Cell cryopreservation rack and matched cryopreservation box

Abstract

The utility model discloses a cell cryopreservation rack and a matched cryopreservation box, which have the beneficial effects that through the matched design of the cryopreservation rack and the cryopreservation box, not only can programmed cooling of cryopreserved cells be well realized, but also quick transfer of cryopreservation containers such as cell cryopreservation tubes and the like can be realized. Due to the design of the cryopreservation frame, the placement problem of the cryopreservation frame in the programmed cooling instrument is also simplified, and more placement frame specifications can be designed according to the inner cavity so as to meet the use requirements.

Description

Technical Field

[0001] The utility model relates to the technical field of medicine production, in particular to a cell freezing rack and a matching freezing box. Background Art

[0002] Cell cryopreservation is one of the main methods of cell preservation. Using cryopreservation technology to store cells in liquid nitrogen at -196℃ can temporarily remove cells from the growth state and preserve their cell characteristics. When needed, the cells can be revived for experiments. It can prevent cell loss due to contamination of the cells being cultured or other unexpected events, and play a role in cell preservation.

[0003] At present, programmed cooling boxes and programmed cooling instruments are widely used in cell cryopreservation in the biopharmaceutical industry. The temperature of the cell cryopreservation tubes after programmed cooling is between -196℃ and -140℃. In order to ensure the quality of cell cryopreservation and prevent the cryopreservation tubes from warming up during the transfer process, they need to be quickly transferred under conditions such as dry ice to maintain low temperatures. In order to achieve uniform cooling, each box of the programmed cooling box generally arranges about 20 cell cryopreservation tubes in a circle, and achieves gradient cooling through strict sealing. Since the programmed cooling box has a small number of cells and is tightly sealed, the cryopreserved cells are generally transferred manually. The programmed cooling instrument can accommodate 100-1000 cryopreservation tubes at the same time according to the needs of the cryopreservation scale. Due to the large number of cryopreservation tubes placed, how to quickly transfer the target cell cryopreservation tubes remains an urgent problem to be solved.

[0004] Although there are fully automatic cell freezing equipment that can achieve rapid cell transfer through robotic arms, this equipment is expensive and difficult to purchase and use in actual production processes. Currently, there is an urgent need for a cell freezing rack and matching freezing boxes that can achieve rapid transfer of cryopreservation containers such as cell cryopreservation tubes. Utility Model Content

[0005] In view of the deficiencies in the prior art, the technical problem to be solved by the utility model is to provide a cell freezing rack and a matching freezing box that are convenient for quickly transferring freezing containers such as cell freezing tubes.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0007] A cell freezing rack and a matching freezing box, comprising a freezing rack, a drawable bottom plate and a freezing box, wherein the freezing rack is provided with a plurality of freezing container placement positions, and the freezing container placement positions can together with the drawable bottom plate form a space for placing freezing containers; the freezing box is provided with a plurality of freezing container accommodating cavities, the number and arrangement of the freezing container accommodating cavities are the same as those of the freezing container placement positions, and the two are matched one-to-one with each other, and the freezing container accommodating cavities are used to accommodate freezing containers that fall from the freezing container placement positions when the drawable bottom plate is drawn out; in a use state, the freezing rack, the drawable bottom plate and the freezing box are respectively located at the upper, middle and lower positions, and when the drawable bottom plate is drawn out, the freezing container will fall from the freezing container placement position into the corresponding freezing container accommodating cavity in the freezing box.

[0008] Furthermore, double-layer slide rails are arranged on two opposite sides of the bottom of the freezing rack, the double-layer slide rails include an upper groove of the double-layer slide rail and a lower groove of the double-layer slide rail, and the pull-out bottom plate is combined with the freezing rack through the upper groove of the double-layer slide rail; single-layer slide rails are arranged on two opposite sides above the freezing box, and the freezing box and the freezing rack are combined by docking the single-layer slide rail with the lower groove of the double-layer slide rail.

[0009] Furthermore, the number of the freezing container placement positions is selected from 5x5, 9x9, and 10x10.

[0010] Furthermore, the freezing container is a freezing tube or a freezing bag.

[0011] Furthermore, the freezing rack is made of stainless steel strips or wire materials.

[0012] Furthermore, the freezing rack is a hollow rack.

[0013] Furthermore, the freezing box is made of low-temperature resistant material.

[0014] Furthermore, the freezing box is made of stainless steel or plastic.

[0015] Furthermore, the freezing rack can be stacked after being combined with the drawable bottom plate.

[0016] The utility model has the following advantages:

[0017] Compared with the prior art, the utility model has the following beneficial effects: the utility model can realize programmed cooling of cryopreserved cells and rapid transfer of cryopreservation containers such as cell cryopreservation tubes through the matching design of cell cryopreservation racks and cryopreservation boxes. At the same time, the design of the cell cryopreservation rack simplifies the placement of cryopreservation racks in the programmed cooling instrument, and more cell cryopreservation racks can be placed according to the inner cavity specifications to meet the use requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the structure of the cell freezing rack and the matching freezing box of the utility model;

[0019] Figure 2 This is a schematic diagram of the bottom of the cell freezing rack of the utility model;

[0020] Figure 3 It is a top view of the cell freezing rack of the utility model;

[0021] Figure 4 This is a front view of the cell freezing rack of the utility model;

[0022] Figure 5 It is a side view (left view and right view) of the cell freezing rack of the utility model;

[0023] Figure 6 It is a top view of the freezing box of the utility model;

[0024] Figure 7 It is a side view of the freezing box of the utility model.

[0025] In the figure, 1-freezing rack; 2-double-layer slide rail; 3-pull-out bottom plate; 4-single-layer slide rail; 5-freezing box; 21-upper groove of double-layer slide rail; 22-lower groove of double-layer slide rail. DETAILED DESCRIPTION

[0026] The present invention is further described below in conjunction with specific embodiments. It should be understood that the embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0027] Example

[0028] A cell freezing rack and a matching freezing box, such as Figure 1-7 As shown, it includes a freezing rack 1, a retractable bottom plate 3 and a freezing box 5. Double-layer slide rails 2 are arranged on opposite sides of the bottom of the freezing rack 1, and the double-layer slide rails 2 include a double-layer slide rail upper groove 21 and a double-layer slide rail lower groove 22. The retractable bottom plate 3 is combined with the freezing rack 1 through the double-layer slide rail upper groove 21; single-layer slide rails 4 are arranged on opposite sides above the freezing box 5, and the freezing box 5 is combined with the freezing rack 1 through the single-layer slide rail 4 and the double-layer slide rail lower groove 22. The freezing rack 1 is provided with a plurality of freezing container placement positions, and the freezing container placement positions can form a space for placing freezing containers together with the retractable bottom plate 3; the freezing box 5 is provided with a plurality of freezing container accommodating chambers, and the number and arrangement of the freezing container accommodating chambers are the same as those of the freezing container placement positions, and the two correspond to each other one by one; when the retractable bottom plate 3 is pulled out, the freezing container accommodating chamber is used to accommodate the freezing container dropped from the freezing container placement position.

[0029] In this embodiment, before the cells are subjected to programmed cooling, the removable bottom plate 3 should be inserted along the groove 21 on the double-layer slide rail at the bottom of the freezing rack 1 to seal the bottom of the freezing rack 1. The cell freezing containers are placed in the freezing rack 1 in sequence according to the number of frozen cells, and then the freezing rack 1 containing the cell freezing containers is placed in the programmed cooling instrument for freezing. After the cells are frozen, the freezing rack 1 is taken out of the programmed cooling instrument, and a matching freezing box 5 that can accommodate the same number of freezing containers is taken, and the combination is inserted along the groove 22 under the double-layer slide rail at the bottom of the freezing rack 1, and the removable bottom plate 3 is pulled out from one side, and the cell freezing containers all fall into the freezing box 5 at one time, and the freezing box 5 containing the cell freezing containers is placed in a liquid nitrogen tank for storage, thereby realizing the rapid transfer of a large number of frozen cells and effectively preventing the freezing containers from warming up.

[0030] The above disclosure is only the preferred embodiment of the utility model, and does not limit the utility model in other forms. Those skilled in the art can understand that the utility model is not limited to each specific implementation. For those skilled in the art, without departing from the technical principle of the utility model, it is possible to make improvements or modifications within the scope of the utility model, and the various technical features mentioned in various places in the specification can be combined with each other, and these improvements or modifications should be included in the protection scope of the utility model.

Claims

1. A cell freezing rack and a matching freezing box, characterized in that: The freezing rack (1) comprises a freezing rack (1), a removable bottom plate (3) and a freezing box (5), wherein the freezing rack (1) is provided with a plurality of freezing container placement positions, wherein the freezing container placement positions can together with the removable bottom plate (3) form a space for placing freezing containers; the freezing box (5) is provided with a plurality of freezing container accommodating cavities, wherein the number and arrangement of the freezing container accommodating cavities are the same as those of the freezing container placement positions, and the two are matched one to one, and the freezing container accommodating cavities are used to accommodate freezing containers that fall from the freezing container placement positions when the removable bottom plate (3) is withdrawn; In the use state, the freezing rack (1), the removable bottom plate (3) and the freezing box (5) are located at the upper, middle and lower positions respectively. When the removable bottom plate (3) is pulled out, the freezing container will fall from the freezing container placement position into the corresponding freezing container accommodating cavity in the freezing box (5).

2. The cell freezing rack and the matching freezing box according to claim 1, characterized in that: The bottom of the freezing rack (1) is provided with double-layer slide rails (2) on two opposite sides, the double-layer slide rails (2) include a double-layer slide rail upper groove (21) and a double-layer slide rail lower groove (22), and the removable bottom plate (3) is combined with the freezing rack (1) through the double-layer slide rail upper groove (21); the freezing box (5) is provided with single-layer slide rails (4) on two opposite sides above, and the freezing box (5) and the freezing rack (1) are combined by docking the single-layer slide rails (4) with the double-layer slide rail lower groove (22).

3. The cell freezing rack and the matching freezing box according to claim 1, characterized in that: The number of the freezing container placement positions is selected from 5x5, 9x9, and 10x10.

4. The cell freezing rack and the matching freezing box according to claim 1, characterized in that: Cryogenic containers include cryotubes or cryobags.

5. The cell freezing rack and the matching freezing box according to claim 1, characterized in that: The freezing rack (1) is made of stainless steel strips or wire materials.

6. The cell freezing rack and the matching freezing box according to claim 1, characterized in that: The freezing storage rack (1) is a hollow frame.

7. The cell freezing rack and the matching freezing box according to claim 1, characterized in that: The freezing box (5) is made of stainless steel or plastic.

8. The cell freezing rack and the matching freezing box according to claim 1, characterized in that: The freezing rack (1) and the drawable bottom plate (3) can be stacked after being combined.

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