Nutrient solution slow-release device for porphyra germplasm preservation

Abstract

The utility model discloses a kind of nutrient solution slow-release devices for laver germplasm preservation, hollow filtering inner column is loaded in pipe body, micropore filter membrane is set on the bottom through-hole of hollow filtering inner column, and agar slow-release layer is set on micropore filter membrane;Sealing cover is set on the upper end of pipe body.The utility model is sterilely preserved: 0.22µm filter membrane completely blocks microbial contamination, prolongs germplasm survival time;Nutrition is controllable: filter membrane and agar layer design realizes nutrient solution stable release, avoids concentration fluctuation;It is easy to operate: modular structure is convenient to disassemble, clean and reuse.

Description

Technical Field

[0001] This utility model relates to a slow-release nutrient solution device for preserving laver germplasm. Background Technology

[0002] Existing methods for preserving laver germplasm typically employ liquid-phase (as described in the invention patent CN 103907526 B) or solid-phase sealed preservation, which suffer from problems such as difficulty in replenishing nutrient solution, uneven nutrient consumption, susceptibility to contamination, and cumbersome operation. This invention addresses these issues by designing a closed-loop filtration and cultivation device, combining microporous filtration and agar slow-release layer technology, to allow for the slow dripping of PES nutrient solution, achieving sterile and long-term stable preservation of laver germplasm. Utility Model Content

[0003] The purpose of this invention is to provide a slow-release nutrient solution device for preserving laver germplasm. By integrating a nutrient solution supply system and a sterile filtration structure, it solves the problems of high risk of contamination and unstable nutrient supply in traditional preservation methods.

[0004] The technical solution of this utility model is:

[0005] A slow-release nutrient solution device for preserving laver germplasm is characterized by: including a tube body that can stand upright, containing a hollow filter inner column, with a microporous filter membrane disposed on the bottom through-hole of the hollow filter inner column, and an agar layer disposed on the microporous filter membrane; and a sealing cap disposed at the upper end of the tube body.

[0006] The upper end of the hollow filter inner column is in the form of an outwardly extending ring, and the extension ring is engaged with the upper end of the tube body; the sealing cap covers the extension ring and is engaged with the extension ring.

[0007] The microporous filter membrane has a diameter of 0.22 µm, an agar concentration of 1%, an agar layer height of 0.5–1.5 cm, and an inner diameter of 2–2.5 cm for the hollow filter inner column.

[0008] Advantages of this utility model

[0009] 1. Aseptic preservation: The 0.22 µm filter membrane completely blocks microbial contamination and prolongs the survival time of germplasm.

[0010] 2. Controllable nutrition: The filter membrane and agar layer (i.e., slow-release layer) are designed to ensure stable release of nutrient solution and avoid concentration fluctuations.

[0011] 3. Easy to operate: The modular structure makes it easy to disassemble, clean and reuse. Attached Figure Description

[0012] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0013] Figure 1This is a structural schematic diagram of one embodiment of the present invention. Detailed Implementation

[0014] A slow-release nutrient solution device for preserving laver germplasm includes a tube body 1, a hollow filter inner column 2 inside the tube body, a 0.22 µm microporous filter membrane 3 disposed on the bottom through hole of the hollow filter inner column, an agar layer 4 disposed on the microporous filter membrane, a sealing cap 5 disposed at the upper end of the tube body, and an extension ring 6 extending outward at the upper end of the hollow filter inner column, which engages with the upper end of the tube body; the sealing cap 5 covers the extension ring 6 and engages with the extension ring.

[0015] The lid is made of a transparent material (such as polypropylene) and has a snap-fit ​​on the inside to seal it to the inner filter column and prevent external contamination.

[0016] The filter inner column is a hollow cylinder filled with PES nutrient solution. A 0.22 µm microporous membrane is located at the bottom, allowing the nutrient solution to slowly permeate into the column while blocking bacteria and fungi. A slow-release layer (such as agar) is placed above the membrane to further regulate the nutrient solution release rate.

[0017] The main body of the tube is made of transparent glass or corrosion-resistant plastic and is used to hold laver germplasm (free filaments).

[0018] Connection

[0019] 1. Cap and tube body: They are tightly connected by snaps, and a silicone sealing ring ensures that the inside of the device is sterile.

[0020] 2. Filter inner column and tube body: The filter inner column is tightly connected to the tube body by a snap-fit, and its filter membrane is connected to the inner cavity of the tube body. The nutrient solution slowly diffuses to the bottom of the tube body through the filter membrane.

[0021] Work process

[0022] 1. Assembly:

[0023] Place the laver seed at the bottom of the tube and add sterile seawater; install the filter inner column at the top of the tube and inject sterile PES nutrient solution into the filter inner column.

[0024] Tighten the lid to form a closed system.

[0025] 2. Preservation and Nutritional Supply:

[0026] The nutrient solution slowly permeates into the tube through a 0.22 µm filter membrane and an agar layer, providing continuous nutrients to the laver germplasm. An agar layer height of 0.5–1.5 cm is used, along with a hollow filter inner column with an inner diameter of 2–2.5 cm, allowing 0.5–1 ml of nutrient solution to permeate into the tube daily.

[0027] 3. Maintenance and Sampling:

[0028] Regularly open the lid to check the composition of the PES nutrient solution or add fresh PES nutrient solution.

[0029] Replace the filter inner column or filter membrane to maintain a sterile state in the long term.

Claims

1. A slow-release nutrient solution device for preserving laver germplasm, characterized in that: It includes a tube body, inside which is a hollow filter inner column. A microporous filter membrane is installed on the bottom through hole of the hollow filter inner column, and an agar layer is installed on the microporous filter membrane. A sealing cap is installed at the top of the tube body.

2. The slow-release nutrient solution device for preserving laver germplasm according to claim 1, characterized in that: The upper end of the hollow filter inner column is in the form of an outwardly extending ring, and the extension ring is engaged with the upper end of the tube body; the sealing cap covers the extension ring and is engaged with the extension ring.

3. A slow-release nutrient solution device for preserving laver germplasm according to claim 1 or 2, characterized in that: The microporous filter membrane has a diameter of 0.22 µm; the agar concentration is 1%; the agar layer height is 0.5–1.5 cm; and the inner diameter of the hollow filter inner column is 2–2.5 cm.

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