to piston (503); A connecting block (505) is installed at the end of the piston rod (504) away from the piston (503); The drive rod (506) is fixedly connected to the connecting block (505) via a connecting rod; An electric cylinder (507) is mounted on the frame (501). The output of the electric cylinder (507) is connected to the drive rod (506). The electric cylinder (507) is electrically connected to the controller (8).

3. The humidification device for microscopic analysis of juvenile fish intestines according to claim 2, characterized in that, Also includes: The first displacement sensor (508) is installed on the side wall of the sleeve (502) near the second connecting pipe (4) and connected to the controller (8); The second displacement sensor (509) is installed on the side wall of the sleeve (502) and located at two-thirds of the sleeve (502). The second displacement sensor (509) is connected to the controller (8).

4. The humidification device for microscopic analysis of juvenile fish intestines according to claim 1, characterized in that, An infusion hole is provided at the end of the infusion tube (6) near the glass slide (7).

5. The humidification device for microscopic analysis of juvenile fish intestines according to claim 1, characterized in that, The channel includes a first channel and a second channel, wherein: The first channel is composed of a first branch pipe (31) and a connecting main pipe (33), and the first branch pipe (31) is connected to the first connecting pipe (2); The second channel is composed of a second branch pipe (32) and a connecting main pipe (33), and the second branch pipe (32) is connected to the infusion tube (6).

6. The humidification device for microscopic analysis of juvenile fish intestines according to claim 1, characterized in that, The valve (30) is an electric one-way flow valve.

7. The humidification device for microscopic analysis of juvenile fish intestines according to claim 1, characterized in that, The first connecting pipe (2) has the same diameter as the second connecting pipe (4).

8. The humidification device for microscopic analysis of juvenile fish intestines according to claim 5, characterized in that, The diameter of the infusion tube (6) ranges from 2 mm to 4 mm.

9. The humidification device for microscopic analysis of juvenile fish intestines according to claim 1, characterized in that, Also includes: The tube clamp support (9) is ball-jointed with a tube clamp (91) for holding the infusion tube.

10. A method for moisturizing the contents of juvenile fish intestines under a microscope for analysis, characterized in that... The method of moisturizing fry using the moisturizing device as described in any one of claims 1 to 9 includes: S1: Prepare a mixed solution containing 50% glycerin and 50% water as a moisturizing solution and add it to the storage tank; S2: Place the slide below the dissecting microscope objective; S3: Start the liquid injection mechanism to work. Only open the valve on the first channel. The controller controls the electric cylinder to pull the piston to start automatically filling the sleeve with moisturizing liquid. S4: Close the valve on the first channel, open the valve on the second channel, and the controller reverses the control of the electric cylinder to push the piston to start automatically injecting liquid into the third connecting tube, thereby dripping liquid onto the glass slide; S5: Place the fry on a glass slide, adjust the angle of the infusion tube using a clamp support, drip the fluid onto the glass slide, conduct continuous observation, dissect the intestines, and analyze the composition of the intestinal contents.

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