Membrane washing, incubating and refrigerating all-in-one machine

Abstract

The utility model discloses a membrane washing, incubating and refrigerating all-in-one machine which comprises a shell, an incubation box and a refrigeration box, wherein a containing cavity is formed in the shell; the panel is arranged at the front end of the shell in an openable and closable manner; the kit assembly is arranged at the bottom in the accommodating cavity; the incubation box assembly is arranged on the right side of the kit assembly, and a plurality of antibody incubation grooves are distributed in the incubation box assembly; the swinging table is arranged at the bottom of the accommodating cavity and is used for driving the incubation box assembly to obliquely rotate and swing front and back; a plurality of gun heads are inserted into the gun head frame, and the gun head frame is arranged on one side of the swing table and can move front and back along the swing table; the waste liquid box is arranged on one side of the incubation box assembly or the kit assembly, and the cleaning liquid box is arranged on one side of the waste liquid box; the pipetting assembly comprises a pipettor used for grabbing any gun head on the gun head frame and a driving device used for driving the pipettor to move in the longitudinal direction and the horizontal direction. According to the utility model, the functions of automatic sample adding, automatic recovery, automatic cleaning, automatic incubation and the like are realized, and the automation degree is high.

Description

Technical Field

[0001] The utility model belongs to the technical field of incubation instruments, and in particular relates to a membrane washing and incubation integrated machine with refrigeration. Background Art

[0002] Western, also known as Western blotting, Western blotting, and Western blotting, is one of the important methods for detecting proteins with antibodies. In molecular biology, biochemistry, and immunogenetics research, protein blotting (immunoblotting test) is a commonly used experimental method. Its basic principle is to stain cells or biological tissue samples treated with gel electrophoresis with specific antibodies. By analyzing the position and depth of staining, information on the expression of specific proteins in the analyzed cells or tissues is obtained. The Western blotting method uses polyacrylamide gel electrophoresis, the object to be detected is protein, the "probe" is antibody, and the "color development" uses labeled secondary antibodies. The protein sample separated by PAGE (polyacrylamide gel electrophoresis) is transferred to a solid phase carrier (such as nitrocellulose membrane), which adsorbs the protein in the form of non-covalent bonds and can keep the type of polypeptide separated by electrophoresis and its biological activity unchanged. The protein or polypeptide on the solid phase carrier is used as an antigen to react with the corresponding antibody, and then react with the enzyme or isotope-labeled second antibody. The protein components expressed by the specific target gene separated by electrophoresis are detected through substrate color development or radioautography. This technology is also widely used to detect the expression of protein levels.

[0003] The experimental steps of Western Blot include electrophoresis, transfer, blocking, first membrane wash, incubation with primary antibody, second membrane wash, incubation with secondary antibody, third membrane wash, and color development. The conventional practice is to wash the membrane three times, each wash lasting 10 minutes. Depending on the experimental needs, the washing time may sometimes be longer. The first and second membrane washes do not require protection from light, and the third membrane wash may or may not be protected from light depending on the color development method.

[0004] Currently, Western Blot experiments are usually performed manually, requiring manual addition of sample liquid. After the experiment, the experimental reagents must be manually poured out and manually cleaned. The entire process is very cumbersome and inconvenient, with a low degree of automation and high workload for experimenters. If any operation goes wrong, the operation must be restarted, which is very time-consuming. In addition, due to incomplete manual operation, residual liquid or uneven sample addition may occur. For some products with non-replaceable gun tips, there is a possibility of cross-contamination during the process of aspirating multiple reagents, resulting in inaccurate experimental data. Utility Model Content

[0005] The utility model aims to overcome the existing technical defects and provide a membrane washing and incubation integrated machine with refrigeration, which can automatically realize the processes of automatic sample addition, automatic incubation, automatic recovery, automatic cleaning, etc. after protein transfer and before substrate incubation, with a high degree of automation, ensuring the stability and accuracy of test data.

[0006] In order to solve the above technical problems, the utility model provides a membrane washing and incubation integrated machine with refrigeration, comprising:

[0007] A shell having a cavity inside and an opening at the front side of the shell;

[0008] A panel, which is closable and arranged at the front end of the housing and is used to cover or open the opening;

[0009] A reagent kit assembly, which is arranged at the bottom of the chamber and is used to separate and accommodate a plurality of different reagents;

[0010] An incubation box assembly, which is arranged on the right side of the test kit assembly, and a plurality of antibody incubation tanks are arranged on the incubation box assembly;

[0011] A swing platform, which is arranged at the bottom of the cavity and is used to drive the incubation box assembly to tilt, rotate and swing forward and backward;

[0012] A gun head rack, on which a plurality of gun heads are inserted, and the gun heads are mounted on one side of the swing platform and can move forward and backward along the swing platform;

[0013] A waste liquid box, which is arranged on the right side of the incubation box assembly or the left side of the reagent kit assembly and is used to collect waste liquid;

[0014] A cleaning liquid box, which is arranged on the right side or the left side of the waste liquid box and is used to contain the cleaning liquid;

[0015] The pipetting assembly includes a pipette arranged at the upper end of the cavity and used to grab any gun tip on the gun tip rack, and a driving device used to drive the pipette to move in the longitudinal and horizontal directions, so that the gun tip can move back and forth between the gun tip rack, the reagent kit assembly, the incubation box assembly, the waste liquid box and the cleaning liquid box.

[0016] Furthermore, the membrane washing and incubation belt refrigeration integrated machine also includes a vertical moving device for driving the panel to slide up and down and open, and the vertical moving device includes two transmission belts which are respectively arranged on both sides of the front end of the shell and vertically arranged, a transmission shaft connected to the two transmission belts, and a first motor for driving the transmission shaft to be connected to the transmission shaft. The front end of the shell is also provided with two guide shafts which are respectively located in front of the two transmission belts, and a slider is slidably provided on the guide shaft, one side of the slider is fixedly connected to the transmission belt, and the inner upper end or lower end of the panel is fixedly connected to the slider on the two guide shafts; first synchronous pulleys are provided at both ends of the transmission shaft, and two second synchronous pulleys which are respectively located below the first synchronous pulley and are rotatably provided in the shell, and the transmission belt is wound around the first synchronous pulley and the second synchronous pulley; a driven gear is also provided on the transmission shaft, and a driving gear meshing with the driven gear is provided on the output shaft of the first motor.

[0017] Furthermore, the reagent kit assembly includes a reagent kit base, a reagent kit upper cover rotatably arranged on the upper end of the reagent kit base, and at least three reagent kits arranged left and right between the reagent kit base and the reagent kit upper cover and used to respectively accommodate different reagents, and at least one first through hole for the gun tip to pass through is provided in the reagent kit upper cover at a position corresponding to each reagent kit, a first cooling box and a first temperature conduction plate provided on the upper end of the first cooling box are provided inside the reagent kit base, the reagent kit is provided on the first temperature conduction plate, a water inlet and a water outlet are provided on the first cooling box, thermal insulation cotton is provided between the first cooling box and the reagent kit base, and a temperature sensor is provided on the first temperature conduction plate; handles are provided on the reagent kit upper cover and the front end of the reagent kit.

[0018] Furthermore, the incubation box assembly includes an incubation box base, an incubation box upper cover which is rotatably arranged on the upper end of the incubation box base, and at least three incubation boxes arranged left and right between the incubation box base and the incubation box upper cover, the upper end of the incubation box is recessed with at least one antibody incubation groove arranged left and right, and the incubation box upper cover is provided with at least three second through holes arranged left and right at the position corresponding to each incubation box for the gun tip to pass through; a second cooling box and a second heat conduction plate arranged on the upper end of the second cooling box are provided inside the incubation box base, the incubation box is provided on the second heat conduction plate, a water inlet and a water outlet are provided on the second cooling box, heat insulation cotton is provided between the second cooling box and the incubation box base, and a temperature sensor is provided on the second heat conduction plate; a handle is provided at the front end of the incubation box upper cover.

[0019] Furthermore, the membrane washing and incubation belt refrigeration integrated machine also includes a camera arranged above the incubation box assembly and used for irradiating downwards.

[0020] Furthermore, the rocking platform includes a base arranged at the bottom of the cavity, a second motor arranged on the base, and a crank-connecting rod assembly that transmission connects the second motor and the incubation box base, the crank-connecting rod assembly including a crank at one end fixed to the output shaft of the second motor and a connecting rod at one end rotatably connected to the other end of the crank, the middle of the bottom rear side of the incubation box base is rotatably connected to the other end of the connecting rod through a connecting plate; the upper end of the base is also symmetrically provided with two support seats for supporting the front end of the incubation box assembly, and the bottom front side of the incubation box base is provided with two connecting members that are rotatably connected to the support seats in a one-to-one correspondence; the test kit assembly is arranged on the base and is located on one side of the incubation box assembly, and one end of the base is provided with a bottom box located on the other side of the incubation box assembly, and the bottom box is concavely provided with two grooves for respectively accommodating the cleaning liquid box and the waste liquid box, and the front ends of the grooves are openings; the front ends of the cleaning liquid box and the waste liquid box are both provided with handles.

[0021] Furthermore, a Y-axis driving device for driving the gun head rack to move forward and backward is provided in the base, a sliding groove extending forward and backward along the base is opened on one side of the base, and the Y-axis driving device includes a sliding seat provided in the base and extending forward and backward along the base, a slider slidably provided on the sliding seat, a Y-axis screw rod rotatably provided on the sliding seat and extending forward and backward along the base, and a third motor driving the Y-axis screw rod to rotate, the slider is threadedly connected to the Y-axis screw rod, the slider is provided with a supporting plate with one end extending out of the sliding groove, and the gun head rack is arranged on the supporting plate outside the base.

[0022] Furthermore, the membrane washing and incubation belt refrigeration integrated machine also includes a water tank arranged in the outer shell, a third cooling box connected to the water tank for circulation through a pipeline, and a refrigerator abutting the surface of the third cooling box, one end of the third cooling box is provided with a water inlet and a water outlet connected to the water tank respectively, and an internal circulation pump is provided in the pipeline connecting the water inlet and the water tank; the water inlet and water outlet of the first cooling box and the second cooling box are connected to the water tank through pipelines respectively, and a first water pump is provided on the pipeline connecting the water tank and the water inlet of the first cooling box, and a second water pump is provided on the pipeline connecting the water tank and the water inlet of the second cooling box; a liquid level sensor for detecting the liquid level and a temperature sensor for detecting the temperature of the liquid in the water tank are provided in the water tank.

[0023] Furthermore, the driving device includes a vertical driving device for driving the pipette to move longitudinally and a horizontal driving device for driving the pipette and the vertical driving device to move horizontally; the vertical driving device is provided with a first travel switch for limiting the up and down travel of the clamping member, and the left and right ends of the horizontal driving device are provided with a second travel switch for limiting the left and right travel of the vertical driving device.

[0024] Furthermore, a touch screen for human-computer interaction and display is provided on one side of the shell. A rotating head that rotates longitudinally is rotatably provided on one side of the shell, and a rotating seat that rotates horizontally is rotatably provided at the lower end of the rotating head. The rear side of the touch screen is fixed on the rotating seat.

[0025] The utility model has the following beneficial effects:

[0026] 1. A pipette that can move up and down and left and right between a waste liquid box, a reagent kit component and an incubation box component is used, and the pipette cooperates with a gun head to realize the purpose of automatic liquid aspiration and automatic liquid discharge, so that the various reagents (such as blocking solution, primary antibody and secondary antibody) in the reagent kit component can be aspirated and discharged into the antibody incubation tank in the incubation box component by using the gun head for swing incubation, and the gun head can be replaced, and different gun heads are used to aspirate different reagents to avoid cross contamination between the reagents; after incubation, the primary antibody and secondary antibody to be recovered are aspirated and discharged back to the corresponding positions in the reagent kit component; the cleaning liquid is then aspirated by the gun head and transferred to the antibody incubation tank to clean the used antibody incubation tank; the liquid after cleaning in the antibody incubation tank is aspirated and discharged into the waste liquid box by using the gun head, and the aspiration of the cleaning liquid and the discharge of the waste liquid are not the same gun head as the reagents, so as to avoid the contamination of the gun head, thereby realizing the functions of automatic sample addition, automatic recovery, automatic cleaning, automatic incubation, etc., with a high degree of automation, ensuring the stability and accuracy of the test data.

[0027] 2. The reagents used in the experiment, such as blocking solution, primary antibody, and secondary antibody, are all proteins, which will slowly denature at room temperature. The entire experimental process takes several hours. If the protein reagents are not temperature-controlled, protein denaturation may affect the experimental results. At the same time, expensive reagents such as primary antibodies and secondary antibodies cannot be reused. Therefore, in the utility model, a cooling box is added to the bottom of the test kit and the incubation box, and the cooling box is used to cool the two, and the temperature of the protein reagents in the two is controlled by the use of a temperature sensor.

[0028] 3. The internal water tank is connected to three pump bodies, and the three pump bodies are used to realize independent control of three water circuits. One is used for self-circulating refrigeration of the liquid in the water tank, the second is used to connect with the reagent box to cool the reagent box, and the third is used to connect with the incubation box to cool the incubation box. The three circuits do not interfere with each other. One or two circuits can be opened for use separately, or all three circuits can be used together.

[0029] Additional aspects and advantages of the present invention will be partially given in the following description, which will become apparent from the following description or be understood through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The drawings described herein are used to provide a further understanding of the present invention, constitute a part of the present application, and do not constitute an improper limitation of the present invention. In the drawings:

[0031] Figure 1 This is a schematic diagram of a membrane washing and incubation machine with refrigeration in the embodiment;

[0032] Figure 2 This is a schematic diagram of the membrane washing, incubation and refrigeration integrated machine in the embodiment after the panel is removed;

[0033] Figure 3 This is a schematic diagram of the membrane washing, incubation and refrigeration integrated machine in the embodiment after removing the panel and the shell

[0034] Figure 4 It is a schematic diagram of the embodiment in which the panel and the vertical moving device are assembled and in an unopened state;

[0035] Figure 5 This is a schematic diagram of the interior of the gun tip rack, the reagent kit assembly, the incubation box assembly, the rocking platform, the waste liquid box, and the cleaning liquid box after assembly in the embodiment;

[0036] Figure 6 for Figure 5 Schematic diagram of another perspective;

[0037] Figure 7 for Figure 5 Schematic diagram of the midsole side;

[0038] Figure 8 for Figure 5 Front view of the

[0039] Fig. 9 for Figure 5 A cross-sectional view of the reagent box;

[0040] Fig.10 for Figure 5 Schematic diagram after removing the upper cover of the reagent kit and the upper cover of the incubation box;

[0041] Fig.11 Schematic diagram of the kit components in the embodiment;

[0042] Fig.12 is a cross-sectional view of a kit component in the embodiment;

[0043] Fig.13 A schematic diagram of an incubation box assembly in an embodiment;

[0044] Fig.14 is a cross-sectional view of an incubation box assembly in an embodiment;

[0045] Fig.15 is a cross-sectional view of a waste liquid box or a cleaning liquid box in an embodiment;

[0046] Fig.16 This is a schematic diagram of the assembly of the gun tip and the pipetting assembly in the embodiment;

[0047] Fig.17 Schematic diagram of the pipe connection between the water tank, water pump and cooling box in the embodiment

[0048] Fig.18 It is a schematic diagram of the touch screen after being fixed to the rotating head and the rotating base in the embodiment. DETAILED DESCRIPTION

[0049] In order to more fully understand the technical content of the present invention, the present invention will be further introduced and explained in conjunction with the accompanying drawings and specific embodiments below; it should be noted that descriptions such as "first" and "second" in the text are used to distinguish different components, etc., and do not represent the order of precedence, nor do they limit the "first" and "second" to different types.

[0050] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments; based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the utility model.

[0051] Example

[0052] like Figures 1 to 18As shown, the membrane washing and incubation integrated machine with refrigeration shown in this embodiment includes a shell 1, a panel 2, and a reagent kit component 3, an incubation box component 4, a swing platform, a pipetting component, a waste liquid box 5, a cleaning liquid box 6 and a gun head rack 7 in the shell 1. A cavity is provided inside the shell 1 for accommodating the above components. An opening is provided on the front side of the shell 1 to expose the internal components. The panel 2 can be opened and closed and is arranged at the front end of the shell 1 and is used to cover or open the opening; the reagent kit component 3 is arranged at the bottom of the cavity and is used to separate and accommodate a variety of different reagents. The conventionally accommodated reagents include blocking liquid, primary antibody and secondary antibody; the incubation box component 4 is arranged on the right side of the reagent kit component 3, and a plurality of antibody incubation grooves 44 are arranged on the incubation box component 4 for antibody incubation; the swing platform is arranged at the bottom of the cavity and is used to drive the incubation box component to tilt, rotate and swing back and forth, that is, the incubation box component 4 can be rotated within a certain angle range to swing the reagents entering the antibody incubation groove 44; the waste liquid box 5 It is arranged on the left side of the reagent kit component 7 and is used to collect waste liquid; a plurality of gun tips 71 are inserted on the gun tip rack 7, and the gun tip rack 7 is arranged on one side of the swing table and can move forward and backward along the swing table to realize the forward and backward movement and position change of each gun tip, so that the upper pipette can grab any gun tip on the gun tip rack conveniently; the waste liquid box 5 is arranged on the right side of the incubation box component 4 or the left side of the reagent kit component 3 and is used to collect waste liquid; the cleaning liquid box 6 is arranged on the right side or left side of the waste liquid box 5 and is used to accommodate the cleaning liquid; the pipette component It includes a pipette 72 arranged at the upper end of the cavity and used to grab any gun tip 71 on the gun tip rack 7, and a driving device for driving the pipette 72 to move in the longitudinal and horizontal directions, so that the pipette 72 can move back and forth between the gun tip rack 7, the reagent kit assembly 3, the incubation box assembly 4, the waste liquid box 5 and the cleaning liquid box 6. After grabbing the gun tip, the pipette can transfer the reagent in the reagent kit assembly 3 and the cleaning liquid in the cleaning liquid box 6 to the incubation box assembly, and can transfer the waste liquid to the waste liquid box.

[0053] In the above, a pipette that can move up and down and left and right between the waste liquid box, the reagent kit assembly and the incubation box assembly is used, and it cooperates with the gun head to achieve the purpose of automatic liquid suction and automatic liquid discharge, so that the gun head can be used to absorb and discharge the reagents in the reagent kit assembly (such as blocking solution, primary antibody and secondary antibody) into the antibody incubation tank in the incubation box assembly for swing incubation, and the gun head is replaceable, and different gun heads are used to absorb different reagents to avoid cross contamination between the reagents; after incubation, the primary antibody and secondary antibody to be recovered are absorbed and discharged back to the corresponding positions in the reagent kit assembly; then the cleaning liquid is absorbed by the gun head and transferred to the antibody incubation tank to clean the used antibody incubation tank; the liquid after cleaning in the antibody incubation tank is absorbed and discharged into the waste liquid box by the gun head, and the absorption of the cleaning liquid and the discharge of the waste liquid are not the same gun head as the reagents, so as to avoid the contamination of the gun head, thereby realizing the functions of automatic loading, automatic recovery, automatic cleaning, automatic incubation, etc., with a high degree of automation, ensuring the stability and accuracy of the test data.

[0054] In one embodiment, if Figure 3 to Figure 4 As shown, the membrane washing and incubation belt refrigeration integrated machine also includes a vertical moving device for driving the panel 2 to slide up and down and open, the vertical moving device includes two transmission belts 20 respectively arranged on both sides of the front end of the shell 1 and arranged vertically, a transmission shaft 21 rotatably arranged inside the shell 1 through a bearing and connected to the two transmission belts 20, and a first motor 22 for driving the transmission shaft 21 to be connected to the transmission. The front end of the shell 1 is also provided with two guide shafts 23 respectively located in front of the two transmission belts 20, and a slider 24 is slidably provided on the guide shaft 23, one side of the slider 24 is fixedly connected to the transmission belt 20, and the inner upper end or lower end of the panel 2 is connected to the two guide shafts 23. The slider 24 on the shaft 23 is fixedly connected; a first synchronous pulley 25 is provided at both ends of the transmission shaft 21, and two second synchronous pulleys 26 are rotatably provided in the housing 1 and are respectively located below the first synchronous pulley 25, and the transmission belt 20 is wound around the first synchronous pulley 25 and the second synchronous pulley 26; a driven gear 27 is also provided on the transmission shaft 21, and a driving gear 28 meshing with the driven gear 27 is provided on the output shaft of the first motor 22; in this structure, the first motor 22 is used to drive the transmission shaft 21 to rotate, thereby driving the transmission belt 20 to rotate up and down and the slider to slide up and down, thereby driving the panel 2 to be lifted up and down to open or close.

[0055] Preferably, a tensioning wheel 29 is rotatably provided in the housing and is located on one side of the transmission belt 20 to tension the transmission belt.

[0056] In one embodiment, in order to prevent the lifting door from causing dangerous behaviors such as pinching hands during the up and down movement, the first motor 22 is preferably a double-headed reduction DC motor, one end of which is transmission-connected to the transmission shaft 21, and the other end is transmission-connected to the encoder 221. When the panel descends and closes, it can stop immediately if there is an object blocking it from below, to prevent hands or objects from being pinched.

[0057] In one embodiment, if Figures 5 to 12As shown, the reagent kit assembly 3 includes a reagent kit base 30, a reagent kit upper cover 31 rotatably mounted on the upper end of the reagent kit base 30, and three reagent kits 32 arranged between the reagent kit base 30 and the reagent kit upper cover 31 and used to respectively accommodate different reagents. The three reagent kits 32 are respectively used to accommodate a blocking solution, a primary antibody and a secondary antibody. At least one first through hole 33 for a gun tip 71 to pass through is provided in the reagent kit upper cover 31 at a position corresponding to each reagent kit 32, which is used to absorb the internal liquid or discharge the liquid after the gun tip 71 passes through. When the reagent kit is provided with two accommodating grooves, there are two corresponding first through holes above them; a first cooling box 34 and a first temperature conducting plate 35 arranged at the upper end of the first cooling box 34 are provided inside the reagent kit base 30. The first cooling box 34 is used to cool the reagent kit 32. The reagent kit 32 is provided at The first temperature conduction plate 35 can better conduct the temperature to the liquids in the three reagent kits 32. The first cooling box 34 is provided with a water inlet 13 and a water outlet 14 for connecting with an external water supply device to form a circulating water cooling structure. A thermal insulation cotton 36 is provided between the first cooling box 34 and the reagent kit base 30 for insulation to reduce excessive loss of useless temperature. At the same time, after the reagent kit cover 31 is closed, the loss of internal temperature can be reduced to better maintain the temperature balance in the reagent kit. A temperature sensor 37 for detecting its temperature is provided on the first temperature conduction plate 35 to monitor the temperature change in real time to ensure the temperature control of the protein reagent inside the reagent kit, and the temperature can be as low as about 4°. The reagent kit cover 31 and the front end of the reagent kit 32 are both provided with handles 38 to facilitate flipping open the reagent kit cover 31 and taking out or putting in the reagent kit 32.

[0058] In one embodiment, if Figure 14 to Figure 15As shown, the incubation box assembly 4 includes an incubation box base 40, an incubation box upper cover 41 which is rotatably mounted on the upper end of the incubation box base 40, and three incubation boxes 42 which are arranged left and right between the incubation box base 40 and the incubation box upper cover 41, that is, the incubation box upper cover 41 can be flipped open to expose the incubation box 42 inside, which is used for absorbing the liquid inside or discharging the liquid after the gun tip 8 passes through, and a plurality of antibody incubation grooves 44 which are arranged left and right are recessed at the upper end of the incubation box 42, and at least three second through holes 43 which are arranged left and right and for the gun tip 71 to pass through are provided at the position corresponding to each incubation box on the incubation box upper cover 41, and each through hole corresponds to an antibody incubation groove 44; a second cooling box 45 and a second temperature conducting plate 46 which are arranged at the upper end of the second cooling box 45 are provided inside the incubation box base 40, and the second cooling box 45 is provided with a second heat conducting plate 46 which is provided at the upper end of the second cooling box 45. Used to cool down the incubation box 42. The incubation box 42 is arranged on the second heat conduction plate 46, which can better conduct the temperature to the liquid in the incubation box 42. The second cooling box 45 is provided with a water inlet and a water outlet, which is used to connect with an external water supply device to form a circulating water cooling structure; a heat insulation cotton 36 is provided between the second cooling box 45 and the incubation box base 40, which is used for heat insulation and reducing excessive loss of useless temperature. At the same time, after the incubation box cover 41 is closed, the loss of internal temperature can also be reduced, and the temperature balance in the incubation box can be better maintained; a temperature sensor is provided on the second heat conduction plate 46 to monitor the temperature change in real time to ensure the temperature control of the protein reagent inside the test kit, and the temperature can be as low as about 4°; a handle 38 is provided at the front end of the incubation box cover 41 to facilitate flipping open the incubation box cover 41.

[0059] In one embodiment, the incubation box 42 is a protein blotting membrane incubation / washing container. The incubation box 42 is preferably made of plastic injection molding such as PP or PC. The size is determined according to the size of the standard polyacrylamide gel electrophoresis tank and the actual application. There are 3 specifications, namely a 90*70mm incubation box with a single-grid antibody incubation tank, a 90*30mm incubation box with a double-grid antibody incubation tank, and a 90*16mm incubation box with an antibody incubation tank (the double grid is divided at a ratio of 1:2, and the three grids are divided at a ratio of 1:1:1). Other sizes and specifications are also possible. When three incubation boxes 42 are used, the three different specifications of incubation boxes can be randomly matched according to needs. They can be three incubation boxes of the same specification. The incubation box combination can also be a combination of incubation boxes of three different specifications, or a combination of incubation boxes of which two are of the same specification and the other is of a different specification, so there are a total of 27 different combinations to install the incubation boxes to meet different experimental needs; the three second vias 43 above each incubation box can just adapt to the above-mentioned three different specifications of incubation boxes. When a one-grid incubation box is used, only one of the second vias (preferably the middle second via) is used; when a two-grid incubation box is used, only the two second vias on the left and right sides are used, and these two vias correspond to one grid of antibody incubation tanks respectively; when a three-grid incubation box is used, the three vias just correspond to one grid of antibody incubation tanks respectively.

[0060] In one embodiment, the membrane washing and incubation belt refrigeration integrated machine also includes a camera 73 disposed above the incubation box assembly 4 and used for irradiating downwards, for photographing and identifying the combination of the incubation boxes below, so as to design the experimental process.

[0061] In one embodiment, a handle 421 extending from between the incubation box upper cover 41 and the incubation box base 40 is provided at one end of the incubation box 42, so that the incubation box 42 can be conveniently taken out by using the handle.

[0062] In one embodiment, if Figures 4 to 5 As shown, the rocking platform includes a base 74 arranged at the bottom of the cavity, a second motor 75 arranged on the base 74, and a crank-connecting rod assembly that transmission connects the second motor 75 and the incubation box base 40, the crank-connecting rod assembly includes a crank 18 fixed at one end to the output shaft of the second motor 75 and a connecting rod 19 rotatably connected to the other end of the crank 18 at one end, and the middle of the bottom rear side of the incubation box base 40 is rotatably connected to the other end of the connecting rod 19 through a connecting plate 47; the upper end of the base 74 is also symmetrically provided with two support seats 48 for supporting the front end of the incubation box assembly 4, and the bottom front side of the incubation box base 40 is provided with two support seats 48 for supporting the front end of the incubation box assembly 4 The support seats 48 correspond one to one with the connecting parts 49 that are rotatably connected, that is, under the drive of the second motor 75, the connecting rod 19 makes a reciprocating motion along the arc, driving the entire incubation box assembly to flip over repeatedly, that is, the incubation box base 40 is tilted and rotated up and down around the support seat 48. When the incubation box is swung to a horizontal state, it is convenient to install or remove the incubation box. At the same time, the second motor can use a stepping motor or add a sensor. When the incubation box assembly 4 tilts backward at the maximum angle, the liquid in the incubation box is tilted and concentrated to the rear end of the incubation box, which is convenient for the gun tip to absorb the internal reagent / waste liquid, utilizing the working principle of the crank connecting rod assembly.

[0063] In one embodiment, the reagent kit assembly 3 is arranged on the base 74 and is located on the left side of the incubation box assembly 4. One end of the base 74 is provided with a bottom box 76 located on the right side of the incubation box assembly. The bottom box 76 is provided with two recesses 77 for accommodating the cleaning liquid box 6 and the waste liquid box 5 respectively. The front end of the recess 77 is open, which is convenient for sliding the cleaning liquid box 6 and the waste liquid box 5 into or out of the cleaning liquid box 6 and the waste liquid box 5. The front end of the cleaning liquid box 6 and the waste liquid box 5 are both provided with a handle 38. When taking out, the handle 38 is pulled out by one hand. When most of the cleaning liquid box 6 or the waste liquid box 5 is out, the other hand holds it up to complete the removal process.

[0064] In one embodiment, the top surfaces of the reagent box assembly 3, the incubation box assembly 4, the waste liquid box 5 and the cleaning liquid box 6 are flush, so that the gun tip can be easily moved back and forth among them.

[0065] In one embodiment, if Fig.15As shown, the inner bottoms of the cleaning liquid box and the waste liquid box are both tilted backwards, so that the liquid is always concentrated at the rear end of the box, which is convenient for sucking and discharging the liquid cleanly.

[0066] In one embodiment, the rocking platform also includes a U-shaped sensor 191 located on one side of the connecting rod 19. When the incubation box is rocked to a horizontal position, the connecting rod can be detected by the U-shaped sensor 221. At this time, it can be set to the initial position to ensure that the incubation box assembly 4 is in a horizontal position.

[0067] In one embodiment, if Figures 6 to 9 As shown, a Y-axis driving device for driving the gun head frame 7 to move forward and backward is provided in the base 74, a sliding groove 51 extending forward and backward is provided on one side of the base 74, and the Y-axis driving device includes a sliding seat 52 provided in the base 74 and extending forward and backward along the base, a slider 53 slidingly provided on the sliding seat 52, a Y-axis screw rod 54 rotating on the sliding seat 52 through a bearing and extending forward and backward along the base, and a third motor 55 driving the Y-axis screw rod 54 to rotate, the slider 53 is threadedly connected with the Y-axis screw rod 54, and a supporting sheet 56 with one end extending out of the sliding groove 51 is provided on the slider 53, that is, the supporting sheet 56 can move forward and backward within the range of the sliding groove under the drive of the third motor 55, and the gun head frame 7 is located on the left side of the base 74 and fixed on the supporting sheet 56.

[0068] In one embodiment, if Figure 3 and Fig.17As shown, the reagents used in the experiment, such as blocking solution, primary antibody, and secondary antibody, are all proteins, which will slowly denature at room temperature. The entire experimental process lasts for several hours. If the protein reagents are not temperature-controlled, protein denaturation may affect the experimental results. At the same time, expensive reagents such as primary antibodies and secondary antibodies cannot be reused. Therefore, a cooling box for cooling is provided in the reagent kit assembly and the incubation box assembly. In order to supply coolant to the cooling box, the membrane washing and incubation belt refrigeration integrated machine also includes a water tank 10 arranged in the outer shell 1, a third cooling box 11 circulatedly connected to the water tank 10 through a pipeline, and a refrigerator 12 abutting against the bottom surface of the third cooling box 11. One end of the third cooling box 11 is provided with a water inlet and a water outlet respectively connected to the water tank 10, and the pipeline connecting the water inlet to the water tank 10 is provided with an internal circulation The pump 15 (preferably a water pump) forms a separate circulation loop between the third cooling box 11, the internal circulation pump 15 and the water tank 10, and the coolant (preferably alcohol or ethylene glycol coolant) in the water tank 10 can be pumped into the third cooling box 11 by the internal circulation pump 15, and the liquid entering the third cooling box 11 is cooled by the refrigerator 12. After cooling, the cooled liquid is discharged back into the water tank. At this time, the liquid in the water tank is cooled. After circulating cooling in this way, the temperature can be reduced to below zero; the first cooling box 34 and the second The water inlet and the water outlet of the cooling box 45 are respectively connected to the water tank 10 through pipelines, and the first water pump 16 is provided on the pipeline connecting the water tank 10 and the water inlet of the first cooling box 34. At this time, a separate circulation loop is formed between the first cooling box 34, the first water pump 16 and the water tank 10, and the liquid in the water tank 10 can be pumped into the first cooling box 34 by the first water pump 16, and the first cooling box 34 is circulated to cool down, and then the temperature of the corresponding reagent in the test kit can be reduced by temperature conduction; the pipeline connecting the water tank 10 and the water inlet of the second cooling box 45 A second water pump 17 is provided on it, and a separate circulation loop is formed between the second cooling box 45, the second water pump 17 and the water tank 10. The liquid in the water tank 10 can be pumped into the second cooling box 45 by the second water pump 17, and the second cooling box 45 is circulated to cool down, and then the temperature of the corresponding reagents in incubation boxes of different specifications can be reduced by temperature conduction; here, three independent circulation water circuits are formed between the water tank and the three cooling boxes, and the three circuits do not interfere with each other. One or two circuits can be opened for use separately, or all three circuits can be used together.

[0069] In one embodiment, if Fig.17 As shown, the water tank 10 is provided with a liquid level sensor 161 for detecting the liquid level and a temperature sensor 37 for detecting the temperature of the liquid in the water tank 10, which can read the liquid level and temperature in the water tank in real time. When the liquid in the water tank is insufficient, liquid can be added into the water tank to maintain the required cooling function.

[0070] In one embodiment, if Fig.16 As shown, the driving device includes a vertical driving device 78 for driving the pipette 72 to move longitudinally and a horizontal driving device 79 for driving the pipette 72 and the vertical driving device 78 to move horizontally; the vertical driving device 78 is provided with a first travel switch 80 for limiting the up and down travel of the pipette 72, and the left and right ends of the horizontal driving device 79 are provided with second travel switches 81 for limiting the left and right travel of the vertical driving device 79.

[0071] In one embodiment, both the vertical drive device and the horizontal drive device may adopt a screw drive mechanism, and may also adopt a belt drive mechanism or a cylinder drive mechanism, etc. The screw drive mechanism, belt drive mechanism and cylinder drive mechanism are all conventional drive mechanisms in the existing art and will not be described in detail here.

[0072] In one embodiment, if Figure 1-2 and Fig.18 As shown, a touch screen 95 for human-computer interaction and display is also provided on one side of the housing 1, and the touch screen 95 can be manually rotated in two levels. The first rotation is the rotation of the touch screen 95 in the horizontal direction, which is a switching process of the touch screen 95 rotating from the side to the front; the second rotation is the rotation of the touch screen 95 in the vertical direction, which realizes the upward rotation of the touch screen from a vertical state to an inclined or horizontal state; in order to achieve the purpose of two-level rotation, a rotating head 96 that rotates in the longitudinal direction is rotatably provided on one side of the housing 1, that is, the rotating head 96 can rotate in the vertical plane, and a rotating seat 97 that can rotate in the horizontal direction is rotatably provided at the lower end of the rotating head 96, that is, the rotating seat 97 can rotate in the horizontal plane, and the rear side of the touch screen 95 is fixed on the rotating seat, so that it can be rotated left and right and up and down to adjust the touch screen to a comfortable screen display viewing angle.

[0073] In other embodiments, the thermal insulation cotton is preferably made of polyurethane.

[0074] The technical solutions provided by the embodiments of the present invention are introduced in detail above. Specific examples are used in this article to illustrate the principles and implementation methods of the embodiments of the present invention. The description of the above embodiments is only applicable to help understand the principles of the embodiments of the present invention. At the same time, for those skilled in the art, according to the embodiments of the present invention, there will be changes in the specific implementation methods and application scopes. In summary, the content of this specification should not be understood as a limitation on the present invention.

Claims

1. A membrane washing and incubation machine with refrigeration, characterized in that: include: A shell having a cavity inside and an opening at the front side of the shell; A panel, which is closable and arranged at the front end of the housing and is used to cover or open the opening; A reagent kit assembly, which is arranged at the bottom of the chamber and is used to separate and accommodate a plurality of different reagents; An incubation box assembly, which is arranged on the right side of the test kit assembly, and a plurality of antibody incubation tanks are arranged on the incubation box assembly; A swing platform, which is arranged at the bottom of the cavity and is used to drive the incubation box assembly to tilt, rotate and swing forward and backward; A gun head rack, on which a plurality of gun heads are inserted, and the gun heads are mounted on one side of the swing platform and can move forward and backward along the swing platform; A waste liquid box, which is arranged on the right side of the incubation box assembly or the left side of the reagent kit assembly and is used to collect waste liquid; A cleaning liquid box, which is arranged on the right side or the left side of the waste liquid box and is used to contain the cleaning liquid; The pipetting assembly includes a pipette arranged at the upper end of the cavity and used to grab any gun tip on the gun tip rack, and a driving device used to drive the pipette to move in the longitudinal and horizontal directions, so that the gun tip can move back and forth between the gun tip rack, the reagent kit assembly, the incubation box assembly, the waste liquid box and the cleaning liquid box.

2. The membrane washing and incubation integrated machine with refrigeration as claimed in claim 1, characterized in that: The cam is an electric motor which is connected to the transmission gear of the motor, and the cam is connected to the transmission gear of the motor by a toothed wheel. The cam is connected to the transmission gear of the motor and the transmission gear of the motor is connected to the transmission gear of the motor. The cam is connected to the transmission gear of the motor and the transmission gear of the motor is connected to the transmission gear of the motor.

3. The membrane washing and incubation integrated machine with refrigeration as claimed in claim 1, characterized in that: The reagent kit assembly includes a reagent kit base, a reagent kit upper cover rotatably mounted on the upper end of the reagent kit base, and at least three reagent kits arranged between the reagent kit base and the reagent kit upper cover and used to respectively accommodate different reagents. The reagent kit upper cover is provided with at least one first through hole for a gun tip to pass through at a position corresponding to each reagent kit. A first cooling box and a first temperature conduction plate disposed on the upper end of the first cooling box are disposed inside the reagent kit base. The reagent kit is disposed on the first temperature conduction plate. A water inlet and a water outlet are disposed on the first cooling box. Insulation cotton is disposed between the first cooling box and the reagent kit base. A temperature sensor is disposed on the first temperature conduction plate. A handle is disposed on the reagent kit upper cover and a front end of the reagent kit.

4. The membrane washing and incubation integrated machine with refrigeration as claimed in claim 3, characterized in that: The incubation box assembly includes an incubation box base, an incubation box upper cover which is rotatably mounted on the upper end of the incubation box base, and at least three incubation boxes arranged left and right between the incubation box base and the incubation box upper cover, the upper end of the incubation box is recessed with at least one antibody incubation groove arranged left and right, and the incubation box upper cover is provided with at least three second through holes arranged left and right at the position corresponding to each incubation box for the gun tip to pass through; a second cooling box and a second heat conduction plate which is arranged at the upper end of the second cooling box are provided inside the incubation box base, the incubation box is provided on the second heat conduction plate, a water inlet and a water outlet are provided on the second cooling box, heat insulation cotton is provided between the second cooling box and the incubation box base, and a temperature sensor is provided on the second heat conduction plate; a handle is provided at the front end of the incubation box upper cover.

5. The membrane washing and incubation integrated machine with refrigeration as claimed in claim 4, characterized in that: It also includes a camera which is arranged above the incubation box assembly and is used for irradiating downwards.

6. The membrane washing and incubation integrated machine with refrigeration as claimed in claim 4, characterized in that: The swaying platform comprises a base arranged at the bottom of the cavity, a second motor arranged on the base, and a crank-connecting rod assembly which transmission connects the second motor and the incubation box base, the crank-connecting rod assembly comprising a crank at one end fixed to the output shaft of the second motor and a connecting rod at one end rotatably connected to the other end of the crank, the middle of the bottom rear side of the incubation box base is rotatably connected to the other end of the connecting rod through a connecting plate; the upper end of the base is also symmetrically provided with two support seats for supporting the front end of the incubation box assembly, the bottom front side of the incubation box base is provided with two connecting members which are rotatably connected to the support seats in a one-to-one correspondence; the test kit assembly is arranged on the base and is located on one side of the incubation box assembly, one end of the base is provided with a bottom box located on the other side of the incubation box assembly, the bottom box is concavely provided with two grooves for respectively accommodating the cleaning liquid box and the waste liquid box, and the front ends of the grooves are openings; the front ends of the cleaning liquid box and the waste liquid box are both provided with handles.

7. The membrane washing and incubation integrated machine with refrigeration as claimed in claim 6, characterized in that: The base is provided with a Y-axis driving device for driving the gun head rack to move forward and backward, and a sliding groove extending forward and backward along the base is opened on one side of the base. The Y-axis driving device includes a sliding seat provided in the base and extending forward and backward along the base, a slider slidably provided on the sliding seat, a Y-axis screw rod rotatably provided on the sliding seat and extending forward and backward along the base, and a third motor driving the Y-axis screw rod to rotate, the slider is threadedly connected with the Y-axis screw rod, the slider is provided with a supporting plate with one end extending out of the sliding groove, and the gun head rack is arranged on the supporting plate outside the base.

8. The membrane washing and incubation integrated machine with refrigeration as claimed in any one of claims 4 to 7, characterized in that: It also includes a water tank arranged in the outer shell, a third cooling box connected to the water tank for circulation through a pipeline, and a refrigerator abutting the surface of the third cooling box, one end of the third cooling box is provided with a water inlet and a water outlet connected to the water tank respectively, and an internal circulation pump is provided in the pipeline connecting the water inlet and the water tank; the water inlet and the water outlet of the first cooling box and the second cooling box are connected to the water tank through pipelines respectively, and a first water pump is provided on the pipeline connecting the water tank and the water inlet of the first cooling box, and a second water pump is provided on the pipeline connecting the water tank and the water inlet of the second cooling box; a liquid level sensor for detecting the liquid level and a temperature sensor for detecting the temperature of the liquid in the water tank are provided in the water tank.

9. The membrane washing and incubation integrated machine with refrigeration as claimed in claim 1, characterized in that: The driving device includes a vertical driving device for driving the pipette to move longitudinally and a horizontal driving device for driving the pipette and the vertical driving device to move horizontally; the vertical driving device is provided with a first travel switch for limiting the up and down travel of the pipette, and the left and right ends of the horizontal driving device are both provided with a second travel switch for limiting the left and right travel of the vertical driving device.

10. The membrane washing, incubation and refrigeration integrated machine according to claim 1, characterized in that: A touch screen for human-computer interaction and display is also provided on one side of the shell. A rotating head that rotates longitudinally is rotatably provided on one side of the shell, and a rotating seat that rotates horizontally is rotatably provided at the lower end of the rotating head. The rear side of the touch screen is fixed on the rotating seat.

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