A micro-PCR reaction device

Abstract

The utility model relates to PCR reaction tube technical field, a trace PCR reaction device, including PCR reaction tube, the upper surface of PCR reaction tube is provided with lid, the inside of PCR reaction tube is provided with the trace reaction mechanism of being convenient for improving reaction speed and saving reagent, the trace reaction mechanism includes tube core, cone top, connecting piece and reaction cavity, the tube core sets up in the inside of PCR reaction tube, the cone top fixedly connected in the upper surface of tube core, the connecting piece fixedly connected in the lateral wall of tube core, the reaction cavity sets up between the inner wall of PCR reaction tube and tube core. This trace PCR reaction device, through the tube core top that connecting piece connects in the inside of PCR reaction tube still installs cone top, still sets up the reaction cavity between PCR reaction tube and tube core, in order to save reagent while improving reaction speed and effect.

Description

Technical Field

[0001] This utility model relates to the field of PCR reaction tube technology, specifically a micro-PCR reaction device. Background Technology

[0002] PCR (Polymerase Chain Reaction) is a technique used to amplify specific DNA fragments in vitro. With the development of PCR technology, new techniques such as fluorescent PCR, digital PCR, and microfluidic PCR are constantly being developed. It plays an increasingly important role in applications such as infectious disease diagnosis, genetic disease diagnosis, and tumor diagnosis. PCR reaction tubes are essential experimental consumables in PCR operations. They provide a closed, precisely controlled microenvironment for the PCR reaction.

[0003] The aforementioned devices lack the structure to reduce reagent usage while improving PCR reaction efficiency, resulting in the requirement that the amount of reagent or sample placed in a PCR reaction tube is typically 20-50 microliters. When the temperature changes, it takes a certain amount of time for the reaction system to reach equilibrium, leading to prolonged reaction time and poor reaction uniformity. This places high demands on the amount of reagent and sample used. Based on the shortcomings of existing technology, this invention designs a micro-PCR reaction device. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a micro-PCR reaction device that has the advantages of reducing reagent consumption while improving PCR reaction efficiency.

[0005] This utility model provides the following technical solution: a micro-PCR reaction device, including a PCR reaction tube, the upper surface of which is provided with a cap, and the inside of which is provided with a micro-reaction mechanism to facilitate improving the reaction speed and saving reagents;

[0006] A micro-reaction mechanism includes a core, a cone tip, a connector, and a reaction chamber. The core is disposed inside a PCR reaction tube, the cone tip is fixedly connected to the upper surface of the core, the connector is fixedly connected to the side wall of the core, and the reaction chamber is disposed between the inner wall of the PCR reaction tube and the core.

[0007] As a preferred embodiment of this utility model, a handle is fixedly connected to one side of the cover, a sealing inner cover is fixedly connected to the lower surface of the cover, a second sealing ring is fixedly connected to the outer wall of the sealing inner cover, a buckle is fixedly connected to the lower surface of the cover, a connecting strip is fixedly connected to the lower surface of the cover, and a fixing ring is fixedly connected to the other end of the connecting strip.

[0008] As a preferred embodiment of this utility model, a sealing ring is fixedly connected to the upper surface of the PCR reaction tube, a limiting ring is fixedly connected to the side wall of the PCR reaction tube, and a first sealing ring is fixedly connected to the inner wall of the PCR reaction tube.

[0009] As a preferred embodiment of this invention, the connector is fixedly connected to the inner wall of the PCR reaction tube.

[0010] As a preferred embodiment of this invention, the inner sealing cap is inserted into the top of the PCR reaction tube, and the second sealing ring is engaged with the first sealing ring.

[0011] In a preferred embodiment of this invention, the first sealing ring is positioned above the second sealing ring after being engaged with it.

[0012] In a preferred embodiment of this invention, the fixing ring is fitted onto the PCR reaction tube and positioned above the limiting ring.

[0013] As a preferred embodiment of this utility model, the buckle is movably engaged with the sealing ring.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. This micro-PCR reaction device, by setting up a core, distributes the reaction reagents near the inner wall of the PCR reaction tube, increasing the conduction efficiency with the PCR instrument's thermal module, improving the reaction speed, and greatly saving the amount of reaction reagents and sample requirements. By setting up a cone-shaped top and connectors, it is easy to fix the core while guiding the reagents dripping onto the top of the core into the reaction chamber. By setting up a reaction chamber, it is easy to distribute the reaction reagents or samples near the inner wall of the PCR reaction tube, avoiding the process of internal heat conduction in the liquid, improving the uniformity of the reaction system, and improving the PCR reaction efficiency.

[0016] 2. This micro-PCR reaction device is equipped with a lid, a handle, and a sealing inner cover to seal the top of the PCR reaction tube. A second sealing ring is provided, which is engaged with the first sealing ring, to prevent the lid and the sealing inner cover from popping open due to pressure changes during thermal cycling. A buckle is provided to further improve the stability of the sealing inner cover after it is closed. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0018] Figure 2 This is a schematic diagram of the main structure of the reaction tube of this utility model;

[0019] Figure 3 This is a schematic diagram of the unfolded structure of the lid and the inner sealing lid of this utility model;

[0020] Figure 4 This is a cross-sectional schematic diagram of the micro-reaction mechanism for PCR reaction tubes of this utility model;

[0021] Figure 5 This is a schematic diagram of the main body of the micro-reaction mechanism of this utility model.

[0022] In the diagram: 1. PCR reaction tube; 101. Sealing ring opening; 102. Limiting ring; 103. First sealing ring; 2. Cap; 201. Handle; 202. Sealing inner cap; 203. Second sealing ring; 204. Buckle; 205. Connecting strip; 206. Fixing ring; 3. Micro-reaction mechanism; 301. Core tube; 302. Conical tip; 303. Connector; 304. Reaction chamber. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figure 1-5 A micro-PCR reaction device includes a PCR reaction tube 1, a cap 2 on the upper surface of the PCR reaction tube 1, a micro-reaction mechanism 3 inside the PCR reaction tube 1 to facilitate increased reaction speed and save reagents, a sealing ring 101 fixedly connected to the upper surface of the PCR reaction tube 1, a limiting ring 102 fixedly connected to the side wall of the PCR reaction tube 1, and a first sealing ring 103 fixedly connected to the inner wall of the PCR reaction tube 1. The first sealing ring 103 is positioned above the second sealing ring 203 after being engaged.

[0025] The micro-reaction mechanism 3 includes a core 301, a cone 302, a connector 303, and a reaction chamber 304. The core 301 is disposed inside the PCR reaction tube 1. The cone 302 is fixedly connected to the upper surface of the core 301. The connector 303 is fixedly connected to the side wall of the core 301. The reaction chamber 304 is disposed between the inner wall of the PCR reaction tube 1 and the core 301. The connector 303 is fixedly connected to the inner wall of the PCR reaction tube 1.

[0026] By setting the core 301, the reaction reagents are distributed near the inner wall of the PCR reaction tube 1, which increases the conduction efficiency with the PCR instrument's thermal module, improves the reaction speed, and greatly saves the amount of reaction reagents and the sample quantity requirements. By setting the cone apex 302 and the connector 303, the core 301 is fixed and the reagents dripping onto the top of the core 301 are guided into the reaction chamber 304. By setting the reaction chamber 304, the reaction reagents or samples are distributed near the inner wall of the PCR reaction tube 1, avoiding the process of internal heat conduction in the liquid, improving the uniformity of the reaction system, and improving the PCR reaction efficiency.

[0027] A handle 201 is fixedly connected to one side of the lid 2. A sealing inner cover 202 is fixedly connected to the lower surface of the lid 2. A second sealing ring 203 is fixedly connected to the outer wall of the sealing inner cover 202. A buckle 204 is fixedly connected to the lower surface of the lid 2. A connecting strip 205 is fixedly connected to the lower surface of the lid 2. A fixing ring 206 is fixedly connected to the other end of the connecting strip 205. The sealing inner cover 202 is sealed and inserted into the top of the PCR reaction tube 1. The second sealing ring 203 is snapped into the first sealing ring 103. The fixing ring 206 is sleeved on the PCR reaction tube 1 and is positioned above the limiting ring 102. The buckle 204 is movably snapped into the sealing ring opening 101.

[0028] The lid 2, handle 201, and inner sealing cover 202 are provided to seal the top of the PCR reaction tube 1. The second sealing ring 203 is provided and engages with the first sealing ring 103 to prevent the lid 2 and inner sealing cover 202 from popping open due to pressure changes during thermal cycling. The buckle 204 is provided to further improve the stability of the inner sealing cover 202 after it is closed.

[0029] Working principle: When a micro-PCR reaction device is used, in the initial state, the sealing ring 101 is first set on the upper surface of the PCR reaction tube 1. The fixing ring 206 is fitted on the PCR reaction tube 1 and connected to the cover 2 through the connecting strip 205. The sealing inner cover 202 is set on the lower surface of the cover 2, and the sealing inner cover 202 can be inserted into the top of the PCR reaction tube 1 to seal the PCR reaction tube 1 and provide a stable and closed physical environment for the reaction experiment. Inside the PCR reaction tube 1, the top of the core 301 connected by the connector 303 is also installed with a cone 302. A reaction chamber 304 is also set between the PCR reaction tube 1 and the core 301 to save reagents while improving the reaction speed and effect.

[0030] When it is necessary to reduce the amount of reagent used and improve the efficiency of PCR reaction, first, turn the handle 201 upward to open the inner sealing cap 202 and the lid 2 from the top of the PCR reaction tube 1. Then, use the dispenser to slowly inject the reagent into the PCR reaction tube 1. If the reagent falls on the inner wall of the PCR reaction tube 1 after being injected into the PCR reaction tube 1, it will slide directly into the reaction chamber 304. If it drips onto the cone tip 302, the inclined surface of the cone tip 302 can be used to guide the reagent into the reaction chamber 304 until the required amount of reagent is reached. Then, stop injecting. At this time, put the inner sealing cap 202 back on the top of the PCR reaction tube 1 to seal it and lock it with the buckle 204. Finally, put it into the PCR instrument for reaction. This device can reduce the amount of reagent used while improving the efficiency of PCR reaction.

[0031] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A micro-PCR reaction apparatus, comprising a PCR reaction tube (1), characterized in that: The PCR reaction tube (1) is provided with a cap (2) on its upper surface, and the PCR reaction tube (1) is provided with a micro-reaction mechanism (3) inside to facilitate the increase of reaction speed and save reagents; The micro-reaction mechanism (3) includes a core (301), a cone (302), a connector (303), and a reaction chamber (304). The core (301) is disposed inside the PCR reaction tube (1). The cone (302) is fixedly connected to the upper surface of the core (301). The connector (303) is fixedly connected to the side wall of the core (301). The reaction chamber (304) is disposed between the inner wall of the PCR reaction tube (1) and the core (301).

2. The micro-PCR reaction apparatus according to claim 1, characterized in that: A handle (201) is fixedly connected to one side of the cover (2), a sealing inner cover (202) is fixedly connected to the lower surface of the cover (2), a second sealing ring (203) is fixedly connected to the outer wall of the sealing inner cover (202), a buckle (204) is fixedly connected to the lower surface of the cover (2), a connecting strip (205) is fixedly connected to the lower surface of the cover (2), and a fixing ring (206) is fixedly connected to the other end of the connecting strip (205).

3. The micro-PCR reaction apparatus according to claim 1, characterized in that: A sealing ring (101) is fixedly connected to the upper surface of the PCR reaction tube (1), a limiting ring (102) is fixedly connected to the side wall of the PCR reaction tube (1), and a first sealing ring (103) is fixedly connected to the inner wall of the PCR reaction tube (1).

4. The micro-PCR reaction apparatus according to claim 1, characterized in that: The connector (303) is fixedly connected to the inner wall of the PCR reaction tube (1).

5. A micro-PCR reaction apparatus according to claim 2, characterized in that: The sealing inner cap (202) is sealed and inserted into the top of the PCR reaction tube (1), and the second sealing ring (203) is engaged with the first sealing ring (103).

6. The micro-PCR reaction apparatus according to claim 3, characterized in that: The first sealing ring (103) is positioned above the second sealing ring (203) after they are engaged.

7. A micro-PCR reaction apparatus according to claim 2, characterized in that: The fixing ring (206) is fitted onto the PCR reaction tube (1) and is positioned above the limiting ring (102).

8. A micro-PCR reaction apparatus according to claim 2, characterized in that: The buckle (204) is movably engaged with the sealing ring (101).

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