A uniaxial perfusion rotating cell culture device

Abstract

This utility model discloses a single-axis perfusion rotating cell culture device, including a fixed frame, a culture container assembly, a culture control device, a rotation drive assembly, and a peristaltic pump. An installation space is provided between the fixed frames. The culture control device and the rotation drive assembly are fixedly installed at the bottom of the installation space. An installation groove is fixedly opened on the upper surface of the fixed frame, and a locking assembly is provided above the installation groove. The culture container assembly is rotatably installed in the installation groove. The culture container assembly includes an outer culture container and a culture medium delivery device. One end of the culture medium delivery device has an inlet, which is fixedly connected to the peristaltic pump. The other end of the culture medium delivery device is fixedly connected to an outlet. A first transmission gear is fixedly installed on the outer ring surface of one end of the culture medium delivery device, and the first transmission gear is connected to the rotation drive assembly. This utility model supplies perfused culture medium to cells for absorption through rotation, and has a simple structure and is easy to use.

Description

Technical Field

[0001] This utility model relates to the field of automated machinery, and in particular to a single-axis perfusion rotating cell culture device. Background Technology

[0002] Cell culture is a key technology in biomedical research and is of great significance for drug development and disease treatment. Traditional static cell culture methods suffer from problems such as uneven cell growth and insufficient nutrient supply, which affect the effectiveness of cell culture.

[0003] In view of this, we propose a uniaxial perfusion rotating cell culture system to solve the existing problems. Utility Model Content

[0004] The main objective of this invention is to provide a uniaxial perfusion rotating cell culture device.

[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0006] A uniaxial perfusion rotary cell culture device includes a fixed frame, a culture container assembly, a culture control device, a rotary drive assembly, and a peristaltic pump. An installation space is provided between the fixed frames. The culture control device and the rotary drive assembly are fixedly installed at the bottom of the installation space. An installation groove is fixedly opened on the upper surface of the fixed frame. A locking assembly is provided above the installation groove. The culture container assembly is rotatably installed within the installation groove. The culture container assembly includes an outer culture container and a culture medium delivery device. The culture medium delivery device passes through the central axis of the outer culture container. One end of the culture medium delivery device has an inlet, which is fixedly connected to the peristaltic pump. The other end of the culture medium delivery device has an outlet. A first transmission gear is fixedly installed on the outer ring surface of one end of the culture medium delivery device, and the first transmission gear is drively connected to the rotary drive assembly.

[0007] Furthermore, the two ends of the culture delivery device are rotatably and sealingly connected to rotating seats, which are fitted into the mounting groove and locked in the mounting groove by a locking assembly.

[0008] Furthermore, the culture delivery device includes a culture medium delivery pipe and a permeation section, with the two ends of the permeation section respectively connected to the culture medium delivery pipe, which is connected to the inlet and outlet respectively.

[0009] Furthermore, the rotary drive assembly includes a rotary motor, a second transmission gear, and a third transmission gear. The second transmission gear is fixedly mounted on the output end of the rotary motor, and the third transmission gear is rotatably mounted on a fixed frame. The second transmission gear and the third transmission gear are connected by tooth meshing, and the third transmission gear is connected by tooth meshing with the first transmission gear.

[0010] Furthermore, the culture control device includes a control device body, which is electrically connected to the power source of the rotary drive assembly and the peristaltic pump, and the control device body is provided with an external port.

[0011] Furthermore, a culture chamber is provided between the interior of the external culture container and the culture delivery device, and the culture chamber has a cell seed inlet that communicates with the outside.

[0012] Furthermore, the locking assembly includes a locking pressure plate and locking screws. The mounting groove on the upper end face of the fixing frame is provided with threaded holes that match the locking screws on both sides. The locking pressure plate covers the mounting groove and is locked to the fixing frame by the locking screws.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0014] This invention supplies perfused culture medium to cells for absorption through rotation, and has a simple structure and is easy to assemble. Attached Figure Description

[0015] Figure 1 This is the front view of the present invention;

[0016] Figure 2 This is a top view of the present invention;

[0017] Figure 3 This is a perspective view of the present invention.

[0018] Figure Labels

[0019] 1. Fixing frame; 2. Culture container assembly; 3. Culture control device; 4. Rotation drive assembly; 5. Peristaltic pump; 6. Mounting slot; 7. Locking assembly; 8. External culture container; 10. Inlet; 11. Outlet; 12. First transmission gear; 13. Rotating seat; 14. Third transmission gear; 17. External port; 18. Cell seed inlet; 19. Locking plate; 20. Locking screw. Detailed Implementation

[0020] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, thereby providing a clearer and more definite definition of the scope of protection of the present invention.

[0021] See Figure 1-3As shown, a single-axis perfusion rotating cell culture device includes a fixed frame 1, a culture container assembly 2, a culture control device 3, a rotation drive assembly 4, and a peristaltic pump 5. An installation space is provided between the fixed frames 1. The culture control device 3 and the rotation drive assembly 4 are fixedly installed at the bottom of the installation space. An installation groove 6 is fixedly opened on the upper surface of the fixed frame 1. A locking assembly 7 is provided above the installation groove 6. The culture container assembly 2 is rotatably installed in the installation groove 6. The culture container assembly 2 includes an outer culture container 8 and a culture medium delivery device. The culture medium delivery device passes through the central axis of the outer culture container 8. One end of the culture medium delivery device has an inlet 10, which is fixedly connected to the peristaltic pump 5. The other end of the culture medium delivery device has an outlet 11. A first transmission gear 12 is fixedly provided on the outer ring surface of one end of the culture medium delivery device, and the first transmission gear 12 is connected to the rotation drive assembly 4.

[0022] A culture chamber is provided between the interior of the external culture container 8 and the culture delivery device, and the culture chamber is provided with a cell seeding inlet 18 that communicates with the outside.

[0023] The cultured cells are seeded into the culture chamber through the cell seeding inlet 18. Fresh culture medium is drawn in by the peristaltic pump 5 and sent into the culture delivery device through the liquid inlet 10 for cell absorption. The waste culture medium after metabolism flows out through the liquid outlet 11.

[0024] The two ends of the culture delivery device are rotatably and sealed with rotating seats 13. The rotating seats 13 are fitted into the mounting grooves 6 and locked in the mounting grooves 6 by the locking components 7.

[0025] The culture delivery device is rotatably connected to the inlet 10 and outlet 11 via rotating seats 13 at both ends, which facilitates the rotation of the culture delivery device.

[0026] The culture delivery device includes a culture medium delivery pipe and an osmosis section. The two ends of the osmosis section are respectively connected to the culture medium delivery pipe, which is connected to the inlet 10 and the outlet 11.

[0027] The permeation section includes a grid-like or perforated pipe, with a permeation diaphragm fixedly installed on the inner or outer wall of the pipe.

[0028] The rotary drive assembly 4 includes a rotary motor, a second transmission gear, and a third transmission gear 14. The second transmission gear is fixedly mounted on the output end of the rotary motor, and the third transmission gear 14 is rotatably mounted on the fixed frame 1. The second transmission gear and the third transmission gear 14 are connected by tooth meshing, and the third transmission gear 14 is connected by tooth meshing with the first transmission gear 12.

[0029] The culture control device 3 includes a control device body, which is electrically connected to the power source of the rotary drive assembly 4 and the peristaltic pump 5, and the control device body is provided with an external port 17.

[0030] The locking assembly 7 includes a locking pressure plate 19 and a locking screw 20. The mounting groove 6 on the upper end face of the fixing frame 1 has threaded holes on both sides that match the locking screw 20. The locking pressure plate 19 covers the mounting groove 6 and is locked to the fixing frame 1 by the locking screw 20.

[0031] The above description is only a preferred embodiment of the present utility model. The protection scope of the present utility model is not limited to the above embodiments. Any equivalent modifications or changes made by those skilled in the art based on the content disclosed in the present utility model should be included in the protection scope recorded in the claims.

Claims

1. A single-axis perfusion rotary cell culture device comprising a fixed frame (1), a culture container assembly (2), a culture control device (3), a rotary drive assembly (4) and a peristaltic pump (5), wherein a mounting space is provided between the fixed frame (1), characterized in that: The bottom of the installation space is fixedly installed with a culture control device (3) and a rotary drive assembly (4). The upper surface of the fixed frame (1) is fixedly provided with an installation groove (6). The mounting groove (6) is covered with a locking assembly (7). The culture container assembly (2) is rotatably installed in the mounting groove (6). The culture container assembly (2) includes an outer culture container (8) and a culture medium delivery device. The culture medium delivery device passes through the central axis of the outer culture container (8). One end of the culture medium delivery device is provided with an inlet (10). The inlet (10) is fixedly connected to a peristaltic pump (5). The other end of the culture medium delivery device is fixedly connected with an outlet (11). A first transmission gear (12) is fixedly provided on the outer ring surface of one end of the culture medium delivery device. The first transmission gear (12) is connected to the rotary drive assembly (4) for transmission.

2. A single-axial perfusion rotary cell culture device according to claim 1, wherein: The two ends of the culture medium delivery device are rotatably sealed with rotating seats (13), which are fitted into the mounting groove (6) and locked in the mounting groove (6) by the locking assembly (7).

3. The single-axial perfusion rotary cell culture device of claim 1, wherein: The culture medium delivery device includes a culture medium delivery pipe and an osmosis section. The two ends of the osmosis section are respectively connected to the culture medium delivery pipe, which is connected to the inlet (10) and the outlet (11).

4. The single-axial perfusion rotary cell culture device of claim 1, wherein: The rotary drive assembly (4) includes a rotary motor, a second transmission gear and a third transmission gear (14). The second transmission gear is fixedly mounted on the output end of the rotary motor. The third transmission gear (14) is rotatably mounted on the fixed frame (1). The second transmission gear and the third transmission gear (14) are connected by tooth meshing. The third transmission gear (14) is connected by tooth meshing with the first transmission gear (12).

5. The single-axial perfusion rotary cell culture device of claim 1, wherein: The culture control device (3) includes a control device body, which is electrically connected to the power source of the rotary drive assembly (4) and the peristaltic pump (5), and the control device body is provided with an external port (17).

6. The single-axial perfusion rotary cell culture device of claim 1, wherein: The external culture container (8) has a culture chamber between its interior and the culture delivery device, and the culture chamber has a cell seed inlet (18) that communicates with the outside.

7. The single-axial perfusion rotary cell culture device of claim 1, wherein: The locking assembly (7) includes a locking pressure plate (19) and a locking screw (20). The mounting groove (6) on the upper end face of the fixing frame (1) is provided with threaded holes that match the locking screw (20) on both sides. The locking pressure plate (19) covers the mounting groove (6) and is locked to the fixing frame (1) by the locking screw (20).

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