A pet anti-calculus gel and method of making same

Abstract

The application belongs to the technical field of pet products, and specifically discloses a pet anti-calculus gel and a preparation method thereof. e - polylysine hydrochloride, 0.05-0.2% bacterial cellulose dispersion, 0.08-0.2% biological enzyme, 0.2-0.6% antioxidant, 0.2-0.6% repairing agent, 0.5-0.8% thickening agent, 0.6-0.9% fucoidan, 0.8-1.2% plant fermentation extract, 1-2% erythritol, 1-2% sorbitol, 10-15% glycerol, and the balance of water. The pet anti-calculus gel has a good inhibitory effect on key pathogenic bacteria of dental plaque and calculus, and has a good calculus removal effect.

Description

Technical Field

[0001] This invention relates to the field of pet product technology, specifically to a pet anti-tartar gel and its preparation method. Background Technology

[0002] Oral diseases are the most common chronic health problems among pet dogs and cats, with plaque buildup, tartar mineralization, secondary halitosis, and mild gingivitis being the most prevalent symptoms. Tartar is formed by the long-term deposition and mineralization of metabolic products of oral pathogens, food debris, and calcium and phosphorus minerals in saliva. Once hardened, it is difficult to remove with regular brushing, continuously irritating the gums, causing redness, swelling, and recession. In severe cases, it can lead to periodontitis, oral ulcers, and even induce systemic metabolic problems, seriously affecting the pet's quality of life.

[0003] Currently available pet anti-tartar gels and oral care products still have many technical shortcomings. Most products rely on chemical antibacterial agents, strong chelating agents, or acidic softening ingredients to achieve temporary teeth cleaning effects. Long-term use can easily cause micro-damage to tooth enamel and irritation to the oral mucosa. In addition, some quaternary ammonium salt antibacterial ingredients can easily cause tooth discoloration and a bitter taste, resulting in low acceptance by pets.

[0004] Therefore, this application is submitted. Summary of the Invention

[0005] This invention provides a pet anti-tartar gel and its preparation method. The pet anti-tartar gel described in this application has a good inhibitory effect on key pathogenic bacteria of dental plaque and tartar, and has a good tartar removal effect.

[0006] The present invention solves its technical problem by adopting the following technical solution: A pet anti-tartar gel comprising the following components by weight percentage: 0.05~0.2% steviol glycosides, 0.05~0.08%... e - Polylysine hydrochloride, 0.05~0.2% bacterial cellulose dispersion, 0.08~0.2% bio-enzyme, 0.2~0.6% antioxidant, 0.2~0.6% repair agent, 0.5~0.8% thickener, 0.6~0.9% brown algae polysaccharide sulfate, 0.8~1.2% plant fermentation extract, 1~2% erythritol, 1~2% sorbitol, 10~15% glycerin, balance water.

[0007] As an embodiment of this application, the thickener is at least one of carrageenan, xanthan gum, carboxymethyl cellulose, and microcrystalline cellulose.

[0008] As an embodiment of this application, the bio-enzyme is at least one of glucanase, alkaline protease, and lysozyme.

[0009] As an embodiment of this application, the antioxidant is...α- Tocopherol acetate.

[0010] As an embodiment of this application, the repair agent is dipotassium glycyrrhizate.

[0011] As an embodiment of this application, the method for preparing the plant fermentation extract is as follows: (1) Mix Rhodiola rosea powder and lotus leaf powder evenly to obtain mixed powder. Add the mixed powder to water, extract by ultrasonication, filter, and obtain filtrate. (2) Mix the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate and dipotassium hydrogen phosphate evenly, sterilize, and obtain the fermentation substrate; (3) Inoculate Lactobacillus plantarum and Lactobacillus rhamnosus into the fermentation substrate, ferment, filter, sterilize, and obtain plant fermentation extract.

[0012] As an embodiment of this application, the mass ratio of Rhodiola rosea powder to lotus leaf powder is 1:(0.8~1.5).

[0013] As an embodiment of this application, the solid-liquid ratio of the mixed powder and water is 1g:(5~20)mL.

[0014] As an embodiment of this application, the ultrasonic extraction power is 200~500W, the time is 1~8h, and the temperature is 50~85°C.

[0015] As an embodiment of this application, the mass ratio of the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate, and dipotassium hydrogen phosphate is 100:(2~3):(1.2~2):(4~5):(0.05~0.2):(0.02~0.08).

[0016] As an embodiment of this application, the inoculum amount of Lactobacillus plantarum is 0.8~1.2wt%.

[0017] As an embodiment of this application, the inoculation amount of Lactobacillus rhamnosus is 0.5~0.8wt%.

[0018] As an embodiment of this application, the fermentation temperature is 34~38°C and the fermentation time is 36~72h.

[0019] This application also provides a method for preparing a pet anti-tartar gel, comprising the following steps: Bacterial cellulose dispersion, thickener, e - Mix polylysine hydrochloride, erythritol, sorbitol, glycerin and water evenly, then add steviol glycosides, brown algae polysaccharide sulfate, biological enzymes, antioxidants, repair agents and plant fermentation extracts, stir evenly to obtain pet anti-tartar gel.

[0020] The beneficial effects of this invention: The pet anti-tartar gel described in this application, through... e - Polylysine hydrochloride, bacterial cellulose dispersion, biological enzymes, repair agents, brown algae polysaccharide sulfate, and plant fermentation extracts achieve stable adhesion to teeth. Through multiple functions such as mineral inhibition, antibacterial, caries prevention, and tooth surface repair, it has a good inhibitory effect on key pathogens of dental plaque and tartar, and has a good tartar removal effect. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.

[0023] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0024] In this invention, there are no particular limitations on the specific dispersion and stirring methods.

[0025] Unless otherwise specified, all reagents or instruments used in this invention are commercially available conventional products. Unless otherwise specified, the raw materials used in each comparative example and the parallel experiments of each embodiment are the same commercially available products.

[0026] The lysozyme used in the examples and comparative examples was derived from Xiasheng (Beijing) Biotechnology, SDG2447.

[0027] Among them, the examples and comparative examples e - Polylysine hydrochloride is sourced from Zhejiang Xinyinxiang Biotechnology.

[0028] The brown algae polysaccharide sulfates used in the examples and comparative examples were sourced from Jinan Shenghe Chemical Co., Ltd.

[0029] The *Lactobacillus plantarum* used in the examples and comparative examples was obtained from the Guangdong Provincial Microbial Culture Collection Center, number 1.2685.

[0030] The Lactobacillus rhamnosus used in the examples and comparative examples was obtained from the Guangdong Provincial Microbial Culture Collection Center, number 1.2223.

[0031] The bacterial cellulose dispersions used in the examples and comparative examples were derived from Tianlu Nano, TL-008.

[0032] This application provides a pet anti-tartar gel comprising the following components by weight percentage: 0.05~0.2% steviol glycosides, 0.05~0.08%... e - Polylysine hydrochloride, 0.05~0.2% bacterial cellulose dispersion, 0.08~0.2% bio-enzyme, 0.2~0.6% antioxidant, 0.2~0.6% repair agent, 0.5~0.8% thickener, 0.6~0.9% brown algae polysaccharide sulfate, 0.8~1.2% plant fermentation extract, 1~2% erythritol, 1~2% sorbitol, 10~15% glycerin, balance water.

[0033] The pet anti-tartar gel described in this application, through... e - Polylysine hydrochloride, bacterial cellulose dispersion, biological enzymes, repair agents, brown algae polysaccharide sulfate, and plant fermentation extracts achieve stable adhesion to teeth. Through multiple functions such as mineral inhibition, antibacterial, caries prevention, and tooth surface repair, it has a good inhibitory effect on key pathogens of dental plaque and tartar, and has a good tartar removal effect.

[0034] Among them, bio-enzymes can effectively hydrolyze glucan and protein matrix in dental plaque, destroy the oral biofilm skeleton, loosen the organic attachment layer of soft plaque and early tartar on the tooth surface, and provide a basis for the subsequent penetration of active ingredients. e Polylysine hydrochloride can disrupt the cell membrane structure of periodontal pathogens, inhibit the proliferation of harmful bacteria, and reduce oral odor. Bacterial cellulose dispersions possess excellent nano-network dispersion and film-forming properties, stabilizing functional microparticles within the system, preventing the sedimentation and stratification of active components, and simultaneously assisting the gel in forming a uniform wetting film on the tooth surface, delaying saliva rinsing and improving the overall duration of action.

[0035] Among them, after Rhodiola rosea and lotus leaves are fermented with lactic acid bacteria, the macromolecular bound flavonoids and polyphenols in the raw materials are biotransformed to generate a large number of small molecule free flavonoids, phenolic acids and lactic acid bacteria metabolites. The resulting plant fermentation extract can efficiently penetrate into the interior of dental plaque, exert the effects of chelating calcium ions, inhibiting tooth mineralization deposition, anti-inflammatory and antibacterial effects, and blocking the formation of tartar precursors. At the same time, the fermentation products have significantly improved bioavailability, reducing the defects of ordinary plant extracts such as easy precipitation and poor stability. Fucoidan sulfate is a negatively charged sulfated functional polysaccharide. On the one hand, it can encapsulate and fix small molecule flavonoids and polyphenols in plant fermentation extracts through hydrogen bonding and steric hindrance, significantly improving the dispersion uniformity and storage stability of plant fermentation extracts in aqueous gel systems and preventing the aggregation and oxidation of active ingredients. On the other hand, fucoidan sulfate can firmly bind to the enamel surface through electrostatic adsorption, forming a slow-release hydrophilic film on the tooth surface, anchoring the fermented flavonoid active components to the tooth and gum surface for a long time, greatly resisting saliva erosion, and solving the problems of short residence time, rapid onset of action and rapid deterioration of ordinary plant active substances. Through the synergistic effect of fucoidan sulfate and plant fermentation extracts, the antibacterial effect and the tartar removal effect are effectively improved.

[0036] The stevioside content is 0.05-0.2% by mass, for example, it can be 0.05%, 0.06%, 0.08%, 0.1%, 0.12%, 0.15%, 0.18%, 0.2% or any two of these values.

[0037] Among them, the aforementioned e - The mass percentage of polylysine hydrochloric acid is 0.05~0.08%, for example, it can be 0.05%, 0.06%, 0.07%, 0.08% or any two of these values.

[0038] The bacterial cellulose dispersion has a mass percentage content of 0.05~0.2%, for example, it can be 0.05%, 0.06%, 0.08%, 0.1%, 0.12%, 0.15%, 0.18%, 0.2% or any two of these values.

[0039] The bioenzyme has a mass percentage content of 0.08-0.2%, for example, it can be 0.08%, 0.1%, 0.12%, 0.15%, 0.18%, 0.2%, or any two of these values.

[0040] The antioxidant has a mass percentage content of 0.2-0.6%, for example, it can be 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6% or any two of these values.

[0041] The repair agent has a mass percentage content of 0.2-0.6%, for example, it can be 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6% or any two of these values.

[0042] The thickener has a mass percentage content of 0.5-0.8%, for example, it can be 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, or any two of these values.

[0043] The mass percentage of the brown algae polysaccharide sulfate ester is 0.6-0.9%, for example, it can be 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.9% or any two of these values.

[0044] The plant fermentation extract has a mass percentage content of 0.8-1.2%, for example, it can be 0.8%, 0.85%, 0.9%, 0.95%, 1%, 1.05%, 1.1%, 1.2% or any two of these values.

[0045] The erythritol content is 1-2% by mass, for example, it can be 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2% or any two of these values.

[0046] The sorbitol content is 1-2% by mass, for example, it can be 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2% or any two of these values.

[0047] The glycerol content by mass is 10-15%, for example, it can be 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%, 13.5%, 14%, 14.5%, 15% or any two of these values.

[0048] In some embodiments, the thickener is at least one of carrageenan, xanthan gum, carboxymethyl cellulose, and microcrystalline cellulose.

[0049] In some embodiments, the bioenzyme is at least one of glucanase, alkaline protease, and lysozyme.

[0050] In some embodiments, the antioxidant is α-Tocopherol acetate.

[0051] In some embodiments, the repair agent is dipotassium glycyrrhizate.

[0052] In some embodiments, the method for preparing the plant fermentation extract is as follows: (1) Mix Rhodiola rosea powder and lotus leaf powder evenly to obtain mixed powder. Add the mixed powder to water, extract by ultrasonication, filter, and obtain filtrate. (2) Mix the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate and dipotassium hydrogen phosphate evenly, sterilize, and obtain the fermentation substrate; (3) Inoculate Lactobacillus plantarum and Lactobacillus rhamnosus into the fermentation substrate, ferment, filter, sterilize, and obtain plant fermentation extract.

[0053] In some embodiments, the mass ratio of Rhodiola rosea powder to lotus leaf powder is 1:(0.8~1.5).

[0054] In some embodiments, the solid-liquid ratio of the mixed powder to water is 1 g: (5~20) mL.

[0055] In some embodiments, the ultrasonic extraction power is 200~500W, the time is 1~8h, and the temperature is 50~85°C.

[0056] In some embodiments, the mass ratio of the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate, and dipotassium hydrogen phosphate is 100:(2~3):(1.2~2):(4~5):(0.05~0.2):(0.02~0.08).

[0057] In some embodiments, the inoculum amount of *Lactobacillus plantarum* is 0.8 to 1.2 wt%.

[0058] In some embodiments, the inoculum amount of Lactobacillus rhamnosus is 0.5 to 0.8 wt%.

[0059] In some embodiments, the fermentation temperature is 34-38°C and the fermentation time is 36-72 hours.

[0060] In some embodiments, the particle size of the Rhodiola rosea powder is 20-500 mesh.

[0061] In some embodiments, the lotus leaf powder has a particle size of 20-500 mesh.

[0062] In some embodiments, the solid content of the bacterial cellulose dispersion is 0.5-1%.

[0063] One embodiment of this application provides a method for preparing a pet anti-tartar gel, comprising the following steps: Bacterial cellulose dispersion, thickener, e - Mix polylysine hydrochloride, erythritol, sorbitol, glycerin and water evenly, then add steviol glycosides, brown algae polysaccharide sulfate, biological enzymes, antioxidants, repair agents and plant fermentation extracts, stir evenly to obtain pet anti-tartar gel.

[0064] The present application is further illustrated below with specific embodiments.

[0065] Example 1

[0066] A pet anti-tartar gel comprising the following components by weight percentage: 0.12% steviol glycosides, 0.06%... e - Polylysine hydrochloride, 0.15% bacterial cellulose dispersion, 0.16% lysozyme, 0.4% α- Tocopheryl acetate, 0.5% dipotassium glycyrrhizate, 0.6% xanthan gum, 0.8% brown algae polysaccharide sulfate, 1% plant fermentation extract, 1.5% erythritol, 1.6% sorbitol, 12% glycerin, balance water.

[0067] The preparation method of the plant fermentation extract is as follows: (1) Wash and dry Rhodiola rosea and lotus leaves, then grind them to 50 mesh to obtain Rhodiola rosea powder and lotus leaf powder respectively.

[0068] Rhodiola rosea powder and lotus leaf powder were mixed evenly at a mass ratio of 1:1 to obtain a mixed powder. The mixed powder was added to water and extracted at 400W ultrasonication and 70°C for 5 hours. The mixture was then filtered to obtain a filtrate. The solid-liquid ratio of the mixed powder to water was 1g:8mL. (2) Mix the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate and dipotassium hydrogen phosphate in a mass ratio of 100:2.5:1.8:4.5:0.15:0.06 and sterilize at 90°C for 30 min to obtain the fermentation substrate; (3) Add the fermentation substrate to the fermentation tank, then inoculate with 1 wt% Lactobacillus plantarum and 0.6 wt% Lactobacillus rhamnosus, ferment at 36°C for 48 h, filter, sterilize at 90°C for 30 min, and obtain plant fermentation extract.

[0069] The preparation method of the pet anti-tartar gel includes the following steps: Bacterial cellulose dispersion, xanthan gum, e Polylysine hydrochloride, erythritol, sorbitol, glycerol, and water are mixed thoroughly at 65°C, then cooled to 40°C before adding steviol glycosides, fucoidan sulfate, and lysozyme. α-Tocopheryl acetate, dipotassium glycyrrhizate, and plant fermentation extract were mixed evenly to obtain a pet anti-tartar gel.

[0070] Example 2 A pet anti-tartar gel comprising the following components by weight percentage: 0.2% steviol glycosides, 0.05%... e - Polylysine hydrochloride, 0.05% bacterial cellulose dispersion, 0.2% lysozyme, 0.2% α- Tocopheryl acetate, 0.6% dipotassium glycyrrhizate, 0.5% xanthan gum, 0.9% brown algae polysaccharide sulfate, 0.8% plant fermentation extract, 2% erythritol, 1% sorbitol, 15% glycerin, balance water.

[0071] The preparation method of the plant fermentation extract is as follows: (1) Wash and dry Rhodiola rosea and lotus leaves, then grind them to 50 mesh to obtain Rhodiola rosea powder and lotus leaf powder respectively.

[0072] Rhodiola rosea powder and lotus leaf powder were mixed evenly at a mass ratio of 1:0.8 to obtain a mixed powder. The mixed powder was added to water and extracted at 400W ultrasonication and 70°C for 5 hours. The mixture was then filtered to obtain a filtrate. The solid-liquid ratio of the mixed powder to water was 1g:8mL. (2) Mix the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate and dipotassium hydrogen phosphate in a mass ratio of 100:2:2:4:0.05:0.08 and sterilize at 90°C for 30 min to obtain the fermentation substrate; (3) Add the fermentation substrate to the fermentation tank, then inoculate with 1 wt% Lactobacillus plantarum and 0.6 wt% Lactobacillus rhamnosus, ferment at 36°C for 48 h, filter, sterilize at 90°C for 30 min, and obtain plant fermentation extract.

[0073] The preparation method of the pet anti-tartar gel includes the following steps: Bacterial cellulose dispersion, xanthan gum, e Polylysine hydrochloride, erythritol, sorbitol, glycerol, and water are mixed thoroughly at 65°C, then cooled to 40°C before adding steviol glycosides, fucoidan sulfate, and lysozyme. α- Tocopheryl acetate, dipotassium glycyrrhizate, and plant fermentation extract were mixed evenly to obtain a pet anti-tartar gel.

[0074] Example 3 A pet anti-tartar gel comprising the following components by weight percentage: 0.05% steviol glycosides, 0.08%... e - Polylysine hydrochloride, 0.2% bacterial cellulose dispersion, 0.08% lysozyme, 0.6% α-Tocopheryl acetate, 0.2% dipotassium glycyrrhizate, 0.8% xanthan gum, 0.6% brown algae polysaccharide sulfate, 1.2% plant fermentation extract, 1% erythritol, 2% sorbitol, 10% glycerin, balance water.

[0075] The preparation method of the plant fermentation extract is as follows: (1) Wash and dry Rhodiola rosea and lotus leaves, then grind them to 50 mesh to obtain Rhodiola rosea powder and lotus leaf powder respectively.

[0076] Rhodiola rosea powder and lotus leaf powder were mixed evenly at a mass ratio of 1:1.5 to obtain a mixed powder. The mixed powder was added to water and extracted at 400W ultrasonication and 70°C for 5 hours. The mixture was then filtered to obtain a filtrate. The solid-liquid ratio of the mixed powder to water was 1g:8mL. (2) Mix the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate and dipotassium hydrogen phosphate in a mass ratio of 100:3:1.2:5:0.2:0.02 and sterilize at 90°C for 30 min to obtain the fermentation substrate; (3) Add the fermentation substrate to the fermentation tank, then inoculate with 1 wt% Lactobacillus plantarum and 0.6 wt% Lactobacillus rhamnosus, ferment at 36°C for 48 h, filter, sterilize at 90°C for 30 min, and obtain plant fermentation extract.

[0077] The preparation method of the pet anti-tartar gel includes the following steps: Bacterial cellulose dispersion, xanthan gum, e Polylysine hydrochloride, erythritol, sorbitol, glycerol, and water are mixed thoroughly at 65°C, then cooled to 40°C before adding steviol glycosides, fucoidan sulfate, and lysozyme. α- Tocopheryl acetate, dipotassium glycyrrhizate, and plant fermentation extract were mixed evenly to obtain a pet anti-tartar gel.

[0078] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 uses an equal amount of brown algae polysaccharide sulfate to replace the plant fermentation extract, while everything else is the same.

[0079] A pet anti-tartar gel comprising the following components by weight percentage: 0.12% steviol glycosides, 0.06%... e - Polylysine hydrochloride, 0.15% bacterial cellulose dispersion, 0.16% lysozyme, 0.4% α- Tocopheryl acetate, 0.5% dipotassium glycyrrhizate, 0.6% xanthan gum, 1.8% fucoxanthin sulfate, 1.5% erythritol, 1.6% sorbitol, 12% glycerol, balance water.

[0080] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 uses an equal amount of plant fermentation extract to replace brown algae polysaccharide sulfate, while everything else is the same.

[0081] A pet anti-tartar gel comprising the following components by weight percentage: 0.12% steviol glycosides, 0.06%... e - Polylysine hydrochloride, 0.15% bacterial cellulose dispersion, 0.16% lysozyme, 0.4% α- Tocopheryl acetate, 0.5% dipotassium glycyrrhizate, 0.6% xanthan gum, 1.8% plant fermentation extract, 1.5% erythritol, 1.6% sorbitol, 12% glycerin, balance water.

[0082] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that the preparation method of the plant fermentation extract is different, but everything else is the same.

[0083] A pet anti-tartar gel comprising the following components by weight percentage: 0.12% steviol glycosides, 0.06%... e - Polylysine hydrochloride, 0.15% bacterial cellulose dispersion, 0.16% lysozyme, 0.4% α- Tocopheryl acetate, 0.5% dipotassium glycyrrhizate, 0.6% xanthan gum, 0.8% brown algae polysaccharide sulfate, 1% plant fermentation extract, 1.5% erythritol, 1.6% sorbitol, 12% glycerin, balance water.

[0084] The preparation method of the plant fermentation extract is as follows: (1) Wash and dry the Rhodiola rosea, then grind it to 50 mesh to obtain Rhodiola rosea powder.

[0085] Rhodiola rosea powder was added to water and extracted at 400W ultrasonication and 70°C for 5 hours. The mixture was then filtered to obtain the filtrate. The solid-liquid ratio of Rhodiola rosea powder to water was 1g:8mL. (2) Mix the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate and dipotassium hydrogen phosphate in a mass ratio of 100:2.5:1.8:4.5:0.15:0.06 and sterilize at 90°C for 30 min to obtain the fermentation substrate; (3) Add the fermentation substrate to the fermentation tank, then inoculate with 1 wt% Lactobacillus plantarum and 0.6 wt% Lactobacillus rhamnosus, ferment at 36°C for 48 h, filter, sterilize at 90°C for 30 min, and obtain plant fermentation extract.

[0086] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that the preparation method of the plant fermentation extract is different, but everything else is the same.

[0087] A pet anti-tartar gel comprising the following components by weight percentage: 0.12% steviol glycosides, 0.06%... e - Polylysine hydrochloride, 0.15% bacterial cellulose dispersion, 0.16% lysozyme, 0.4% α- Tocopheryl acetate, 0.5% dipotassium glycyrrhizate, 0.6% xanthan gum, 0.8% brown algae polysaccharide sulfate, 1% plant fermentation extract, 1.5% erythritol, 1.6% sorbitol, 12% glycerin, balance water.

[0088] The preparation method of the plant fermentation extract is as follows: (1) Wash the lotus leaves, dry them, and then grind them to 50 mesh to obtain lotus leaf powder.

[0089] Lotus leaf powder was added to water and extracted at 400W ultrasonication and 70°C for 5 hours. The mixture was then filtered to obtain the filtrate. The solid-liquid ratio of lotus leaf powder to water was 1g:8mL. (2) Mix the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate and dipotassium hydrogen phosphate in a mass ratio of 100:2.5:1.8:4.5:0.15:0.06 and sterilize at 90°C for 30 min to obtain the fermentation substrate; (3) Add the fermentation substrate to the fermentation tank, then inoculate with 1 wt% Lactobacillus plantarum and 0.6 wt% Lactobacillus rhamnosus, ferment at 36°C for 48 h, filter, sterilize at 90°C for 30 min, and obtain plant fermentation extract.

[0090] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that an equal amount of plant extract was used to replace the plant fermentation extract, while everything else is the same.

[0091] A pet anti-tartar gel comprising the following components by weight percentage: 0.12% steviol glycosides, 0.06%... e - Polylysine hydrochloride, 0.15% bacterial cellulose dispersion, 0.16% lysozyme, 0.4% α- Tocopheryl acetate, 0.5% dipotassium glycyrrhizate, 0.6% xanthan gum, 0.8% brown algae polysaccharide sulfate, 1% plant extract, 1.5% erythritol, 1.6% sorbitol, 12% glycerin, balance water.

[0092] The method for preparing the plant extract is as follows: (1) Wash and dry Rhodiola rosea and lotus leaves, then grind them to 50 mesh to obtain Rhodiola rosea powder and lotus leaf powder respectively.

[0093] Rhodiola rosea powder and lotus leaf powder were mixed evenly at a mass ratio of 1:1 to obtain a mixed powder. The mixed powder was added to water and extracted at 400W ultrasonication and 70°C for 5 hours. After filtration, the plant extract was obtained. The solid-liquid ratio of the mixed powder to water was 1g:8mL.

[0094] Test case 1. Stability Testing Weigh 20g of each of the pet anti-tartar gels from the examples and comparative cases into transparent bottles, and then separately... - The samples were placed at constant temperatures of 8°C, 25°C, and 45°C for 90 days. After returning to room temperature, their stability was observed. The criteria for judging stability were that the sample did not show any signs of layering or oil seepage after returning to room temperature, and the transparency did not change. Otherwise, the sample was considered to be abnormal.

[0095] Table 1. Stability Test Results

[0096] As can be seen from Table 1, the pet anti-tartar gel described in this application has good stability.

[0097] 2. The antibacterial effect was tested according to the inhibition zone method in QB / T2738-2023. The test bacteria were Porphyromonas gingivalis and Fusobacterium nucleatum.

[0098] Table 2 Antibacterial Effect Table

[0099] As can be seen from Table 2, the pet anti-tartar gel described in this application has a good inhibitory effect on the key pathogens causing dental plaque and tartar.

[0100] 3. Efficacy Test: Thirty dogs with dental calculus were selected and randomly divided into 6 groups of 5 each. Prior to the experiment, the dogs underwent routine immunization and deworming treatment. The test pets should meet the inclusion and exclusion criteria to eliminate any factors that might interfere with the experimental objectives.

[0101] Inclusion criteria: Adult dogs in good overall health; check teeth and gums, with at least 20 natural teeth in the mouth; no antibiotics or antimicrobial drugs used in the month prior to the start of testing; signed informed consent form.

[0102] Exclusion criteria: Pets participating in other clinical studies; pets using oral hygiene products containing phenols or mint flavorings, such as mouthwash, on the day of the study; pets with a history of allergy to the test sample or its ingredients; pets with serious illnesses; pets unable to comply with the test requirements due to physical reasons; pets unable to meet the fasting or abstaining from water requirements within the evaluation period due to medical reasons; and pets in special physiological periods (estrus, pregnancy, lactation, menstruation).

[0103] The gels prepared in Example 1 and Comparative Examples 1-5 were applied to the tooth surfaces of experimental dogs with tartar. The length and width of the tartar in each dog were measured on day 0. A second measurement of tartar length and width was performed after 14 days of continuous application. Three teeth from each dog were used in the testing process, and the teeth were used for both the initial and subsequent measurements. The average value for each group was calculated.

[0104] Calculate the calculus length reduction rate = 100% (Average length of calculus in anterior teeth - Average length of calculus in posterior teeth) / Average length of calculus in anterior teeth.

[0105] Calculate the rate of decrease in tartar width = 100% (Average width of calculus in anterior teeth - Average width of calculus in posterior teeth) / Average width of calculus in anterior teeth.

[0106] Table 3 Results of Anti-calculus Test

[0107] As can be seen from Table 3, the pet anti-tartar gel described in this application has excellent tartar removal effect.

[0108] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A pet anti-tartar gel, characterized in that, It includes the following components by weight percentage: 0.05~0.2% steviol glycosides, 0.05~0.08%... ε - Polylysine hydrochloride, 0.05~0.2% bacterial cellulose dispersion, 0.08~0.2% bio-enzyme, 0.2~0.6% antioxidant, 0.2~0.6% repair agent, 0.5~0.8% thickener, 0.6~0.9% brown algae polysaccharide sulfate, 0.8~1.2% plant fermentation extract, 1~2% erythritol, 1~2% sorbitol, 10~15% glycerin, balance water.

2. The pet anti-tartar gel according to claim 1, characterized in that, The thickener is at least one of carrageenan, xanthan gum, carboxymethyl cellulose, and microcrystalline cellulose.

3. The pet anti-tartar gel according to claim 1, characterized in that, The bioenzyme is at least one of glucanase, alkaline protease, and lysozyme.

4. The pet anti-tartar gel according to claim 1, characterized in that, The antioxidant is α- Tocopherol acetate; The repair agent is dipotassium glycyrrhizate.

5. The pet anti-tartar gel according to claim 1, characterized in that, The preparation method of the plant fermentation extract is as follows: (1) Mix Rhodiola rosea powder and lotus leaf powder evenly to obtain mixed powder. Add the mixed powder to water, extract by ultrasonication, filter, and obtain filtrate. (2) Mix the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate and dipotassium hydrogen phosphate evenly, sterilize, and obtain the fermentation substrate; (3) Inoculate Lactobacillus plantarum and Lactobacillus rhamnosus into the fermentation substrate, ferment, filter, sterilize, and obtain plant fermentation extract.

6. The pet anti-tartar gel according to claim 1, characterized in that, The mass ratio of Rhodiola rosea powder to lotus leaf powder is 1:(0.8~1.5).

7. The pet anti-tartar gel according to claim 1, characterized in that, The solid-liquid ratio of the mixed powder and water is 1g:(5~20)mL; The ultrasonic extraction was performed at a power of 200-500W, a time of 1-8 hours, and a temperature of 50-85°C.

8. The pet anti-tartar gel according to claim 1, characterized in that, The mass ratio of the filtrate, peptone, yeast powder, glucose, magnesium sulfate heptahydrate, and dipotassium hydrogen phosphate is 100:(2~3):(1.2~2):(4~5):(0.05~0.2):(0.02~0.08).

9. The pet anti-tartar gel according to claim 1, characterized in that, The inoculum size of *Lactobacillus plantarum* is 0.8–1.2 wt%. The inoculum size of *Lactobacillus rhamnosus* is 0.5-0.8 wt%. The fermentation temperature is 34~38°C, and the fermentation time is 36~72h.

10. The method for preparing the pet anti-tartar gel according to any one of claims 1 to 9, characterized in that, Includes the following steps: Bacterial cellulose dispersion, thickener, ε- Polylysine hydrochloride, erythritol, sorbitol, glycerin and water are mixed evenly, then steviol glycosides, brown algae polysaccharide sulfate, biological enzymes, antioxidants, repair agents and plant fermentation extracts are added and stirred evenly to obtain pet anti-tartar gel.

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