Electrochemical luminescence testing device

Abstract

The utility model relates to an electrochemical luminescence testing device, which comprises a supporting mechanism, a light-emitting mechanism, a light-emitting mechanism and a light-emitting mechanism, the supporting mechanism comprises a base and a support arranged on the base, the base is provided with a light-transmitting hole, and the support is annularly provided with a first step surface; the shading mechanism comprises a shading cover covering the support, a second step surface arranged on the shading cover, an air hole formed in the shading cover and a plurality of test holes formed in the shading cover, and the first step surface and the second step surface are matched to form a step shading contact surface; the testing hole is used for being connected with an electrode for electrochemical testing, and the light transmitting hole is used for being in threaded connection with analysis equipment for detection. Through the arrangement, the electrochemical luminescence detection cell is not influenced by external light noise, and the electrochemical luminescence detection cell can be adapted to different photomultipliers and optical equipment interfaces of the collimating mirror of the optical fiber spectrometer by changing the thread size of the light transmitting hole.

Description

Technical Field

[0001] The utility model relates to the field of testing devices, in particular to an electrochemical luminescence testing device. Background Art

[0002] Electrochemiluminescence (ECL), also known as electrochemiluminescence, is the product of the combination of chemiluminescence and electrochemistry. It refers to the generation of specific electrogenerated substances on the electrode surface by applying a certain voltage for electrochemical reaction, and then the excited state is formed between these electrogenerated substances or between certain components in the electrogenerated substance system through electron transfer, and the excited state returns to the ground state to produce a luminescent phenomenon. Electrochemiluminescence testing often requires the use of an electrochemiluminescence detection cell in conjunction with other analytical equipment to complete the test. At the same time, during the test, the equipment needs to be shielded from light to reduce the impact of external light noise on the test results.

[0003] Existing commercial electrochemiluminescence testing systems mainly adopt an integrated design, mainly including an electrochemiluminescence reaction cell, electrochemical testing and optical detection equipment. Users have limited choices when conducting electrochemiluminescence testing. The main reasons are: the bracket for fixing the electrochemiluminescence reaction cell is a non-standard structure, the shading structure of the electrochemiluminescence reaction cell is not compact, and the electrochemical workstation and optical detection instrument for the electrochemiluminescence test cannot be selected. If a split design is adopted, it is easily affected by external light noise. In addition, the detection equipment for electrochemiluminescence intensity signal or electrochemiluminescence spectral signal in the electrochemiluminescence testing system is not highly matched. In order to realize the detection of electrochemiluminescence intensity and spectral signals, users often need to purchase two electrochemiluminescence testing systems, which greatly increases the purchase and use costs of related equipment. Utility Model Content

[0004] To this end, the technical problem to be solved by the utility model is to overcome the problems of low adaptability of electrochemical luminescence testing devices in the prior art due to their integrated design and easy generation of light noise due to the split design, thereby providing an electrochemical luminescence testing device.

[0005] In order to solve the above technical problems, the utility model provides an electrochemical luminescence testing device, comprising:

[0006] The supporting mechanism comprises: a base, a bracket arranged on the base, the base is provided with a light-transmitting hole, and the bracket is provided with a first step surface;

[0007] The shading mechanism comprises: a shading cover arranged on the bracket, a second step surface opened on the shading cover, an air hole opened on the shading cover, and a plurality of test holes opened on the shading cover, wherein the first step surface and the second step surface are adapted to form a step light-shielding contact surface, the test hole is used to be connected to an electrode for electrochemical testing, and the light-transmitting hole is used to be threadedly connected to an analytical device for detection.

[0008] In one embodiment of the present invention, the light-transmitting hole and the plurality of test holes are arranged along the same direction, the plurality of test holes are arranged along the same plane, and at least one of the test holes and the light-transmitting hole are arranged coaxially.

[0009] In one embodiment of the present invention, at least the outer surfaces of the light shielding cover, the base and the bracket are provided with a light absorbing layer.

[0010] In one embodiment of the utility model, the light-transmitting hole is provided with a thread structure, and the thread structure is an internal thread or an external thread portion of the hole, and the external thread portion is provided on a side of the base close to the analysis device.

[0011] In one embodiment of the utility model, the light transmission hole is threadedly connected with a threaded member, the size of one end of the threaded member matches the thread size of the light transmission hole, and the size of the other end of the threaded member matches the thread size of the analysis device.

[0012] In one embodiment of the present invention, the test hole is provided with a third step surface, and the third step surface is provided on a side close to the electrode to be plugged.

[0013] In one embodiment of the utility model, the bracket and the base are integrally formed, the shape of the bracket is any one of square, rectangular, oblong or circular, the internal thread of the hole is composed of a plurality of coaxially arranged internal threaded holes, adjacent internal threaded holes have different sizes and are formed with step surfaces, and the inner diameters of the plurality of internal threaded holes gradually decrease along the assembly direction.

[0014] In one embodiment of the utility model, the light shielding cover, the base and the bracket enclose a light-proof space, the air holes and the test holes are arranged on the top surface of the light shielding cover, and the light-transmitting hole is arranged in the middle of the base.

[0015] In one embodiment of the utility model, a shading plate is arranged inside the shading cover, and the shading plate is horizontally arranged between the shading cover and the base. The shading cover is provided with a test inner hole and a breathable inner hole, and the test inner hole and the test hole are coaxially arranged, and the breathable inner hole and the breathable hole are staggered with different axes.

[0016] In one embodiment of the utility model, a detection electrode group is further included, and the detection electrode group includes: a reference electrode, a working electrode and a counter electrode, and the ends of the reference electrode, the working electrode and the counter electrode are provided with electrode sleeves, and the electrode sleeves are adapted to the third step surface.

[0017] The above technical solution of the utility model has the following advantages compared with the prior art:

[0018] The utility model discloses an electrochemical luminescence test device, wherein one side of the base is connected to the analysis device and the other side is used to support the detection cell, and a light-transmitting hole is provided at the bottom, which is used to transmit the optical signal generated during the test to the analysis device for detection. The bracket is fixed on the base, and is used to support and fix the light-shielding mechanism. The upper surface of the bracket is provided with a first step surface, which is used to form a light-shielding contact surface with the light-shielding mechanism, so as to further improve the light-shielding performance of the device, and a light-shielding cover is provided on the bracket, which is used to block external light and avoid interfering with the test process. The lower surface of the light-shielding cover is provided with a second step surface adapted to the first step surface, and the two step surfaces contact each other to form a step light-shielding contact surface, which effectively blocks external light from entering the test area, not only effectively forming a light-shielding space, ensuring that the electrochemical luminescence detection cell is not affected by external light noise, but also being able to adapt to different photomultiplier tubes and optical device interfaces of the optical fiber spectrometer collimator by changing the thread size of the light-transmitting hole, thereby improving the scope of application of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to make the content of the utility model easier to understand, the utility model is further described in detail according to the specific embodiments of the utility model in combination with the accompanying drawings, wherein

[0020] Figure 1 It is a three-dimensional diagram of the test device of the utility model;

[0021] Figure 2 It is a cross-sectional view of the test device of the utility model;

[0022] Figure 3 It is a cross-sectional view of the external thread structure of the utility model;

[0023] Figure 4 It is a three-dimensional diagram of the test device and the threaded member of the utility model;

[0024] Figure 5 It is a schematic diagram of the structure of the internal thread of the hole of the utility model;

[0025] Figure 6 It is a structural schematic diagram of the sunshade of the utility model.

[0026] Explanation of the reference numerals in the specification: 1. base; 2. bracket; 3. first step surface; 4. light shielding cover; 5. test hole; 6. ventilation hole; 7. second step surface; 8. light-transmitting hole; 9. external threaded portion; 10. threaded part; 11. internal thread of the hole; 12. test inner hole; 13. ventilation inner hole; 14. light shielding plate; 16. fixing hole. DETAILED DESCRIPTION

[0027] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0028] Example

[0029] Reference Figure 1-6 As shown, an electrochemical luminescence testing device of the utility model comprises:

[0030] The supporting mechanism comprises: a base 1, a bracket 2 arranged on the base 1, the base 1 is provided with a light-transmitting hole 8, and the bracket 2 is surrounded by a first step surface 3;

[0031] The shading mechanism comprises: a shading cover 4 covering the bracket 2, a second step surface 7 opened on the shading cover 4, an air hole 6 opened on the shading cover 4, and a plurality of test holes 5 opened on the shading cover 4, wherein the first step surface 3 and the second step surface 7 are adapted to form a step light-shielding contact surface, the test hole 5 is used to be connected to an electrode for electrochemical testing, and the light-transmitting hole 8 is used to be threadedly connected to an analytical device for detection.

[0032] The utility model discloses an electrochemical luminescence test device, wherein the base 1 is fixed by a fixing hole 16, and the specific fixing method can be fixed by a detachable method such as bolts or pins. One side of the base 1 is connected to the analysis device and the other side is used to support the detection pool, and a light-transmitting hole 8 is provided at the bottom 1, which is used to transmit the optical signal generated during the test to the analysis device for detection. The bracket 2 is fixed on the base 1, and is used to support and fix the shading mechanism. The upper surface of the bracket 2 is provided with a first step surface 3, which is used to form a light-shielding contact surface with the shading mechanism to further improve the light-shielding performance of the device. The light-shielding cover 4 is provided on the bracket 2 to block external light and avoid interference with the test process. The lower surface of the light-shielding cover 4 is provided with a second step surface 7 that is compatible with the first step surface 3. The two step surfaces contact each other to form a step light-shielding contact surface, which effectively blocks external light from entering the test area. The light-shielding cover 4 is also provided with air holes 6 for ensuring air circulation in the test area, or connected to a bubble tube to maintain a suitable test environment. The multiple test holes 5 on the light-shielding cover 4 are used to connect electrodes for electrochemical testing, and the electrodes are inserted into the test holes 5 to close the test holes 5.

[0033] When the electrochemical luminescence test device is used for testing, the base is first fixed through the fixing hole, the electrochemical cell is placed in the bracket 2 and fixed, the light-shielding cover is set on the bracket and the electrode is inserted through the test hole, so that the first step surface 3 and the second step surface 7 are in close contact, forming a light-shielding contact surface to block external light. During the test, a certain voltage is applied to perform an electrochemical reaction, and specific electrogenerated substances are generated on the electrode surface. Then, the excited state is formed by electron transfer between the electrogenerated substances or between the components in the electrogenerated substances system, and the excited state returns to the ground state to emit light, and the light-transmitting hole 8 transmits the luminescent signal to the detection and analysis equipment under the base 1 to realize the detection and analysis of the luminescent signal.

[0034] See also Figure 2 , Figure 3 As shown, the light-transmitting hole 8 and the plurality of test holes 5 are arranged along the same direction, the plurality of test holes 5 are arranged along the same plane, at least one of the test holes 5 and the light-transmitting hole 8 are arranged coaxially, and the light-transmitting hole 8 and the test hole 5 are in a direction perpendicular to the base 1 and the light-shielding cover 4, ensuring that the light signal generated during the test process can be efficiently transmitted to the detection analysis equipment through the light-transmitting hole 8, which not only facilitates the connection of the electrochemical test electrode with the sample to be tested through the test hole 5, but also facilitates the optical equipment to collect the luminescent signal at the electrochemical luminescent working electrode through the light-transmitting hole 8

[0035] Continue to see Figure 2 , Figure 3 As shown, multiple test holes 5 are arranged along the same plane, that is, all test holes 5 are located in the same horizontal plane, so that the contact positions of all electrochemical test electrodes and the samples to be tested are at the same height, so that the electrical signal transmission during the test is more uniform and stable, and the consistency of the test results is improved. In this embodiment, at least one test hole 5 and the light-transmitting hole 8 are coaxially arranged, that is, the central axis of at least one test hole 5 coincides with the central axis of the light-transmitting hole 8. When the test hole 5 and the light-transmitting hole 8 are coaxially arranged, the signal-to-noise ratio and reliability of the test results are improved.

[0036] At least the outer surface of the light shielding cover 4, the base 1 and the bracket 2 is provided with a light absorbing layer. The light shielding cover 4, the base 1 and the bracket 2 are made of a light absorbing material as a whole or are provided with a light absorbing layer on the outer surface. The black material is any one of an aluminum alloy, a stainless steel material or a 3D printed polymer material whose surface is anodized to black. The light absorbing layer can be made of a light absorbing paint, a light absorbing film or a light absorbing material. The material of the light absorbing layer is selected from materials with good light absorbing properties such as black matte paint, carbon black, and black oxide. The light absorbing layer can be provided on the outer surface of the light shielding cover 4, the base 1 and the bracket 2 by spraying, coating, attaching, etc. The light absorbing layer can effectively absorb the incident light, reduce the reflection and scattering of the light, further improve the light-shielding performance of the device, provide a more ideal dark environment for the test process, further reduce external light noise, reduce the interference of stray light on the test process, and improve the signal-to-noise ratio and sensitivity of the test results.

[0037] The light-transmitting hole 8 is provided with a threaded structure, and the size and corresponding thread of the light-transmitting hole 8 can be changed according to the interface of the connected photomultiplier tube and the optical device of the optical fiber spectrometer collimator. The threaded structure is an internal thread 11 or an external threaded portion 9, and the external threaded portion 9 is arranged on the side of the base 1 close to the analysis device, and the external thread is arranged on the outer wall of the base 1 facing the analysis device. The diameter of the light-transmitting hole 8 is designed according to the optical path system of the analysis device and the analysis requirements, and the thread is a triangular thread, a trapezoidal thread, a rectangular thread or a serrated thread, etc.

[0038] Furthermore, the external threaded portion 9 is arranged on the outer wall of the light transmission hole 8 on the side close to the analysis device, which is convenient for connecting the light transmission hole 8 with the optical path system of the analysis device. By cooperating with the nut structure inside the analysis device, the light transmission hole 8 and the analysis device can be quickly connected and disassembled, and it also has the advantages of accurate positioning and reliable connection.

[0039] See also Figure 4As shown, the light-transmitting hole 8 is threadedly connected with a threaded member 10, the size of one end of the threaded member 10 is adapted to the thread size of the light-transmitting hole 8, and the size of the other end of the threaded member 10 is matched with the thread size of the analysis device. In order to realize the connection between the light-transmitting hole 8 and the analysis device, the light-transmitting hole 8 is threadedly connected with the threaded member 10, the size of one end of the threaded member 10 is adapted to the thread structure, and the size of the other end is matched with the thread size of the analysis device. In this way, the light-transmitting hole 8 and the analysis device can be conveniently and reliably fixed by threading the two ends of the threaded member 10 with the light-transmitting hole 8 and the analysis device, and the other end of the threaded member 10 is also provided with a thread matching the analysis device, and the thread of the other end of the threaded member 10 is an internal thread or an external thread. By setting it as an external thread, the test device can be installed on the analysis device. During installation, one end of the threaded member 10 is screwed into the internal thread of the light-transmitting hole 8 or inserted into the external thread and tightened, and then the other end of the threaded member 10 is connected to the analysis device. By replacing different threaded members 10, different electrochemical cells or optical detection devices can be adapted.

[0040] In order to avoid interference of external light on the test, the test hole 5 is provided with a third step surface, which is located on the side of the test hole 5 close to the electrode to be plugged in. By setting the third step surface, another shading structure can be formed, so that after the plug-in electrode is inserted into the test hole 5, the space between the plug-in electrode and the test hole 5 can remain in a light-proof state, thereby reducing interference from external light.

[0041] The bracket 2 and the base 1 are integrally formed, and the shape of the bracket 2 is any one of square, rectangular, oblong or circular (matching different electrochemical cells), and is manufactured by injection molding, die casting, 3D printing, etc., so that the bracket 2 and the base 1 form a whole, thereby improving the integration and stability of the device and simplifying the assembly process of the device. The shape of the bracket 2 can be selected from any one of square, rectangle, oblong or circular according to actual needs to meet different test requirements and spatial layouts. The inner thread 11 of the hole is composed of a plurality of coaxially arranged inner threaded holes. The adjacent inner threaded holes have different sizes and are formed with step surfaces. The inner diameters of the plurality of inner threaded holes gradually decrease along the assembly direction, and the docking analysis equipment can be conveniently installed by threaded connection. The adjacent inner threaded holes have different sizes and are formed with step surfaces. The inner diameters of the plurality of inner threaded holes gradually decrease along the assembly direction. The assembly direction is the assembly direction of the analysis equipment and the base 1. In this embodiment, the assembly direction is from the bottom to the top of the base 1, and it can adapt to a plurality of analysis equipment with corresponding external thread sizes. Specifically, the larger external thread is connected to the larger external thread on the outside, and the smaller external thread penetrates into the inner thread 11 of the hole and is connected to the smaller internal thread on the inside.

[0042] The light shielding cover 4, the base 1 and the bracket 2 enclose a light-proof space, the air holes 6 and the test holes 5 are arranged on the top surface of the light shielding cover 4, the air holes 6 are arranged in the middle of the base 1, and the light shielding cover 4 is arranged on the top of the device to block external light from entering the light-proof space. The top surface of the light shielding cover 4 is provided with air holes 6 and the test holes 5. The air holes 6 are used for gas exchange between the light-proof space and the outside world, and are connected to an external nitrogen pipe through the air holes to pass nitrogen protection to the space in the detection pool, and the inert gas protection is used to prevent the components in the air from having irrelevant reactions in the detection pool and affecting the detection.

[0043] See also Figure 6 As shown, a shading plate 14 is arranged in the shading cover 4, and the shading plate 14 is horizontally arranged between the shading and the base 1. The shading cover 4 is provided with a test inner hole 12 and a breathable inner hole 13. The test inner hole 12 and the test hole 5 are coaxially arranged, and the breathable inner hole 13 and the breathable hole 6 are staggered in different axes. The purpose of light protection of the double-layer plate is achieved by staggering the breathable hole 6 and the breathable inner hole 13. The light passing through the gap of the outer breathable hole 6 cannot enter the detection pool through the breathable inner hole 13, and is reflected and absorbed between the shading cover 4 and the shading plate 14.

[0044] It also includes a detection electrode group, which includes: a reference electrode, a working electrode and a counter electrode. The ends of the reference electrode, the working electrode and the counter electrode are provided with electrode sleeves, and the electrode sleeves are matched with the third step surface.

[0045] Obviously, the above embodiments are merely examples for the purpose of clear explanation and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from these are still within the scope of protection of the invention of the present utility model.

Claims

1. An electrochemiluminescence testing device, characterized in that: include: A support mechanism, comprising: a base, a bracket disposed on the base, the base having a light-transmitting hole, the bracket ring having a first step surface; The shading mechanism comprises: a shading cover arranged on the bracket, a second step surface opened on the shading cover, an air hole opened on the shading cover, and a plurality of test holes opened on the shading cover, wherein the first step surface and the second step surface are adapted to form a step light-shielding contact surface, the test hole is used to be connected to an electrode for electrochemical testing, and the light-transmitting hole is used to be threadedly connected to an analytical device for detection.

2. An electrochemiluminescence testing device according to claim 1, characterized in that: The light-transmitting hole and the plurality of test holes are arranged along the same direction, the plurality of test holes are arranged along the same plane, and at least one of the test holes and the light-transmitting hole are arranged coaxially.

3. An electrochemiluminescence testing device according to claim 2, characterized in that: At least the outer surfaces of the light-shielding cover, the base and the bracket are provided with a light-absorbing layer.

4. An electrochemiluminescence testing device according to claim 1, characterized in that: The light-transmitting hole is provided with a thread structure, and the thread structure is an internal thread or an external thread portion of the hole, and the external thread portion is provided on a side of the base close to the analysis device.

5. An electrochemiluminescence testing device according to claim 4, characterized in that: The light-transmitting hole is threadedly connected with a threaded member, the size of one end of the threaded member matches the thread size of the light-transmitting hole, and the size of the other end of the threaded member matches the thread size of the analysis device.

6. An electrochemiluminescence testing device according to claim 1, characterized in that: The test hole is provided with a third step surface, and the third step surface is arranged on a side close to the electrode to be plugged.

7. An electrochemiluminescence testing device according to claim 4, characterized in that: The bracket and the base are integrally formed, and the shape of the bracket is any one of square, rectangular, oblong or circular. The internal thread of the hole is composed of a plurality of coaxially arranged internal threaded holes, and the adjacent internal threaded holes have different sizes and are formed with step surfaces. The inner diameters of the plurality of internal threaded holes gradually decrease along the assembly direction.

8. An electrochemiluminescence testing device according to claim 1, characterized in that: The light-shielding cover, the base and the bracket enclose a light-shielding space, the air holes and the test holes are arranged on the top surface of the light-shielding cover, and the air holes are arranged in the middle of the base.

9. An electrochemiluminescence testing device according to claim 1, characterized in that: A shading plate is arranged inside the shading cover, and the shading plate is arranged horizontally between the shading plate and the base. The shading cover is provided with a test inner hole and a breathable inner hole, and the test inner hole and the test hole are arranged coaxially, and the breathable inner hole and the breathable hole are arranged in a staggered manner with different axes.

10. An electrochemiluminescence testing device according to any one of claims 1 to 9, characterized in that: It also includes a detection electrode group, which includes: a reference electrode, a working electrode and a counter electrode. The ends of the reference electrode, the working electrode and the counter electrode are provided with electrode sleeves, and the electrode sleeves are matched with the third step surface.

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