Microtome auxiliary device

Abstract

This invention belongs to the field of slide preparation technology, and particularly relates to a microscopic slide preparation auxiliary device, including a base plate, on which a support rod, a heating element, a slide carrier assembly, and a temperature measuring component are mounted. The support rod is vertically mounted on the base plate; the heating element, which is an alcohol lamp, is used to heat the slide; the slide carrier assembly is positioned above the heating element and is used to hold the slide; the slide carrier assembly can swing around the support rod to move the slide closer to or away from the heating element; the temperature measuring component is used to measure and display the temperature of the slide. This invention effectively ensures precise temperature control during the slide preparation process, maintains slide stability, improves the success rate of slide preparation, frees the operator's hands, and enhances microscopic slide preparation efficiency through standardized temperature control, while significantly reducing the risk of heat burns and ensuring the safety of experimental operations.

Description

Technical Field

[0001] This utility model belongs to the field of glass slide preparation technology, and in particular relates to a microscopic slide preparation auxiliary device. Background Technology

[0002] Microscopic observation of traditional Chinese medicine powder requires the preparation of chloral hydrate permeabilization slides. Procedure: Place a small amount of the powder to be observed on a clean glass slide. Add 1-2 drops of chloral hydrate solution to the slide and gently stir with a dissecting needle, ensuring the powder is fully in contact with the solution. Hold one end of the slide and heat it in the outer flame of an alcohol lamp. When the solution begins to boil slightly, remove it from the flame, add more chloral hydrate solution, and continue heating. Control the flame temperature during the permeabilization process to avoid overheating and evaporation of the solution. Repeat this process until the sample is clearly permeated. After permeation, allow the slide to cool. To prevent chloral hydrate crystallization, add 1-2 drops of dilute glycerol, then gently cover with a coverslip, ensuring the sample is evenly distributed and free of air bubbles. Then, place the prepared slide under a microscope to observe the clarity and transparency of cells or tissues, ensuring the permeation effect meets requirements.

[0003] Currently, in laboratory procedures, the heating and permeation process involves holding one end of a glass slide by hand and placing it in the outer flame of an alcohol lamp for heating. This method is prone to causing burns, the slide is easy to slip off the hand, and the heating temperature is difficult to control. Utility Model Content

[0004] The purpose of this invention is to address the aforementioned technical problems by providing a microscopy slide preparation auxiliary device. This device achieves the effects of maintaining slide stability, increasing the success rate of slide preparation, freeing the operator's hands, improving microscopy slide preparation efficiency through standardized temperature control, and significantly reducing the risk of thermal burns, thus ensuring the safety of experimental operations.

[0005] In view of this, the present invention provides a microscopic slide preparation auxiliary device, including a base plate, on which are provided:

[0006] A vertical pole is installed on the base plate.

[0007] A heating element, said heating element being used to heat a glass slide;

[0008] A slide assembly is disposed above the heating element. The slide assembly is used to hold a glass slide and can swing around the upright to move the glass slide closer to or away from the heating element.

[0009] A temperature measuring component is used to measure and display the temperature of a glass slide.

[0010] In this technical solution, 1-2 drops of chloral hydrate solution are added to a glass slide and gently stirred with a dissecting needle to ensure the powder is fully in contact with the solution. The slide is then placed on a slide assembly, and a heating element heats the slide. A temperature-measuring component monitors the slide temperature in real time. Since the micro-boiling temperature of the chloral hydrate permeation reagent is approximately 50°C, the temperature must be carefully monitored to prevent overheating and evaporation of the solution. If the temperature becomes too high or the liquid begins to micro-boil, the slide assembly is moved away from the heating element. After the temperature drops, it is moved back and reheated. This process is repeated until the sample is clearly permeated. After permeation, the slide is allowed to cool. To prevent chloral hydrate crystallization, 1-2 drops of dilute glycerol are added, and then a coverslip is gently placed on top, ensuring the sample is evenly distributed and free of air bubbles. This completes the slide preparation. This design effectively ensures precise temperature control during slide preparation, maintains slide stability, improves slide preparation success rate, frees up the operator's hands, enhances microscopy efficiency through standardized temperature control, significantly reduces the risk of thermal burns, and ensures experimental safety.

[0011] Furthermore, a connecting sleeve is connected to the outer periphery of the upright via a fixing structure, and a first positioning shaft is provided on the connecting sleeve parallel to the upright. The carrier assembly includes:

[0012] A slide carrier, one end of which is rotatably connected to a first positioning shaft, and the other end of which is provided with a slide portion for placing glass slides;

[0013] An operating unit is connected to a substrate and is used to operate the substrate to rotate around a first positioning axis.

[0014] Furthermore, the carrier portion is grooved, with an opening on the side of the groove away from the first positioning axis, and the bottom of the groove is hollowed out.

[0015] In this technical solution, a glass slide is placed so that it is pushed into the groove from the opening. The bottom of the groove is hollowed out to facilitate the heating element to heat the glass slide.

[0016] Furthermore, the fixing structure includes a locking screw hole opened on the connecting sleeve, and the locking knob is connected to the locking screw hole and abuts against the outer peripheral wall of the upright.

[0017] In this technical solution, loosening the locking knob allows the connecting sleeve to slide up and down on the upright. The operator can adjust the position of the slide assembly according to the height of the alcohol lamp flame and then tighten the locking knob.

[0018] Furthermore, the temperature measuring component includes a thermometer and a probe electrically connected to the thermometer. The probe is used to contact the upper surface of the glass slide, and the thermometer has a display screen that can display the temperature measured by the probe.

[0019] Furthermore, the temperature measuring component is mounted on the temperature measuring frame, and the temperature measuring frame is connected to the slide assembly via a rotating structure. The rotating structure is used to move the probe away from the slide. The temperature measuring frame is also equipped with a lifting component, which is used to raise the probe away from the surface of the slide or press it down against the surface of the slide.

[0020] In this technical solution, before the glass slide is placed into the slide section, the probe is far away from the slide section. After the glass slide is placed into the slide section, the probe is raised by the lifting component to prevent the probe from hitting the slide section when rotating. The temperature measuring frame is rotated by the rotating structure to make the probe rotate directly above the glass slide. Then the probe is reset and lowered to press against the upper surface of the glass slide, which facilitates temperature measurement and also prevents the glass slide from moving in the groove.

[0021] Furthermore, the rotating structure includes:

[0022] The second positioning shaft is vertically set on the upper surface of the carrier piece. One end of the temperature measuring frame is sleeved on the outer circumference of the second positioning shaft and rotatably connected to the second positioning shaft. The upper end of the second positioning shaft is provided with a threaded section, and a locking nut is threadedly connected to the outside of the threaded section.

[0023] In this technical solution, the locking nut needs to be loosened by rotating it so that the temperature measuring frame can rotate around the second positioning axis. After rotating it to be directly above the glass slide, the locking nut can be tightened to prevent the temperature measuring frame from rotating around the second positioning axis when the slide assembly is oscillating.

[0024] Furthermore, the lifting component includes:

[0025] A fixing rod extending from the temperature measuring frame;

[0026] An operating lever is positioned above a fixed rod and hinged to the middle of the fixed rod. An extension shaft is provided on the operating lever, and the probe wire is fixed on the extension shaft.

[0027] A reset spring is provided between the operating rod and the fixed rod, with the reset spring positioned on the side furthest from the probe at the junction of the fixed rod and the operating rod.

[0028] In this technical solution, the reset spring, in its natural state, supports the upper operating lever to press down the probe end. When it is necessary to raise the probe, the operator presses down the operating lever away from the probe, compressing the reset spring. The operating lever rotates around the hinge point to raise the probe. After the operator releases the hand, the reset spring extends and resets, pressing down the probe end.

[0029] Furthermore, the operating part is fixedly connected to the second positioning shaft.

[0030] Furthermore, the heating element is an alcohol lamp.

[0031] The beneficial effects of this utility model are:

[0032] 1. This design can effectively ensure precise temperature control during the slide preparation process, maintain the stability of the slide, improve the success rate of slide preparation, free the operator's hands, improve the efficiency of microscopy slide preparation through standardized temperature control, and significantly reduce the risk of thermal burns, ensuring the safety of experimental operations.

[0033] 2. Loosen the locking knob to allow the connecting sleeve to slide up and down on the upright. The operator can adjust the position of the slide assembly according to the height of the alcohol lamp flame before tightening the locking knob.

[0034] 3. Before the slide is placed into the slide section, the probe is kept away from the slide section. After the slide is placed into the slide section, the probe is raised by the lifting component to prevent the probe from hitting the slide section when it rotates. The temperature measuring frame is rotated by the rotating structure to make the probe rotate directly above the slide. Then the probe is reset and lowered so that it presses against the upper surface of the slide, which facilitates temperature measurement and also prevents the slide from moving in the groove. Attached Figure Description

[0035] Figure 1 This is a perspective view of the present invention;

[0036] Figure 2 This is a perspective view of the present invention from another angle;

[0037] Figure 3 This is an exploded view of this utility model;

[0038] Figure 4 This is a 3D view of the temperature sensing component;

[0039] The markings in the diagram are as follows:

[0040] 1. Base plate; 2. Upright pole; 3. Heating element; 4. Carrier assembly; 5. Temperature measuring assembly; 6. Connecting sleeve; 7. First positioning shaft; 8. Carrier component; 9. Carrier section; 10. Operating section; 11. Locking knob; 12. Groove; 13. Opening; 14. Thermometer; 15. Probe; 16. Display screen; 17. Lifting assembly; 18. Second positioning shaft; 19. Temperature measuring frame; 20. Locking nut; 21. Fixing rod; 22. Operating lever; 23. Extension shaft; 24. Return spring; 25. Hinge point; 26. Wire. Detailed Implementation

[0041] The technical solutions of the embodiments of this application will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application are within the scope of protection of this application.

[0042] In the description of this application, it should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. For ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following drawings denote similar items; therefore, once an item is defined in one drawing, it need not be further discussed in subsequent drawings.

[0043] It should be noted that the terms "first," "second," etc., used in the specification and claims of this application are used to distinguish similar objects and not to describe a specific order or sequence. It should be understood that such use of data can be interchanged where appropriate so that embodiments of this application can be implemented in orders other than those illustrated or described herein, and the objects distinguished by "first," "second," etc., are generally of the same class and are not limited in number; for example, a first object can be one or more. Furthermore, in the specification and claims, "and / or" indicates at least one of the connected objects, and the character " / " generally indicates that the preceding and following objects are in an "or" relationship.

[0044] It should be noted that in the description of this application, the directional terms such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description. Unless otherwise stated, these directional terms do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this application. The directional terms "inner" and "outer" refer to the inner and outer contours relative to the outline of each component itself.

[0045] It should be noted that, in this application, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element. Furthermore, it should be noted that the scope of the methods and apparatuses in the embodiments of this application is not limited to performing functions in the order shown or discussed, but may also include performing functions substantially simultaneously or in the reverse order, depending on the functions involved. For example, the described methods may be performed in a different order than described, and various steps may be added, omitted, or combined. Additionally, features described with reference to certain examples may be combined in other examples.

[0046] Example 1

[0047] like Figure 1-2 As shown, a microscopic slide preparation auxiliary device includes a base plate 1, on which:

[0048] Upright pole 2, which is vertically mounted on the base plate 1;

[0049] Heating element 3, which is used to heat the glass slide, is an alcohol lamp;

[0050] The slide assembly 4 is disposed above the heating element 3. The slide assembly 4 is used to place the glass slide. The slide assembly 4 can swing around the upright 2 to move the glass slide closer to or away from the heating element 3.

[0051] Temperature measuring component 5 is used to measure and display the temperature of the glass slide.

[0052] Add 1-2 drops of chloral hydrate solution to a glass slide and gently stir with a dissecting needle to ensure the powder is fully in contact with the solution. Then place the slide on slide assembly 4. Heating element 3 heats the slide, and temperature measuring element 5 monitors the slide temperature in real time. Since the slight boiling point of chloral hydrate permeation reagent is around 50℃, the temperature must be carefully monitored to prevent overheating and evaporation of the solution. If the temperature is too high or the solution begins to slightly boil, move slide assembly 4 away from heating element 3. After the temperature drops, move it back and reheat. Repeat this process until the sample is clearly permeated. After permeation, allow the slide to cool. To prevent chloral hydrate crystallization, add 1-2 drops of dilute glycerin, then gently cover with a coverslip, ensuring the sample is evenly distributed and free of air bubbles. This completes the slide preparation. This design effectively ensures precise temperature control during slide preparation, maintains slide stability, improves slide preparation success rate, frees up the operator's hands, enhances microscopy efficiency through standardized temperature control, significantly reduces the risk of thermal burns, and ensures experimental safety.

[0053] Example 2

[0054] like Figure 3 As shown, the outer periphery of the upright 2 is connected to a connecting sleeve 6 via a fixed structure. A first positioning shaft 7 is provided on the connecting sleeve 6, parallel to the upright. The carrier assembly 4 includes:

[0055] The slide carrier 8 has one end rotatably connected to the first positioning shaft 7 and the other end is provided with a slide carrier part 9 for placing glass slides.

[0056] Operation unit 10, which is connected to the carrier 8, is used to operate the carrier 8 to rotate around the first positioning axis 7.

[0057] The fixing structure includes a locking screw hole on the connecting sleeve 6, and a locking knob 11 connected to the locking screw hole and pressed against the outer peripheral wall of the upright 2. Loosening the locking knob 11 allows the connecting sleeve 6 to slide up and down on the upright 2. The operator can adjust the position of the carrier assembly 4 according to the height of the alcohol lamp flame and then tighten the locking knob 11.

[0058] Example 3

[0059] like Figure 1 As shown, the slide section 9 is shaped like a groove 12, with an opening 13 on the side of the groove 12 away from the first positioning axis 7, and the bottom of the groove 12 is hollowed out. A glass slide is placed so that it is pushed into the groove 12 from the opening 13. The hollow bottom of the groove 12 facilitates heating of the glass slide by the heating element 3.

[0060] Example 4

[0061] like Figure 2-4As shown, the temperature measuring component 5 includes a thermometer 14 and a probe 15 electrically connected to the thermometer 14. The probe 15 is used to contact the upper surface of the glass slide, and the thermometer 14 has a display screen 16 that can display the temperature measured by the probe 15.

[0062] The temperature measuring component 5 is mounted on the temperature measuring frame 19. The temperature measuring frame 19 is connected to the slide assembly 4 via a rotating structure. The rotating structure is used to move the probe 15 away from the slide. The temperature measuring frame 19 is also equipped with a lifting component 17, which is used to raise the probe 15 away from the surface of the slide or press it down against the surface of the slide.

[0063] Before the slide is placed into the slide section 9, the probe 15 is away from the slide section 9. After the slide is placed into the slide section 9, the probe 15 is raised by the lifting assembly 17 to prevent the probe 15 from hitting the slide section 9 when it rotates. The temperature measuring frame 19 is rotated by the rotating structure to make the probe 15 rotate directly above the slide. Then the probe 15 is reset and lowered to press against the upper surface of the slide, which facilitates temperature measurement and also prevents the slide from moving in the groove 12.

[0064] The rotating structure includes:

[0065] The second positioning shaft 18 is vertically disposed on the upper surface of the carrier piece 8. One end of the temperature measuring frame 19 is sleeved on the outer periphery of the second positioning shaft 18 and rotatably connected to the second positioning shaft 18. The upper end of the second positioning shaft 18 is provided with a threaded section, and a locking nut 20 is threadedly connected to the outside of the threaded section.

[0066] The locking nut 20 needs to be loosened by rotating it so that the temperature measuring frame 19 can rotate around the second positioning axis 18. After rotating it to be directly above the glass slide, the locking nut 20 can be tightened to prevent the temperature measuring frame 19 from rotating around the second positioning axis 18 when the slide assembly 4 is swaying.

[0067] The lifting component 17 includes:

[0068] A fixing rod 21 extends from the temperature measuring frame 19;

[0069] The operating lever 22 is located above the fixed rod 21 and is hinged to the middle of the fixed rod 21. An extension shaft 23 is provided on the operating lever 22, and the probe 15 wire 26 is fixed on the extension shaft 23.

[0070] A reset spring 24 is disposed between the operating rod 22 and the fixed rod 21, and the reset spring 24 is disposed on the side away from the probe 15 at the junction of the fixed rod 21 and the operating rod 22.

[0071] In its natural state, the return spring 24 supports the upper operating lever 22, causing the probe 15 to press down. When it is necessary to raise the probe 15, the operator presses down the operating lever 22 away from the probe 15, compressing the return spring 24. The operating lever 22 rotates around the hinge point 25, raising the probe 15. After the operator releases the hand, the return spring 24 extends and returns to its original position, causing the probe 15 to press down.

[0072] The operating part 10 is fixedly connected to the second positioning shaft 18.

[0073] The embodiments of this application have been described above with reference to the accompanying drawings. Unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other. This application is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of this application without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of this application.

Claims

1. A microtoming aid, characterized in that , including base plate (1), on which are provided: Upright pole (2), which is vertically mounted on the base plate (1); Heating element (3), said heating element (3) is used to heat the glass slide; The slide assembly (4) is disposed above the heating element (3). The slide assembly (4) is used to place the glass slide. The slide assembly (4) can swing around the upright (2) to move the glass slide closer to or away from the heating element (3). Temperature measuring component (5) is used to measure and display the temperature of the glass slide.

2. The device according to claim 1, wherein The outer periphery of the upright (2) is connected to a connecting sleeve (6) by a fixed structure. A first positioning shaft (7) is provided on the connecting sleeve (6) parallel to the upright. The carrier assembly (4) includes: The slide carrier (8) has one end rotatably connected to the first positioning shaft (7) and the other end is provided with a slide carrier part (9) for placing glass slides. The operation unit (10) is connected to the carrier (8) and is used to operate the carrier (8) to rotate around the first positioning axis (7).

3. A microslide preparation aid according to claim 2, wherein, The substrate (9) is in the shape of a groove (12), with an opening (13) on the side of the groove (12) away from the first positioning axis (7), and the bottom of the groove (12) is hollowed out.

4. The device according to claim 2, wherein The fixing structure includes a locking screw hole opened on the connecting sleeve (6), and the locking knob (11) is connected to the locking screw hole and abuts against the outer peripheral wall of the upright (2).

5. The device according to claim 3, wherein The temperature measuring component (5) includes a thermometer (14) and a probe (15) electrically connected to the thermometer (14). The probe (15) is used to contact the upper surface of the glass slide. The thermometer (14) has a display screen (16) that can display the temperature measured by the probe (15).

6. The microscopic slide preparation auxiliary device according to claim 5, characterized in that, The temperature measuring component (5) is set on the temperature measuring frame (19). The temperature measuring frame (19) is connected to the slide assembly (4) through a rotating structure. The rotating structure is used to move the probe (15) away from the slide. The temperature measuring frame (19) is also provided with a lifting component (17). The lifting component (17) is used to raise the probe (15) away from the surface of the slide or press it down against the surface of the slide.

7. A microslide preparation aid according to claim 6, wherein, The rotating structure includes: The second positioning shaft (18) is vertically set on the upper surface of the carrier piece (8). One end of the temperature measuring frame (19) is sleeved on the outer circumference of the second positioning shaft (18) and rotatably connected to the second positioning shaft (18). The upper end of the second positioning shaft (18) is provided with a threaded section, and a locking nut (20) is threaded on the outside of the threaded section.

8. A microslide preparation aid according to claim 7, wherein, The lifting component (17) includes: A fixing rod (21) extends from the temperature measuring frame (19); The operating lever (22) is located above the fixed rod (21) and is hinged to the middle of the fixed rod (21). An extension shaft (23) is provided on the operating lever (22) outward. The probe (15) wire (26) is fixed on the extension shaft (23). A reset spring (24) is provided between the operating rod (22) and the fixed rod (21). The reset spring (24) is located on the side away from the probe (15) at the junction of the fixed rod (21) and the operating rod (22).

9. A microslide preparation aid according to claim 8, wherein, The operation part (10) is fixedly connected with the second positioning shaft (18).

10. The device according to claim 1, wherein The heating element (3) is an alcohol lamp.

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