A double-wax block synchronous slicing assembly and a double-wax block synchronous slicing method

Abstract

This invention provides a dual-paraffin block simultaneous sectioning assembly and method. The dual-paraffin block simultaneous sectioning assembly includes a tray, a reference plate, an embedding cassette assembly, and a clamping connector. During sectioning, the tissue embedding cassette is placed on the tray and pressed down to ensure the tissue sample paraffin block is in contact with the reference plate. Next, a control sample paraffin block is placed on the reference plate, with one end aligned with the tissue sample paraffin block. The control embedding cassette is then installed on one side of the tissue embedding cassette. Paraffin is then injected into the control embedding cassette to bond it to the control sample paraffin block. After the paraffin solution cools, the tissue embedding cassette and the control embedding cassette are removed and installed in the clamping opening of the clamping connector, allowing for sectioning. This dual-paraffin block simultaneous sectioning assembly enables simultaneous sectioning of both tissue and control sample paraffin blocks in one operation, simplifying the slide preparation process, reducing the burden of repetitive operations for pathologists, and significantly improving work efficiency.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, specifically to a dual-wax block synchronous slicing assembly and a dual-wax block synchronous slicing method. Background Technology

[0002] Immunohistochemistry (IHC) is an indispensable core technique in clinical pathological diagnosis. Its principle is based on the specific binding of labeled antibodies to target antigens within tissue cells, followed by a colorimetric reaction that visually reveals the antigen's expression location and level. Because different lesions and tumor types have characteristic antigen expression patterns, pathologists can obtain crucial diagnostic information by interpreting the staining results, providing a core basis for determining the nature of the lesion, tumor classification, and precision treatment.

[0003] Currently, fully automated immunohistochemical staining instruments are widely used in clinical practice. While they significantly improve staining efficiency, the staining process is completed within the instrument's closed chamber, making real-time monitoring impossible for operators. Occasional abnormalities such as antibody slippage, tissue detachment, and air bubbles on the slide are difficult to detect promptly, potentially leading to partial or complete staining failure and directly impacting the accuracy of pathological diagnoses. To avoid diagnostic biases caused by staining failure, immunohistochemical testing routinely includes positive control tissues. The staining results of these control tissues verify the reliability of the staining procedure. Specifically, positive control tissues must show a clear positive staining result after staining; this is the gold standard for determining the effectiveness of the staining procedure. Conversely, if the control tissue does not show a positive result, the staining is considered a failure, and the section must be stained again. (See clinical routine procedures for reference.) Figure 17-22 This method requires first sectioning and mounting the positive control paraffin block onto a glass slide, then transferring the tissue section to the same slide. This ensures the positive control and the tissue are on the same slide, operating under identical staining conditions throughout the process, thus guaranteeing accurate verification of the staining procedure's effectiveness. However, this method necessitates two separate sectioning and retrieval operations. For large tertiary hospitals processing 600-3000 slides daily, each slide requires separate preparation of both the control and the tissue to be examined, nearly doubling the workload and placing a significant operational burden on the sectioning staff.

[0004] Therefore, how to design a slide preparation component that can simplify the slide preparation process and reduce the operational burden on pathology technicians is a technical problem that has not yet been solved in the current technology. Summary of the Invention

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the technical defects of the prior art in which the preparation of control tissue and test tissue must be completed separately for each single slide, which nearly doubles the workload and brings a significant operational burden to the slide preparation position. The present invention provides a dual-paraffin block synchronous slide preparation component that can simplify the slide preparation process and reduce the operational burden of pathology technicians.

[0006] This invention also provides a method for simultaneous slicing of two wax blocks.

[0007] Therefore, the present invention provides a dual-wax block synchronous slicing assembly, comprising: The tray has an installation groove, and positioning groove edges are formed on both axial sides of the top opening of the installation groove. The outer side wall of the positioning groove edge protrudes upward to form a limiting stop edge. The reference plate can be installed vertically and vertically within the mounting slot; An embedding cassette assembly is removably placed on the sides of the positioning grooves on both sides of the mounting groove, located between the limiting stops on both sides; the embedding cassette assembly includes a tissue embedding cassette and a control embedding cassette, the tissue embedding cassette and the control embedding cassette are arranged side by side along the length of the tray and are detachably connected, the tissue embedding cassette is suitable for carrying tissue sample wax blocks, and the control embedding cassette is suitable for carrying control sample wax blocks; The clamping connector has a back end adapted for detachable mounting on a cryostat and a front end with a clamping opening; the embedding cassette assembly is detachably mounted within the clamping opening.

[0008] As a preferred embodiment, the tissue embedding cassette is formed with a wax injection groove, the wax injection groove having a top opening; a first mating bevel is formed on the outer wall of one side of the tissue embedding cassette, and a first mating plane is formed at the bottom of the first mating bevel; The control embedding cassette is elastically deformable, and a positioning plate is formed on one side facing the tissue embedding cassette. A connecting block is formed below the positioning plate. A second docking slope is formed on one side of the control embedding cassette, which can match and dock with the first docking slope. A second docking plane is formed at the bottom of the second docking slope, which can match and dock with the first docking plane. When the control embedding cassette is connected to the tissue embedding cassette, the connecting block is inserted into the wax injection groove through the top opening and pushes one side of the inner wall of the tissue embedding cassette, so that the first mating slope and the second mating slope are tightly fitted together, and the first mating plane and the second mating plane are tightly fitted together, thereby detachably connecting the tissue embedding cassette and the control embedding cassette together.

[0009] As a preferred embodiment, positioning opening slots are provided on opposite sides of the positioning grooves on both sides. The width of the positioning opening slots is greater than or equal to the width of the positioning plate, so that the positioning plate can be installed in the positioning opening slots to limit the position of the tissue embedding box and the control embedding box.

[0010] As a preferred embodiment, the device also includes a trimming blade for removing the wax block at the junction of the tissue sample wax block and the control sample wax block; the trimming blade includes an inverted triangular blade head and a grip handle fixedly connected to the inverted triangular blade head.

[0011] As a preferred embodiment, it also includes a suture wax injection mold, which is removably and sealingly covered on the tissue embedding box and the control embedding box for filling the wax injection gap formed after excision with wax; The joint wax injection mold includes: A flat plate with a wax injection opening in the middle; Two inclined plates are formed on the two side walls along the axial direction of the wax injection opening; Two stepped mounting plates are formed at both ends of the flat plate and are used to be placed on the outer walls of the tissue embedding box and the control embedding box on both sides. After the joint wax injection mold is placed in place, the wax injection opening is aligned with the wax injection notch, and wax is injected into the wax injection notch through the wax injection opening to fill and connect the joint.

[0012] As a preferred embodiment, the reference plate is vertically and flexibly installed in the mounting slot via a mounting structure; The mounting structure includes: There are two through holes, which are formed through the inner bottom wall of the mounting groove; There are two limiting guide shafts, one end of which is fixedly installed at the bottom of the reference plate, and the other end of which extends outward after passing through the mounting through hole, and the other end is provided with a threaded blind hole. Two elastic elements are installed in the mounting groove and respectively sleeved on the limiting guide shaft; Two mounting bolts are screwed onto the threaded blind hole; Two limiting washers are fitted onto the mounting bolts, with an outer diameter larger than the inner diameter of the mounting through hole.

[0013] As a preferred embodiment, the clamping connector includes: The main body is adapted to be detachably mounted on the cryostat, and protrusions are formed on both sides of the bottom, with a mounting position formed between the two protrusions; The movable gripper is movably mounted on the main body via a movable connection structure; the movable gripper includes an operating dial and a clamping block fixedly disposed at the bottom of the operating dial, the clamping block being located within the mounting position; A clamping frame is fixedly installed on the front of the main body, located above the clamping block; an adjustable clamping opening is formed between the clamping frame and the clamping block.

[0014] As a preferred embodiment, the active connection structure includes: There are two internal thread blind holes, which are respectively opened on both sides of the main body. The inner wall of the inner side of the internal thread blind hole is a smooth surface. There are two connecting studs, which are screwed into the internal thread blind holes respectively. The inner part of the connecting stud is a smooth cylindrical section. Two guide holes are respectively opened on the main body in the vertical direction. One end is connected to the internal thread blind hole, and the other end extends downward and passes through the clamping block. There are two shaft grooves, each formed at the bottom of the clamping block and communicating with the guide hole; There are two spring shafts, each installed in the shaft groove; There are two tension springs, which are respectively inserted into the guide hole. One end is hung on the smooth cylindrical section of the connecting stud, and the other end is hung on the spring shaft. Two threaded through holes are respectively opened through the two protrusions; There are two blind holes for the rotating shaft, which are respectively opened on both sides of the clamping block; Two rotating shaft bolts are screwed into the threaded through holes respectively, and the free end of the optical shaft extends into the blind hole of the rotating shaft and is rotatably connected to the blind hole of the rotating shaft, thereby movably mounting the movable jaw on the main body.

[0015] As a preferred embodiment, a positioning groove is formed in the middle of the front side of the main body in a vertical direction. The width of the positioning groove is greater than or equal to the width of the positioning plate, so that the positioning plate can be installed in the positioning groove to position the tissue embedding box and the control embedding box. and / or; The operating lever is movably installed within the positioning groove.

[0016] As a preferred embodiment, the back of the main body is formed with mounting protrusions, and the main body is adapted to be detachably mounted on the cryostat via the mounting protrusions.

[0017] This invention also provides a method for simultaneous sectioning of two wax blocks, comprising the following steps: Secure the clamping connector to the cryostat slicer; Place the tissue embedding cassette containing the tissue sample wax block between the limiting edges on both sides of the tray and press it down so that the tissue sample wax block is tightly attached to the upper surface of the reference plate; place the control sample wax block on the reference plate so that one end of it aligns with the tissue sample wax block; snap the control embedding cassette onto one side of the tissue embedding cassette and cover the control sample wax block; inject wax into the control embedding cassette to fix the control sample wax block to the control embedding cassette. Take out the tissue embedding cassette and the control embedding cassette together as a whole, flip them over and place them between the limiting edges on both sides of the tray, and install the positioning plate in the positioning opening slot; Using a trimming knife, a notch is formed at the connection between the tissue sample wax block and the control sample wax block. The suture wax injection mold is placed on the tissue embedding box and the control embedding box, with the wax injection opening facing the notch left after the excision. Wax is injected into the notch through the wax injection opening to fix the tissue sample wax block and the control sample wax block together. Move the operating lever to drive the clamping block to rotate downwards, expanding the space of the clamping opening. Place the tissue embedding box and the control embedding box, which are snapped together, into the clamping opening, so that the positioning plate is installed in the positioning groove. Release the operating lever, and the clamping block resets and cooperates with the clamping frame to clamp and fix the tissue embedding box and the control embedding box in the clamping opening. A cryostat was used to simultaneously section tissue sample blocks and control sample blocks that were fixed together.

[0018] The technical solution provided by this invention has the following advantages: 1. The dual-wax block synchronous sectioning assembly of the present invention includes a tray, a reference plate, an embedding cassette assembly, and a clamping connector; wherein, the tray has an installation groove, and positioning groove edges are formed on both axial sides of the top opening of the installation groove, and the outer side wall of the positioning groove edge protrudes upward to form a limiting stop edge; the reference plate is vertically and vertically installed in the installation groove; the embedding cassette assembly is removably placed on the positioning groove edges on both sides of the installation groove, located between the two limiting stops edges; the embedding cassette assembly includes a tissue embedding cassette and a control embedding cassette, the tissue embedding cassette and the control embedding cassette are arranged side by side along the length direction of the tray and are detachably connected, the tissue embedding cassette is suitable for carrying tissue sample wax blocks, and the control embedding cassette is suitable for carrying control sample wax blocks; the back of the clamping connector is suitable for detachable installation on a cryostat, and the front is provided with a clamping opening; the embedding cassette assembly is detachably installed in the clamping opening.

[0019] Before sectioning, secure the clamping connector to the cryostat. During sectioning preparation, first place the tissue embedding cassette containing the tissue sample paraffin block between the limiting edges on both sides of the tray and press it down so that the lower surface of the tissue sample paraffin block is in contact with the upper surface of the reference plate. Next, place the control sample paraffin block on the reference plate, aligning one end with the tissue sample paraffin block. Then, snap the control embedding cassette onto one side of the tissue embedding cassette, covering the control sample paraffin block. Next, inject wax into the control embedding cassette to bond it to the control sample paraffin block. After the wax has cooled, remove the connected tissue embedding cassette and control embedding cassette as a whole and install them into the clamping opening of the clamping connector. Sectioning can then be performed.

[0020] The dual-paraffin block synchronous sectioning assembly of this invention can simultaneously section tissue sample paraffin blocks and control sample paraffin blocks in one operation, fundamentally simplifying the slide preparation process, reducing the burden of repetitive operations for pathologists, and significantly improving work efficiency. At the same time, since the two sample paraffin blocks are cut simultaneously as a whole, the thickness and plane of the sections are completely consistent, thereby ensuring that the control sample paraffin blocks and tissue sample paraffin blocks are under completely consistent physical conditions in the subsequent immunohistochemical staining process. This further improves the reliability of the control results for verifying the staining process and provides more accurate technical support for pathological diagnosis.

[0021] 2. This invention also provides a method for simultaneous sectioning of two paraffin blocks. Compared with existing technologies, this method can simultaneously section tissue sample paraffin blocks and control sample paraffin blocks in one operation, fundamentally simplifying the slide preparation process, reducing the burden of repetitive operations for pathologists, and significantly improving work efficiency. Simultaneously, since the two sample paraffin blocks are cut simultaneously as a single unit, the thickness and plane of the sections are completely consistent, ensuring that the control sample paraffin block and the tissue sample paraffin block are under completely identical physical conditions during subsequent immunohistochemical staining. This further improves the reliability of the control results in verifying the staining process and provides more accurate technical support for pathological diagnosis. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the prior art or specific embodiments of the present invention, the accompanying drawings used in the description of the prior art or specific embodiments are briefly introduced below.

[0023] Figure 1 This is a schematic diagram of the overall structure of the dual wax block synchronous slicing assembly of the present invention.

[0024] Figure 2 yes Figure 1 A schematic diagram of the structure of the middle tray.

[0025] Figure 3 yes Figure 2A sectional view.

[0026] Figure 4 yes Figure 1 Schematic diagram of the structure of the tissue embedding cassette and the control embedding cassette.

[0027] Figure 5 yes Figure 4 Another stereoscopic view.

[0028] Figure 6 This is a schematic diagram of the structure after the tissue embedding cassette and the control embedding cassette are connected.

[0029] Figure 7 This is a schematic diagram of the wax block at the junction of the excised tissue sample wax block and the control sample wax block.

[0030] Figure 8 yes Figure 7 A schematic diagram of the structure of a dressing tool.

[0031] Figure 9 This is a schematic diagram of filling the wax injection gap formed after excision with wax.

[0032] Figure 10 yes Figure 9 A schematic diagram of the structure of the wax injection mold for the middle joint.

[0033] Figure 11 yes Figure 1 A schematic diagram of the clamping connector.

[0034] Figure 12 yes Figure 11 Another stereoscopic view.

[0035] Figure 13 yes Figure 11 A schematic diagram of the explosion structure.

[0036] Figure 14 yes Figure 11 A sectional view.

[0037] Figure 15 yes Figure 14 Enlarged structural diagram of part A.

[0038] Figure 16 yes Figure 11 Another stereoscopic view.

[0039] Figure 17 This is a schematic diagram of mounting a control wax block slice onto a glass slide.

[0040] Figure 18 This is a schematic diagram of using a microtome to slice a paraffin block of tissue.

[0041] Figure 19This is a schematic diagram of placing tissue paraffin blocks into water.

[0042] Figure 20 This is a diagram showing the placement of tissue paraffin slices in water.

[0043] Figure 21 This is a schematic diagram of retrieving tissue sections from a glass slide containing a control paraffin section.

[0044] Figure 22 This is a schematic diagram of control paraffin sections and tissue paraffin sections on the same glass slide.

[0045] Reference numerals: 1. Tray; 11. Mounting groove; 12. Positioning groove edge; 13. Limiting stop edge; 14. Positioning opening groove; 15. Identification notch; 2. Reference plate; 21. Mounting through hole; 22. Limiting guide shaft; 24. Elastic element; 25. Mounting bolt; 26. Limiting washer; 3. Tissue embedding cassette; 31. Wax injection tank; 32. First mating bevel; 33. First mating plane; 4. Reference embedding cassette; 41. Positioning plate; 42. Connecting block; 43. Second mating bevel; 44. Second mating plane; 5. Clamping connector; 51. Clamping opening; 5 2. Main body; 53. Protrusion; 54. Mounting position; 55. Movable gripper; 56. Operating dial; 57. Clamping block; 58. Clamping frame; 6. Trimming tool; 61. Inverted triangular cutter head; 62. Grip handle; 63. Joint wax injection mold; 64. Flat plate; 65. Wax injection opening; 66. Slanted plate; 67. Stepped mounting plate; 7. Internal thread blind hole; 71. Connecting stud; 72. Guide hole; 73. Shaft groove; 74. Hanging spring shaft; 75. Tension spring; 76. Threaded through hole; 77. Shaft blind hole; 78. Shaft bolt; 8. Positioning countersunk groove; 81. Mounting protrusion. Detailed Implementation

[0046] To enable those skilled in the art to better understand this solution, the technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of this application.

[0047] It should be noted that the terms "first," "second," etc., in the claims and specification of this application are used to distinguish similar objects and are not used to describe a specific order or sequence. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such as a process, method, system, product, or device that includes a series of steps or units, not limited to those steps or units explicitly listed, but may also include other steps or units not explicitly listed or inherent to these processes, methods, products, or devices.

[0048] In this application, the terms "upper," "lower," "left," "right," "front," "rear," "top," "bottom," "inner," "outer," "middle," "vertical," "horizontal," "lateral," and "longitudinal" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for better description of this application and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation. Furthermore, some of the above terms may be used to indicate other meanings besides orientation or positional relationship; for example, the term "upper" may also be used in some cases to indicate a certain dependency or connection relationship. Those skilled in the art can understand the specific meaning of these terms in this application according to the specific circumstances. In addition, the term "multiple" should mean two or more. It should be noted that, unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other.

[0049] The present application will now be described in detail with reference to the accompanying drawings and embodiments. Example 1

[0050] This embodiment provides a dual-wax block synchronous slicing assembly, such as... Figure 1-16 As shown, the assembly includes: a tray 1, a reference plate 2, an embedding cassette assembly, and a clamping connector 5. The tray 1 has a mounting groove 11, with positioning groove edges 12 formed on both axial sides of the top opening of the mounting groove 11. The outer side wall of the positioning groove edge 12 protrudes upward to form a limiting stop edge 13. The reference plate 2 is vertically and vertically mounted within the mounting groove 11. The embedding cassette assembly is removably placed on the positioning groove edges 12 on both sides of the mounting groove 11, located between the limiting stops 13 on both sides. The embedding cassette assembly includes a tissue embedding cassette 3 and a control embedding cassette 4, which are arranged side-by-side along the length of the tray 1 and are detachably connected. The tissue embedding cassette 3 is suitable for carrying tissue sample wax blocks, and the control embedding cassette 4 is suitable for carrying control sample wax blocks. The clamping connector 5 has a clamping opening 51 on its front side and is detachably mounted on its back side on a cryostat. The embedding cassette assembly is detachably mounted within the clamping opening 51.

[0051] Before sectioning, the clamping connector 5 is first fixedly installed on the cryostat. During sectioning preparation, the tissue embedding cassette 3, already containing the tissue sample wax block, is placed between the limiting edges 13 on both sides of the tray 1 and pressed downwards so that the lower surface of the tissue sample wax block is in contact with the upper surface of the reference plate 2. Next, the control sample wax block is placed on the reference plate 2, with one end aligned with the tissue sample wax block. Then, the control embedding cassette 4 is snapped onto one side of the tissue embedding cassette 3, covering the control sample wax block. Wax is then injected into the control embedding cassette 4 to bond it to the control sample wax block. After the wax solution cools, the tissue embedding cassette 3 and the control embedding cassette 4 are removed as a whole and installed in the clamping opening 51 of the clamping connector 5, ready for sectioning.

[0052] The dual-paraffin block synchronous sectioning assembly of this embodiment can simultaneously section tissue sample paraffin blocks and control sample paraffin blocks in one operation, fundamentally simplifying the slide preparation process, reducing the burden of repetitive operations for pathologists, and significantly improving work efficiency. At the same time, since the two sample paraffin blocks are cut simultaneously as a whole, the thickness and plane of the sections are completely consistent, thereby ensuring that the control sample paraffin blocks and tissue sample paraffin blocks are under completely consistent physical conditions in the subsequent immunohistochemical staining process. This further improves the reliability of the control results for verifying the staining process and provides more accurate technical support for pathological diagnosis.

[0053] like Figure 4-6 As shown, the tissue embedding cassette 3 has a wax injection groove 31 with a top opening; a first mating bevel 32 is formed on one side of the outer wall of the tissue embedding cassette 3, and a first mating plane 33 is formed at the bottom of the first mating bevel 32; the control embedding cassette 4 is elastically deformable, and a positioning plate 41 is formed on the side facing the tissue embedding cassette 3, with a connecting block 42 formed below the positioning plate 41; a second mating bevel 43 is formed on one side of the control embedding cassette 4 that can match and mate with the first mating bevel 32. The bottom of the two mating bevels 43 is formed with a second mating plane 44 that can match and mate with the first mating plane 33; when the control embedding box 4 is connected to the tissue embedding box 3, the connecting block 42 is inserted into the wax injection groove 31 through the top opening and pushes one side inner wall of the tissue embedding box 3 so that the first mating bevel 32 and the second mating bevel 43 fit tightly together, and the first mating plane 33 and the second mating plane 44 fit tightly together, thereby detachably connecting the tissue embedding box 3 and the control embedding box 4 together.

[0054] like Figure 2-3As shown, positioning opening slots 14 are provided opposite to each other on the two sides of the positioning groove edge 12. The width of the positioning opening slot 14 is greater than or equal to the width of the positioning plate 41, so that the positioning plate 41 can be installed in the positioning opening slot 14 to limit the tissue embedding box 3 and the control embedding box 4.

[0055] like Figure 7-8 As shown, it also includes a trimming blade 6, which is used to remove the wax block at the junction of the tissue sample wax block and the control sample wax block. The trimming blade 6 includes an inverted triangular blade head 61 and a grip handle 62 fixedly connected to the inverted triangular blade head 61. The operator uses the inverted triangular blade head 61 of the trimming blade 6 to cut along the junction of the tissue sample wax block and the control sample wax block; then, wax liquid is re-injected into the removed area. After the wax liquid cools and solidifies, the two can achieve a firm and smooth connection, thereby ensuring that the samples are on the same horizontal plane during subsequent sectioning. During the cutting process, the operator can cut a small identification notch 15 on the tissue sample wax block or the control sample wax block to facilitate the differentiation between the tissue sample wax block and the control sample wax block.

[0056] like Figure 9-10 As shown, it also includes a suture wax injection mold 63, which is removably and sealingly installed on the tissue embedding box 3 and the control embedding box 4 for filling the wax injection gap formed after excision with wax. The suture wax injection mold 63 includes a flat plate 64, an inclined plate 66, and a stepped mounting plate 67. The flat plate 64 has a wax injection opening 65 in the middle. There are two inclined plates 66, which are respectively formed on the two side walls of the wax injection opening 65 in the axial direction. There are two stepped mounting plates 67, which are respectively formed at both ends of the flat plate 64 for mounting on the outer side walls of the tissue embedding box 3 and the control embedding box 4. When the suture wax injection mold 63 is placed in place, the wax injection opening 65 is directly opposite the wax injection gap, and wax is injected into the wax injection gap through the wax injection opening 65 to fill and connect it. By injecting wax into the gap using the seam wax injection mold 63, it can be ensured that the surface of the refilled wax block is absolutely flush with the original wax block surfaces on both sides, thereby making the two wax blocks a single unit and on the same horizontal plane, ensuring the high consistency of the cutting plane between the tissue sample and the control sample during subsequent sectioning.

[0057] like Figure 3As shown, the reference plate 2 can be vertically and vertically installed in the mounting groove 11 via a mounting structure. The mounting structure includes mounting through holes 21, limiting guide shafts 22, elastic elements 24, mounting bolts 25, and limiting washers 26. There are two mounting through holes 21, which are formed through the inner bottom wall of the mounting groove 11. There are two limiting guide shafts 22, one end of which is fixedly disposed at the bottom of the reference plate 2, and the other end of which extends outward after passing through the mounting through holes 21, and the other end has a threaded blind hole. There are two elastic elements 24, which are respectively installed in the mounting groove 11 and respectively sleeved on the limiting guide shafts 22. There are two mounting bolts 25, which are screwed onto the threaded blind holes. There are two limiting washers 26, which are sleeved on the mounting bolts 25, and their outer diameter is larger than the inner diameter of the mounting through holes 21.

[0058] like Figure 11-12 As shown, the clamping connector 5 includes a main body 52, a movable jaw 55, and a clamping frame 58; wherein, the back of the main body 52 is adapted to be detachably mounted on the cryostat, and protrusions 53 are respectively formed on both sides of the bottom, forming a mounting position 54 between the two protrusions 53; the movable jaw 55 is movably mounted on the main body 52 through a movable connecting structure; the movable jaw 55 includes an operating dial 56 and a clamping block 57 fixedly disposed at the bottom of the operating dial 56, the clamping block 57 being located within the mounting position 54; the clamping frame 58 is fixedly mounted on the front of the main body 52, located above the clamping block 57; an adjustable clamping opening 51 is formed between the clamping frame 58 and the clamping block 57.

[0059] like Figure 13-16As shown, the movable connection structure includes an internally threaded blind hole 7, a connecting stud 71, a guide hole 72, a shaft groove 73, a hanging spring shaft 74, a tension spring 75, a threaded through hole 76, a rotating shaft blind hole 77, and a rotating shaft bolt 78; wherein, there are two internally threaded blind holes 7, respectively opened on both sides of the main body 52, and the inner wall of the inner side of the internally threaded blind hole 7 is a smooth surface; there are two connecting studs 71, respectively screwed into the internally threaded blind holes 7, and the inner side of the connecting studs 71 ​​is a smooth cylindrical section; there are two guide holes 72, respectively opened vertically on the main body 52, one end communicating with the internally threaded blind hole 7, and the other end extending downward and penetrating the clamping block 57; there are two shaft grooves 73, respectively opened on the... The bottom of the clamping block 57 is connected to the guide hole 72; there are two hanging spring shafts 74, which are respectively installed in the shaft groove 73; there are two tension springs 75, which are respectively inserted into the guide hole 72, with one end hanging on the smooth cylindrical section of the connecting stud 71 and the other end hanging on the hanging spring shaft 74; there are two threaded through holes 76, which are respectively opened through the two protrusions 53; there are two rotating shaft blind holes 77, which are respectively opened on both sides of the clamping block 57; there are two rotating shaft bolts 78, which are respectively screwed into the threaded through holes 76, and the free end of the optical shaft extends into the rotating shaft blind hole 77 and is rotatably connected to the rotating shaft blind hole 77, thereby movably installing the movable jaw 55 on the main body 52. When the tissue embedding cassette 3 and the control embedding cassette 4 need to be installed in the clamping opening 51, the operator moves the operating lever 56 towards themselves. At this time, the movable gripper 55 rotates around the pivot bolt 78, driving the bottom clamping block 57 to rotate downward in the installation position 54, thereby expanding the space of the clamping opening 51. During the downward rotation of the clamping block 57, the hanging spring shaft 74 moves downward synchronously, thereby stretching the tension spring 75 downward, so that the tension spring 75 is in a stretched and stored state. Then, the tissue embedding cassette 3 and the control embedding cassette 4 are placed in the clamping opening 51. After that, the operating lever 56 is released, and the tension spring 75, which is in a stretched and stored state, contracts and recovers, generating an upward elastic restoring force, pulling the hanging spring shaft 74 upward, and then driving the clamping block 57 to rotate upward and reset around the pivot bolt 78, cooperating with the clamping frame 58, thereby firmly clamping and fixing the tissue embedding cassette 3 and the control embedding cassette 4 in the clamping opening 51.

[0060] A positioning groove 8 is vertically formed in the center of the front of the main body 52. ​​The width of the positioning groove 8 is greater than or equal to the width of the positioning plate 41, allowing the positioning plate 41 to be installed within the positioning groove 8 to position the tissue embedding cassette 3 and the control embedding cassette 4. The operating lever 56 is movably installed within the positioning groove 8. Through the dual limiting and fixing of the clamping opening 51 and the positioning groove 8, the tissue embedding cassette 3 and the control embedding cassette 4 can be firmly fixed, effectively restricting their degrees of freedom in the horizontal and vertical directions, thereby ensuring that no displacement occurs during subsequent sectioning.

[0061] The back of the main body 52 is formed with a mounting protrusion 81, and the main body 52 is adapted to be detachably mounted on the cryostat via the mounting protrusion 81.

[0062] The method of using the dual-wax block synchronous sectioning assembly in this embodiment is as follows: Before sectioning, the clamping connector 5 is first fixedly installed on the cryostat. During sectioning preparation, the tissue embedding cassette 3, which already carries the tissue sample wax block, is placed between the limiting edges 13 on both sides of the tray 1 and pressed down so that the lower surface of the tissue sample wax block is in contact with the upper surface of the reference plate 2; then, the control sample wax block is placed on the reference plate 2, with one end of it aligned with the tissue sample wax block, and then the control embedding cassette 4 is snapped onto one side of the tissue embedding cassette 3, covering the top of the control sample wax block; then, wax is injected into the control embedding cassette 4 to bond the control embedding cassette 4 to the control sample wax block; After the wax liquid cools down, the tissue embedding cassette 3 and the control embedding cassette 4 connected together are taken out as a whole, flipped and placed between the limiting edges 13 on both sides of the tray 1, and the positioning plate 41 is installed in the positioning opening groove 14. The operator uses trimming knife 6 to cut along the connection between the tissue sample wax block and the control sample wax block; then the joint wax injection mold 63 is placed on the tissue embedding box 3 and the control embedding box 4, so that the wax injection opening 65 is directly opposite the notch left after the excision. Then wax is injected into the notch through the wax injection opening 65, so that the two wax blocks are connected as one and on the same horizontal plane. During slicing, the operator moves the operating lever 56 toward themselves, driving the clamping block 57 to rotate downwards and expand the space of the clamping opening 51. Then, the tissue embedding cassette 3 and the control embedding cassette 4 are placed in the clamping opening 51, so that the positioning plate 41 is installed in the positioning groove 8. After that, the operating lever 56 is released, and the clamping block 57 resets and cooperates with the clamping frame 58 to firmly clamp and fix the tissue embedding cassette 3 and the control embedding cassette 4 in the clamping opening 51, and the slicing operation can then be performed. Example 2

[0063] This embodiment provides a method for simultaneous sectioning of two wax blocks, referencing... Figure 1 ,6 As shown in 7 and 9, the steps include the following: Securely install the clamping connector 5 onto the cryostat; place the tissue embedding cassette 3, containing the tissue sample wax block, between the limiting edges 13 on both sides of the tray 1 and press it downwards so that the tissue sample wax block is tightly attached to the upper surface of the reference plate 2; place the control sample wax block on the reference plate 2, with one end aligned with the tissue sample wax block, and snap-fit ​​the control embedding cassette 4 onto one side of the tissue embedding cassette 3, covering the control sample wax block; inject wax into the control embedding cassette 4 to fix the control sample wax block to the control embedding cassette 4; remove the tissue embedding cassette 3 and the control embedding cassette 4 together as a whole, flip them over and place them between the limiting edges 13 on both sides of the tray 1, and install the positioning plate 41 in the positioning opening slot 14; use the trimming blade 6 to cut along the connection between the tissue sample wax block and the control sample wax block. A notch is formed, and the suture wax injection mold 63 is placed over the tissue embedding cassette 3 and the control embedding cassette 4, with the wax injection opening 65 facing the notch left after excision. Wax is injected into the notch through the wax injection opening 65 to fix the tissue sample wax block and the control sample wax block together. The operating dial 56 is turned to drive the clamping block 57 to rotate downward, expanding the space of the clamping opening 51. The tissue embedding cassette 3 and the control embedding cassette 4, which are clamped together, are placed in the clamping opening 51, so that the positioning plate 41 is installed in the positioning groove 8. The operating dial 56 is released, and the clamping block 57 returns to its original position and cooperates with the clamping frame 58 to clamp and fix the tissue embedding cassette 3 and the control embedding cassette 4 in the clamping opening 51. The tissue sample wax block and the control sample wax block, which are fixed together, are sectioned simultaneously using a cryostat.

[0064] Compared with existing technologies, the slicing method of this embodiment can simultaneously slice tissue sample paraffin blocks and control sample paraffin blocks in one operation, fundamentally simplifying the slide preparation process, reducing the burden of repetitive operations for pathologists, and significantly improving work efficiency. At the same time, since the two sample paraffin blocks are cut together simultaneously, the thickness and plane of the slices are completely consistent, thus ensuring that the control sample paraffin blocks and tissue sample paraffin blocks are under completely consistent physical conditions in the subsequent immunohistochemical staining process. This further improves the reliability of the control results for verifying the staining process and provides more accurate technical support for pathological diagnosis.

[0065] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the protection scope of this innovative technical solution.

Claims

1. A dual-wax block synchronous slicing assembly, characterized in that, include: The tray (1) has an installation groove (11). The top opening of the installation groove (11) has positioning groove edges (12) formed on both sides. The outer side wall of the positioning groove edge (12) protrudes upward to form a limiting stop edge (13). The reference plate (2) can be installed vertically in the mounting slot (11); The embedding cassette assembly is removably placed on the positioning groove edges (12) on both sides of the mounting groove (11), located between the limiting edges (13) on both sides; the embedding cassette assembly includes a tissue embedding cassette (3) and a control embedding cassette (4), the tissue embedding cassette (3) and the control embedding cassette (4) are arranged side by side along the length direction of the tray (1) and are detachably connected, the tissue embedding cassette (3) is suitable for carrying tissue sample wax blocks, and the control embedding cassette (4) is suitable for carrying control sample wax blocks; The clamping connector (5) is adapted to be detachably mounted on a cryostat and has a clamping opening (51) on the front; the embedding cassette assembly is detachably mounted in the clamping opening (51).

2. The dual-wax block synchronous slicing assembly according to claim 1, characterized in that: The tissue embedding box (3) is formed with a wax injection groove (31), the wax injection groove (31) has a top opening; a first docking slope (32) is formed on the outer wall of one side of the tissue embedding box (3), and a first docking plane (33) is formed at the bottom of the first docking slope (32). The control embedding cassette (4) is elastically deformable, and a positioning plate (41) is formed on one side facing the tissue embedding cassette (3). A connecting block (42) is formed below the positioning plate (41). A second docking slope (43) that can match and dock with the first docking slope (32) is formed on one side of the control embedding cassette (4). A second docking plane (44) that can match and dock with the first docking plane (33) is formed at the bottom of the second docking slope (43). When the control embedding box (4) is connected to the tissue embedding box (3), the connecting block (42) is inserted into the wax injection groove (31) through the top opening and pushes one side of the inner wall of the tissue embedding box (3) so that the first mating slope (32) and the second mating slope (43) are tightly fitted together, and the first mating plane (33) and the second mating plane (44) are tightly fitted together, thereby detachably connecting the tissue embedding box (3) and the control embedding box (4).

3. The dual-wax block synchronous slicing assembly according to claim 2, characterized in that: Positioning opening slots (14) are provided opposite to each other on the sides of the positioning groove (12). The width of the positioning opening slot (14) is greater than or equal to the width of the positioning plate (41), so that the positioning plate (41) can be installed in the positioning opening slot (14) to limit the tissue embedding box (3) and the control embedding box (4).

4. The dual-wax block synchronous slicing assembly according to claim 3, characterized in that: It also includes a trimming knife (6) for removing the wax block at the junction of the tissue sample wax block and the control sample wax block; the trimming knife (6) includes an inverted triangular blade (61) and a grip handle (62) fixedly connected to the inverted triangular blade (61).

5. The dual-wax block synchronous slicing assembly according to claim 4, characterized in that: It also includes a joint wax injection mold (63), which is removably and sealingly covered on the tissue embedding box (3) and the control embedding box (4) for filling the wax injection gap formed after excision with wax; The joint wax injection mold (63) includes: Flat plate (64), with a wax injection opening (65) in the middle; Two inclined plates (66) are formed on the two side walls of the wax injection opening (65) along the axial direction. Two stepped mounting plates (67) are formed at both ends of the flat plate (64) and are used to be mounted on the outer walls of the tissue embedding box (3) and the control embedding box (4). When the joint wax injection mold (63) is placed in place, the wax injection opening (65) is directly opposite the wax injection notch, and wax is injected into the wax injection notch through the wax injection opening (65) to fill and connect.

6. The dual-wax block synchronous slicing assembly according to claim 1, characterized in that: The reference plate (2) can be installed vertically and vertically in the mounting slot (11) through the mounting structure; The mounting structure includes: There are two mounting through holes (21), which are formed through the inner bottom wall of the mounting groove (11); There are two limit guide shafts (22), one end of which is fixedly installed at the bottom of the reference plate (2), and the other end extends outward after passing through the mounting through hole (21), and a threaded blind hole is provided on the other end; There are two elastic elements (24), which are respectively installed in the mounting groove (11) and respectively sleeved on the limiting guide shaft (22); Two mounting bolts (25) are screwed onto the threaded blind hole; Two limiting gaskets (26) are fitted onto the mounting bolt (25), and their outer diameter is larger than the inner diameter of the mounting through hole (21).

7. The dual-wax block synchronous slicing assembly according to claim 2, characterized in that, The clamping connector (5) includes: The main body (52) is adapted to be detachably mounted on the cryostat, and protrusions (53) are formed on both sides of the bottom, with a mounting position (54) formed between the two protrusions (53). The movable gripper (55) is movably mounted on the main body (52) via a movable connection structure; the movable gripper (55) includes an operation dial (56) and a clamping block (57) fixedly disposed at the bottom of the operation dial (56), the clamping block (57) being located within the mounting position (54); A clamping frame (58) is fixedly installed on the front of the main body (52) and located above the clamping block (57); an adjustable clamping opening (51) is formed between the clamping frame (58) and the clamping block (57).

8. The dual-wax block synchronous slicing assembly according to claim 7, characterized in that, The active connection structure includes: There are two internal thread blind holes (7), which are respectively opened on both sides of the main body (52). The inner wall of a section of the internal thread blind hole (7) is a smooth surface. There are two connecting studs (71), which are screwed into the internal thread blind hole (7) respectively. The inner part of the connecting stud (71) is a smooth cylindrical section. There are two guide holes (72), which are respectively opened on the main body (52) in the vertical direction. One end is connected to the internal thread blind hole (7), and the other end extends downward and passes through the clamping block (57). There are two shaft grooves (73), which are respectively opened at the bottom of the clamping block (57) and communicate with the guide hole (72); There are two spring shafts (74), which are respectively installed in the shaft groove (73); There are two tension springs (75), which are respectively inserted into the guide hole (72), with one end hanging on the smooth cylindrical section of the connecting stud (71) and the other end hanging on the spring shaft (74); Two threaded through holes (76) are respectively opened through the two protrusions (53); There are two blind holes (77) on the shaft, which are respectively opened on both sides of the clamping block (57); There are two rotating shaft bolts (78), which are screwed into the threaded through hole (76) respectively. The free end of the optical shaft extends into the rotating shaft blind hole (77) and is rotatably connected to the rotating shaft blind hole (77), thereby movably mounting the movable jaw (55) on the main body (52).

9. The dual-wax block synchronous slicing assembly according to claim 7, characterized in that: The main body (52) has a positioning groove (8) in the middle of the front side along the vertical direction. The width of the positioning groove (8) is greater than or equal to the width of the positioning plate (41), so that the positioning plate (41) can be installed in the positioning groove (8) to position the tissue embedding box (3) and the control embedding box (4). and / or; The operating dial (56) is movably installed in the positioning groove (8).

10. A method for simultaneous sectioning of two paraffin blocks, characterized in that, Includes the following steps: The clamping connector (5) is fixedly installed on the cryostat; Place the tissue embedding cassette (3) containing the tissue sample wax block between the limiting edges (13) on both sides of the tray (1) and press it down so that the tissue sample wax block is in close contact with the upper surface of the reference plate (2); place the control sample wax block on the reference plate (2) so that one end of it is aligned with the tissue sample wax block; snap the control embedding cassette (4) onto one side of the tissue embedding cassette (3) and cover the control sample wax block; inject wax into the control embedding cassette (4) so ​​that the control sample wax block is fixedly connected to the control embedding cassette (4); Take out the tissue embedding box (3) and the control embedding box (4) together as a whole, flip them over and place them between the limiting edges (13) on both sides of the tray (1), and install the positioning plate (41) in the positioning opening slot (14). Using a trimming knife (6), a notch is formed at the connection between the tissue sample wax block and the control sample wax block. The suture wax injection mold (63) is placed on the tissue embedding box (3) and the control embedding box (4), so that the wax injection opening (65) is directly opposite the notch left after the excision. Wax is injected into the notch through the wax injection opening (65) to fix the tissue sample wax block and the control sample wax block together. Move the operating lever (56) to drive the clamping block (57) to rotate downwards, expanding the space of the clamping opening (51). Place the tissue embedding box (3) and the control embedding box (4) that are snapped together into the clamping opening (51), so that the positioning plate (41) is installed in the positioning groove (8). Release the operating lever (56), and the clamping block (57) resets and cooperates with the clamping frame (58) to clamp and fix the tissue embedding box (3) and the control embedding box (4) in the clamping opening (51). A cryostat was used to simultaneously section paraffin blocks of tissue samples and control samples that were fixed together.

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