A sampling device for rhizosphere microorganisms of oryza sativa

Abstract

The utility model discloses a kind of Shan Lan rice root system microorganism sampling devices, including containing box and cutting knife, the bottom surface of containing box is provided with grid plate, the inner chamber central position of containing box is provided with the blank area of planting rice, containing box inner chamber bottom is provided with multiple square grooves, top plate is provided in square groove, lifting assembly that grid plate is provided in is driven top plate up and down movement, four side edges of square groove are all set up strip cutting hole, cutting knife is square tubular, grid plate divides multiple independent chambers, each chamber corresponds a square groove, so that sampling area is more explicit, improve sampling accuracy, lifting assembly can control top plate up and down movement, it is convenient to push out root system microorganism sample, it is convenient to collect, the cooperation of cutting knife and cutting hole and blade, it can accurately cut root system part soil, compared with traditional shovel, it greatly reduces the damage to surrounding root system microorganism.

Description

Technical Field

[0001] This utility model relates to the field of microbial sampling technology, and in particular to a root microbial sampling device for rice. Background Technology

[0002] As a unique rice variety, the study of its root microorganisms is of great significance for understanding its growth characteristics, ecological adaptability, and improving cultivation techniques. Precise root microbial sampling is fundamental to obtaining reliable data during the research process.

[0003] However, current methods for sampling root microorganisms in *Oryza sativa* have many drawbacks. Most existing operations rely on shovels, and this traditional tool has revealed serious limitations in practical applications.

[0004] In terms of sampling accuracy, the shovel has a large shovel surface and lacks precise positioning capabilities. The root system of *Rhizophora stylosa* is complex, and the structure and function of the root microbial community may vary significantly in different locations. When digging with a shovel, it is difficult to accurately collect root microorganisms from a specific area, resulting in samples that often contain a large amount of soil and microorganisms from unrelated surrounding areas.

[0005] From the perspective of damaging root microorganisms, the shovel, due to its large force and relatively rough digging method, is very likely to damage the surrounding root microorganisms when it penetrates the soil. Therefore, it is necessary to provide a root microorganism sampling device for *Rhizophora stylosa* to solve the above-mentioned technical problems. Utility Model Content

[0006] In view of the above situation and to overcome the defects of the existing technology, this utility model provides a root microbial sampling device for rice that can improve sampling accuracy and reduce damage to the surrounding root microorganisms.

[0007] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0008] A root microbial sampling device for *Rhizophora stylosa* includes a container and a cutting blade. The bottom surface of the container is provided with a grid plate, and the center of the inner cavity of the container is provided with a blank area for planting rice. The remaining positions on the bottom surface of the container's inner cavity, corresponding to the chambers of multiple grid plates, are provided with inwardly recessed square grooves. A top plate is movably installed in each square groove. A lifting component that drives the top plate to move up and down is provided in the chamber of the grid plate. Each of the four sides of the square groove has a strip-shaped cutting hole. The cutting blade is square tubular, and the bottom surface of the cutting blade is fixedly provided with blades that are compatible with the four cutting holes.

[0009] Preferably, the drive assembly includes a fixed plate, a cylinder, a telescopic rod, and an inflation unit. The fixed plate is fixedly installed in the cavity, and a cylinder is fixedly installed on the top surface of the fixed plate. A telescopic rod is movably inserted into the cylinder. One end of the cylinder passes through a square groove and is fixedly connected to the bottom surface of the top plate. A piston is installed at the other end. The inflation unit is used to inflate the cylinder with air.

[0010] Preferably, the inflation assembly includes a movable plate, an air bladder, and an elastic element. The movable plate is movably disposed in the cavity and located below the fixed plate via the elastic element. An air bladder is provided on the bottom surface of the movable plate. The air bladder is accordion-shaped and communicates with a cylinder. The fixed plate is also provided with an insertion hole adapted to the blade insertion.

[0011] Preferably, the elastic element is a spring, and multiple springs are provided, with an array of multiple elastic elements arranged around the airbag.

[0012] Preferably, the length of the blade is greater than the length of the cylinder.

[0013] Preferably, the top plate is also provided with an array of drainage holes.

[0014] Preferably, the container is also fixedly provided with a wire mesh, and the mesh openings of the wire mesh are arranged in a one-to-one correspondence with the square grooves.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) The design of the container of this utility model separates the planting area from the sampling area, which facilitates the cultivation and subsequent sampling of mountain rice. The grid plate divides into multiple independent chambers, each chamber corresponding to a square groove, making the sampling area clearer and improving the sampling accuracy. The lifting component can control the up and down movement of the top plate, which makes it easy to push out the root microbial samples for easy collection. The combination of the cutting knife, cutting hole and blade can accurately cut the soil in the root part, which greatly reduces the damage to the surrounding root microorganisms compared with the traditional shovel.

[0017] (2) The inflatable component of this utility model is designed such that when the cutting blade is pressed down, the blade is inserted into the insertion hole, pushing the movable plate to compress the elastic element, and the air in the airbag is forced into the cylinder to achieve automatic inflation. No additional external inflation equipment is required, and the operation is convenient. At the same time, the elastic element can reset the movable plate after cutting to prepare for the next inflation.

[0018] (3) The wire mesh of this utility model can guide the cutting blade when it cuts the sample from top to bottom, so that the cutting blade can pass smoothly through the strip cutting hole, cut the sample vertically, and pass smoothly through the insertion hole to push the movable plate downward, so that the sample is pushed upward, which further improves the stability of the device. Attached Figure Description

[0019] Figure 1 A schematic diagram of the overall structure of the root microbial sampling device for *Rhizophora stylosa* provided by this utility model;

[0020] Figure 2 for Figure 1 Front sectional view;

[0021] Figure 3 for Figure 1 Schematic diagram of the internal structure of the grid plate;

[0022] Figure 4 for Figure 1 Enlarged view of the center cutting blade;

[0023] Figure 5 for Figure 2 Enlarged view of the central roof panel and lifting components;

[0024] The corresponding names of the attached figures are: 1-containing box, 2-cutting knife, 3-grid plate, 4-square groove, 5-top plate, 6-cutting hole, 7-blade, 8-fixed plate, 9-cylinder, 10-telescopic rod, 11-piston, 12-moving plate, 13-airbag, 14-elastic element, 15-insertion hole, 16-drain hole, 17-wire mesh. Detailed Implementation

[0025] The present invention will be further described below with reference to the accompanying drawings and embodiments. The embodiments of the present invention include, but are not limited to, the following embodiments.

[0026] Example 1

[0027] like Figure 1-5As shown, this utility model provides a root microbial sampling device for *Rhizophora stylosa*, comprising a container 1 and a cutting blade 2. The bottom surface of the container 1 is provided with a grid plate 3. A blank area for planting rice is provided at the center of the inner cavity of the container 1. The remaining positions on the bottom surface of the inner cavity of the container 1, corresponding to the chambers of multiple grid plates 3, are provided with inwardly recessed square grooves 4. A top plate 5 is movably disposed within the square groove 4. A lifting assembly for driving the top plate 5 to move up and down is provided within the chambers of the grid plates 3. Strip-shaped cutting holes 6 are provided on the four sides of the square groove 4. The cutting blade 2 is square tubular. The bottom surface is fixed with blades 7 that are compatible with four cutting holes. The design of the holding box 1 separates the planting area from the sampling area, which facilitates the cultivation and subsequent sampling of the mountain rice. The grid plate 3 divides the area into multiple independent chambers, each corresponding to a square groove 4, making the sampling area clearer and improving the sampling accuracy. The lifting component can control the up and down movement of the top plate 5, which makes it easy to push out the root microbial samples for easy collection. The cutting blade 2, together with the cutting holes and blades 7, can accurately cut the soil in the root area, which greatly reduces the damage to the surrounding root microorganisms compared with traditional shovels.

[0028] Example 2

[0029] Please see Figure 2 The driving assembly includes a fixed plate 8, a cylinder 9, a telescopic rod 10, and an inflation unit. The fixed plate 8 is fixedly installed in the chamber, and the cylinder 9 is fixedly installed on the top surface of the fixed plate 8. The telescopic rod 10 is movably inserted into the cylinder 9. One end of the cylinder 9 passes through the square groove 4 and is fixedly connected to the bottom surface of the top plate 5. The other end is equipped with a piston 11. The inflation unit is used to inflate the cylinder 9 with air. By inflating the cylinder 9 with air through the inflation unit, the piston 11 is pushed, causing the telescopic rod 10 to drive the top plate 5 to rise, providing stable power for pushing out root microbial samples. The operation is simple and easy to control, and the position of the top plate 5 can be precisely adjusted, further improving the sampling accuracy.

[0030] Example 3

[0031] Please see Figure 2 and Figure 5 The inflation assembly includes a movable plate 12, an airbag 13, and an elastic element 14. The movable plate 12 is movably disposed in the cavity and located below the fixed plate 8 via the elastic element 14. The bottom surface of the movable plate 12 is provided with an airbag 13, which is accordion-shaped and communicates with the cylinder 9. The fixed plate 8 is also provided with a socket 15 that is compatible with the blade 7. When the cutting blade 2 is pressed down, the blade 7 is inserted into the socket 15, pushing the movable plate 12 to compress the elastic element 14. The air in the airbag 13 is forced into the cylinder 9, realizing automatic inflation without the need for additional external inflation equipment, making operation convenient. At the same time, the elastic element 14 can reset the movable plate 12 after cutting, preparing for the next inflation.

[0032] Example 4

[0033] Please see Figure 5 The elastic element 14 is a spring, and multiple elastic elements 14 are arranged in an array around the airbag 13. The multiple spring array arrangement can evenly support the movable plate 12, ensure the stability of the airbag 13 during compression and reset, make the inflation process more stable, and ensure the stability and accuracy of the rising action of the top plate 5.

[0034] Example 5

[0035] Please see Figure 4 The length of the blade 7 is greater than the length of the cylinder 9, ensuring that the blade 7 has sufficient length to penetrate into the soil for cutting after being inserted into the cutting hole. At the same time, it can be inserted into the insertion hole 15 and push the movable plate 12 downward to compress the air bag 13, thereby inflating the cylinder 9 and ensuring that the sample can be successfully ejected.

[0036] Example 6

[0037] Please see Figure 5 The top plate 5 is also provided with an array of drainage holes 16, which can drain water from the soil and achieve the function of filtering water without the need for subsequent filtration through a sieve, thus further facilitating sample collection.

[0038] Example 7

[0039] Please see Figure 1 The container 1 is also fixedly provided with a wire mesh 17. The mesh openings of the wire mesh 17 correspond one-to-one with the square grooves 4. The wire mesh 17 can guide the cutting blade 2 when it cuts the sample from top to bottom, so that the cutting blade 2 can pass smoothly through the strip cutting hole 6, cut the sample vertically, and pass smoothly through the insertion hole 15 to push the movable plate 12 downward, so that the sample is pushed upward, which further improves the stability of the device.

[0040] Working principle:

[0041] When using the root microbial sampling device for *Rhizophora stylosa*, first plant *Rhizophora stylosa* in the blank area of ​​the container 1. When sampling is required, align the cutting blade 2 with the wire mesh 17 above the square groove 4 and press down. Insert the blade 7 into the strip cutting hole 6 and the insertion hole 15 on the fixing plate 8. Push the movable plate 12 to compress the elastic element 14. Air in the airbag 13 is filled into the cylinder 9. The telescopic rod 10 extends and drives the top plate 5 to rise, lifting the root microbial sample along with the surrounding soil. Because the blade 7 is inserted into the soil for precise cutting, damage to the surrounding root microorganisms is reduced. During the rise of the top plate 5, the water in the soil is discharged through the drainage hole 16. After sampling is completed, release the cutting blade 2. The elastic element 14 resets the movable plate 12, and the airbag 13 is re-inflated. The entire device achieves high-precision and low-damage root microbial sampling of *Rhizophora stylosa* through reasonable structural design.

[0042] The above embodiments are merely one of the preferred embodiments of this utility model and should not be used to limit the scope of protection of this utility model. Any modifications or refinements made to the main design concept and spirit of this utility model that are not of substantial significance, but solve the same technical problem as this utility model, should be included within the scope of protection of this utility model.

Claims

1. A root microbial sampling device for *Oryza sativa*, characterized in that, The container includes a holding box (1) and a cutting knife (2). The bottom surface of the holding box (1) is provided with a grid plate (3). The center of the inner cavity of the holding box (1) is provided with a blank area for planting rice. The remaining positions of the bottom surface of the inner cavity of the holding box (1) are provided with inwardly recessed square grooves (4) corresponding to the cavities of multiple grid plates (3). A top plate (5) is movably provided in the square groove (4). A lifting component that drives the top plate (5) to move up and down is provided in the cavity of the grid plate (3). The four sides of the square groove (4) are provided with strip-shaped cutting holes (6). The cutting knife (2) is in the shape of a square tube. The bottom surface of the cutting knife (2) is fixedly provided with blades (7) that are compatible with the four cutting holes.

2. The root microbial sampling device for *Oryza sativa* according to claim 1, characterized in that, It also includes a drive assembly, which includes a fixed plate (8), a cylinder (9), a telescopic rod (10) and an inflation unit. The fixed plate (8) is fixedly installed in the cavity, and the cylinder (9) is fixedly installed on the top surface of the fixed plate (8). The telescopic rod (10) is movably inserted into the cylinder (9). One end of the cylinder (9) passes through the square groove (4) and is fixedly connected to the bottom surface of the top plate (5). The other end is equipped with a piston (11). The inflation unit is used to inflate the cylinder (9) with air.

3. The root microbial sampling device for *Oryza sativa* according to claim 2, characterized in that, It also includes an inflation assembly, which includes a movable plate (12), an airbag (13) and an elastic element (14). The movable plate (12) is movably disposed in the cavity and located below the fixed plate (8) through the elastic element (14). An airbag (13) is provided on the bottom surface of the movable plate (12). The airbag (13) is accordion-shaped and communicates with the cylinder (9). The fixed plate (8) is also provided with an insertion hole (15) that is compatible with the blade (7).

4. The root microbial sampling device for *Oryza sativa* according to claim 3, characterized in that, The elastic element (14) is a spring, and multiple elastic elements (14) are arranged in an array around the airbag (13).

5. The root microbial sampling device for *Oryza sativa* according to claim 4, characterized in that, The length of the blade (7) is greater than the length of the cylinder (9).

6. The root microbial sampling device for *Oryza sativa* according to claim 1, characterized in that, The top plate (5) is also provided with an array of drainage holes (16).

7. The root microbial sampling device for *Oryza sativa* according to claim 1, characterized in that, The container (1) is also fixedly provided with a wire mesh (17), and the mesh holes of the wire mesh (17) are set one-to-one with the square grooves (4).

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