A microfluidic chip integrated device capable of multi-target detection

Abstract

The utility model discloses a kind of microfluidic chip integrated devices that can realize multi-target detection, it is related to microfluidic chip integrated device technical field, including integrated device ontology, multiple groups of signal reaction cavities are arranged on the integrated device ontology, each signal reaction cavity is correspondingly provided with waste liquid discharge pipe, chip insertion hole and sample inlet channel, and multiple groups of sample inlet channel path length are same, and are connected with a group of sample inlet tube.The utility model is based on micro-nano 3D printing technology, through innovative flow passage design, standardization interface integration and sealing optimization, solve the uneven sample distribution, signal interference and other problems in prior art, provide a reliable, portable device for multi-target biological toxin detection.

Description

Technical Field

[0001] This utility model relates to the field of microfluidic chip integrated device technology, and in particular to a microfluidic chip integrated device capable of multi-target detection. Background Technology

[0002] Electrochemical immunosensors, as a core component of microfluidic systems, can generate electrical signals through the binding of specific antibodies to targets, achieving highly sensitive detection. However, a previously established electrochemical immunosensor detection method for Clostridium perfringens α-toxin detection in the laboratory has certain limitations in terms of integration, ease of operation, and signal stability.

[0003] First, when using antibody-modified immunoelectrochemical sensors to detect contaminated samples, it is necessary to pre-modify the electrodes with corresponding specific antibodies for different toxins, followed by multiple rounds of detection. For applications involving the simultaneous detection of multiple possible toxins, the single-chip detection strategy increases operation time, thus failing to effectively improve the detection efficiency of the target toxin.

[0004] Furthermore, traditional electrochemical immunosensor detection methods expose the sensing area to the air, while the electrode sensing area is very sensitive. This will cause the electrode to capture abnormal signals more frequently, thus interfering with the interpretation of the results.

[0005] Traditional single-target toxin electrochemical immunosensor detection requires strict operation from the experimenter. If the uniformity of sample distribution cannot be maintained during the detection process, it will significantly affect the repeatability and accuracy of the positive electrode signal.

[0006] To address the aforementioned technical bottlenecks, it is necessary to develop a highly integrated, compact, and easy-to-operate microfluidic chip integrated device to achieve efficient and simultaneous detection of multiple target biotoxins. Utility Model Content

[0007] The purpose of this invention is to address the shortcomings of existing technologies by proposing a microfluidic chip integrated device capable of multi-target detection.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] A microfluidic chip integrated device capable of multi-target detection is characterized in that: it includes an integrated device body, on which multiple signal reaction chambers are provided, each signal reaction chamber is provided with a waste liquid discharge tube, a chip insertion hole and a sample injection channel, the multiple sample injection channels have the same path length and are connected to a set of sample injection tubes.

[0010] Furthermore, a chip insertion hole is provided on the first side of the signal reaction cavity, and a sample injection channel is provided on the second side of the signal reaction cavity, with the first side and the second side being arranged opposite to each other.

[0011] Furthermore, the waste liquid discharge tube has the same structure as the sample inlet tube. The sample inlet tube has a cylindrical structure and is adapted to the connecting tube of the syringe. Both the sample inlet tube and the waste liquid discharge tube are located on the end face of the integrated device body.

[0012] Furthermore, the waste liquid discharge pipe is provided in three sets, and the waste liquid discharge pipe is connected to the spiral injector through a 1-to-3 tube, a capillary tube, a male Luer adapter, and a double female Luer straight connector.

[0013] Furthermore, the chip has three insertion holes for configuring an electrochemical immunosensor with three functionalized specific biometric elements.

[0014] Furthermore, the signal reaction cavity is also provided with a chip encapsulation hole, which is used for injecting photocurable adhesive.

[0015] Beneficial effects

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] Based on micro-nano 3D printing technology, this invention solves the problems of uneven sample distribution and signal interference in the prior art through innovative flow channel design, standardized interface integration and sealing optimization, providing a reliable and portable device for the detection of multi-target biotoxins. Attached Figure Description

[0018] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0019] Figure 1 This is a schematic diagram of the overall structure of the microfluidic integrated device.

[0020] Figure 2 This is a schematic diagram of the structure after combining a microfluidic integrated device with a biochip.

[0021] Figure 3 This is a perspective view of the structure after combining a microfluidic integrated device with a biochip.

[0022] Figure 4 This is a perspective view of a microfluidic integrated device.

[0023] In the diagram: Sample inlet tube-1; Signal reaction chamber-2; Sample inlet channel-3; Waste liquid discharge tube-4; Chip insertion hole-5 and Chip packaging hole-6. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0025] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0026] Reference Figures 1-4 The main body is provided with multiple signal reaction chambers 2. Each signal reaction chamber 2 is provided with a waste liquid discharge pipe 4, a chip insertion hole 5 and a sample injection channel 3. The multiple sample injection channels 3 have the same path length and are connected to a sample injection tube 1.

[0027] The detection of multi-target biotoxins is achieved by integrating an electrochemical immunosensor with functionalized antibodies of different specificities. The sample inlet tube 1 is configured to receive the sample microfluidic; the signal reaction chamber 2 is configured as the region where the target contained in the sample undergoes a biological reaction with the functionalized specific antibodies on the chip; the sample inlet channel 3 is configured as the connecting pipe between the sample inlet and the signal reaction chamber 2; the waste liquid outlet tube 4 is configured as the outlet for waste liquid discharge at the end of the signal test; and the chip insertion hole 5 is configured as the junction between the electrochemical immunosensor with the functionalized specific antibodies and the integrated microfluidic chip device.

[0028] The sample introduction channel 3 is the connecting pipe between the sample introduction tube 1 and the signal reaction chamber 2, used to transport the sample solution to the reaction chamber for reaction. The sample introduction path has a special curved design to ensure that the sample can enter the signal reaction chamber 2 at the same time.

[0029] In other preferred embodiments, a chip insertion hole 5 is provided on the first side of the signal reaction cavity 2, and a sample injection channel 3 is provided on the second side of the signal reaction cavity 2, with the first side and the second side being arranged opposite to each other.

[0030] In other preferred embodiments, the waste liquid discharge tube 4 has the same structure as the sample inlet tube 1. The sample inlet tube 1 has a cylindrical structure and is adapted to the connecting tube of the syringe. Both the sample inlet tube 1 and the waste liquid discharge tube 4 are disposed on the end face of the integrated device body.

[0031] In other preferred embodiments, the waste liquid discharge pipe 4 is provided with three sets, and the waste liquid discharge pipe 4 is connected to the spiral injector through a 1-to-3 pipe, a capillary tube, a male Luer adapter and a female Luer straight connector.

[0032] In other preferred embodiments, there are three chip insertion holes 5 for configuring an electrochemical immunosensor with three functionalized specific biometric elements.

[0033] In other preferred embodiments, the signal reaction cavity 2 is further provided with a chip encapsulation hole 6, which is used to inject photocurable adhesive to increase the sealing of the signal reaction cavity 2 and thereby reduce the interference of noise signals.

[0034] Working principle and usage process of this utility model:

[0035] First, the model is printed using a 3D printer. Then, the model is assembled using a 1-to-3 tube, capillary tubing, ZCSJ-1 / 16 male Luer adapter, double female Luer straight connector, and spiral injector to achieve the function of multi-target detection.

[0036] Then, the assembled electrochemical immunosensors (coated with CPA, RT, and BoNT / A antibodies respectively) were inserted into the printed microfluidic model. The sensor was then encapsulated and fixed by injecting photocurable adhesive into the chip encapsulation holes. Subsequently, the assembled sample inlet and waste liquid outlet pipes were installed on the model device, thus completing the overall assembly of the model.

[0037] Finally, multi-target biotoxin detection was performed. Specifically, the sample to be tested was injected into the reaction chamber through the injection port. After an immunoassay of 15 minutes, the injection port was replaced with a syringe filled with ultrapure water to clean the reaction chamber. Waste liquid was discharged and collected through the waste liquid outlet. After cleaning, potassium ferricyanide test solution was injected through the injection port, and the signal was detected using an electrochemical workstation.

[0038] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A microfluidic chip integrated device capable of multi-target detection, characterized in that: The device includes an integrated device body, which is provided with multiple signal reaction chambers. Each signal reaction chamber is provided with a waste liquid discharge tube, a chip insertion hole and a sample injection channel. The multiple sample injection channels have the same path length and are connected to a sample injection tube.

2. The microfluidic chip integrated device capable of multi-target detection according to claim 1, characterized in that: The first side of the signal reaction cavity is provided with a chip insertion hole, and the second side of the signal reaction cavity is provided with a sample injection channel. The first side and the second side are arranged opposite to each other.

3. The microfluidic chip integrated device for multi-target detection according to claim 1, characterized in that: The waste liquid discharge tube has the same structure as the sample inlet tube. The sample inlet tube is cylindrical and is adapted to the connecting tube of the syringe. Both the sample inlet tube and the waste liquid discharge tube are located on the end face of the integrated device body.

4. The microfluidic chip integrated device for multi-target detection according to claim 1, characterized in that: The waste liquid discharge pipe is provided in three sets, and the waste liquid discharge pipe is connected to the spiral injector through a 1-to-3 tube, a capillary tube, a male Luer adapter and a female Luer straight connector.

5. A microfluidic chip integrated device capable of multi-target detection according to claim 1, characterized in that: The chip has three insertion holes for configuring an electrochemical immunosensor with three functionalized specific biometric elements.

6. The microfluidic chip integrated device for multi-target detection according to claim 1, characterized in that: The signal reaction cavity is also provided with a chip encapsulation hole, which is used to inject photocurable adhesive.

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