A silk fibroin solution activated spontaneous electrokinetic permeation medical dressing and a preparation method thereof

Abstract

This invention discloses a silk fibroin solution-activated self-generating permeation-enhancing medical dressing and its preparation method. The medical dressing includes a base fabric layer, a microcurrent generating unit disposed on the base fabric layer, and a silk fibroin aqueous solution packaged independently of the base fabric layer. The microcurrent generating unit includes a first metal electrode and a second metal electrode with different electrochemical potentials. The silk fibroin aqueous solution serves as both the active ingredient to be absorbed transdermally and the electrolyte solution to activate the microcurrent generating unit. When the silk fibroin aqueous solution simultaneously contacts the first and second metal electrodes, a galvanic cell circuit is formed, spontaneously generating a microcurrent. This microcurrent promotes the transdermal absorption of the silk fibroin itself. This invention utilizes the silk fibroin solution as the galvanic cell electrolyte to achieve a closed-loop synergistic system of "self-activation and self-permeation enhancement," significantly improving the transdermal absorption rate of silk fibroin and showing broad application prospects in the field of medical dressings.

Description

Technical Field

[0001] This invention belongs to the field of medical dressings and biomaterials technology, specifically relating to a functional dressing that utilizes a specific biological macromolecule solution to activate and generate a microcurrent, thereby promoting the transdermal absorption of the biological macromolecule itself, and its preparation method. Background Technology

[0002] Silk fibroin (SF) is a natural high-molecular-weight fibrous protein extracted from silkworm silk. It possesses excellent biocompatibility, moisturizing properties, antioxidant effects, and the ability to promote tissue repair, making it a research hotspot in the high-end skincare and medical dressing fields. However, silk fibroin typically has a molecular weight of tens to hundreds of kDa, classifying it as a large molecule. Under conventional application or patch conditions, it is difficult to effectively penetrate the skin's stratum corneum barrier, resulting in low bioavailability and its inability to fully exert its efficacy.

[0003] In existing technologies, researchers have developed various strategies to promote the transdermal absorption of macromolecular active ingredients: one is to degrade silk fibroin into small molecule peptides through hydrolysis or enzymatic hydrolysis, but this results in a partial loss of its original bioactivity; the second is to prepare silk fibroin into nanoparticles or nanomicelles, but the preparation process is complex, and the uniformity and stability of the nanoparticle load in the patch product are difficult to guarantee; the third is to utilize physical penetration enhancement technologies, such as iontophoresis, electroporation, and microcurrent beauty devices. Among these, microcurrent technology has attracted much attention due to its high safety and ease of operation.

[0004] For example, Chinese patent CN112156025A discloses a silver ion-sensing bio-microcurrent membrane cloth, which uses a base cloth layer containing magnetic powder and Ag. 2+ A linear magnetic strip containing a solution, germanium powder, binder, and solubilizer is used after being soaked in water. Under the influence of skin heat conduction, the membrane temperature rises to above 32°C, causing the germanium powder to release electrons and germanium ions, while Ag... 2+ The solution dissolves to form an electrolyte solution, which then generates a microcurrent under the combined action of a magnetic field and electrons, used for skin tightening, whitening, and spot removal. However, the generation of the microcurrent in this technology depends on specific magnetic materials (magnetic powder, germanium powder) and temperature conditions, and its effects are localized to the skin itself, without targeting specific active ingredients for enhanced penetration. Existing technologies do not disclose a method for simultaneously using silk fibroin solution as both an active ingredient and an electrolyte to activate the microcurrent generating unit, and for utilizing this microcurrent to inversely promote the transdermal absorption of silk fibroin.

[0005] Furthermore, Chinese patent CN120571073A discloses a self-generating composite membrane for repairing critical-sized bone defects and its preparation method. It uses silk fibroin-derived peptides to improve electrical output performance. However, this composite membrane is mainly applied to bone defect repair and does not address transdermal absorption and permeation enhancement techniques, nor does it disclose a technical solution that simultaneously uses silk fibroin solution as an activation medium and active ingredient. Chinese patent CN107375994A discloses a medical gel bandage, which involves silk fibroin gel and electrodes, but the electrodes are only used as an auxiliary element in the gel preparation process, not for in-situ permeation enhancement during product use. Chinese patent CN121570715A discloses a self-generating microcurrent stimulation dressing, but it does not involve the technical concept of using the active ingredient itself as an electrolyte solution.

[0006] Therefore, developing an innovative medical dressing that combines highly biocompatible silk fibroin with safe and comfortable microcurrent stimulation to achieve "self-activation and self-penetration" of silk fibroin has significant market value and innovative significance. Summary of the Invention

[0007] The present invention aims to provide a silk fibroin solution-activated self-generating permeation-enhancing medical dressing and its preparation method, in order to solve the problems of low transdermal absorption rate of silk fibroin, lack of spontaneous in-situ permeation-enhancing mechanism, and functional disconnect between microcurrent technology and active ingredients in existing medical dressings.

[0008] To achieve the above-mentioned technical objectives and effects, the present invention is implemented through the following technical solution:

[0009] A silk fibroin solution-activated self-generating permeation-enhancing medical dressing, characterized in that it comprises:

[0010] Base fabric layer;

[0011] Microcurrent generating unit disposed on the base fabric layer;

[0012] And an aqueous solution of silk fibroin packaged independently of the base fabric layer;

[0013] The microcurrent generating unit includes a first metal electrode and a second metal electrode, the first metal electrode and the second metal electrode having different electrochemical potentials;

[0014] The silk fibroin aqueous solution has a mass percentage concentration of 0.5%-2.0% and a pH of 5.5-7.5. The silk fibroin aqueous solution serves as both the active ingredient to be absorbed transdermally and the electrolyte solution to activate the microcurrent generating unit.

[0015] The first metal electrode material is silver or a silver-containing alloy, and the second metal electrode material is zinc or a zinc-containing alloy;

[0016] The distance between the first metal electrode and the second metal electrode is 1.0-2.0 cm;

[0017] When the silk fibroin aqueous solution is used and simultaneously comes into contact with the first metal electrode, the second metal electrode, and human skin, the first metal electrode, the second metal electrode, the silk fibroin aqueous solution, and human skin together form a closed galvanic cell circuit, spontaneously generating a microcurrent of 50-200 microamps. The microcurrent promotes the transdermal absorption of the silk fibroin itself through electroporation and iontophoresis effects.

[0018] Furthermore, the base fabric layer is one of nonwoven fabric, silk fabric, or knitted fabric.

[0019] Furthermore, the microcurrent generating units are arranged on the base fabric layer in mutually insulated radial, grid, or strip patterns.

[0020] Furthermore, the first metal electrode and the second metal electrode are respectively formed by drying and curing conductive slurry; the conductive slurry includes metal powder, conductive carbon powder, binder and solubilizer, wherein the metal powder of the first metal electrode is silver powder and the metal powder of the second metal electrode is zinc powder; the binder is selected from one or more of acrylic polymer, silicone or polyurethane, and the binder accounts for 40%-65% of the total mass of the slurry; the solubilizer is propylene glycol.

[0021] This invention also provides a method for preparing the above-mentioned silk fibroin solution-activated self-generating permeation-enhancing medical dressing, comprising the following steps:

[0022] a) Preparation of silk fibroin aqueous solution: Dissolve silk fibroin in purified water to obtain a silk fibroin aqueous solution with a mass percentage concentration of 0.5%-2.0% and a pH of 5.5-7.5;

[0023] b) Preparation of microcurrent medical dressing: A first metal electrode and a second metal electrode are formed on a base fabric layer by screen printing. The first metal electrode and the second metal electrode are formed by drying and curing a conductive paste. The conductive paste contains metal powder, conductive carbon powder, binder and solubilizer. The metal powder of the first metal electrode is silver powder and the metal powder of the second metal electrode is zinc powder. The binder is selected from one or more of acrylic polymer, silicone or polyurethane. The binder accounts for 40%-65% of the total mass of the paste. The solubilizer is propylene glycol.

[0024] c) Separate the silk fibroin aqueous solution from step a) and the microcurrent medical dressing from step b) into separate compartments or bags.

[0025] Furthermore, the present invention also provides the application of the above-mentioned silk fibroin solution-activated self-generating permeation-enhancing medical dressing in the preparation of medical dressings with repair functions.

[0026] It should be noted that the first metal electrode of this invention is made of silver or a silver-containing alloy, and the second metal electrode is made of zinc or a zinc-containing alloy. The silver-zinc electrode pair has a suitable electrochemical potential difference, enabling a stable output of 50-200 microamps of microcurrent in silk fibroin aqueous solution (pH 5.5-7.5). Simultaneously, the zinc ions released from the dissolved zinc anode exhibit antibacterial activity within a suitable range, and the silver electrode demonstrates excellent biocompatibility. This invention has determined the silver-zinc electrode combination through extensive experimental screening. This combination ensures sufficient microcurrent intensity while maintaining biocompatibility, avoiding the shortcomings of other metal combinations (such as the copper-silver combination, which suffers from excessively low output current due to copper surface passivation, and the zinc-iron combination, which poses safety risks due to excessive zinc dissolution). Therefore, this invention explicitly limits the electrode material to a silver / zinc combination to ensure the optimal balance between microcurrent intensity and biocompatibility.

[0027] Compared with the prior art, the present invention has the following significant advantages:

[0028] (1) For the first time, silk fibroin aqueous solution is simultaneously defined as both "active ingredient" and "activation medium," constructing a closed-loop transdermal delivery system that is "self-activated and self-promoting." In existing technologies, the activation medium of microcurrent medical patches is mostly ordinary water, sweat, or electrolyte-containing essence, and its penetration-enhancing target is usually other active ingredients. The activation medium and the active ingredient to be enhanced have never been unified as silk fibroin. This design has significant novelty.

[0029] (2) This invention utilizes the inherent ionic conductivity of the silk fibroin solution (silk fibroin molecules are negatively charged at pH 5.5-7.5 and contain trace amounts of salt ions) to make it an electrolyte solution for a galvanic cell, solving the problem of requiring additional electrolytes in traditional galvanic cell applications. Simultaneously, the generated microcurrent actively propels the silk fibroin macromolecules through the stratum corneum via electroporation and iontophoresis, overcoming the technical bottleneck of difficult transdermal absorption of silk fibroin. This solution is not a simple functional superposition but rather a synergistic and innovative concept.

[0030] (3) The product adopts a dry storage and activation method, which has good stability and is easy to use; no external power supply is required, and it is safe and non-irritating; the electrode materials are made of biocompatible materials such as silver and zinc, and the cost is controllable; the microcurrent medical dressing preparation process is mature and easy to industrialize.

[0031] (4) In vitro transdermal experiments have confirmed that after using the silk fibroin solution activated self-generating permeation medical dressing of the present invention, the penetration depth and retention of silk fibroin in the skin are significantly higher than those of ordinary silk fibroin dressing, and the repair effect is correspondingly enhanced.

[0032] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0033] Figure 1 This is a schematic diagram illustrating the working principle of the silk fibroin solution-activated self-generating permeation-enhancing medical dressing of the present invention.

[0034] Figure 2 This is a comparison chart of the MTT cell proliferation experiment results of Examples 1-3 and Comparative Examples 1-2 of the present invention. Detailed Implementation

[0035] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the embodiments. The described embodiments are only some embodiments of this application, and not all of them. The present invention is not limited to the following embodiments. Unless otherwise specified, the technical means used in the following embodiments are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following embodiments can be obtained commercially unless otherwise specified.

[0036] The silk fibroin described in this invention was purchased from Baiyimeiheng (Beijing) Technology Co., Ltd.

[0037] Terminology Explanation: In this invention, "self-activation" refers to the silk fibroin aqueous solution simultaneously activating the microcurrent generating unit as an electrolyte solution, without the need for additional electrolyte addition; "self-penetration enhancement" refers to the microcurrent generated by the activation of the silk fibroin aqueous solution being used in reverse to promote the transdermal absorption of the silk fibroin itself; "transdermal absorption" broadly refers to the total amount of active ingredients entering the stratum corneum and epidermis of the skin. In in vitro transdermal experiments, the specific detection index is the amount retained in the skin tissue (i.e., skin retention), which is more suitable for evaluating the local efficacy of patch-type products.

[0038] like Figure 1 As shown, the working principle of this invention is as follows: When the silk fibroin aqueous solution simultaneously contacts the first metal electrode (positive electrode, silver electrode) and the second metal electrode (negative electrode, zinc electrode), the ions in the silk fibroin aqueous solution act as electrolytes, forming an ionic conductive pathway between the two electrodes. Simultaneously, an electronic conductive pathway is formed between the two electrodes through an external circuit (skin / tissue), thus constituting a closed galvanic cell circuit and spontaneously generating a microcurrent. This microcurrent increases the permeability of the stratum corneum lipid bilayer through electroporation, and simultaneously drives negatively charged silk fibroin molecules to migrate directionally into the skin through iontophoresis, ultimately achieving enhanced transdermal absorption of the silk fibroin itself.

[0039] Example 1

[0040] I. Material Preparation

[0041] Conductive pastes: silver conductive paste (containing 50% silver powder, 5% conductive carbon powder, 43% acrylic polymer binder, and 2% propylene glycol solubilizer); zinc conductive paste (containing 45% zinc powder, 5% conductive carbon powder, 48% polyurethane binder, and 2% propylene glycol solubilizer).

[0042] Silk fibroin aqueous solution: Dissolve silk fibroin in purified water to prepare a solution with a mass percentage concentration of 2.0%, adjust the pH to 6.5 with 0.1 mol / L NaOH, filter and sterilize with a 0.22 μm filter membrane, and fill into aluminum foil bags.

[0043] Base fabric layer: nonwoven fabric.

[0044] II. Preparation of Microcurrent Medical Adhesives

[0045] Fabrication of the microcurrent medical dressing: Symmetrical silver electrode areas (positive electrode) and zinc electrode areas (negative electrode) were printed on a nonwoven fabric using screen printing, with a 1.5 cm insulating gap between the two electrode areas. The dressing was then dried at 100℃ for 10 minutes to cure the electrodes. The electrode pattern consisted of radial lines, covering the forehead, cheeks, and chin areas.

[0046] Packaging: The prepared dried microcurrent medical dressing and the silk fibroin aqueous solution are sealed in separate compartments in the same outer packaging.

[0047] III. Effect Test

[0048] In vitro transdermal experiments were conducted using a Franz diffusion cell. Experimental conditions: ambient temperature 25±2℃, relative humidity 40%-60%, and effective diffusion area of ​​the Franz diffusion cell 2.0 cm². 2 Selected detached pig skin with the stratum corneum facing upwards. The silk fibroin aqueous solution from Example 1 was squeezed into a dried microcurrent medical dressing and applied to the surface of the pig skin for simulated use for 30 minutes. The silk fibroin content in the pig skin was measured after 24 hours (BCA method).

[0049] Results: During the experiment, the microcurrent intensity measured by the microammeter was 80-150 μA, and the silk fibroin retention in the skin was 1.28 mg / cm³. 2 The results showed that the silk fibroin solution-activated self-generating permeation-enhancing medical dressing of the present invention can effectively generate microcurrents and significantly promote the skin penetration and retention of silk fibroin.

[0050] Example 2

[0051] I. Material Preparation

[0052] Conductive pastes: silver conductive paste (containing 50% silver powder, 8% conductive carbon powder, 40% acrylic polymer binder, and 2% propylene glycol solubilizer); zinc conductive paste (containing 45% zinc powder, 8% conductive carbon powder, 45% polyurethane binder, and 2% propylene glycol solubilizer).

[0053] Silk fibroin aqueous solution: Dissolve silk fibroin in purified water to prepare a solution with a mass percentage concentration of 1.0%, adjust the pH to 7.0 with 0.1mol / L NaOH, filter and sterilize with a 0.22μm filter membrane, and fill into aluminum foil bags.

[0054] Base fabric: silk fabric.

[0055] II. Preparation of Microcurrent Medical Adhesives

[0056] Preparation of microcurrent medical dressings: Symmetrical silver electrode areas (positive electrode) and zinc electrode areas (negative electrode) were printed on silk fabric using screen printing, with a 1.5 cm insulating gap between the two electrode areas. The dressings were then dried at 100℃ for 10 minutes to cure the electrodes. The electrode pattern consisted of grid-like lines and covered the forehead, cheeks, and chin areas.

[0057] Packaging: The prepared dried microcurrent medical dressing and the silk fibroin aqueous solution are sealed in separate compartments in the same outer packaging.

[0058] III. Effect Test

[0059] The same Franz diffusion cell in vitro transdermal experimental method as in Example 1 was used, and the experimental conditions were the same as in Example 1.

[0060] Results: During the experiment, the microcurrent intensity measured by a microammeter was 85-125 μA, and the skin retention of silk fibroin was 1.18 mg / cm², approximately 92% of that in Example 1 (1.28 mg / cm²). The results indicate that at a silk fibroin concentration of 1.0%, the silk fibroin solution-activated self-generating permeation-enhancing medical dressing of the present invention can still effectively generate microcurrents and significantly promote skin penetration and retention of silk fibroin, although the effect is slightly lower than that of Example 1 (2.0% concentration).

[0061] Example 3

[0062] I. Material Preparation

[0063] Conductive pastes: silver conductive paste (containing 50% silver powder, 5% conductive carbon powder, 43% silicone binder, and 2% propylene glycol solubilizer); zinc conductive paste (containing 45% zinc powder, 5% conductive carbon powder, 48% silicone binder, and 2% propylene glycol solubilizer).

[0064] Silk fibroin aqueous solution: Dissolve silk fibroin in purified water to prepare a solution with a mass percentage concentration of 0.5%, adjust the pH to 5.5 with 0.1 mol / L NaOH, filter and sterilize with a 0.22 μm filter membrane, and fill into aluminum foil bags.

[0065] Base fabric layer: knitted fabric.

[0066] II. Preparation Method

[0067] Preparation of microcurrent medical dressings: Symmetrical silver electrode areas (positive electrode) and zinc electrode areas (negative electrode) were printed on a knitted fabric using screen printing, with a 1.5 cm insulating gap between the two electrode areas. The dressings were then dried at 100℃ for 10 minutes to cure the electrodes. The electrode pattern consisted of stripes and covered the forehead, cheeks, and chin areas.

[0068] Packaging: The prepared dried microcurrent medical dressing and the silk fibroin aqueous solution are sealed in separate compartments in the same outer packaging.

[0069] III. Effect Test

[0070] The same Franz diffusion cell in vitro transdermal experimental method as in Example 1 was used, and the experimental conditions were the same as in Example 1.

[0071] Results: During the experiment, the microcurrent intensity measured by a microammeter was 50-80 μA, and the skin retention of silk fibroin was 1.05 mg / cm², approximately 82% of that in Example 1 (1.28 mg / cm²). The results indicate that at a silk fibroin concentration of 0.5%, the silk fibroin solution-activated self-generating permeation-enhancing medical dressing of the present invention can still generate microcurrents and promote skin penetration of silk fibroin; however, the microcurrent intensity and permeation-enhancing effect decrease with decreasing silk fibroin concentration.

[0072] Comparative Example 1 (No Electrode)

[0073] The electrolyte solution used was the same silk fibroin aqueous solution as in Example 1, and the base fabric layer was ordinary nonwoven fabric (without electrodes).

[0074] Results: The amount retained in the skin was 0.24 mg / cm³. 2 This is only for Example 1 (1.28 mg / cm³). 2 18.8% of ).

[0075] Comparative Example 2 (Different Electrolyte Solutions)

[0076] The same silver-zinc electrode nonwoven fabric as in Example 1 was used, but the electrolyte solution was purified water (without silk fibroin).

[0077] Results: During the experiment, the microcurrent intensity measured by the microammeter was less than 10 μA (deionized water is almost non-conductive). Since the electrolyte solution did not contain silk fibroin, the skin retention was 0 mg / cm³. 2 This comparative example shows that the aqueous solution of silk fibroin not only provides the active ingredients, but also serves as an electrolyte, which is a necessary condition for activating microcurrents; deionized water alone cannot generate effective microcurrents, nor can it achieve transdermal delivery of silk fibroin.

[0078] Data Analysis

[0079] Table 1 Summary of results on silk fibroin retention in the skin Group Silk fibroin concentration Electrode materials substrate Microcurrent (μA) <![CDATA[Skin retention amount (mg / cm 2 )]]> Relative value of skin retention Example 1 2% Ag / Zn nonwoven fabric 80-150 1.28 5.3 Example 2 1% Ag / Zn Silk cloth 85-125 1.18 4.9 Example 3 0.5% Ag / Zn Knitted fabric 50-80 1.05 4.4 Comparative Example 1 2% none nonwoven fabric 0 0.24 1.0 Comparative Example 2 0% (purified water) Ag / Zn nonwoven fabric ＜10 0 0

[0080] Performance testing

[0081] In vitro cell proliferation assay (MTT method)

[0082] The viable cells were prepared into a single-cell suspension and their numbers were counted to achieve a cell density of 1×10⁻⁶. 4 Cells were seeded at a density of 1 / mL in 96-well plates, with 200 μL of cell suspension added to each well. The plates were divided into six groups: control, Example 1, Example 2, Example 3, and Comparative Examples 1 and 2. After cell adhesion, the old culture medium was removed, and new culture medium containing the sample (sample:medium = 1:4) was added. The control group continued to use the same culture medium. Five replicates were set for each sample from Examples 1-3 and Comparative Examples 1-2. The plates were incubated at 37°C with 5% CO2 for 1, 2, and 3 days. At each time point, 20 μL of MTT solution (prepared with PBS at pH 7.4) was added to the corresponding well, and the plates were incubated for another 4 hours. The plates were then quickly inverted, the supernatant was discarded, and 150 μL of DMSO was added to each well. The plates were shaken for 10 min to dissolve the MTT crystals. The absorbance (OD) of each well at 490 nm was measured using an ELISA reader. 490 The absorbance was measured (value), and a cell-free well was set up as a control group for zeroing. The average value of 6 wells was taken. A bar chart of cell growth was plotted with culture time and absorbance as the coordinate axis, as shown below. Figure 2 As shown in the figure, the absorbance of the embodiments is higher than that of the comparative and control groups, indicating that the silk fibroin solution-activated self-generating permeation medical dressing prepared in the embodiments can significantly promote cell growth, thereby promoting wound repair.

[0083] Wound healing test

[0084] Fifty experimental rats were used, and their backs were scratched to create a wound model with an area of ​​approximately 1×2 cm. The rats were randomly divided into 5 groups of 10 each. The medical dressings of Examples 1-3 and Comparative Examples 1-2 were used to treat the wounds of each group of rats. The dressings were applied once a day for 30 minutes each time. The wound healing status of the rats was recorded after 2, 7, and 11 days of treatment. The wound size of each rat was measured, and the total wound area of ​​each group of rats was recorded. The wound healing rate of each group of rats after 2, 7, and 11 days of treatment was calculated. The specific results are shown in Table 2. Wound healing rate % = (wound area before treatment - wound area after treatment) / wound area before treatment × 100%.

[0085] Table 2 Wound healing rate Grouping Healing rate after 2 days (%) Healing rate after 7 days (%) Healing rate (%) after 11 days Example 1 12.5±2.0 56.7±3.2 95.1±2.4 Example 2 11.7±1.7 54.1±2.5 91.2±2.1 Example 3 11.2±1.9 51.1±3.5 88.2±1.9 Comparative Example 1 8.8±1.5 43.3±3.3 73.6±2.1 Comparative Example 2 6.6±2.1 36.2±2.6 66.5±2.8

[0086] Data Analysis

[0087] As shown in Table 2, the wound healing rate of Examples 1-3 was significantly higher than that of Comparative Examples 1-2, and Example 1 (2% silk fibroin + microcurrent) had the best effect, indicating that silk fibroin and microcurrent have a synergistic effect in promoting wound repair.

[0088] The above description is merely a preferred embodiment of the present invention and does not constitute any limitation on the present invention. Any simple modifications, alterations, or equivalent structural changes made to the above embodiments based on the technical essence of the invention shall still fall within the protection scope of the present invention.

Claims

1. A silk fibroin solution activated self-powered electro-osmotic medical patch, characterized in that, include: Base fabric layer; Microcurrent generating unit disposed on the base fabric layer; And an aqueous solution of silk fibroin packaged independently of the base fabric layer; The microcurrent generating unit includes a first metal electrode and a second metal electrode, the first metal electrode and the second metal electrode having different electrochemical potentials; The silk fibroin aqueous solution has a mass percentage concentration of 0.5%-2.0% and a pH of 5.5-7.

5. The silk fibroin aqueous solution serves as both the active ingredient to be absorbed transdermally and the electrolyte solution to activate the microcurrent generating unit. The first metal electrode material is silver or a silver-containing alloy, and the second metal electrode material is zinc or a zinc-containing alloy; The distance between the first metal electrode and the second metal electrode is 1.0-2.0 cm; When the silk fibroin aqueous solution is used and simultaneously comes into contact with the first metal electrode, the second metal electrode, and human skin, the first metal electrode, the second metal electrode, the silk fibroin aqueous solution, and human skin together form a closed galvanic cell circuit, spontaneously generating a microcurrent of 50-200 microamps. The microcurrent promotes the transdermal absorption of the silk fibroin itself through electroporation and iontophoresis effects.

2. The silk fibroin solution activated self-powered electro-osmotic medical patch according to claim 1, characterized in that, The base fabric layer is one of nonwoven fabric, silk fabric or knitted fabric. 3.The silk fibroin solution activated self-powered electro-osmotic medical patch according to claim 1, wherein, The microcurrent generating units are arranged in mutually insulated radial, grid, or strip patterns on the base fabric layer.

4. The silk fibroin solution activated self-powered electro-osmotic medical patch according to any one of claims 1-3, characterized in that, The first metal electrode and the second metal electrode are respectively formed by drying and curing conductive slurry; the conductive slurry includes metal powder, conductive carbon powder, binder and solubilizer, wherein the metal powder of the first metal electrode is silver powder and the metal powder of the second metal electrode is zinc powder; the binder is selected from one or more of acrylic polymer, silicone or polyurethane; the binder accounts for 40%-65% of the total mass of the slurry; the solubilizer is propylene glycol.

5. A preparation method of a silk fibroin solution activated self-powered electro-enhanced medical patch, characterized in that, Includes the following steps: a) Preparation of silk fibroin aqueous solution: Dissolve silk fibroin in purified water to obtain a silk fibroin aqueous solution with a mass percentage concentration of 0.5%-2.0% and a pH of 5.5-7.5; b) Preparation of microcurrent medical dressing: A first metal electrode and a second metal electrode are formed on a base fabric layer by screen printing. The first metal electrode and the second metal electrode are formed by drying and curing a conductive paste. The conductive paste contains metal powder, conductive carbon powder, binder and solubilizer. The metal powder of the first metal electrode is silver powder and the metal powder of the second metal electrode is zinc powder. The binder is selected from one or more of acrylic polymer, silicone or polyurethane. The binder accounts for 40%-65% of the total mass of the paste. The solubilizer is propylene glycol. c) Separate the silk fibroin aqueous solution from step a) and the microcurrent medical dressing from step b) into separate compartments or bags.

6. The application of the silk fibroin solution-activated self-generating permeation-enhancing medical dressing according to any one of claims 1-4 in the preparation of medical dressings with repair functions.

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