A device for screening of bacteria enriched in coastal sediments

Abstract

This utility model discloses a screening device for enriched bacterial strains in coastal sediments, belonging to the field of bacterial strain screening technology. It includes: an enrichment unit comprising an enrichment container with a culture port at its top, a culture medium inlet at the upper end of one side wall, and an enrichment liquid outlet at the lower end of the other side; a dilution unit comprising a dilution container and a stirring mechanism within it, with an enrichment liquid inlet at one upper side of the dilution container, a water inlet at the upper end of the other side, and a dilution liquid outlet at the lower end; a conveying unit located between the enrichment liquid inlet and the enrichment liquid outlet; and a screening unit comprising at least one centrifuge, with a dispensing pipe between the centrifuge and the dilution liquid outlet, a dispensing pump mounted on the dispensing pipe, and at least one centrifuge input dropper connected to the outlet end of the dispensing pipe. This utility model fully automates operation, saving significant time and enabling efficient enrichment and separation of bacterial strains.

Description

Technical Field

[0001] This invention belongs to the field of strain screening technology, specifically relating to a strain screening device for enriched strains in coastal sediments. Background Technology

[0002] When enriching and screening strains from coastal sediments, enrichment culture containers are required. After enrichment culture, the strains are manually poured into dilution containers for dilution. The diluted solution is then added to the separation tube of a separator for separation and screening. The entire process cannot be automated, which wastes time and makes it impossible to achieve efficient enrichment and separation of strains, resulting in low screening efficiency. Utility Model Content

[0003] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0004] A screening device for strains enriched in coastal sediments includes:

[0005] An enrichment unit, comprising an enrichment container, wherein a culture port is provided at the top of the enrichment container, a culture medium injection port is provided at the upper end of one side wall of the enrichment container, and an enrichment medium discharge port is provided at the lower end of the other side.

[0006] The dilution unit includes a dilution container and a stirring mechanism disposed within the dilution container. The dilution container has an enrichment liquid inlet on one side of its upper end, a water inlet on the upper end of the other side of the dilution container, and a diluent outlet at its lower end. The stirring mechanism is used to stir and dilute the mixture of enrichment liquid and dilution water.

[0007] A conveying unit is disposed between the enriched liquid inlet and the enriched liquid outlet, and connects the enriched liquid inlet and the enriched liquid outlet to convey the enriched bacterial solution enriched in the enrichment container to the dilution container.

[0008] The screening unit includes at least one centrifuge, a dispensing pipe is provided between the at least one centrifuge and the outlet of the diluent, a dispensing pump is provided on the dispensing pipe, the outlet end of the dispensing pipe is connected to at least one centrifugal input dropper, the at least one centrifugal input dropper is correspondingly provided to at least one centrifuge, and a first solenoid valve is provided on the centrifugal input dropper.

[0009] Furthermore, the stirring mechanism includes a driving unit and a stirring shaft. The driving unit is located on the lower outer side of the dilution container. One end of the stirring shaft is fixedly connected to the driving end of the driving unit, and the other end extends into the dilution container. Multiple stirring plates are spaced apart on the stirring shaft located inside the dilution container.

[0010] Furthermore, the driving unit is a drive motor, which is fixedly installed on the lower outer side of the dilution container.

[0011] Furthermore, the conveying unit includes a conveying pipe connecting the enriched liquid inlet and the enriched liquid outlet, and a peristaltic pump mounted on the conveying pipe.

[0012] Furthermore, the enrichment liquid outlet is equipped with a second solenoid valve, and the diluent outlet is equipped with a third solenoid valve.

[0013] Furthermore, there are three centrifuges, and the outlet end of the dispensing pipe is connected to three centrifugal input droppers. The three centrifugal input droppers are arranged in a one-to-one correspondence with the three centrifuges. Each of the three centrifugal input droppers is equipped with a first solenoid valve.

[0014] Furthermore, a temperature sensor is provided on the inner wall of the enrichment container.

[0015] Beneficial effects:

[0016] This invention provides a screening device for enriched bacterial strains in coastal sediments. After enrichment culture, the strains are directly introduced into a dilution container for dilution, and then the diluted solution is added to the separation tube of the separator for separation and screening. The entire process is fully automated, saving a lot of time and enabling efficient enrichment and separation of bacterial strains with high screening efficiency. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0018] The components include: 1. Culture medium inlet; 2. Enrichment container; 3. Culture port; 4. Second solenoid valve; 5. Dilution container; 6. Stirring shaft; 7. Stirring plate; 8. Water inlet; 9. Dispensing pipe; 10. Centrifuge input dropper; 11. First solenoid valve; 12. First centrifuge; 13. Second centrifuge; 14. Third centrifuge; 15. Dispensing pump; 16. Third solenoid valve; 17. Drive motor; 18. Peristaltic pump; 19. Delivery pipe; and 20. Temperature sensor. Detailed Implementation

[0019] Example 1

[0020] refer to Figure 1 A screening device for strains enriched in coastal sediments, comprising:

[0021] The enrichment unit includes an enrichment container 2, a culture port 3 is provided at the top of the enrichment container 2, a culture medium injection port 1 is provided at the upper end of one side wall of the enrichment container 2, and an enrichment medium discharge port is provided at the lower end of the other side.

[0022] The dilution unit includes a dilution container 5 and a stirring mechanism disposed inside the dilution container 5. The dilution container 5 has an enrichment liquid inlet on one side of its upper end, a water inlet 8 on the upper end of the other side of the dilution container 5, and a dilution liquid outlet at its lower end. The stirring mechanism is used to stir and dilute the mixture of enrichment liquid and dilution water.

[0023] The conveying unit is located between the enrichment liquid inlet and the enrichment liquid outlet, and connects the enrichment liquid inlet and the enrichment liquid outlet to convey the enriched bacterial solution in the enrichment container 2 to the dilution container 5.

[0024] The screening unit includes at least one centrifuge. A dispensing pipe 9 is provided between the at least one centrifuge and the outlet of the diluent. A dispensing pump 15 is provided on the dispensing pipe 9. At least one centrifugal input dropper 10 is connected to the outlet end of the dispensing pipe 9. The at least one centrifugal input dropper 10 is correspondingly provided with at least one centrifuge. A first solenoid valve 11 is provided on the centrifugal input dropper 10.

[0025] In this embodiment, the stirring mechanism includes a driving part and a stirring shaft 6. The driving part is located on the lower outer side of the dilution container 5. One end of the stirring shaft 6 is fixedly connected to the driving end of the driving part, and the other end extends into the dilution container 5. Multiple stirring plates 7 are spaced apart on the stirring shaft 6 located inside the dilution container 5.

[0026] The drive unit is preferably a drive motor 17, which is fixedly installed on the lower outer side of the dilution container 5.

[0027] In this embodiment, the conveying unit includes a conveying pipe 19 connecting the enriched liquid inlet and the enriched liquid outlet, and a peristaltic pump 18 disposed on the conveying pipe 19.

[0028] In this embodiment, a second solenoid valve 4 is provided at the enrichment liquid outlet, and a third solenoid valve 16 is provided at the diluent outlet.

[0029] Specifically, there are three centrifuges, and the outlet end of the dispensing tube 9 is connected to three centrifuge input pipes 10, with each of the three centrifuge input pipes 10 corresponding to one of the three centrifuges. Each of the three centrifuge input pipes 10 is equipped with a first solenoid valve 11. The three centrifuges are respectively designated as a first centrifuge 12, a second centrifuge 13, and a third centrifuge 14. During centrifugation and screening, the first centrifuge 12, the second centrifuge 13, and the third centrifuge 14 are set to different operating speeds according to actual needs to obtain different strains.

[0030] In this embodiment, a temperature sensor 20 is provided on the inner wall of the enrichment container 2 to monitor the temperature of the culture medium inside the enrichment container 2; according to actual use needs, heating and cooling devices can be added inside the wall of the enrichment container 2 to adjust the temperature of the culture medium inside the enrichment container 2.

[0031] The working process of the coastal sediment enrichment strain screening device provided in this embodiment is as follows:

[0032] Culture medium is injected into enrichment container 2 through culture medium inlet 1, and coastal sediment strains are added through the culture inlet to enrich the coastal sediment strains. After enrichment, the solution is directly introduced into dilution container 5 for dilution. During dilution, water is injected into dilution container 5 through water inlet. A flow meter can be installed at the water inlet to monitor the amount of water injected into dilution container 5 in real time, depending on actual use needs. Then, the diluted solution is added into the separation tubes of three separators. The three separators are set with corresponding speeds through the control panel to separate and screen the strains. The entire process is fully automated, saving a lot of time and achieving high-efficiency enrichment and separation of strains with high screening efficiency.

[0033] The above description is merely a preferred embodiment of the present utility model and does not constitute any limitation on the technical scope of the present utility model. Therefore, any minor modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present utility model shall still fall within the scope of the technical solution of the present utility model.

Claims

1. A screening device for strains enriched in coastal sediments, characterized in that, include: An enrichment unit, comprising an enrichment container, wherein a culture port is provided at the top of the enrichment container, a culture medium injection port is provided at the upper end of one side wall of the enrichment container, and an enrichment medium discharge port is provided at the lower end of the other side. The dilution unit includes a dilution container and a stirring mechanism disposed within the dilution container. The dilution container has an enrichment liquid inlet on one side of its upper end, a water inlet on the upper end of the other side of the dilution container, and a diluent outlet at its lower end. The stirring mechanism is used to stir and dilute the mixture of enrichment liquid and dilution water. A conveying unit is disposed between the enriched liquid inlet and the enriched liquid outlet, and connects the enriched liquid inlet and the enriched liquid outlet to convey the enriched bacterial solution enriched in the enrichment container to the dilution container. The screening unit includes at least one centrifuge, a dispensing pipe is provided between the at least one centrifuge and the outlet of the diluent, a dispensing pump is provided on the dispensing pipe, the outlet end of the dispensing pipe is connected to at least one centrifugal input dropper, the at least one centrifugal input dropper is correspondingly provided to at least one centrifuge, and a first solenoid valve is provided on the centrifugal input dropper.

2. The screening device for enriched strains in coastal sediments according to claim 1, characterized in that, The stirring mechanism includes a drive unit and a stirring shaft. The drive unit is located on the lower outer side of the dilution container. One end of the stirring shaft is fixedly connected to the drive end of the drive unit, and the other end extends into the dilution container. Multiple stirring plates are spaced apart on the stirring shaft located inside the dilution container.

3. The screening device for enriched strains in coastal sediments according to claim 2, characterized in that, The driving unit is a drive motor, which is fixedly installed on the lower outer side of the dilution container.

4. The screening device for enriched strains in coastal sediments according to claim 1, characterized in that, The delivery unit includes a delivery pipe connecting the enriched liquid inlet and the enriched liquid outlet, and a peristaltic pump installed on the delivery pipe.

5. The screening device for enriched strains in coastal sediments according to claim 4, characterized in that, The enrichment liquid outlet is equipped with a second solenoid valve, and the diluent outlet is equipped with a third solenoid valve.

6. The screening device for enriched strains in coastal sediments according to claim 1, characterized in that, There are 3 centrifuges, and the outlet end of the dispensing pipe is connected to 3 centrifugal input droppers. The 3 centrifugal input droppers are set one-to-one with the 3 centrifuges. Each of the 3 centrifugal input droppers is equipped with a first solenoid valve.

7. The screening device for enriched strains in coastal sediments according to claim 1, characterized in that, A temperature sensor is installed on the inner wall of the enrichment container.

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