Cell treatment consumable pipe group and cell treatment equipment

Abstract

The utility model provides a cell processing consumable pipe group and cell processing equipment, the cell processing consumable pipe group comprises a centrifugal container, a magnetic sorting device, a first pipeline communicated between the centrifugal container and the magnetic sorting device, and a second pipeline communicated between the magnetic sorting device and a waste liquid container and arranged on a first driving pump, the culture flowing pipeline is communicated between the culture solution container and the first communication point; the first communication point is a point, between the first driving pump and the waste liquid container, on the second pipeline. According to the utility model, the pollution of cells is avoided, and meanwhile, the cell treatment efficiency is improved.

Description

Technical Field

[0001] This utility model belongs to the field of cell processing technology, and in particular relates to a cell processing consumable tube assembly and cell processing equipment. Background Technology

[0002] Currently, in the field of cell therapy, cell processing generally involves multiple steps, such as PBMC (peripheral blood mononuclear cell) isolation, magnetic bead incubation, cell sorting, cell harvesting, and cell culture. In existing technologies, each of these steps requires operators to change different equipment or tubing separately, resulting in cumbersome processes and low efficiency. Furthermore, changing equipment can easily lead to sample contamination. Summary of the Invention

[0003] This invention addresses the technical problems of low cell processing efficiency and easy contamination in the prior art by providing a cell processing consumable tube assembly and cell processing equipment.

[0004] In view of the above technical problems, this utility model provides a cell processing consumable tubing assembly. The cell processing consumable tubing assembly is in a sterile environment and includes a centrifuge container, a magnetic separation device, a first pipeline connecting the centrifuge container and the magnetic separation device, a second pipeline connecting the magnetic separation device and a waste liquid container and placed between them and a first drive pump, and a culture flow pipeline connecting a culture medium container and a first connection point. The first connection point is a point on the second pipeline located between the first drive pump and the waste liquid container.

[0005] Optionally, the cell processing consumable tubing assembly further includes an incubation end-product tubing connecting the incubation end-product container and a second connection point; the second connection point is a point on the second tubing located between the first drive pump and the waste liquid container.

[0006] Optionally, the cell processing consumable tubing assembly further includes a third tubing connected between the first tubing and the third connection point and located at the second drive pump, and a reagent inlet tubing connected between the additional reagent container and the fourth connection point; the third connection point is a point on the second tubing located between the first drive pump and the first connection point, and the fourth connection point is a point on the second tubing located between the first drive pump and the first connection point.

[0007] Optionally, the additional reagent container is provided with an external input port for introducing cleaning solution or magnetic bead reagent; the external input port is connected to the reagent introduction pipeline through the additional reagent container.

[0008] Optionally, the cell processing consumable tubing assembly further includes a fourth tubing connecting the centrifuge container and the fifth connection point and located at the third drive pump, and a preparation tubing connecting the sixth connection point and the preparation container; the fifth connection point is a point on the second tubing located between the second connection point and the waste liquid container, and the sixth connection point is a point on the second tubing located between the fifth connection point and the waste liquid container.

[0009] Optionally, the cell processing consumable tubing assembly further includes a gas-liquid separation device disposed on the formulation tubing.

[0010] Optionally, the cell treatment consumable tubing assembly further includes a fifth tubing connecting the seventh and eighth connection points; the seventh and eighth connection points are points on the fourth tubing located at opposite ends of the third drive pump.

[0011] Optionally, the cell processing consumable tubing assembly further includes a sixth tubing connecting the ninth and tenth connection points; the ninth connection point is the point on the fourth tubing located between the third drive pump and the eighth connection point; the tenth connection point is the point on the first tubing located between the eleventh connection point and the centrifuge container; and the eleventh connection point is the connection point between the first tubing and the third tubing.

[0012] Optionally, the cell processing consumable tubing assembly further includes a sample tubing connecting the sample container and the twelfth connection point; the twelfth connection point is a point on the second tubing located between the fifth connection point and the waste liquid container;

[0013] or / and

[0014] The cell treatment consumable tubing assembly also includes an intermediate product tubing connecting the intermediate product container and the thirteenth connection point; the thirteenth connection point is a point on the second tubing located between the fifth connection point and the waste liquid container;

[0015] or / and

[0016] The cell processing consumable tubing assembly also includes a buffer tubing connecting the buffer container and the fourteenth connection point; the fourteenth connection point is a point on the second tubing located between the fifth connection point and the first drive pump;

[0017] or / and

[0018] The cell processing consumable tubing also includes a gas input line connected to the first line, a one-way valve disposed on the gas input line, and a sterile filter disposed at the end of the gas input line away from the first line.

[0019] A cell processing device includes a first drive pump, a first switching valve, a second switching valve for controlling the on / off state of the culture flow pipeline, and the cell processing consumable tubing assembly.

[0020] The second pipeline is installed on the first switch valve, which is located between the first connection point and the waste liquid container; the culture flow pipeline is installed on the second switch valve; the second pipeline is installed on the first drive pump, which is located between the first connection point and the magnetic separation device.

[0021] The cell processing consumable tubing assembly provided by this utility model includes a centrifuge container, a magnetic separation device, a first pipeline connecting the centrifuge container and the magnetic separation device, a second pipeline connecting the magnetic separation device and a waste liquid container and placed between the magnetic separation device and a first drive pump, and a culture flow pipeline connecting a culture medium container and a first connection point; the first connection point is a point on the second pipeline located between the first drive pump and the waste liquid container. The cell processing consumable tubing assembly provided by this invention can generate or eliminate a magnetic field within the magnetic sorting device. After generating a magnetic field within the magnetic sorting device, peripheral blood mononuclear cell solution containing magnetically labeled cells from a centrifuge container can be input into the magnetic sorting device through a first tubing to adsorb the magnetically labeled cells. The remaining peripheral blood mononuclear cell solution is then input into a waste container through a second tubing. Subsequently, the magnetic field is eliminated within the magnetic sorting device, and culture medium solution from a culture medium container is input into the magnetic sorting device through a culture flow tubing connected to the second tubing. This rinses the magnetically labeled cells that are no longer adsorbed by the magnetic field within the magnetic sorting device, and the rinsed mixture is then transported back to the centrifuge container through the first tubing for cell culture. In this way, multiple processes can be sequentially completed within the cell processing consumable tubing assembly, eliminating the need to change equipment after each process to proceed to the next, thus avoiding cell contamination and improving cell processing efficiency. Attached Figure Description

[0022] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0023] Figure 1 This is a schematic diagram of the structure of a cell processing consumable tube assembly provided in one embodiment of the present invention.

[0024] Figure 2 This is a schematic diagram of the structure of a cell processing device provided in one embodiment of the present invention.

[0025] The reference numerals in the accompanying drawings are as follows:

[0026] 110. Centrifuge container; 120. Waste liquid container; 130. Culture medium container; 140. Incubation final product container; 150. Additional reagent container; 151. External input port; 160. Formulation container; 170. Sample container; 180. Intermediate product container; 190. Buffer container; 200. Magnetic separator; 301. First tubing; 302. Second tubing; 303. Culture flow tubing; 304. Incubation final product tubing; 305. Third tubing; 306. Reagent inlet tubing; 307. Fourth tubing; 3 08. Formulation tubing; 309. Fifth tubing; 310. Sixth tubing; 311. Sample tubing; 312. Intermediate product tubing; 313. Buffer tubing; 314. Gas input tubing; 315. Check valve; 316. Sterile filter; 410. First drive pump; 420. Second drive pump; 430. Third drive pump; 500. Gas-liquid separator; 600. Switch valve; 610. First switch valve; 620. Second switch valve; 700. Tube clamp; 800. Bubble sensor; 900. Pressure sensor. Detailed Implementation

[0027] To make the technical problems solved, technical solutions, and beneficial effects of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain this utility model and are not intended to limit this utility model.

[0028] like Figure 1 As shown, one embodiment of this utility model provides a cell processing consumable tubing assembly. The cell processing consumable tubing assembly is in a sterile environment and includes a centrifuge container 110, a magnetic separation device 200, a first tubing 301 connecting the centrifuge container 110 and the magnetic separation device 200, a second tubing 302 connecting the magnetic separation device 200 and a waste liquid container 120 and placed in a first drive pump 410, and a culture flow tubing 303 connecting a culture medium container 130 and a first connection point; the first connection point is a point on the second tubing 302 located between the first drive pump 410 and the waste liquid container 120.

[0029] Understandably, the centrifuge container 110, the magnetic sorting device 200, the first pipeline 301, the second pipeline 302, and the culture flow pipeline 303 are connected by sealing at their interconnections to maintain a sterile environment within the cell processing consumable tubing assembly. The magnetic sorting device 200 can generate or eliminate its surrounding magnetic field using a magnetic field generating device (not shown). After generating a magnetic field within the magnetic sorting device 200, it can magnetically sort the flowing liquid (i.e., magnetically adsorb magnetically labeled cells into the magnetic sorting device 200 under the influence of an external magnetic field). The cell processing consumable tubing assembly can be equipped with features such as... Figure 2 The components shown include a switching valve 600 for controlling the on / off state of the pipeline, a pipe clamp 700 for controlling the on / off state of the pipeline, a bubble sensor 800 for detecting the fluid flow state in the pipeline, a pressure sensor 900 for detecting the fluid pressure in the pipeline, and a drive pump for controlling the flow of fluid in the cell processing consumable tubing assembly, thereby realizing cell processing. The second pipeline 302 can be a single, integral pipeline of the same diameter; the second pipeline 302 can also be a single or segmented pipeline with different diameters, so that the diameter of the section where the first drive pump 410 is located is set according to the parameters of the first drive pump 410 to match the first drive pump 410. The connection methods between the above-mentioned pipelines, centrifuge container 110 and magnetic separation device 200 include, but are not limited to, one or more of the following: integral connection or multi-way connector connection. The connection point at the first connection point can be achieved by setting a tee connector or other means to connect the second pipeline 302 and the culture flow pipeline 303. In the following embodiments, the connection points can all be achieved by using tee connectors or multi-way connectors to connect the pipelines.

[0030] In this embodiment, the centrifuge container 110 can store peripheral blood mononuclear cell fluid containing magnetically labeled cells, as well as a mixture of culture medium and magnetically labeled cells. The second conduit 302 and the culture flow conduit 303 can be respectively equipped with... Figure 2 The switch valve 600 shown is used to control the opening and closing of the pipeline, so that the peripheral blood mononuclear cell fluid remaining after being sorted by the magnetic sorting device 200 can be input into the waste liquid container 120 through the second pipeline 302, or the culture medium solution in the culture medium container 130 can be output to the magnetic sorting device 200 through the culture flow pipeline 303, depending on the actual situation.

[0031] In this embodiment, a magnetic field can be generated within the magnetic sorting device 200 first; the first drive pump 410 can be controlled to drive the peripheral blood mononuclear cell fluid containing magnetically labeled cells in the centrifuge container 110 to be input into the magnetic sorting device 200 through the first pipeline 301, so that the magnetically labeled cells in the flowing peripheral blood mononuclear cell fluid can be adsorbed into the magnetic sorting device 200 by the magnetic field, and the remaining peripheral blood mononuclear cell fluid can be input into the waste liquid container 120 through the second pipeline 302. After the bubble sensor 800 installed on the second pipeline 302 confirms that the peripheral blood mononuclear cell fluid containing magnetically labeled cells in the centrifuge container 110 has been transferred, the magnetic sorting device 200 is controlled to eliminate the magnetic field. The first drive pump 410 is controlled to drive the culture medium in the culture medium container 130 to be input into the magnetic sorting device 200 through the culture flow pipeline 303 and the second pipeline 302. The culture medium is used to rinse the magnetically labeled cells in the magnetic sorting device 200 that are no longer attracted by the magnetic field. The rinsed mixture is then transported to the centrifuge container 110 through the first pipeline 301 for cell culture. Thus, the sorting and culture of magnetically labeled cells are achieved without changing the equipment.

[0032] The cell processing consumable tubing provided by this utility model can generate or eliminate a magnetic field within the magnetic sorting device 200. After generating a magnetic field within the magnetic sorting device 200, peripheral blood mononuclear cell fluid containing magnetically labeled cells in the centrifuge container 110 can be input into the magnetic sorting device 200 through the first pipe 301 to adsorb the magnetically labeled cells in the magnetic sorting device 200. The remaining peripheral blood mononuclear cell fluid can be input into the waste liquid container 120 through the second pipe 302. After eliminating the magnetic field within the magnetic sorting device 200, culture medium solution in the culture medium container 130 can be input into the magnetic sorting device 200 through the culture flow pipe 303 connected to the second pipe 302 to rinse the magnetically labeled cells in the magnetic sorting device 200 that are no longer adsorbed by the magnetic field. The rinsed mixture can be transported to the centrifuge container 110 through the first pipe 301 for cell culture. In this way, multiple processes can be completed sequentially within the cell processing consumables tubing during cell processing, eliminating the need to change equipment after each process to proceed to the next, thus avoiding cell contamination and improving cell processing efficiency.

[0033] like Figure 2As shown, in one embodiment, the cell processing consumable assembly further includes a gas input line 314 connected to the first line 301, a one-way valve 315 disposed on the gas input line 314, and a sterile filter 316 disposed at the end of the gas input line away from the first line 301. It is understood that the gas input line 314 can be used to input sterile gas during cell culture in the centrifuge container 110. The sterile filter 316, located at the air interface, ensures a sterile environment within the cell processing consumable assembly.

[0034] like Figure 2 As shown, in one embodiment, the cell processing consumable tubing assembly further includes an incubation end-product tubing 304 connecting the incubation end-product container 140 and a second connection point; the second connection point is a point on the second tubing 302 located between the first drive pump 410 and the waste liquid container 120. It is understood that the second connection point and the first connection point can be the same connection point or different connection points. In one embodiment, the magnetic sorting device 200, the first drive pump 410, the second connection point, the first connection point, and the waste liquid container 120 are sequentially connected to the second tubing 302. The incubation end-product tubing 304 can be configured as follows... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, so that the incubation end product solution without magnetically labeled cells remaining after being sorted by the magnetic sorting device 200 can be input into the incubation end product container 140 through the incubation end product pipeline 304, according to the actual situation.

[0035] In this embodiment, the magnetic separation device 200 can be controlled to generate a magnetic field, and the first drive pump 410 can be controlled to drive the incubation final product solution obtained after cell culture in the centrifuge container 110 to be input into the magnetic separation device 200 through the first pipeline 301. The magnetic magnetic field will adsorb the magnetically labeled cells in the incubation final product solution flowing through it into the magnetic separation device 200. The remaining incubation final product solution without magnetically labeled cells will be output to the incubation final product container 140 through the second pipeline 302 and the incubation final product pipeline 304 in sequence. Thus, the collection of the incubation final product solution is further realized without replacing the equipment.

[0036] like Figure 2As shown, in one embodiment, the cell processing consumable tubing assembly further includes a third tubing 305 connecting the first tubing 301 and the third connection point and positioned within the second drive pump 420, and a reagent inlet tubing 306 connecting the additional reagent container 150 and the fourth connection point; the third connection point is a point on the second tubing 302 located between the first drive pump 410 and the first connection point, and the fourth connection point is a point on the second tubing 302 located between the first drive pump 410 and the first connection point. It is understood that the additional reagent container 150 can be used to hold excess peripheral blood mononuclear cell fluid. The third connection point, the fourth connection point, and the second connection point can all or partly be the same connection point, or they can be different connection points. In one embodiment, the third connection point, the fourth connection point, and the second connection point can be interconnected in any order on the second pipeline 302 to form a first connection group; the magnetic separation device 200, the first drive pump 410, the first connection group, the first connection point, and the waste liquid container 120 are sequentially connected on the second pipeline 302.

[0037] like Figure 2 As shown, in one embodiment, the additional reagent container 150 is provided with an external input port 151 for introducing cleaning solution or magnetic bead reagent; the external input port 151 is connected to the reagent introduction line 306 through the additional reagent container 150. It can be understood that the additional reagent container 150 can also introduce cleaning solution or magnetic bead reagent through the external input port 151, thereby holding the cleaning solution or magnetic bead reagent.

[0038] In this embodiment, the second drive pump 420 can be controlled to drive the incubation end product solution without magnetically labeled cells in the incubation end product container 140 to bypass the magnetic separation device 200 and instead sequentially enter the centrifuge container 110 through the incubation end product pipeline 304, the third pipeline 305, and the first pipeline 301, thereby avoiding interference from the magnetic separation device 200 on the incubation end product solution without magnetically labeled cells. Subsequently, the second drive pump 420 can be controlled to drive the cleaning solution in the additional reagent container 150 to sequentially enter the centrifuge container 110 through the reagent introduction pipeline 306, the third pipeline 305, and the first pipeline 301, so that the incubation end product solution is centrifuged and cleaned in the centrifuge container 110, thereby further realizing the centrifugal cleaning of the incubation end product solution without replacing the equipment.

[0039] like Figure 2As shown, in one embodiment, the cell processing consumable tubing group further includes a fourth tubing 307 connecting the centrifuge container 110 and the fifth connection point and located in the third drive pump 430, and a preparation tubing 308 connecting the sixth connection point and the preparation container 160; the fifth connection point is a point on the second tubing 302 located between the second connection point and the waste liquid container 120, and the sixth connection point is a point on the second tubing 302 located between the fifth connection point and the waste liquid container 120. It is understood that the fifth connection point, the sixth connection point, and the first connection point can all or partly be the same connection point, or they can be different connection points. In one embodiment, the first connection point, the fifth connection point, and the sixth connection point can be interconnected in any order on the second tubing 302 to form a second connection group; the magnetic separation device 200, the first drive pump 410, the first connection group, the second connection group, and the waste liquid container 120 are sequentially connected on the second tubing 302. The preparation tubing 308 can be provided with... Figure 2 The switch valve shown is used to control the on / off of the pipeline, so that the preparation in the centrifuge container 110 can be input into the preparation container 160 through the preparation pipeline 308 according to the actual situation.

[0040] In this embodiment, the third drive pump 430 can be controlled to drive the incubation final product liquid after centrifugation and cleaning in the centrifuge container 110 to be sequentially input into the formulation container 160 through the fourth pipeline 307, the second pipeline 302 and the formulation pipeline 308, and to harvest and dispense the incubation final product liquid, thereby further realizing the harvesting and dispensing of the incubation final product liquid without changing the equipment.

[0041] like Figure 2 As shown, in one embodiment, the cell processing consumable tubing assembly further includes a gas-liquid separator 500 disposed on the formulation tubing 308. Understandably, the gas-liquid separator 500 is used to filter out impurities such as coagulated clumps and bubbles from the liquid. The gas-liquid separator 500 includes, but is not limited to, a cell filter membrane for filtering coagulated clumps from the liquid and a dropper for filtering bubbles, connected in sequence. The gas-liquid separator 500 disposed on the formulation tubing 308 can filter impurities in the formulation collected in the formulation container 160.

[0042] like Figure 2As shown, in one embodiment, the cell treatment consumable tubing group further includes a fifth tubing 309 connecting the seventh and eighth connecting points; the seventh and eighth connecting points are points on the fourth tubing 307 located at opposite ends of the third drive pump 430. It is understood that the seventh and fifth connecting points can be the same connecting point, thereby allowing the fifth tubing 309 to directly connect to the second tubing 302. Alternatively, the seventh and fifth connecting points can be different connecting points, allowing the fifth tubing 309 to connect to the second tubing 302 via the fourth tubing 307. The seventh connecting point can be a connecting point in the second connecting group, interconnected with the first, fifth, and sixth connecting points on the second tubing 302 in any order. The fifth tubing 309 can be configured as follows... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, so that the liquid can be selected to flow through the third drive pump 430 or through the fifth pipeline 309 according to the actual situation.

[0043] like Figure 2 As shown, in one embodiment, the cell processing consumable tubing assembly further includes a sixth tubing 310 connecting the ninth and tenth connection points; the ninth connection point is a point on the fourth tubing 307 located between the third drive pump 430 and the eighth connection point; the tenth connection point is a point on the first tubing 301 located between the eleventh connection point and the centrifuge container 110; the eleventh connection point is a connection point between the first tubing 301 and the third tubing 305. It is understood that the ninth connection point and the eighth connection point can be the same connection point or different connection points. In one embodiment, the third drive pump 430, the ninth connection point, the eighth connection point, and the centrifuge container 110 are sequentially connected to the fourth tubing 307. The tenth and eleventh connection points can be the same connection point or different connection points. In one embodiment, the centrifuge container 110, the tenth connection point, and the eleventh connection point are sequentially connected to the first tubing 301. The sixth tubing 310 can be configured as follows... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, thereby making the selection of the flow path of liquid between the first drive pump 410, the second drive pump 420, the fifth pipeline 309 and the sixth pipeline 310 more flexible, thus realizing a variety of different functions.

[0044] like Figure 2As shown, in one embodiment, the cell processing consumable tubing assembly further includes a sample tubing 311 connecting the sample container 170 and the twelfth connection point; the twelfth connection point is a point on the second tubing 302 located between the fifth connection point and the waste liquid container 120. It can be understood that the twelfth connection point can be a connection point in the second connection group, interconnected with the first connection point, the fifth connection point, the sixth connection point, and the seventh connection point in any order on the second tubing 302. The sample tubing 311 can be configured as follows... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, so that the sample liquid in the centrifuge container 110 can be input into the sample container 170 through the sample pipeline 311 according to the actual situation.

[0045] In this embodiment, the third drive pump 430 or the second drive pump 420 can be controlled to drive the sample liquid in the sample container 170 into the centrifuge container 110; then, the centrifuge container 110 is controlled to centrifuge the sample liquid in the centrifuge container 110, thereby further realizing the centrifugation operation of the sample liquid without changing the equipment.

[0046] like Figure 2 As shown, in one embodiment, the cell treatment consumable tubing assembly further includes an intermediate product line 312 connecting the intermediate product container 180 and the thirteenth connection point; the thirteenth connection point is a point on the second line 302 located between the fifth connection point and the waste liquid container 120. It can be understood that the thirteenth connection point can be a connection point in the second connection group, interconnected with the first connection point, the fifth connection point, the sixth connection point, the seventh connection point, and the twelfth connection point in any order on the second line 302, as long as a connection is provided between the second connection group and the waste liquid container 120. Figure 2 The switch valve shown is used to control the on / off state of the pipeline, allowing selection of whether the liquid in the second pipeline 302 is input into the waste liquid container 120 via the second pipeline 302 according to actual conditions. The intermediate product pipeline 312 can be equipped with... Figure 2 The switch valve shown is used to control the on / off state of the pipeline, so that the peripheral blood mononuclear cell fluid in the intermediate product container 180 can be input into the centrifuge container 110 through the intermediate product pipeline 312 as needed.

[0047] In this embodiment, the third drive pump 430 or the second drive pump 420 can be controlled to drive the peripheral blood mononuclear cell fluid in the centrifuge container 110 into the intermediate product container 180; and the remaining waste liquid in the centrifuge container 110 can be controlled to be sent into a waste liquid bag, thereby further realizing the screening of peripheral blood mononuclear cell fluid without changing the equipment. Then, the third drive pump 430 or the second drive pump 420 is controlled to drive the peripheral blood mononuclear cell fluid in the intermediate product container 180 into the centrifuge container 110, and the centrifuge container 110 is controlled to perform a centrifugation operation, thereby further realizing the centrifugation operation of peripheral blood mononuclear cell fluid without changing the equipment. Finally, during the centrifugation operation in the centrifuge container 110, the washing solution in the additional reagent container 150 is continuously input into the centrifuge container 110 to wash the peripheral blood mononuclear cell fluid in the centrifuge container 110 in a continuous flow manner, thereby further realizing the continuous washing of peripheral blood mononuclear cell fluid without changing the equipment.

[0048] like Figure 2 As shown, in one embodiment, the cell treatment consumable tubing assembly further includes a buffer conduit 313 connecting the buffer container 190 and the fourteenth connection point; the fourteenth connection point is a point on the second conduit 302 located between the fifth connection point and the first drive pump 410. It can be understood that the fourteenth connection point can be a connection point in the first connection group, interconnected with the second connection point, the third connection point, and the fourth connection point in any order on the second conduit 302. The buffer conduit 313 can be configured as follows: Figure 2 The switch valve shown is used to control the opening and closing of the pipeline, so that the buffer solution in the buffer container 190 can be input into the centrifuge container 110 through the buffer pipeline 313 according to the actual situation.

[0049] like Figure 2As shown, one embodiment of this utility model also provides a cell processing device, including a first drive pump 410, a first switching valve 610, a second switching valve 620 for controlling the on / off state of the culture flow pipeline 303, and the cell processing consumable tubing assembly; the second pipeline 302 is installed on the first switching valve 610, and the first switching valve 610 is located between the first connection point and the waste liquid container 120; the culture flow pipeline 303 is installed on the second switching valve 620; the second pipeline 302 is installed on the first drive pump 410, and the first drive pump 410 is located between the first connection point and the magnetic sorting device 200. Understandably, the first drive pump 410 is used to drive the liquid flow in the second pipeline 302. The first drive pump 410 includes, but is not limited to, a peristaltic pump, which can drive the liquid flow without contacting the liquid, that is, by using rollers to peristaltically move the liquid in the pipeline, thus achieving non-contact liquid flow without contaminating the liquid, keeping the entire consumable tubing assembly in a sterile environment. The first drive pump 410 can also be other types of liquid pumps, as long as they can achieve the effect of driving liquid flow.

[0050] The cell processing equipment provided by this utility model can generate or eliminate a magnetic field within the magnetic separation device 200. After generating a magnetic field within the magnetic separation device 200, peripheral blood mononuclear cell fluid containing magnetically labeled cells in the centrifuge container 110 can be input into the magnetic separation device 200 through the first pipeline 301 to adsorb the magnetically labeled cells in the magnetic separation device 200. The remaining peripheral blood mononuclear cell fluid can be input into the waste liquid container 120 through the second pipeline 302. After eliminating the magnetic field within the magnetic separation device 200, culture medium solution in the culture medium container 130 can be input into the magnetic separation device 200 through the culture flow pipeline 303 connected to the second pipeline 302 to rinse the magnetically labeled cells in the magnetic separation device 200 that are no longer adsorbed by the magnetic field. The rinsed mixture can be transported to the centrifuge container 110 through the first pipeline 301 for cell culture. In this way, multiple processes can be completed sequentially within the cell processing consumables tubing during cell processing, eliminating the need to change equipment after each process to proceed to the next, thus avoiding cell contamination and improving cell processing efficiency.

[0051] The above are merely embodiments of the cell processing consumable tubing and cell processing equipment of this utility model, and are not intended to limit this utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A cell processing consumable tube set, comprising: The cell processing consumable tube group is in a sterile environment, including a centrifugal container, a magnetic separation device, a first pipeline connected between the centrifugal container and the magnetic separation device, a second pipeline connected between the magnetic separation device and a waste liquid container and arranged at a first driving pump, and a culture flow pipeline connected between a culture liquid container and a first connection point; the first connection point is a point on the second pipeline between the first driving pump and the waste liquid container.

2. The cell processing consumables tube set of claim 1, wherein, The cell processing consumable tube group further comprises an incubation end product pipeline connected between an incubation end product container and a second connection point; the second connection point is a point on the second pipeline between the first driving pump and the waste liquid container.

3. The cell processing consumables tube set of claim 1, wherein, The cell processing consumable tube group further comprises a third pipeline connected between the first pipeline and a third connection point and arranged at a second driving pump, and a reagent introduction pipeline connected between an additional reagent container and a fourth connection point; The third connection point is a point on the second pipeline between the first driving pump and the first connection point, and the fourth connection point is a point on the second pipeline between the first driving pump and the first connection point.

4. The cell processing consumables tube set of claim 3, wherein, The additional reagent container is provided with an external input port for inputting a cleaning liquid or a magnetic bead reagent; the external input port is connected to the reagent introduction pipeline through the additional reagent container.

5. The cell processing consumables tube set of claim 3, wherein, The cell processing consumable tube group further comprises a fourth pipeline connected between the centrifugal container and a fifth connection point and arranged at a third driving pump, and a preparation pipeline connected between a sixth connection point and a preparation container; the fifth connection point is a point on the second pipeline between the second connection point and the waste liquid container, the sixth connection point is a point on the second pipeline between the fifth connection point and the waste liquid container; and the second connection point is a point on the second pipeline between the first driving pump and the waste liquid container.

6. The cell processing consumables tube set of claim 5, wherein, The cell processing consumable tube group further comprises a gas-liquid separation device arranged on the preparation pipeline.

7. The cell processing consumables tube set of claim 5, wherein, The cell processing consumable tube group further comprises a fifth pipeline connected between a seventh connection point and an eighth connection point; the seventh connection point and the eighth connection point are points on the fourth pipeline respectively located at opposite ends of the third driving pump.

8. The cell processing consumables tube set of claim 7, wherein, The cell processing consumable tube group further comprises a sixth pipeline connected between a ninth connection point and a tenth connection point; the ninth connection point is a point on the fourth pipeline between the third driving pump and the eighth connection point; The tenth connection point is a point on the first pipeline between an eleventh connection point and the centrifugal container; the eleventh connection point is a connection point between the first pipeline and the third pipeline.

9. The cell processing consumables tube set of claim 5, wherein, The cell processing consumable tube group further comprises a sample pipeline connected between a sample container and a twelfth connection point; the twelfth connection point is a point on the second pipeline between the fifth connection point and the waste liquid container; Or / and The cell processing consumable tube group further comprises an intermediate product pipeline connected between an intermediate product container and a thirteenth connection point; the thirteenth connection point is a point on the second pipeline between the fifth connection point and the waste liquid container; Or / and The cell processing consumable tube set further comprises a buffer tube line communicated between the buffer container and a fourteenth communication point; the fourteenth communication point is a point on the second tube line between the fifth communication point and the first driving pump; Or / and The cell processing consumable tube set further comprises a gas input tube line communicated on the first tube line, a one-way valve arranged on the gas input tube line, and a sterile filter arranged at an end of the gas input tube line away from the first tube line.

10. A cell processing device, characterized by The cell processing consumable tube set comprises a first driving pump, a first switch valve, a second switch valve for controlling the on-off of the culture flow tube line, and the cell processing consumable tube set as claimed in any one of claims 1 to 9; The second tube line is arranged on the first switch valve, and the first switch valve is located between the first communication point and the waste liquid container; the culture flow tube line is arranged on the second switch valve; The second tube line is arranged on the first driving pump, and the first driving pump is located between the first communication point and the magnetic sorting device.

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