A simple two-step in vitro method for evaluating the nutritional value of horse energy feeds

CN123029169APending Publication Date: 2026-11-03TARIM UNIV
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Patent Information

Application Number
CN202410974824.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-07-19
Publication Date
2026-11-03

AI Technical Summary

Technical Problem

Existing methods for evaluating the nutritional content of donkey energy feed require slaughtering the animal or performing fistula surgery, which is costly and causes stress damage to the animal. The in vivo nylon bag method is accurate but also carries the risk of animal stress.

Method used

The method employs a two-step in vitro approach, using nylon bags to simulate the digestion of donkey feces by gastric and small intestinal enzymes, combined with artificial hindgut fermentation, and inoculation via rectal feces to avoid fistula surgery. The use of simulated liquids and microbial fermentation simplifies the operation process.

Benefits of technology

It enables low-cost, reliable nutritional evaluation of donkey energy feed without the need for fistula surgery. The operation is simple, causes no harm to animals, and the results are reliable and suitable for long-term application.

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Abstract

The application discloses a simple two-step method for evaluating the nutritional value of donkey energy feed in vitro, relates to the field of donkey energy feed nutritional value evaluation, and solves the problems that the existing evaluation methods usually need to slaughter animals or perform fistula surgery, are high in cost, inconvenient to maintain, and accurate in the in-vivo nylon bag method but have stress damage to animals, and proposes the following scheme, which comprises the following steps of S100, preliminary preparation: accurately weighing 1.50+ / -0.10 g of various feed samples and loading the samples into nylon bags for sealing and standby; S200, in-vitro enzyme test stage: preparing gastric juice simulation liquid and small intestinal juice simulation liquid and performing enzyme digestion; S300, artificial hindgut liquid preparation; and S400, in-vitro fermentation test stage. The method is more time-saving and labor-saving, has little stress to animals, is low in cost, can be performed at any time, and is not limited by fistula animals and digestion and metabolism devices.
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