A device for rapid detection of influenza virus

Abstract

The utility model relates to detection device technical field especially relates to a device for influenza virus rapid detection, it includes detection box, flap, placing seat, servo motor and prism etc., the detection box top has the flap rotation connection, the detection box bottom is installed with servo motor, servo motor output shaft has the prism coaxial connection, the placing seat inside is provided with the prism hole of the prism adaptation through servo motor drive's compound movement mechanism (circumferential rotation + axial lift) realizes the automatic mixing of sample and reagent, replaces traditional manual shaking operation, single batch can handle many test tubes, cooperates remote control system to realize quick operation, greatly shortens the detection period, solves the problem that traditional PCR operation is complex, time -consuming, satisfies the on -the -spot H1N1 quick screening demand of customs, port etc.

Description

Technical Field

[0001] This utility model relates to the field of detection device technology, and in particular to a device for rapid detection of influenza virus. Background Technology

[0002] In public health prevention and control, the H1N1 influenza virus is highly contagious and spreads rapidly, requiring rapid and accurate detection to block its transmission. However, existing technologies have significant shortcomings: RT-PCR, while accurate, relies on expensive equipment, is complex to operate, and is time-consuming, making it difficult to meet on-site needs; antigen detection is simple to operate but has low sensitivity and insufficient specificity, easily leading to missed or misdiagnosed cases; and virus isolation and culture are too time-consuming to be effective in emergency situations.

[0003] In recent years, the combined application of ERA (Enzyme Recombinant Amplification) and CRISPR-Cas13a technologies has opened up a new path for rapid H1N1 detection: ERA breaks through the dependence of traditional PCR on "precision temperature control equipment," and can complete gene amplification in 15-30 minutes at a constant temperature of 37-42℃ (the normal human body temperature range), naturally adapting to on-site detection; CRISPR-Cas13a can specifically recognize target genes, enabling immediate interpretation of detection results. However, the efficient synergy of the two still faces core challenges: the reaction system needs to be thoroughly mixed to ensure amplification and recognition efficiency; CRISPR-Cas13a is extremely sensitive to nucleic acid contamination; and the sample lysis stage must ensure the full release of viral nucleic acid. These conditions together constitute the key barriers to the practical application of the technology.

[0004] Existing detection devices are ill-suited to the aforementioned synergistic requirements. While most devices can accommodate the isothermal characteristics of ERA (whose temperature control precision requirements are far lower than those of PCR), they suffer from low mixing efficiency, lack of anti-contamination design, and incomplete sample lysis, preventing the full realization of the synergistic effect of ERA and CRISPR technologies. Therefore, developing a dedicated detection device that combines efficient mixing, anti-contamination, and ease of operation is crucial for improving the efficiency and accuracy of H1N1 on-site detection. Utility Model Content

[0005] In order to overcome the shortcomings mentioned in the background art, the present invention provides a device for rapid detection of influenza virus.

[0006] A device for rapid influenza virus detection includes a detection box, a flip plate, a placement seat, a servo motor, a prism, test tubes, test tube stoppers, a one-way tube, a placement bottle, a heater, a heating element, a protective shell, a temperature control component, and a toggle component. The top of the detection box is rotatably connected to the flip plate via a hinge. A servo motor is installed at the bottom inside the detection box, and the output shaft of the servo motor is coaxially connected to the prism. The placement seat has prism holes that fit the prism, and the placement seat slides into the prism through these holes and is placed inside the detection box. Multiple placement slots are spaced circumferentially within the placement seat, and each placement slot contains a sliding... The test tubes are placed on the test chamber, and the top of each test tube has a detachable stopper. A heater is embedded in the front of the test chamber, and a heating tube is connected to the heater. The heating tubes are arranged in a square pattern inside the test chamber and surround the outside of the test chamber. A protective shell is connected inside the test chamber to the outside of the heating tubes. A temperature control component is installed on the flip plate, and an actuating component is installed inside the protective shell. The actuating component includes a top block, which is installed on the front wall inside the protective shell. A ring-shaped mating groove is opened on the outer periphery of the test chamber. One side of the mating groove is inclined upward and the other side is inclined downward. The top block and the mating groove form a sliding guide fit.

[0007] To further explain, the constant temperature component includes a temperature detector and a controller. The controller and the temperature detector are installed sequentially from top to bottom on the front side of the flip plate. The detection end of the temperature detector extends into the detection box, and its signal output end is electrically connected to the heater control end. The servo motor, the temperature detector, and the heater are all electrically connected to the controller. The controller interacts with the remote control system through a wireless transmission module.

[0008] To further explain, the depth of the annular groove on the outer periphery of the placement seat is 3-5mm, and the groove wall is polished.

[0009] To further explain, it also includes support rods and pull rings. Multiple support rods are slidably connected circumferentially through the inner ring of the placement seat. The lower end of the support rod extends to the bottom of the corresponding test tube and abuts against the bottom wall of the test tube. A pull ring is hinged to the top of the support rod.

[0010] To further explain, it also includes transparent panels, which are embedded in the side walls of the testing box.

[0011] To further explain, a one-way tube is installed through the center of the test tube stopper, and a flexible placement bottle is detachably connected to the top end of the one-way tube.

[0012] The beneficial effects of this utility model are as follows: 1. This utility model realizes automatic mixing of samples and reagents through a composite motion mechanism (circumferential rotation + axial lifting) driven by a servo motor, replacing the traditional manual shaking operation. Multiple test tubes can be processed in a single batch. Combined with a remote control system, it can achieve rapid operation, greatly shorten the detection cycle, solve the problem of complex and time-consuming traditional PCR operation, and meet the needs of rapid H1N1 screening at customs, ports and other sites.

[0013] 2. This utility model, by incorporating a constant temperature component and combining a square array of heating tubes with a protective shell, stabilizes the temperature inside the detection chamber at 37-42℃±0.5℃. This precisely matches the optimal temperature requirements for ERA isothermal amplification and CRISPR-Cas13a activity, reducing the interference of temperature fluctuations on viral nucleic acid amplification efficiency and Cas13a targeted recognition specificity, thus providing a core guarantee for the accuracy of H1N1 detection. Attached Figure Description

[0014] Figure 1 This is a three-dimensional structural diagram of the present invention.

[0015] Figure 2 This is a three-dimensional structural diagram of the components of this utility model, including the testing box, the flip plate, and the placement seat.

[0016] Figure 3 This is a three-dimensional sectional view of the prism, test tube, and heater components of this utility model.

[0017] Figure 4 This is a three-dimensional sectional view of the components of this utility model, such as the support rod, pull ring, and top block.

[0018] Figure 5 This is a three-dimensional structural diagram of the components such as the groove, pull ring, and top block of this utility model.

[0019] Figure 6 This is a three-dimensional sectional view of the servo motor, pull ring, and mating groove components of this utility model.

[0020] The markings in the attached diagram are as follows: 1: Detection box, 2: Flip plate, 3: Placement seat, 4: Servo motor, 5: Prism, 6: Test tube, 601: Test tube stopper, 602: One-way tube, 603: Placement bottle, 7: Heater, 8: Heating tube, 9: Protective shell, 10: Temperature detector, 11: Controller, 12: Support rod, 13: Pull ring, 14: Top block, 15: Mating groove, 16: Transparent plate. Detailed Implementation

[0021] Example: A device for rapid detection of influenza virus, such as Figures 1-5As shown, the system includes a testing box 1, a flip plate 2, a placement seat 3, a servo motor 4, a prism 5, test tubes 6, test tube stoppers 601, a one-way tube 602, a placement bottle 603, a heater 7, a heating tube 8, a protective shell 9, a temperature control component, and a toggle component. The top of the testing box 1 is hinged to the flip plate 2, forming a flip-top sealed structure. The servo motor 4 is bolted to the bottom of the testing box 1, and the output shaft of the servo motor 4 is coaxially connected to the prism 5. The placement seat 3 has prism holes that fit the prism 5, and the placement seat 3 slides into the prism 5 through these holes and is placed inside the testing box 1. Multiple placement slots are spaced circumferentially inside the placement seat 3, each containing a test tube 6 for holding the sample to be tested and the reaction reagent. Each test tube 6 has a detachable test tube stopper 601 at its top, and a one-way tube 602 is installed through the center of the test tube stopper 601 for easy removal without disassembly. With the test tube stopper 601 in place, DNA polymerase is injected into the test tube 6. A flexible placement bottle 603 is detachably connected to the top end of the one-way tube 602, which stores DNA polymerase. The one-way tube 602 is a pipe structure with a built-in one-way valve, allowing only the liquid in the placement bottle 603 to flow into the one-way tube 602 in one direction. The bottom center of the placement seat 3 is provided with a reinforcing rib, which forms a ring support structure with the outer periphery of the prism hole, enhancing its resistance to deformation during movement. A heater 7 is embedded in the front of the detection box 1, and a heating tube 8 is connected to the heater 7. The heating tube 8 is distributed in a square shape inside the detection box 1 and surrounds the outside of the placement seat 3. A protective shell 9 is connected inside the detection box 1 at the position outside the heating tube 8. The protective shell 9 forms a wrap-around protection for the heating tube 8 to ensure that the heat energy is concentrated on the outer periphery of the placement seat 3. A constant temperature component is provided on the flip plate 2, and a toggle component is provided inside the protective shell 9.

[0022] like Figure 1 As shown, the constant temperature component includes a temperature detector 10 and a controller 11. The controller 11 and the temperature detector 10 are sequentially installed from top to bottom on the front side of the flip plate 2. The detection end of the temperature detector 10 extends into the detection box 1, and its signal output end is electrically connected to the control end of the heater 7. The servo motor 4, the temperature detector 10 and the heater 7 are all electrically connected to the controller 11. The controller 11 realizes data interaction with the remote control system through a wireless transmission module.

[0023] like Figures 4-5 As shown, the actuating assembly includes a top block 14. The top block 14 is bolted to the front wall inside the protective shell 9. The outer periphery of the placement seat 3 has an annular mating groove 15. One side of the mating groove 15 is inclined upward and the other side is inclined downward. The top block 14 and the mating groove 15 form a sliding guide fit. Through the wedge action of the two, the circumferential rotational motion of the placement seat 3 is converted into axial lifting motion. The depth of the mating groove 15 is 3-5mm. The groove wall is polished to reduce the frictional resistance when sliding with the top block 14 and ensure the smoothness of the axial lifting motion.

[0024] When performing rapid influenza virus testing, first remove the tube stopper 601, add the collected sample and lysis buffer to the tube 6 in the correct proportion, and then seal the tube 6 with the tube stopper 601 to prevent evaporation and contamination. Next, install the placement bottle 603 (containing heat-stable DNA polymerase, such as Bst DNA polymerase, suitable for isothermal PCR amplification systems) at the top port of the one-way tube 602 (the placement bottle 603 also contains primers, dNTP mixture, and buffer, forming a complete ERA reaction system). Then, insert the tubes 6 one by one into the placement slots of the placement seat 3, close the flap 2 to create a sealed space in the detection chamber 1, and proceed with the sample processing stage. Instructions are sent to the controller 11 via the remote control system. The servo motor 4 is activated, and its output shaft drives the prism 5 to rotate at a speed of 50-150 r / min. The prism 5, through its engagement with the prism hole, drives the placement seat 3 and the test tube 6 on it to rotate circumferentially. Simultaneously, the engagement between the groove 15 on the outer periphery of the placement seat 3 and the top block 14 forces the placement seat 3 to generate an axial lifting motion of 5-8 mm / s during rotation. This combined motion of circumferential rotation and axial lifting causes the liquid in the test tube 6 to continuously tumble, agitate, and collide, creating a continuous turbulent mixing effect. This effectively breaks up the static stratification of the sample and lysis buffer, ensuring full contact between the lysis buffer and the sample. By enhancing the relative motion between the liquids, the lysis buffer is encouraged to fully act on the H1N1 virus. The particles efficiently release viral RNA; this stage lasts 10-15 minutes. Next, pressing the placement bottle 603 causes the internal DNA polymerase and reaction components to burst through the valve of the one-way tube 602 and enter test tube 6 (the one-way tube 602 is designed to prevent backflow of liquid into test tube 6 and contamination of the enzyme preparation). At this point, the DNA polymerase will play a crucial role in the subsequent amplification stage: using cDNA generated from reverse transcription of viral RNA as a template and dNTPs in the reaction system as raw materials, it catalyzes the synthesis of daughter DNA strands according to the base pairing principle, achieving exponential amplification of the H1N1 target gene. Subsequently, the controller 11 activates the heater 7 and temperature detector 10, heating the tube. 8. The internal temperature of the detection chamber 1 is raised to 37-42℃ by thermal radiation. The temperature detector 10 collects temperature data in real time and feeds it back to the controller 11 to control the temperature within ±0.5℃. At the same time, the speed of the servo motor 4 is adjusted to 50-100r / min to make the test tube 6 shake slightly to ensure uniform distribution of the ERA reaction system and ensure efficient amplification of H1N1 target genes (such as HA gene fragments). This stage lasts for 15-20 minutes. After the amplification is completed, the controller 11 automatically cuts off the power to the heater 7, the servo motor 4 stops running, and the detection chamber 1 cools down naturally to room temperature (25±2℃). The flip plate 2 is opened to take out the test tube 6 for subsequent detection (such as product analysis, result interpretation, etc.).

[0025] like Figures 5-6As shown, it also includes a support rod 12 and a pull ring 13. Multiple support rods 12 are slidably connected circumferentially through the inner ring of the placement seat 3. The lower end of the support rod 12 extends to the bottom of the corresponding test tube 6 and abuts against the bottom wall of the test tube 6. The top of the support rod 12 is hinged to the pull ring 13 by a pin. When it is necessary to remove the test tube 6, the support rod 12 is driven to rise axially by pulling the pull ring 13 upward. The push action of the support rod 12 is used to lift the test tube 6 upward from the placement slot, so as to realize the convenient removal of the test tube 6. After the tube removal operation is completed, the pull ring 13 is released, and the support rod 12 returns to the initial position axially under its own gravity.

[0026] like Figures 1-2 As shown, it also includes a transparent plate 16. The transparent plate 16 is embedded in the left and right side walls of the test box 1. The transparent plate 16 is made of high light transmittance acrylic material. It forms a waterproof seal with the side wall of the test box 1 through the sealing ring. The transparent plate 16 can realize the visual monitoring of the operating status of the internal components, which is convenient for real-time observation and quick repair when abnormal situations such as jamming or abnormal noise occur.

Claims

1. A device for rapid detection of influenza virus, characterized in that: The components include a testing box (1), a flip plate (2), a placement seat (3), a servo motor (4), a prism (5), a test tube (6), a test tube stopper (601), a heater (7), a heating tube (8), a protective shell (9), a temperature control component, and a toggle component. The top of the testing box (1) is connected to the flip plate (2) via a hinge. The bottom of the testing box (1) is equipped with a servo motor (4). The output shaft of the servo motor (4) is coaxially connected to the prism (5). The placement seat (3) has a prism hole that fits the prism (5). The placement seat (3) slides with the prism (5) through the prism hole and is placed in the testing box. (1) Inside, multiple placement slots are spaced apart along the circumference inside the placement seat (3), and test tubes (6) are slidably placed in each placement slot. Each test tube (6) has a detachable test tube plug (601) at the top. A heater (7) is embedded in the front of the test box (1). A heating tube (8) is connected to the heater (7). The heating tubes (8) are distributed in a square shape inside the test box (1) and surround the outside of the placement seat (3). A protective shell (9) is connected inside the test box (1) at the position outside the heating tubes (8). A constant temperature component is provided on the flip plate (2), and a toggle component is provided inside the protective shell (9). The actuating component includes a top block (14). The top block (14) is installed on the front side wall inside the protective shell (9). The outer periphery of the placement seat (3) is provided with a ring-shaped mating groove (15). One side of the mating groove (15) is inclined upward and the other side is inclined downward. The top block (14) and the mating groove (15) form a sliding guide fit.

2. The device for rapid detection of influenza virus according to claim 1, characterized in that: The constant temperature component includes a temperature detector (10) and a controller (11). The controller (11) and the temperature detector (10) are installed sequentially from top to bottom on the front side of the flip plate (2). The detection end of the temperature detector (10) extends into the detection box (1), and its signal output end is electrically connected to the control end of the heater (7). The servo motor (4), the temperature detector (10) and the heater (7) are all electrically connected to the controller (11). The controller (11) realizes data interaction with the remote control system through the wireless transmission module.

3. The device for rapid detection of influenza virus according to claim 1, characterized in that: The annular groove (15) on the outer periphery of the placement seat (3) has a depth of 3-5mm, and the groove wall is polished.

4. A device for rapid detection of influenza virus according to claim 1, characterized in that: It also includes a support rod (12) and a pull ring (13). Multiple support rods (12) are slidably connected in a circumferential manner along the inner ring of the placement seat (3). The lower end of the support rod (12) extends to the bottom of the corresponding test tube (6) and abuts against the bottom wall of the test tube (6). A pull ring (13) is hinged to the top of the support rod (12).

5. A device for rapid detection of influenza virus according to claim 1, characterized in that: It also includes a transparent plate (16), and the transparent plate (16) is embedded in both sides of the detection box (1).

6. A device for rapid detection of influenza virus according to claim 1, characterized in that: A one-way tube (602) is installed through the center of the test tube stopper (601), and a flexible placement bottle (603) is detachably connected to the top end of the one-way tube (602).

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