Multi-enzyme cascade catalytic reaction kettle

Abstract

The utility model relates to the field of bioengineering technology discloses a kind of multi-enzyme cascade catalytic reaction kettle, including inner tank, the outer wall of the inner tank is fixedly connected with heating mechanism, the outer wall of the heating mechanism is fixedly connected with outer tank, the top of the inner tank is fixedly connected with reaction mechanism, the top of the reaction mechanism is fixedly connected with grading mechanism, the reaction mechanism includes connecting ring, the bottom of the connecting ring is fixed in the top of inner tank, the outer wall of the connecting ring is fixedly connected with fixed column, the top of the connecting ring is fixedly connected with top cover.In the utility model, when using, start motor one, its output end drives rotating shaft one rotation, and then make rotating shaft one drive fan blade rotation, inner tank outer wall is equipped with heating pipe, for heating raw material in inner tank, inner tank top is connected with connecting ring, and is fixed by fixed column to prevent seepage, buffer tank top is connected with guide pipe, fuel can be added through guide pipe.

Description

Technical Field

[0001] This utility model relates to the field of bioengineering technology, and in particular to a multi-enzyme cascade catalytic reaction vessel. Background Technology

[0002] Against the backdrop of high energy consumption, pollution, and poor selectivity in traditional chemical synthesis, and the surging demand for high-value-added, chiral compounds, multi-enzyme cascade catalysis technology has emerged. It mimics the complex metabolic network within cells, linking multiple enzymes in series to achieve efficient conversion of multi-step chemical reactions under mild conditions. With its advantages of being green, precise, and low-consumption, it has opened up new pathways for the synthesis and processing of compounds in fields such as medicine, food, and energy, becoming a highly promising research hotspot and development direction in the field of biomanufacturing.

[0003] Multi-enzyme cascade catalytic reactors can shorten synthesis processes that originally required several days and multiple batches to be completed within hours. Currently, multi-enzyme cascade reactions can be carried out in mild environments, typically at near-normal temperature and pressure, ensuring enzyme activity and smooth reaction progress without the need for harsh conditions such as high temperature and high pressure. This not only reduces the pressure and heat resistance requirements of the equipment but also reduces energy consumption and safety hazards. Regarding the operational process, many existing reactors are not convenient enough in terms of feeding, sampling, and reaction process monitoring. Traditional reactors may require pausing the reaction, opening the sealing device, and manually adding substrate or enzyme solution, which not only easily introduces impurities and interferes with the reaction but also interrupts the reaction continuity, increasing operation time and cost. Furthermore, when sampling and monitoring the reaction progress, offline analysis of the reaction solution is often the only option, making real-time, online monitoring impossible. This makes it difficult to adjust parameters in a timely manner based on the actual reaction situation, potentially missing the optimal reaction control window. Utility Model Content

[0004] To overcome the above shortcomings, this invention provides a multi-enzyme cascade catalytic reactor, which aims to improve the problem of cumbersome multiple catalysis in the existing technology.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a multi-enzyme cascade catalytic reactor, comprising an inner tank, a heating mechanism fixedly connected to the outer wall of the inner tank, an outer tank fixedly connected to the outer wall of the heating mechanism, a reaction mechanism fixedly connected to the top of the inner tank, a grading mechanism fixedly connected to the top of the reaction mechanism, the reaction mechanism comprising a connecting ring, the bottom of the connecting ring fixedly fixed to the top of the inner tank, a fixing column fixedly connected to the outer wall of the connecting ring, a top cover fixedly connected to the top of the connecting ring, an outer shell fixedly connected to the top of the top cover, a motor fixedly connected to the inner wall of the outer shell, a rotating shaft rotatably connected to the output end of the motor, a rotating assembly fixedly connected to the outer wall of the rotating shaft, and a feeding assembly fixedly connected to the outer wall of the inner tank.

[0006] As a further description of the above technical solution:

[0007] The grading mechanism includes a processing tank, the bottom of which is fixedly connected to a top cover. A second rotating shaft is fixedly connected to the outer wall of the processing tank. A closing assembly is rotatably connected to the outer wall of the second rotating shaft. A second motor is fixedly connected to the outer wall of the processing tank. A stirring blade is rotatably connected to the output end of the second motor. A locking assembly is fixedly connected to the right outer wall of the processing tank. An indicator is fixedly connected to the top rear side of the first rotating shaft. A docking assembly is fixedly connected to the top of the top cover.

[0008] As a further description of the above technical solution:

[0009] The heating mechanism includes a heating tube, which is fixed to the outer wall of the inner tank, and a drain pipe is connected to the bottom of the heating tube.

[0010] As a further description of the above technical solution:

[0011] The rotating assembly includes fan blades, the inner wall of which is fixed to a rotating shaft, and a bottom shaft is fixedly connected to the bottom of the rotating shaft.

[0012] As a further description of the above technical solution:

[0013] The feeding assembly includes a buffer tank, which is disposed on the outer wall of the inner tank, and a guide tube is fixedly connected to the top of the buffer tank.

[0014] As a further description of the above technical solution:

[0015] The closing assembly includes a can lid, which is rotatably connected to a rotating shaft, and a handle is fixedly connected to the top of the can lid.

[0016] As a further description of the above technical solution:

[0017] The locking assembly includes a locking plate, which is fixed to the outer wall of the processing tank, and an L-shaped plate is slidably connected to the outer wall of the locking plate.

[0018] As a further description of the above technical solution:

[0019] The docking assembly includes a flange, the bottom of which is connected to the top cover, and the top of which is fixedly connected to a docking interface.

[0020] This utility model has the following beneficial effects:

[0021] 1. In this utility model, when in use, the motor is started, and its output end drives the rotating shaft to rotate, which in turn drives the fan blade to rotate. The outer wall of the inner tank is provided with a heating tube for heating the raw materials in the inner tank. The top of the inner tank is connected to a connecting ring and fixed by a fixing column to prevent leakage. The outer wall of the inner tank is connected to a buffer tank, and the top of the buffer tank is connected to a guide pipe, through which fuel can be added.

[0022] 2. In this utility model, a processing tank is provided on the right side of the top cover. When processing various raw materials, slide the L-shaped plate along the positioning plate, hold the handle, rotate along the second rotating shaft to open the tank cover, put the raw materials into the processing tank, start the second motor, and its output end drives the stirring blade to rotate. After the initial processing is completed, open the gate at the bottom of the processing tank, and the raw materials are introduced into the connecting ring for secondary reaction. Attached Figure Description

[0023] Figure 1 This is a front perspective view of a multi-enzyme cascade catalytic reactor proposed in this utility model;

[0024] Figure 2 This is a partial structural exploded view of a multi-enzyme cascade catalytic reactor proposed in this utility model;

[0025] Figure 3 This is a partial structural diagram of a multi-enzyme cascade catalytic reactor proposed in this utility model;

[0026] Figure 4 This is a partial structural illustration of a multi-enzyme cascade catalytic reactor proposed in this utility model;

[0027] Figure 5 This is a partial structural schematic diagram of a multi-enzyme cascade catalytic reactor proposed in this utility model.

[0028] Legend:

[0029] 1. Inner tank; 2. Reaction mechanism; 201. Connecting ring; 202. Fixing column; 203. Top cover; 204. Outer shell; 205. Motor 1; 206. Rotating shaft; 207. Rotating assembly; 2071. Fan blade; 2072. Bottom shaft; 208. Feeding assembly; 2081. Buffer tank; 2082. Guide pipe; 3. Grading mechanism; 301. Processing tank; 302. Rotating shaft; 303. Closing assembly; 3031. Tank cover; 3032. Handle; 304. Motor 2; 305. Stirring blade; 306. Locking assembly; 3061. Locking plate; 3062. L-shaped plate; 307. Indicator; 308. Docking assembly; 3081. Flange; 3082. Docking interface; 4. Heating mechanism; 401. Heating tube; 402. Unblocking pipe; 5. Outer tank. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] Please see the appendix Figure 1 - Appendix Figure 3 This utility model provides an embodiment of a multi-enzyme cascade catalytic reactor, comprising an inner tank 1, a heating mechanism 4 fixedly connected to the outer wall of the inner tank 1, an outer tank 5 fixedly connected to the outer wall of the heating mechanism 4, a reaction mechanism 2 fixedly connected to the top of the inner tank 1, a grading mechanism 3 fixedly connected to the top of the reaction mechanism 2, the reaction mechanism 2 including a connecting ring 201, the bottom of the connecting ring 201 fixed to the top of the inner tank 1, a fixing column 202 fixedly connected to the outer wall of the connecting ring 201, a top cover 203 fixedly connected to the top of the connecting ring 201, an outer shell 204 fixedly connected to the top of the top cover 203, and a motor 205 fixedly connected to the inner wall of the outer shell 204. The output end of motor 205 is rotatably connected to a rotating shaft 206. The outer wall of the rotating shaft 206 is fixedly connected to a rotating component 207. The outer wall of the inner tank 1 is fixedly connected to a feeding component 208. The top of the connecting ring 201 is fixedly connected to the top cover 203, and its bottom is fixed to the top of the inner tank 1. The outer wall of the connecting ring 201 is fixedly connected to a fixing column 202. The top of the top cover 203 is fixedly connected to the outer shell 204. The inner wall of the outer shell 204 is fixedly connected to a motor 205. The output end of the motor 205 is rotatably connected to the rotating shaft 206. The outer wall of the rotating shaft 206 is fixedly connected to the rotating component 207. The outer wall of the inner tank 1 is fixedly connected to the feeding component 208.

[0032] Specifically, the outer wall of the connecting ring 201 is fixedly connected to the fixing column 202 to ensure the stability of the overall structure. The top of the connecting ring 201 is tightly integrated with the top cover 203 to form an integrated structure. The top of the top cover 203 is fixedly connected to the outer shell 204. On the inner wall of the outer shell 204, the motor 205 is firmly fixed. The output end of the motor 205 is connected to the rotating shaft 206 through a rotating connection device. On the outer wall of the rotating shaft 206, the rotating component 207 is fixedly connected to it. On the outer wall of the inner tank 1, the feeding component 208 is also firmly fixed.

[0033] Please see the appendix Figure 3 - Appendix Figure 5 The grading mechanism 3 includes a processing tank 301, the bottom of which is fixedly connected to a top cover 203. A second rotating shaft 302 is fixedly connected to the outer wall of the processing tank 301, and a closing assembly 303 is rotatably connected to the outer wall of the second rotating shaft 302. A second motor 304 is fixedly connected to the outer wall of the processing tank 301, and a stirring blade 305 is rotatably connected to the output end of the second motor 304. A locking assembly 306 is fixedly connected to the right outer wall of the processing tank 301. An indicator 307 is fixedly connected to the top rear side of the first rotating shaft 206. The top of the top cover 203 is fixedly connected to... The bottom of the processing tank 301 is fixedly connected to the top cover 203. The outer wall of the processing tank 301 is fixedly connected to the second rotating shaft 302. The outer wall of the second rotating shaft 302 is rotatably connected to the closing component 303. The outer wall of the processing tank 301 is fixedly installed with the second motor 304. The output end of the second motor 304 is rotatably connected to the stirring blade 305. The right outer wall of the processing tank 301 is fixedly connected to the locking component 306. The top rear side of the first rotating shaft 206 is fixedly installed with an indicator 307. The top of the top cover 203 is fixedly connected to the docking component 308.

[0034] Specifically, the bottom of the processing tank 301 is connected to the top cover 203 via a fixing device. A rotating shaft 302 is precisely fixedly connected to the outer wall of the processing tank 301. The outer wall of the rotating shaft 302 is rotatably connected to the closing assembly 303, allowing the closing assembly 303 to rotate smoothly under the drive of the rotating shaft 302. A motor 304 is also fixedly connected to the outer wall of the processing tank 301. The output end of the motor 304 is connected to the stirring blade 305 via a rotating connection device, ensuring that the stirring blade 305 can perform stirring operations under the drive of the motor 304. On the right outer wall of the processing tank 301, a locking assembly 306 is fixedly connected. An indicator 307 is fixedly installed on the top rear side of the rotating shaft 206 to display the working status of the rotating shaft 206 in real time. The top of the top cover 203 is connected to a docking assembly 308 via a secure fixing device.

[0035] Please see the appendix Figure 2 - Appendix Figure 4 The heating mechanism 4 includes a heating tube 401, which is fixed to the outer wall of the inner tank 1. A drain pipe 402 is connected to the bottom of the heating tube 401. The feeding assembly 208 includes a buffer tank 2081, which is disposed on the outer wall of the inner tank 1. A guide pipe 2082 is fixedly connected to the top of the buffer tank 2081. The rotating assembly 207 includes a fan blade 2071, the inner wall of which is fixed to a rotating shaft 206. The bottom of the vessel is fixedly connected to the bottom shaft 2072, the bottom of the heating tube 401 is connected to the unblocking tube 402, the feeding assembly 208 includes a buffer tank 2081, the buffer tank 2081 is installed on the outer wall of the inner tank 1, the top of the buffer tank 2081 is fixedly connected to the guide tube 2082, the rotating assembly 207 includes a fan blade 2071, the inner wall of the fan blade 2071 is fastened to the rotating shaft 206, and the bottom of the rotating shaft 206 is fixedly connected to the bottom shaft 2072;

[0036] Specifically, the heating tube 401 is firmly installed on the outer wall of the inner tank 1. The bottom of the heating tube 401 is provided with a drain pipe 402 that is connected to it. The buffer tank 2081 is set on the outer wall of the inner tank 1 to facilitate the buffering and storage of materials. The top of the buffer tank 2081 is fixedly connected to a guide pipe 2082, which is used to guide the materials smoothly into the buffer tank 2081. The inner wall of the fan blade 2071 is firmly fixed to the rotating shaft 206 to ensure that the fan blade 2071 can rotate synchronously with the shaft. The bottom of the rotating shaft 206 is fixedly connected to the bottom shaft 2072 of the vessel.

[0037] Please see the appendix Figure 3 - Appendix Figure 4 The docking assembly 308 includes a flange 3081, the bottom of which is connected to the top cover 203, and a mating interface 3082 fixedly connected to the top of the flange 3081. The engaging assembly 306 includes a positioning plate 3061, which is fixed to the outer wall of the processing tank 301. An L-shaped plate 3062 is slidably connected to the outer wall of the positioning plate 3061. The closing assembly 303 includes a tank cover 3031, which is rotatably connected to a rotating shaft 302. The top of the 31 is fixedly connected to a handle 3032, the bottom of the flange 3081 is connected to the top cover 203, and the top of the flange is fixedly connected to the interface 3082. The engaging assembly 306 includes a positioning plate 3061, which is fixed to the outer wall of the processing tank 301. An L-shaped plate 3062 is slidably connected to the outer wall of the tank. The closing assembly 303 includes a tank cover 3031, which is rotatably connected to a rotating shaft 302. The top of the tank cover 3031 is fixedly connected to a handle 3032.

[0038] Specifically, the bottom of flange 3081 is firmly joined to top cover 203, and a mating interface 3082 is fixedly connected to the top of flange 3081. The engaging assembly 306 is composed of a positioning plate 3061, which is reliably fixedly installed on the outer wall of processing tank 301. An L-shaped plate 3062 is also slidably connected to the outer wall of positioning plate 3061, allowing the L-shaped plate 3062 to move flexibly when needed. The closing assembly 303 is a component used to close processing tank 301. It includes a tank cover 3031, which is mounted on rotating shaft 302 by a rotatable connection.

[0039] Working principle: When in use, start motor 205, and the output end of motor 205 drives the rotating shaft 206 to rotate, which in turn drives the fan blade 2071 to rotate. Heating tube 401 is provided on the outer wall of inner tank 1 to heat the raw materials in inner tank 1. Connecting ring 201 is connected to the top of inner tank 1 and fixed by fixing column 202 to prevent leakage. Buffer tank 2081 is connected to the outer wall of inner tank 1. Guide pipe 2082 is connected to the top of buffer tank 2081 so that fuel can be added through guide pipe 2082.

[0040] A processing tank 301 is located on the top right side of the top cover 203. When processing various raw materials, the L-shaped plate 3062 is slid upward along the positioning plate 3061. The handle 3032 is held and the tank cover 3031 is opened by rotating along the second rotating shaft 302. The raw materials are then placed in the processing tank 301. The second motor 304 is started, so that the output end of the second motor 304 drives the stirring blade 305 to rotate. After the initial processing is completed, the gate at the bottom of the processing tank 301 is opened to allow the material to enter the interior of the connecting ring 201 for a second reaction.

[0041] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A multi-enzyme cascade catalytic reactor, comprising an inner tank (1), characterized in that: A heating mechanism (4) is fixedly connected to the outer wall of the inner tank (1), and an outer tank (5) is fixedly connected to the outer wall of the heating mechanism (4). A reaction mechanism (2) is fixedly connected to the top of the inner tank (1), and a grading mechanism (3) is fixedly connected to the top of the reaction mechanism (2). The reaction mechanism (2) includes a connecting ring (201). The bottom of the connecting ring (201) is fixed to the top of the inner tank (1). A fixing column (202) is fixedly connected to the outer wall of the connecting ring (201). A top cover (203) is fixedly connected to the top of the connecting ring (201). A shell (204) is fixedly connected to the top of the top cover (203). A motor (205) is fixedly connected to the inner wall of the shell (204). A rotating shaft (206) is rotatably connected to the output end of the motor (205). A rotating component (207) is fixedly connected to the outer wall of the rotating shaft (206). A feeding component (208) is fixedly connected to the outer wall of the inner tank (1).

2. The multi-enzyme cascade catalytic reactor according to claim 1, characterized in that: The grading mechanism (3) includes a processing tank (301), the bottom of which is fixedly connected to a top cover (203). A rotating shaft (302) is fixedly connected to the outer wall of the processing tank (301). A closing assembly (303) is rotatably connected to the outer wall of the rotating shaft (302). A motor (304) is fixedly connected to the outer wall of the processing tank (301). A stirring blade (305) is rotatably connected to the output end of the motor (304). A locking assembly (306) is fixedly connected to the right outer wall of the processing tank (301). An indicator (307) is fixedly connected to the rear top of the rotating shaft (206). A docking assembly (308) is fixedly connected to the top of the top cover (203).

3. The multi-enzyme cascade catalytic reactor according to claim 1, characterized in that: The heating mechanism (4) includes a heating tube (401), which is fixed to the outer wall of the inner tank (1), and the bottom of the heating tube (401) is connected to a drain pipe (402).

4. The multi-enzyme cascade catalytic reactor according to claim 1, characterized in that: The rotating assembly (207) includes a fan blade (2071), the inner wall of which is fixed on a rotating shaft (206), and the bottom of the rotating shaft (206) is fixedly connected to a bottom shaft (2072).

5. The multi-enzyme cascade catalytic reactor according to claim 1, characterized in that: The feeding assembly (208) includes a buffer tank (2081), which is disposed on the outer wall of the inner tank (1), and a guide tube (2082) is fixedly connected to the top of the buffer tank (2081).

6. The multi-enzyme cascade catalytic reactor according to claim 2, characterized in that: The closing assembly (303) includes a can lid (3031), which is rotatably connected to a rotating shaft (302), and a handle (3032) is fixedly connected to the top of the can lid (3031).

7. The multi-enzyme cascade catalytic reactor according to claim 2, characterized in that: The locking assembly (306) includes a locking plate (3061), which is fixed to the outer wall of the processing tank (301), and an L-shaped plate (3062) is slidably connected to the outer wall of the locking plate (3061).

8. The multi-enzyme cascade catalytic reactor according to claim 2, characterized in that: The docking assembly (308) includes a flange (3081), the bottom of which is connected to the top cover (203), and the top of which is fixedly connected to a docking interface (3082).

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