Hair and / or scalp composition, placental growth factor production promoter

Abstract

The present invention aims to provide a novel hair and / or scalp composition. The present invention provides a hair and / or scalp composition containing a cortex cinnamomi extract and a pericarpium citri reticulatae extract, and provides a placental growth factor production promoter containing a cortex cinnamomi extract and a pericarpium citri reticulatae extract.

Description

Technical Field

[0001] This invention relates to a composition for hair and / or scalp containing extracts of Phellodendron bark and / or Citrus reticulata peel. Background Technology

[0002] Placental growth factor (PlGF) is classified as a member of the vascular endothelial growth factor (VEGF) family. of Cytokines specifically bind to VEGF receptor-1 (Flt-1). Increased production of PlGF promotes hair elongation. Methods for promoting the production of placental growth factor in the skin by irradiating it with light of a specific wavelength are known (Patent Document 1).

[0003] It is known that hair-related formulations containing extracts such as Phellodendron amurense improve the spreadability of their preparations (Patent Document 2). It is also known that a scalp formulation contains Citrus reticulata extract as an ingredient that dilates scalp capillaries and can remove hardened sebum (Patent Document 3).

[0004] Although formulation improvements and developments to enhance the scalp environment are underway, there is a need for products that are effective in promoting hair growth.

[0005] [Existing technical documents]

[0006] [Patent Literature]

[0007] [Patent Document 1] Japanese Patent Application Publication No. 2021-171084

[0008] [Patent Document 2] Japanese Patent Application Publication No. 2020-055801

[0009] [Patent Document 3] Japanese Patent Application Publication No. 2014-208714 Summary of the Invention

[0010] [The problem the invention aims to solve]

[0011] The object of this invention is to provide a novel composition for hair and / or scalp. Furthermore, the object of this invention is to provide a novel placental growth factor production promoter.

[0012] [Methods used to solve problems]

[0013] The inventors have discovered that by preparing a composition containing extracts of Phellodendron bark and / or dried tangerine peel, the production of placental growth factor can be promoted. The invention is based on this insight.

[0014] [The effects of the invention]

[0015] The hair and / or scalp composition of the present invention has an excellent effect on promoting the production of placental growth factor because it contains Phellodendron bark extract and / or tangerine peel extract. Attached Figure Description

[0016] Figure 1 This indicates the placental growth factor-promoting effect brought about by extracts of Phellodendron bark or dried tangerine peel.

[0017] Figure 2 This indicates the placental growth factor-promoting effect brought about by extracts of Phellodendron bark and dried tangerine peel.

[0018] Figure 3 This indicates the effect of containing lime extract or berberine in promoting the production of placental growth factors. Detailed Implementation

[0019] The following describes in detail the methods for implementing the present invention. However, the present invention is not limited to the following embodiments. In this specification, the term "hair growth" includes the function of advantageously promoting hair development and growth. In particular, in addition to preventing or preventing the development of symptoms such as decreased elasticity and resilience of hair or eyelashes, reduced hair volume or volume on the top of the head or other areas, or thinning of hair, it also includes lengthening hair after hair growth, thickening hair after hair growth, or making hair less prone to falling out after hair growth. Through this effect, for example, it also helps to suppress undesirable hair curl.

[0020] Hair refers to any hair that grows on the body of animals, including humans. This includes hair, eyebrows, eyelashes, beard, chest hair, back hair, and all other body hair, but mainly refers to hair, eyebrows, or eyelashes.

[0021] [1. Compositions for hair and / or scalp]

[0022] The hair and / or scalp composition of this embodiment contains Phellodendron bark extract and / or tangerine peel extract. The hair and / or scalp composition of this embodiment contains lime extract, berberine and / or its salts, or combinations thereof.

[0023] Phellodendron bark refers to plants of the Rutaceae family. More specifically, it refers to plants of the genus *Phellodendron*, and in even more specific examples, it refers to *Phellodendron amurense*. The *Phellodendron bark* extract of this invention is preferably an extract from the bark. The extraction method or solvent for *Phellodendron bark* is not particularly limited, and known techniques can be used. The extract can be used directly in liquid form, or as a powder or granules obtained by drying the extract, or as a decomposition product obtained by processing the extract using known techniques such as enzymatic decomposition. Commercially available products can be used. Examples of commercially available *Phellodendron bark* extracts include: *Phellodendron bark extract B* (manufactured by ICHIMARUSHARCOS Co., Ltd.), *Phellodendron bark extract E* (manufactured by ICHIMARUSHARCOS Co., Ltd.), *Phellodendron bark extract* (manufactured by Kōei Kōyō Co., Ltd.), *Phellodendron bark extract BG* (manufactured by Kōei Kōyō Co., Ltd.), *Phellodendron bark extract-J* (manufactured by Maruzen Pharmaceutical Co., Ltd.), and *Phellodendron bark extract BG-J* (manufactured by Maruzen Pharmaceutical Co., Ltd.).

[0024] Dried tangerine peel refers to plants of the Rutaceae family. In a more specific example, dried tangerine peel refers to plants of the Citrus genus, and in an even more specific example, dried tangerine peel refers to Wenzhou mandarin oranges (Citrus unshiu). The dried tangerine peel extract of the present invention is preferably an extract of the peel. There are no particular limitations on the extraction method or extraction solvent of dried tangerine peel, and known techniques can be used. The extract can be used directly in liquid form, or it can be used as powder or granules obtained by drying the extract, or the extract can be treated with known techniques such as enzymatic decomposition and the resulting decomposition products can be used. Commercially available products can be used. Examples of commercially available dried tangerine peel extracts include: MandarinClear (manufactured by ICHIMARU PHARCOS Co., Ltd.), dried tangerine peel liquid (manufactured by ICHIMARU PHARCOS Co., Ltd.), dried tangerine peel extract BG (manufactured by Maruzen Pharmaceutical Co., Ltd.), dried tangerine peel extract-J (manufactured by Maruzen Pharmaceutical Co., Ltd.), and ism (Wenzhou mandarin orange) (manufactured by Maruzen Pharmaceutical Co., Ltd.), etc.

[0025] Lime (Citrus aurantium) refers to plants of the Rutaceae family. In a more specific example, lime refers to plants of the Citrus genus, and in an even more specific example, lime refers to Citrus aurantium. The lime extract of the present invention is preferably an extract from the peel. There are no particular limitations on the extraction method or extraction solvent of lime, and known techniques can be used. The extract can be used directly in liquid form, or it can be used as a powder or granules obtained by drying the extract, or the extract can be treated with known techniques such as enzymatic decomposition and the resulting decomposition products can be used. Commercially available products can be used. Examples of commercially available lime extracts include: lime extract (manufactured by Maruzen Pharmaceutical Co., Ltd.), lime liquid B (manufactured by ICHIMARU PHARCOS Co., Ltd.), etc.

[0026] The content of lime extract in the hair and / or scalp composition of this embodiment is not particularly limited as long as it is within the range that promotes the production of placental growth factor, and is appropriately set according to the type and content of other formulated ingredients, the formulation form of the hair and / or scalp composition, etc. From the viewpoint of more obviously exerting the effects of the present invention, for example, it is preferred to be 0.0001 to 5% by mass, more preferably 0.0001 to 4% by mass, further preferably 0.001 to 3% by mass, further preferably 0.01 to 1.5% by mass, and especially preferably 0.1 to 1% by mass.

[0027] Berberine refers to a known compound classified as a benzylisoquinoline alkaloid. There are no particular limitations on the salts of berberine, as long as they are pharmaceutically acceptable; examples include berberine chloride, berberine sulfate, and berberine tannate. The content of berberine and its salts in the hair and / or scalp composition of this embodiment is not particularly limited as long as it is within the range that promotes the production of placental growth factor. From the viewpoint of more significantly exerting the effects of the present invention, it is preferably 1.0 to 25 μmol / L, more preferably 1.0 to 20 μmol / L, even more preferably 1.6 to 16 μmol / L, even more preferably 2.0 to 14 μmol / L, and particularly preferably 3.0 to 12 μmol / L.

[0028] The hair and / or scalp composition of this embodiment has a placental growth factor-promoting effect, and is expected to promote hair growth. In particular, it is expected to promote hair elongation (e.g., make hair grow faster). Furthermore, the hair and / or scalp composition of this embodiment is also expected to make hair thicker. Therefore, in some embodiments, the hair and / or scalp composition of the present invention is a composition for promoting hair elongation or making hair thicker.

[0029] In some embodiments, compared to hair follicles without the added component, hair shaft elongation is increased in hair follicles with the added component, thereby confirming that hair elongation is promoted. For example, in hair follicles with the added component, elongation increases by more than 3 times, preferably more than 5 times, within 2 days compared to hair follicles without the added component. In other embodiments, for example, when hair follicles are cultured in a culture medium with the added component, hair elongation increases by more than 7%, preferably more than 10%, on the 8th day, relative to the hair elongation on the 2nd day.

[0030] In some embodiments, the hair thickness increases in hair follicles containing the added component compared to hair follicles without the component, thereby confirming the change in hair thickness. For example, in hair follicles containing the added component, the hair thickness increases by more than two times, preferably more than three times, within 4 days, and by more than five times, preferably more than seven times, within 8 days compared to hair follicles without the added component. In other embodiments, for example, when hair follicles are cultured in a culture medium containing the added component, the hair thickness increases by more than 1%, preferably more than 2%, on the fourth day relative to the hair thickness on the second day.

[0031] The content of Phellodendron amurense extract in the hair and / or scalp composition of this embodiment is not particularly limited as long as it is within the range that promotes the production of placental growth factor, and is appropriately set according to the type and content of other formulation ingredients, the formulation form of the hair and / or scalp composition, etc. From the viewpoint of more obviously exerting the effects of the present invention, for example, it is preferred to be 0.00001 to 5% by mass, more preferably 0.00001 to 3% by mass, further preferably 0.001 to 1% by mass, further preferably 0.01 to 0.5% by mass, and especially preferably 0.03 to 0.1% by mass.

[0032] The content of tangerine peel extract in the hair and / or scalp composition of this embodiment is not particularly limited as long as it is within the range that promotes the production of placental growth factor, and is appropriately set according to the type and content of other formulation ingredients, the formulation form of the hair and / or scalp composition, etc. From the viewpoint of more obviously exerting the effects of the present invention, for example, it is preferred to be 0.0001 to 5% by mass, more preferably 0.0001 to 4% by mass, further preferably 0.001 to 3% by mass, further preferably 0.01 to 1.5% by mass, and especially preferably 0.1 to 1% by mass.

[0033] From the viewpoint that the content ratio of tangerine peel extract to phellodendron bark extract in the hair and / or scalp composition of this embodiment can more obviously exert the effects of the present invention, for example, relative to 1 part by mass of the total content of phellodendron bark extract contained in the hair and / or scalp composition of this embodiment, the total content of tangerine peel extract is preferably 0.001 to 5000 parts by mass, more preferably 0.001 to 3000 parts by mass, and even more preferably 0.02 to 1500 parts by mass, and particularly preferably 1 to 100 parts by mass.

[0034] The hair and / or scalp composition of this embodiment may further contain excipients, thickeners, binders, disintegrants, dispersants, surfactants, suspending agents, emulsifiers, stabilizers, gelling agents, sweeteners, acidulants, umami agents, salty agents, bitter agents, colorants, UV protectants, antioxidants, preservatives, antibacterial agents, pH adjusters, buffers, chelating agents, vitamins, flavorings, minerals and other additives, amino acids or their salts, sugars, humectants, organic acids or their salts, dietary fiber, animal tissue extracts, etc. These may be appropriately selected and used by those known to the public, and can be manufactured using conventional methods.

[0035] The dosage form of the hair and / or scalp composition of this embodiment is not limited to a specific product or property, and can be appropriately prepared into any dosage form such as liquid, gel, cream, paste, solid, aerosol, or patch using known methods. Examples of liquids include lotions, emulsions, beauty serums, oral liquids, syrups, and hair growth agents. Examples of solids include sticks, tablets, chewable tablets, granules, powders, capsules, and ointments. Furthermore, regarding the administration method, from the viewpoint of maximizing the effects of the present invention, a topical application is preferred, regardless of whether it is a topical or internal application. Topical applications are not limited to pharmaceuticals, quasi-pharmaceuticals, or cosmetics, and are not limited thereto. Examples of administration methods include transdermal administration and subcutaneous injection. More specifically, examples of non-invasive administration methods include... Additionally, it can also be formulated as shampoo, conditioner, etc. These can be manufactured using conventional methods.

[0036] The hair and / or scalp composition of the present invention is not limited to the scalp and can be applied to hair and hairy parts thereof. For example, the composition of the present invention can be applied to the scalp, eyelashes, eyebrows, beard, or lips, etc.

[0037] [2. Placental growth factor production promoters]

[0038] The placental growth factor production promoter of this embodiment contains Phellodendron bark extract and / or tangerine peel extract.

[0039] In this embodiment of the placental growth factor production promoter, the content of Phellodendron bark extract and / or tangerine peel extract, the types and contents of other components, the formulation form and uses of the placental growth factor production promoter, etc., are as follows: [1. Hair] and / or As described in the composition for scalp use.

[0040] [3. Methods to promote placental growth factor production]

[0041] The hair and / or scalp composition of this embodiment promotes the production of placental growth factor by containing Phellodendron amurense extract and / or Citrus reticulata peel extract. Therefore, as one embodiment of the present invention, a method is provided that imparts the effect of promoting placental growth factor production to the hair and / or scalp composition containing Phellodendron amurense extract and / or Citrus reticulata peel extract. Furthermore, as another embodiment of the present invention, a method for promoting placental growth factor production is provided, comprising administering Phellodendron amurense extract and / or Citrus reticulata peel extract to a subject, including or not including a human subject.

[0042] Regarding the content of Phellodendron bark extract and / or tangerine peel extract in this embodiment, the types and contents of other ingredients, the formulation and uses, etc., such as [1. hair] and / or As described in the composition for scalp use.

[0043] [4. Methods to promote hair growth]

[0044] The hair and / or scalp composition of this embodiment has a placental growth factor-promoting effect, and is expected to promote hair growth. In particular, it is expected to promote hair elongation (e.g., make hair grow faster) or increase hair thickness. Therefore, as one embodiment of the present invention, a method is provided that includes a Phellodendron amurense extract and / or a dried tangerine peel extract to impart a hair elongation-promoting effect to the hair and / or scalp composition. As one embodiment of the present invention, a method is provided that includes a Phellodendron amurense extract and / or a dried tangerine peel extract to impart a hair thickness-promoting effect to the hair and / or scalp composition. As another embodiment of the present invention, a method for promoting hair elongation is provided, comprising administering a Phellodendron amurense extract and / or a dried tangerine peel extract to a subject, including or not including a human subject. As another embodiment of the present invention, a method for promoting hair thickness is provided, comprising administering a Phellodendron amurense extract and / or a dried tangerine peel extract to a subject, including or not including a human subject.

[0045] Regarding the content of Phellodendron bark extract and / or tangerine peel extract in this embodiment, the types and contents of other ingredients, the formulation and uses, etc., such as [1. hair] and / or As described in the composition for scalp use.

[0046] In this invention,

[0047] [1] A hair and / or scalp composition containing extracts of Phellodendron bark and / or Citrus reticulata peel is provided.

[0048] Preferred embodiments of the present invention include the following.

[0049] [2] The hair and / or scalp composition as described in [1] is used to promote the production of placental growth factor of hair papilla cells.

[0050] [3] A placental growth factor production promoter containing hair and / or scalp composition as described in [1] or [2].

[0051] [4] A placental growth factor production promoter containing Phellodendron bark extract and / or tangerine peel extract.

[0052] [5] A placental growth factor production promoter containing one or more of the following: a lime extract, berberine, and salts of berberine.

[0053] [6] A hair and / or scalp composition containing a placental growth factor production promoter as described in [5].

[0054] [7] A hair and / or scalp composition as described in [6], which is used to promote the production of placental growth factor of hair papilla cells.

[0055] [8] An oral composition containing a placental growth factor production promoter as described in [4] or [5].

[0056] [9] A method for promoting placental growth factor production, comprising Phellodendron bark extract and / or tangerine peel extract.

[0057]

[10] A method for manufacturing a placental growth factor production promoter, wherein a mixture of Phellodendron bark extract and / or tangerine peel extract is prepared.

[0058]

[11] A method for promoting the production of placental growth factor, comprising one or more ingredients selected from the group consisting of lime extract, berberine and salts of berberine.

[0059]

[12] A method for manufacturing a placental growth factor production promoter, comprising one or more ingredients selected from the group consisting of lime extract, berberine and salts of berberine.

[0060]

[13] A method for promoting placental growth factor production, comprising administering extracts of Phellodendron bark and / or Citrus reticulata peel to a subject.

[0061]

[14] A method for promoting placental growth factor production, comprising administering to a subject one or more of a group consisting of lime extract, berberine and salts of berberine.

[0062]

[15] A method for promoting hair elongation and / or promoting hair thickening, comprising administering a Phellodendron bark extract and / or a dried tangerine peel extract to a subject.

[0063]

[16] A method for promoting hair elongation and / or promoting hair thickening, comprising administering to a subject one or more of a group consisting of lime extract, berberine and salts of berberine.

[0064] [Example]

[0065] The present invention will now be specifically described based on experimental examples, but the invention is not limited thereto. Furthermore, unless otherwise stated, the dosages in the following experimental examples represent mass percentages. Moreover, unless otherwise stated, the culture conditions and other experimental conditions for each test solution in the experimental examples were conducted under the same conditions, except for the addition of Phellodendron amurense extract, Citrus reticulata extract, Citrus aurantium extract, berberine, berberine salts, hydroxycamptothecine, and topotecan.

[0066] [Experimental Example 1: Evaluation of Placental Growth Factor Production]

[0067] Human hair dermal papilla cells (HFDPC) (manufactured by Cell Applications, Inc., product number CA60205a) were used to form 5 × 10 3 Cells were seeded in 96-well plates using a cell / well method and cultured for 24 hours in medium containing 10% FBS (Thermo Fisher, product number 11995-073). Next, the medium was replaced with medium containing 0.1% FBS, including Phellodendron amurense extract and / or Citrus reticulata peel extract, Citrus aurantium extract, or berberine chloride. The Phellodendron amurense extract and Citrus reticulata peel extract were used as... Figures 1-2 The final concentrations shown are prepared by using lime extract and berberine chloride to become... Figure 3 The final concentration was prepared as shown. After culturing for 72 hours, the culture supernatant was recovered, and the amount of PlGF produced in the culture supernatant was measured by ELISA (enzyme-linked immunosorbent assay) using a Human PlGF ELISA Kit (manufactured by R&D Systems, product number DY264). Additionally, in 96-well plates after recovering the culture supernatant, a nuclear staining agent (Hoechst 33342 (manufactured by DOJINDO, product number H342) diluted 1000 times with culture medium was added. After 30 minutes, cell nuclei were counted using an imaging device to obtain the cell number. The amount of PlGF produced per cell was calculated and defined as the PlGF production capacity of the test solution. The PlGF production promotion rate was calculated according to [Equation 1]. Furthermore, those treated in the same way, except for those containing any of the following: Phellodendron bark extract, Citrus reticulata extract, Citrus aurantium extract, or berberine chloride, served as the control group. The results are shown in... Figure 1 .

[0068] [Equation 1]: PlGF production promotion rate (%) = PlGF production capacity of the test solution / PlGF production capacity of the control group × 100

[0069] like Figures 1-2 As shown, the test solution containing Phellodendron bark extract and / or tangerine peel extract had a higher PlGF production promotion rate compared to the control group. Phellodendron bark extract and tangerine peel extract promoted PlGF production. It is believed that the composition containing Phellodendron bark extract and / or tangerine peel extract has a hair elongation effect by promoting PlGF production in hair dermal papilla cells.

[0070] like Figure 3 As shown, the test solution containing lime extract or berberine chloride exhibited a higher rate of PlGF production promotion compared to the control group. Lime extract and berberine chloride promoted PlGF production. It is believed that the composition containing lime extract or berberine chloride has a hair elongation effect by promoting PlGF production in hair dermal papilla cells.

[0071] [Experimental Example 2: Evaluation of Placental Growth Factor Production]

[0072] Using the same method as in Example 1, the production amounts of PlGF with respect to hydroxycamptothecine (CAS registration number: 64439-81-2) and topotecan (CAS registration number: 123948-87-8) were calculated and defined as the PlGF production capacity of the test solution. Hydroxycamptothecine and topotecan were prepared to a final concentration of 10 μM. The PlGF production promotion rate was calculated according to [Equation 1]. Furthermore, those treated in the same manner, except for those containing neither hydroxycamptothecine nor topotecan, served as the control group.

[0073] Hydroxycamptothecin promotes PlGF production by 363%. Since hydroxycamptothecin has been known to promote PlGF production, compositions containing hydroxycamptothecin can serve as placental growth factor production promoters.

[0074] Topotecan promotes PlGF production by 332%. Since topotecan has been proven to promote PlGF production, compositions containing topotecan can serve as placental growth factor production promoters.

[0075] [Experimental Example 3: Evaluation of Hair Growth]

[0076] Hair follicles excised from scalp via puncture biopsy were isolated by immersion in PBS containing an antibacterial agent. The follicles were then placed in 24-well plates. The bulb of the follicle was observed using a digital microscope to confirm the hair cycle was in the anagen phase. Next, the length of the hair shaft was measured and recorded as the initial value. Hair growth medium was added to each well, ensuring complete immersion of the bulb, and the plates were incubated at 37°C with 5% CO2. The following day, the length of the hair shaft was measured using a digital microscope, and the observed elongated follicles were used for further experiments.

[0077] Phellodendron bark extract and / or tangerine peel extract were dissolved in a culture medium and added to the pores containing hair follicles. The Phellodendron bark extract and / or tangerine peel extract were prepared to the final concentrations shown in Tables 1-2. After incubation at 37°C with 5% CO2, the culture medium was recovered, and the hair follicles were photographed using a digital microscope. Subsequently, culture medium was added again for continued incubation. The incubation period from the start of incubation to photographing was defined as 2 days, 4 days, 6 days, and 8 days. Those who underwent the experiment under the same conditions, except for those not containing either Phellodendron bark extract or tangerine peel extract, served as the control group.

[0078] Using CyQUANT TM The LDH Cytotoxicity Assay Kit (Invitrogen) measures LDH in recovered culture media as an indicator of cytotoxicity. In all recovered media, LDH levels were below the detection limit, and no cytotoxicity was observed.

[0079] Hair length was measured during each culture period from self-captured hair follicle images. Hair length was defined as the portion emerging from the epidermis and the hair tip as the endpoint. Using [Equation 2], the change in hair elongation relative to the control group was calculated for the samples containing Phellodendron amurense extract and / or Citrus reticulata extract. The results are shown in Table 1.

[0080] [Equation 2]: Change in hair elongation relative to the control group = (hair length of the test sample after 4 days - hair length of the test sample after 2 days) / (hair length of the control group after 4 days - hair length of the control group after 2 days)

[0081]

[0082] Secondly, using [Equation 3], the elongation of the hair after 8 days of cultivation was calculated relative to the length of the hair after 2 days of cultivation, which was taken as the rate of change of hair elongation after 8 days. The results are shown in Table 2.

[0083] [Equation 3]: The rate of change in hair elongation after 8 days (%) = (Hair length of the test sample after 8 days - Hair length of the test sample after 2 days) / (Hair length of the test sample after 2 days) × 100

[0084]

[0085] As shown in Tables 1 and 2, the test solutions containing Phellodendron amurense extract and / or Citrus reticulata extract showed a greater change in hair elongation compared to the control group, indicating that they promoted hair growth. Compared to the control group, the test solutions containing Phellodendron amurense extract and / or Citrus reticulata extract showed a greater rate of change in hair elongation, thus indicating a higher effect in promoting hair growth.

[0086] [Experimental Example 4: Hair regrowth evaluation (2)]

[0087] Hair follicle organ culture was performed using the same method as in Experimental Example 3. Phellodendron bark extract or dried tangerine peel extract was added to achieve the final concentrations shown in Tables 3-4.

[0088] Hair thickness was measured during each culture period from images of the hair follicles taken from the images. Hair thickness is the measurement of the portion of the hair that has just grown out of the epidermis. Using [Equation 4], the change in hair thickness relative to the control group was calculated for the samples containing Phellodendron amurense extract and Citrus reticulata extract. The results are shown in Table 3.

[0089] [Equation 4]: Change in hair thickness relative to the control group = (hair thickness of the test sample after 4 or 8 days - hair thickness of the test sample after 1 day) / (hair thickness of the control group after 4 or 8 days - hair thickness of the control group after 1 day)

[0090]

[0091] Secondly, using [Equation 5], the change in hair thickness after 2 or 4 days of cultivation relative to the hair thickness after 1 day of cultivation was calculated as the rate of change in hair thickness after 2 or 4 days. The results are shown in Table 4.

[0092] [Equation 5]: Rate of change in hair thickness (%) = (Hair thickness of the test sample after 2 or 4 days - Hair thickness of the test sample after 1 day) / (Hair thickness of the test sample after 1 day) × 100

[0093]

[0094] As shown in Tables 3 and 4, the test solutions containing Phellodendron amurense extract and Citrus reticulata extract showed a larger change in hair thickness compared to the control group, suggesting a promotion of hair growth. Compared to the control group, the test solutions containing both Phellodendron amurense extract and Citrus reticulata extract exhibited a larger rate of change in hair thickness, thus indicating a higher effect in promoting hair growth. Surprisingly, Phellodendron amurense extract and Citrus reticulata extract showed a hair growth effect that increased hair diameter. In the photographed hair follicle images, the conical shape of the hair follicles under the epidermis suggests that the hairs, proliferating and differentiating from the hair matrix, are pushed outwards from the epidermis, resulting in a larger hair shaft diameter and thicker hair at the measured location.

[0095] It is believed that extracts of Phellodendron bark and tangerine peel promote the secretion of PlGF from dermal papilla cells and promote the proliferation or differentiation of hair matrix cells, thereby promoting the increase of hair elongation or thickness.

Claims

1. A hair and / or scalp composition comprising Phellodendron bark extract and Citrus reticulata peel extract.

2. The hair and / or scalp composition according to claim 1, which promotes the production of placental growth factor by dermal papilla cells.

3. A placental growth factor production promoter, comprising one or more ingredients selected from the group consisting of Phellodendron bark extract, Citrus reticulata extract, Citrus aurantium extract, berberine, and salts of berberine.

4. A hair and / or scalp composition for promoting placental growth factor production, comprising one or more ingredients selected from the group consisting of lime extract, berberine, and salts of berberine.

Images