Centrifugal tube capable of realizing filtration

Abstract

The centrifugal tube comprises a tube body, a first cover body, a filter screen and a second cover body, the filter screen is integrated in the first cover body, the complicated step that an external cell filter is additionally erected at an opening of a traditional centrifugal tube is abandoned, filtering and centrifugal preparation processes are integrated, the operation process is greatly simplified, and the operation efficiency is improved. Manpower and time investment are effectively saved; the hidden danger of sample residue leakage when the external cell filter is taken away is thoroughly eliminated, and the laboratory environment is powerfully prevented from being polluted; compared with the prior art which depends on self-weight filtering of a sample, the centrifugal tube has the advantages that the second cover body covers the first cover body, so that the sample is limited in the first cover body, when the filtering efficiency needs to be improved, the centrifugal tube can be placed in a centrifugal machine, the filtering process is accelerated by virtue of centrifugal force, and the filtering efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of centrifuge tube technology, and in particular to a centrifuge tube that can achieve filtration. Background Technology

[0002] The primary function of centrifuge tubes is to hold various samples during centrifugation, using the centrifugal force generated by the high-speed rotation of the centrifuge to separate different components within the sample. In existing techniques, to filter adherent cells, digested tissues, or processed tumor masses to obtain single cells, a cell filter is first placed at the opening of the centrifuge tube. The adherent cells, digested tissues, or processed tumor masses are then added to the cell filter and allowed to flow into the centrifuge tube by gravity. After filtration, the cell filter is removed, and the centrifuge tube is sealed before further centrifugation. However, removing the cell filter before sealing the centrifuge tube is cumbersome and inconvenient. Furthermore, during the removal of the cell filter, residual sample on the filter can easily scatter onto the lab bench, instrument surfaces, and other surrounding environments, causing contamination. Utility Model Content

[0003] In view of this, the present invention proposes a centrifuge tube that can achieve filtration, with the aim of improving the ease of operation and avoiding contamination when removing the cell filter.

[0004] The solution provided by this utility model includes:

[0005] A centrifuge tube capable of filtration, comprising:

[0006] The tube body has an opening at its top;

[0007] The first cover has a first connecting part at its first end that is detachably connected to the tube body, and a second connecting part at its second end. The end face of the second connecting part is recessed with a first receiving cavity. The inner bottom surface of the first receiving cavity is provided with a first through hole. The first through hole penetrates the first cover and communicates with the interior of the tube body.

[0008] A filter screen is disposed within the first accommodating cavity and covers the first perforation;

[0009] A second cover is detachably connected to a second connecting portion of the first cover to seal the first accommodating cavity.

[0010] As a further optional solution, a fixing member is also included. The fixing member is disposed in the first accommodating cavity, and a second accommodating cavity is recessed at its first end face. A pressing part is formed at its second end. A second through hole is formed on the inner bottom surface of the second accommodating cavity, and the second through hole communicates with the first through hole. The pressing part presses the filter screen onto the inner bottom surface of the first accommodating cavity. The fixing member and the first accommodating cavity are interference-fitted.

[0011] As a further optional solution, a flexible first connecting piece is provided between the tube body and the first cover body, and the tube body, the first connecting piece and the first cover body are an integral plastic part.

[0012] As a further optional solution, a flexible second connecting piece is provided between the first cover and the second cover, and the tube, the first connecting piece, the first cover, the second connecting piece and the second cover are an integral plastic part.

[0013] As a further optional solution, the first connecting part of the first cover is snap-fitted to the tube body.

[0014] As a further optional solution, the first connecting part is in the shape of a cylindrical tube and is inserted into the opening; one of the outer peripheral wall of the first connecting part and the inner wall of the tube body is provided with an annular first retaining strip, and the other is provided with an annular first retaining groove; the first retaining strip and the first retaining groove are snapped together, and the first retaining strip and the first retaining groove are sealed and abutted together.

[0015] As a further optional solution, the first cover is provided with a first lifting plate.

[0016] As a further optional solution, the second connecting part of the first cover is connected to the second cover by a snap-fit ​​connection.

[0017] As a further optional solution, the second connecting part is in the shape of a cylindrical tube, and the second cover is recessed with a third receiving cavity, into which the second connecting part is inserted; one of the outer peripheral wall of the second connecting part and the inner wall of the third receiving cavity is provided with an annular second retaining strip, and the other is provided with an annular second retaining groove; the second retaining strip and the second retaining groove are snapped together, and the second retaining strip and the second retaining groove are sealed and abutted together.

[0018] As a further optional solution, the second cover is provided with a second opening plate.

[0019] Compared with the prior art, the centrifuge tube capable of filtration described in this application has at least the following advantages:

[0020] 1. The filter screen is integrated into the first cavity of the first cover, eliminating the complicated steps of setting up an external cell filter at the opening of the centrifuge tube. The filtration and centrifugation preparation process is integrated, which greatly simplifies the operation process and effectively saves manpower and time.

[0021] 2. The filter screen is fixed inside the first cover, which completely eliminates the risk of sample leakage when the external cell filter is removed, and effectively protects the laboratory environment from contamination;

[0022] 3. Unlike existing technologies that rely on sample weight for filtration, this application uses a second cover to cover the first cover, so that the sample is confined within the first cover. When it is necessary to improve filtration efficiency, the centrifuge tube of this application can be placed in a centrifuge to accelerate the filtration process and improve filtration efficiency by using centrifugal force. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the structure of a centrifuge tube capable of filtration according to Embodiment 1 of this utility model;

[0024] Figure 2 This is a cross-sectional schematic diagram of a centrifuge tube capable of filtration according to Embodiment 1 of this utility model;

[0025] Figure 3 This is an exploded schematic diagram of a centrifuge tube capable of filtration according to Embodiment 2 of this utility model;

[0026] Figure 4 This is an exploded cross-sectional view of a centrifuge tube capable of filtration according to Embodiment 2 of this utility model;

[0027] Figure 5 This is a cross-sectional schematic diagram of a centrifuge tube capable of filtration according to Embodiment 2 of this utility model:

[0028] Figure 6 This is an exploded schematic diagram of a centrifuge tube capable of filtration according to Embodiment 3 of this utility model;

[0029] Figure 7 This is a schematic diagram of the structure of a centrifuge tube capable of filtration according to Embodiment 3 of this utility model;

[0030] Figure 8 This is an exploded schematic diagram of a centrifuge tube capable of filtration according to Embodiment 4 of this utility model;

[0031] In the diagram: 1. Tube body; 11. Opening; 12. First slot;

[0032] 2. First cover; 21. First connecting part; 211. First retaining strip; 22. Second connecting part; 221. First receiving cavity; 222. Second retaining strip; 23. First perforation; 24. First opening plate;

[0033] 3. Second cover; 31. Third receiving cavity; 311. Second slot; 32. Second hinged plate;

[0034] 4. Filter screen;

[0035] 5. Fixing element; 51. Second receiving cavity; 52. Pressing part; 53. Second through hole;

[0036] 6. First connecting piece;

[0037] 7. Second connecting piece. Detailed Implementation

[0038] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit its scope.

[0039] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "vertical", "horizontal", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0040] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0041] In this utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0042] Example 1

[0043] like Figure 1 , Figure 2 and Figure 3 As shown in the figure, this embodiment illustrates a centrifuge tube capable of filtration, including a tube body 1, a first cover 2, a filter screen 4, and a second cover 3. The top of the tube body 1 has an opening 11. The first end of the first cover 2 has a first connecting portion 21 detachably connected to the tube body 1, and the second end has a second connecting portion 22. The end face of the second connecting portion 22 has a recessed first receiving cavity 221. The inner bottom surface of the first receiving cavity 221 has a first through hole 23, which penetrates the first cover 2 and communicates with the interior of the tube body 1. The filter screen 4 is disposed in the first receiving cavity 221 and covers the first through hole 23. The second cover 3 is detachably connected to the second connecting portion 22 of the first cover 2 to seal the first receiving cavity 221.

[0044] The filter screen 4 can be fixed in the first accommodating cavity 221 by means of welding, bonding, ultrasonic welding, etc. The first connecting part 21 and the tube body 1, and the second connecting part 22 and the second cover 3 can be connected by conventional detachable connection methods such as snap-fit ​​connection and threaded connection, as long as the first cover 2 and the tube body 1 are sealed and connected and the second cover 3 and the first cover 2 are sealed.

[0045] When applying, such as Figure 2 As shown, first open the second cover 3 to expose the first accommodating cavity 221, then add the sample into the first accommodating cavity 221. The sample is filtered through the filter screen 4 and flows into the tube 1. Finally, close the second cover 3 back onto the first cover 2, and then proceed with the subsequent centrifugation separation process.

[0046] In this embodiment, the filter 4 is integrated into the first accommodating cavity 221 of the first cover 2, eliminating the complicated steps of setting up an external cell filter at the centrifuge tube opening 11 in the traditional way, integrating the filtration and centrifugation preparation process, greatly simplifying the operation process, and effectively saving manpower and time investment.

[0047] In this embodiment, the filter 4 is fixed inside the first cover 2, which completely eliminates the risk of sample leakage when the external cell filter is removed, and effectively protects the laboratory environment from pollution.

[0048] In this embodiment, unlike existing technologies that rely on sample weight for filtration, a second cover 3 is placed over a first cover 2 to confine the sample within the first cover 2. When higher filtration efficiency is required, the centrifuge tubes of this embodiment can be placed in a centrifuge to accelerate the filtration process and improve efficiency through centrifugal force. Of course, this embodiment can also rely solely on sample weight for filtration.

[0049] Furthermore, the centrifuge tube of this embodiment can also be used in other ways. For example, a tissue sample can be placed into the tube body 1 first, then the first cap 2 can be installed on the tube body 1, and the second cap 3 can be installed on the first cap 2. Then, it can be placed in a tissue dissociator to dissociate and release the cells in the tissue sample. After that, the second cap 3 can be opened, and the tube body 1 with the first cap 2 installed can be inverted. The cells are filtered and separated by the filter 4 on the first cap 2, and the remaining tissue sample after the cells are released is retained in the tube body 1. Compared with the prior art, this embodiment, based on the integrated structure of the first cap 2 and the filter 4, can improve the ease of use.

[0050] Example 2

[0051] like Figure 3-5 As shown in the figure, Embodiment 2 illustrates a centrifuge tube capable of filtration, comprising a tube body 1, a first cover 2, a filter screen 4, a second cover 3, and a fixing member 5. Embodiment 2 adds the fixing member 5 compared to Embodiment 1.

[0052] The fixing member 5 is disposed in the first accommodating cavity 221. The end face of the first end of the fixing member 5 is recessed to form a second accommodating cavity 51, and the second end of the fixing member 5 is formed with a pressing part 52. The inner bottom surface of the second accommodating cavity 51 is provided with a second through hole 53, which communicates with the first through hole 23. The pressing part 52 presses the filter screen 4 onto the inner bottom surface of the first accommodating cavity 221. The fixing member 5 and the first accommodating cavity 221 are in an interference fit.

[0053] Specifically, during assembly, the filter screen 4 is first laid on the inner bottom surface of the first accommodating cavity 221, and then the fixing member 5 is pressed into the first accommodating cavity 221, so that the filter screen 4 is clamped between the fixing member 5 and the inner bottom surface of the first accommodating cavity 221, thereby fixing the filter screen 4. The above structure makes the installation and fixing of the filter screen 4 simpler, which facilitates the improvement of the production efficiency of the centrifuge tube and reduces the production difficulty.

[0054] The fastener 5 is interference-fitted with the first accommodating cavity 221, which makes the fastener 5 less prone to loosening and ensures the stable fixation of the filter screen 4.

[0055] In this embodiment, the pressing part 52 is the end face of the fixing member 5; in other embodiments, the pressing part 52 may also be a silicone pad provided on the end face of the fixing member 5, so that the pressing part 52 and the filter screen 4 are tightly pressed together, ensuring that the sample cannot be wrapped around the filter screen 4 from the gap between the fixing member 5 and the filter screen 4.

[0056] In this embodiment, to improve the assembly and disassembly speed of the first cover 2, the first connecting part 21 of the first cover 2 is snap-fitted to the tube body 1. Specifically, as shown... Figure 4 and Figure 5As shown, the first connecting part 21 is in the shape of a cylindrical tube and is inserted into the opening 11; one of the outer peripheral wall of the first connecting part 21 and the inner wall of the tube body 1 is provided with an annular first retaining strip 211, and the other is provided with an annular first retaining groove 12; the first retaining strip 211 and the first retaining groove 12 are snapped together, and the first retaining strip 211 and the first retaining groove 12 are sealed and abutted together.

[0057] Furthermore, to improve the speed of assembling and disassembling the second cover 3, the second connecting part 22 of the first cover 2 is snap-fitted to the second cover 3. Specifically, as shown... Figure 4 and Figure 5 As shown, the second connecting part 22 is in the shape of a cylindrical tube, and the second cover 3 is recessed with a third receiving cavity 31. The second connecting part 22 is inserted into the third receiving cavity 31. One of the outer peripheral wall of the second connecting part 22 and the inner wall of the third receiving cavity 31 is provided with an annular second retaining strip 222, and the other is provided with an annular second retaining groove 311. The second retaining strip 222 and the second retaining groove 311 are snapped together, and the second retaining strip 222 and the second retaining groove 311 are sealed and abutted together.

[0058] In addition, for ease of operation, such as Figure 3 and Figure 5 As shown, the first cover 2 is provided with a first lifting plate 24, and the second cover 3 is provided with a second lifting plate 32. Thus, during operation, the first lifting plate 24 can be pried open to lift the first cover 2; similarly, the second lifting plate 32 can be pried open to lift the second cover 3.

[0059] Example 3

[0060] like Figure 6 and Figure 7 As shown, in Embodiment 3, a first connecting piece 6 is added based on Embodiment 2. Specifically, a flexible first connecting piece 6 is provided between the tube body 1 and the first cover body 2. The tube body 1, the first connecting piece 6, and the first cover body 2 are an integral plastic part.

[0061] This reduces the number of parts and prevents the first cover 2 from being easily lost after it is opened.

[0062] Example 4

[0063] like Figure 8 As shown, in Embodiment 4, a second connecting piece 7 is added based on Embodiment 3. Specifically, a flexible second connecting piece 7 is provided between the first cover 2 and the second cover 3. The tube 1, the first connecting piece 6, the first cover 2, the second connecting piece 7, and the second cover 3 are an integral plastic part.

[0064] Thus, Embodiment 4 further reduces the number of parts compared to Embodiment 3. When opening the second cover 3 to add samples, it prevents the second cover 3 from being easily lost, and avoids the need to place the second cover 3 on a test bench or other location, thus preventing the second cover 3 from being contaminated and affecting the test results of subsequent samples.

[0065] In the above embodiments, the descriptions of each embodiment have different focuses. For parts not described in detail in a certain embodiment, please refer to the relevant descriptions of other embodiments.

[0066] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and substitutions can be made without departing from the technical principles of the present utility model, and these improvements and substitutions should also be considered within the protection scope of the present utility model.

Claims

1. A filterable centrifuge tube, comprising: The centrifuge tube comprises: a tube body, a top of which is provided with an opening; a first cover body, a first end of which is provided with a first connecting part detachably connected with the tube body, and a second end of which is provided with a second connecting part, an end surface of the second connecting part being recessed with a first accommodating cavity, an inner bottom surface of the first accommodating cavity being provided with a first through hole, the first through hole penetrating through the first cover body and being communicated with an inside of the tube body; a filter screen, which is arranged in the first accommodating cavity and covers the first through hole; a second cover body, which is detachably connected with the second connecting part of the first cover body to seal the first accommodating cavity.

2. The centrifuge tube capable of filtering according to claim 1, further comprising a fixing member, which is arranged in the first accommodating cavity, an end surface of a first end of the fixing member being recessed with a second accommodating cavity, an inner bottom surface of the second accommodating cavity being provided with a second through hole, the second through hole being communicated with the first through hole, and a press-fit part being formed at a second end of the fixing member, the press-fit part pressing the filter screen on the inner bottom surface of the first accommodating cavity, and the fixing member and the first accommodating cavity being in interference fit.

3. The centrifuge tube capable of filtering according to claim 2, wherein a first connecting piece which is bendable is arranged between the tube body and the first cover body, and the tube body, the first connecting piece and the first cover body are integrated plastic members.

4. The centrifuge tube capable of filtering according to claim 3, wherein a second connecting piece which is bendable is arranged between the first cover body and the second cover body, and the tube body, the first connecting piece, the first cover body, the second connecting piece and the second cover body are integrated plastic members.

5. The centrifuge tube capable of filtering according to any one of claims 1-4, wherein the first connecting part of the first cover body is snap-connected with the tube body.

6. The centrifuge tube capable of filtering according to claim 5, wherein the first connecting part is in the shape of a circular tube, the first connecting part is inserted into the opening, one of an outer peripheral wall of the first connecting part and an inner wall of the tube body is provided with a first clamping strip in the shape of a ring, and the other is provided with a first clamping groove in the shape of a ring, the first clamping strip and the first clamping groove are snap-connected, and the first clamping strip and the first clamping groove are in sealed abutment.

7. The centrifuge tube capable of filtering according to claim 6, wherein a first uncovering plate is arranged on the first cover body.

8. The centrifuge tube capable of filtering according to any one of claims 1-4, wherein the second connecting part of the first cover body is snap-connected with the second cover body.

9. The centrifuge tube capable of filtering according to claim 8, wherein the second connecting part is in the shape of a circular tube, a third accommodating cavity is recessed on the second cover body, the second connecting part is inserted into the third accommodating cavity, one of an outer peripheral wall of the second connecting part and an inner wall of the third accommodating cavity is provided with a second clamping strip in the shape of a ring, and the other is provided with a second clamping groove in the shape of a ring, the second clamping strip and the second clamping groove are snap-connected, and the second clamping strip and the second clamping groove are in sealed abutment. ​ ​ ​ ​ ​ ​ ​ ​ 10. The filterable centrifuge tube of claim 9, wherein: the second cover is provided with a second tab.

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