A sample collection and detection integrated device

Abstract

The utility model discloses a sample collection detects integrated device belongs to medical examination testing instrument, molecular diagnostic detection instrument field, and its technical key points lie in, include: pipe cover subassembly, reagent pipe subassembly, puncture base, reaction tube, reagent pipe subassembly includes: reagent pipe body, piston rod body, ration cavity, reflux cavity, be provided with ration cavity and reflux cavity in the bottom of reagent pipe body, and the bottom of ration cavity and reflux cavity all are provided with the opening and all adopt the sealing film sealing, the piston rod body is placed in reagent pipe body, the top of puncture base is provided with first puncture pipe, second puncture pipe, the position of pressure column body and piston rod body corresponds, and the downward press pressure column body can drive piston rod body to enter into ration cavity. The utility model proposes the technical scheme, can prevent the solution in the test cavity to enter into the reaction tube again, thereby avoided the possibility of test cavity solution to enter the interference when the reaction tube carries out amplification reaction.

Description

Technical Field

[0001] This utility model relates to the technical field of medical examination and testing instruments and molecular diagnostic testing instruments, specifically to an integrated sample collection and testing device. Background Technology

[0002] Point-of-care testing (POCT) devices are a common consumable in molecular diagnostic instruments. A typical POCT device, such as the one disclosed in CN218989258U for nucleic acid detection, includes a first tubing assembly for storing a buffer solution and a second tubing assembly for storing a reaction solution. The second tubing assembly is connected to the first tubing assembly via a puncture tube.

[0003] The above-mentioned POCT device has the following disadvantages:

[0004] (1) The sample cannot be quantitatively transferred from the buffer solution to the solution in the reaction tube.

[0005] (2) During the reaction, the chamber containing the buffer solution and the chamber containing the reaction solution cannot be isolated from each other. Utility Model Content

[0006] The purpose of this invention is to address the shortcomings of the existing technology by providing an integrated sample collection and detection device.

[0007] The technical solution of this utility model is as follows:

[0008] An integrated sample collection and testing device includes: a tube cap assembly, a reagent tube assembly, a puncture base, and a reaction tube;

[0009] The pipe cap assembly includes: a pipe cap body, a placement chamber, a pressure column body, and a first sealing membrane; the placement chamber is provided in the middle of the pipe cap body; an opening is provided at the bottom of the placement chamber and the opening is sealed by the first sealing membrane; part of the pressure column body is placed into the placement chamber and part of it protrudes from the top of the placement chamber.

[0010] The reagent tube assembly includes: a reagent tube body, a piston rod, a metering chamber, and a reflux chamber; the metering chamber and the reflux chamber are provided at the bottom of the reagent tube body, and both the bottom of the metering chamber and the reflux chamber are provided with openings and are sealed with sealing membranes; the piston rod is placed in the reagent tube body;

[0011] The puncture base is provided with a first puncture tube and a second puncture tube at its top; the first puncture tube and the second puncture tube are respectively used to connect to the bottom opening of the quantitative chamber and the bottom opening of the reflux chamber; the bottom of the puncture base is provided with a first opening and a second opening that communicate with the first puncture tube and the second puncture tube.

[0012] The reaction tube is used to store the reaction solution and can be fixed to the bottom of the puncture base. It is connected to the first opening and the second opening.

[0013] The cap body is connected to / separated from the top of the reagent tube body. When the two are connected, the position of the pressure column body and the piston rod body are corresponding. Pressing down on the pressure column body can drive the piston rod body into the quantitative chamber.

[0014] An integrated sample collection and testing device includes: a tube cap assembly, a reagent tube assembly, a puncture base, a reaction tube, and a balloon;

[0015] The pipe cap assembly includes: a pipe cap body, a placement chamber, a pressure column body, and a first sealing membrane; the placement chamber is provided in the middle of the pipe cap body; an opening is provided at the bottom of the placement chamber and the opening is sealed by the first sealing membrane; part of the pressure column body is placed into the placement chamber and part of it protrudes from the top of the placement chamber.

[0016] The reagent tube assembly includes: a reagent tube body, a piston rod, and a metering chamber; a metering chamber is provided at the bottom of the reagent tube body, and the bottom of the metering chamber is provided with an opening and sealed with a sealing film; the piston rod is placed in the reagent tube body;

[0017] The puncture base is equipped with a first puncture tube and a balloon at its top; the first puncture tube is used to connect to the bottom opening of the metering chamber.

[0018] The reaction tube is used to store the reaction solution and can be fixed to the bottom of the puncture base. It is connected to the first puncture tube and the balloon.

[0019] The cap body is connected to / separated from the top of the reagent tube body. When the two are connected, the position of the pressure column body and the piston rod body are corresponding. Pressing down on the pressure column body can drive the piston rod body into the quantitative chamber.

[0020] Furthermore, the reaction tube includes an inlet pipe and an outlet pipe, which are respectively connected to a first opening and a second opening.

[0021] Furthermore, the piston rod body is adapted to the cross-sectional shape of the metering cavity.

[0022] Furthermore, a piston moving chamber is provided at the top of the metering chamber for the piston rod to move; in the initial state, a portion of the piston rod is inserted into the piston moving chamber.

[0023] Furthermore, a partition member is provided in the middle of the reagent tube body, and an opening is provided in the middle of the partition member, and the opening is connected to the upper opening of the quantitative cavity.

[0024] The upper part of the partition member of the reagent tube body forms a reagent chamber for containing buffer solution; the lower part of the piston moving chamber has a hole, so that the reagent chamber is connected to the quantitative chamber.

[0025] Furthermore, the reflux chamber is connected to the reagent chamber.

[0026] Furthermore, the upper surface of the partition component is inclined with a lower center and a higher circumference.

[0027] Furthermore, the reaction tube is a duckbill tube or a straight reaction tube.

[0028] The beneficial effects of this application are as follows:

[0029] First, the reagent chamber and the detection chamber of this application are independent of each other. After assembly, pressing the main body of the pressure column drives the piston rod to move downward, and the piston rod squeezes the solution in the quantitative chamber into the detection chamber (i.e., the reaction tube). Furthermore, the piston rod enters the quantitative chamber (the cross-sectional shape of the piston rod and the quantitative chamber are adapted to each other, which ensures that the solution above the piston rod cannot enter the bottom of the piston rod. Thus, after the amplification process in the reaction tube, the buffer solution will not enter the reaction tube). This prevents the solution in the test chamber from entering the reaction tube again, thereby avoiding the possibility of interference from the solution in the test chamber entering the reaction tube during the amplification reaction.

[0030] Second, this application designs a quantitative chamber and a reflux chamber / balloon. The top of the puncture base is provided with a first puncture tube and a second puncture tube. The reaction tube is connected to the first opening and the second opening provided at the bottom of the puncture base. After the piston rod pushes the solution in the quantitative chamber into the reaction tube, the excess gas / liquid in the reaction tube can enter the reflux chamber / balloon through the second opening. Attached Figure Description

[0031] The present invention will be further described in detail below with reference to the embodiments shown in the accompanying drawings, but this does not constitute any limitation on the present invention.

[0032] Figure 1 This is an elevation view of an integrated sample collection and detection device according to Embodiment 1 (the pressure column body and piston rod are both in their initial positions).

[0033] Figure 2 This is an elevation view of an integrated sample collection and detection device according to Embodiment 1 (the pressure column body and piston rod are both in the final position).

[0034] Figure 3 This is a three-dimensional design schematic diagram of the pipe cap assembly in Embodiment 1.

[0035] Figure 4 This is a cross-sectional view of the tube cap assembly and reagent tube assembly of Example 1 (the pressure column body and piston rod are both in the initial position).

[0036] Figure 5 This is a three-dimensional design schematic diagram of the puncture base in Embodiment 1.

[0037] Figure 6 This is a three-dimensional design schematic diagram of the puncture base of Embodiment 1 from another perspective.

[0038] Figure 7 This is a three-dimensional design schematic diagram of the connection between the puncture base and the duckbill tube in Embodiment 1.

[0039] Figure 8 This is an elevation view of the duckbill tube in Example 1.

[0040] Figure 9 This is a schematic diagram of the limiting block of the piston rod in Embodiment 1.

[0041] Figure 10 This is a cross-sectional view of the reaction tube in Example 2, which uses a straight-type reaction tube.

[0042] Figure 11 This is a three-dimensional structural diagram of the puncture base and balloon in Example 3.

[0043] Figure 12 This is an elevation view of an integrated sample collection and detection device according to Embodiment 3 (the pressure column body and piston rod are both in the initial position).

[0044] Figures 1-10 The annotations in the accompanying drawings are explained as follows:

[0045] 1000 integrated sample collection and testing device;

[0046] Pipe cap assembly 100, pipe cap body 101, placement chamber 102, pressure column body 103, first sealing membrane 104;

[0047] The reagent tube assembly 200, the reagent tube body 201, the piston rod 202, the limiting block 2021, the metering chamber 203, the second sealing membrane 204, the piston moving chamber 205, the partition member 206, and the reflux chamber 207.

[0048] Puncture base 300, first puncture tube 301, second puncture tube 302;

[0049] Duckbill tube 400;

[0050] 500 linear circular reaction tube;

[0051] 600 balloons. Detailed Implementation

[0052] <Example 1>

[0053] Figure 1 A three-dimensional structural diagram of a sample collection and detection integrated device 1000 is shown (the pressure column body is in the initial state). Figure 2 A three-dimensional structural diagram of a sample acquisition and detection integrated device 1000 is shown (the pressure column body is in a depressed state). From Figure 1 and Figure 2 It can be seen that a sample collection and detection integrated device 1000 includes:

[0054] (1) Pipe cap assembly 100. Figure 3 The diagram illustrates the three-dimensional structure of the pipe cap assembly 100. The pipe cap assembly 100 includes: a pipe cap body 101, a placement chamber 102, a pressure column body 103, and a first sealing membrane 104; the placement chamber 102 is disposed in the middle of the pipe cap body 101; the first sealing membrane 104 is disposed at the bottom of the placement chamber 102; the lower part of the pressure column body 103 is disposed in the placement chamber 102, and the upper part protrudes from the placement chamber 102.

[0055] (2) Reagent tube assembly 200. Figure 4 The diagram illustrates the three-dimensional structure of the reagent tube assembly 200. The reagent tube assembly 200 includes: a reagent tube body 201, a piston rod 202, a metering chamber 203 (with openings at both the top and bottom), and a reflux chamber 207 (with an opening only at the bottom).

[0056] The lower part of the reagent tube body 201 is provided with a quantitative cavity 203, and the bottom of the quantitative cavity 203 is provided with an opening; the top of the quantitative cavity 203 is provided with a piston moving cavity 205 for the piston rod 202 to move; a part of the piston rod 202 is inserted into the piston moving cavity 205.

[0057] More specifically, a partition member 206 is provided in the middle of the reagent tube body 201, and an opening is provided in the middle of the partition member 206, and the opening is connected to the upper opening of the quantitative cavity 203.

[0058] The upper part of the partition member 206 of the reagent tube body 201 forms a cavity for containing reagents (i.e., reagent cavity); the lower part of the piston moving cavity 205 has a hole, so that the cavity for containing reagents is connected to the metering cavity 203.

[0059] The lower part of the reagent tube body 201 is provided with a reflux chamber 207, and the bottom of the reflux chamber 207 is provided with an opening. The opening at the bottom of the quantitative chamber 203 and the opening at the bottom of the reflux chamber 207 are sealed by a second sealing membrane.

[0060] More specifically, such as Figure 9As shown, a limiting block 2021 is provided on the outer side of the piston rod 202. When the piston rod 202 moves downward, the limiting block 2021 contacts the top of the piston moving cavity 205, thereby achieving the limiting function.

[0061] More specifically, the reflux chamber is connected to the reagent chamber. Initially, the reflux chamber contains no buffer solution. During the process of the solution in the metering chamber 203 entering the duckbill tube 400, when the reflux chamber and reagent chamber are connected, their air pressures remain consistent. This facilitates the descent of the piston rod 202. Simultaneously, it prevents the piston rod 202 from rebounding when released after descent.

[0062] (3) Puncture base 300. Figure 5 and Figure 6 The structure of the puncture base 300 is illustrated from different angles. The top of the puncture base 300 is provided with a first puncture tube 301 and a second puncture tube 302; the bottom of the puncture base 300 is provided with a first opening and a second opening communicating with the first puncture tube 301 and the second puncture tube 302; the shape of the puncture base 300 is adapted to the bottom of the reagent tube body 201.

[0063] The first puncture tube 301 and the second puncture tube 302 correspond to the bottom opening of the metering chamber 203 and the bottom opening of the reflux chamber 207, respectively.

[0064] (4) Duckbill tube 400. Figure 7 and Figure 8 The structural design of the duckbill tube 400 is illustrated. The duckbill tube 400 can adopt a structure similar to that of CN116622500B, which includes: an inlet pipe and an outlet pipe; the inlet pipe is connected to the first puncture tube, and the outlet pipe is connected to the second puncture tube, that is, the inlet pipe and the outlet pipe are respectively connected to the first opening and the second opening.

[0065] The working method of the integrated testing consumables and testing device of this application is as follows:

[0066] S1, Place the sample into the reagent tube body 201;

[0067] S2, assemble the tube cap body 101 of the tube cap assembly 100 with the reagent tube body 201, and leave it stationary for a period of time to allow the sample to fully react with the reagent in the reagent tube body 201.

[0068] S3, assemble the puncture base 300 and the duckbill tube 400 into one piece, and then connect the puncture base 300 to the bottom of the reagent tube body 201. The first puncture tube 301 and the second puncture tube 302 puncture the second sealing membrane 204, so that the reagent chamber is connected to the detection chamber and the detection chamber is connected to the reflux chamber.

[0069] S4, Press the pressure column body 103: After the pressure column body 103 penetrates the first sealing membrane 104, it contacts the piston rod body 202. Then, the pressure column body 103 and the piston rod body 202 move downward together. The piston rod body 202 pushes the reaction liquid in the metering chamber 203 from the first puncture tube into the duckbill tube 400. The excess gas / liquid in the duckbill tube 400 enters the reflux chamber from the second puncture tube.

[0070] S5, at this point, the reagent in the duckbill tube 400 is heated and amplified, and then detected by light to obtain the final detection result.

[0071] <Example 2>

[0072] The structure of Example 2 is the same as that of Example 1, except for the reaction tube. Figure 10 A design scheme using a straight-line reaction tube is presented. For example... Figure 10 As shown, the reaction tube is a straight circular reaction tube 500, and the bottoms of the first puncture tube and the second puncture tube are connected to the reaction tube.

[0073] The piston rod 202 draws the reaction solution from the metering chamber 203 into the linear circular reaction tube 500 through the first puncture tube. The air / solution in the linear circular reaction tube 500 enters the reflux chamber through the second puncture tube.

[0074] That is, the lower openings of the first and second puncture tubes should be connected to the top openings of the reaction tube.

[0075] <Example 3>

[0076] like Figure 11 and Figure 12 As shown, the difference between Embodiment 3 and Embodiment 1 is only that the reflux chamber 207 of the reagent tube assembly 200 is omitted. Instead, a separate balloon 600 is used.

[0077] Correspondingly, the top of the puncture base is provided with: a first puncture tube 301 and a balloon 600; the first puncture tube 301 is used to connect to the bottom opening of the metering chamber;

[0078] The bottom of the puncture base 300 is provided with a first opening and a second opening that communicate with the first puncture tube 301 and the balloon 600;

[0079] The inlet and outlet pipes of the duckbill tube 400 are connected to the first opening and the second opening, respectively.

[0080] The piston rod 202 draws the reaction liquid from the metering chamber 203 into the duckbill tube through the first puncture tube. Excess gas / liquid in the duckbill tube enters the balloon through the second opening. The balloon 600 achieves pressure balance between the duckbill tube and the reagent tube body through self-inflation.

[0081] It should be noted that the duckbill tube 400 in Example 3 can also be replaced with the straight circular reaction tube 500 used in Example 2.

[0082] The above-described embodiments are preferred embodiments of the present utility model and are only used to facilitate the illustration of the present utility model. They are not intended to limit the present utility model in any way. Any person skilled in the art who makes partial modifications or alterations to the technical content disclosed in the present utility model without departing from the scope of the technical features of the present utility model shall still fall within the scope of the technical features of the present utility model.

Claims

1. A sample collection and detection integrated device, characterized in that, include: Tube cap assembly, reagent tube assembly, puncture base, reaction tube; The pipe cap assembly includes: a pipe cap body, a placement chamber, a pressure column body, and a first sealing membrane; the placement chamber is provided in the middle of the pipe cap body; an opening is provided at the bottom of the placement chamber and the opening is sealed by the first sealing membrane; part of the pressure column body is placed into the placement chamber and part of it protrudes from the top of the placement chamber. The reagent tube assembly includes: a reagent tube body, a piston rod, a metering chamber, and a reflux chamber; the metering chamber and the reflux chamber are provided at the bottom of the reagent tube body, and both the bottom of the metering chamber and the reflux chamber are provided with openings and are sealed with sealing membranes; the piston rod is placed in the reagent tube body; The puncture base is provided with a first puncture tube and a second puncture tube at its top; the first puncture tube and the second puncture tube are respectively used to connect to the bottom opening of the quantitative chamber and the bottom opening of the reflux chamber; the bottom of the puncture base is provided with a first opening and a second opening that communicate with the first puncture tube and the second puncture tube. The reaction tube is used to store the reaction solution and can be fixed to the bottom of the puncture base. It is connected to the first opening and the second opening of the puncture base. The cap body is connected to / separated from the top of the reagent tube body. When the two are connected, the position of the pressure column body and the piston rod body are corresponding. Pressing down on the pressure column body can drive the piston rod body into the quantitative chamber.

2. A sample collection and detection integrated device, characterized in that, include: Tube cap assembly, reagent tube assembly, puncture base, reaction tube, balloon; The pipe cap assembly includes: a pipe cap body, a placement chamber, a pressure column body, and a first sealing membrane; the placement chamber is provided in the middle of the pipe cap body; an opening is provided at the bottom of the placement chamber and the opening is sealed by the first sealing membrane; part of the pressure column body is placed into the placement chamber and part of it protrudes from the top of the placement chamber. The reagent tube assembly includes: a reagent tube body, a piston rod, and a metering chamber; a metering chamber is provided at the bottom of the reagent tube body, and the bottom of the metering chamber is provided with an opening and sealed with a sealing film; the piston rod is placed in the reagent tube body; The puncture base is equipped with a first puncture tube and a balloon at its top; the first puncture tube is used to connect to the bottom opening of the metering chamber. The reaction tube is used to store the reaction solution and can be fixed to the bottom of the puncture base. It is connected to the first puncture tube and the balloon. The cap body is connected to / separated from the top of the reagent tube body. When the two are connected, the position of the pressure column body and the piston rod body are corresponding. Pressing down on the pressure column body can drive the piston rod body into the quantitative chamber.

3. The sample collection and detection integrated device according to claim 1 or 2, characterized in that, The reaction tube includes: The liquid inlet pipe and the liquid outlet pipe are respectively connected to the first opening and the second opening.

4. The integrated sample collection and detection device according to claim 1 or 2, characterized in that, The piston rod is adapted to the cross-sectional shape of the metering cavity.

5. The integrated sample collection and detection device according to claim 1 or 2, characterized in that, The top of the metering chamber is provided with a piston moving chamber for the piston rod to move; in the initial state, a part of the piston rod is inserted into the piston moving chamber.

6. The integrated sample collection and detection device according to claim 5, characterized in that, A partition member is provided in the middle of the reagent tube body, and an opening is provided in the middle of the partition member and the opening is connected to the upper opening of the quantitative cavity. The upper part of the partition member of the reagent tube body forms a reagent chamber for containing buffer solution; the lower part of the piston moving chamber has a hole, so that the reagent chamber is connected to the quantitative chamber.

7. The integrated sample collection and detection device according to claim 6, characterized in that, The upper surface of the partition component is inclined with a lower center and a higher circumference.

8. The integrated sample collection and detection device according to claim 1 or 2, characterized in that, The reaction tube is a duckbill tube.

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