A sterile liquid sample collection-storage system with bacteria removal function

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By designing a sterile liquid sample collection and storage system with sterilization function, and using a 0.22-micron pore size filter and pipeline control device, the problem of microbial contamination in non-sterile environments was solved, achieving sterile sample collection and storage and extending shelf life.

CN224341283UActive Publication Date: 2026-06-09RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI

Patent Information

Authority / Receiving Office: CN · China
Patent Type: Utility models(China)
Current Assignee / Owner: RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Filing Date: 2025-07-23
Publication Date: 2026-06-09

AI Technical Summary

Technical Problem

Existing technologies are insufficient to effectively filter microorganisms in non-sterile environments, leading to the decomposition of sample components by microorganisms during the collection and storage of liquid samples, which affects the analytical results.

Method used

Design a sterile liquid sample collection and storage system with sterilization function. Employ a filter with a 0.22-micron pore size and a pipeline control device to achieve sterile sample collection and storage through the channel pipeline, preventing microbial contamination.

Benefits of technology

It effectively filters microorganisms, extends sample shelf life, and ensures the sterility of samples in non-sterile environments, making it suitable for various scenarios such as field collection and long-distance transportation.

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Abstract

This invention discloses a sterile liquid sample collection and storage system with sterilization function. The system includes a sample storage container and a filter; the sample storage container has one or more channel pipes; one end of each channel pipe is connected to the inside of the sample storage container, and the other end is free outside the sample storage container and connected to the filter. This system can effectively filter and remove microorganisms from liquid samples, preventing them from further decomposing substances in the sample, thereby effectively extending the shelf life of the liquid sample. It is particularly suitable for samples that lack sterile sampling conditions, on-site analysis conditions, or freezing / refrigerated transportation conditions, and can be used simultaneously in various scenarios and industries such as environmental water sample collection, fermentation broth sampling, and urine collection.

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Description

Technical Field

[0001] This utility model relates to a sterile liquid sample collection and storage system with sterilization function. Background Technology

[0002] Aseptic sampling of liquid samples in non-sterile environments presents a significant technical challenge across various industries. For instance, in field water quality analysis, current technologies struggle to achieve aseptic sample collection and storage. Microorganisms present in the sample continuously decompose and alter its components, leading to discrepancies between laboratory analysis results and actual findings. For example, microorganisms in environmental water samples continuously decompose and transform dissolved nitrogen and dissolved organic matter. Conventional methods like acidification or refrigeration cannot completely eliminate microbial interference and may also affect the analysis of other components.

[0003] Currently, existing water sampling and storage equipment is designed for collecting whole water samples and lacks sterilization capabilities. On-site sterilization using filters is susceptible to contamination by environmental microorganisms. Therefore, most existing water sampling and storage devices are unable to effectively filter microorganisms in the water and cannot adequately meet the requirements for water sample preservation time. Thus, developing a sterile liquid sample collection and storage system that can be used on-site, effectively filters microorganisms, and extends the preservation time of water samples is of significant practical importance. Utility Model Content

[0004] The purpose of this invention is to provide a sterile liquid sample collection and storage system with sterilization function. This system can effectively filter and remove microorganisms from liquid samples, preventing them from further decomposing the substances in the samples, thereby effectively extending the shelf life of liquid samples. It is especially suitable for samples that do not have sterile sampling conditions, on-site analysis conditions, or freezing / refrigeration transportation conditions. It can be used in various scenarios and industries such as environmental water sample collection, fermentation broth sampling, and urine collection.

[0005] This utility model provides a sterile liquid sample collection and storage system with sterilization function, including a sample storage container and a filter;

[0006] The sample storage container is provided with one or more channel pipes; one end of the channel pipe is connected to the inside of the sample storage container, and the other end is free outside the sample storage container and connected to the filter.

[0007] In the above-mentioned sterile liquid sample collection and storage system with sterilization function, a pipeline control device is installed on the channel pipeline to control the opening and closing of the pipeline.

[0008] In the above-mentioned sterile liquid sample collection and storage system with sterilization function, the pipeline control device includes a water-stop clamp or valve to have the function of sealing the pipeline to prevent the flow of gas and liquid.

[0009] In the above-mentioned sterile liquid sample collection and storage system with sterilization function, the filter is a filter with a pore size of 0.22 micrometers. The 0.22-micrometer filter can effectively intercept bacteria and fungi in the water, ensuring that the water sample entering the sample storage container is sterile.

[0010] The filter material has a 0.22-micron filtration function and is a material with the above-mentioned filtration function that can be obtained by those skilled in the art. Specifically, it can be selected from polyethersulfone, nylon, polyvinylidene fluoride, polytetrafluoroethylene or glass fiber, or devices containing metal or ceramic filter elements that also have a 0.22-micron filtration function.

[0011] In the above-mentioned sterile liquid sample collection and storage system with sterilization function, the sample storage container is selected from bag-shaped liquid storage containers and / or bottle-shaped liquid storage containers. The bag-shaped liquid storage container can be a bag-shaped container made of multilayer co-extruded film soft bag or any other material. The bottle-shaped liquid storage container can be made of at least one of high-density polyethylene, polypropylene, glass, PET, PVDF, or any other material that can be used to make bottle-shaped containers.

[0012] In the above-mentioned sterile liquid sample collection and storage system with sterilization function, the number of channel tubes can be 1 to 3.

[0013] In the aforementioned sterile liquid sample collection and storage system with sterilization function, when the sample storage container is equipped with one channel, the channel simultaneously serves as an injection channel, a sampling channel, and a gas balancing channel. Specifically, in use, the channel is used as an injection channel and then as a sampling channel. The channel can be flushed (during sampling, the channel and filter are cleaned by discarding the initial sample, i.e., by flushing the sampling channel with a portion of the sample in the storage bag). When the sample storage container is equipped with one channel, the sample storage container is preferably a bag-shaped storage container.

[0014] In the above-mentioned sterile liquid sample collection and storage system with sterilization function, when the sample storage container is provided with two channel pipes, it is configured as either 1) or 2) below: 1) one of the channel pipes is used as the sample inlet channel, and the other channel pipe is used as the sampling channel and gas balance channel; 2) one of the channel pipes is used as both the sample inlet channel and the sampling channel, and the other channel pipe is used as the gas balance channel.

[0015] In the above-mentioned sterile liquid sample collection and storage system with sterilization function, when the sample storage container is equipped with three channel pipelines, the three channel pipelines serve as the sample inlet channel, the sampling channel, and the gas balance channel, respectively.

[0016] In this invention, when the sample storage container is a bottle-shaped liquid storage container, one channel pipeline must serve as a gas balance channel.

[0017] When using this invention, the channel pipeline serves as a gas balance channel:

[0018] (1) Before the injection / sampling operation, turn on the pipeline control device to establish gas pressure balance;

[0019] (2) After the operation is completed, shut off the pipeline control device to block the path of microbial invasion;

[0020] (3) After the operation is completed, the filter at the end of the channel pipe blocks the path of microbial invasion;

[0021] (4) After the operation is completed, shut off the pipeline control device to prevent sample leakage.

[0022] In this invention, when the channel pipeline serves as a gas balance channel, an inert gas, such as nitrogen or argon, can be introduced into the gas balance channel to reduce the rate of spontaneous oxidation of chemical substances in the sampled sample.

[0023] In this invention, when the sample storage container is a bottle-shaped liquid storage container, and it is a rigid bottle-shaped liquid storage container, it is pre-vacuumed before use, and the sampling passage and the gas balance passage are closed. The filter of the sampling port is immersed in the sample to be taken, and automatic sampling is achieved by negative pressure filtration.

[0024] The aseptic sampling method using the sterile liquid sample collection-storage system with sterilization function described in this utility model includes the following steps:

[0025] (1) Pre-sterilization: The sterile liquid sample collection-storage system with sterilization function is initially sterilized by irradiation treatment;

[0026] (2) Dynamic filtration: The sample collected by the syringe is injected into the sample storage container through the filter, and microorganisms are trapped by the filter;

[0027] (3) Closed storage: The pipeline control device of all channel pipeline devices shall be shut down immediately after sampling;

[0028] (4) Aseptic sampling: After filtration through a 0.22-micron pore size filter in the sampling channel, sterile samples are obtained.

[0029] To prevent contamination during use, the sample storage container, the sample inlet / sampling channel / gas balance channel pipeline, and all connections are sealed to prevent external microorganisms from contaminating the samples within the system.

[0030] The sterile liquid sample collection and storage system with sterilization function described in this utility model is particularly suitable for the following scenarios in water quality monitoring, fermentation broth sampling, or medical testing:

[0031] (1) Collection of liquid samples in the field without refrigeration conditions;

[0032] (2) Collection of liquid samples in the field where there is no sterilization equipment or no aseptic operating conditions;

[0033] (3) Preservation of liquid samples transported over long distances;

[0034] (4) Sampling under other non-sterile conditions;

[0035] (5) Collection of other non-sterile liquid samples.

[0036] This utility model has the following beneficial effects:

[0037] 1. Sterilization function: The sample is filtered through a 0.22-micron pore size filter to remove microorganisms, and the 0.22-micron pore size filter prevents external microorganisms from re-contaminating the sample during storage and sampling, thus achieving the purpose of sample sterilization and aseptic storage.

[0038] 2. The pipeline control device is turned off after the setting operation is completed to block the path of microbial invasion.

[0039] 3. This utility model is applied to the fields of water quality monitoring, fermentation broth sampling or medical testing, and is particularly suitable for the following scenarios: collection of liquid samples in the field without refrigeration conditions; preservation of liquid samples transported over long distances; sampling in other non-sterile environments; collection of other non-sterile liquid samples. Attached Figure Description

[0040] Figure 1 This is a schematic diagram of the structure of the sterile liquid sample collection and storage system with sterilization function of this utility model, which has a single-channel pipeline.

[0041] Figure 2 This is a schematic diagram of the structure of the sterile liquid sample collection and storage system with sterilization function of this utility model, which has two channel pipelines (without gas balance channel).

[0042] Figure 3 This is a schematic diagram of the structure of the sterile liquid sample collection and storage system with sterilization function of this utility model, which has two channel pipelines (including a gas balance channel).

[0043] Figure 4 This is a schematic diagram of the structure of the sterile liquid sample collection and storage system with sterilization function of this utility model, which has three channels of pipeline.

[0044] The markings in the diagram are as follows:

[0045] 1, 2, 3 Filters; 4 Sample inlet channel; 5 Sampling channel; 6 Gas balance channel; 7 Water stop clamp; 8 Sample storage container. Detailed Implementation

[0046] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0047] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.

[0048] To facilitate understanding of this technical solution by those skilled in the art, the present invention will be further described below with reference to the accompanying drawings in the embodiments. The embodiments described are not intended to limit the present invention. All other embodiments obtained by those skilled in the art without inventive effort based on the present invention are within the protection scope of the present invention.

[0049] Example 1

[0050] like Figure 1 As shown, this embodiment provides a sterile liquid sample collection and storage system with sterilization function, including a sample storage container 8 and a filter 1 / 2;

[0051] The sample storage container 8 (which can be a liquid storage container made of multi-layer co-extruded film soft bag and / or high-density polyethylene or polypropylene, specifically a multi-layer co-extruded film soft bag) is equipped with a channel tube, which also serves as the sample inlet channel 4 and the sampling channel 5 (vacuumed before use to achieve automatic sampling function); one end of the channel tube is connected to the inside of the sample storage container 8, and the other end is free outside the sample storage container and connected to filter 1 / 2 (a filter with a pore size of 0.22 microns, specifically a filter made of polyethersulfone, nylon, polyvinylidene fluoride, polytetrafluoroethylene or glass fiber). Filter 1 serves as the filter for sample inlet, and filter 2 serves as the filter for sampling.

[0052] A pipeline control device (specifically, a stop clamp 7) is installed on the pipeline to control the opening and closing of the pipeline.

[0053] Example 2

[0054] like Figure 2 As shown, this embodiment provides a sterile liquid sample collection and storage system with sterilization function, including a sample storage container 8 and a filter 1 / 2;

[0055] The sample storage container 8 (specifically, a multi-layer co-extruded film soft bag) is equipped with two channels: one channel is used as the sample inlet channel 4, and the other channel is used as the sampling channel 5. Neither channel is used as a gas balance channel.

[0056] One end of each of the two channel tubes is connected to the sample storage container 8, and the other end is free outside the sample storage container 8 and connected to filter 1 / 2 (a filter with a pore size of 0.22 micrometers, which can be selected from polyethersulfone, nylon, polyvinylidene fluoride, polytetrafluoroethylene or glass fiber).

[0057] Each channel pipeline is equipped with a pipeline control device (specifically, a stop clamp 7) to control the opening and closing of the pipeline.

[0058] Example 3

[0059] like Figure 3 As shown, this embodiment provides a sterile liquid sample collection and storage system with sterilization function, including a sample storage container 8 and filters 1 / 2 / 3;

[0060] The sample storage container 8 (specifically, a liquid storage container made of multi-layer co-extruded film soft bag and / or high-density polyethylene or polypropylene) is equipped with two channel pipes. One channel pipe serves as both the sample inlet / sampling channel 4 / 5, and the other channel pipe serves as the gas balance channel 6.

[0061] One end of the channel tube is connected to the sample storage container 8, and the other end is free outside the sample storage container 8 and connected to the filter 1 / 2 / 3 (a filter with a pore size of 0.22 micrometers, which can be selected from polyethersulfone, nylon, polyvinylidene fluoride, polytetrafluoroethylene or glass fiber).

[0062] Each channel pipeline is equipped with a pipeline control device (specifically, a stop clamp 7) to control the opening and closing of the pipeline.

[0063] Example 4

[0064] like Figure 4 As shown, this embodiment provides a sterile liquid sample collection and storage system with sterilization function, including a sample storage container and a filter;

[0065] The sample storage container 8 (specifically, a liquid storage container made of multi-layer co-extruded film soft bag and / or high-density polyethylene or polypropylene) is equipped with 3 channel pipes, which serve as the sample inlet channel 4, the sampling channel 5, and the gas balance channel 6, respectively.

[0066] One end of each channel is connected to the sample storage container 8, and the other end is free outside the sample storage container 8 and connected to filters 1 / 2 / 3 (filters with a pore size of 0.22 micrometers, which can be selected from polyethersulfone, nylon, polyvinylidene fluoride, polytetrafluoroethylene or glass fiber).

[0067] A pipeline control device (specifically, a stop clamp 7) is installed on the pipeline to control the opening and closing of the pipeline.

[0068] When using this utility model, the channel pipeline serves as a gas balance channel: (1) Before the injection / sampling operation, the pipeline control device is turned on to establish gas pressure balance;

[0069] (2) After the operation is completed, shut off the pipeline control device to block the path of microbial invasion;

[0070] (3) After the operation is completed, the microbial invasion path is blocked by the filter at the end of the channel pipeline.

[0071] The aseptic sampling method using the sterile liquid sample collection and storage system with sterilization function of this invention includes the following steps:

[0072] (1) Pre-sterilization: The sterile liquid sample collection-storage system with sterilization function is initially sterilized by irradiation treatment;

[0073] (2) Dynamic filtration: The sample collected by the syringe is injected into the sample storage container through the filter, and microorganisms are trapped by the filter;

[0074] (3) Closed storage: The pipeline control devices of all channels and pipeline devices shall be shut down immediately after sampling;

[0075] (4) Aseptic sampling: After filtration through a 0.22-micron pore size filter in the sampling channel, sterile samples are obtained.

[0076] application:

[0077] The sample to be collected is river water (surface water). The specific collection steps are as follows:

[0078] The samples were filtered using rapid filter paper to remove large particulate impurities. The initial total bacterial count of the samples was 358 CFU / mL. The samples were then filtered using the device described in Example 4 of this invention and stored in sealed containers. Meanwhile, the control group was not treated and was stored in sterile containers in sealed containers. Three replicates were performed for each. After storing the experimental samples at 37°C for 7 days, the bacterial count was detected using agar medium under sterile conditions. The results showed that the bacterial counts in the samples collected and stored using the device described in Example 4 of this invention were all undetectable, while the control group counts were 256 CFU / mL, 302 CFU / mL, and 295 CFU / mL, respectively. This demonstrates that this invention achieves the purpose of sample sterilization and aseptic storage.

Claims

1. A sterile liquid sample collection and storage system with sterilization function, characterized in that, This includes sample storage containers and filters; The sample storage container is provided with one or more channel pipes; one end of the channel pipe is connected to the inside of the sample storage container, and the other end is free outside the sample storage container and connected to the filter.

2. The sterile liquid sample collection-storage system with sterilization function according to claim 1, characterized in that, A pipeline control device is installed on the channel pipeline to control the opening and closing of the pipeline.

3. The sterile liquid sample collection-storage system with sterilization function according to claim 2, characterized in that, The pipeline control device includes a water-stop clamp or a valve.

4. The sterile liquid sample collection-storage system with sterilization function according to claim 1 or 2, characterized in that, The filter is a filter with a pore size of 0.22 micrometers.

5. The sterile liquid sample collection-storage system with sterilization function according to claim 1 or 2, characterized in that, The sample storage container is selected from bag-shaped liquid storage containers and / or bottle-shaped liquid storage containers.

6. The sterile liquid sample collection-storage system with sterilization function according to claim 1 or 2, characterized in that, When the sample storage container is provided with one channel, the channel simultaneously serves as an injection channel, a sampling channel, and / or a gas balance channel.

7. The sterile liquid sample collection-storage system with sterilization function according to claim 1 or 2, characterized in that, When the sample storage container is provided with two channels, it is configured as either 1) or 2) below: 1) one of the channels is used as the sample inlet channel, and the other channel is used as the sampling channel and gas balance channel; 2) One of the channels serves as both the injection channel and the sampling channel, while the other channel serves as the gas balance channel.

8. The sterile liquid sample collection-storage system with sterilization function according to claim 1 or 2, characterized in that, When the sample storage container is equipped with three channels, the three channels serve as the sample inlet channel, the sampling channel, and the gas balance channel, respectively.

9. The sterile liquid sample collection-storage system with sterilization function according to claim 1 or 2, characterized in that, When the sample storage container is provided with more than 3 channels, one of the channels is used as a gas balance channel, and the remaining channels are used as sample inlet channels and / or sampling channels.