An extraction device for detecting an antitumor pharmaceutical ingredient

Abstract

This invention relates to the field of physical analysis and detection technology, specifically to an extraction device for detecting antitumor drug components. The device includes a fixed tube body with a partition dividing the interior into an injection chamber and an adjustment chamber. A sleeve is located at the bottom of the fixed tube body, containing a solid extraction column. The sleeve is equipped with a waste collection component and a target component collection component. Several outlets are located at the bottom of the injection chamber corresponding to the position of the solid extraction column. The waste collection component is also connected to a negative pressure drive. When activated, the negative pressure drive creates a negative pressure gradient along the adjustment chamber to the waste collection component, driving the solvent to be extracted radially from the injection chamber outwards through the solid extraction column and into the waste collection component. A viscosity acquisition component is connected to the injection chamber. This invention utilizes a method where the solvent to be extracted passes through the solid extraction column along the radial thickness of the packing layer. Compared to the traditional axial flow method, this reduces the flow resistance of solid-phase extraction and solves the problem of column bed clogging caused by abnormal viscosity in biological samples.

Description

Technical Field

[0001] This invention relates to the field of physical analysis and detection technology, specifically to an extraction device for detecting antitumor drug components. Background Technology

[0002] In extraction techniques for detecting antitumor drug components, solid-phase extraction (SPE) is a commonly used sample pretreatment technique in the detection of herbal drugs. It utilizes a solid-phase extraction column filled with a specific adsorbent to selectively retain, wash, and elute target antitumor components in complex plant extracts, thereby achieving separation and enrichment. Currently, conventional SPE methods mostly rely on manual operation, and the standard operating procedure includes sample preparation (diluting the original extract to a suitable solvent system), solid-phase extraction column selection and pretreatment (activation, equilibration), sample loading, washing, and elution.

[0003] In the aforementioned manual process, the most commonly used consumable is a disposable column-type solid-phase extraction vessel. These vessels typically consist of a polypropylene column, two layers of polyethylene sieves, and adsorbent packing sandwiched between the sieves. Their purpose is to allow the sample or solvent to smoothly pass through the packing bed under gravity, negative pressure, or positive pressure, enabling the target analyte (antitumor drug component) in the sample to be adsorbed, while interfering substances are discarded with the effluent, thus rapidly achieving the extraction and concentration of the antitumor drug component.

[0004] When this technology is applied to the extraction of antitumor drug components from clinical biological samples (such as blood and plasma from different patients), the viscosity of biological samples varies among individuals. For example, patients with advanced cancer often have hyperlipidemia or hyperproteinemia, and their plasma viscosity can increase to 2-3 times the normal level. When such high-viscosity samples are manually injected or driven by negative pressure through a solid-phase extraction column, slow flow rates or even column clogging inevitably occur, affecting extraction efficiency. Therefore, it is necessary to propose an extraction device for detecting antitumor drug components to solve the above-mentioned technical problems. Summary of the Invention

[0005] To address the aforementioned issues, this invention provides an extraction device for detecting antitumor drug components. The device utilizes a method where the solvent of the raw material to be extracted passes through the solid-phase extraction column along the radial thickness of the packing layer. Compared to the traditional axial flow method, this reduces the flow resistance of solid-phase extraction and solves the problem of biological samples easily clogging the column bed due to abnormal viscosity.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: an extraction device for detecting antitumor drug components, comprising a fixed tube body, wherein a coaxially arranged partition cylinder is fixedly connected inside the fixed tube body, and the partition cylinder divides the interior of the fixed tube body into an inner injection chamber and an outer ring-shaped adjustment chamber.

[0007] The bottom of the fixed tube is provided with a sleeve body, and a frustum-shaped solid extraction column is placed inside the sleeve body. The solid extraction column is filled with adsorbent packing material. The sleeve body is provided with a waste collection component and a target component collection component. The waste collection component is used to collect the liquid phase components that are not adsorbed after passing through the solid extraction column, and the target component collection component is used to collect the liquid phase components containing the target anti-tumor drug components.

[0008] The bottom of the injection chamber extends to the inner wall of the solid extraction column. Several liquid outlets are opened at the bottom of the injection chamber corresponding to the position of the solid extraction column. The waste collection component is also connected to a negative pressure drive. The negative pressure drive is connected to a control system. When the negative pressure drive is activated, a negative pressure gradient is formed along the adjustment chamber to the waste collection component, driving the solvent of the raw material to be extracted to pass through the solid extraction column radially from the injection chamber to the waste collection component.

[0009] The top of the injection chamber is connected to a viscosity acquisition component for collecting viscosity data of the solvent of the raw material to be extracted. The fixed tube is equipped with a liquid filling component for adjusting the viscosity of the solvent of the raw material to be extracted. Both the injection chamber and the adjustment chamber are connected to the liquid filling component. Both the viscosity acquisition component and the liquid filling component are connected to the control system signal.

[0010] The technical principle of the above scheme is as follows: This scheme divides the internal space into an inner injection chamber and an outer annular regulating chamber by coaxially setting a partition cylinder inside the fixed tube. A sleeve body is detachably connected to the bottom of the fixed tube body, and the sleeve body contains a solid extraction column with a frustum-shaped annular structure. The bottom end of the injection chamber extends to the inner wall of the solid extraction column and has a liquid outlet. The waste collection component is connected to a negative pressure drive. After the control system starts the negative pressure drive, a radial negative pressure gradient is formed between the injection chamber and the waste collection chamber, driving the solvent of the raw material to be extracted to enter from the liquid outlet at the bottom of the injection chamber, diffuse radially outward along the inner wall of the solid extraction column, pass through the adsorbent packing layer in the solid extraction column perpendicular to the axis, enter the outer regulating chamber, and finally flow into the waste collection component, realizing the selective adsorption of the target anti-tumor drug component and the separation of impurities. At the same time, the viscosity acquisition component detects the viscosity of the raw material solvent in real time. The control system replenishes the regulating liquid in the injection chamber and the regulating chamber through the liquid replenishment component according to the viscosity data to optimize the extraction rheological conditions.

[0011] The above approach has the following beneficial effects:

[0012] 1. This solution uses an injection chamber that extends to the inner wall of the solid extraction column to allow liquid to exit, thus achieving a radial flow path for the solvent of the raw material to be extracted. This allows the solvent to pass through the solid extraction column along the shortest radial thickness of the packing layer, resulting in a flow resistance that is much lower than that of traditional axial flow. This effectively solves the problem of column bed blockage caused by abnormal viscosity of biological samples.

[0013] 2. This solution uses a viscosity acquisition component to detect the viscosity of the raw material solvent online, and the control system automatically drives the liquid addition component to perform dilution adjustment. High-viscosity samples can be directly introduced into the device without manual pre-dilution, reducing the probability of flow rate fluctuations and extraction failures caused by viscosity differences.

[0014] 3. This scheme combines radial flow driven by negative pressure with viscosity feedback adjustment, enabling samples from different individual sources to complete extraction under optimal rheological conditions, thereby improving the stability of recovery rates and operational consistency between batches.

[0015] Furthermore, the sleeve body and the fixed tube body are connected by a detachable sleeve connection.

[0016] Beneficial effects: This design allows operators to flexibly replace different types or specifications of solid extraction columns according to the detection requirements of different anti-tumor drugs, without having to replace the entire device, reducing operating costs, while ensuring the sealing reliability of the connection between the sleeve body and the fixed tube body.

[0017] Furthermore, the extraction column has a frustum-shaped structure at one end near the fixed tube, and a conical hole penetrating the extraction column is opened at the other end of the extraction column. Both the upper and lower surfaces of the extraction column are inclined frustum-shaped annular surfaces.

[0018] Beneficial effects: When the raw material solvent to be extracted flows radially outward along the solid extraction column, the actual flow path is downward along the inclined annular surface. Compared with the completely horizontal radial flow, it effectively suppresses local channeling or short-circuiting phenomena, making the sample more fully contacted with the packing bed and the path longer, thereby improving the dynamic adsorption capacity and packing utilization of the solid extraction column.

[0019] Furthermore, the waste collection assembly includes an annular waste collection cavity, which is sleeved and fixed on the outer wall of the sleeve body. The waste collection cavity is connected to the conical orifice solid extraction column, and the negative pressure drive component is connected to the waste collection cavity.

[0020] Beneficial effects: The waste collection chamber is connected to the conical-shaped solid extraction column, and the negative pressure drive component is directly connected to the waste collection chamber. This arrangement ensures that the negative pressure gradient is evenly distributed across the entire outer ring surface of the solid extraction column, driving the liquid to smoothly flow into the waste collection chamber after passing radially through the packing layer from the injection chamber. This reduces the flow field distortion caused by uneven local negative pressure, ensuring thorough waste liquid collection and flow stability.

[0021] Furthermore, the target component collection assembly includes an elution collection chamber fixedly connected to the bottom of the sleeve body. An elution port is provided at the bottom of the sleeve body corresponding to the shape of the solid extraction column. The elution collection chamber is connected to the inside of the sleeve body through the elution port.

[0022] When the negative pressure drive stops operating, the negative pressure guiding force from the injection chamber to the waste collection chamber disappears. The eluent injected through the liquid filling assembly will carry the target anti-tumor drug components adsorbed on the solid extraction column and enter the eluent collection chamber through the elution port.

[0023] Beneficial effects: When the negative pressure drive stops operating, the negative pressure guiding force from the injection chamber to the waste collection chamber disappears. At this time, the eluent injected through the liquid filling component carries the target antitumor drug component adsorbed on the solid extraction column under the action of gravity and enters the elution collection chamber through the elution port. This design realizes valveless switching between sample loading and elution modes by opening and closing the negative pressure, realizing the physical separation of waste liquid and target fraction and the isolation of liquid flow paths in different processes, reducing the risk of cross-contamination, and improving extraction purity and batch-to-batch reproducibility.

[0024] Furthermore, the liquid filling assembly includes a liquid filling box that can be detachably snapped and fixed to the top of the fixed tube. The liquid filling box includes a mixing chamber, a central injection channel, and an outer peripheral adjustment channel. Both the central injection channel and the outer peripheral adjustment channel are connected to the mixing chamber. An inlet pipe is connected to the top of the mixing chamber. The central injection channel is connected to the injection chamber. The outer peripheral adjustment channel is connected to the adjustment chamber. An adjustment liquid pipe is also connected to the side wall of the mixing chamber.

[0025] Beneficial effects: This dual-channel distribution structure allows the conditioning fluid (diluent or replenishment fluid) to be delivered independently to the injection chamber and the conditioning chamber, realizing zoned control of the inner and outer ring sides of the solid extraction column, providing a fluid path basis for subsequent viscosity adjustment and anti-drying compensation.

[0026] Furthermore, the viscosity acquisition component includes an acquisition tube, with both ends of the acquisition tube connected to the body of the liquid inlet tube. Differential pressure sensors are integrated on the inner walls of both ends of the acquisition tube. The differential pressure sensors are used to detect the pressure drop of the solvent of the raw material to be extracted flowing through the acquisition tube. The differential pressure sensors are connected to the control system signal.

[0027] Beneficial effects: This non-invasive detection method requires no additional sampling and does not interrupt the sample introduction process, and can obtain fluid viscosity data in real time, providing fast and accurate input parameters for the adaptive dilution adjustment of the control system.

[0028] Furthermore, a liquid level sensor is also provided at the bottom of the partition cylinder. The liquid level sensor is fixedly connected to the inner wall of the partition cylinder at the height corresponding to the top of the liquid outlet, and the liquid level sensor is connected to the control system signal.

[0029] Beneficial effects: The liquid level sensor is located on the inner wall of the diaphragm at the height of the top of the outlet, and is used to monitor the liquid level in the injection chamber in real time and transmit the signal to the control system. This arrangement can capture the time point when the liquid level drops to the critical position, providing a trigger condition for active anti-drying compensation and reducing the probability of local drying of the solid extraction column packing layer due to low liquid level.

[0030] Furthermore, the control system includes a liquid level monitoring and control module;

[0031] The liquid level monitoring and control module is used to receive the liquid level height data in the injection chamber collected by the liquid level sensor. The liquid level monitoring and control module has a preset liquid level safety threshold. When the liquid level height data is lower than the liquid level safety threshold, the output terminal of the liquid level monitoring and control module outputs a first control signal to the regulating liquid pipe to control the regulating liquid pipe to fill the mixing chamber with a preset volume of regulating liquid.

[0032] After the conditioning solution is split in the mixing chamber, part of it enters the injection chamber through the central injection channel and wets the inner ring sidewall of the solid extraction column from the outlet upwards, while the other part enters the conditioning chamber through the peripheral conditioning channel and wets the outer ring sidewall of the solid extraction column from the outside to the inside in the opposite direction.

[0033] Beneficial effects: This design maintains full wetting of the entire annular packing layer of the solid extraction column under continuous negative pressure suction through a bidirectional synchronous replenishment mechanism, eliminating the risk of column bed drying; at the same time, the reverse liquid flow effectively suppresses the local overrush effect caused by uneven radial flow velocity, realizing the homogenization retention and mass transfer of the target components in the packing layer.

[0034] Furthermore, the control system also includes a viscosity adaptive adjustment module;

[0035] The viscosity adaptive control module is used to receive pressure drop data from both ends of the acquisition tube collected by the differential pressure sensor, and calculate the real-time viscosity value of the solvent of the raw material to be extracted based on the pressure drop data.

[0036] The viscosity adaptive control module has a preset upper limit threshold and a viscosity target threshold. When the real-time viscosity value is higher than the upper limit threshold, the viscosity adaptive control module outputs an instruction to fill the mixing chamber with dilution control liquid through the control liquid pipe.

[0037] Driven by a negative pressure drive, the dilution and conditioning liquid is pre-mixed and diluted with the solvent of the raw material to be extracted in the mixing chamber, and then enters the injection chamber through the central injection channel.

[0038] Beneficial effects: The viscosity adaptive control module enables online real-time detection and adaptive dilution adjustment of the raw material solvent viscosity, allowing high-viscosity samples to be directly introduced into the device without manual pre-dilution. This reduces the risk of column blockage while maintaining the stability of radial penetration flow rate, and improves the device's adaptability to biological samples from different individuals.

[0039] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0040] Figure 1This is a schematic diagram of the overall structure of an embodiment of the extraction device for detecting antitumor drug components of the present invention;

[0041] Figure 2 This is an isometric sectional view illustrating the installation of the fixed tube and the sleeve in an embodiment of the extraction device for detecting antitumor drug components of the present invention;

[0042] Figure 3 This is an isometric sectional view of the interior of the fixed tube and the sleeve in an embodiment of the extraction device for detecting antitumor drug components of the present invention;

[0043] Figure 4 for Figure 3 A partially enlarged view of the liquid level sensor arrangement at point A in the middle;

[0044] Figure 5 This is an axonometric sectional view of the solid extraction column in an embodiment of the extraction device for detecting antitumor drug components of the present invention.

[0045] The reference numerals in the accompanying drawings of the instruction manual include: 1. Fixed tube body; 101. Injection chamber; 101a. Outlet; 102. Adjustment chamber; 2. Divider; 3. Tube body; 4. Solid extraction column; 5. Waste collection chamber; 6. Negative pressure drive component; 7. Elution collection chamber; 8. Elution port; 9. Liquid filling assembly; 901. Liquid filling box; 902. Mixing chamber; 903. Central injection channel; 904. Peripheral adjustment channel; 905. Inlet pipe; 906. Adjustment liquid pipe; 10. Viscosity acquisition assembly; 11. Liquid level sensor. Detailed Implementation

[0046] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0047] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0048] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0049] The following detailed description illustrates the specific implementation method:

[0050] Example 1:

[0051] This embodiment provides an extraction device for detecting antitumor drug components, specifically as follows: Figures 1-5 As shown, it includes a fixed tube body 1, and a partition cylinder 2 is coaxially fixedly connected inside the fixed tube body 1. The partition cylinder 2 divides the interior of the fixed tube body 1 into an injection chamber 101 and an adjustment chamber 102. The inner injection chamber 101 is used to carry the raw material solvent to be extracted and transport it. The outer adjustment chamber 102 has an annular structure.

[0052] The bottom of the fixed tube 1 is tightly connected to the sleeve 3 via a detachable method (preferably a snap-fit ​​connection). A solid extraction column 4 is placed inside the sleeve 3; one end of the solid extraction column 4 near the fixed tube 1 is a frustum-shaped structure, and the other end of the solid extraction column 4 has a conical hole penetrating the column. Both the upper and lower surfaces of the solid extraction column 4 are inclined frustum-shaped annular surfaces (within a certain range). Figure 5 The structure shown is a hollow annular structure formed by rotating a parallelogram-shaped cross-section around the central axis of the sleeve body 3. Both the upper and lower surfaces of the extraction column 4 are inclined frustum-shaped annular surfaces. The interior of the extraction column 4 is filled with adsorbent packing material (not shown in the attached diagram, using silica-based bonded phase adsorbent, i.e., with a particle size of 40-60 μm) for selective adsorption of target antitumor components. (Taking the extraction of aromatic ring structure drugs using μm spherical silica matrix adsorbent packing as an example); correspondingly, the bottom end of the injection cavity 101 extends to the inner ring wall of the solid extraction column 4 (i.e., the injection cavity 101 extends downwards, and the bottom end abuts and adheres to the inner ring wall of the solid extraction column 4), and several uniformly arranged liquid outlets 101a are opened at the bottom of the injection cavity 101 corresponding to the position of the solid extraction column 4; compared with the traditional technology in which the solvent to be extracted flows through the solid extraction column 4 along the axial direction by gravity to achieve solid phase extraction, in this embodiment the solvent to be extracted will enter through several uniformly arranged liquid outlets 101a at the bottom of the injection cavity 101 and diffuse radially outward along the inner ring wall of the solid extraction column 4, and pass horizontally through the adsorbent packing inside it in a direction perpendicular to the central axis of the solid extraction column 4, so as to realize the rapid permeation extraction of the solvent from the inside out along the shortest path (radial thickness) of the adsorbent packing, reduce the flow resistance of the sample and the risk of blockage of the solid extraction column 4, and improve the extraction efficiency.

[0053] Furthermore, compared to the horizontal sieve plate and packing combined with the sieve plate sandwich structure in traditional filtration technology, the annular frustum-shaped solid extraction column 4 in this embodiment takes into account the influence of gravity on the permeation of the solvent to be extracted. When the solvent to be extracted flows outward along the radial direction of the solid extraction column 4, its actual flow path is downward along the inclined annular surface, reducing the local channeling or short-circuiting that may occur in horizontal flow, making the contact between the sample and the packing bed more sufficient and the path longer, thereby improving the dynamic adsorption capacity and utilization rate of the solid extraction column 4.

[0054] The casing 3 integrates a waste collection component and a target component collection component; such as Figure 3 As shown, the waste collection assembly includes an annular waste collection chamber 5, which is sleeved and fixed on the outer wall of the sleeve body 3. The waste collection chamber 5 is connected to the conical hole (inside the annulus of the solid extraction column 4). In particular, the bottom of the waste collection chamber 5 is also connected to a negative pressure drive 6 (preferably a micro vacuum pump, as a negative pressure gradient source). The negative pressure drive 6 is signal-connected to the control system. When the operator adds the raw material solvent to be extracted (especially clinical biological samples, such as plasma) into the injection chamber 101, the control system activates the negative pressure drive 6. Between the injection chamber 101 (at normal pressure) and the waste collection chamber 5 (negative pressure), a radial negative pressure gradient is established through the solid extraction column 4 from the injection chamber 101 to the waste collection chamber 5. The flow of the raw material solvent to be extracted along the radial diffusion of the solid extraction column 4 is driven by the radial negative pressure gradient. At this time, the target anti-tumor drug component will be selectively adsorbed onto the adsorbent packing, while the unadsorbed liquid phase components such as water, salt, and polar interfering substances will penetrate the solid extraction column 4 and flow into the outer waste collection chamber 5 for collection, thus completing the sample loading and impurity removal.

[0055] The bottom of the sleeve body 3 is equipped with a target component collection assembly, which includes an elution collection chamber 7 fixedly connected to the bottom of the sleeve body 3. The bottom of the sleeve body 3 has an elution port 8 corresponding to the shape of the solid extraction column 4. The elution collection chamber 7 is connected to the inside of the sleeve body 3 through the elution port 8. When the solvent of the raw material to be extracted passes through the radial permeation of the solid extraction column 4 into the waste collection chamber 5, the negative pressure drive 6 stops operating, and the negative pressure guiding effect in the injection chamber 101 disappears. The operator can then inject the eluent into the regulating chamber 102. The eluent flows along the axial direction of the tube body or from the injection chamber 101. When the eluent passes through the solid extraction column 4, due to the combined effect of gravity and the stopped negative pressure, the eluent no longer enters the waste collection chamber 5 after passing through the solid extraction column 4. Instead, it carries the eluted target antitumor drug components and flows into the eluent collection chamber 7 through the elution port 8. The opening and closing of the negative pressure realizes the mode switching between elution and sample loading, achieves valveless physical separation of waste liquid and target fraction, and isolates the liquid flow path between different operation processes. This reduces the risk of cross-contamination of antitumor drug extract components caused by residues inside the solid extraction column 4, and improves the purity and batch-to-batch reproducibility of the extracted antitumor drug components.

[0056] This embodiment also includes a liquid filling assembly 9 on the fixed tube body 1. The liquid filling assembly 9 includes a liquid filling box 901 that is detachably snapped onto the top of the fixed tube body 1. The liquid filling box 901 includes a mixing chamber 902, a central liquid injection channel 903, and an outer peripheral adjustment channel 904. The central liquid injection channel 903 and the outer peripheral adjustment channel 904 are both connected to the mixing chamber 902. The top of the mixing chamber 902 is connected to an inlet pipe 905 (the inlet pipe 905 introduces the solvent of the raw material to be extracted into the mixing chamber 902). The central liquid injection channel 903 is connected to the injection chamber 101. The outer peripheral adjustment channel 904 is connected to the injection chamber 101. 04 is connected to the regulating chamber 102, and the side wall of the mixing chamber 902 is also connected to the regulating liquid pipe 906 (which can introduce diluent or regulating liquid as needed); correspondingly, the top of the injection chamber 101 is connected to the viscosity acquisition component 10, which includes an acquisition tube (not shown in the figure). The two ends of the acquisition tube are respectively connected to the tube body of the liquid inlet pipe 905. The inner tube wall at both ends of the acquisition tube is integrated with a differential pressure sensor. The differential pressure sensor is used to detect the pressure drop of the solvent of the raw material to be extracted flowing through the acquisition tube. The differential pressure sensor is connected to the control system signal.

[0057] Traditional SPEs often fail due to extremely slow flow rates or excessively high column pressures. This embodiment achieves viscosity detection through the aforementioned structure, i.e., viscosity pretreatment of different raw material solvents before solid-phase extraction. Specifically: a differential pressure sensor captures the pressure drop of the fluid flowing in the inlet pipe 905 in real time, and the control system receives the pressure drop data and calculates the real-time viscosity value. Once the real-time viscosity value exceeds the preset upper limit threshold, the control system immediately issues an instruction to precisely inject a certain volume of diluent (such as phosphate buffer or an organic solvent of appropriate concentration) into the mixing chamber 902 through the regulating pipe 906. The diluent is pre-mixed with the high-viscosity raw material solvent in the mixing chamber 902 to reduce its overall viscosity. This achieves viscosity correction before the sample enters the solid-phase extraction column 4, ensuring that subsequent radial extraction can be carried out under optimal rheological conditions, further reducing the probability of high-viscosity raw material solvent clogging the solid-phase extraction column 4.

[0058] Example 2:

[0059] As solid-phase extraction proceeds, the liquid level in injection chamber 101 will continue to drop. When large-volume sample loading or slow rinsing is performed on the solid extraction column 4 for a long time, as the liquid level in injection chamber 101 decreases, relying solely on the liquid supply from the radial outlet 101a may cause localized drying or solvation layer rupture in the upper inner region of the annular packing layer of the solid extraction column 4 due to liquid flow shear. Once the solvation layer on the packing surface is lost, bubbles enter the micropores, which not only reduces the extraction efficiency but also makes subsequent rewetting extremely difficult.

[0060] Therefore, as attached Figure 3 and Figure 4As shown, the difference from Embodiment 1 is that, in this embodiment, a liquid level sensor 11 is also provided at the bottom of the partition cylinder 2. The liquid level sensor 11 is fixedly connected to the inner wall of the partition cylinder 2 at the height corresponding to the top of the liquid outlet 101a. The liquid level sensor 11 is connected to the control system signal. Correspondingly, the control system includes a liquid level monitoring and control module.

[0061] The liquid level monitoring and control module is used to receive the liquid level height data in the injection chamber 101 collected by the liquid level sensor 11. The liquid level monitoring and control module has a preset liquid level safety threshold. When the liquid level height data is lower than the liquid level safety threshold, the output end of the liquid level monitoring and control module outputs a first control signal to the regulating liquid pipe 906 to control the regulating liquid pipe 906 to fill the mixing chamber 902 with a preset volume of regulating liquid. After the regulating liquid is diverted through the mixing chamber 902, part of it enters the injection chamber 101 through the central injection channel 903 and wets the inner ring sidewall of the solid extraction column 4 upward from the liquid outlet 101a. The other part enters the regulating chamber 102 through the outer peripheral regulating channel 904 and wets the outer ring sidewall of the solid extraction column 4 in the reverse direction from the outside to the inside.

[0062] By employing a reverse replenishment mechanism, the entire annular packing layer of the extraction column 4 (upper, middle, and lower regions) is fully wetted under continuous negative pressure suction, reducing the probability of the extraction column bed drying out. At the same time, the reverse liquid flow is used to suppress the local overshoot effect caused by uneven radial flow rate, thereby achieving homogenization retention and mass transfer of the target components within the packing layer.

[0063] Example 3:

[0064] The difference from Example 2 is that the control system also includes a viscosity adaptive adjustment module.

[0065] The viscosity adaptive control module receives pressure drop data from both ends of the acquisition tube collected by the differential pressure sensor, and calculates the real-time viscosity value of the solvent to be extracted based on the pressure drop data. The viscosity adaptive control module has preset upper limit threshold and viscosity target threshold. When the real-time viscosity value is higher than the upper limit threshold, the viscosity adaptive control module outputs an instruction to fill the mixing chamber 902 with diluent through the regulating liquid tube 906. Driven by the negative pressure drive 6, the diluent is pre-mixed and diluted with the solvent to be extracted in the mixing chamber 902, and then enters the injection chamber 101 through the central injection channel 903. This realizes online real-time detection and adaptive dilution adjustment of the solvent viscosity, allowing high-viscosity samples to be directly introduced into the device without manual pre-dilution, reducing the risk of column blockage while maintaining the stability of the radial penetration flow rate.

[0066] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. An extraction device for detecting antitumor drug components, comprising a fixed tube (1), characterized in that, A coaxially arranged partition (2) is fixedly connected inside the fixed tube (1). The partition (2) divides the inside of the fixed tube (1) into an inner injection chamber (101) and an outer ring-shaped adjustment chamber (102). The bottom of the fixed tube (1) is provided with a sleeve (3), and a solid extraction column (4) with a frustum ring structure is placed inside the sleeve (3). The solid extraction column (4) is filled with adsorbent packing material. The sleeve (3) is provided with a waste collection component and a target component collection component. The waste collection component is used to collect the liquid phase components that are not adsorbed after passing through the solid extraction column (4), and the target component collection component is used to collect the liquid phase components containing the target anti-tumor drug components. The bottom end of the injection chamber (101) extends to the inner wall of the solid extraction column (4). Several liquid outlets (101a) are opened at the bottom of the injection chamber (101) corresponding to the position of the solid extraction column (4). The waste collection component is also connected to a negative pressure drive (6). The negative pressure drive (6) is connected to a control system. When the negative pressure drive (6) is activated, a negative pressure gradient is formed along the regulating chamber (102) to the waste collection component, driving the solvent of the raw material to be extracted to pass through the solid extraction column (4) radially from the injection chamber (101) and enter the waste collection component. The top of the injection chamber (101) is connected to a viscosity acquisition component (10) for collecting viscosity data of the solvent of the raw material to be extracted. The fixed tube (1) is provided with a liquid filling component (9) for adjusting the viscosity of the solvent of the raw material to be extracted. The injection chamber (101) and the adjustment chamber (102) are both connected to the liquid filling component (9). The viscosity acquisition component (10) and the liquid filling component (9) are both connected to the control system signal.

2. The extraction device for detecting antitumor drug components according to claim 1, characterized in that, The sleeve body (3) and the fixed tube body (1) are connected by a detachable sleeve.

3. The extraction device for detecting antitumor drug components according to claim 2, characterized in that, The solid extraction column (4) has a frustum-shaped structure at one end near the fixed tube (1), and a conical hole is provided at the other end of the solid extraction column (4) to pass through the solid extraction column (4). The upper and lower surfaces of the solid extraction column (4) are both inclined frustum-shaped annular surfaces.

4. The extraction device for detecting antitumor drug components according to claim 3, characterized in that, The waste collection assembly includes an annular waste collection cavity (5), which is sleeved and fixed on the outer wall of the sleeve body (3). The waste collection cavity (5) is connected to the conical hole of the solid extraction column, and the negative pressure drive (6) is connected to the waste collection cavity (5).

5. The extraction device for detecting antitumor drug components according to claim 4, characterized in that, The target component collection assembly includes an elution collection chamber (7) fixedly connected to the bottom of the sleeve body (3). The bottom of the sleeve body (3) is provided with an elution port (8) corresponding to the shape of the solid extraction column (4). The elution collection chamber (7) is connected to the inside of the sleeve body (3) through the elution port (8). When the negative pressure drive (6) stops operating, the negative pressure guiding force from the injection chamber (101) to the waste collection chamber (5) disappears. The eluent injected through the liquid filling assembly (9) will carry the target anti-tumor drug components adsorbed on the solid extraction column (4) and enter the eluent collection chamber (7) through the eluent port (8).

6. The extraction device for detecting antitumor drug components according to claim 5, characterized in that, The liquid filling assembly (9) includes a liquid filling box (901) that can be detachably snapped and fixed to the top of the fixed tube body (1). The liquid filling box (901) includes a mixing chamber (902), a central liquid injection channel (903), and an outer peripheral adjustment channel (904). The central liquid injection channel (903) and the outer peripheral adjustment channel (904) are both connected to the mixing chamber (902). The top of the mixing chamber (902) is connected to an inlet pipe (905). The central liquid injection channel (903) is connected to the injection chamber (101). The outer peripheral adjustment channel (904) is connected to the adjustment chamber (102). An adjustment liquid pipe (906) is also connected to the side wall of the mixing chamber (902).

7. The extraction device for detecting antitumor drug components according to claim 6, characterized in that, The viscosity acquisition component (10) includes an acquisition tube, with both ends of the acquisition tube connected to the body of the liquid inlet tube (905). The inner walls of both ends of the acquisition tube are integrated with differential pressure sensors. The differential pressure sensors are used to detect the pressure drop of the solvent of the raw material to be extracted flowing through the acquisition tube. The differential pressure sensors are connected to the control system signal.

8. The extraction device for detecting antitumor drug components according to claim 7, characterized in that, A liquid level sensor (11) is also provided at the bottom of the partition (2). The liquid level sensor (11) is fixedly connected to the inner wall of the partition (2) at the height corresponding to the top of the liquid outlet (101a). The liquid level sensor (11) is connected to the control system signal.

9. The extraction device for detecting antitumor drug components according to claim 8, characterized in that, The control system includes a liquid level monitoring and control module; The liquid level monitoring and control module is used to receive the liquid level height data in the injection chamber (101) collected by the liquid level sensor (11); the liquid level monitoring and control module has a preset liquid level safety threshold. When the liquid level height data is lower than the liquid level safety threshold, the output end of the liquid level monitoring and control module outputs a first control signal to the regulating liquid pipe (906) to control the regulating liquid pipe (906) to fill the mixing chamber (902) with a preset volume of regulating liquid; After the conditioning liquid is diverted through the mixing chamber (902), a portion enters the injection chamber (101) through the central injection channel (903) and wets the inner ring sidewall of the solid extraction column (4) upward from the outlet (101a). The other portion enters the conditioning chamber (102) through the peripheral conditioning channel (904) and wets the outer ring sidewall of the solid extraction column (4) in the reverse direction from the outside to the inside.

10. The extraction device for detecting antitumor drug components according to claim 9, characterized in that, The control system also includes a viscosity adaptive adjustment module; The viscosity adaptive control module is used to receive pressure drop data from both ends of the acquisition tube collected by the differential pressure sensor, and calculate the real-time viscosity value of the solvent of the raw material to be extracted based on the pressure drop data. The viscosity adaptive control module has a preset upper limit threshold and a viscosity target threshold. When the real-time viscosity value is higher than the upper limit threshold, the viscosity adaptive control module outputs an instruction to fill the mixing chamber (902) with dilution control liquid through the control liquid pipe (906). Driven by the negative pressure drive (6), the dilution conditioning liquid is pre-mixed and diluted with the solvent of the raw material to be extracted in the mixing chamber (902), and then enters the injection chamber (101) through the central injection channel (903).

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