A multi-layered gas-permeable culture bottle
By designing a multi-layered aeration culture flask, the gas exchange area is increased by utilizing the bottom aeration membrane and the inner aeration membrane, which solves the problem of limited gas exchange efficiency in existing bottom aeration culture flasks and achieves increased cell density.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHANGHAI JINYU BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-07-31
- Publication Date
- 2026-06-30
Smart Images
Figure CN224430606U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell culture technology, and in particular to a multi-layered gas-permeable culture flask. Background Technology
[0002] In recent years, with the rapid development of the cell therapy industry, the demand for cell culture, as an important part of the cell therapy drug production process, has also been increasing. Among the culture containers for suspension cells, bottom-permeable culture flasks have been widely used by many cell production companies due to their high gas exchange efficiency and large amplification rate after cell culture.
[0003] However, in current bottom-permeable culture containers, the density of cultured cells depends on the area of the bottom permeable membrane. Due to the limitation of the bottom area of the culture flask, it is difficult to improve the gas exchange efficiency, so the density of cultured cells can only reach a certain level and cannot be increased further. Only more culture flasks can be added. The purpose of this application is to propose a culture flask with higher gas exchange efficiency. Utility Model Content
[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a multi-layered, breathable culture bottle.
[0005] To achieve the above objectives, the present invention adopts the following technical solution:
[0006] A multi-layer breathable culture bottle includes a bottle body and a support frame. The bottle body is mounted on a support base within the support frame, and a bottom breathable membrane is provided at the connection between the bottle body and the support base. A bottle cap is provided at the top of the bottle body. A single or multiple culture racks are provided inside the bottle body. Internal supports are provided around the culture racks, and an air exchange layer is provided on the internal supports. An internal breathable membrane is provided on the air exchange layer, and the air exchange layer around the culture racks communicates with an internal air exchange channel located in the middle of the culture racks for air exchange. The internal air exchange channel is connected to the environment below the support base for air exchange.
[0007] Preferably, the support base is used to support the bottom breathable membrane, and multiple ventilation holes are evenly arranged on the support base corresponding to the position of the bottom breathable membrane.
[0008] Preferably, the bottle cap is screwed and sealed to the bottle body by threads, and a sealing gasket is provided at the connection between the bottle cap and the bottle body.
[0009] Preferably, a filter membrane may be provided on the bottle cap to provide an air exchange channel between the culture bottle and the environment.
[0010] Preferably, the cap is configured as a bottle cap with a connecting pipe for adding, changing, and draining liquid from the culture bottle.
[0011] Preferably, the bottle contains cell culture medium, and the culture rack holds cells to be cultured.
[0012] The beneficial effects of this utility model are as follows:
[0013] In this invention, air exchange is achieved through the bottom permeable membrane and the internal permeable membrane of the culture rack, which increases the surface area for cell air exchange in the culture flask. This allows for the provision of oxygen for the growth of more cells, improves the air exchange efficiency in the culture flask, and increases the cell density in a single culture flask. Attached Figure Description
[0014] Figure 1 This is a schematic diagram of the structure of a multi-layered breathable culture bottle proposed in this utility model;
[0015] Figure 2 This is a schematic diagram of the internal multi-layer culture rack proposed in this utility model.
[0016] In the diagram: 1. Support frame, 2. Support base, 3. Bottom breathable membrane, 4. Bottle body, 5. Bottle cap, 6. Sealing gasket, 7. Filter membrane, 8. Cell culture medium, 9. Cells, 10. Culture rack, 11. Internal breathable membrane, 12. Air exchange layer, 13. Internal support, 14. Internal air exchange channel. Detailed Implementation
[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.
[0018] In Example 1, refer to Figure 1-2 A multi-layer permeable culture flask includes a flask body 4 and a support frame 1. The flask body 4 is mounted on a support seat 2 inside the support frame 1, and a bottom permeable membrane 3 is provided at the connection between the flask body 4 and the support seat 2. The support seat 2 is used to support the bottom permeable membrane 3, and multiple air exchange holes are evenly arranged on the support seat 2 corresponding to the position of the bottom permeable membrane 3, so as to provide an air exchange channel for the bottom permeable membrane 3 when culturing cells in the culture flask.
[0019] The top of the bottle body 4 is provided with a bottle cap 5, which is screwed and sealed to the bottle body 4 by threads. A sealing gasket 6 is provided at the connection between the bottle cap 5 and the bottle body 4 to increase the airtightness of the culture bottle. A filter membrane 7 can be provided on the bottle cap 5 to provide a ventilation channel between the culture bottle and the environment.
[0020] The bottle body 4 is provided with a single or multiple culture racks 10. The culture rack 10 is provided with internal supports 13 on all four sides. An air exchange layer 12 is provided on the internal support 13. An internal breathable membrane 11 is provided on the air exchange layer 12. The air exchange layer 12 on all four sides of the culture rack 10 is connected to the internal air exchange channel 14 located in the middle of the culture rack 10 for air exchange. The internal air exchange channel 14 is connected to the support base below and communicates with the environment for air exchange.
[0021] The bottle body 4 contains cell culture medium 8. During cell culture, cells 9 settle to the bottom permeable membrane 3 and the inner permeable membrane 11 by gravity. The bottom permeable membrane 3 and the inner permeable membrane 11 can prevent the cell culture medium 8 or cells 9 from leaking out during cell culture, and can also prevent external microorganisms from entering. At the same time, they can allow oxygen and carbon dioxide to pass through, thus meeting the gas exchange requirements during cell culture.
[0022] In Example 1, before cell culture, such as Figure 1 As shown, under sterile conditions, open the cap 5 of the pre-assembled multilayer aerated culture flask and add cells 9, cell culture medium 8, and other solvents required for cell culture into the culture flask. After adding the liquid, manually shake the culture flask to mix the liquid evenly, and then place it in the incubator to stand. After standing, the cells slowly sink to the bottom aerated membrane 3 and the inner aerated membranes 11 of each layer.
[0023] Afterwards, the cells enter the culture state. During the culture process, the bottom layer of cells 9 can exchange air through the bottom permeable membrane 3 to provide the oxygen required for cell growth and to release the carbon dioxide produced during the growth process. Oxygen and carbon dioxide are exchanged with the ambient gas through the holes on the support 2.
[0024] The cells 9 on the internal breathable membrane 11 of the internal culture rack 10 exchange air through the internal breathable membrane 11, providing the oxygen required for the growth of the cells 9 and expelling the carbon dioxide produced during the growth process. Oxygen and carbon dioxide are exchanged with the ambient gas through the air exchange layer 12 and the internal air exchange channel 14.
[0025] After the culture is complete, the cap 5 can be opened, the upper cell culture medium 8 can be extracted first, the remaining liquid containing cells can be shaken well, and then all of it can be extracted to complete one cell culture cycle.
[0026] In Example 2, unlike Example 1, the bottle cap 5 is equipped with a connecting pipe for adding, changing, and draining liquid from the culture bottle, enabling operation within a closed system without opening the cap and meeting the aseptic requirements of the operation.
[0027] In this invention, air exchange is achieved through the bottom permeable membrane 3 and the internal permeable membrane 11 of the culture rack 10, which increases the area for cell air exchange in the culture flask, provides the oxygen required for more cell growth, improves the air exchange efficiency in the culture flask, and increases the cell density cultured in a single culture flask.
[0028] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.
Claims
1. A multi-layered aerated culture flask, comprising a flask body (4) and a support frame (1), characterized in that, The bottle body (4) is mounted on a support base (2) inside the support frame (1), and a bottom breathable membrane (3) is provided at the connection between the bottle body (4) and the support base (2). A bottle cap (5) is provided at the top of the bottle body (4). A single or multiple culture racks (10) are provided inside the bottle body (4). An internal support (13) is provided around the culture rack (10). An air exchange layer (12) is provided on the internal support (13). An internal breathable membrane (11) is provided on the air exchange layer (12). The air exchange layer (12) around the culture rack (10) is connected to the internal air exchange channel (14) located in the middle of the culture rack (10) for air exchange. The internal air exchange channel (14) is connected to the environment below the support base (2) for air exchange.
2. The multi-layered gas-permeable culture flask according to claim 1, characterized in that, The support base (2) is used to support the bottom breathable membrane (3), and multiple ventilation holes are evenly arranged on the support base (2) corresponding to the position of the bottom breathable membrane (3).
3. The multi-layered gas-permeable culture flask according to claim 1, characterized in that, The bottle cap (5) is screwed and sealed to the bottle body (4) by threads, and a sealing gasket (6) is provided at the connection between the bottle cap (5) and the bottle body (4).
4. A multi-layered aerated culture flask according to claim 3, characterized in that, A filter membrane (7) can be provided on the cap (5) to provide an air exchange channel between the culture bottle and the environment.
5. A multi-layered aerated culture flask according to claim 3, characterized in that, The cap (5) is equipped with a bottle cap with a connecting pipe for adding, changing and draining liquid from the culture bottle.