Multilayer tandem microbial ATP detection device

Abstract

This utility model relates to the technical field of ATP detection devices and discloses a multi-layer tandem microbial ATP detection device, including: a sampling swab, a detection liquid tube, a multi-layer detection liquid storage and connecting tube, and a threaded sealing cap. A threaded sealing cap is provided on one side of the detection liquid tube, and a sampling swab is slidably connected to the inner side of the threaded sealing cap. A sampling swab is also provided on the inner side of the detection liquid tube. A multi-layer detection liquid storage and connecting tube assembly is disposed between the detection liquid tube and the threaded sealing cap. This assembly is used to simultaneously store different components of multiple detection liquids, which are connected in series but independent of each other. This utility model uses the multi-layer detection liquid storage and connecting tube assembly to store different detection liquid components or different reaction components in layers. The hollow swab connecting cavity with the threaded sealing cap, combined with a syringe or external tubing, facilitates sample cleaning. The threaded connection of the tandem tube and the sealing diaphragm ensure a seal, and the puncture groove facilitates operation.

Description

Technical Field

[0001] This utility model relates to the technical field of ATP detection devices, specifically a multi-layer tandem microbial ATP detection device. Background Technology

[0002] In many fields such as food safety, medical and health care, and environmental monitoring, the accurate and rapid determination of microbial quantity has always been a key link in ensuring safety and quality. Traditional microbial detection methods, such as microbial isolation, culture, and counting procedures, are not only cumbersome to operate and require professional personnel to perform strict aseptic operations in sterile laboratories, but also have a long detection cycle, often requiring 18 to 24 hours or even longer to obtain results. In real-world scenarios where every second counts, such as food processing workshops needing to quickly determine the surface hygiene of equipment to ensure timely and safe food production, or hospital operating rooms urgently needing to determine the environmental microbial contamination to prevent infection, traditional methods are inadequate.

[0003] ATP (adenosine triphosphate), a key molecule used for energy storage and transfer in all living cells, is widely present in bacteria, fungi, and plant and animal cells. ATP-based microbial detection technologies have emerged to address this need. These technologies utilize bioluminescence to infer the number of microorganisms by detecting the ATP content in a sample. While existing ATP detection devices have achieved rapid detection to some extent—for example, most ATP bacterial and microbial detectors can complete a single test within tens of seconds, greatly improving detection efficiency—these devices are generally single-detection structures. They cannot simultaneously address the ATP release and detection of different types and characteristics of microorganisms in complex samples. Microorganisms have diverse cell wall structures; bacteria, yeast, and fungi have relatively rigid cell walls, which differ significantly from somatic cells with their soft phospholipid bilayer membranes in terms of ATP release conditions. In actual complex environmental sample testing, a single detection structure cannot meet the optimal detection conditions for different microorganisms, thus limiting the accuracy and comprehensiveness of the detection results. Therefore, we propose a multi-layer tandem microbial ATP detection device. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a multi-layer tandem microbial ATP detection device, which solves the aforementioned problems.

[0005] To achieve the above-mentioned objectives, this utility model provides the following technical solution:

[0006] A multilayer tandem microbial ATP detection device includes:

[0007] The sample swab, the test liquid tube, and the threaded sealing cap are provided. A threaded sealing cap is provided on one side of the test liquid tube, and a sample swab is slidably connected to the inner side of the threaded sealing cap. A sample swab is also provided on the inner side of the test liquid tube.

[0008] A multi-layer test liquid storage and connection pipe assembly is installed between the test liquid pipe and the threaded sealing cap. The multi-layer test liquid storage and connection pipe assembly is used to simultaneously store different components of multiple test liquids, which are connected in series but independent of each other.

[0009] Preferably, one end of the sampling swab is fixedly connected to a swab head, and the sampling swab and the swab head are hollowly connected by a swab communication cavity.

[0010] Preferably, one end of the detection liquid tube is fixedly connected to a detection liquid tube connecting platform, a sealing diaphragm is fixedly connected to the inner side of the detection liquid tube connecting platform, and a fixed internal thread is provided on the inner side of the detection liquid tube connecting platform.

[0011] Preferably, the sealing diaphragm has a puncture-resistant groove on its side, which is used to easily and effortlessly puncture the sealing diaphragm.

[0012] Preferably, one end of the threaded sealing cap is fixedly connected to an external thread, the other side of the threaded sealing cap is fixedly connected to a Luer interface, the inner side of the Luer interface is provided with an internal thread, and the inner side of the threaded sealing cap is fixedly connected to an inner sealing diaphragm.

[0013] Preferably, the multilayer detection liquid storage and connection tube assembly includes a washing liquid tube, a binding liquid tube, a lysis liquid tube, and a sealing diaphragm. One side of the washing liquid tube is fixedly connected to a fixed external thread, and the other side of the washing liquid tube is fixedly connected to a washing liquid tube connection platform. The inner side of the washing liquid tube connection platform has a fixed internal thread. One side of the binding liquid tube is fixedly connected to a fixed external thread, and the other side of the binding liquid tube is fixedly connected to a binding liquid tube connection platform. The inner side of the binding liquid tube connection platform has a fixed internal thread. One side of the lysis liquid tube is fixedly connected to a fixed external thread, and the other side of the lysis liquid tube is fixedly connected to a lysis liquid tube connection platform. The inner side of the lysis liquid tube connection platform has a fixed internal thread.

[0014] Preferably, the inner thread of the fixed internal thread is connected to a fixed external thread, one end of the detection liquid tube is threaded to a washing liquid tube, the other end of the washing liquid tube is threaded to a binding liquid tube, the other end of the binding liquid tube is threaded to a lysis liquid tube, the inner thread of the fixed internal thread of the other end of the lysis liquid tube is connected to a connecting external thread, the other side of the lysis liquid tube is threaded to a threaded sealing cap, and the inner sides of the washing liquid tube connecting platform, the binding liquid tube connecting platform, and the lysis liquid tube connecting platform are fixedly connected to a sealing diaphragm.

[0015] Preferably, the number of multi-layer test liquid storage and connection tubes can be increased or decreased according to the specific test items, with a total number ≥ 1.

[0016] Preferably, the swab connecting cavity is fitted with a threaded sealing cap and used with a syringe or external tubing. The swab head contaminated with the sample is cleaned by pushing the syringe or squeezing the tubing to release the cleaning solution.

[0017] Compared with the prior art, the advantages of this utility model are:

[0018] A multi-layer tandem microbial ATP detection device is provided, which has the following beneficial effects:

[0019] This invention utilizes a multi-layered detection solution storage and connecting tube assembly design. Washing solution tubes can be installed in layers according to actual testing needs, enabling stratified detection of different types of microorganisms. For example, lysis solution tubes and binding solution tubes can be used sequentially to target microorganisms with different cell wall structures, improving the accuracy and comprehensiveness of complex sample detection. The hollow swab connecting cavity design of the sampling swab, combined with the puncture-resistant structure of the threaded sealing cap and Luer interface, facilitates the flow of washing solution into the swab head for extract cleaning, ensuring sample processing effectiveness. Each series tube is connected by fixed internal and external threads, combined with a sealing diaphragm, ensuring that each chamber is independently sealed and leak-proof. The puncture-resistant groove design makes puncturing the sealing diaphragm easier. The entire device eliminates the need for a centrifuge or complex operations; simply pushing the swab allows it to pass sequentially through each series tube into the detection solution tube, simplifying the testing process, avoiding cross-contamination during tube transfer, and improving the accuracy and consistency of sample collection. Furthermore, the detection solution tube can be directly inserted into a fluorescence detector for rapid detection. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0021] Figure 2 This is a schematic diagram showing the overall structure of this utility model disassembled;

[0022] Figure 3 This is a cross-sectional view of the overall structure of this utility model;

[0023] Figure 4 This is a sectional view of the overall structure of this utility model.

[0024] In the diagram: 1. Sampling swab; 2. Swab head; 3. Swab connecting cavity; 4. Detection fluid tube; 5. Detection fluid tube connector; 6. Washing fluid tube; 7. Washing fluid tube connector; 8. Binding fluid tube; 9. Binding fluid tube connector; 10. Lysis fluid tube; 11. Lysis fluid tube connector; 12. Threaded sealing cap; 13. Connecting internal thread; 14. Luer interface; 15. Fixed internal thread; 16. Sealing diaphragm; 17. Punctureable groove; 18. Fixed external thread; 19. Connecting external thread; 20. Inner sealing diaphragm. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] Please see Figure 1-4 This utility model provides a technical solution:

[0027] A multilayer tandem microbial ATP detection device includes:

[0028] The sample swab 1, the test liquid tube 4, and the threaded sealing cap 12 are provided on one side of the test liquid tube 4. The sample swab 1 is slidably connected to the inner side of the threaded sealing cap 12. The sample swab 1 is provided on the inner side of the test liquid tube 4.

[0029] A multi-layer test liquid storage and connection pipe assembly is installed between the test liquid pipe 4 and the threaded sealing cap 12. The multi-layer test liquid storage and connection pipe assembly is used to simultaneously store different components of multiple test liquids, which are connected in series but independent of each other.

[0030] Furthermore, a swab head 2 is fixedly connected to one end of the sampling swab 1, and a swab communication cavity 3 is provided in the hollow interior of the sampling swab 1 and the swab head 2.

[0031] Furthermore, a detection liquid tube connecting platform 5 is fixedly connected to one end of the detection liquid tube 4, a sealing diaphragm 16 is fixedly connected to the inner side of the detection liquid tube connecting platform 5, and a fixing internal thread 15 is provided on the inner side of the detection liquid tube connecting platform 5.

[0032] Furthermore, the sealing diaphragm 16 has a puncture groove 17 on its side, which is used to easily and effortlessly puncture the sealing diaphragm 16.

[0033] Furthermore, an external thread 19 is fixedly connected to one end of the threaded sealing cover 12 on its outer side, and a Luer interface 14 is fixedly connected to the other side of the threaded sealing cover 12. An internal thread 13 is provided on the inner side of the Luer interface 14, and an inner sealing diaphragm 20 is fixedly connected to the inner side of the threaded sealing cover 12.

[0034] Furthermore, the multilayer detection fluid storage and connection tube assembly includes a washing fluid tube 6, a binding fluid tube 8, a lysis fluid tube 10, and a sealing diaphragm 16. One side of the washing fluid tube 6 is fixedly connected to a fixed external thread 18, and the other side of the washing fluid tube 6 is fixedly connected to a washing fluid tube connection platform 7. The inner side of the washing fluid tube connection platform 7 is provided with a fixed internal thread 15. One side of the binding fluid tube 8 is fixedly connected to a fixed external thread 18, and the other side of the binding fluid tube 8 is fixedly connected to a binding fluid tube connection platform 9. The inner side of the binding fluid tube connection platform 9 is provided with a fixed internal thread 15. One side of the lysis fluid tube 10 is fixedly connected to a fixed external thread 18, and the other side of the lysis fluid tube 10 is fixedly connected to a lysis fluid tube connection platform 11. The inner side of the lysis fluid tube connection platform 11 is provided with a fixed internal thread 15.

[0035] Furthermore, the inner thread of the fixed internal thread 15 is connected to the fixed external thread 18. One end of the detection liquid tube 4 is threaded to the washing liquid tube 6, the other end of the washing liquid tube 6 is threaded to the binding liquid tube 8, the other end of the binding liquid tube 8 is threaded to the pyrolysis liquid tube 10, the other end of the pyrolysis liquid tube 10 is threaded to the inner thread of the fixed internal thread 15 and connected to the connecting external thread 19, and the other side of the pyrolysis liquid tube 10 is threaded to the threaded sealing cap 12. The inner sides of the washing liquid tube connecting platform 7, the binding liquid tube connecting platform 9 and the pyrolysis liquid tube connecting platform 11 are fixedly connected to the sealing diaphragm 16. Through the function of the multi-layer detection liquid storage and connecting tube assembly, the washing liquid tube 6 can be installed in layers and tested in layers according to the actual detection requirements.

[0036] Furthermore, the number of multi-layer test liquid storage and connection tube assemblies can be increased or decreased according to specific test items, with a total number ≥ 1.

[0037] Furthermore, the swab connecting cavity 3 is fitted with a threaded sealing cap 12, which, in conjunction with a syringe or an external tubing, allows the cleaning solution propelled by pushing the syringe or squeezing the tubing to clean the swab head 2, which has been contaminated with the sample.

[0038] Structural Description:

[0039] Sampling swab 1: One end is fixed with swab head 2, and the interior forms a hollow swab communication cavity 3 with swab head 2 for collecting samples and allowing the cleaning solution to flow;

[0040] Swab head 2: Fixed to the top of sampling swab 1, and used in conjunction with swab connecting cavity 3 to complete sample collection and cleaning;

[0041] Swab connecting cavity 3: a hollow channel inside the sampling swab 1 and swab head 2, allowing the cleaning solution to flow in from the tail and out from the head to clean the sample;

[0042] Detection liquid tube 4: contains the final test sample, one end is connected to the detection liquid tube connector 5, and the inner side can hold the sampling swab 1 for insertion into the fluorescence detector for detection;

[0043] Detection liquid tube connection platform 5: fixed to one end of the detection liquid tube 4, with a sealing diaphragm 16 and a fixing internal thread 15 on the inside, used to connect the washing liquid tube 6;

[0044] Washing liquid tube 6: a basic unit for multi-layer detection liquid storage and connection tube assembly, with a fixed external thread 18 on one side and a washing liquid tube connection platform 7 on the other side, which can hold swabs and washing liquid.

[0045] Washing liquid pipe connecting platform 7: fixed to one end of washing liquid pipe 6, with a fixing internal thread 15 and a sealing diaphragm 16 on the inside, used to connect adjacent series pipes;

[0046] Binding fluid tube 8: One side has a fixed external thread 18, and the other side has a binding fluid tube connecting platform 9. The inside is filled with binding fluid to treat ATP in the sample.

[0047] Binding fluid tube connecting platform 9: fixed to one end of the binding fluid tube 8, with a fixing internal thread 15 and a sealing diaphragm 16 on the inner side, used to connect the pyrolysis fluid tube 10.

[0048] Lysis tube 10: One side has a fixed external thread 18, and the other side has a lysis tube connection platform 11. The inside is filled with lysis fluid to rupture microbial cells and release ATP.

[0049] 11: fixed to one end of 10 pyrolysis tube, with internal thread 15 and sealing diaphragm 16 on the inside, and the other end connected to threaded sealing cap 12 via thread.

[0050] Threaded sealing cap 12: One end has an external thread 19 connecting to the pyrolysis liquid pipe connecting platform 11, and the other side has a Luer interface 14 and an inner sealing diaphragm 20.

[0051] Luer interface 14: fixed to one side of threaded sealing cap 12, with internal thread 13 on the inside for connecting extraction tube or syringe to inject / extract liquid;

[0052] Fixed internal thread 15: It is opened on the inner side of the test liquid pipe connection table 5 and each series pipe connection table, and cooperates with the fixed external thread 18 to realize the component thread connection;

[0053] Sealing diaphragm 16: fixed to the inner side of the test liquid tube connection platform 5 and each series tube connection platform, with a puncture groove 17 on the surface, which is punctured when the swab passes through to maintain the chamber seal;

[0054] Punctureable groove 17: It is formed on the surface of the sealing diaphragm 16 to reduce the resistance of the swab to puncture the diaphragm and make the puncture operation more convenient;

[0055] Fixed external thread 18: Fixed to one side of the washing liquid pipe 6, the binding liquid pipe 8, and the pyrolysis liquid pipe 10, and cooperates with the fixed internal thread 15 to realize the component connection;

[0056] External thread 19: Fixed to one end of the threaded sealing cap 12, and threadedly connected to the internal thread 15 of the pyrolysis tube connecting platform 11.

[0057] Inner sealing diaphragm 20: fixed inside the threaded sealing cap 12, punctured when the swab passes through, to ensure the seal between the threaded sealing cap and the swab;

[0058] Working principle: When this utility model is needed, a sample is collected through the swab head 2 of the sampling swab 1. The sampling swab 1 has a hollow swab connecting cavity 3 inside. Then, the multi-layer detection liquid storage and connecting tube assembly (such as washing liquid tube 6, binding liquid tube 8, lysis liquid tube 10, etc.) between the threaded sealing cap 12 and the detection liquid tube 4 is assembled as required. Each series tube is connected by a fixed internal thread 15 and a fixed external thread 18, and is sealed by a sealing diaphragm 16 in the middle. Then, the sampled swab is inserted into the threaded sealing cap 12, and the swab is pushed to pass through each series tube in sequence. When passing through the membrane, the sealing diaphragm... The puncture groove 17 on the membrane 16 allows the swab to puncture the septum more easily. If sample cleaning is required during the pushing process, an extraction tube or syringe can be connected through the Luer interface 14 on the threaded sealing cap 12 to allow liquid to flow out from the swab head 2 through the swab connecting cavity 3 to clean the sample. When the swab passes through the lysis buffer tube 10, the lysis buffer will act on the sample, causing the microbial cells to rupture and release ATP. When passing through the binding fluid tube 8, the sample can be further processed. Finally, the swab reaches the detection fluid tube 4. Inserting the detection fluid tube downwards into the fluorescence detector can detect the ATP content, thereby inferring the number of microorganisms.

[0059] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A multilayer tandem microbial ATP detection device, characterized in that, include: The sample swab (1), the test liquid tube (4), and the threaded sealing cap (12) are provided on one side of the test liquid tube (4). The sample swab (1) is slidably connected to the inner side of the threaded sealing cap (12). The sample swab (1) is provided on the inner side of the test liquid tube (4). A multi-layer detection liquid storage and connection tube assembly is provided between the detection liquid tube (4) and the threaded sealing cap (12). The multi-layer detection liquid storage and connection tube assembly is used to simultaneously store different components of multiple layers of detection liquid, which are connected in series but independent of each other.

2. The multilayer tandem microbial ATP detection device according to claim 1, characterized in that, One end of the sampling swab (1) is fixedly connected to a swab head (2), and the sampling swab (1) and the swab head (2) are hollow and have a swab communication cavity (3).

3. The multilayer tandem microbial ATP detection device according to claim 1, characterized in that, One end of the detection liquid tube (4) is fixedly connected to a detection liquid tube connecting platform (5), and a sealing diaphragm (16) is fixedly connected to the inner side of the detection liquid tube connecting platform (5). A fixed internal thread (15) is provided on the inner side of the detection liquid tube connecting platform (5).

4. The multilayer tandem microbial ATP detection device according to claim 3, characterized in that, The sealing diaphragm (16) has a puncture groove (17) on its side, which is used to easily and effortlessly puncture the sealing diaphragm (16).

5. The multilayer tandem microbial ATP detection device according to claim 3, characterized in that, One end of the threaded sealing cap (12) is fixedly connected to an external thread (19), and the other side of the threaded sealing cap (12) is fixedly connected to a Luer interface (14). The inner side of the Luer interface (14) is provided with an internal thread (13), and the inner side of the threaded sealing cap (12) is fixedly connected to an inner sealing diaphragm (20).

6. The multilayer tandem microbial ATP detection device according to claim 5, characterized in that, The multilayer detection liquid storage and connection tube assembly includes a washing liquid tube (6), a binding liquid tube (8), a lysis liquid tube (10), and a sealing diaphragm (16). One side of the washing liquid tube (6) is fixedly connected to a fixed external thread (18), and the other side of the washing liquid tube (6) is fixedly connected to a washing liquid tube connection platform (7). The inner side of the washing liquid tube connection platform (7) is provided with a fixed internal thread (15). One side of the binding liquid tube (8) is fixedly connected to a fixed external thread (18), and the other side of the binding liquid tube (8) is fixedly connected to a binding liquid tube connection platform (9). The inner side of the binding liquid tube connection platform (9) is provided with a fixed internal thread (15). One side of the lysis liquid tube (10) is fixedly connected to a fixed external thread (18), and the other side of the lysis liquid tube (10) is fixedly connected to a lysis liquid tube connection platform (11). The inner side of the lysis liquid tube connection platform (11) is provided with a fixed internal thread (15).

7. The multilayer tandem microbial ATP detection device according to claim 6, characterized in that, The inner thread of the fixed internal thread (15) is connected to the fixed external thread (18). One end of the detection liquid tube (4) is connected to the washing liquid tube (6). The other end of the washing liquid tube (6) is connected to the binding liquid tube (8). The other end of the binding liquid tube (8) is connected to the lysis liquid tube (10). The inner thread of the fixed internal thread (15) at the other end of the lysis liquid tube (10) is connected to the connecting external thread (19). The other side of the lysis liquid tube (10) is connected to the threaded sealing cap (12). The inner sides of the washing liquid tube connecting platform (7), the binding liquid tube connecting platform (9), and the lysis liquid tube connecting platform (11) are fixedly connected to the sealing diaphragm (16).

8. The multilayer tandem microbial ATP detection device according to claim 6, characterized in that, The number of multi-layer test liquid storage and connection tubes can be increased or decreased according to the specific test items, with a total number ≥ 1.

9. The multilayer tandem microbial ATP detection device according to claim 6, characterized in that, The swab connecting cavity (3) is fitted with a threaded sealing cap (12) and used with a syringe or external tubing. The swab head (2) that has been contaminated with the sample is cleaned by pushing the syringe or squeezing the tubing to release the cleaning solution.

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