A gastric functional in-vitro replacement experimental device with high reduction degree and a use method thereof

Abstract

The present application relates to the technical field of stomach function experiment, in particular to a stomach function in-vitro replacement experiment device with higher reduction and a use method, the device comprises a base, a temperature control water tank connected to the base through a support for temperature control, an experimental stomach body for stomach function in-vitro replacement experiment is arranged in the temperature control water tank, swing members for assisting the reaction of test samples in the experimental stomach body are arranged on the front and back sides of the temperature control water tank, the support is connected with a pressurizing mechanism for applying pressure to the experimental stomach body through a buffer, a sealing cover for putting test samples and sliding open and closed to both sides is arranged on the top of the temperature control water tank, the problem that the stomach function in-vitro replacement experiment device in the prior art cannot more closely restore the real digestion process of human or animal stomach function, so that the actual digestion process of stomach function exhibited by the experiment is not accurate enough, so that the research on stomach function by the experiment is not real and effective enough, and there is a certain difference from the real stomach function digestion situation is solved.

Description

Technical Field

[0001] This invention relates to the field of gastric function testing technology, specifically to an in vitro gastric function substitution experimental device with high fidelity and its usage method. Background Technology

[0002] After humans or animals ingest food, it undergoes a complex digestive process. Today, research on the digestive system is a popular area of ​​study for scholars in many fields. Understanding the digestive process in the human gastrointestinal tract is crucial for the development of many food and pharmaceutical products. Numerous issues closely related to daily human health require systematic and detailed research on digestion. Undeniably, in vivo experiments most accurately reflect the actual situation when studying the digestion of ingested substances. However, in vivo experiments often suffer from long research cycles, high complexity, and individual animal variability. Furthermore, in vivo experiments have many limitations, such as ethical constraints, high economic costs, and high technical requirements. In contrast, in vitro experiments have advantages such as no ethical constraints, saving time and costs, good reproducibility, ease of operation and laboratory simulation, and reduced animal casualties in simulated in vivo experiments, making them increasingly widely used.

[0003] Patent CN110890008A discloses a static in vitro alternative experimental device for gastric function, comprising a functional frame, a temperature-controlled water jacket, and a gastric body assembly. A water bath pump and electric heater inside the functional frame are connected to flowing hot water in the temperature-controlled water jacket, which transfers heat to the gastric body assembly via a water bath bowl for heating. The temperature-controlled water jacket is connected to a water inlet / outlet pipe and a sealing valve. A heat-insulating protective cover is installed on the outer wall of the temperature-controlled water jacket. The upper end of the gastric body assembly resembles the lesser curvature of the stomach, and the lower end resembles the greater curvature, with the gastric cavity in between. The cardia-like area on the right and the pylorus-like area on the left both have sample caps with nitrogen inlets, oxygen outlets, and various probes. A heat-insulating pad and a diffusion tray are placed on the lesser curvature of the stomach to hold pressure weights. A stirring motor with a drive magnetic head drives a magnetic rotor within the greater curvature of the stomach to rotate and stir during the experiment. The beneficial effects of this invention are: this device can replace in vivo experiments, providing a convenient and rapid static in vitro alternative experiment for gastric function at low cost and high efficiency, meeting standardized scientific research and application requirements.

[0004] However, the simulation effect of the above-mentioned in vitro gastric function replacement experimental device is not good, and it cannot more realistically reproduce the digestive process of human or animal gastric function. As a result, the actual digestive process of gastric function shown is not accurate enough, which leads to an insufficient study of human or animal gastric function and a certain difference between the study and the real situation. Summary of the Invention

[0005] The purpose of this invention is to provide a highly accurate in vitro experimental device for replacing gastric function and its usage method. This device overcomes the problem that existing in vitro experimental devices for replacing gastric function cannot accurately reproduce the real digestive process of human or animal stomachs during experiments, resulting in an inaccurate representation of the actual digestive process and an unrealistic and effective study of gastric function, which differs from the actual digestive situation of stomach function.

[0006] To achieve the above objectives, the technical solution adopted by the present invention to solve its technical problem is as follows: A highly accurate in vitro experimental device for gastric function substitution and its usage method were designed. This device places the experimental stomach in a temperature-controlled water tank, maintaining it at the required constant temperature. A pressurization mechanism applies pressure, and a swinging component agitates the stomach, replicating the environment of gastric function in humans or animals as closely as possible. This minimizes the gap between the experiment and reality, making the experiment closer to actual conditions, improving the accuracy of the research, and ensuring its authenticity and validity. The specific scheme is as follows: A highly accurate in vitro experimental device for replacing gastric function includes a base and a temperature-controlled water tank connected to the base via a support for temperature control. The temperature-controlled water tank contains an experimental stomach for in vitro gastric function replacement experiments. Swinging components for assisting the reaction of test samples within the experimental stomach are respectively provided on the front and rear sides of the temperature-controlled water tank. The support is connected to a pressurizing mechanism for applying pressure to the experimental stomach via a buffer component. The pressurizing mechanism is located on the upper side of the temperature-controlled water tank and controls the top of the temperature-controlled water tank to have a sealing cover for placing test samples and sliding open and closed to both sides.

[0007] Preferably, the swinging component includes an arc-shaped slide rail and a support leg. The arc-shaped slide rail is connected to the front and rear sides of the top of the base through the support leg. The inner side of the arc-shaped slide rail is movably connected to the pressurizing mechanism. The lower side of the arc-shaped slide rail is connected to a power component through a planetary gear assembly.

[0008] Preferably, the power assembly includes a bidirectional output shaft servo motor and a transmission belt. The bidirectional output shaft servo motor is located at the center of the top of the base. The front and rear ends of the bidirectional output shaft servo motor are respectively connected to belt shafts, and the belt shafts are poweredly connected to the planetary gear assembly through the transmission belt.

[0009] Preferably, the planetary gear assembly is connected to the front and rear sides of the temperature-controlled water tank. The planetary gear assembly includes an internal gear, small planetary gears, and large planetary gears. The internal gear is mounted on the bracket. The large planetary gear is connected to the internal gear through three small planetary gears. A gear connecting shaft is connected to the middle of the large planetary gear. The gear connecting shaft passes through the lower side of the arc-shaped slide rail and is powered by the transmission belt.

[0010] Preferably, the pressurizing mechanism includes a functional support and a movable component. The functional support is connected to the top of the support via the buffer, and the functional support is movably connected to the arc-shaped slide rail via a guide rod. The movable component is disposed on the functional support.

[0011] Preferably, the active component includes a stepper motor, a lifting rod, and an extrusion member. The stepper motor is mounted on the top of the functional bracket, the lifting rod is connected to the functional bracket through the output end of the stepper motor, and the extrusion member is connected to the lower side of the functional bracket through a connecting rod assembly and is connected to the sealing cover of the temperature-controlled water tank.

[0012] Preferably, the upper, lower, left, and right walls of the temperature-controlled water tank are all heat-insulating structures capable of elastic deformation. The upper interface water supply pipe is provided on the top front side of the temperature-controlled water tank, and the lower interface water return pipe is provided on the bottom rear side of the temperature-controlled water tank. A heat-conducting wire temperature controller for maintaining the temperature inside the temperature-controlled water tank is also provided at the lower interface water return pipe.

[0013] Preferably, the sealing caps on both sides are provided with corresponding arc-shaped slots in the middle, and air guide tile columns connected to the experimental stomach are inserted into the arc-shaped slots.

[0014] Preferably, the lower side of the experimental stomach is a pylorus-like opening, and the upper side is a cardia-like opening. The gas guide tile column is connected to the cardia-like opening, and both the gas guide tile column and the pylorus-like opening are connected to a piston cover. The piston cover is provided with a detection probe extending into the experimental stomach, and the piston cover is also provided with a knob cover for controlling the gas inlet and outlet.

[0015] A highly accurate in vitro experimental device for replacing gastric function and its usage method include the following steps: S1. Prepare the test sample and place it into the experimental stomach. S2. Nitrogen gas is introduced into the experimental stomach to create an anaerobic environment. S3. The experimental stomach is placed into the temperature-controlled water tank by controlling the opening and closing of the sealing cover of the temperature-controlled water tank through the pressurization mechanism. S4. The water in the temperature-controlled water tank forms a closed loop through the upper interface water supply pipe and the lower interface water return pipe, and the experimental stomach is placed in a constant temperature environment in conjunction with the heat conduction wire temperature controller. S5. Pressure is applied to the wall of the temperature-controlled water tank by the extrusion component in the pressurization mechanism, and the pressure is transmitted to the experimental stomach through the water in the temperature-controlled water tank, so that a certain pressure is generated in the experimental stomach. S6. The swinging component will cause the temperature-controlled water tank to swing back and forth, which, together with the buffer component, makes the experimental stomach inside the temperature-controlled water tank shake back and forth. S7. The various parameters in the experimental stomach are monitored at any time using a detection probe, and the experimental results are obtained.

[0016] The beneficial effects of this invention are: 1. This invention places the experimental stomach body in a temperature-controlled water tank to maintain it at the required constant temperature. The temperature-controlled water tank can be opened and closed by a pressurization mechanism, allowing the test sample to be taken out for research and analysis at any time. It is simple to operate and convenient to use.

[0017] 2. This invention utilizes a pressurizing mechanism to apply pressure, simulating the pressure state of the stomach in a human or animal body. A swinging component causes the stomach to oscillate, ensuring thorough and uniform mixing of the experimental sample within the stomach to meet the required external conditions. This allows for a more accurate replication of the stomach's functional environment in a human or animal body, minimizing the gap between the experiment and reality, making the experiment closer to actual conditions, improving the precision of the research, and ensuring its sufficient authenticity and effectiveness.

[0018] 3. The temperature-controlled water tank of the present invention is connected to the bracket through a planetary gear assembly. The power component can drive the temperature-controlled water tank to swing along the arc-shaped slide rail inside the bracket. The buffer component plays a certain buffering role in the swinging process of the temperature-controlled water tank, so as to avoid the swinging of the temperature-controlled water tank appearing abrupt.

[0019] 4. The power component of this invention uses a bidirectional output shaft servo motor to provide power, which can drive the temperature-controlled water tank to swing from the front and rear sides, thereby improving the stability of the swing of the temperature-controlled water tank. This allows the experimental stomach to have a stable shaking environment that closely resembles the actual situation, thereby improving the reaction efficiency of the test sample in the experimental stomach. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 2 This is a front view of an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 3 This is a side view of an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 4 This is a schematic diagram of the pressurization mechanism in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 5 This is a diagram showing the connection relationship between the C-shaped stent and the compression member in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 6 This is a schematic diagram of the C-type scaffold in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention; Figure 7 This is a schematic diagram of the extrusion component in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 8 This is a diagram showing the connection relationship between the planetary gear assembly and the bidirectional output shaft servo motor in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 9 This is a schematic diagram of the installation of the planetary gear assembly in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 10 This is a diagram showing the connection relationship between the planetary gear assembly and the front of the temperature-controlled water tank in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 11 This is a diagram showing the connection relationship between the planetary gear assembly and the rear side of the temperature-controlled water tank in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 12 This is a schematic diagram of the swinging component in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 13 This is a schematic diagram of the temperature-controlled water tank in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 14 This is a side view of the temperature-controlled water tank in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 15 This is a cross-sectional schematic diagram of the temperature-controlled water tank in the in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 16 This is a diagram showing the connection relationship between the experimental stomach and the gas-conducting tile column in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 17 This is a schematic diagram of the experimental stomach in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 18 This is a schematic diagram of the gas-conducting tile column in an in vitro experimental device for replacing gastric function with high reproducibility according to the present invention. Figure 19 This is a diagram showing the connection relationship between the gas-conducting tile column and the detection probe in an in vitro experimental device for replacing gastric function with high fidelity according to the present invention. Figure 20 for Figure 19 Schematic diagram of the cross section at point AA; Figure 21 This is a schematic diagram showing the open state of the sealing cover of the temperature-controlled water tank in the in vitro experimental device for replacing gastric function with high fidelity according to the present invention.

[0021] In the diagram: 1-Base; 11-Bracket; 2-Bidirectional output shaft servo motor; 21-Belt shaft; 22-Transmission belt; 3-Pressure mechanism; 31-Bearing plate; 32-Stepper motor; 321-U-shaped insert; 322-Insert rod; 33-Connecting column; 34-C-shaped bracket; 341-Guide rod; 342-Modular groove; 35-Lifting rod; 351-Sliding sleeve; 36-First connecting rod; 37-Second connecting rod; 38-Extrusion part; 381-Sliding section; 382-Extrusion section; 4-Temperature-controlled water tank; 41-Upper interface water supply pipe; 42-Lower interface water return pipe; 43-Sealing cover; 5-Swing component; 51-Arc-shaped slide rail; 511-Sliding groove; 52-Connecting hole; 53-Support leg; 6-Experimental stomach body; 61-Pyloric sac-like opening; 62-Valveolar sac-like opening; 7-Internal gear; 71-Large planetary gear; 72-Gear connecting shaft; 73-Small planetary gear; 8-Air guide tile column; 81-Piston cover; 82-Knob cover; 83-Detection probe; 9-Buffer component; 10-Heating wire temperature controller. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments and accompanying drawings. The illustrative embodiments and descriptions of the present invention are only used to explain the present invention and are not intended to limit the present invention.

[0023] In the following description, numerous specific details are set forth in order to provide a thorough understanding of the invention. However, it will be apparent to those skilled in the art that these specific details are not necessary to practice the invention. In other embodiments, well-known structures, circuits, materials, or methods have not been specifically described in order to avoid obscuring the invention.

[0024] Throughout this specification, references to "an embodiment," "an example," or "an example" mean that a particular feature, structure, or characteristic described in connection with that embodiment or example is included in at least one embodiment of the invention. Therefore, the phrases "an embodiment," "an example," "an example," or "an example" appearing in various places throughout the specification do not necessarily refer to the same embodiment or example. Furthermore, specific features, structures, or characteristics can be combined in one or more embodiments or examples in any suitable combination and / or sub-combination. Moreover, those skilled in the art will understand that the illustrations provided herein are for illustrative purposes and are not necessarily drawn to scale. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0025] In the description of this invention, the terms "front", "rear", "left", "right", "up", "down", "vertical", "horizontal", "high", "low", "inner", and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the scope of protection of this invention.

[0026] like Figure 1-21 As shown, the present invention provides an in vitro experimental device for gastric function substitution with high reproducibility, including a base 1 and a temperature-controlled water tank 4 connected to the base 1 via a bracket 11 for temperature control. The temperature-controlled water tank 4 contains an experimental stomach 6 for in vitro gastric function substitution experiments. Swinging components 5 for assisting the reaction of test samples in the experimental stomach 6 are respectively provided on the front and rear sides of the temperature-controlled water tank 4. The bracket 11 is connected to a pressurizing mechanism 3 for applying pressure to the experimental stomach 6 via a buffer component 9. The pressurizing mechanism 3 is located on the upper side of the temperature-controlled water tank 4, and the top of the temperature-controlled water tank 4 is provided with a sealing cover 43 for placing test samples and sliding open and closed to both sides.

[0027] As an optimized technical solution of the present invention, the test sample is placed in the experimental stomach 6, and the sealing cover 43 on the top of the temperature-controlled water tank 4 is opened and closed by the pressurizing component 3. The experimental stomach 6 is placed in the temperature-controlled water tank 4, and the pressurizing mechanism 3 applies pressure to the temperature-controlled water tank 4. The pressure can then be applied to the experimental stomach 6 by the water in the temperature-controlled water tank 4 to meet the required pressure in the experimental stomach 6. In addition, the swinging component 5 can drive the temperature-controlled water tank 4 to swing, thereby causing the experimental stomach 6 in the temperature-controlled water tank 4 to shake. The reaction efficiency of the test sample in the experimental stomach 6 can be improved by shaking.

[0028] In the above scheme, the swing component 5 includes an arc-shaped slide rail 51 and a support leg 53. The arc-shaped slide rail 51 is connected to the front and rear sides of the top of the base 1 through the support leg 53. The inner side of the arc-shaped slide rail 51 is movably connected to the pressurizing mechanism 3. The lower side of the arc-shaped slide rail 51 is connected to a power component through a planetary gear assembly.

[0029] As an optimized technical solution of the present invention, the inner side of the arc-shaped slide rail 51 is provided with a sliding groove 511. Under the drive of the power component, the temperature-controlled water tank 4 is driven to slide back and forth in the sliding groove 511 through the planetary gear assembly, thereby realizing the swing of the temperature-controlled water tank 4.

[0030] In the above scheme, the power component includes a bidirectional output shaft servo motor 2 and a transmission belt 22. The bidirectional output shaft servo motor 2 is located at the top center of the base 1. The front and rear ends of the bidirectional output shaft servo motor 2 are respectively connected to belt shafts 21. The belt shafts 21 are powered by the planetary gear assembly through the transmission belt 22.

[0031] As an optimized technical solution of the present invention, the swing of the temperature-controlled water tank 4 is achieved by the forward and reverse rotation of the bidirectional output shaft servo motor 2. In turn, the bidirectional output shaft servo motor 2 can drive the belt shafts 21 on the front and rear sides to rotate, and then drive the planetary gear assembly to operate through the transmission belt 22.

[0032] In the above scheme, the planetary gear assembly is connected to the front and rear sides of the temperature-controlled water tank 4. The planetary gear assembly includes an internal gear 7, small planetary gears 73 and large planetary gears 71. The internal gear 7 is mounted on the bracket 11. The large planetary gear 71 is connected to the internal gear 7 through three small planetary gears 73. A gear connecting shaft 72 is connected to the middle of the large planetary gear 71. The gear connecting shaft 72 is rotatably connected to the connecting hole 52 on the lower side of the arc-shaped slide rail 51 and is poweredly connected to the transmission belt 22.

[0033] As an optimized technical solution of the present invention, the transmission belt 22 drives the large planetary gear 71 to rotate through the gear connecting shaft 72, and then drives the internal gear 7 to rotate through the small planetary gear 73. At that time, the internal gear 7 can drive the temperature-controlled water tank 4 to swing back and forth through the connection relationship between the internal gear 7 and the front and rear sides of the temperature-controlled water tank 4, so as to meet the shaking conditions of the experimental stomach 6 inside it.

[0034] In the above scheme, the pressurizing mechanism 3 includes a functional support and a movable component. The functional support is connected to the top of the support 11 through a buffer 9. The functional support is movably connected to the arc-shaped slide rail 51 through a guide rod 341. The movable component is set on the functional support.

[0035] As an optimized technical solution of the present invention, the functional support includes a support plate 31 and a C-shaped support 34. The four corners of the bottom of the support plate 31 are connected to the C-shaped supports 34 located on the front and rear sides of the support plate 31 by connecting columns 33. One end of the guide rod 341 is connected to the two outer ends of the C-shaped support 34, and the other end of the guide rod 341 is slidably connected to the sliding groove 511 inside the arc-shaped slide rail 51, which limits the swing trajectory of the temperature-controlled water tank 4. One end of the buffer 9 is hinged between the C-shaped supports 34, and the other end of the buffer 9 is hinged to the support 11, so that it can play a buffering role during the swing of the temperature-controlled water tank 4.

[0036] In the above scheme, the active components include a stepper motor 32, a lifting rod 35, and an extrusion component 38. The stepper motor 32 is installed on the top of the functional bracket, the lifting rod 35 is connected to the functional bracket through the output end of the stepper motor 32, and the extrusion component 38 is connected to the lower side of the functional bracket through a connecting rod assembly and is connected to the sealing cover 43 of the temperature-controlled water tank 4.

[0037] As an optimized technical solution of the present invention, the stepper motor 32 is installed at the top center of the support plate 3. The output end of the stepper motor 32 is connected to a U-shaped plug 321. The plug rods 322 at both ends of the U-shaped plug 321 pass through the top center of the C-shaped bracket 34 and are connected to the upper middle part of the lifting rod 35. This allows the lifting rod 35 to move up and down within the C-shaped bracket 34. The extrusion member 38 is movably connected to the bottom two sides of the C-shaped bracket 34 and is driven by the connecting rod assembly set on the C-shaped bracket 34. Thus, the lifting rod 35 can control the extrusion member 38 to complete the opening and closing or extrusion work of the top sealing cover 43 of the temperature-controlled water tank 4.

[0038] In the above scheme, the linkage assembly includes a first linkage 36 and a second linkage 37. The lifting rod 35 is movably connected to a sliding sleeve 351 at both ends. One end of the first linkage 36 is hinged to the outside of the sliding sleeve 351, and the other end of the first linkage 36 is hinged to the extrusion member 38. One end of the second linkage 37 is hinged to the inside of the sliding sleeve 351, and the other end of the second linkage 37 is hinged to the lower corner of the C-shaped bracket 34.

[0039] As an optimized technical solution of the present invention, movable grooves 342 are provided on both sides of the bottom of the C-shaped bracket 34. The extrusion member 38 includes a sliding section 381 arranged horizontally on the upper side and an extrusion section 382 arranged vertically on the lower side. The sliding section 381 is slidably connected in the movable groove 342, and the extrusion section 382 is connected to the sealing cover 43. Thus, the sealing cover 43 can be opened and closed during the sliding of the sliding section 381 in the movable groove 342, or the extrusion section 382 can apply a certain pressure to the experimental stomach 6 inside the temperature-controlled water tank 4 by extruding the outer wall of the temperature-controlled water tank 4.

[0040] In the above scheme, the upper, lower, left and right walls of the temperature-controlled water tank 4 are all heat-insulating structures that can undergo elastic deformation. The upper interface water supply pipe 41 is provided on the top front side of the temperature-controlled water tank 4, and the lower interface water return pipe 42 is provided on the bottom rear side of the temperature-controlled water tank 4. A heat-conducting wire temperature controller 10 for maintaining the temperature inside the temperature-controlled water tank 4 is also provided at the lower interface water return pipe 42.

[0041] As an optimized technical solution of the present invention, the elastically deformable structure of the temperature-controlled water tank 4 allows the applied pressure to be transferred to the experimental stomach 6 through the water inside the water tank after the temperature-controlled water tank 4 is compressed. Hot water is added to the temperature-controlled water tank 4 through the upper interface return water pipe 41 to heat the experimental stomach 6 inside the tank. The hot water in the temperature-controlled water tank 4 is dynamically circulated through the lower interface return water pipe 42. At the same time, the hot water in the temperature-controlled water tank 4 can be monitored and heated by the heat-conducting wire temperature controller 10 to ensure that the temperature of the gastric function in vitro replacement experimental device is always stably maintained within the required experimental temperature range.

[0042] In the above scheme, the middle of the sealing caps 43 on both sides is provided with corresponding arc-shaped slots 44, and the gas guide tile column 8 connected to the experimental stomach body 6 is inserted into the arc-shaped slots 44.

[0043] As an optimized technical solution of the present invention, the gas guide tile column 8 is inserted into the arc-shaped slot 44, which can prevent the sealing cover 43 from being misaligned during the pressurization process. At the same time, gas can be injected or discharged into the experimental stomach 6 through the gas guide tile column 8.

[0044] In the above scheme, the experimental stomach body 6 has a pylorus-like opening 61 on the lower side and a cardia-like opening 62 on the upper side. The gas guide tile column 8 is connected to the cardia-like opening 62. Both the gas guide tile column 8 and the pylorus-like opening 61 are connected to piston caps 81. The piston cap 81 is equipped with a detection probe 83 that extends into the experimental stomach body 6. The piston cap 81 is also equipped with a knob cap 82 for controlling the gas inlet and outlet.

[0045] As an optimized technical solution of the present invention, the knob cover 82 on the piston cover 81 of the pyloric vent 62 is opened, nitrogen gas is introduced into the experimental stomach 6 from the outside in, and then the knob cover 82 is tightened to isolate it from the outside. Then the pyloric vent 61 is opened to release the oxygen in the experimental stomach 6, which provides an anaerobic environment for the experimental stomach 6 by isolating it from the outside. This creates favorable conditions for the reaction of the test sample in the experimental stomach 6. The detection probe 83 can then detect various relevant parameters in the experimental stomach 6, thereby obtaining experimental results that are closer to the actual situation, ensuring the accuracy of the gastric function substitution experiment, and making the research data more realistic and effective.

[0046] A highly accurate in vitro experimental device for replacing gastric function and its usage method include the following steps: S1. Prepare the test sample and place it into the experimental stomach 6; S2. Nitrogen gas is introduced into the experimental stomach 6 and oxygen gas is released to create an anaerobic environment inside the experimental stomach 6, which facilitates the reaction of the test sample inside the experimental stomach 6 and also helps to accurately obtain experimental parameters. S3. The U-shaped insert 321 in the pressurizing mechanism 3 drives the C-shaped bracket 34 to move up and down, thereby controlling the opening and closing of the sealing cover 43 of the temperature-controlled water tank 4, and placing the experimental stomach 6 into the temperature-controlled water tank 4. S4. The water in the temperature-controlled water tank 4 is made into a closed loop through the upper interface water supply pipe 41 and the lower interface water return pipe 42, so that the experimental stomach 6 can be heated by water bath to provide the temperature required for digestion. In addition, the heat conduction wire temperature controller 10 is used to place the experimental stomach 6 in a constant temperature environment, so that the temperature of the experimental stomach 6 is stably maintained within the temperature range required by the experiment. S5. Pressure is applied to the wall of the temperature-controlled water tank 4 by the extrusion component 38 in the pressurization mechanism 3, and the pressure is transmitted to the experimental stomach 6 through the water in the temperature-controlled water tank 4, so that a certain pressure is generated in the experimental stomach 6, thereby restoring the internal pressure of the human or animal digestive system. S6. The swinging component 5 will drive the temperature-controlled water tank 4 to swing back and forth, which, together with the buffer component 9, will make the experimental stomach 6 inside the temperature-controlled water tank 4 shake back and forth, thereby shaking the test sample inside the experimental stomach 6 evenly and accelerating its reaction. S7. The various parameters inside the experimental stomach 6 are monitored at any time by the detection probe 83, thereby effectively controlling the experimental conditions and restoring the gastric functional environment of the human or animal as much as possible. This ensures the authenticity, validity and accuracy of the experimental data of the gastric function substitution experiment, and also helps to obtain experimental results.

[0047] Specific implementation examples of the present invention are as follows: In the in vitro replacement experiment of gastric function, the test sample is prepared according to the experimental requirements. The piston cover 81 is opened and the prepared test sample is put into the experimental stomach body 6. After closing the piston cover 81, the experimental stomach body 6 is placed in the temperature-controlled water tank 4. Nitrogen gas is injected into the experimental stomach body 6 through the pyloric vent 62 to separate the inside of the experimental stomach body 6 from the outside. Then, oxygen in the experimental stomach body 6 is released through the pyloric vent 61, so that an anaerobic environment is formed inside the experimental stomach body 6 to facilitate the reaction of the test sample inside the experimental stomach body 6, which is conducive to the accurate acquisition of experimental parameters.

[0048] Restart the pressurization mechanism 3. The output end of the stepper motor 32 drives the lifting rod 35 to move upward on the C-shaped bracket 34 through the insertion rod 322 of the U-shaped plug 321. Then, through the interaction of the first connecting rod 36 and the second connecting rod 37, the sliding section 381 of the extrusion piece 38 slides backward along the movable groove 342. Then, the extrusion section 382 pulls the sealing cover 43 on the top of the temperature-controlled water tank 4 to open. After the sealing cover 43 is opened, the experimental stomach 6 containing the test sample can be placed into the temperature-controlled water tank 4, and the stepper motor 32 is controlled to drive the sealing cover 43 to close and complete the sealing.

[0049] After the experimental stomach 6 is placed into the temperature-controlled water tank 4, hot water is added to the temperature-controlled water tank 4 through the upper interface water supply pipe 41. With the lower interface water return pipe 42, the temperature-controlled water tank 4 forms a flow loop, thereby providing the experimental stomach 6 with the temperature required for digestion in the form of water bath heating. The heat conduction wire temperature controller 10 can also detect and heat the hot water in the temperature-controlled water tank 4, so that the temperature of the experimental stomach 6 is stably maintained within the temperature range required by the experiment.

[0050] At that time, the pressurization mechanism 3 can be restarted. The output end of the stepper motor 32 drives the lifting rod 35 to move downward on the C-shaped bracket 34 through the insertion rod 322 of the U-shaped plug 321. Then, through the cooperation of the first connecting rod 36 and the second connecting rod 37, the sliding section 381 of the extrusion piece 38 is forced to slowly approach each other along the movable groove 342. Then, the extrusion section 382 will apply a certain pressure to the wall of the temperature-controlled water tank 4. The pressure applied to the wall of the temperature-controlled water tank 4 will be transmitted to the experimental stomach 6 through the hot water in the temperature-controlled water tank 4, which will then compress the experimental stomach 6 and generate a certain pressure inside the experimental stomach 6, which is beneficial to restore the internal pressure of the digestive system of the human or animal stomach.

[0051] Simultaneously, the shaking of the experimental stomach 6 can be achieved through the swinging component 5. That is, the bidirectional output shaft servo motor 2 drives the belt shaft 21 to rotate back and forth, which in turn drives the gear connecting shaft 72 to rotate back and forth through the transmission belt 22, which in turn drives the large planetary gear 71 to rotate back and forth. The large planetary gear 71 drives the internal gear 7 to rotate back and forth through the small planetary gear 73, which in turn drives the temperature-controlled water tank 4 to swing back and forth, which in turn causes the experimental stomach 6 in the temperature-controlled water tank 4 to shake back and forth, so as to shake the test sample in the experimental stomach 6 evenly and accelerate its reaction.

[0052] During the swinging process of the temperature-controlled water tank 4, the pressurizing mechanism 3 will also swing, which will then slide within the sliding groove 511 of the arc-shaped slide rail 51 via the guide rod 341 of the C-shaped bracket 34. This provides a sliding track for the swinging of the C-shaped bracket 34, improving its stability. The buffer 9 between the bracket 11 and the C-shaped bracket 34 plays a crucial role in buffering the swinging process of the temperature-controlled water tank 4, preventing the swinging of the temperature-controlled water tank 4 from being too stiff and unnatural, thus affecting the stable shaking of the experimental stomach 6. It also helps to reduce the wear and tear on the device during the swinging process, thereby improving the service life of the device.

[0053] Throughout the experiment, the detection probe 83 on the piston cover 81 can detect various parameters inside the experimental stomach 6 at any time, thereby effectively controlling the experimental conditions or obtaining experimental results. By replicating the gastric function environment of the human or animal as much as possible, the authenticity, validity, and accuracy of the experimental data of the gastric function substitution experiment can be guaranteed, thereby minimizing the difference between reality and experiment, making the research more accurate and effective.

[0054] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A highly accurate in vitro experimental apparatus for replacing gastric function, comprising a base (1) and a temperature-controlled water tank (4) connected to the base (1) via a support (11) for controlling the temperature, wherein the temperature-controlled water tank (4) contains an experimental stomach (6) for in vitro gastric function replacement experiments, characterized in that: The temperature-controlled water tank (4) is equipped with swinging components (5) on the front and rear sides to assist the reaction of the test sample in the experimental stomach (6). The support (11) is connected via a buffer (9) to a pressurizing mechanism (3) for applying pressure to the experimental stomach (6). The pressurizing mechanism (3) is located on the upper side of the temperature-controlled water tank (4), and the top of the temperature-controlled water tank (4) is equipped with a sealing cover (43) for placing test samples and sliding open and closed to both sides.

2. The in vitro experimental device for replacing gastric function with high fidelity according to claim 1, characterized in that: The swing component (5) includes an arc-shaped slide rail (51) and a support leg (53). The arc-shaped slide rail (51) is connected to the front and rear sides of the top of the base (1) through the support leg (53). The inner side of the arc-shaped slide rail (51) is movably connected to the pressurizing mechanism (3), and the lower side of the arc-shaped slide rail (51) is connected to a power component through a planetary gear assembly.

3. The in vitro experimental device for replacing gastric function with high fidelity according to claim 2, characterized in that: The power assembly includes a bidirectional output shaft servo motor (2) and a transmission belt (22), and the bidirectional output shaft servo motor (2) is located at the top center of the base (1); The bidirectional output shaft servo motor (2) is connected to belt shafts (21) at both ends. The belt shaft (21) is powered to the planetary gear assembly via the transmission belt (22).

4. The in vitro experimental device for replacing gastric function with high fidelity according to claim 3, characterized in that: The planetary gear assembly is connected to the front and rear sides of the temperature-controlled water tank (4). The planetary gear assembly includes an internal gear (7), a small planetary gear (73) and a large planetary gear (71). The internal gear (7) is mounted on the bracket (11). The large planetary gear (71) is connected to the internal gear (7) via three small planetary gears (73); The large planetary gear (71) is connected to a gear connecting shaft (72) in the middle. The gear connecting shaft (72) passes through the lower side of the arc-shaped slide rail (51) and is powered by the transmission belt (22).

5. The in vitro experimental device for replacing gastric function with high fidelity according to claim 2, characterized in that: The pressurizing mechanism (3) includes a functional support and a movable component, wherein the functional support is connected to the top of the support (11) via the buffer (9); The functional support is movably connected to the arc-shaped slide rail (51) via a guide rod (341); The active components are mounted on the functional support.

6. The in vitro experimental device for replacing gastric function with high fidelity according to claim 5, characterized in that: The active components include a stepper motor (32), a lifting rod (35), and an extrusion member (38), with the stepper motor (32) mounted on top of the functional support; The lifting rod (35) is connected to the functional bracket through the output end of the stepper motor (32); The extrusion member (38) is connected to the lower side of the functional support via a connecting rod assembly and is connected to the sealing cover (43) of the temperature-controlled water tank (4).

7. The in vitro experimental device for replacing gastric function with high fidelity according to claim 1, characterized in that: The temperature-controlled water tank (4) has an upper, lower, left, and right wall that is an insulated structure that can undergo elastic deformation. The temperature-controlled water tank (4) is provided with an upper interface water supply pipe (41) on the top front side. The temperature-controlled water tank (4) is provided with a lower interface return water pipe (42) at the bottom rear side, and a heat-conducting wire temperature controller (10) for maintaining the temperature inside the temperature-controlled water tank (4) is also provided at the lower interface return water pipe (42).

8. The in vitro experimental device for replacing gastric function with high fidelity according to claim 1, characterized in that: The sealing caps (43) on both sides are provided with corresponding arc-shaped slots (44) in the middle, and the gas guide tile column (8) connected to the experimental stomach (6) is inserted into the arc-shaped slots (44).

9. The in vitro experimental device for replacing gastric function with high fidelity according to claim 8, characterized in that: The experimental stomach body (6) has a pylorus-like structure (61) on the lower side and a cardia-like structure (62) on the upper side. The air guide plate column (8) is connected to the pyloric portal (62), and a piston cover (81) is connected to both the air guide plate column (8) and the pyloric portal (61). The piston cover (81) is provided with a detection probe (83) extending into the experimental stomach (6); the piston cover (81) is also provided with a knob cover (82) for controlling the gas inlet and outlet.

10. A highly accurate in vitro experimental apparatus for replacing gastric function and a method of using it, comprising the highly accurate in vitro experimental apparatus for replacing gastric function as described in any one of claims 1-9, characterized in that: Includes the following steps: S1. Prepare the test sample and place it into the experimental stomach (6); S2. Nitrogen gas is introduced into the experimental stomach (6) to create an anaerobic environment inside the experimental stomach (6); S3. The sealing cover (43) of the temperature-controlled water tank (4) is opened and closed by the pressurizing mechanism (3), and the experimental stomach (6) is placed into the temperature-controlled water tank (4); S4. The water in the temperature-controlled water tank (4) is made to form a closed loop through the upper interface water supply pipe (41) and the lower interface water return pipe (42), and the experimental stomach (6) is placed in a constant temperature environment in conjunction with the heat conduction wire temperature controller (10). S5. Pressure is applied to the wall of the temperature-controlled water tank (4) by the extrusion component (38) in the pressurization mechanism (3), and the water in the temperature-controlled water tank (4) is transferred to the experimental stomach (6), so that a certain pressure is generated in the experimental stomach (6); S6. The swinging component (5) will cause the temperature-controlled water tank (4) to swing back and forth, and together with the buffer component (9), the experimental stomach (6) inside the temperature-controlled water tank (4) will shake back and forth. S7. The various parameters inside the experimental stomach (6) are detected at any time by the detection probe (83), and the experimental results are obtained.

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