A soothing repair composition for photodamage and thermal damage and a method of making the same

Abstract

This invention discloses a soothing and repairing composition targeting photodamage and temperature damage, and its preparation method, belonging to the technical field of daily cosmetics. The composition is a complex of Lactobacillus / algae extract fermentation products, Dunaliella salina extract, Buddleja officinalis extract, lactoferrin, and glyceryl glucoside. This composition exhibits synergistic multi-target effects of antioxidation, anti-inflammation, and barrier repair, significantly improving the survival rate of cells damaged by photodamage and heat, inhibiting excessive IL-6 secretion, effectively improving skin redness, barrier damage, and other discomforts. It is also gentle and safe, suitable for preparing various soothing and repairing cosmetics, and has broad application prospects.

Description

Technical Field

[0001] This invention belongs to the technical field of daily cosmetics, specifically relating to a soothing and repairing composition for photodamage and temperature damage, and its preparation method. Background Technology

[0002] As the largest organ in the human body, the skin is the first line of defense against external environmental stimuli. In daily life, the skin is frequently exposed to various environmental factors such as ultraviolet radiation and extreme temperature changes, which can easily lead to photodamage and temperature damage, manifesting as symptoms such as redness, burning, stinging, dryness, peeling, and impaired barrier function.

[0003] Ultraviolet (UV) radiation is the main cause of photodamage to the skin. Medium-wave ultraviolet (UVB) radiation can directly act on epidermal keratinocytes, inducing the production of reactive oxygen species (ROS), triggering oxidative stress, and leading to the massive release of inflammatory factors such as IL-6 and TNF-α. This results in acute damage such as cell apoptosis, skin erythema, and edema. Long-term, repeated photodamage can also accelerate skin aging, leading to problems such as pigmentation and increased wrinkles.

[0004] Temperature damage is also a significant contributing factor to skin barrier impairment. High temperatures and alternating hot and cold stimuli can disrupt the structure of the stratum corneum, leading to the loss of lipid components such as ceramides and a decline in skin barrier function. Simultaneously, high temperatures can induce abnormal expression of heat shock proteins, triggering an inflammatory cascade and exacerbating skin sensitivity. Low temperatures in winter slow blood circulation and reduce the moisture content of the stratum corneum, further aggravating dryness and barrier damage.

[0005] Most commercially available soothing and repairing products use single active ingredients, such as panthenol, allantoin, and dipotassium glycyrrhizate. While these offer some soothing effects, they often target only a single area and cannot simultaneously address the multiple skin problems caused by photodamage and temperature damage. Furthermore, some products add high concentrations of irritating ingredients in pursuit of immediate soothing effects, which may actually worsen skin conditions and be unsuitable for those with sensitive skin.

[0006] Therefore, developing a soothing and repairing composition that can simultaneously target both light and heat damage, has synergistic effects on multiple targets, and is gentle and safe has significant market value and application prospects. Summary of the Invention

[0007] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a soothing and repairing composition for photodamage and temperature damage, as well as a method for its preparation. This composition, through the scientific compounding of multiple active ingredients, exerts a synergistic effect of antioxidation, anti-inflammation, and barrier repair, effectively alleviating skin damage caused by ultraviolet radiation and temperature changes, and improving discomfort symptoms such as redness, burning, and dryness.

[0008] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides a soothing and repairing composition for photodamage and temperature damage, the composition comprising the following ingredients: Lactobacillus / algae extract fermentation product, Dunaliella salina extract, Buddleja officinalis extract, lactoferrin and glyceryl glucoside.

[0009] Preferably, the composition comprises, by weight parts, the following components: 0.2-10 parts of Lactobacillus / algae extract fermentation product, 0.2-5 parts of Dunaliella salina extract, 0.0015-5 parts of Buddleja officinalis extract, 0.0006-2 parts of lactoferrin, and 0.006-12 parts of glycerol glucoside.

[0010] More preferably, the composition comprises, by weight parts, the following components: 2-6 parts of Lactobacillus / algae extract fermentation product, 0.5-3 parts of Dunaliella salina extract, 0.5-3 parts of Buddleja officinalis extract, 0.1-1 parts of lactoferrin, and 2-8 parts of glycerol glucoside.

[0011] More preferably, the composition comprises, by weight parts: 4 parts of Lactobacillus / algae extract fermentation product, 1.5 parts of Dunaliella salina extract, 1.5 parts of Buddleja officinalis extract, 0.3 parts of lactoferrin, and 5 parts of glyceryl glucoside.

[0012] Preferably, the composition further contains a solvent.

[0013] In a second aspect, the present invention provides a method for preparing the soothing and repairing composition as described in the first aspect, comprising the following steps: (1) Weigh out the glycerol glucoside according to the ratio, add it to the solvent, and stir until completely dissolved to obtain solution A; (2) Add lactobacillus / algae extract fermentation product, Dunaliella salina extract and Buddleja officinalis extract to solution A obtained in step (1), stir and mix evenly at constant temperature to obtain solution B; (3) After cooling the solution B obtained in step (2) to room temperature, add lactoferrin and stir at low speed until completely dispersed; (4) Filter and sterilize to obtain the soothing and repairing composition.

[0014] Preferably, the amount of solvent used in step (1) is 70-95% of the total mass of the composition; the stirring speed is 200-400 rpm; and the temperature is 25-35℃.

[0015] Preferably, the temperature of the constant temperature stirring in step (2) is 30-40℃, the speed is 300-500rpm, and the time is 20-40min.

[0016] Preferably, the low-speed stirring in step (3) is performed at a speed of 100-200 rpm for 10-20 min.

[0017] Thirdly, the present invention provides the use of the composition as described in the first aspect in the preparation of skin care products with soothing and repairing effects.

[0018] In this invention, the Lactobacillus / algae extract fermentation product is obtained by fermenting algae extracts with Lactobacillus. It is rich in various active ingredients such as polysaccharides, polypeptides, and amino acids, which can regulate the skin microecology, enhance the skin barrier function, and have certain antioxidant and anti-inflammatory activities.

[0019] Dunaliella salina extract is rich in β-carotene, algal polysaccharides and other components, and has excellent antioxidant capacity. It can effectively remove free radicals, reduce UV-induced oxidative damage, promote cell repair and enhance the skin's resilience.

[0020] Buddleja officinalis extract contains active ingredients such as flavonoids and phenylethyl glycosides, which have anti-inflammatory, antioxidant, and mast cell degranulation inhibition effects. It can effectively relieve skin redness, itching and other sensitive symptoms, and has a significant inhibitory effect on inflammatory responses caused by photodamage.

[0021] Lactoferrin is a natural iron-binding glycoprotein with a variety of biological functions, including broad-spectrum antibacterial, anti-inflammatory, and immunomodulatory effects. It can promote the repair of damaged tissues, regulate the skin's immune status, and enhance the skin's resistance to external stimuli.

[0022] Glyceryl glucoside is a natural moisturizing active ingredient with excellent hydrating and moisturizing capabilities. It can increase the water content of the stratum corneum and maintain the integrity of the skin barrier; at the same time, it can protect the cell membrane structure and reduce the damage to cells caused by extreme temperatures.

[0023] The five ingredients work together synergistically to achieve multiple effects such as anti-oxidation, anti-inflammation, and barrier repair, and have significant soothing and repairing effects on both light and temperature damage.

[0024] The beneficial effects of this invention are: 1. This invention scientifically combines fermentation products of Lactobacillus / algae extract, Dunaliella salina extract, Buddleja officinalis extract, lactoferrin and glyceryl glucoside to exert synergistic effects from three dimensions: anti-oxidation, anti-inflammation and barrier repair, which can simultaneously address skin problems caused by photodamage and temperature damage.

[0025] 2. The combination of the components of this invention can significantly improve the survival rate of damaged cells, reduce the secretion level of the inflammatory factor IL-6, and effectively alleviate oxidative stress and inflammatory response.

[0026] 3. The compositions of this invention all use active ingredients that are naturally derived or biocompatible, are gentle and non-irritating to the skin, and are suitable for people with sensitive skin. Detailed Implementation

[0027] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0028] To further illustrate the present invention, detailed descriptions are provided below through the following embodiments. The raw materials used in the following embodiments and comparative examples of the present invention are all commercially available products; the raw material companies listed below represent one of the ways to purchase the raw materials.

[0029] I. Source of Raw Materials The main raw materials used in the embodiments and comparative examples of this invention are sourced from the following sources: Lactobacillus / algae extract fermentation product: purchased from Guangzhou Jucheng Chemical Co., Ltd., in liquid form, with a solid content ≥2%; Dunaliella salina extract: purchased from Guangzhou Jucheng Chemical Co., Ltd., in powder form, with a polysaccharide content ≥30%; Buddleja officinalis extract: purchased from Gansu Fanzhi Pharmaceutical Co., Ltd., in powder form, with a total flavonoid content of ≥5%; Lactoferrin: Purchased from Guangzhou Jucheng Chemical Co., Ltd., in powder form, purity ≥90%; Glyceryl glucoside: purchased from Qingdao Zhongke Lanzhi Biotechnology Development Co., Ltd., in liquid form, with a content of 300 mg / mL; All other reagents are cosmetic grade or analytical grade and can be purchased commercially.

[0030] II. Preparation of Examples and Comparative Examples Example 1 A soothing and repairing composition for photodamage and temperature damage, comprising, by weight, the following components: 4 parts of Lactobacillus / algae extract fermentation product, 1.5 parts of Dunaliella salina extract, 1.5 parts of Buddleja officinalis extract, 0.3 parts of lactoferrin, 5 parts of glyceryl glucoside, and 87.7 parts of purified water.

[0031] The preparation method is as follows: (1) Weigh out the glycerol glucoside according to the ratio, add it to purified water, control the temperature at 30℃, and stir at 300 rpm until completely dissolved to obtain an aqueous solution; (2) Add lactobacillus / algae extract fermentation product, Dunaliella salina extract and Buddleja officinalis extract to the aqueous solution obtained in step (1), control the temperature at 35℃, and stir at 400 rpm for 30 min until the mixture is homogeneous. (3) After cooling the mixture obtained in step (2) to room temperature of 25°C, add lactoferrin and stir at a low speed of 150 rpm for 15 minutes until completely dispersed; (4) The soothing and repairing composition is obtained by filtration and sterilization using a 0.22μm microporous membrane.

[0032] Example 2 A soothing and repairing composition for photodamage and temperature damage, comprising, by weight, the following components: 2 parts of Lactobacillus / algae extract fermentation product, 0.5 parts of Dunaliella salina extract, 0.5 parts of Buddleja officinalis extract, 0.1 parts of lactoferrin, 2 parts of glyceryl glucoside, and 94.9 parts of purified water.

[0033] The preparation method is the same as in Example 1.

[0034] Example 3 A soothing and repairing composition for photodamage and temperature damage, comprising, by weight, the following components: 6 parts of Lactobacillus / algae extract fermentation product, 3 parts of Dunaliella salina extract, 3 parts of Buddleja officinalis extract, 1 part of lactoferrin, 8 parts of glyceryl glucoside, and 79 parts of purified water.

[0035] The preparation method is the same as in Example 1.

[0036] Example 4 A soothing and repairing composition for photodamage and temperature damage, comprising, by weight, the following components: 3 parts of Lactobacillus / algae extract fermentation product, 2 parts of Dunaliella salina extract, 1 part of Buddleja officinalis extract, 0.5 parts of lactoferrin, 6 parts of glyceryl glucoside, and 87.5 parts of purified water.

[0037] The preparation method is the same as in Example 1.

[0038] Example 5 A soothing and repairing composition for photodamage and temperature damage, comprising, by weight, the following components: 8 parts of Lactobacillus / algae extract fermentation product, 4 parts of Dunaliella salina extract, 2 parts of Buddleja officinalis extract, 1.5 parts of lactoferrin, 10 parts of glyceryl glucoside, and 74.5 parts of purified water.

[0039] The preparation method is the same as in Example 1.

[0040] Example 6 A soothing and repairing composition for photodamage and temperature damage, comprising, by weight, the following components: 5 parts of Lactobacillus / algae extract fermentation product, 1 part of Dunaliella salina extract, 2.5 parts of Buddleja officinalis extract, 0.8 parts of lactoferrin, 4 parts of glyceryl glucoside, and 86.7 parts of purified water.

[0041] The preparation method is the same as in Example 1.

[0042] Comparative Example 1 The difference from Example 1 is that no lactobacillus / algae extract fermentation product is added, while the remaining components and amounts are the same as in Example 1, and purified water is added to bring the total to 100 parts. The preparation method is the same as in Example 1.

[0043] Comparative Example 2 The difference from Example 1 is that Dunaliella salina extract is not added, while the remaining components and amounts are the same as in Example 1, and purified water is added to bring the total to 100 parts. The preparation method is the same as in Example 1.

[0044] Comparative Example 3 The difference from Example 1 is that no Buddleja officinalis extract was added, while the remaining components and amounts remained the same as in Example 1, and purified water was added to bring the total to 100 parts. The preparation method was the same as in Example 1.

[0045] Comparative Example 4 The difference from Example 1 is that lactoferrin is not added, while the remaining components and amounts are the same as in Example 1, and purified water is added to bring the total to 100 parts. The preparation method is the same as in Example 1.

[0046] Comparative Example 5 The difference from Example 1 is that glycerol glucoside is not added, while the remaining components and amounts are the same as in Example 1, and purified water is added to bring the total to 100 parts. The preparation method is the same as in Example 1.

[0047] III. Efficacy Testing Experimental Example 1: Detection of the protective effect of UVB photodamage on cells 1. Test Methods A UVB photodamage model was established using immortalized human epidermal keratinocytes (HaCaT). HaCaT cells in the logarithmic growth phase were used at a rate of 5 × 10⁻⁶ cells / year. 4 Inoculate at a density of 100 μL / mL into 96-well plates and incubate at 37°C in a 5% CO2 incubator for 24 h.

[0048] After discarding the original culture medium and washing twice with PBS, all cell groups except the normal control group were irradiated with UVB at a dose of 30 mJ / cm². After irradiation, the normal control group and the model group were given fresh complete culture medium, while the drug-treated groups were given complete culture medium containing 1% of the samples from each example and comparative example, and cultured for another 24 h.

[0049] Cell viability was detected using the CCK-8 assay: 10 μL of CCK-8 solution was added to each well, and after incubation for 2 h, the absorbance (OD value) of each well was measured at 450 nm using a microplate reader. Cell viability was calculated using the formula: "Cell viability (%) = (OD value of drug-treated group / OD value of normal control group) × 100%". Each group had 6 replicates, and the experiment was repeated 3 times. Results are expressed as mean ± standard deviation.

[0050] 2. Test Results Table 1. Cell viability results Group Cell viability (%) normal control group 100.00±2.31 Model group 42.37±3.15aa Example 1 82.56±2.87cc Example 2 71.23±3.42cc Example 3 76.89±2.95cc Example 4 78.41±3.08cc Example 5 73.62±3.51cc Example 6 75.18±2.76cc Comparative Example 1 56.24±3.28bb Comparative Example 2 59.71±3.63bb Comparative Example 3 58.35±3.19bb Comparative Example 4 61.47±2.92bb Comparative Example 5 54.89±3.47bb Note: bb indicates that the comparative example group is significantly different from Example 1, P < 0.01; aa indicates that the model group is significantly different from the normal group, P < 0.01; cc indicates that the example group is significantly different from the model group, P < 0.01.

[0051] The results in the table above show that the cell survival rate in the model group was significantly lower than that in the normal control group (P<0.01), indicating that the UVB photodamage model was successfully established. The cell survival rates in each example group were significantly higher than those in the model group (P<0.01), demonstrating that the composition of this invention has a significant protective effect against UVB-induced HaCaT cell photodamage. Example 1 showed the best effect, with a cell survival rate of 82.56%.

[0052] Although each comparative group also showed a certain cell protection effect, the effect was significantly weaker than that of the example group, indicating that the five components of the present invention have a synergistic effect after being combined, and the absence of any one component will lead to a significant decrease in efficacy.

[0053] Experimental Example 2: Detection of Cell Protective Effect Against Heat Damage 1. Test Methods A heat injury model was established using HaCaT cells. HaCaT cells in the logarithmic growth phase were injected with 5 × 10⁻⁶ cells. 4 Inoculate at a density of 100 μL / mL into 96-well plates and incubate at 37°C in a 5% CO2 incubator for 24 h.

[0054] Except for the normal control group, cells in all other groups were heat-shocked in a 43℃ incubator for 1 hour to establish a heat injury model. After the heat shock, the normal control group and the model group were replaced with fresh complete culture medium, while the drug-treated groups were replaced with complete culture medium containing 1% of the samples from each example and comparative example, and then returned to a 37℃ incubator for another 24 hours.

[0055] Cell viability was detected using the CCK-8 assay, following the same method as in Example 1. Each group had 6 replicates, and the experiment was repeated 3 times. Results are expressed as mean ± standard deviation.

[0056] 2. Test Results Table 2 Cell viability results Group Cell viability (%) normal control group 100.00±2.15 Model group 38.92±2.87aa Example 1 79.34±3.12cc Example 2 67.85±2.96cc Example 3 72.41±3.35cc Example 4 74.68±2.78cc Example 5 69.53±3.21cc Example 6 71.27±3.04cc Comparative Example 1 51.36±2.89bb Comparative Example 2 53.72±3.17bb Comparative Example 3 52.84±2.93bb Comparative Example 4 55.61±3.08bb Comparative Example 5 49.78±3.25bb Note: bb indicates that the comparative example group is significantly different from Example 1, P < 0.01; aa indicates that the model group is significantly different from the normal group, P < 0.01; cc indicates that the example group is significantly different from the model group, P < 0.01.

[0057] The results in the table above show that the cell survival rate in the model group was significantly reduced after heat shock treatment (P<0.01), indicating that the heat damage model was successfully established. All example groups significantly improved the survival rate of HaCaT cells after heat damage (P<0.01), demonstrating that the composition of this invention has a good protective effect against temperature-induced cell damage. Example 1 showed the best effect, with a cell survival rate of 79.34%.

[0058] The cell protection effects of each comparative group were weaker than those of the example group, further verifying the synergistic effect of the five components.

[0059] Experimental Example 3: Detection of IL-6 secretion levels (an inflammatory cytokine) 1. Test Methods HaCaT cells were used at a rate of 2 × 10 5 Inoculate 2 mL of the sample at a density of 10 cells / mL into 6-well plates and incubate for 24 h to establish a UVB photodamage model (irradiation dose 30 mJ / cm²). 2 After irradiation, the drug administration groups were given culture medium containing 1% of the samples from each example and comparative example, while the normal control group and the model group were given an equal amount of fresh culture medium, and the groups were cultured for another 24 hours.

[0060] Cell supernatants were collected from each group, and the interleukin-6 (IL-6) content was detected using an ELISA kit according to the manufacturer's instructions. Each group had three replicates, and the experiment was repeated three times. Results are expressed as mean ± standard deviation.

[0061] 2. Test Results Table 3. Levels of the inflammatory factor IL-6 Group IL-6 level (pg / mL) normal control group 28.64±3.21 Model group 187.52±12.46aa Example 1 68.37±5.82cc Example 2 95.41±7.35cc Example 3 82.16±6.47cc Example 4 76.59±5.93cc Example 5 89.73±7.12cc Example 6 84.25±6.58cc Comparative Example 1 142.68±9.73bb Comparative Example 2 135.29±8.96bb Comparative Example 3 138.74±9.21bb Comparative Example 4 128.56±8.47bb Comparative Example 5 148.31±10.15bb Note: bb indicates that the comparative example group is significantly different from Example 1, P < 0.01; aa indicates that the model group is significantly different from the normal group, P < 0.01; cc indicates that the example group is significantly different from the model group, P < 0.01.

[0062] The results in the table above show that the IL-6 content in the cell supernatant of the model group increased significantly after UVB irradiation (P<0.01), indicating that photodamage triggered a strong inflammatory response. All example groups significantly reduced the secretion level of IL-6 (P<0.01), with Example 1 showing the most significant anti-inflammatory effect, reducing the IL-6 content to 68.37 pg / mL.

[0063] Although the comparative groups could also reduce IL-6 levels to some extent, the effect was far less than that of the example group, indicating that the synergistic effect of the five components can more effectively inhibit photodamage-induced inflammatory response.

[0064] Experiment 4: Effects of HSP47 and HSP70 gene expression in HSF cells 1. Principle Long-wave ultraviolet (UVA) radiation is also a key environmental factor inducing photodamage and photoaging of the skin. It can cause oxidative stress, damage to cell structure and function, and other pathological effects on human skin fibroblasts (HSF). Heat shock proteins (HSPs) are core molecular chaperone proteins synthesized by cells in response to stress stimuli. Among them, HSP70 can maintain homeostasis by repairing and degrading denatured proteins in cells, while HSP47 is involved in collagen synthesis and folding and skin tissue repair. UVA irradiation significantly regulates the gene expression levels of these two proteins in HSF cells. Therefore, the experiment first constructed a UVA-induced HSF cell photodamage model to simulate skin photodamage. The physiological and pathological processes were analyzed. Subsequently, real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) technology was used. First, mRNA in total cellular RNA was reverse transcribed into complementary DNA (cDNA) using reverse transcriptase. Then, using cDNA as a template, PCR amplification was performed using specific primers for HSP47 and HSP70 genes. The amplification process was monitored in real time by fluorescence signals. Finally, combined with internal reference gene correction, the expression level of the target gene was relatively quantitatively analyzed to clarify the regulatory role of the test sample on the expression of heat shock protein genes in UVA-induced HSR cells and to evaluate its potential efficacy in the protection and repair of skin photodamage.

[0065] 2. Experimental Methods The effects of the sample on the HSP47 and HSP70 genes of HSF cells were investigated. The suspension was cultured at 37°C in a 5% CO2 incubator for 24 h. HSF cells that had reached the logarithmic growth phase were then cultured at 2.5 × 10⁻⁶ cells / mL. 5Cells were seeded at a density of 2 mL / well in 6-well culture plates. Cells were grouped according to appropriate concentrations based on CCK-8 results, with three replicates per group. Pretreatment lasted 24 hours. A UVA damage model was established by UVA irradiation, and the same concentration of sample culture medium was added again for 24 hours of further culture. The culture medium was discarded, and the cells were washed once with PBS. An appropriate amount of RNA extraction reagent was added to fully lyse the cells. The extracted RNA was dissolved in an appropriate amount of DNase-free / RNase-free ultrapure distilled water. The extracted RNA was added to a reaction system for reverse transcription to cDNA using an RNA reverse transcription reagent. A qPCR reaction system was prepared, and real-time quantitative PCR was performed on a real-time PCR (qPCR) instrument to detect the Ct values ​​of HSP47, HSP70 genes, and the internal reference gene GAPDH in the sample. The Ct method was used to compare the relative differences in expression levels between the experimental group and the negative control group.

[0066] 3. Experimental Results Table 4. Effects of HSP47 and HSP70 gene expression Group concentration relative expression level of HSP47 gene relative expression level of HSP70 gene normal control group / 1±0.05 1±0.02 Model group / 0.28±0.01aa 3.76±0.04aa Example 1 1% 0.96±0.01cc 1.41±0.01cc Example 2 1% 0.82±0.02cc 1.85±0.02cc Example 3 1% 0.88±0.02cc 1.62±0.02cc Example 4 1% 0.91±0.04cc 1.53±0.01cc Example 5 1% 0.85±0.02cc 1.76±0.02cc Example 6 1% 0.87±0.02cc 1.68±0.02cc Comparative Example 1 1% 0.61±0.04bb 2.84±0.04bb Comparative Example 2 1% 0.65±0.01bb 2.67±0.03bb Comparative Example 3 1% 0.63±0.02bb 2.75±0.06bb Comparative Example 4 1% 0.68±0.01bb 2.59±0.03bb Comparative Example 5 1% 0.59±0.05bb 2.91±0.05bb Note: bb indicates that the comparative example group is significantly different from Example 1, P < 0.01; aa indicates that the model group is significantly different from the normal group, P < 0.01; cc indicates that the example group is significantly different from the model group, P < 0.01.

[0067] 4. Results Analysis Compared with the normal control group, the relative expression level of HSP47 gene in HSF cells of the model group was significantly downregulated to 0.28, and the relative expression level of HSP70 gene was significantly upregulated to 3.76, indicating that UVA irradiation successfully induced photodamage in HSF cells. The expression levels of HSP47 and HSP70 in each example group were significantly higher than those in the model group. Among them, Example 1 showed the best effect, with HSP47 expression level recovering to 0.96 and HSP70 expression level returning to 1.41, close to the level of the normal control group. This indicates that the composition of the present invention can significantly restore the collagen synthesis and tissue repair capacity of damaged cells. The expression level of HSP47 in the comparative example group was only 0.59-0.68, far lower than 0.96 in Example 1; the expression level of HSP70 remained at 2.59-2.91, significantly higher than 1.41 in Example 1. The results clearly demonstrate that the five components—Lactobacillus / algae extract fermentation product, Dunaliella salina extract, Buddleja officinalis extract, lactoferrin, and glyceryl glucoside—have a significant synergistic effect when combined. The absence of any one of the core components will lead to a significant decrease in the gene regulation and repair efficacy against photodamaged cells.

[0068] Experimental Example 5: Human Skin Patch Test 1. Test Methods Thirty healthy volunteers, aged 18-45 years, with no history of skin diseases and no use of hormonal drugs within the past month, were selected and met the inclusion criteria. A closed patch test was used. 0.02 mL of the composition prepared in Example 1 was added to a patch applicator and applied to the inner forearm of the subjects. The patch applicator was removed after 24 hours, and skin reactions were observed at 0.5 h, 24 h, and 48 h after removal. Adverse skin reactions were assessed according to the grading criteria in Table 5.

[0069] Table 5 degree of reaction Rating levels Skin reaction - 0 negative reaction ± 1 Suspicious reaction, only slight erythema + 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and papules may be present. ++ 3 Strong positive reaction (herpes reaction): erythema, infiltration, edema, papules, vesicles; the reaction may extend beyond the test area. +++ 4 Extremely positive reaction (confluent herpes simplex reaction): marked erythema, severe infiltration, edema, confluent herpes simplex; reaction extends beyond the test area. 2. Test Results Table 6 Observation time Level 0 Level 1 Level 2 Level 3 Level 4 0.5h 30 0 0 0 0 24h 30 0 0 0 0 48h 30 0 0 0 0 The results showed that no adverse reactions such as skin erythema, papules, or edema occurred in any of the 30 subjects at any of the observation time points, indicating that the composition of the present invention has low skin irritation, good safety, and is suitable for application in cosmetics.

[0070] Experimental Example 6: Evaluation of the Soothing and Repairing Efficacy in the Human Body 1. Test Methods Thirty volunteers with sensitive skin, aged 20-40, were recruited, with an equal number of men and women. A left-right face control test was conducted. One subject applied 1g of serum base containing 5% of the composition of Example 1 (test side) to one cheek, and 1g of blank base (control side) to the other cheek, once in the morning and once in the evening, for 28 consecutive days.

[0071] The following indicators were tested before use (D0), 14 days after use (D14), and 28 days after use (D28): (1) Skin erythema value: The skin a* value is measured using a skin colorimeter to reflect the degree of skin redness; (2) Transepidermal water loss (TEWL): Detected using a water loss tester, reflecting the skin barrier function; (3) Skin moisture content: The moisture content of the stratum corneum was measured using a skin moisture meter.

[0072] The improvement of subjective discomfort symptoms such as skin burning, stinging, and itching during the subjects' use was also recorded.

[0073] 2. Test Results (1) Changes in skin erythema values Table 7 Group D0 D14 D28 test side 12.84±1.52 10.37±1.28 8.65±1.13** control side 12.79±1.48 12.15±1.36 11.82±1.41 Note: ** indicates that the difference between the experimental side and the control side is extremely significant, P < 0.01.

[0074] (2) Changes in transepidermal water loss (g / (m) 2 ·h)) Table 8 Group D0 D14 D28 test side 28.76±3.42 22.53±2.87 17.94±2.35** control side 28.69±3.38 27.15±3.12 26.48±3.06 Note: ** indicates that the difference between the experimental side and the control side is extremely significant, P < 0.01.

[0075] (3) Changes in the water content of the stratum corneum of the skin (%) Table 9 Group D0 D14 D28 test side 24.37±3.15 31.82±3.64 38.56±4.02** control side 24.29±3.08 26.15±3.27 27.43±3.41 Note: ** indicates that the difference between the experimental side and the control side is extremely significant, P < 0.01.

[0076] The results above show that after 28 days of continuous use, the erythema value of the test side skin was significantly reduced, the TEWL (transepidermal water loss) decreased significantly, and the stratum corneum moisture content was significantly increased, with statistically significant differences compared to the control side. This indicates that the composition of the present invention can effectively improve skin redness, repair skin barrier function, enhance skin's water retention capacity, and has good soothing and repairing effects.

[0077] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A soothing and repairing composition for photodamage and temperature damage, characterized in that, The composition contains the following ingredients: Lactobacillus / algae extract fermentation product, Dunaliella salina extract, Buddleja officinalis extract, lactoferrin, and glyceryl glucoside.

2. The composition according to claim 1, characterized in that, The composition comprises, by weight parts, the following components: 0.2-10 parts of Lactobacillus / algae extract fermentation product, 0.2-5 parts of Dunaliella salina extract, 0.0015-5 parts of Buddleja officinalis extract, 0.0006-2 parts of lactoferrin, and 0.006-12 parts of glycerol glucoside.

3. The composition according to claim 2, characterized in that, The composition comprises, by weight parts, the following components: 2-6 parts of Lactobacillus / algae extract fermentation product, 0.5-3 parts of Dunaliella salina extract, 0.5-3 parts of Buddleja officinalis extract, 0.1-1 parts of lactoferrin, and 2-8 parts of glyceryl glucoside.

4. The composition according to claim 3, characterized in that, The composition comprises, by weight parts, the following components: 4 parts of Lactobacillus / algae extract fermentation product, 1.5 parts of Dunaliella salina extract, 1.5 parts of Buddleja officinalis extract, 0.3 parts of lactoferrin, and 5 parts of glyceryl glucoside.

5. The composition according to claim 1, characterized in that, The composition also contains a solvent.

6. A method for preparing the soothing and repairing composition according to any one of claims 1-5, characterized in that, Includes the following steps: (1) Weigh out the glycerol glucoside according to the ratio, add it to the solvent, and stir until completely dissolved to obtain solution A; (2) Add lactobacillus / algae extract fermentation product, Dunaliella salina extract and Buddleja officinalis extract to solution A obtained in step (1), stir and mix evenly at constant temperature to obtain solution B; (3) After cooling the solution B obtained in step (2) to room temperature, add lactoferrin and stir at low speed until completely dispersed; (4) Filter and sterilize to obtain the soothing and repairing composition.

7. The preparation method according to claim 6, characterized in that, The amount of solvent used in step (1) is 70-95% of the total mass of the composition; the stirring speed is 200-400 rpm and the temperature is 25-35℃.

8. The preparation method according to claim 6, characterized in that, The temperature of the constant temperature stirring in step (2) is 30-40℃, the speed is 300-500rpm, and the time is 20-40min.

9. The preparation method according to claim 6, characterized in that, The low-speed stirring in step (3) is performed at a speed of 100-200 rpm for 10-20 min.

10. The use of the composition according to any one of claims 1-5 in the preparation of a skin care product having soothing and repairing effects.