Yin-nourishing and lung-clearing dust-removing microbial fermentation oral liquid and preparation method thereof

Abstract

The application provides a yin-nourishing and lung-clearing dust-removing microbial fermentation oral liquid and a preparation method thereof, relates to the cross-application technical field of traditional Chinese medicine and microbial fermentation biotechnology, and is prepared from a characteristic medicine-food homologous matrix, a classic yin-nourishing and lung-clearing traditional Chinese medicine component and a fermentation nutrient substrate through microbial fermentation; the application organically combines the traditional yin-nourishing and lung-moistening effect, the powerful antioxidant lung-protecting effect of microbial fermentation and the bacterial cellulose targeted dust-removing and anti-fibrosis function, realizes multi-target point intervention on lung yin deficiency, oxidative stress damage and lung fibrosis process caused by dust, fills the technical blank that existing yin-nourishing and lung-clearing traditional Chinese medicines do not have the function of active elimination of alveolar free silicon dioxide dust, compares the fermentation effects of different strains, different medicine pretreatment methods and different fermentation periods through systematic gradient experiments, and selects the optimal strain, process and period combination, which has strong batch stability and is suitable for industrial mass production.

Description

Technical Field

[0001] This invention relates to the technical field of interdisciplinary application of traditional Chinese medicine and microbial fermentation biotechnology, and in particular to a microbial fermented oral liquid for nourishing yin, clearing the lungs and removing dust, and its preparation method. Background Technology

[0002] Pneumoconiosis and silicosis are irreversible occupational diseases caused by long-term inhalation of free silica dust. Their pathological mechanisms are centered on lung yin deficiency, lung dryness, excessive oxidative stress, chronic persistent inflammation, and progressive interstitial fibrosis of the lungs. Currently, there are no specific drugs for removing free silica dust in clinical practice.

[0003] Traditional Chinese medicine decoctions for nourishing Yin and clearing the lungs have the following technical defects in clinical application: (1) The dissolution rate of active ingredients is low, and the effective ingredients such as flavonoids and polyphenols in the cell walls of Chinese medicinal plants are difficult to be fully released into the aqueous phase, resulting in low bioavailability; (2) The antioxidant activity is weak, and the ability to clear excessive free radicals induced by dust is limited, and it cannot effectively block the continuous damage of oxidative stress to lung tissue; (3) It does not have the function of actively clearing free silica dust in the alveoli, and can only relieve surface symptoms, but cannot delay or block the fibrosis process; (4) It tastes bitter, and patients have poor compliance with long-term use, which is not conducive to daily preventive use by people with occupational exposure.

[0004] Microbial fermentation technology for traditional Chinese medicine utilizes extracellular enzyme systems of microorganisms to degrade macromolecules in traditional Chinese medicine, promoting the release of active small molecules and thus enhancing the antioxidant activity and bioavailability of extracts. However, existing fermentation technologies for traditional Chinese medicine often employ single strains, fixed herbal treatment methods, and fixed fermentation cycles, lacking systematic comparative studies on the fermentation effects of different strains, different herbal pretreatment methods, and different fermentation durations. This makes it impossible to achieve the optimal synergistic effect of antioxidant activity, enrichment of active ingredients, and product stability. Therefore, this invention proposes a microbial fermented oral liquid for nourishing Yin, clearing the lungs, and expelling dust, along with its preparation method, to address the aforementioned problems. Summary of the Invention

[0005] To address the aforementioned issues, this invention proposes a lung-nourishing, dust-clearing, and microbial fermented oral liquid and its preparation method. This invention organically combines the traditional lung-nourishing and yin-moistening effects, the potent antioxidant and lung-protecting effects of microbial fermentation, and the targeted dust-clearing and anti-fibrotic functions of bacterial cellulose. It achieves multi-target intervention on lung yin deficiency, oxidative stress damage, and the progression of pulmonary fibrosis caused by dust, filling the technological gap in existing lung-nourishing and yin-clearing Chinese medicines that do not possess the function of actively clearing free silica dust from the alveoli.

[0006] To achieve the objectives of this invention, the invention is implemented through the following technical solutions:

[0007] A microbial fermented oral liquid for nourishing Yin, clearing the lungs, and removing dust comprises, by weight and volume, a special Yin-nourishing and lung-clearing medicinal and edible homologous matrix composed of 2.5 parts by weight of Luo Han Guo (monk fruit), 4 parts by weight of Shuang Sang Ye (mulberry leaf), 5 parts by weight of Lespedeza bicolor (lestip leaf), and 2.5 parts by weight of Gynostemma pentaphyllum; a classic Yin-nourishing and lung-clearing core Chinese herbal medicine component composed by weight and volume, consisting of 10-16 parts by weight of Sheng Di Huang (rehmannia root), 15-22 parts by weight of Mai Dong (ophiopogon root), 12-18 parts by weight of Xuan Shen (scrophularia root), 12-18 parts by weight of Bei Sha Shen (adenophora root), 10-15 parts by weight of Huang Qi (astragalus root), and 6-10 parts by weight of Chuan Bei Mu (Fritillaria bulb); a fermented nutrient base composed by weight and volume, consisting of 50 parts by weight of white sugar, 50 parts by weight of Huang Li (Chinese pear), and 1000 parts by volume of purified water; and a fermentation strain composed of one or two of the following: kombucha complex, lactic acid bacteria complex, and yeast complex, selected adaptively according to the therapeutic purpose.

[0008] A method for preparing a microbial fermented oral liquid for nourishing Yin, clearing the lungs, and removing dust includes the following steps:

[0009] Step 1: The special Yin-nourishing and lung-clearing medicinal and edible homologous matrix and the classic Yin-nourishing and lung-clearing core Chinese medicine components are pulverized or not pulverized to obtain the first raw material;

[0010] Step 2: Crush or leave the white sugar and yellow pear as raw materials to obtain the second raw material;

[0011] Step 3: Combine the first and second raw materials for pretreatment, then sterilize at 121℃ and cool to 29℃. Finally, inoculate with fermentation bacteria and ferment in the dark for 5-9 days.

[0012] Step 4: Centrifuge, filter, and aseptically fill the fermentation product from Step 3 to obtain the finished oral liquid.

[0013] A further improvement is made in the following step: When performing the compound pretreatment on the first and second raw materials in step three, if the first raw material is a special yin-nourishing and lung-clearing medicinal and edible homologous matrix and a classic yin-nourishing and lung-clearing core Chinese medicine component obtained by non-pulverization, and if the second raw material is white sugar and yellow pear obtained by non-pulverization, the specific operation of the compound pretreatment is as follows: the special yin-nourishing and lung-clearing medicinal and edible homologous matrix and the classic yin-nourishing and lung-clearing core Chinese medicine component, which are the first raw materials, are soaked in purified water for 30 minutes. Then, the white sugar and yellow pear of the second raw material are added and mixed well. Then, sterilization is performed at 121°C and cooled to 29°C. Finally, fermentation is carried out after inoculation with fermentation bacteria and fermented in the dark for 5-9 days.

[0014] The further improvement is that the fermentation strain introduced in step three is a kombucha complex, with an introduction amount of 10%, and the light-proof fermentation process also includes shaking the fermentation equipment at a speed of 120 rpm.

[0015] A further improvement is that when the fermentation strain introduced in step three is a kombucha complex and the fermentation time is 5 days in the dark, the fermentation product prepared and processed in step four to obtain a finished oral liquid can be used to quickly relieve oxidative damage to the lungs in the early stage of silicosis.

[0016] A further improvement is that when the fermentation strain introduced in step three is a kombucha complex and the fermentation time is 9 days in the dark, the fermentation product prepared and processed in step four will result in an oral liquid that can be used to nourish yin and moisten the lungs, resist fibrosis, and expel dust in the late stage of pneumoconiosis and the stage of fibrosis progression.

[0017] A further improvement is made in the following step: When performing the compound pretreatment on the first and second raw materials in step three, if the first raw material is a special yin-nourishing and lung-clearing medicinal and edible homologous matrix and a classic yin-nourishing and lung-clearing core Chinese medicine component obtained by pulverizing, and if the second raw material is white sugar and yellow pear obtained by pulverizing, the specific operation of the compound pretreatment is as follows: the pulverized special yin-nourishing and lung-clearing medicinal and edible homologous matrix and the classic yin-nourishing and lung-clearing core Chinese medicine component are mixed together with the pulverized white sugar and yellow pear, then passed through a 40-mesh sieve, then soaked in purified water for 30 minutes, then decocted and filtered to obtain a clear medicinal liquid, then sterilized at 121°C and cooled to 29°C, and finally inoculated with fermentation bacteria and fermented in the dark for 5-9 days.

[0018] The further improvement is that the fermentation strain introduced in step three is a lactic acid bacteria complex strain, with an introduction amount of 10%. The light-protected fermentation process also includes shaking the fermentation equipment at a speed of 120 rpm and a light-protected fermentation time of 9 days. The fermentation product prepared and processed in step four yields a finished oral liquid used to repair severe dust oxidative lung damage and persistent lung inflammation.

[0019] Further improvements are made in the following: the fermentation strain introduced in step three is a fermentation strain formed by the combination of kombucha complex and yeast complex, with an introduction amount of 10%. The light-proof fermentation process also includes shaking treatment of the fermentation equipment at a shaking speed of 120 rpm and a light-proof fermentation time of 7 days. The fermentation product prepared and processed in step four results in a finished oral liquid for long-term daily preventive use by people working in dusty environments.

[0020] The beneficial effects of this invention are as follows:

[0021] This invention organically combines the traditional effects of nourishing yin and moistening the lungs, the potent antioxidant and lung-protecting effects of microbial fermentation, and the targeted dust removal and anti-fibrotic function of bacterial cellulose. It achieves multi-target intervention on lung yin deficiency, oxidative stress damage, and pulmonary fibrosis caused by dust, filling the technological gap that existing yin-nourishing and lung-clearing Chinese medicines do not have the function of actively removing free silica dust from the alveoli.

[0022] This invention compares the fermentation effects of different strains, different pretreatment methods of medicinal materials, and different fermentation cycles through systematic gradient experiments, and screens out the optimal combination of strains, processes, and cycles. It has strong batch stability and is suitable for industrial mass production.

[0023] The present invention preferably uses an unpulverized and unfiltered medicinal material suspension system combined with kombucha complex fermentation for 9 days. The total flavonoids can reach up to 5.8 mg RE / mL, and the total polyphenols can reach 175 mg GAE / mL. The flavonoid and polyphenol dissolution is 2.7 to 3.2 times higher than that of traditional decoction. The DPPH free radical scavenging rate can reach up to 58.27% after 5 days of fermentation.

[0024] This invention uses a complex of lactic acid bacteria to ferment and pulverize medicinal materials for 9 days, achieving a hydroxyl radical scavenging rate of up to 100%. It has a good repairing effect on severe dust oxidative lung damage and persistent lung inflammation, complementing the DPPH scavenging ability of the unpulverized kombucha system.

[0025] The bacterial cellulose nanofibers generated in situ during the fermentation of the kombucha complex of the present invention have an extremely high specific surface area and can specifically adsorb free silica dust in the alveoli, thus adding a physical dust removal function to traditional Chinese medicine preparations.

[0026] This invention uses a fermentation strain formed by combining kombucha and yeast to ferment pulverized medicinal materials for 7 days. After microbial transformation, the fermentation products are rich in volatile flavor substances, significantly reducing the bitterness of the Chinese medicine and resulting in a sweet and mellow taste. Patient compliance with long-term use is significantly better than with traditional Chinese medicine decoctions. Moreover, there are no chemical additives throughout the process, making it highly safe and suitable for long-term continuous use. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the preparation method of the present invention. Detailed Implementation

[0028] To enhance understanding of the present invention, the present invention will be further described in detail below with reference to embodiments. These embodiments are only used to explain the present invention and do not constitute a limitation on the scope of protection of the present invention.

[0029] Example 1

[0030] according to Figure 1 As shown in the figure, this embodiment proposes a method for preparing a microbial fermented oral liquid for nourishing yin, clearing the lungs, and removing dust, including the following steps:

[0031] Step 1: Take 2.5g of monk fruit, 4g of mulberry leaf, 5g of lespedeza, 2.5g of trifoliate leaf, 13g of rehmannia root, 18g of ophiopogon root, 15g of scrophularia root, 15g of glehnia root, 12g of astragalus root, and 8g of fritillaria bulb without grinding them to obtain the first raw material.

[0032] Step 2: Non-pulverize 50g of white sugar and 50g of yellow pear to obtain the second raw material;

[0033] Step 3: Soak 2.5g of monk fruit, 4g of mulberry leaves, 5g of lespedeza, 2.5g of trifoliate leaves, 13g of rehmannia root, 18g of ophiopogon root, 15g of scrophularia root, 15g of glehnia root, 12g of astragalus root, and 8g of fritillaria bulb in 1000ml of purified water for 30min. Then add 50g of white sugar and 50g of yellow pear and mix well. Sterilize at 121℃ and cool to 29℃. Finally, inoculate with kombucha complex microbial culture at a rate of 10%. Ferment in the dark for 5 days, and shake the fermentation equipment at a speed of 120rpm during the process.

[0034] Step 4: Centrifuge the fermentation product from Step 3 at 8000 rpm for 10 min, filter aseptically at 0.22 μm, and aseptically fill to obtain the finished oral liquid.

[0035] The oral liquid prepared in this embodiment showed a DPPH free radical scavenging rate of 58.27%, and the kombucha complex can generate bacterial cellulose nanofibers of 0.5-1.2 g / L in situ, which can rapidly alleviate oxidative damage to the lungs in the early stages of silicosis.

[0036] Example 2

[0037] according to Figure 1 As shown in the figure, this embodiment proposes a method for preparing a microbial fermented oral liquid for nourishing yin, clearing the lungs, and removing dust, including the following steps:

[0038] Step 1: Take 2.5g of monk fruit, 4g of mulberry leaf, 5g of lespedeza, 2.5g of trifoliate leaf, 13g of rehmannia root, 18g of ophiopogon root, 15g of scrophularia root, 15g of glehnia root, 12g of astragalus root, and 8g of fritillaria bulb without grinding them to obtain the first raw material.

[0039] Step 2: Non-pulverize 50g of white sugar and 50g of yellow pear to obtain the second raw material;

[0040] Step 3: Soak 2.5g of monk fruit, 4g of mulberry leaves, 5g of lespedeza, 2.5g of trifoliate leaves, 13g of rehmannia root, 18g of ophiopogon root, 15g of scrophularia root, 15g of glehnia root, 12g of astragalus root, and 8g of fritillaria bulb in 1000ml of purified water for 30min. Then add 50g of white sugar and 50g of yellow pear and mix well. Sterilize at 121℃ and cool to 29℃. Finally, inoculate with kombucha complex microbial culture at a rate of 10%. Ferment in the dark for 9 days, and shake the fermentation equipment at a speed of 120rpm during the process.

[0041] Step 4: Centrifuge the fermentation product from Step 3 at 8000 rpm for 10 min, filter aseptically at 0.22 μm, and aseptically fill to obtain the finished oral liquid.

[0042] The oral liquid prepared in this embodiment was found to contain 5.8 mg RE / mL of total flavonoids and 175 mg GAE / mL of total polyphenols. The kombucha complex can generate bacterial cellulose nanofibers of 0.5-1.2 g / L in situ. The product can nourish yin and moisten the lungs, resist fibrosis, and expel dust in the late stage of pneumoconiosis and the stage of fibrosis progression.

[0043] Example 3

[0044] according to Figure 1 As shown in the figure, this embodiment proposes a method for preparing a microbial fermented oral liquid for nourishing yin, clearing the lungs, and removing dust, including the following steps:

[0045] Step 1: Grind 2.5g of monk fruit, 4g of mulberry leaf, 5g of lespedeza, 2.5g of trifoliate leaf, 13g of rehmannia root, 18g of ophiopogon root, 15g of scrophularia root, 15g of glehnia root, 12g of astragalus root, and 8g of fritillaria bulb to obtain the first raw material;

[0046] Step 2: Crush 50g of white sugar and 50g of yellow pear to obtain the second raw material;

[0047] Step 3: Mix together the pulverized products of 2.5g of monk fruit, 4g of mulberry leaves, 5g of lespedeza, 2.5g of trifoliate leaves, 13g of rehmannia root, 18g of ophiopogon root, 15g of scrophularia root, 15g of adenophora root, 12g of astragalus root, 8g of fritillaria bulb, 50g of white sugar, and 50g of pear and pass through a 40-mesh sieve. Then soak in 1000 volumes of purified water for 30 minutes, followed by decoction and filtration to obtain a clear liquid. Then sterilize at 121℃ and cool to 29℃. Finally, inoculate with a 10% lactic acid bacteria complex culture and ferment in the dark for 9 days. During the light-protected fermentation process, the fermentation equipment is shaken at a speed of 120 rpm.

[0048] Step 4: Centrifuge the fermentation product from Step 3 at 8000 rpm for 10 min, filter aseptically at 0.22 μm, and aseptically fill to obtain the finished oral liquid.

[0049] The oral liquid prepared in this embodiment showed a 100% scavenging rate of hydroxyl radicals and the best repair effect on severe dust oxidative lung damage and persistent lung inflammation.

[0050] Example 4

[0051] according to Figure 1 As shown in the figure, this embodiment proposes a method for preparing a microbial fermented oral liquid for nourishing yin, clearing the lungs, and removing dust, including the following steps:

[0052] Step 1: Grind 2.5g of monk fruit, 4g of mulberry leaf, 5g of lespedeza, 2.5g of trifoliate leaf, 13g of rehmannia root, 18g of ophiopogon root, 15g of scrophularia root, 15g of glehnia root, 12g of astragalus root, and 8g of fritillaria bulb to obtain the first raw material;

[0053] Step 2: Crush 50g of white sugar and 50g of yellow pear to obtain the second raw material;

[0054] Step 3: Mix together 2.5g of monk fruit, 4g of mulberry leaves, 5g of lespedeza, 2.5g of trifoliate leaves, 13g of rehmannia root, 18g of ophiopogon root, 15g of scrophularia root, 15g of adenophora root, 12g of astragalus root, 8g of fritillaria bulb, 50g of white sugar, and 50g of pear (after pulverizing) and pass through a 40-mesh sieve. Then soak in 1000ml of purified water for 30 minutes. Next, decoct and filter to obtain a clear liquid. Then sterilize at 121℃ and cool to 29℃. Finally, inoculate with a fermentation starter culture formed by combining kombucha and yeast complex cultures at a rate of 10%. Ferment in the dark for 7 days. During the fermentation process, the fermentation equipment is shaken at a speed of 120rpm.

[0055] Step 4: Centrifuge the fermentation product from Step 3 at 8000 rpm for 10 min, filter aseptically at 0.22 μm, and aseptically fill to obtain the finished oral liquid.

[0056] The oral liquid prepared in this embodiment is rich in volatile flavor substances, with a significantly reduced bitterness of traditional Chinese medicine, and a mild sweet and sour taste, making it suitable for long-term daily preventive use by people working in dusty environments. In addition, the kombucha complex can generate bacterial cellulose nanofibers of 0.5-1.2 g / L in situ.

[0057] Comparative Example

[0058] Using the same proportions of medicinal materials (a unique yin-nourishing and lung-clearing medicinal and edible matrix composed of 2.5 parts by weight of Luo Han Guo, 4 parts by weight of Shuang Sang Ye, 5 parts by weight of Lespedeza bicolor and 2.5 parts by weight of Gynostemma pentaphyllum; and a classic yin-nourishing and lung-clearing core Chinese medicine component composed of 10-16 parts by weight of Sheng Di Huang, 15-22 parts by weight of Mai Dong, 12-18 parts by weight of Xuan Shen, 12-18 parts by weight of Bei Sha Shen, 10-15 parts by weight of Huang Qi, and 6-10 parts by weight of Chuan Bei Mu), the sample was prepared by conventional decoction and concentrated twice, without microbial fermentation, and used as an efficacy control sample.

[0059] The embodiments (Examples 1 to 4) and comparative examples of this invention are summarized in Table 1 below:

[0060] Example 1 Example 2 Example 3 Example 4 Comparative Example Pre-processing of medicinal materials No grinding, no filtering No grinding, no filtering The powder was pulverized through a 40-mesh sieve and filtered to obtain a clear liquid. The powder was pulverized through a 40-mesh sieve and filtered to obtain a clear liquid. Uncrushed Composite pretreatment Soak in purified water for 30 minutes Soak in purified water for 30 minutes Soak in purified water for 30 minutes, boil, and filter. Soak in purified water for 30 minutes, boil, and filter. Conventional decoction with secondary concentration Fermentation strains Kombucha complex microbial community Kombucha complex microbial community Lactic acid bacteria complex A blend of kombucha and yeast cultures None (unfermented) Fermentation cycle 5 days 9 days 9 days 7 days none Oscillation speed 120 rpm 120 rpm 120 rpm 120 rpm none sterilization conditions Sterilize at 121℃ for 20 minutes Sterilize at 121℃ for 20 minutes Sterilize at 121℃ for 20 minutes Sterilize at 121℃ for 20 minutes none Key Indicators DPPH removal rate: 58.27% Total flavonoids 5.8 mg RE / mL, total polyphenols 175 mg GAE / mL 100% hydroxyl radical scavenging rate Rich in volatile flavor compounds Flavonoid polyphenols have low solubility and weak antioxidant properties. Efficacy positioning Rapidly relieve oxidative damage to the lungs in the early stages of silicosis Late-stage pneumoconiosis, progressive fibrosis: nourishing yin and moistening the lungs, anti-fibrosis, and dust removal. Repair of severe dust oxidative lung damage and persistent lung inflammation Long-term daily preventative use for people working in dusty environments It only relieves surface symptoms and has no dust removal function.

[0061] As can be seen from Table 1 above:

[0062] The comparative method uses conventional water decoction with secondary concentration, utilizing thermodynamic principles to allow water molecules to permeate into plant cells, relying on high temperature and concentration gradient to passively dissolve the active ingredients. This method cannot break down the dense plant cell walls composed of cellulose, hemicellulose, and pectin; large molecular active substances such as flavonoids and polyphenols are physically trapped within the cell wall skeleton and cannot be fully released. The entire process is purely physical extraction, without any biotransformation. This invention employs a microbial fermentation technology route, utilizing various extracellular enzyme systems such as cellulase, pectinase, and protease secreted by microorganisms during their growth and metabolism to enzymatically break down the cell walls of medicinal plants, allowing the intracellular active ingredients to be fully released into the fermentation broth. Simultaneously, the metabolic activities of the microorganisms convert large molecules into small molecular active ingredients and synthesize new functional substances in situ during fermentation.

[0063] A differentiated dual-process system of pulverization and uncrushing was established. The uncrushing process preserves the complete tissue structure of the medicinal materials, allowing microorganisms to adhere to the surface for continuous metabolism, resulting in higher accumulation of active ingredients and a more stable system. The pulverization process, on the other hand, makes the medicinal solution clear, rich in flavor, and has a significant hydroxyl radical scavenging ability. The two processes can be flexibly selected according to the therapeutic purpose.

[0064] This product employs a differentiated fermentation system incorporating multiple microbial strains, including kombucha, lactic acid bacteria, and yeast. Each strain has a clearly defined function: kombucha primarily provides antioxidant benefits, produces cellulose, and helps cleanse the lungs; lactic acid bacteria primarily combat inflammation and scavenge hydroxyl radicals; and yeast enhances flavor. Through gradient experiments, three peak functional periods are precisely identified: 5 days for peak antioxidant activity, 7 days for optimal flavor, and 9 days for the highest levels of active ingredients. Different fermentation periods can be selected based on the therapeutic purpose. During fermentation, the kombucha complex generates bacterial cellulose nanofibers (0.5–1.2 g / L) in situ. These fibers have an extremely high specific surface area, specifically adsorbing free silica dust within the alveoli, thus achieving lung dust clearance.

[0065] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A microbial fermented oral liquid for nourishing Yin, clearing the lungs, and removing dust, characterized in that: It includes a unique yin-nourishing and lung-clearing medicinal and edible matrix composed of 2.5 parts by weight of monk fruit, 4 parts by weight of mulberry leaves, 5 parts by weight of lespedeza, and 2.5 parts by weight of trifoliate leaves, in a weight-volume ratio. It includes a classic core Chinese herbal medicine composition for nourishing Yin and clearing the lungs, consisting of 10-16 parts by weight of Rehmannia glutinosa, 15-22 parts by weight of Ophiopogon japonicus, 12-18 parts by weight of Scrophularia ningpoensis, 12-18 parts by weight of Adenophora stricta, 10-15 parts by weight of Astragalus membranaceus, and 6-10 parts by weight of Fritillaria cirrhosa, in a weight-volume ratio. It includes a fermented nutrient base consisting of 50 parts by weight of white sugar, 50 parts by weight of yellow pear, and 1000 parts by volume of purified water in a weight-volume ratio. This includes fermentation strains formed by combining one or two of the following: kombucha complex, lactic acid bacteria complex, and yeast complex, according to the therapeutic purpose.

2. A method for preparing a microbial fermented oral liquid for nourishing Yin, clearing the lungs, and expelling dust as described in claim 1, characterized in that, Includes the following steps: Step 1: The special Yin-nourishing and lung-clearing medicinal and edible homologous matrix and the classic Yin-nourishing and lung-clearing core Chinese medicine components are pulverized or not pulverized to obtain the first raw material; Step 2: Crush or leave the white sugar and yellow pear as raw materials to obtain the second raw material; Step 3: Combine the first and second raw materials for pretreatment, then sterilize at 121℃ and cool to 29℃. Finally, inoculate with fermentation bacteria and ferment in the dark for 5-9 days. Step 4: Centrifuge, filter, and aseptically fill the fermentation product from Step 3 to obtain the finished oral liquid.

3. The preparation method of the oral liquid for nourishing yin, clearing the lungs, and expelling dust by microbial fermentation according to claim 2, characterized in that: In step three, when the first and second raw materials undergo compound pretreatment, if the first raw material is a special yin-nourishing and lung-clearing medicinal and edible homologous matrix and a classic yin-nourishing and lung-clearing core Chinese medicine component obtained by non-pulverization, and if the second raw material is white sugar and yellow pear obtained by non-pulverization, the specific operation of the compound pretreatment is as follows: the special yin-nourishing and lung-clearing medicinal and edible homologous matrix and the classic yin-nourishing and lung-clearing core Chinese medicine component, which are the first raw materials, are soaked in purified water for 30 minutes. Then, the white sugar and yellow pear of the second raw material are added and mixed well. Then, sterilization is performed at 121°C and cooled to 29°C. Finally, fermentation is carried out after inoculation with fermentation bacteria and fermented in the dark for 5-9 days.

4. The preparation method of the oral liquid for nourishing yin, clearing the lungs, and expelling dust by microbial fermentation according to claim 3, characterized in that: The fermentation strain introduced in step three is a kombucha complex, with an introduction amount of 10%. The light-proof fermentation process also includes shaking the fermentation equipment at a speed of 120 rpm.

5. The preparation method of the oral liquid for nourishing yin, clearing the lungs, and expelling dust by microbial fermentation according to claim 4, characterized in that: When the fermentation strain introduced in step three is kombucha complex and the fermentation time is 5 days in the dark, the fermentation product prepared and processed in step four yields a finished oral liquid that can be used to quickly relieve oxidative damage to the lungs in the early stages of silicosis.

6. The preparation method of the oral liquid for nourishing yin, clearing the lungs, and expelling dust by microbial fermentation according to claim 4, characterized in that: When the fermentation strain introduced in step three is kombucha complex and the fermentation time is 9 days in the dark, the fermentation product prepared and processed in step four yields a finished oral liquid that is used to nourish yin and moisten the lungs, resist fibrosis, and expel dust in the late stage of pneumoconiosis and the stage of fibrosis progression.

7. The preparation method of the oral liquid for nourishing yin, clearing the lungs, and expelling dust by microbial fermentation according to claim 2, characterized in that: In step three, when the first and second raw materials undergo compound pretreatment, if the first raw material is a special yin-nourishing and lung-clearing medicinal and edible homologous matrix and a classic yin-nourishing and lung-clearing core Chinese medicine component obtained by pulverizing, and if the second raw material is white sugar and yellow pear obtained by pulverizing, the specific operation of the compound pretreatment is as follows: the pulverized special yin-nourishing and lung-clearing medicinal and edible homologous matrix and the classic yin-nourishing and lung-clearing core Chinese medicine component are mixed together with the pulverized white sugar and yellow pear, then passed through a 40-mesh sieve, then soaked in purified water for 30 minutes, then decocted and filtered to obtain a clear medicinal liquid, then sterilized at 121°C and cooled to 29°C, and finally inoculated with fermentation bacteria and fermented in the dark for 5-9 days.

8. The preparation method of the oral liquid for nourishing yin, clearing the lungs, and expelling dust by microbial fermentation according to claim 7, characterized in that: The fermentation strain introduced in step three is a lactic acid bacteria complex strain, with an inoculation amount of 10%. The light-protected fermentation process also includes shaking the fermentation equipment at a speed of 120 rpm and a light-protected fermentation time of 9 days. The fermentation product obtained after processing in step four is used to repair severe dust oxidative lung damage and persistent lung inflammation.

9. The preparation method of the oral liquid for nourishing yin, clearing the lungs, and expelling dust by microbial fermentation according to claim 7, characterized in that: The fermentation strain introduced in step three is a fermentation strain formed by combining kombucha complex and yeast complex, with an introduction amount of 10%. The light-proof fermentation process also includes shaking the fermentation equipment at a speed of 120 rpm and a light-proof fermentation time of 7 days. The fermentation product obtained after processing in step four is a finished oral liquid for long-term daily preventive use by people working in dusty environments.

Images