A cell processing system based on an inverted cone centrifuge chamber and a control method thereof

Abstract

The application discloses a cell processing system based on an inverted cone centrifugal cabin and a control method thereof, and is applied to a cell processing system comprising an inverted cone centrifugal cabin and a centrifugal motor. The cell processing system can execute liquid feeding, centrifugal separation, cleaning and extraction in a time sequence. In the liquid feeding stage, a mixed solution of fat matrix components and a solution is delivered into the centrifugal cabin. In the centrifugal separation stage, the centrifugal motor is controlled to rotate at a set separation rotating speed and maintain a set separation time length, so that the mixed solution is centrifugally separated and waste liquid is discharged. In the cleaning stage, cleaning liquid is delivered into the centrifugal cabin, waste liquid is discharged after centrifugal separation, and the cleaning stage is executed at least once. In the extraction stage, the centrifugal motor is controlled to stop rotating, the fat matrix components gather at the bottom of the cabin along the conical cabin wall under the action of gravity and are discharged to a collection container. The geometric characteristics of the inverted cone centrifugal cabin are matched with the physical characteristics of density stratification, so that the enrichment and extraction of the fat matrix components are automatically completed in a closed environment, and the recovery rate of target components is improved.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, specifically to a cell processing system based on an inverted conical centrifuge chamber and its control method for processing adipose matrix components. Background Technology

[0002] The application of adipose-derived regenerative cell populations in tissue repair and regenerative medicine is becoming increasingly widespread. Their preparation typically begins with adipose aspirate or enzymatically digested adipose tissue, requiring a series of processing steps including mixing, centrifugation, waste removal, multiple washings, and extraction of target components to obtain clinically usable adipose matrix components. As these cell products move towards standardization and large-scale production, the aseptic nature, closed system, and batch-to-batch consistency of the processing have become core constraints in process design: the entire process must be completed in a closed, sterile environment; any opening, dispensing, or manual transfer increases the risk of contamination and introduces batch-to-batch variations. Due to this constraint, material transfer between processing steps generally relies on pipelines and pumping mechanisms, rather than open operations.

[0003] Regarding centrifugation in a closed environment, existing technologies have provided various device-level improvements. For example, Chinese utility model patent (authorization announcement number CN218048431U) with patent number CN202222380515.1 discloses a centrifuge chamber communication device, which includes a hollow stationary part and a rotating part passing through the stationary part. The rotating part has a conveying channel communicating with the centrifuge chamber, and the outer surface of the stationary part is provided with through holes and annular grooves corresponding to the conveying channel, so that liquid can still enter and exit the centrifuge chamber through the through holes when it is rotating. For example, Chinese invention patent application CN202511306218.4 (publication number CN121042178A) discloses a centrifugation device. The insert shaft of its separator has a dispensing passage formed inside. Rotary shaft seals are spaced apart on the outer wall of the insert shaft, forming an annular dispensing chamber between adjacent seals. Liquid pumped by a peristaltic pump enters each centrifuge container through the supply pipe, the annular dispensing chamber, and the dispensing passage. A buffer section on the cover engages with the annular abutment wall via a buffer groove, preventing the dispensing shell from rotating with the centrifuge shaft during centrifugation and avoiding entanglement of the supply pipe. While the above structures solve the problem of liquid supply and drainage connectivity during rotation, they do not address the specific control methods for each step of centrifugation enrichment, washing, and extraction. Therefore, it is impossible to directly obtain standardized cell products using only such devices.

[0004] Regarding separation methods, existing solutions mostly employ a filter-based sieving approach. For example, Chinese invention patent CN201680071465.3 (authorization announcement CN108367302B) discloses a centrifuge and its centrifugal separation method. The centrifuge chamber has a vertical guide section and a storage section. The method involves first rotating the centrifuge chamber to allow the target component to pass through the filter and enter the storage section under centrifugal force. Then, the rotation speed is reduced to allow other components to return to the centrifuge chamber by gravity. Finally, the predetermined components are discharged through the outlet of the storage section. This document also describes a centrifuge chamber with a cross-section that gradually narrows from top to bottom, such as an inverted conical shape. Japanese patent application JP2010201196A (publication JP2012055226A) discloses a cell processing device that uses a hydrophobic filter to divide a container into an upper concentration section and a lower digestion section. Cleaning and recovery are completed by spraying cleaning fluid into the concentration section using a jet nozzle and suction nozzles to draw up the supernatant. The separation effect of filtration-based methods relies on the filter as the core component. However, filter meshes are easily clogged by cells and tissue debris, and cleaning is time-consuming. Jet cleaning has limited effectiveness against cell particles adhering to the chamber wall. The deceleration, gravity return, and re-acceleration process also increases the processing cycle and disturbs the target components. The methods described in the aforementioned literature revolve around their respective filtration structures, and no feasible technical solution is provided for automating the transport, separation, multiple cleanings, and extraction within the same centrifuge container without relying on filters.

[0005] It is evident that the shape of the conical centrifuge chamber is already publicly known in existing technologies. However, the combination of the geometric characteristics of the conical chamber with the physical phenomena of centrifugal stratification and gravity convergence after shutdown provides a unique foundation for the implementation of a stratification, blocking, convergence, and extraction control method. This foundation has not yet been fully utilized in existing technologies. Current centrifugation processes still require switching pumps and valves between steps and manually determining the inlet endpoint, waste discharge timing, and cleaning endpoint, lacking a unified time-axis control logic. Therefore, a control method is needed that can fully utilize the geometric and physical characteristics of the inverted conical centrifuge chamber and automatically complete the entire process of inlet, centrifugal separation, cleaning, and extraction in a closed environment. Summary of the Invention

[0006] In order to overcome the problems of manual operation in the centrifugation process and the clogging and time-consuming cleaning of the filter separation route in the existing technology, the present invention provides a control method for a cell processing system based on an inverted conical centrifuge chamber.

[0007] To achieve the above objectives, the present invention adopts the following technical solution.

[0008] A control method for a cell processing system is disclosed, applied to a cell processing system including an inverted conical centrifuge chamber and a centrifuge motor driving the centrifuge chamber to rotate. The control method includes a liquid feeding stage, a centrifugation separation stage, a washing stage, and an extraction stage executed sequentially in time. In the liquid feeding stage, a mixture of fatty matrix components and a solution is delivered into the centrifuge chamber. In the centrifugation separation stage, the centrifuge motor is controlled to rotate at a set separation speed and maintain a set separation time, causing the fatty matrix components in the mixture to separate into layers with the waste liquid, and the waste liquid is discharged from the centrifuge chamber. In the washing stage, washing fluid is delivered into the centrifuge chamber, the centrifuge motor is controlled to rotate, causing the washing fluid to separate into layers with the fatty matrix components, and the waste liquid is discharged from the centrifuge chamber. The washing stage is performed at least once. In the extraction stage, the centrifuge motor is stopped, and the fatty matrix components, under the influence of gravity, converge along the conical wall of the centrifuge chamber to the bottom of the centrifuge chamber. The converged fatty matrix components are discharged into a collection container.

[0009] The control method described above uses an inverted conical centrifuge chamber as the controlled object, automatically connecting the enrichment, washing, and extraction of adipose matrix components into four stages along a time axis. During rotation, the densest adipose matrix components concentrate on both sides of the chamber. After stopping, the components automatically converge at the bottom of the chamber by gravity along the conical walls. Geometric characteristics and physical phenomena serve the separation and extraction stages respectively, eliminating the need for manual material transfer or intermediate separation elements such as filters between the two stages. The four stages are executed in a closed loop within the same centrifuge chamber in the sequence of liquid inlet, centrifugation, washing, and extraction, replacing the manual operation of multiple pumping, centrifugation, and waste discharge. This allows the entire process to be completed automatically in a closed environment, improving process consistency and the recovery rate of the target components.

[0010] In some embodiments of the present invention, the liquid inlet stage includes opening the liquid inlet channel valve and starting the liquid inlet pump to deliver the mixture into the centrifuge chamber at a set liquid inlet flow rate, and recording the running time of the liquid inlet pump to obtain the liquid inlet volume. This setup allows the liquid inlet process to be automatically completed by the sequential actions of the valve and pump, and the liquid inlet volume is obtained by multiplying the pump running time by the flow rate, providing a metering basis for calculating the waste discharge volume and cleaning fluid volume in subsequent stages, and the volume connection between each stage no longer relies on manual estimation.

[0011] In some embodiments of the present invention, the control method further includes detecting the state of the liquid in the inlet pipeline using an optical sensor. When the optical sensor detects that the state of the liquid changes from a mixture state to an oil state, the inlet pump is stopped and the inlet channel valve is closed. Since the mixture contains fatty matrix components and solution, its optical state differs detectably from that of pure oil. This design allows the inlet endpoint to be automatically determined based on the actual state of the liquid in the pipeline, and the inlet is promptly cut off at the moment the mixture delivery is completed, preventing excess oil from being pushed into the centrifuge chamber during the operation gap between the pump and the valve.

[0012] In some embodiments of the present invention, the control method further includes stopping the inlet pump and closing the inlet channel valve when the inlet volume reaches a set capacity threshold. The capacity threshold corresponds to the capacity of the centrifuge chamber. This setting provides an independent determination condition based on volume measurement as the inlet endpoint, which is redundant with the determination condition based on liquid state. Even if there are fluctuations in optical detection, the inlet volume will not exceed the upper limit of the centrifuge chamber's capacity.

[0013] In some embodiments of the present invention, the control method further includes monitoring the inlet pressure. When the inlet pressure exceeds a set pressure threshold and remains so for a set duration, the rotary seal structure or pipeline is determined to be blocked, triggering an alarm and shutting down the machine. The continuous increase in inlet pressure is a direct reflection of blockage in the rotary seal structure or pipeline. This design allows blockage to be identified and blocked before it causes pump overload or pipeline rupture, protecting the integrity of the closed environment.

[0014] In some embodiments of the present invention, during the centrifugal separation stage, discharging the waste liquid from the centrifuge chamber includes opening the drain channel valve and starting the drain pump at a set discharge time point while maintaining the separation speed, thereby discharging the waste liquid from the centrifuge chamber. This arrangement allows the discharge of waste liquid and centrifugal stratification to occur simultaneously. During discharge, the stratification state of the waste liquid and the fatty matrix components is always maintained by centrifugal force, eliminating the need to slow down and wait for the components to return before discharge. This shortens the processing cycle and avoids disturbance to the already stratified components caused by slowing down and then accelerating again.

[0015] In some embodiments of the present invention, a baffle is provided inside the centrifuge chamber to separate waste liquid from the fatty matrix components during centrifugation. A drain pump discharges the waste liquid at a set drain flow rate, which is set to prevent the fatty matrix components from being attracted by the suction of the drain pump and climbing over the baffle. The baffle provides a physical retention boundary for the fatty matrix components inside the centrifuge chamber. The drain flow rate is coordinated with the blocking capacity of the baffle: if the drain flow rate is too high, the suction generated by the drain pump will attract the fatty matrix components towards the drain outlet, causing them to climb over the baffle and be lost with the waste liquid; by setting the drain flow rate within the range that the baffle can block, the fatty matrix components are completely retained while the waste liquid is discharged, and the loss of the target components during the waste discharge process is avoided.

[0016] In some embodiments of the present invention, the running time of the drain pump is set according to the volume of the waste liquid to be drained and the drain flow rate. This setting ensures that the volume of waste liquid discharged each time corresponds to the actual volume of the waste liquid to be drained, avoiding both waste liquid residue in the tank due to insufficient waste discharge and unnecessary suction of the fatty matrix components due to excessively long waste discharge time. The waste discharge endpoint is determined by volume measurement, which is repeatable.

[0017] In some embodiments of the present invention, the control method further includes closed-loop control of the centrifugal motor speed to maintain speed fluctuations within a set range. The stability of the speed directly determines the stability of the centrifugal force field, which in turn determines the clarity of stratification and the stability of the enrichment location of fatty matrix components. This setup ensures that the separation process is unaffected by load changes and motor characteristic drift, maintaining consistent separation results between batches.

[0018] In some embodiments of the present invention, the control method further includes monitoring the vibration amplitude of the centrifugal motor, and controlling the centrifugal motor to stop when the vibration amplitude exceeds a set amplitude threshold. Exceeding the vibration amplitude limit is a sign of rotor dynamic imbalance, which, under high-speed rotation, may develop into equipment damage and disruption of the enclosed environment. This design ensures that dynamic imbalance is addressed by stopping the machine before reaching a dangerous level, protecting the safety of the equipment and the materials inside the chamber.

[0019] In some embodiments of the present invention, during the cleaning stage, the delivery of cleaning fluid into the centrifuge chamber includes activating an inlet pump to deliver the cleaning fluid into the centrifuge chamber at a set cleaning flow rate. The volume of the cleaning fluid is set according to the capacity of the centrifuge chamber and the volume of the fatty matrix components. This arrangement ensures that the cleaning fluid added each time can fill the space in the centrifuge chamber not occupied by the fatty matrix components, that the cleaning fluid is in full contact with the fatty matrix components, that the cleaning effect is stable, and that the amount of cleaning fluid used matches the volume of the chamber, preventing both overflow and under-filling.

[0020] In some embodiments of the present invention, the cleaning stage also includes a resuspension action, i.e., controlling the centrifuge motor to perform a centrifugation-stop-centrifugation cycle, so that the cells adhering to the walls and those settled at the bottom are resuspended. After centrifugation, some cells adhere to the chamber walls, while others settle at the bottom. If the cleaning solution is injected statically, the cells adhering to the walls and those settled at the bottom will have difficulty contacting the cleaning solution, resulting in insufficient cleaning. The centrifugation-stop-centrifugation cycle causes the liquid in the chamber to repeatedly experience alternating acceleration and stillness. Under the action of acceleration changes, the cells adhering to the walls and those settled at the bottom detach from their attachment positions and re-enter a suspended state. This setting allows the cleaning solution to contact all cells, improving the thoroughness of cleaning, and eliminating the need for additional flushing structures such as nozzles in the chamber.

[0021] In some embodiments of the present invention, the resuspension action includes increasing the speed of the centrifuge motor from zero to a set resuspension speed within a set time period, and then decreasing the speed from the resuspension speed to zero within a set time period. The resuspension action is repeated a set number of cycles. The time and speed of the raising and lowering process are limited to a range that allows cells to detach from their attachment without causing them to re-compact and accumulate. The number of cycles ensures that adherent and settled cells are detached batch by batch. This setup makes the resuspension effect quantifiable and repeatable, independent of the operator's experience and judgment.

[0022] In some embodiments of the present invention, the cleaning stage is repeated a predetermined number of times, each execution including the delivery of cleaning solution, centrifugation for separation, and discharge of waste liquid from the centrifuge chamber. This arrangement ensures that residual enzyme solution, free lipid droplets, and other impurities are progressively diluted and discharged with each cleaning. The number of cleaning cycles can be set according to the target purity requirements, and the cleaning depth and processing cycle can be balanced as needed.

[0023] In some embodiments of the present invention, during the extraction stage, discharging the accumulated fatty matrix components into the collection container includes opening the extraction channel valve and starting the extraction pump to discharge the fatty matrix components at a set extraction flow rate. The running time of the extraction pump is set according to the volume of the fatty matrix components and a set excess coefficient. After stopping, the fatty matrix components have accumulated along the conical chamber wall to the bottom of the chamber. The extraction pump only needs to extract at a low flow rate at the accumulation position. If the extraction flow rate is too high, it will disturb the accumulation layer and draw in residual waste liquid; if it is too low, it will prolong the processing time. The extraction flow rate is set at a level adapted to the stable state of the accumulation layer, and the running time is set according to the excess multiple of the volume of the fatty matrix components, so that the fatty matrix components accumulated at the bottom of the chamber, along with the residual portion in the extraction channel, are extracted together, leaving no target component residue in the chamber.

[0024] This invention also provides a cell processing system, including an inverted conical centrifuge chamber, a centrifuge motor driving the centrifuge chamber to rotate, an inlet pipe, an outlet pipe, and an extraction pipe respectively connected to the centrifuge chamber, an inlet pump, an outlet pump, and an extraction pump respectively installed on the three pipes, an inlet channel valve, an outlet channel valve, and an extraction channel valve respectively installed on the three pipes, and a controller respectively connected to each motor, pump, and valve. The controller is configured to execute the control method of any of the above embodiments. This system integrates the actuators and sensing interfaces required to execute the four-stage control method into one unit. The control method is deployed in the controller in a programmatic manner, and the entire cell processing process is automatically completed by the system.

[0025] This invention also provides an inverted conical centrifuge chamber assembly, comprising a chamber body that tapers from a planar end towards the bottom, a central shaft extending into the chamber body through an opening at the planar end, and a partition disposed on the inner side of the chamber body. The cross-section of the planar end is non-circular, and its periphery is provided with storage sections distributed circumferentially, which are used to contain target components when the chamber body rotates; the central shaft is provided with an inlet channel, an outlet channel, and an extraction channel; the partition is disposed near the storage sections and extends radially outward from a position near the central shaft, used to separate waste liquid from the target components when the chamber body rotates. This assembly, with the geometry of the chamber body, the cooperation between the storage sections at the periphery of the planar end and the partition, constitutes a structure for the enrichment and retention of target components, providing a structural basis for the implementation of the aforementioned control method.

[0026] Compared with the prior art, the present invention has the following technical effects: 1. By automatically connecting the four stages of liquid feeding, centrifugation, washing and extraction along the time axis, the entire process of fat matrix component processing is completed in a closed environment, replacing manual operation in multiple steps, and ensuring the consistency and sterility of the processing.

[0027] 2. By utilizing the geometric characteristics of the inverted conical chamber, which allows the fatty matrix components to accumulate on both sides of the chamber during rotation and to converge along the conical chamber wall to the bottom of the chamber by gravity after stopping, the separation and extraction processes share the same geometric characteristics of the same chamber. This eliminates the need for intermediate structures such as filters and storage units, thus avoiding filter clogging, cell wall loss, and deceleration disturbances.

[0028] 3. Through the coordination of the baffle and the drainage flow rate, the waste discharge process is carried out under the premise that the fatty matrix components are completely retained, and the target components are not lost with the waste liquid after the waste liquid is discharged.

[0029] 4. Through a resuspension process involving centrifugation-stop-centrifugation cycles, cells adhering to the wall and those settling to the bottom are resuspended and brought into full contact with the washing solution, achieving thorough washing even without a nozzle structure.

[0030] 5. By combining the extraction flow rate with the excess coefficient, the fatty matrix components that have accumulated at the bottom of the chamber, along with the pathway residues, are extracted together, leaving no target component residues in the chamber. Attached Figure Description

[0031] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein: Figure 1 This is a perspective view of the assembly structure of a cell processing system provided in an embodiment of the present invention; Figure 2 for Figure 1 The front view of the assembly structure of the cell processing system shown; Figure 3 for Figure 1 Left view of the assembly structure of the cell processing system shown. Figure 4 for Figure 2 A schematic diagram of the cross-sectional structure along the AA direction; Figure 5 for Figure 2 A schematic diagram of the cross-sectional structure along the BB direction; Figure 6 for Figure 2 A schematic diagram of the cross-sectional structure along the CC direction; Figure 7 for Figure 3 A schematic diagram of the cross-sectional structure along the DD direction; Figure 8 This is a system block diagram of a cell processing system provided in an embodiment of the present invention; Figure 9 This is a main flowchart of a control method provided in an embodiment of the present invention; Figure 10 This is a sub-flowchart of the cleaning stage provided in an embodiment of the present invention.

[0032] Explanation of reference numerals in the attached figures: 11. Inverted conical centrifuge chamber; 111. Chamber body; 112. Conical chamber wall; 113. Partition; 114. Chamber bottom; 115. Flat end; 12. Central shaft; 121. Liquid inlet channel; 122. Liquid outlet channel; 123. Extraction channel; 124. Liquid inlet connecting hole; 125. Liquid outlet connecting hole; 126. Extraction connecting hole; 127. Liquid outlet branch pipe; 128. Waste suction opening; 13. Bearing sleeve; 131. Sleeve opening; 132. Plug seal; 133. Retaining ring; 134. Gasket; 135. Bearing; 136. First annular cavity; 137. Second annular cavity; 138. Third annular cavity; 139. Annular positioning groove; 141. Liquid inlet pipe; 142. Liquid outlet pipe; 143. Extraction pipe; 151. Cryopreservation bag. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of this invention clearer, the embodiments of this invention will be further described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without inventive effort are within the scope of protection of this invention.

[0034] In the description of this invention, it should be noted that the terms "axial," "radial," and "circumferential" are defined with reference to the axis of the central axis: the direction extending along the axis of the central axis is axial, the direction perpendicular to the axis and pointing outward is radial, and the direction surrounding the axis is circumferential; "inner side" and "outer side" are defined with reference to proximity to or distance from the axis of the central axis, with the side closer to the axis of the central axis being the inner side and the side farther from the axis of the central axis being the outer side. Unless otherwise specified, the orientations and directions mentioned in the following description are based on the above reference system.

[0035] like Figure 1 As shown, this embodiment provides a cell processing system for centrifuging enzymatically hydrolyzed adipose tissue to obtain adipose matrix components (ADRCs). The cell processing system includes an inverted conical centrifuge chamber 11, a central shaft 12, a bearing sleeve 13, tubing, and a collection container. The inverted conical centrifuge chamber 11 is the controlled object for centrifugation, connected to a drive unit via the central shaft 12, which drives the inverted conical centrifuge chamber 11 to rotate around its own axis. The bearing sleeve 13 is fitted onto the outside of the central shaft 12 and is used to connect the inlet pipe 141, the outlet pipe 142, and the extraction pipe 143 to three flow channels inside the central shaft 12. The inlet pipe 141 is used to input the enzymatically hydrolyzed fat cells into the central shaft 12, the outlet pipe 142 is used to discharge waste liquid, and the extraction pipe 143 is used to extract the adipose matrix components after centrifugation. One end of the extraction pipe 143 is located outside the inverted conical centrifuge chamber 11 and connected to the extraction pump, while the other end enters the central shaft 12 and extends along the axial direction of the central shaft 12 to the tip of the inverted conical centrifuge chamber 11, and an opening communicating with the interior of the inverted conical centrifuge chamber 11 is provided on the side wall.

[0036] In this embodiment, the tip of the inverted conical centrifuge chamber 11 is located at its bottom. As the waste liquid is gradually extracted during the separation process, the fatty matrix component can enter the central shaft 12 through the opening at the bottom of the inverted conical centrifuge chamber 11, and then leave the inverted conical centrifuge chamber 11. The collection container is a cryopreservation bag 151, which is connected to the end of the extraction pipeline 143 away from the inverted conical centrifuge chamber 11, and is used to receive the extracted fatty matrix component. In actual use, the extraction pump is connected to the extraction pipeline 143, and after centrifugation, the fatty matrix component at the bottom (tip of the inverted cone) of the inverted conical centrifuge chamber 11 is aspirated and transported to the cryopreservation bag 151 for storage through the extraction pipeline 143. During storage, the pipeline can be closed first, and then the handheld tubing can be heat-sealed to keep the fatty matrix component in the cryopreservation bag 151 in a closed and sterile state.

[0037] like Figure 2 and Figure 3As shown, viewed from the outside, the inverted conical centrifuge chamber 11 is cone-shaped, wider at the top and narrower at the bottom, with its flat end 115 at the top and its bottom 114 converging at the bottom. A central shaft 12 passes axially through a bearing sleeve 13 and connects to the inverted conical centrifuge chamber 11, with both ends of the central shaft 12 extending out of the bearing sleeve 13. The sidewall of the bearing sleeve 13 has sleeve openings 131 at different heights along the axial direction, and inlet pipes 141 and outlet pipes 142 are connected to the corresponding sleeve openings 131. The inlet pipe 141 inputs enzymatically hydrolyzed fat cells into the central shaft 12 through its connected sleeve opening 131, and the outlet pipe 142 discharges waste liquid through its connected sleeve opening 131. Figure 2 From the frontal view shown, the connection port on one side of the drain pipe 142 and the portion of the central shaft 12 extending out of the bearing sleeve 13 are visible; Figure 3 As shown in the left-hand view, the arrangement of the drain pipe 142 on the other side of the central axis 12 can be seen.

[0038] In actual use, the bearing sleeve 13 is fixed to the frame of the cell processing system (not shown in the figure) and remains stationary, while the central shaft 12 rotates relative to the bearing sleeve 13. Thus, the externally stationary inlet pipe 141, outlet pipe 142 and extraction pipe 143 are connected to the inverted conical centrifuge chamber 11 that rotates with the central shaft 12, and fluid communication is achieved in the rotating state through the annular cavity inside the bearing sleeve 13, so that the pipes do not become entangled.

[0039] like Figure 4 As shown, along Figure 2 A cross-section along the AA direction reveals the internal structure of the inverted conical centrifuge chamber 11. The chamber body 111 of the inverted conical centrifuge chamber 11 tapers from the planar end 115 towards the bottom 114, with conical walls 112 forming the side walls of the chamber body 111. The cross-section of the planar end 115 is non-circular; in this embodiment, the cross-section of the planar end 115 is elliptical, with two circumferentially distributed storage sections formed at both ends of the major axis of the ellipse. These storage sections contain the densest adipose matrix component when the chamber body 111 rotates.

[0040] A partition 113 is disposed on the inner side of the chamber 111 and near the storage section. The partition 113 is symmetrically arranged on both sides of the central axis 12 near the tip of the ellipse, and slightly protrudes radially outward along the central axis 12, forming an arc approximately circular (centered on the central axis 12). The partition 113 is used to separate the waste liquid from the fatty matrix components during centrifugation. As the centrifuge chamber 11 rotates, the waste liquid rises along the conical chamber wall 112 under centrifugal force. After stratification, the waste liquid is located on the side of the partition 113 facing the central axis 12, just touching the top plane of the inner wall of the chamber 111, and is sucked out through the opening there.

[0041] In this embodiment, a drain branch pipe 127 is also provided inside the chamber 111. Multiple drain branch pipes 127 are provided and extend from the central axis 12, connecting the interior of the chamber 111 via a flat end 115. The drain branch pipe 127 enters the interior of the chamber 111 adjacent to the partition 113. The end of the drain branch pipe 127 is provided with a waste suction opening 128, located on the side of the partition 113 facing the central axis 12, i.e., at the top plane of the inner wall that comes into contact with the stratified waste liquid. When the waste liquid is extracted, it rises along the conical chamber wall 112 under centrifugal force, crosses the boundary of the area defined by the partition 113, enters the location of the waste suction opening 128, and is extracted.

[0042] In actual use, when the inverted conical centrifuge chamber 11 rotates, the densest fatty matrix component is thrown to both sides of the chamber 111, i.e., where the two storage sections are located, and is retained on one side of the storage section by the partition 113; the less dense waste liquid (free lipid droplets, water, enzymes, etc.) is located on the inner side, i.e., the side of the partition 113 facing the central axis 12. The partition 113 thus radially separates the fatty matrix component from the waste liquid, limiting the volume of the fatty matrix component to the set range corresponding to the storage section. The area used to retain the fatty matrix component during centrifugation is located between the partition 113 and the storage section.

[0043] The side wall of the central shaft 12 has an opening at the position corresponding to the bottom of the chamber 114. The opening connects the extraction channel 123 with the interior of the chamber 111, so that the fatty matrix components gathered at the bottom of the chamber 114 can enter the extraction channel 123 through the opening and be extracted.

[0044] like Figure 5 As shown, along Figure 2 A cross-section along the BB direction reveals the connection between the annular cavities inside the bearing sleeve 13 and the flow channels inside the central shaft 12. The bearing sleeve 13 is fitted onto the outside of the central shaft 12 and rotates with it. Multiple annular cavities are formed between the bearing sleeve 13 and the central shaft 12, distributed axially along the central shaft 12, including a first layer of annular cavities 136, a second layer of annular cavities 137, and a third layer of annular cavities 138. The central shaft 12 contains, along its axial direction, independent inlet channels 121, outlet channels 122, and extraction channels 123.

[0045] The second annular cavity 137 is connected to the liquid inlet channel 121 and is connected to the interior of the central shaft 12 through the liquid inlet connecting hole 124. The second annular cavity 137 is also connected to the liquid inlet pipe 141 through the sleeve opening 131 at the corresponding height on the side wall of the bearing sleeve 13, thus forming the liquid inlet flow path: liquid inlet pipe 141, sleeve opening 131, second annular cavity 137, liquid inlet connecting hole 124, liquid inlet channel 121, which finally enters the inverted conical centrifuge chamber 11.

[0046] The third annular cavity 138 is connected to the extraction channel 123 and is connected to the interior of the central shaft 12 through the extraction connecting hole 126. The third annular cavity 138 is also connected to the extraction pipeline 143 through the sleeve opening 131 at the corresponding height on the side wall of the bearing sleeve 13, thus forming the liquid extraction path: extraction pipeline 143, sleeve opening 131, third annular cavity 138, extraction connecting hole 126, extraction channel 123, and finally leads to the opening at the bottom (tip) of the inverted conical centrifuge chamber 11.

[0047] like Figure 6 As shown, along Figure 2 A cross-section along the CC direction reveals the formation of each annular cavity and the waste liquid discharge path. In this embodiment, multiple seals are provided between the bearing sleeve 13 and the central shaft 12, and these seals are stacked along the axial direction of the central shaft 12. The seals are plug seals 132, of which four are provided; and two retaining rings 133 are provided. Each plug seal 132 is seated within an annular positioning groove 139 provided on the inner wall of the bearing sleeve 13. Several annular positioning grooves 139 are distributed along the axial direction of the inner wall of the bearing sleeve 13 to limit the axial position of the plug seal 132. A retaining ring 133 is sandwiched between two adjacent plug seals 132, and at least one annular cavity is formed between the retaining ring 133 and the central shaft 12.

[0048] Specifically, along the axial direction of the bearing sleeve 13 from one end to the other, the stacking order of the components is as follows: bearing 135, gasket 134, plug seal 132, retaining ring 133, plug seal 132, retaining ring 133, plug seal 132, plug seal 132. Notably, no retaining ring 133 is placed between the last two adjacent plug seals 132; instead, a third annular cavity 138 is directly formed. The remaining two annular cavities (first annular cavity 136 and second annular cavity 137) are formed between the retaining ring 133 and the central shaft 12. Bearings 135 are respectively provided at both ends of the bearing sleeve 13. The bearings 135 support the rotation of the central shaft 12 relative to the bearing sleeve 13. A gasket 134 is provided between the bearing 135 and the plug seal 132 located at the far end. Thus, the bearing sleeve 13 remains stationary, while the central shaft 12, along with the inverted conical centrifugal chamber 11, rotates. During rotation, each annular cavity maintains fluid communication in its corresponding flow path.

[0049] The first annular cavity 136 is connected to the drain channel 122 and to the interior of the central shaft 12 through the drain connection hole 125. Simultaneously, the first annular cavity 136 is connected to the drain pipe 142 through a sleeve opening 131 at a corresponding height on the side wall of the bearing sleeve 13. The other end of the drain channel 122 is connected to a drain branch pipe 127 extending from the central shaft 12, which enters the interior of the inverted conical centrifuge chamber 11 via a flat end 115. This constitutes the drain flow path: waste liquid enters the drain branch pipe 127 through the waste suction opening 128, passes through the drain channel 122, the drain connection hole 125, the first annular cavity 136, and the sleeve opening 131, and is finally discharged through the drain pipe 142.

[0050] The three flow paths are connected to each other through three independent annular cavities. The liquid inlet, liquid outlet, and extraction do not cross-flow during rotation, thus maintaining the integrity of the closed environment.

[0051] like Figure 7 As shown, along Figure 3 A cross-section along the DD direction reveals the orientation of the extraction conduit 143 and the extraction path of the adipose matrix components. One end of the extraction conduit 143 is located outside the inverted conical centrifuge chamber 11 and connected to the extraction pump. The other end enters the central shaft 12 and extends axially along the central shaft 12 to the tip of the inverted conical centrifuge chamber 11, with an opening on the side wall connecting to the interior of the inverted conical centrifuge chamber 11. This opening is located at the bottom 114 of the chamber and communicates with the extraction channel 123.

[0052] In actual use, when the extraction stage begins and the centrifuge motor stops rotating, the fatty matrix components slide down the conical chamber wall 112 under the action of gravity and collect at the bottom (i.e., the tip) of the inverted conical centrifuge chamber 11. At this time, the extraction pump is started and the extraction channel valve is opened. The extraction pump extracts the fatty matrix components collected at the bottom 114 of the chamber at the set extraction flow rate. The fatty matrix components enter the extraction channel 123 through the opening at the bottom 114 of the chamber, and then enter the extraction pipeline 143 through the extraction connecting hole 126, the third annular cavity 138, and the sleeve opening 131, and are finally transported to the cryopreservation bag 151. Since the central shaft 12 rotates synchronously with the inverted conical centrifuge chamber 11 before the extraction pump is started, the opening at the bottom 114 of the chamber sweeps across the bottom area of ​​the chamber with the rotation, so that the fatty matrix components collected at various points on the bottom of the chamber can be attracted and collected by the opening in a circular motion, reducing the residue at the bottom of the chamber.

[0053] The inner diameter of the extraction tube 143 is smaller than the inner diameters of the inlet tube 141 and the outlet tube 142. The volume of the extracted fat matrix component is limited (e.g., about 10 ml). The smaller inner diameter of the tube reduces the volume of liquid retained in the tube, thereby reducing the residual loss of the extracted fat matrix component in the tube and avoiding waste of the target component.

[0054] like Figure 8As shown, the cell processing system also includes a centrifugal motor, an inlet pump, a outlet pump, an extraction pump, inlet channel valves, outlet channel valves, extraction channel valves, an optical sensor, a pressure sensor, a speed and vibration sensor, and a controller. The centrifugal motor drives the central shaft 12 and the inverted conical centrifuge chamber 11 to rotate. The inlet pump, outlet pump, and extraction pump are respectively installed on the inlet pipe 141, outlet pipe 142, and extraction pipe 143, providing power for the three flow paths. The inlet channel valve, outlet channel valve, and extraction channel valve are respectively installed on the three pipes to control the opening and closing of the corresponding flow paths. The optical sensor is installed on the inlet pipe 141 to detect the state of the liquid in the pipe. The pressure sensor is installed on the three pipes to monitor for blockages or ruptures in the pipes. The speed and vibration sensor is installed at the centrifugal motor to monitor the speed and vibration. The controller is connected to the centrifugal motor, each pump, each valve, and each sensor, receiving detection signals from each sensor and outputting control commands to the centrifugal motor, each pump, and each valve. Figure 8 Each function box is directly represented by the text within the box, without any additional annotations in the attached diagram.

[0055] like Figure 9 As shown, the controller executes the liquid feeding stage S1, centrifugation stage S2, washing stage S3, and extraction stage S4 in chronological order. The following is in conjunction with... Figure 9 The control process at each stage is explained.

[0056] In the liquid inlet stage S1, the controller opens the liquid inlet channel valve and starts the liquid inlet pump, delivering the mixture into the inverted conical centrifuge chamber 11 at the set liquid inlet flow rate. The mixture is a mixture of the enzymatically hydrolyzed fat matrix components and LR solution. The liquid inlet flow rate is medium, for example, 200 ml / min. The controller records the running time Tcellin of the liquid inlet pump, and the liquid inlet volume Vcellin is the product of the liquid inlet flow rate and the running time Tcellin.

[0057] The reason for using a medium inlet flow rate is that the inlet endpoint needs to be detected based on the liquid state during the inlet process. If the pump speed is too high, the instantaneous speed of the fluid flowing past the optical sensor will exceed the response time of the system signal processing and valve mechanical action. Once the optical sensor detects the grease state, before the system completely closes the inlet channel valve, an uncontrollable volume of excess grease will actually be pushed into the inverted conical centrifuge chamber 11 by inertia, thus compromising the purity of the extraction. Conversely, if the pump speed is too slow, it will increase the processing time. Therefore, the inlet flow rate is set to a medium speed that matches the signal processing time of the optical sensor and the action time of the inlet channel valve, so that the pumping volume between the moment the optical sensor detects the state change and the moment the valve actually closes is controlled within an acceptable range.

[0058] The liquid inlet endpoint employs dual determination conditions. First, an optical sensor detects the state of the liquid in the inlet pipe 141. The mixture contains fatty matrix components and solution, and its optical state differs from that of grease. When the mixture is completely delivered and the liquid state in the pipe changes from a mixed state to a grease state, it indicates that the fatty matrix components have entered the inverted conical centrifuge chamber 11. The controller then stops the inlet pump and closes the inlet channel valve. Second, when the inlet volume Vcellin reaches the set capacity threshold Vmax, the controller also stops the inlet pump and closes the inlet channel valve. The capacity threshold Vmax corresponds to the capacity of the inverted conical centrifuge chamber 11. For example, if the volume of the inverted conical centrifuge chamber 11 is 300ml, then the capacity threshold Vmax is 300ml. The inlet process terminates when either determination condition is met, providing redundancy and ensuring that the inlet process neither over-increases nor relies on a single detection method.

[0059] During the liquid inlet process, a pressure sensor monitors the inlet pressure in real time. When the inlet pressure exceeds the set pressure threshold Pmax and remains at that level for a set duration, a blockage in the rotary seal structure or pipeline is detected, triggering an alarm and shutting down the machine. The pressure threshold Pmax is, for example, 110% of the reference value, and the holding time is, for example, 1 second. The continuous increase in inlet pressure is a direct reflection of blockage. This protection ensures that the blockage is addressed before it causes pump overload or pipeline rupture, preventing disruption of the closed environment.

[0060] After the liquid inlet stage S1, the centrifugation separation stage S2 begins. The controller controls the centrifuge motor to accelerate to the set acceleration to reach the separation speed and maintain it for the set separation time. The acceleration is set, for example, to uniformly increase from 0 to 3000 rpm within 30 seconds; the separation speed is set, for example, to 3000 rpm; and the separation time is set, for example, to 10 minutes. Under continuous centrifugal force, the mixture separates into layers based on density differences: the densest fatty matrix component is thrown towards the two storage sections at the largest diameter points on both sides of the chamber 111, i.e., the periphery of the planar ends 115; the waste liquid (free lipid droplets, water, enzymes, and other less dense substances) is suspended in the inner layer near the central axis 12. The volume of the retained fatty matrix component is denoted as Vcell, for example, 5 ml on one side, totaling 10 ml on both sides.

[0061] During centrifugal motor operation, a speed sensor performs closed-loop control of the speed, ensuring that speed fluctuations are within a set range, such as ±1%. The stability of the speed determines the stability of the centrifugal force field, which in turn determines the repeatability of the stratification position. A vibration sensor simultaneously monitors dynamic balance; when the amplitude exceeds the limit Amax, the controller automatically stops the machine. The amplitude threshold Amax is, for example, 110% of the reference value.

[0062] At a set waste discharge point during the separation speed maintenance process, the controller opens the drain channel valve and starts the drain pump to discharge the waste liquid from the inverted conical centrifuge chamber 11 at a set discharge flow rate. The waste discharge point is, for example, the 5th minute of maintaining the separation speed, at which point the waste liquid has completed stratification and risen to the height region of the waste suction opening 128. The discharge flow rate is low, for example, 100 ml / min. The running time of the drain pump is set based on the volume of the waste liquid to be discharged and the discharge flow rate. The volume of the waste liquid to be discharged is the difference between the centrifuge chamber's upper capacity limit Vmax or the actual influent volume Vin minus the volume of the retained fat matrix component Vcell. The discharge time = this difference / discharge flow rate.

[0063] The low discharge flow rate is adopted because the baffle 113 inside the inverted conical centrifuge chamber 11 acts as a barrier to the fatty matrix components: during normal waste discharge, the baffle 113 prevents the fatty matrix components from being discharged; however, if the suction force of the discharge pump is too strong, the fatty matrix components will be attracted and climb over the baffle 113, and be lost with the waste liquid. Therefore, the discharge flow rate is set below the critical value that would attract the fatty matrix components to climb over the baffle 113, so that the fatty matrix components are completely retained while the waste liquid is discharged. Under the action of centrifugal force, the waste liquid rises upward along the conical chamber wall 112, and after stratification, it is located on the side of the baffle 113 facing the central axis 12, just touching the top plane of the inner wall of the chamber 111, and is sucked out by the waste suction opening 128, and discharged outside the chamber through the discharge branch pipe 127, the discharge channel 122, the first annular cavity 136, and the discharge pipe 142.

[0064] After the separation period ends, the controller decelerates the centrifuge motor to zero speed with a set acceleration, for example, from 3000 rpm to 0 rpm within 30 seconds. During deceleration and stopping, the fatty matrix components lose the constraint of centrifugal force and fall along the conical wall 112 under the action of gravity, accumulating at the bottom of the inverted conical centrifuge chamber 11. The tapering geometry of the inverted conical wall 112 ensures that regardless of the height at which the fatty matrix components accumulate on the wall, their final destination due to gravity is the bottom 114. This geometric characteristic provides the structural basis for the aggregation extraction in the extraction stage.

[0065] like Figure 10 As shown, the cleaning stage S3 includes conveying the cleaning solution, resuspension, recentrifugation, and waste discharge, and can be repeated a set number of times. The following is combined with... Figure 10 The cleaning stage S3 is explained.

[0066] In the cleaning phase S3, the controller opens the inlet valve and starts the inlet pump to deliver the cleaning solution into the inverted conical centrifuge chamber 11 at a set cleaning flow rate. The cleaning flow rate is high, for example, 300 ml / min. The cleaning solution is an LR solution. The controller sets the volume of the cleaning solution based on the capacity of the inverted conical centrifuge chamber 11 and the volume of the fatty matrix components: the running time Tlrin of the inlet pump is calculated based on the maximum capacity, for example, Tlrin = (Vmax - Vcell) / cleaning flow rate, ensuring that the cleaning solution fills the space within the chamber not occupied by the fatty matrix components, achieving full contact with them.

[0067] After the cleaning solution is injected, the controller performs a resuspension operation. The resuspension operation is a short-term centrifugation-stop-centrifugation cycle: the controller increases the centrifuge motor speed from zero to the set resuspension speed within a set time period, then immediately reduces the speed to zero within the set time period, repeating this cycle a set number of times. For example, the speed is increased from 0 to 600 rpm within 1 second, then immediately reduced from 600 rpm to 0 rpm within 1 second, repeated 15 times. The purpose of the resuspension operation is to resuspend cells that have adhered to the walls and settled to the bottom: after the previous centrifugation, some lipid matrix components adhere to the chamber walls, and some settle to the bottom. If the cleaning solution is injected statically, these cells are unlikely to come into contact with the cleaning solution. Through the centrifugation-stop-centrifugation cycle, the liquid in the chamber repeatedly experiences acceleration and stillness. Under the action of acceleration changes, the cells that have adhered to the walls and settled to the bottom detach from their attachment positions and re-enter a suspended state, thus making full contact with the cleaning solution. Since the cleaning solution is a newly added liquid that has not yet been mixed with the fatty matrix components, the resuspension action allows it to be fully thrown into the area where the fatty matrix components are located and mixed with them, ensuring thorough cleaning.

[0068] After the resuspension action is completed, the controller controls the centrifuge motor to accelerate again, reaching the separation speed at a set acceleration and maintaining it for a set separation time, for example, increasing from 0 to 3000 rpm within 30 seconds and maintaining it for 10 minutes, allowing the washing liquid and the fat matrix components to separate again by centrifugation. Subsequently, while maintaining the separation speed, at a set waste discharge point (for example, at the 5th minute), the controller opens the drain channel valve and starts the drain pump to discharge the waste liquid at a set discharge flow rate (for example, 100 ml / min). The discharge time is set, for example, by dividing the difference between the centrifuge chamber's maximum capacity Vmax or the actual inflow volume Vin and the volume of the retained fat matrix component Vcell by the discharge flow rate. During this waste discharge process, the baffle 113 blocks the fat matrix components, and the low-speed discharge prevents the fat matrix components from being attracted by suction and climbing over the baffle 113. Its mechanism is consistent with the online waste discharge in the centrifugation separation stage S2.

[0069] After the separation period ends, the controller controls the centrifuge motor to reduce its speed to zero at a set acceleration. The controller determines whether the number of repetitions in the washing stage S3 has reached the set number of times (e.g., 3 times): if not, it returns to the next washing cycle, i.e., re-delivering the washing solution, resuspending, centrifuging, and discharging waste; if the number of times has been reached, it proceeds to the extraction stage S4. Through multiple washing cycles, residual enzymes, free lipid droplets, and other impurities are gradually diluted and discharged with each wash, and the purity of the lipid matrix components gradually increases.

[0070] In extraction stage S4, after the final deceleration stop during the final rinse, the fatty matrix components have accumulated at the bottom of the inverted conical centrifuge chamber 111 under gravity along the conical chamber wall 112. The controller opens the extraction channel valve and starts the extraction pump, discharging the accumulated fatty matrix components at the set extraction flow rate. The components are then transported to the cryopreservation bag 151 via the extraction channel 123, the third annular cavity 138, and the extraction tubing 143. The extraction flow rate is ultra-low, for example, 30 ml / min. The extraction pump's runtime is set to excess discharge to prevent fatty matrix components from remaining in the inverted conical centrifuge chamber 11 and the extraction flow path: the runtime is set based on the volume of the fatty matrix components and the set excess coefficient, for example, runtime = 150% multiplied by (Vcell / extraction flow rate). The ultra-low speed setting minimizes disturbance to the fatty matrix components gathered at the bottom of the chamber during the extraction process, preventing the aspiration of residual waste liquid. The excess coefficient ensures that the extraction volume is slightly larger than the actual volume of the fatty matrix components, extracting any residual parts in the extraction path and guaranteeing that no target components remain in the chamber. After extraction, the pipeline is closed and the cryopreservation bag 151 is heat-sealed, preserving the fatty matrix components in a closed and sterile state.

[0071] Thus, the liquid feeding stage S1, centrifugation stage S2, washing stage S3 and extraction stage S4 are completed automatically in sequence along the time axis. The enrichment, washing and extraction of fatty matrix components are achieved in a closed environment within the same inverted conical centrifuge chamber 11, without the need for manual operation in multiple steps.

[0072] It should be noted that the values ​​of rotation speed, flow rate, duration, volume, pressure threshold, amplitude threshold, and number of repetitions given in the above stages are all illustrative examples. Those skilled in the art can adjust the above values ​​within a reasonable range according to the volume of the inverted conical centrifuge chamber, the amount of fat matrix components to be processed, and the target purity requirements. Such adjustments do not depart from the spirit and scope of the present invention.

[0073] It should be noted that the above-mentioned multiple stages and implementation methods are merely examples, and the technical solutions in each implementation method can be combined, all of which are within the protection scope of this invention.

[0074] In the description of this invention, the terms "upper," "lower," "horizontal," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0075] It should be noted that in the description of this invention, the terms "first," "second," etc., are used only to distinguish the objects of description and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated.

[0076] The control method for the cell processing system based on an inverted conical centrifuge chamber provided by this invention has been described in detail above. Any obvious modifications made by those skilled in the art without departing from the essence of this invention will constitute an infringement of the patent rights of this invention and will incur corresponding legal liability.

Claims

1. A control method for a cell processing system, applied to a cell processing system comprising an inverted conical centrifuge chamber and a centrifuge motor for driving the centrifuge chamber to rotate, characterized in that, The control method includes the following stages executed sequentially over time: S1, Liquid Inlet Stage: The mixture of the fat matrix components and the solution is delivered into the centrifuge chamber; S2, Centrifugal Separation Stage: Control the centrifugal motor to rotate at a set separation speed and maintain the set separation time, so that the fatty matrix component in the mixture and the waste liquid are centrifuged and separated, and the waste liquid is discharged from the centrifugal chamber; S3, Cleaning stage: The cleaning solution is delivered into the centrifuge chamber, the centrifuge motor is controlled to rotate, the cleaning solution and the fat matrix components are centrifuged and separated, and the waste liquid is discharged from the centrifuge chamber; The cleaning phase is performed at least once; S4. Extraction stage: Control the centrifuge motor to stop rotating, and the fatty matrix components gather at the bottom of the centrifuge chamber along the conical wall of the centrifuge chamber under the action of gravity. The gathered fatty matrix components are discharged into the collection container.

2. The control method according to claim 1, characterized in that, The liquid inlet stage includes: opening the liquid inlet channel valve and starting the liquid inlet pump to deliver the mixture into the centrifuge chamber at a set liquid inlet flow rate; and recording the running time of the liquid inlet pump to obtain the liquid inlet volume of the mixture.

3. The control method according to claim 2, characterized in that, The control method further includes: detecting the state of the liquid in the inlet pipeline using an optical sensor; when the optical sensor detects that the state of the liquid changes from the state of the mixture to the state of grease, controlling the inlet pump to stop and closing the inlet channel valve.

4. The control method according to claim 2, characterized in that, The control method further includes: when the liquid inlet volume reaches a set capacity threshold, controlling the liquid inlet pump to stop and closing the liquid inlet channel valve.

5. The control method according to claim 2, characterized in that, The control method further includes: monitoring the inlet pressure; when the inlet pressure exceeds the set pressure threshold and continues for a set time, determining that the rotary seal structure or pipeline is blocked, triggering an alarm and stopping the machine.

6. The control method according to claim 1, characterized in that, During the centrifugal separation stage, discharging waste liquid from the centrifuge chamber includes: while maintaining the separation speed, opening the drain channel valve and starting the drain pump at a set waste discharge time point to discharge the waste liquid from the centrifuge chamber.

7. The control method according to claim 6, characterized in that, The centrifuge chamber is equipped with a partition for separating the waste liquid from the fatty matrix component during centrifugation; the drain pump discharges the waste liquid at a set drain flow rate, which is set so that the fatty matrix component is not attracted by the suction of the drain pump and climbs over the partition.

8. The control method according to claim 6, characterized in that, The operating time of the drainage pump is set according to the volume of waste liquid to be discharged and the drainage flow rate.

9. The control method according to claim 1, characterized in that, The control method further includes: performing closed-loop control on the speed of the centrifugal motor to keep the speed fluctuation within a set fluctuation range.

10. The control method according to claim 1, characterized in that, The control method further includes: monitoring the vibration amplitude of the centrifugal motor; and controlling the centrifugal motor to stop when the vibration amplitude exceeds a set amplitude threshold.

11. The control method according to claim 1, characterized in that, During the cleaning stage, delivering the cleaning fluid into the centrifuge chamber includes: starting the inlet pump to deliver the cleaning fluid into the centrifuge chamber at a set cleaning flow rate; the volume of the cleaning fluid is set according to the capacity of the centrifuge chamber and the volume of the fatty matrix component.

12. The control method according to claim 1, characterized in that, The cleaning stage also includes a resuspension action: controlling the centrifuge motor to perform a centrifugation-stop-centrifugation cycle to resuspend the cells that are attached to the wall and settled to the bottom.

13. The control method according to claim 12, characterized in that, The resuspension action includes: increasing the speed of the centrifugal motor from zero to a set resuspension speed within a set time period, and then reducing the speed from the resuspension speed to zero within a set time period; the resuspension action is repeated a set number of cycles.

14. The control method according to claim 1, characterized in that, The cleaning stage is repeated a set number of times, and each execution includes delivering the cleaning fluid, centrifugation and stratification, and discharging the waste liquid from the centrifuge chamber.

15. The control method according to claim 1, characterized in that, In the extraction stage, discharging the collected adipose matrix components into the collection container includes: opening the extraction channel valve and starting the extraction pump to discharge the adipose matrix components at a set extraction flow rate; the running time of the extraction pump is set according to the volume of the adipose matrix components and a set excess coefficient.

16. A cell processing system, characterized in that, include: Inverted cone-shaped centrifuge chamber; A centrifugal motor drives the centrifuge chamber to rotate; The inlet pipe, outlet pipe, and extraction pipe are respectively connected to the centrifuge chamber; An inlet pump, a drain pump, and an extraction pump are respectively installed in the inlet pipeline, the drain pipeline, and the extraction pipeline; The inlet valve, the outlet valve, and the extraction valve are respectively installed in the inlet pipeline, the outlet pipeline, and the extraction pipeline; as well as The controller is connected to the centrifugal motor, the inlet pump, the outlet pump, the extraction pump, the inlet channel valve, the outlet channel valve, and the extraction channel valve, respectively. The controller is configured to perform the control method of the cell processing system as described in any one of claims 1 to 15.

17. The cell processing system according to claim 16, characterized in that, It also includes an optical sensor, which is disposed in the liquid inlet pipe. The optical sensor detects the state of the liquid in the liquid inlet pipe and outputs a detection signal to the controller.

18. The cell processing system according to claim 16, characterized in that, It also includes a pressure sensor, which is disposed in at least one of the inlet pipe, the outlet pipe and the extraction pipe. The pressure sensor monitors the blockage or rupture of the pipe in which it is located and outputs a detection signal to the controller.

19. The cell processing system according to claim 16, characterized in that, It also includes a speed and vibration sensor, which monitors the speed and vibration of the centrifugal motor and outputs a detection signal to the controller.

20. The cell processing system according to claim 16, characterized in that, It also includes a central shaft and a bearing sleeve; the central shaft is connected to the centrifuge chamber and driven to rotate by the centrifuge motor, and the central shaft is provided with an inlet channel, an outlet channel and an extraction channel; the bearing sleeve is sleeved on the outside of the central shaft and rotates with the central shaft, and the bearing sleeve and the central shaft form a plurality of annular cavities distributed along the axial direction of the central shaft; each annular cavity is respectively connected to one of the inlet channel, the outlet channel and the extraction channel, and is respectively connected to one of the inlet pipe, the outlet pipe and the extraction pipe.

21. The cell processing system according to claim 20, characterized in that, Multiple seals are provided between the bearing sleeve and the central shaft. The multiple seals are stacked along the axial direction, and each seal is seated in an annular positioning groove provided on the inner wall of the bearing sleeve. A retaining ring is sandwiched between two adjacent seals, and at least one annular cavity is formed between the retaining ring and the central shaft.

22. The cell processing system according to claim 21, characterized in that, Bearings are provided at both ends of the bearing sleeve, and the bearings support the rotation of the central shaft relative to the bearing sleeve; a gasket is provided between the bearing and the seal located at the far end.

23. The cell processing system according to claim 16, characterized in that, The centrifuge chamber is equipped with a partition that divides the internal space of the centrifuge chamber to limit the volume of the fat matrix components within a set range.

24. The cell processing system according to claim 16, characterized in that, The inner diameter of the extraction pipeline is smaller than the inner diameter of the inlet pipeline and the outlet pipeline; the cell processing system also includes a cryopreservation bag, which is connected to the end of the extraction pipeline away from the centrifuge chamber.

25. An inverted conical centrifuge chamber assembly, characterized in that, include: The cabin body tapers from its planar end toward its bottom, the planar end has an opening, the cross-section of the planar end is non-circular, and the periphery of the planar end has storage sections distributed circumferentially, the storage sections being used to contain the target component when the cabin body rotates; A central shaft extends into the chamber through the opening, and the central shaft is provided with an inlet channel, an outlet channel, and an extraction channel; and A partition is disposed on the inner side of the chamber and near the storage section. The partition extends radially outward from a position near the central axis. The partition is used to separate the waste liquid from the target component when the chamber rotates.

26. The inverted conical centrifuge chamber assembly according to claim 25, characterized in that, It also includes multiple drainage branches extending from the central axis, which connect to the interior of the cabin via the planar end; the end of the drainage branch is provided with a waste suction opening, which is located on the side of the partition facing the central axis.

27. The inverted conical centrifuge chamber assembly according to claim 25, characterized in that, The side wall of the central shaft has an opening at a position corresponding to the bottom of the chamber, and the opening connects the extraction channel to the interior of the chamber.

28. The inverted conical centrifuge chamber assembly according to claim 25, characterized in that, It also includes a bearing sleeve, which is sleeved on the outside of the central shaft and rotates with the central shaft; the bearing sleeve and the central shaft form a plurality of annular cavities distributed along the axial direction of the central shaft, and each annular cavity is respectively connected to one of the liquid inlet channel, the liquid outlet channel, and the extraction channel.

29. The inverted conical centrifuge chamber assembly according to claim 28, characterized in that, Multiple seals are provided between the bearing sleeve and the central shaft. The multiple seals are stacked along the axial direction, and each seal is seated in an annular positioning groove provided on the inner wall of the bearing sleeve. A retaining ring is sandwiched between two adjacent seals, and at least one annular cavity is formed between the retaining ring and the central shaft. Bearings are provided at both ends of the bearing sleeve. The bearings support the rotation of the central shaft relative to the bearing sleeve, and a gasket is provided between the bearing and the seal located at the far end.

30. The inverted conical centrifuge chamber assembly according to claim 25, characterized in that, The storage unit has two units, which are symmetrically arranged on both sides of the central axis.

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