Non-contact processing apparatus, and immune cell activation device and activation structure thereof

Abstract

The application provides a non-contact processing device, and an immune cell activation device and an activation structure thereof. The activation structure includes a plurality of nano pillars, each having an activation end. The activation ends of the plurality of nano pillars define a plurality of first steps and a plurality of second steps. Each first step is higher than an adjacent second step by a height difference of between 3 microns and 12 microns along a thickness direction. The activation structure can be driven to concentrate electric charges on the plurality of first steps and the plurality of second steps, so that at least one immune cell is attracted to move to at least one first step and / or at least one second step, and is activated by touching the corresponding first activation end and / or the corresponding second activation end. Thus, the height difference facilitates the separation and activation of the plurality of immune cells by the plurality of first steps and the plurality of second steps.

Description

Technical Field

[0001] This application relates to a cell processing device, and more particularly to a non-contact processing device, an immune cell activation device, and its activation structure. Background Technology

[0002] While existing cell processing devices are equipped with non-contact cell sorting mechanisms, these mechanisms do not provide relevant activation structures for immune cells. Therefore, the applicant believes that these deficiencies can be improved and proposes this application with a rationally designed solution that effectively addresses these deficiencies. Summary of the Invention

[0003] The technical problem to be solved by this application is to provide a non-contact processing device and an immune cell activation device and its activation structure, which can effectively improve the defects that may occur in existing cell processing devices.

[0004] This application discloses a non-contact processing device, comprising: an immune cell activation device for containing a liquid sample having multiple immune cells; the immune cell activation device includes: an activation structure including a first substrate, a first electrode layer formed on the first substrate, and a group of nanopillars; wherein the group of nanopillars is located inside the activation structure and is distributed in a stepped manner, and the group of nanopillars includes: multiple first pillar groups, staggered from each other and each including multiple first nanopillars; each first nanopillar has a first activation end, and the first activation end of the multiple first nanopillars in each first pillar group defines a first step surface; multiple second pillar groups, staggered from each other and each including multiple second nanopillars; each second nanopillar has a second activation end, each The second activation end of the plurality of second nanopillars in the second column group defines a second step surface; wherein each second step surface is higher than the adjacent first step surface by a discontinuity along a thickness direction, which is smaller than the outer diameter of any immune cell; a mating structure is spaced apart from the activation structure along the thickness direction; wherein the mating structure includes a second substrate and a second electrode layer formed on the second substrate; a power supply device is electrically coupled to the first electrode layer and the second electrode layer; wherein the power supply device can drive the activation structure to concentrate a plurality of charges on a plurality of first step surfaces and a plurality of second step surfaces, so that each immune cell is attracted to move to at least one first step surface and / or at least one second step surface and is activated by contacting the corresponding first activation end and / or the corresponding second activation end.

[0005] Optionally, any two adjacent first nanopillars in each first column group are separated by a first distance between 0.1 micrometers and 1 micrometer, and any two adjacent second nanopillars in each second column group are separated by a second distance between 0.1 micrometers and 1 micrometer.

[0006] Optionally, each first activated end has a plurality of first protrusions, and each second activated end has a plurality of second protrusions.

[0007] Optionally, the plurality of first nanopillars in each first column group are arranged in a first matrix, forming M rows along a first direction perpendicular to the thickness direction and N columns along a second direction perpendicular to the thickness direction and the first direction; and M and N are both positive integers greater than 1.

[0008] Optionally, in two first pillar groups that are adjacent to each other in the first direction, a portion of a plurality of first nanopillars in one first pillar group is connected to a portion of a plurality of first nanopillars in the other first pillar group and arranged in L columns, where L is a positive integer less than N.

[0009] Optionally, among the three first pillar groups that are adjacent to each other in the first direction, a portion of the plurality of first nanopillars in the central first pillar group is arranged in column L1, and the remaining portion of the plurality of first nanopillars in the central first pillar group is arranged in column L2, with the portion of the plurality of first nanopillars in the other first pillar group being arranged in column L2; wherein L1 and L2 are each positive integers less than N, and the sum of L1 and L2 is equal to N.

[0010] Optionally, the plurality of second nanopillars in each second column group are arranged in a second matrix, with M rows along the first direction and N columns along the second direction.

[0011] Optionally, in two second pillar groups that are adjacent to each other in the first direction, a portion of a plurality of second nanopillars in one second pillar group is arranged in L columns, following a portion of a plurality of second nanopillars in the other second pillar group, and L is a positive integer less than N.

[0012] Optionally, the nanopillar group includes multiple third pillar groups, which are staggered and each includes multiple third nanopillars; each third nanopillar has a third activation end, and the third activation end of the multiple third nanopillars in each third pillar group defines a third-order surface; wherein each third-order surface is higher than the adjacent second-order surface by a discontinuity along the thickness direction, which is between 3 micrometers and 12 micrometers.

[0013] Optionally, the activated structure has a thickness between 600 micrometers and 750 micrometers, and a thickness difference between 3 micrometers and 12 micrometers.

[0014] Optionally, the activated structure includes a photoelectric layer located between the first substrate and the nanopillar group; wherein the photoelectric layer can be used to receive an external ambient light so that multiple charges are concentrated on multiple first-level surfaces and multiple second-level surfaces.

[0015] Optionally, the activation structure includes a photoelectric layer located between the first substrate and the nanopillar group; wherein, the non-contact processing device further includes: a light driving device facing the immune cell activation device; wherein, the light driving device can irradiate a light towards the activation structure to form a mesophoretic pattern through the photoelectric layer that can move any immune cell.

[0016] Optionally, the activated structure includes a photoelectric layer located between the first substrate and the nanopillar group; wherein the photoelectric layer can be used to receive light so that multiple charges are concentrated on multiple first-level surfaces and multiple second-level surfaces.

[0017] This application also discloses an immune cell activation device for containing a liquid sample containing multiple immune cells. The immune cell activation device includes: an activation structure comprising a first substrate, a first electrode layer formed on the first substrate, and a group of nanopillars; wherein the nanopillars are located inside the activation structure and are distributed in a stepped manner, and the nanopillars include: multiple first pillar groups, staggered from each other and each including multiple first nanopillars; each first nanopillar has a first activation end, and the first activation end of the multiple first nanopillars in each first pillar group defines a first stepped surface; multiple second pillar groups, staggered from each other and each including multiple second nanopillars; each second nanopillar has a second activation end, and the second activation end of the multiple second nanopillars in each second pillar group defines a second stepped surface; wherein each second stepped surface is higher than the adjacent first stepped surface by a discontinuity along a thickness direction, which is smaller than the outer diameter of any immune cell; and a mating structure spaced apart from the activation structure along the thickness direction; wherein the mating structure includes a second substrate and a second electrode layer formed on the second substrate. The activation structure can be driven to concentrate multiple charges on multiple first-level surfaces and multiple second-level surfaces, so that each immune cell is attracted to move to at least one first-level surface and / or at least one second-level surface and is activated by touching the corresponding first activation end and / or the corresponding second activation end.

[0018] Optionally, any two adjacent first nanopillars in each first column group are separated by a first distance between 0.1 micrometers and 1 micrometer, and any two adjacent second nanopillars in each second column group are separated by a second distance between 0.1 micrometers and 1 micrometer.

[0019] Optionally, each first activated end has a plurality of first protrusions, and each second activated end has a plurality of second protrusions.

[0020] Optionally, the plurality of first nanopillars in each first pillar group are arranged in a first matrix, forming M rows along a first direction perpendicular to the thickness direction and N columns along a second direction perpendicular to the thickness direction and the first direction; wherein, the plurality of second nanopillars in each second pillar group are arranged in a second matrix, forming M rows along the first direction and N columns along the second direction; wherein M and N are both positive integers greater than 1.

[0021] Optionally, in two first pillar groups that are adjacent to each other in the first direction, a portion of a plurality of first nanopillars in one first pillar group is connected to a portion of a plurality of first nanopillars in the other first pillar group and arranged in L columns, where L is a positive integer less than N.

[0022] Optionally, the activated structure has a thickness between 600 micrometers and 750 micrometers, and a thickness difference between 3 micrometers and 12 micrometers.

[0023] This application also discloses an activation structure for an immune cell activation device, comprising: a first substrate, a first electrode layer formed on the first substrate, and a group of nanopillars; wherein the group of nanopillars is located inside the activation structure and is distributed in a stepped manner, and the group of nanopillars includes: a plurality of first pillar groups, staggered from each other and each including a plurality of first nanopillars; each first nanopillar has a first activation end, and the first activation end of the plurality of first nanopillars in each first pillar group defines a first step surface; a plurality of second pillar groups, staggered from each other and each including a plurality of second nanopillars; each second nanopillar has a second activation end, and the second activation end of the plurality of second nanopillars in each second pillar group defines a second step surface; wherein each first step surface is higher than the adjacent second step surface by a discontinuity along a thickness direction, which is between 3 micrometers and 12 micrometers. The activation structure can be driven to concentrate multiple charges on multiple first-step surfaces and multiple second-step surfaces, so that at least one immune cell is attracted to move to at least one first-step surface and / or at least one second-step surface and is activated by touching the corresponding first activation end and / or the corresponding second activation end.

[0024] In summary, the non-contact processing device and immune cell activation apparatus and their activation structure disclosed in the embodiments of this application, through the discontinuity formed between each first step surface and the adjacent second step surface, facilitate the separation of multiple immune cells by multiple first step surfaces and multiple second step surfaces, thereby effectively activating each immune cell through multiple first activation ends and multiple second activation ends.

[0025] The other effects and embodiments of this application are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0026] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0027] Figure 1 This is a three-dimensional schematic diagram of a non-contact processing device according to Embodiment 1 of this application.

[0028] Figure 2 for Figure 1 A three-dimensional sectional view.

[0029] Figure 3 for Figure 1 A schematic cross-sectional view along section line III-III.

[0030] Figure 4 for Figure 1 A schematic cross-sectional view along section line IV-IV.

[0031] Figure 5 for Figure 4 A magnified diagram of region V.

[0032] Figure 6 for Figure 5 A schematic diagram of the first type of change.

[0033] Figure 7 for Figure 5 A diagram illustrating the second type of variation.

[0034] Figure 8 for Figure 5 A schematic diagram of the third type of variation.

[0035] Figure 9 for Figure 3 The following is a schematic diagram of the subsequent operation.

[0036] Figure 10 for Figure 4 The following is a schematic diagram of the subsequent operation.

[0037] Figure 11 This is a cross-sectional schematic diagram of the non-contact processing device according to Embodiment 2 of this application.

[0038] Figure 12 for Figure 11 The following is a schematic diagram of the subsequent operation. Detailed Implementation

[0039] The following specific embodiments illustrate the implementation of the "non-contact processing device and immune cell activation apparatus and its activation structure" disclosed in this application. Those skilled in the art can understand the advantages and effects of this application from the content disclosed in this specification. This application can be implemented or applied through other different specific embodiments, and various details in this specification can also be modified and changed based on different viewpoints and applications without departing from the concept of this application. Furthermore, the accompanying drawings are for simple illustration only and are not depictions of actual dimensions, as stated in advance. The following embodiments will further describe the relevant technical content of this application in detail, but the disclosed content is not intended to limit the scope of protection of this application.

[0040] It should be understood that while terms such as "first," "second," and "third" may be used in this document to describe various components or features, these components or features should not be limited by these terms. These terms are primarily used to distinguish one component from another, or one feature from another. Furthermore, the term "or" as used in this document should, as appropriate, include any combination of one or more related listed items.

[0041] [Example 1] Please see Figures 1 to 10 The image shows an embodiment of this application. This embodiment discloses a non-contact processing device 1000, which includes an immune cell activation device 100, a power supply device 200 electrically coupled to the immune cell activation device 100, and a light driving device 300 located corresponding to the immune cell activation device 100. However, this application is not limited to this. For example, in other embodiments not shown in this application, the non-contact processing device 1000 may selectively omit the light driving device 300; or, the immune cell activation device 100 may be used alone (e.g., for sale) or in combination with other components, depending on actual needs.

[0042] In this embodiment, the immune cell activation device 100 is a rectangular structure with chip-scale dimensions, and the immune cell activation device 100 is used to contain a liquid sample S having multiple immune cells P; that is, any device not applied to immune cells is different from the immune cell activation device 100 referred to in this embodiment.

[0043] The immune cell activation device 100 includes an activation structure 1, a mating structure 2 spaced apart from the activation structure 1 along a thickness direction H, and an adhesive layer 3 bonding the periphery of the activation structure 1 and the periphery of the mating structure 2. At least one of the activation structure 1 and the mating structure 2 is transparent, and in this embodiment, the activation structure 1 and the mating structure 2 are two plate-like structures arranged parallel to each other, with a distance between them greater than the size of any one of the immune cells P; however, this application is not limited to the above description.

[0044] More specifically, in this embodiment, the activation structure 1 has a thickness between 600 micrometers and 750 micrometers, and the activation structure 1 includes a first substrate 11, a first electrode layer 12 formed on the first substrate 11, a photoelectric layer 13 formed on the first substrate 11, and a group of nanopillars 14 formed on the photoelectric layer 13 (that is, the photoelectric layer 13 is located between the first substrate 11 and the group of nanopillars 14), but this application is not limited thereto. For example, in other embodiments not shown in this application, the photoelectric layer 13 of the activation structure 1 may be omitted or replaced with other structures according to actual needs; furthermore, the activation structure 1 may be used alone or in combination with other components.

[0045] In this embodiment, the first electrode layer 12 is formed on the bottom side of the first substrate 11, and the photoelectric layer 13 is formed on the top side of the first substrate 11. The photoelectric layer 13 has a plurality of transistors 131 arranged in a matrix. The photoelectric layer 13 can employ an NPN transistor architecture, a PNP transistor architecture, an NP diode architecture, or a PN diode architecture, depending on actual needs, but this application is not limited to these. That is, according to actual needs, the photoelectric layer 13 (e.g., the plurality of transistors 131) provided in this embodiment can be driven to provide corona discharge function for the immune cells P.

[0046] The mating structure 2 includes a second substrate 21 and a second electrode layer 22 formed on the second substrate 21, with the second electrode layer 22 facing the activated structure 1 (e.g., the photoelectric layer 13 and the nanopillar group 14). In this embodiment, the power supply device 200 (e.g., an AC power device) is electrically coupled to the first electrode layer 12 of the activated structure 1 and the second electrode layer 22 of the mating structure 2, so that the light driving device 300 can irradiate a light towards the activated structure 1 to form a dielectrophoretic pattern (not shown in the figure) through the photoelectric layer 13 that can move any one of the immune cells P.

[0047] For example, the light-driven device 300 may include a camera 301 and a light source 302 associated with the camera 301. The light-driven device 300 can emit light from the light source 302 to illuminate the activated structure 1, causing the activated structure 1 (or the photoelectric layer 13) to form the dielectrophoretic pattern, thereby moving any one of the immune cells P within the liquid sample S through the dielectrophoretic pattern.

[0048] To facilitate understanding of this embodiment, the above content first introduces the general cooperation relationship between the immune cell activation device 100, the power supply device 200, and the light driving device 300. The following content then describes the nanopillar group 14 of the activation structure 1.

[0049] The nanopillar group 14 is located inside the activated structure 1 and is distributed in a stepped manner. The nanopillar group 14 includes a plurality of first pillar groups 141 and a plurality of second pillar groups 142 that are staggered relative to each other. The plurality of first pillar groups 141 are also staggered relative to the plurality of second pillar groups 142.

[0050] Each of the first column groups 141 includes a plurality of first nanopillars 1411, and the first outer diameter R1 of each of the first column groups 141 is generally between 4 micrometers (μm) and 6 micrometers, and any two adjacent first nanopillars 1411 of each of the first column groups 141 are separated by a first distance G1 between 0.1 micrometers and 1 micrometer; the first distance G1 is preferably between 0.2 micrometers and 0.5 micrometers, but this application is not limited thereto.

[0051] In this embodiment, the plurality of first pillar groups 141 are configured substantially identically, and the plurality of first nanopillars 1411 in each first pillar group 141 have the same structure and are arranged in a first matrix. However, this application is not limited thereto. For example, in other embodiments not shown in this application, the plurality of first pillar groups 141 may adopt different configurations; or, the plurality of first nanopillars 1411 in each first pillar group 141 may have slightly different structures or be arranged in a non-matrix manner (e.g., a polygonal arrangement with at least five sides).

[0052] Furthermore, in this embodiment, the first matrix arrangement is arranged in M ​​rows along a first direction D1 perpendicular to the thickness direction H, and in N columns along a second direction D2 perpendicular to both the thickness direction H and the first direction D1. Here, M and N are both positive integers greater than 1. In this embodiment, M is equivalent to N and can be between 2 and 10, and M and N are illustrated as 3 in the accompanying drawings, but this is not a limitation.

[0053] From another perspective, in two adjacent first column groups 141 in the first direction D1, a portion of the plurality of first nanopillars 1411 in one first column group 141 is connected to a portion of the plurality of first nanopillars 1411 in the other first column group 141, arranged in an L-column arrangement. Here, L is a positive integer less than N (e.g., L in this embodiment is 1 or 2).

[0054] Furthermore, among the three first column groups 141 that are adjacent to each other in the first direction D1, a portion of the first nanopillars 1411 of the central first column group 141 is arranged in column L1, and the remaining portion of the first nanopillars 1411 of the central first column group 141 is arranged in column L2, with the portion of the first nanopillars 1411 of the other first column group 141 located on its other side. Here, L1 and L2 are each positive integers less than N, and the sum of L1 and L2 is equal to N (e.g., L1 and L2 are 1 and 2 respectively in this embodiment).

[0055] Furthermore, each of the first nanopillars 1411 has a first activation end 1412, and the first activation ends 1412 of the plurality of first nanopillars 1411 in each first pillar group 141 define a first step surface 1414. It should be noted that each first activation end 1412 can be formed into a specific shape according to actual needs (e.g., ...). Figure 6 The cone shape shown Figure 7 The pyramid shape shown, or Figure 8 The plurality of first protrusions 1413 shown, and each of the first activated ends 1412 may also be attached with an activating substance, thereby effectively enhancing the activation effect of the immune cells P.

[0056] Each second column group 142 includes a plurality of second nanopillars 1421, and the second outer diameter R2 of each second column group 142 is generally between 4 micrometers (μm) and 6 micrometers. In this embodiment, the second outer diameter R2 is generally equivalent to the first outer diameter R1. Each second column group 142 is separated from any two adjacent second nanopillars 1421 by a second distance G2 between 0.1 micrometers and 1 micrometer, and the second distance G2 is preferably between 0.2 micrometers and 0.5 micrometers, but this application is not limited thereto.

[0057] In this embodiment, the plurality of second pillar groups 142 are configured substantially identically, and the plurality of second nanopillars 1421 in each second pillar group 142 have the same structure and are arranged in a second matrix, but this application is not limited thereto. For example, in other embodiments not shown in this application, the plurality of second pillar groups 142 may adopt different configurations; or, the plurality of second nanopillars 1421 in each second pillar group 142 may have slightly different structures or be arranged in a non-matrix manner (e.g., a polygonal arrangement with at least five sides).

[0058] Furthermore, in this embodiment, the second matrix arrangement is arranged in M ​​rows along the first direction D1 and in N columns along the second direction D2; that is, the second matrix arrangement in this embodiment is described in a configuration that is generally similar to the first matrix arrangement, but this application is not limited thereto. For example, in other embodiments not shown in this application, the second matrix arrangement may also adopt a configuration different from the first matrix arrangement according to actual needs.

[0059] From another perspective, in two adjacent second column groups 142 in the first direction D1, a portion of the plurality of second nanopillars 1421 in one second column group 142 is connected to a portion of the plurality of second nanopillars 1421 in the other second column group 142 in an L-column arrangement. Here, L is a positive integer less than N (e.g., L is 1 or 2 in this embodiment).

[0060] Furthermore, among the three second column groups 142 that are adjacent to each other in the first direction D1, a portion of the plurality of second nanopillars 1421 of the second column group 142 located in the center is arranged in column L1, and the remaining portion of the plurality of second nanopillars 1421 of the second column group 142 located in the center is arranged in column L2, and a portion of the plurality of second nanopillars 1421 of the second column group 142 located on the other side is arranged in column L2; wherein, L1 and L2 are each positive integers less than N, and the sum of L1 and L2 is equal to N (e.g., L1 and L2 are 1 and 2 respectively in this embodiment).

[0061] Furthermore, each of the second nanopillars 1421 has a second activation end 1422, and the second activation ends 1422 of the plurality of second nanopillars 1421 in each second pillar group 142 define a second step surface 1424. It should be noted that each second activation end 1422 can be formed into a specific shape according to actual needs (e.g., ...). Figure 6 The cone shape shown Figure 7 The pyramid shape shown, or Figure 8The plurality of second protrusions 1423 shown, and each of the second activated ends 1422 may also be attached with an activating substance, thereby effectively enhancing the activation effect of the immune cells P.

[0062] The plurality of first-step surfaces 1414 are staggered relative to the plurality of second-step surfaces 1424, and the outer contour of any one of the plurality of first-step surfaces 1414 and the plurality of second-step surfaces 1424 may be slightly larger than the size of the immune cell P, thereby making it easier to separate the plurality of immune cells P, but not limited thereto.

[0063] Furthermore, each of the first surface 1414 is higher than the adjacent second surface 1424 along the thickness direction H by a discontinuity S1, which is smaller than the outer diameter of any of the immune cells P. In other words, the discontinuity S1 is between 3 micrometers and 12 micrometers, and preferably between 6 micrometers and 8 micrometers, but this application is not limited to this.

[0064] As described above, the power supply device 200 can drive the activation structure 1 to concentrate multiple charges on multiple first step surfaces 1414 and multiple second step surfaces 1424, so that each immune cell P is attracted to move to at least one first step surface 1414 and / or at least one second step surface 1424 and is activated by touching the corresponding first activation end 1412 and / or the corresponding second activation end 1422.

[0065] Therefore, in this embodiment, the non-contact processing device 1000 can facilitate the separation of multiple immune cells P by the discontinuity S1 formed by each first step surface 1414 and the adjacent second step surface 1424 through the discontinuity S1, thereby effectively activating each immune cell P through the multiple first activation ends 1412 and the multiple second activation ends 1422.

[0066] Furthermore, the photoelectric layer 13 can also be used to generate multiple charges concentrated on multiple first-step surfaces 1414 and multiple second-step surfaces 1424 by receiving external ambient light; or, the photoelectric layer 13 can also generate multiple charges concentrated on multiple first-step surfaces 1414 and multiple second-step surfaces 1424 by emitting light from the light driving device 300. This application does not limit this.

[0067] [Example 2] Please see Figure 11 and Figure 12As shown, this is Embodiment Two of this application. Since this embodiment is similar to Embodiment One described above, the similarities between the two embodiments will not be repeated. The main differences between this embodiment and Embodiment One described above are explained as follows: In this embodiment, the nanopillar group 14 further includes a plurality of third pillar groups 143, and the plurality of third pillar groups 143 are staggered with each other and each includes a plurality of third nanopillars 1431. The plurality of third pillar groups 143 are staggered with respect to the plurality of first pillar groups 141 and the plurality of second pillar groups 142, and the structure of the third nanopillars 1431 is similar to that of the first nanopillars 1411 or the second nanopillars 1421, the only difference being that the height of the third nanopillars 1431 is greater than that of the first nanopillars 1411 and also higher than that of the second nanopillars 1421.

[0068] Furthermore, each of the third nanopillars 1431 has a third activation end 1432, and the third activation end 1432 of the plurality of third nanopillars 1431 in each third pillar group 143 defines a third step surface 1433. Each third step surface 1433 is higher than the adjacent second step surface 1424 along the thickness direction H by a discontinuity S2, which is between 3 micrometers and 12 micrometers (preferably between 6 micrometers and 8 micrometers).

[0069] [Technical Effects of the Embodiments in this Application] In summary, the non-contact processing device and immune cell activation apparatus and their activation structure disclosed in the embodiments of this application, through the discontinuity formed between each first step surface and the adjacent second step surface, facilitate the separation of multiple immune cells by multiple first step surfaces and multiple second step surfaces, thereby effectively activating each immune cell through multiple first activation ends and multiple second activation ends.

[0070] The embodiments and / or implementation methods described above are merely preferred embodiments and / or implementation methods for implementing the technology of this application, and are not intended to limit the implementation methods of the technology of this application in any way. Any person skilled in the art may make some modifications or alterations to other equivalent embodiments without departing from the scope of the technical means disclosed in this application, but these should still be regarded as the technology or embodiments that are substantially the same as those of this application.

Claims

1. A non-contact treatment apparatus characterized by comprising: The non-contact processing equipment includes: An immune cell activation device for containing a liquid sample containing multiple immune cells; the immune cell activation device includes: An activation structure includes a first substrate, a first electrode layer formed on the first substrate, and a group of nanopillars; wherein the group of nanopillars is located inside the activation structure and is distributed in a stepped manner, and the group of nanopillars includes: Multiple first pillar groups are staggered and each includes multiple first nanopillars; each first nanopillar has a first activation end, and the first activation end of the multiple first nanopillars in each first pillar group defines a first step surface; and Multiple second pillar groups are staggered and each includes multiple second nanopillars; each second nanopillar has a second activation end, and the second activation end of the multiple second nanopillars in each second pillar group defines a second step surface; Wherein, each of the second-order surfaces is raised by a discontinuity along a thickness direction relative to the adjacent first-order surface, which is smaller than the outer diameter of any of the immune cells; and A mating structure, spaced apart from the activation structure along the thickness direction; wherein the mating structure includes a second substrate and a second electrode layer formed on the second substrate; and A power supply device is electrically coupled to the first electrode layer and the second electrode layer; wherein the power supply device can drive the activation structure to concentrate multiple charges on multiple first surfaces and multiple second surfaces, so that each immune cell is attracted to move to at least one first surface and / or at least one second surface and is activated by touching the corresponding first activation end and / or the corresponding second activation end.

2. The non-contact processing device according to claim 1, characterized in that, Each of the first column groups is separated by a first distance between any two adjacent first nanocolumns, which is between 0.1 micrometers and 1 micrometer, and each of the second column groups is separated by a second distance between any two adjacent second nanocolumns, which is between 0.1 micrometers and 1 micrometer.

3. The non-contact processing device according to claim 1, characterized in that, Each of the first activated ends has a plurality of first protrusions, and each of the second activated ends has a plurality of second protrusions.

4. The non-contact processing device according to claim 1, characterized in that, The plurality of first nanopillars in each first column group are arranged in a first matrix, forming M rows along a first direction perpendicular to the thickness direction and N columns along a second direction perpendicular to both the thickness direction and the first direction; and M and N are both positive integers greater than 1.

5. The non-contact processing device according to claim 4, characterized in that, In two first column groups that are adjacent to each other in the first direction, portions of the first nanopillars in one first column group are arranged in L columns, where L is a positive integer less than N.

6. The non-contact processing device according to claim 4, characterized in that, In the three first column groups that are adjacent to each other in the first direction, a portion of the first nanopillars of the first column group located in the center is connected to a portion of the first nanopillars of the first column group located on one side of it and arranged in column L1, and the remaining portion of the first nanopillars of the first column group located in the center is connected to a portion of the first nanopillars of the first column group located on the other side of it and arranged in column L2; wherein L1 and L2 are each positive integers less than N, and the sum of L1 and L2 is equal to N.

7. The non-contact processing device according to claim 4, characterized in that, The plurality of second nanopillars in each second column group are arranged in a second matrix, forming M rows along the first direction and N columns along the second direction.

8. The non-contact processing device according to claim 7, characterized in that, In two second column groups that are adjacent to each other in the first direction, portions of a plurality of second nanopillars in one second column group are arranged in L columns, where L is a positive integer less than N.

9. The non-contact processing device according to claim 1, characterized in that, The nanopillar group includes multiple third pillar groups, which are staggered and each includes multiple third nanopillars; each third nanopillar has a third activation end, and the third activation end of the multiple third nanopillars in each third pillar group defines a third-order surface; wherein each third-order surface is higher than the adjacent second-order surface along the thickness direction by a discontinuity, which is between 3 micrometers and 12 micrometers.

10. The non-contact processing device according to claim 1, characterized in that, The activated structure has a thickness between 600 micrometers and 750 micrometers, and the discontinuity is between 3 micrometers and 12 micrometers.

11. The non-contact processing device according to claim 1, characterized in that, The activated structure includes a photoelectric layer located between the first substrate and the nanopillar group; wherein the photoelectric layer can be used to receive an external ambient light so that multiple charges are concentrated on multiple first-step surfaces and multiple second-step surfaces.

12. The non-contact processing device according to claim 1, characterized in that, The activation structure includes a photoelectric layer located between the first substrate and the nanopillar group; wherein, the non-contact processing device further includes: A light-driven device is provided facing the immune cell activation device; wherein the light-driven device can irradiate a light beam toward the activation structure to form a mesophoretic pattern through the photoelectric layer that allows any one of the immune cells to move.

13. The non-contact processing device according to claim 11, characterized in that, The activated structure includes a photoelectric layer located between the first substrate and the nanopillar group; wherein the photoelectric layer can be used to receive light so that multiple charges are concentrated on multiple first-step surfaces and multiple second-step surfaces.

14. An immune cell activation device, characterized in that, The immune cell activation device is used to contain a liquid sample having multiple immune cells, and the immune cell activation device includes: An activation structure includes a first substrate, a first electrode layer formed on the first substrate, and a group of nanopillars; wherein the group of nanopillars is located inside the activation structure and is distributed in a stepped manner, and the group of nanopillars includes: Multiple first pillar groups are staggered and each includes multiple first nanopillars; each first nanopillar has a first activation end, and the first activation end of the multiple first nanopillars in each first pillar group defines a first step surface; and Multiple second pillar groups are staggered and each includes multiple second nanopillars; each second nanopillar has a second activation end, and the second activation end of the multiple second nanopillars in each second pillar group defines a second step surface; Wherein, each of the second-order surfaces is raised by a discontinuity along a thickness direction relative to the adjacent first-order surface, the difference being smaller than the outer diameter of any of the immune cells; and A mating structure is spaced apart from the activation structure along the thickness direction; wherein the mating structure includes a second substrate and a second electrode layer formed on the second substrate; The activation structure can be driven to concentrate multiple charges on multiple first-step surfaces and multiple second-step surfaces, so that each immune cell is attracted to move to at least one first-step surface and / or at least one second-step surface and is activated by touching the corresponding first activation end and / or the corresponding second activation end.

15. The immune cell activation device according to claim 14, characterized in that, Each of the first column groups is separated by a first distance between any two adjacent first nanocolumns, which is between 0.1 micrometers and 1 micrometer, and each of the second column groups is separated by a second distance between any two adjacent second nanocolumns, which is between 0.1 micrometers and 1 micrometer.

16. The immune cell activation device according to claim 14, characterized in that, Each of the first activated ends has a plurality of first protrusions, and each of the second activated ends has a plurality of second protrusions.

17. The immune cell activation device according to claim 14, characterized in that, The plurality of first nanopillars in each first column group are arranged in a first matrix, forming M rows along a first direction perpendicular to the thickness direction and N columns along a second direction perpendicular to both the thickness direction and the first direction; wherein, the plurality of second nanopillars in each second column group are arranged in a second matrix, forming M rows along the first direction and N columns along the second direction; wherein M and N are both positive integers greater than 1.

18. The immune cell activation device according to claim 17, characterized in that, In two first column groups that are adjacent to each other in the first direction, portions of the first nanopillars in one first column group are arranged in L columns, where L is a positive integer less than N.

19. The immune cell activation device according to claim 14, characterized in that, The activated structure has a thickness between 600 micrometers and 750 micrometers, and the discontinuity is between 3 micrometers and 12 micrometers.

20. An activation structure for an immune cell activation device, characterized in that, The activation structure of the immune cell activation device includes: a first substrate, a first electrode layer formed on the first substrate, and a group of nanopillars; wherein the group of nanopillars is located inside the activation structure and is distributed in a stepped manner, and the group of nanopillars includes: Multiple first pillar groups are staggered and each includes multiple first nanopillars; each first nanopillar has a first activation end, and the first activation end of the multiple first nanopillars in each first pillar group defines a first step surface; and Multiple second pillar groups are staggered and each includes multiple second nanopillars; each second nanopillar has a second activation end, and the second activation end of the multiple second nanopillars in each second pillar group defines a second step surface; Each of the first-step surfaces is raised by a discontinuity along a thickness direction relative to the adjacent second-step surface, which is between 3 micrometers and 12 micrometers. The activation structure can be driven to concentrate multiple charges on multiple first-step surfaces and multiple second-step surfaces, so that at least one immune cell is attracted to move to at least one first-step surface and / or at least one second-step surface and is activated by touching the corresponding first activation end and / or the corresponding second activation end.

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