Multiple-sealed leak-proof centrifuge tubes for fluorescence immunochromatography

Abstract

This utility model relates to the field of centrifuge tube technology, and in particular to a multi-sealed, leak-proof centrifuge tube for fluorescence immunochromatography. It includes a movable cover plate and a tube plug. The tube plug is fixedly connected below the movable cover plate, and the tube plug has an annular groove plate with several through holes. A sealing diaphragm is located below the annular groove plate and is fixedly connected to the tube plug. A first one-way valve is fixedly connected to the movable cover plate, and a three-way pipe is threaded to the input end of the first one-way valve. The input end of a second one-way valve is fixedly connected to one side of the three-way pipe. This utility model forms a sealed air chamber at the centrifuge tube opening through the first one-way valve, the second one-way valve, and the air inlet cylinder, effectively preventing liquid leakage and evaporation. The multi-sealing design effectively avoids sample leakage or loss of volatile components during centrifugation, ensuring stable fluorescent marker concentration, thereby reducing false negative or false positive results due to sample loss and improving experimental efficiency and economy.

Description

Technical Field

[0001] This utility model relates to the field of centrifuge tube technology, and in particular to a multi-sealed, leak-proof centrifuge tube for fluorescence immunochromatography. Background Technology

[0002] Fluorescence immunochromatographic centrifuge tubes are specialized consumables designed specifically for fluorescence immunoassay experiments. Primarily used for sample pretreatment and reagent preservation, these tubes are typically made of brown or light-proof materials to effectively prevent quenching of fluorescent markers due to light exposure, ensuring accurate results. During experiments, they can be used for centrifugation of biological samples such as blood to rapidly obtain plasma or serum components. Their special tube wall treatment process reduces non-specific adsorption of biomolecules, improving the sensitivity of subsequent fluorescence immunochromatographic assays. Some products are also sterilized and contain anticoagulants, allowing for direct use in the collection and preservation of clinical samples, facilitating applications such as point-of-care testing (POCT) and infectious disease screening. These centrifuge tubes play a vital role in food safety testing, medical diagnosis, and environmental monitoring, and are an indispensable key consumable in fluorescence immunochromatographic technology.

[0003] Traditional centrifuge tubes used in fluorescence immunochromatography have a simple sealing structure. However, long-term use can lead to seal failure, resulting in incomplete sealing and sample leakage, especially of volatile components. This directly affects the concentration or proportion of the fluorescently labeled material, causing inaccurate results. Furthermore, air or impurities may enter the centrifuge tube, interfering with the immunochromatographic reaction and leading to erroneous results. Inaccurate results necessitate repeating the process, wasting reagents, time, and sample, thus increasing costs.

[0004] Therefore, to address the problems of inaccurate test results, wasted reagents, time, and samples, and increased costs caused by the simple sealing structure and poor sealing of traditional fluorescent immunochromatographic centrifuge tubes, a multi-layered leak-proof fluorescent immunochromatographic centrifuge tube capable of preventing and detecting leaks can be designed to solve these problems. Utility Model Content

[0005] To overcome the problems of traditional fluorescent immunochromatographic centrifuge tubes having simple sealing structures, poor sealing leading to inaccurate test results, wasted reagents, time and samples, and increased costs, a multi-layered leak-proof fluorescent immunochromatographic centrifuge tube capable of preventing and detecting leaks can be designed.

[0006] The technical solution of this utility model is as follows: a multi-sealed, leak-proof centrifuge tube for fluorescence immunochromatography, including a movable cover plate; and a tube plug, with the tube plug fixedly connected below the movable cover plate, the tube plug having an annular groove plate with several through holes, a sealing diaphragm below the annular groove plate, the sealing diaphragm being fixedly connected to the tube plug, a first one-way valve fixedly connected to the movable cover plate, a three-way pipe threadedly connected to the input end of the first one-way valve, the input end of a second one-way valve fixedly connected to one side of the three-way pipe, an air inlet cylinder fixedly connected above the three-way pipe, and a piston slidably connected to the inner wall of the air inlet cylinder.

[0007] Preferably, when sealing the centrifuge tube, a negative pressure is generated by pumping the piston, making the pressure in the three-way pipe lower than atmospheric pressure. The second one-way valve opens, supplying gas into the three-way pipe. Then, the piston is pushed into the air inlet cylinder, outputting pressure so that the pressure inside the three-way pipe is greater than atmospheric pressure. This causes the second one-way valve to close and the first one-way valve to open, supplying gas between the tube plug and the sealing diaphragm. A high-pressure gas chamber is formed in the annular groove plate area through the through hole, ensuring the centrifuge tube's sealing performance.

[0008] Preferably, a pressure sensor is fixedly connected to the movable cover plate, and an analog gauge is fixedly connected above the pressure sensor. The pressure sensor and the analog gauge are electrically connected.

[0009] Preferably, the tube plug is provided with multi-stage conical grooves, and several first rubber gaskets are fixedly connected to the multi-stage conical grooves.

[0010] Preferably, an exhaust needle valve is fixedly connected above the movable cover plate, and a flexible connecting strip is fixedly connected to one side of the movable cover plate.

[0011] Preferably, the other end of the flexible connecting strip is fixedly connected to a fixed support, and the fixed support is fixedly connected to a fixed sleeve.

[0012] Preferably, the fixing sleeve is provided with a connecting key, and a second rubber washer is fixedly connected to the top of the fixing sleeve.

[0013] Preferably, a centrifuge tube is fixedly connected to the inner wall of the fixing sleeve, and the centrifuge tube is made of an opaque and smooth material.

[0014] The beneficial effects of this utility model are:

[0015] 1. By utilizing the first one-way valve, the second one-way valve, and the air inlet, a sealed air chamber is formed at the centrifuge tube opening, effectively preventing liquid leakage and evaporation. The multiple sealing design effectively avoids sample leakage or loss of volatile components during centrifugation, ensuring stable fluorescent label concentration, thereby reducing false negative or false positive results caused by sample loss, reducing experimental errors, and improving experimental efficiency and economy.

[0016] 2. A pressure sensor was installed for real-time detection to identify potential leaks in centrifuge tubes in advance, preventing leaks during experiments, improving safety, optimizing detection performance, reducing the risk of experimental failure, and minimizing resource waste. Attached Figure Description

[0017] Figure 1 The diagram shown is a schematic representation of the overall three-dimensional structure of this utility model.

[0018] Figure 2 The diagram shown is a schematic representation of the centrifuge tube structure of this utility model.

[0019] Figure 3 The diagram shown is a schematic representation of the tube plug structure of this utility model.

[0020] Figure 4 The diagram shown is a schematic representation of the first check valve of this utility model.

[0021] Figure 5 The diagram shown is a cross-sectional view of the movable cover plate of this utility model.

[0022] Explanation of reference numerals in the attached drawings: 1. Movable cover plate; 2. Pipe plug; 201. Multi-stage conical groove; 202. Annular groove plate; 2021. Through hole; 3. Sealing diaphragm; 4. First one-way valve; 5. T-connector; 6. Second one-way valve; 7. Air inlet cylinder; 8. Piston; 9. Pressure sensor; 10. Pointer gauge; 11. Exhaust needle valve; 12. First rubber gasket; 13. Flexible connecting strip; 14. Fixed support; 15. Fixed sleeve; 1501. Connecting key; 16. Second rubber gasket; 17. Centrifuge tube. Detailed Implementation

[0023] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0024] Please see Figures 1-5This utility model provides an embodiment of a multi-sealed, leak-proof centrifuge tube for fluorescence immunochromatography, comprising a movable cover plate 1 and a tube plug 2. The tube plug 2 is fixedly connected below the movable cover plate 1. The tube plug 2 has an annular groove plate 202 with several through holes 2021. A sealing diaphragm 3 is located below the annular groove plate 202 and is fixedly connected to the tube plug 2. A first one-way valve 4 is fixedly connected to the movable cover plate 1. A three-way pipe 5 is threadedly connected to the input end of the first one-way valve 4. The input end of a second one-way valve 6 is fixedly connected to one side of the three-way pipe 5. An air inlet cylinder 7 is fixedly connected to the centrifuge tube, and a piston 8 is slidably connected to the inner wall of the air inlet cylinder 7. When the centrifuge tube is sealed, a negative pressure is generated by pumping the piston 8, so that the pressure in the three-way pipe 5 is less than the atmospheric pressure. The second one-way valve 6 opens and supplies gas into the three-way pipe 5. Then, the piston 8 is pushed into the air inlet cylinder 7 to output pressure, so that the pressure inside the three-way pipe 5 is greater than the atmospheric pressure. This causes the second one-way valve 6 to close and the first one-way valve 4 to open, supplying gas between the tube plug 2 and the sealing diaphragm 3. A high-pressure gas chamber is formed in the area of ​​the annular groove plate 202 through the through hole 2021, ensuring the sealing of the centrifuge tube.

[0025] Please see Figures 3-5 In this embodiment, a pressure sensor 9 is fixedly connected to the movable cover plate 1, and a pointer meter 10 is fixedly connected above the pressure sensor 9. The pressure sensor 9 and the pointer meter 10 are electrically connected. The pressure sensor 9 is used to detect the pressure in the sealed air chamber in real time and transmit the detection signal to the pointer meter 10. By observing the pointer meter 10, it is determined whether there is a leak in the centrifuge tube. The pressure sensor 9 in this device is a BMP280 sensor. The tube plug 2 is provided with multi-stage conical grooves 201. Several first rubber gaskets 12 are fixedly connected to the multi-stage conical grooves 201. The multi-stage conical grooves 201 are used for connection, and the first rubber gaskets 12 on the multi-stage conical grooves 201 provide initial sealing for the centrifuge tube. An exhaust needle valve 11 is fixedly connected above the movable cover plate 1, and a flexible connecting strap 13 is fixedly connected to one side of the movable cover plate 1. Pressure is applied to the exhaust needle valve 11 to release the high-pressure gas in the air chamber, making it easy to remove the movable cover plate 1. The flexible connecting strap 13 is used to fix the movable cover plate 1.

[0026] Please see Figures 1-2In this embodiment, a fixed support 14 is fixedly connected to the other end of the flexible connecting strip 13. A fixed sleeve 15 is fixedly connected to the fixed support 14. The movable cover plate 1 is fixed to the fixed support 14 by the flexible connecting strip 13, and then the fixed support 14 is fixed to the fixed sleeve 15 to make the structure complete. A connecting key 1501 is provided on the fixed sleeve 15. A second rubber gasket 16 is fixedly connected to the top of the fixed sleeve 15. The connecting key 1501 on the fixed sleeve 15 facilitates the installation of centrifuge tubes. When the fixed sleeve 15 and the movable cover plate 1 are connected by the second rubber gasket 16, the sealing of the device is ensured. A centrifuge tube body 17 is fixedly connected to the inner wall of the fixed sleeve 15. The centrifuge tube body 17 is made of an opaque and smooth material. The fluorescent immunoassay reagent to be tested is placed in the centrifuge tube body 17. The opaque and smooth material avoids other interfering factors that may affect the experiment.

[0027] When sealing the centrifuge tube, the tube plug 2 is inserted into the centrifuge tube body 17 and connected using the multi-stage conical groove 201. The first rubber gasket 12 on the multi-stage conical groove 201 provides initial sealing to the centrifuge tube. Then, the second rubber gasket 16 seals the fixed sleeve 15 and the movable cover plate 1. Next, by pulling the piston 8, a negative pressure is generated, making the pressure in the three-way pipe 5 lower than atmospheric pressure. The second one-way valve 6 opens, supplying gas into the three-way pipe 5. Then, the piston 8 is pushed into the air inlet cylinder 7, outputting pressure so that the pressure inside the three-way pipe 5 is greater than atmospheric pressure. This causes the second one-way valve 6 to close and the first one-way valve 4 to open, supplying gas to the tube plug 2. Gas is supplied between the centrifuge tube and the sealing diaphragm 3. A high-pressure gas chamber is formed in the area of ​​the annular groove plate 202 through the through hole 2021 to ensure the sealing of the centrifuge tube. At the same time, the gas pressure sensor 9 is used to detect the gas pressure in the sealed gas chamber in real time and transmit the detection signal to the pointer gauge 10. By observing the pointer gauge 10, it is determined whether there is a leak in the centrifuge tube. The centrifuge tube is installed using the connecting key 1501 on the fixed sleeve 15. After the experiment is completed, pressure is applied to the exhaust needle valve 11 to release the high-pressure gas in the gas chamber, making it easy to remove the movable cover plate 1. The movable cover plate 1 is also fixed to the fixed support 14 by the flexible connecting strap 13 to make the structure intact and not easy to lose.

[0028] Through the above steps, a sealed air chamber is formed at the centrifuge tube opening using the first one-way valve 4, the second one-way valve 6, and the air inlet cylinder 7. This effectively prevents liquid leakage and evaporation. The multiple sealing design effectively avoids sample leakage or loss of volatile components during centrifugation, ensuring stable fluorescent label concentration. This reduces false negative or false positive results caused by sample loss, reduces experimental errors, and improves experimental efficiency and economy. A pressure sensor 9 is also included for real-time detection, identifying potential leaks in the centrifuge tube in advance, preventing leaks during the experiment, improving safety, optimizing detection performance, reducing the risk of experimental failure, and minimizing resource waste. This addresses the problem of traditional fluorescent immunochromatographic centrifuge tubes having simple sealing structures, poor sealing leading to inaccurate test results, wasted reagents, time, and samples, and increased costs. A multi-layered leak-proof fluorescent immunochromatographic centrifuge tube capable of preventing and detecting leaks can be designed to address this issue.

Claims

1. A fluorescent immunoassay centrifuge tube with multiple sealing leakproofness, comprising a movable cover plate (1); characterized in that: It also includes a pipe plug (2), a pipe plug (2) is fixedly connected below the movable cover plate (1), the pipe plug (2) is provided with an annular groove plate (202), the annular groove plate (202) is provided with several through holes (2021), a sealing diaphragm (3) is provided below the annular groove plate (202), the sealing diaphragm (3) is fixedly connected to the pipe plug (2), a first one-way valve (4) is fixedly connected to the movable cover plate (1), a three-way pipe (5) is threadedly connected to the input end of the first one-way valve (4), the input end of the second one-way valve (6) is fixedly connected to one side of the three-way pipe (5), an air inlet cylinder (7) is fixedly connected above the three-way pipe (5), and a piston (8) is slidably connected to the inner wall of the air inlet cylinder (7).

2. The multi-sealed, leak-proof centrifuge tube for fluorescence immunochromatography according to claim 1, characterized in that: A pressure sensor (9) is fixedly connected to the movable cover plate (1), and a pointer meter (10) is fixedly connected above the pressure sensor (9). The pressure sensor (9) and the pointer meter (10) are electrically connected.

3. The multi-seal leak-proof fluorescent immuno-chromatographic centrifuge tube according to claim 1, characterized in that: The plug (2) is provided with a multi-stage conical groove (201), and a number of first rubber gaskets (12) are fixedly connected to the multi-stage conical groove (201).

4. The multi-seal leak-proof fluorescent immuno-chromatographic centrifuge tube according to claim 1, characterized in that: An exhaust needle valve (11) is fixedly connected above the movable cover plate (1), and a flexible connecting strip (13) is fixedly connected to one side of the movable cover plate (1).

5. The multi-seal leak-proof fluorescent immuno-chromatographic centrifuge tube according to claim 4, characterized in that: The other end of the flexible connecting strip (13) is fixedly connected to a fixed support (14), and the fixed support (14) is fixedly connected to a fixed sleeve (15).

6. The multi-seal leak-proof fluorescent immuno-chromatographic centrifuge tube according to claim 5, characterized in that: The fixing sleeve (15) is provided with a connecting key (1501), and a second rubber gasket (16) is fixedly connected above the fixing sleeve (15).

7. The multi-seal leak-proof fluorescent immuno-chromatographic centrifuge tube according to claim 6, characterized in that: The centrifuge tube (17) is fixedly connected to the inner wall of the fixed sleeve (15). The centrifuge tube (17) is made of an opaque and smooth material.

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