High-throughput cell culture device for tumor drug screening

Abstract

This utility model discloses a high-throughput cell culture device for tumor drug screening, comprising: an incubator with a microplate having multiple microwells inside; a drug delivery component including multiple drug delivery needles; a temperature control component including a first and a second semiconductor cooling chip; a monitoring component including an optical imaging system and a fluorescence detection system; and a controller. The drug delivery component, temperature control component, and monitoring component are all controlled by the controller. The incubator provides a stable culture environment, the multiple microwells of the microplate enable high-throughput cell culture, improving screening efficiency, the drug delivery component precisely delivers drugs to each microwell, the temperature control component regulates the culture temperature, the monitoring component monitors cell status in real time, and the controller coordinates the operation of each component to achieve automated operation, thus facilitating the cell culture process for tumor drug screening.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology for tumor drugs, specifically a high-throughput cell culture device for tumor drug screening. Background Technology

[0002] Cancer, as a major disease that seriously threatens human health, has always been a key research focus in the fields of medicine and pharmacy in the development of its treatments. Drug screening is a crucial step in the cancer drug development process, enabling the rapid and accurate identification of drugs with potential therapeutic value from a large pool of compounds, thus providing a foundation for subsequent clinical trials and drug development.

[0003] Traditional methods for screening cancer drugs mainly rely on low-throughput cell culture and detection technologies, which have many limitations:

[0004] In cell culture, most traditional culture devices are single-mode, which makes it difficult to culture a large number of samples at the same time. This is not only inefficient, but also increases the possibility of experimental errors. Moreover, traditional culture devices do not have precise control over the culture environment. Temperature, humidity and other conditions are prone to fluctuations, which cannot provide a stable and suitable growth environment for cells, thus affecting the growth status of cells and the accuracy of experimental results.

[0005] In the drug addition process, traditional methods usually involve manual addition, which is not only cumbersome and slow, but also makes it difficult to ensure the accuracy and consistency of drug addition. Differences in drug addition between different operators may lead to deviations in experimental results and affect the reliability of drug screening.

[0006] For monitoring cell state, traditional techniques mainly rely on manual microscopic observation and simple biochemical detection methods. Manual observation is not only labor-intensive and inefficient, but also easily affected by subjective factors, making it difficult to capture subtle changes in cells in a timely and accurate manner.

[0007] With the deepening of tumor research and the ever-increasing demand for drug development, traditional low-throughput cell culture and screening methods can no longer meet the requirements of modern drug development. Therefore, developing a cell culture device for tumor drug screening that can achieve high-throughput cell culture, precise drug delivery, stable environmental control, and real-time monitoring is of great practical significance. Utility Model Content

[0008] The purpose of this invention is to provide a high-throughput cell culture device for tumor drug screening, which solves the problem that existing tumor drug screening methods mainly rely on low-throughput cell culture and detection technologies, resulting in many limitations.

[0009] To achieve the above objectives, the main technical solution adopted by this utility model includes: a high-throughput cell culture device for tumor drug screening, comprising: a culture chamber with a door rotatably mounted on one side, a microplate detachably mounted inside the culture chamber, the microplate having a plurality of microwells for cell culture for tumor drug screening; a drug delivery component including a plurality of drug delivery needles, each drug delivery needle having its outlet aligned with one of the microwells; and a temperature control component including a first semiconductor refrigeration chip and a second semiconductor refrigeration chip fixedly mounted on the inner wall of the culture chamber.

[0010] As a preferred technical solution, the following are also included:

[0011] The monitoring component includes an optical imaging system and a fluorescence detection system, which are movably mounted above the microplate.

[0012] The controller controls the dosing component, the temperature control component, and the monitoring component.

[0013] As a preferred technical solution, a mounting bracket is fixedly installed on the inner wall of the incubator, and the mounting bracket is provided with a snap-fit ​​groove, and the microporous plate is fitted and inserted into the snap-fit ​​groove;

[0014] Rubber pads are fixedly installed on both sides of the inner wall of the snap-fit ​​groove, and the rubber pads are in contact with the outer side of the microporous plate.

[0015] As a preferred technical solution, the drug delivery component further includes a first hydraulic cylinder fixedly installed on the top of the incubator. The telescopic end of the first hydraulic cylinder extends into the interior of the incubator and is fixedly connected to a first mounting plate. A plurality of first delivery tubes are fixedly installed at the bottom of the first mounting plate. A plurality of drug delivery needles are respectively connected to the plurality of first delivery tubes. One end of the first delivery tube is fixedly connected to a third delivery tube through a second delivery tube. One end of the plurality of third delivery tubes is fixedly connected to a fourth delivery tube. The fourth delivery tube is fixedly installed on the top of the incubator, and the upper end of the fourth delivery tube extends out of the outside of the incubator and is connected to the drug supply system. A drug delivery pump is fixedly installed on the fourth delivery tube.

[0016] The controller is electrically connected to the first hydraulic cylinder and the drug delivery pump, respectively.

[0017] As a preferred technical solution, the first semiconductor refrigeration chip and the second semiconductor refrigeration chip are symmetrically installed on both sides of the inner wall of the incubator. The hot end of the first semiconductor refrigeration chip faces the inside of the incubator and the cold end faces the outside of the incubator. The cold end of the second semiconductor refrigeration chip faces the inside of the incubator and the hot end faces the outside of the incubator.

[0018] A temperature sensor is fixedly installed on the inner wall of the incubator, and the controller is electrically connected to the first semiconductor refrigeration chip, the second semiconductor refrigeration chip, and the temperature sensor.

[0019] As a preferred technical solution, a condenser plate is fixedly installed at the cold end of the second semiconductor refrigeration chip;

[0020] The bottom of the incubator is equipped with a water collection box with an open top, and the condenser plate is located above the opening of the water collection box.

[0021] As a preferred technical solution, an atomizer and a humidity sensor are fixedly installed on the inner wall of the incubator, and the controller is electrically connected to the atomizer and the humidity sensor respectively.

[0022] As a preferred technical solution, the incubator has a mounting cavity on one side, and the monitoring component is placed inside the mounting cavity;

[0023] The monitoring component also includes a second hydraulic cylinder fixedly installed on the outer wall of the mounting cavity. The telescopic end of the second hydraulic cylinder extends into the interior of the mounting cavity and is fixedly connected to a connecting plate. A third hydraulic cylinder is fixedly connected to one end of the connecting plate. A second mounting plate is fixedly connected to the telescopic end of the third hydraulic cylinder. The optical imaging system and the fluorescence detection system are fixedly installed at the bottom of the second mounting plate.

[0024] The controller is electrically connected to the second hydraulic cylinder, the third hydraulic cylinder, the optical imaging system, and the fluorescence detection system, respectively.

[0025] As a preferred technical solution, a pull ring is fixedly installed at one end of the microporous plate.

[0026] As a preferred technical solution, both the incubator and the door are made of visible glass.

[0027] As a preferred technical solution, the mounting bracket has a hollow structure.

[0028] This utility model has at least the following beneficial effects:

[0029] This invention provides a high-throughput cell culture device for tumor drug screening. An incubator is used to provide a stable culture environment, and multiple microwells of a microplate can realize high-throughput cell culture, thereby improving screening efficiency.

[0030] The dosing unit can accurately add drugs to each microwell, achieving quantitative and timed dosing. The controller controls the electrical connection between the first hydraulic cylinder and the drug delivery pump, which automates the dosing process, reduces human error, improves the accuracy and consistency of dosing, and provides reliable dosing conditions for drug screening experiments.

[0031] The temperature control component regulates the culture temperature, which can precisely maintain the temperature inside the incubator within the range suitable for cell growth, providing a stable temperature environment for cell culture and helping to improve the accuracy of drug screening experimental results.

[0032] The monitoring component monitors cell status in real time, comprehensively acquires cell culture information, and can detect cell growth and drug effects in real time;

[0033] The controller coordinates the operation of all components, enabling automated operation and facilitating the cell culture process for tumor drug screening. Attached Figure Description

[0034] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings:

[0035] Figure 1 This is a three-dimensional view of the high-throughput cell culture device for tumor drug screening according to the present invention.

[0036] Figure 2 This is a front cross-sectional view of the incubator of the high-throughput cell culture device for tumor drug screening according to this utility model.

[0037] Figure 3 This is a schematic diagram of the microplate installation of the high-throughput cell culture device for tumor drug screening according to this utility model;

[0038] Figure 4 This is a schematic diagram of the dosing needle installation for the high-throughput cell culture device for tumor drug screening of this invention.

[0039] Explanation of icon numbers:

[0040] 1. Incubator; 101. Door; 102. Mounting cavity; 103. Water collection box; 2. Mounting frame; 201. Snap-fit ​​groove; 202. Rubber pad; 3. Microplate; 301. Micropore; 302. Pull ring; 4. First hydraulic cylinder; 401. First mounting plate; 5. First delivery tube; 501. Dosing needle; 502. Second delivery tube; 503. Third delivery tube; 504. Fourth delivery tube; 505. Drug delivery pump; 6. First semiconductor refrigeration chip; 601. Temperature sensor; 7. Second semiconductor refrigeration chip; 701. Condensing plate; 8. Nebulizer; 801. Humidity sensor; 9. Second hydraulic cylinder; 901. Connecting plate; 902. Third hydraulic cylinder; 903. Second mounting plate; 904. Optical imaging system; 905. Fluorescence detection system; 10. Controller. Detailed Implementation

[0041] The following will describe in detail the implementation of this application with reference to the accompanying drawings and embodiments, so that the implementation process of how this application uses technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0042] Example

[0043] Please refer to Figures 1 to 4 As shown, this embodiment provides a high-throughput cell culture device for tumor drug screening, including: a culture chamber 1, with a door 101 rotatably mounted on one side; a microplate 3 detachably mounted inside the culture chamber 1, the microplate 3 having a plurality of microwells 301 for cell culture for tumor drug screening; a drug delivery component, including a plurality of drug delivery needles 501, each drug delivery needle 501 having its outlet aligned with a microwell 301; a temperature control component, including a first semiconductor cooling pad 6 and a second semiconductor cooling pad 7 fixedly mounted on the inner wall of the culture chamber 1; and a monitoring component, including an optical imaging system 904 and a fluorescence... The light detection system 905, optical imaging system 904, and fluorescence detection system 905 are movably mounted above the microplate 3. The controller 10 controls the drug delivery component, temperature control component, and monitoring component. The incubator 1 provides a stable culture environment. The multiple wells 301 of the microplate 3 enable high-throughput cell culture, improving screening efficiency. The drug delivery component can precisely deliver drugs to each well 301. The temperature control component regulates the culture temperature, and the monitoring component monitors the cell status in real time. The controller 10 coordinates the work of all components to achieve automated operation, facilitating the cell culture process for tumor drug screening.

[0044] The incubator 1 has a mounting frame 2 fixedly installed on its inner wall. The mounting frame 2 has a snap-fit ​​groove 201, and the microplate 3 is inserted into the snap-fit ​​groove 201. Rubber pads 202 are fixedly installed on both sides of the inner wall of the snap-fit ​​groove 201. The rubber pads 202 are in contact with the outer side of the microplate 3. The snap-fit ​​groove 201 on the mounting frame 2 facilitates the insertion and connection of the microplate 3, making the operation simple. The rubber pads 202 increase the friction between the microplate 3 and the snap-fit ​​groove 201, preventing the microplate 3 from shaking and ensuring that the microplate 3 is placed stably during the operation of the device.

[0045] The dosing component also includes a first hydraulic cylinder 4 fixedly installed on the top of the incubator 1. The telescopic end of the first hydraulic cylinder 4 extends into the interior of the incubator 1 and is fixedly connected to a first mounting plate 401. Multiple first delivery pipes 5 are fixedly installed at the bottom of the first mounting plate 401. Several dosing needles 501 are respectively connected to the multiple first delivery pipes 5. One end of each first delivery pipe 5 is fixedly connected to a third delivery pipe 503 via a second delivery pipe 502. One end of each third delivery pipe 503 is fixedly connected to a fourth delivery pipe 504. The fourth delivery pipe 504 is fixedly installed on the top of the incubator 1, and its upper end extends out of the incubator 1 and connects to the drug supply system. A drug delivery pump 505 is fixedly installed on the fourth delivery pipe 504. The controller 10 is electrically connected to the first hydraulic cylinder 4 and the drug delivery pump 505. The connection is achieved by controlling the raising and lowering of the dosing needle 501 via the first hydraulic cylinder 4, precisely positioning it above the micro-orifice 301. The drug delivery pump 505 accurately delivers the drug to the dosing needle 501 through a series of delivery tubes, realizing quantitative and timed drug delivery. The controller 10 electrically connects and controls the first hydraulic cylinder 4 and the drug delivery pump 505, automating the drug delivery process, reducing human error, improving the accuracy and consistency of drug delivery, and providing reliable drug delivery conditions for drug screening experiments. The fourth delivery tube 504 connects to the drug supply system, facilitating the replacement of different types of drugs to meet the needs of screening various tumor drugs. The drug supply system can be of different types of drug tanks, and the drug delivery pump 505 delivers the drug solution inside the drug tank to the micro-orifice 301. In addition, the third delivery tube 503 is a flexible tube that works in conjunction with the raising and lowering operation of the dosing needle 501.

[0046] In this incubator 1, a first thermoelectric cooler 6 and a second thermoelectric cooler 7 are symmetrically mounted on opposite sides of the inner wall of the incubator 1. The hot end of the first thermoelectric cooler 6 faces the inside of the incubator 1, and the cold end faces the outside of the incubator 1. The cold end of the second thermoelectric cooler 7 faces the inside of the incubator 1, and the hot end faces the outside of the incubator 1. A temperature sensor 601 is fixedly mounted on the inner wall of the incubator 1. The controller 10 is electrically connected to the first thermoelectric cooler 6, the second thermoelectric cooler 7, and the temperature sensor 601. The first thermoelectric cooler 6 and the second thermoelectric cooler 7 are symmetrically mounted with their hot and cold ends facing each other. Depending on the direction, the temperature inside the incubator can be flexibly adjusted based on the information fed back by the temperature sensor 601. When the temperature is too low, the hot end of the first semiconductor cooling chip 6 is used for heating; when the temperature is too high, the cold end of the second semiconductor cooling chip 7 is used for cooling. This bidirectional adjustment mechanism can precisely maintain the temperature inside the incubator 1 within a suitable range for cell growth, providing a stable temperature environment for cell culture and helping to improve the accuracy of drug screening experimental results. In addition, the temperature sensor 601 monitors the temperature in real time and feeds the data back to the controller 10 to achieve real-time temperature control and ensure temperature stability.

[0047] The second semiconductor cooling chip 7 has a condenser plate 701 fixedly installed at its cold end. A water collection box 103 with an open top is placed at the bottom of the incubator 1. The condenser plate 701 is located above the opening of the water collection box 103. The condenser plate 701 at the cold end of the second semiconductor cooling chip 7 can condense water vapor in the air into water droplets, which drip into the water collection box 103. This effectively reduces the humidity in the incubator 1 and prevents excessive humidity from adversely affecting cell culture. The water collection box 103 is designed to collect condensate in a concentrated manner, making it easy to clean and maintain a clean environment inside the incubator 1. It also prevents excessive condensate from accumulating in the incubator, damaging other components, and extending the service life of the device.

[0048] The incubator 1 is equipped with an atomizer 8 and a humidity sensor 801 fixedly installed on its inner wall. The controller 10 is electrically connected to the atomizer 8 and the humidity sensor 801. The atomizer 8 increases the humidity inside the incubator 1. The humidity sensor 801 monitors the humidity in real time and feeds it back to the controller 10. When the humidity is below the set range, the controller 10 controls the atomizer 8 to work to supplement the humidity. When the humidity is too high, it is adjusted in conjunction with the dehumidification measures of the condenser plate 701. This humidity balance regulation mechanism can provide a suitable humidity environment for cell culture, ensure the normal growth and metabolism of cells, improve the reliability of drug screening experiments, and adapt to different cell culture needs.

[0049] The incubator 1 has a mounting cavity 102 on one side, and the monitoring component is placed inside the mounting cavity 102. The monitoring component also includes a second hydraulic cylinder 9 fixedly mounted on the outer wall of the mounting cavity 102. The telescopic end of the second hydraulic cylinder 9 extends into the mounting cavity 102 and is fixedly connected to a connecting plate 901. One end of the connecting plate 901 is fixedly connected to a third hydraulic cylinder 902. The telescopic end of the third hydraulic cylinder 902 is fixedly connected to a second mounting plate 903. An optical imaging system 904 and a fluorescence detection system 905 are fixedly mounted on the bottom of the second mounting plate 903. The controller 10 is electrically connected to the second hydraulic cylinder 9, the third hydraulic cylinder 902, the optical imaging system 904, and the fluorescence detection system 905 respectively. The positions of the optical imaging system 904 and the fluorescence detection system 905 can be flexibly adjusted through the second hydraulic cylinder 9 and the third hydraulic cylinder 902 so that they can monitor the microwells on the microplate and comprehensively acquire cell culture information. The controller 10 uniformly controls each component to realize the automation and precision of the monitoring process, which facilitates comprehensive and dynamic observation of cell status.

[0050] One end of the microplate 3 is fixedly equipped with a pull ring 302. The pull ring 302 makes it easier for operators to remove the microplate 3 from the mounting frame 2. Whether replacing the microplate 3, observing cell samples or performing other operations, it can improve the convenience of operation and reduce the risk of damage to the microplate 3 and cell samples.

[0051] Both the incubator 1 and the door 101 are made of visible glass. By setting up visible glass structures for the incubator 1 and the door 101, operators can easily observe the growth of cells and the effect of drug administration at any time without having to open the door 101. This reduces the interference of the external environment on the internal environment of the incubator 1 and ensures the stability of the cell culture environment. At the same time, the visible design also facilitates real-time recording of experimental phenomena, providing intuitive data references for drug screening experiments.

[0052] Among them, the mounting bracket 2 has a hollow structure. The hollow structure of the mounting bracket can improve the heat conduction of the microplate 3 and help to evenly distribute the temperature of the cell sample inside the microwell 301.

[0053] It is worth noting that the model of the drug delivery pump 505 can be PGP505A, the model of the temperature sensor 601 can be PT100, the model of the nebulizer 8 can be YW-1000, the model of the humidity sensor 801 can be HIH-4000, and the model of the controller 10 can be S7-1200 series PLC.

[0054] The optical imaging system 904 includes a microscope module, an autofocus system, and an image processing module. The fluorescence detection system 905 includes an excitation source, a fluorescence detector, an optical path system, and a signal processing module. Both the optical imaging system 904 and the fluorescence detection system 905 are existing conventional technologies, and their specific structures and principles will not be described in detail here.

[0055] As is well known to those skilled in the art, the working principles and wiring methods of the first hydraulic cylinder 4, the drug delivery pump 505, the first semiconductor cooling chip 6, the temperature sensor 601, the second semiconductor cooling chip 7, the atomizer 8, the humidity sensor 801, the second hydraulic cylinder 9, the third hydraulic cylinder 902, the optical imaging system 904, the fluorescence detection system 905, and the controller 10 are common knowledge and are all conventional methods or common common sense. They will not be described in detail here. Those skilled in the art can arbitrarily select and match their models according to their needs or convenience.

[0056] Working principle:

[0057] Open the box door 101, insert the microporous plate 3 with pull ring 302 into the mounting bracket 2 through the snap-fit ​​groove 201, the rubber pad 202 ensures that the microporous plate is installed firmly, and then connect the fourth delivery pipe 504 to the drug supply system to complete the preparation of the drug dosing component.

[0058] Then, the controller 10 controls the first hydraulic cylinder 4 to descend, driving the first mounting plate 401 and the dosing needle 501 below it to approach the micro-orifice plate 3. The drug delivery pump 505 starts, and delivers the drug in the drug supply system to each dosing needle 501 in a precise and quantitative manner through multiple delivery tubes, completing the drug delivery operation to the corresponding micro-orifice 301. After the drug delivery is completed, the first hydraulic cylinder 4 rises, and the dosing needle 501 is reset.

[0059] The temperature sensor 601 monitors the internal temperature of the incubator 1 in real time. When the temperature deviates from the set value, the controller 10 controls the first semiconductor cooling chip 6 and the second semiconductor cooling chip 7 to work, and adjusts the temperature in both directions to quickly reach and maintain the set temperature.

[0060] Humidity is monitored in real time by humidity sensor 801. When the humidity is lower than the set value, controller 10 starts nebulizer 8 to increase humidity and adjusts it in conjunction with dehumidification measures of condenser plate 701 to ensure constant humidity in incubator.

[0061] The controller 10 controls the second hydraulic cylinder 9 to move the optical imaging system 904 and the fluorescence detection system 905 above the microplate 3. The third hydraulic cylinder 902 adjusts the detection height of the optical imaging system 904 and the fluorescence detection system 905 to monitor the microwells 301 at different positions of the microplate 3. The optical imaging system 904 can observe changes in cell morphology, and the fluorescence detection system 905 can detect the fluorescence signal of the fluorescently labeled cells to comprehensively obtain cell culture information.

[0062] After the experiment, open the chamber door 101 and take out the microplate 3 through the pull ring 302 for further processing. At the same time, clean the condensate in the water collection box 103 and clean and maintain the inside of the incubator to prepare for the next experiment.

[0063] The foregoing description illustrates and describes several preferred embodiments of the present invention. However, as previously stated, it should be understood that the present invention is not limited to the forms disclosed herein and should not be construed as excluding other embodiments. It can be used in various other combinations, modifications, and environments, and can be altered within the scope of the inventive concept described herein through the foregoing teachings or techniques or knowledge in related fields. Any modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention should be within the protection scope of the appended claims.

Claims

1. A high-throughput cell culture device for tumor drug screening, characterized by, include: An incubator (1) has a door (101) that is rotatably installed on one side. A microplate (3) is detachably installed inside the incubator (1). The microplate (3) has a number of microwells (301) for cell culture for tumor drug screening. The dosing component includes a plurality of dosing needles (501), the outlet of each dosing needle (501) being aligned with one of the micropores (301). The temperature control component includes a first semiconductor refrigeration chip (6) and a second semiconductor refrigeration chip (7) fixedly installed on the inner wall of the incubator (1).

2. The high-throughput cell culturing device for tumor drug screening according to claim 1, characterized in that: Also includes: The monitoring component includes an optical imaging system (904) and a fluorescence detection system (905), which are movably mounted above the microplate (3); The controller (10) controls the dosing component, the temperature control component, and the monitoring component.

3. The high-throughput cell culturing device for tumor drug screening according to claim 2, characterized in that: An installation frame (2) is fixedly installed on the inner wall of the incubator (1). The installation frame (2) is provided with a snap-fit ​​groove (201). The microporous plate (3) is fitted and inserted into the snap-fit ​​groove (201). Rubber pads (202) are fixedly installed on both sides of the inner wall of the snap-fit ​​groove (201), and the rubber pads (202) are in contact with the outer side of the microporous plate (3).

4. The high-throughput cell culturing device for tumor drug screening according to claim 3, characterized in that: The dosing component also includes a first hydraulic cylinder (4) fixedly installed on the top of the incubator (1). The telescopic end of the first hydraulic cylinder (4) extends into the interior of the incubator (1) and is fixedly connected to a first mounting plate (401). A plurality of first delivery pipes (5) are fixedly installed at the bottom of the first mounting plate (401). A plurality of dosing needles (501) are respectively connected to a plurality of first delivery pipes (5). One end of the first delivery pipe (5) is fixedly connected to a third delivery pipe (503) through a second delivery pipe (502). One end of a plurality of third delivery pipes (503) is fixedly connected to a fourth delivery pipe (504). The fourth delivery pipe (504) is fixedly installed on the top of the incubator (1), and the upper end of the fourth delivery pipe (504) extends out of the outside of the incubator (1) and is connected to the drug supply system. A drug delivery pump (505) is fixedly installed on the fourth delivery pipe (504). The controller (10) is electrically connected to the first hydraulic cylinder (4) and the drug delivery pump (505).

5. The high-throughput cell culturing device for tumor drug screening according to claim 4, characterized in that: The first semiconductor refrigeration chip (6) and the second semiconductor refrigeration chip (7) are symmetrically installed on both sides of the inner wall of the incubator (1). The hot end of the first semiconductor refrigeration chip (6) faces the inside of the incubator (1) and the cold end faces the outside of the incubator (1). The cold end of the second semiconductor refrigeration chip (7) faces the inside of the incubator (1) and the hot end faces the outside of the incubator (1). A temperature sensor (601) is fixedly installed on the inner wall of the incubator (1), and the controller (10) is electrically connected to the first semiconductor refrigeration chip (6), the second semiconductor refrigeration chip (7) and the temperature sensor (601).

6. The high-throughput cell culturing device for tumor drug screening according to claim 5, characterized in that: A condenser plate (701) is fixedly installed at the cold end of the second semiconductor cooling chip (7). The bottom of the incubator (1) is equipped with a water collection box (103) with an open top, and the condenser plate (701) is located above the opening of the water collection box (103).

7. The high-throughput cell culture device for tumor drug screening according to claim 6, characterized in that: An atomizer (8) and a humidity sensor (801) are fixedly installed on the inner wall of the incubator (1), and the controller (10) is electrically connected to the atomizer (8) and the humidity sensor (801) respectively.

8. The high-throughput cell culturing device for tumor drug screening according to claim 7, characterized in that: The incubator (1) has a mounting cavity (102) on one side, and the monitoring component is placed inside the mounting cavity (102); The monitoring component also includes a second hydraulic cylinder (9) fixedly installed on the outer wall of the mounting cavity (102). The telescopic end of the second hydraulic cylinder (9) extends into the interior of the mounting cavity (102) and is fixedly connected to a connecting plate (901). A third hydraulic cylinder (902) is fixedly connected to one end of the connecting plate (901). A second mounting plate (903) is fixedly connected to the telescopic end of the third hydraulic cylinder (902). The optical imaging system (904) and the fluorescence detection system (905) are fixedly installed at the bottom of the second mounting plate (903). The controller (10) is electrically connected to the second hydraulic cylinder (9), the third hydraulic cylinder (902), the optical imaging system (904), and the fluorescence detection system (905), respectively.

9. The high-throughput cell culturing device for tumor drug screening according to claim 1, characterized in that: A pull ring (302) is fixedly installed at one end of the microporous plate (3).

10. The high-throughput cell culturing device for tumor drug screening according to claim 3, characterized in that: The incubator (1) and the door (101) are both made of visible glass, and the mounting bracket (2) is a hollow structure.

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