A pollution-preventing filter type nucleic acid extraction tool for fruit and vegetable samples

Abstract

The utility model relates to fruit and vegetable technical field discloses a fruit and vegetable sample anti -pollution filter type nucleic acid extraction instrument, including the body, the body one side is provided with replacement mechanism, the body one side is provided with filter mechanism, the body surface is provided with measuring mechanism. This fruit and vegetable sample anti -pollution filter type nucleic acid extraction instrument is provided with replacement mechanism, in the working process of fruit and vegetable sample anti -pollution filter type nucleic acid extraction instrument to fruit and vegetable sample nucleic acid bacteria -proof filter extraction, utilizes the rotary block to drive fixed bad to rotate, utilizes spring a to drive the limiting rod a to enter the inside of limiting slot, utilizes the stirring head to stir fruit and vegetable sample, avoids the working process of fruit and vegetable sample nucleic acid bacteria -proof filter extraction, because fruit and vegetable sample is easy to deposit phenomenon, and then when extracting fruit and vegetable sample, it is easy to extract concentration uneven phenomenon, and then causes the influence to subsequent experimental result, leads to experimental data error.

Description

Technical Field

[0001] This utility model relates to the field of fruit and vegetable technology, specifically to a nucleic acid extraction tool for preventing contamination of fruit and vegetable samples using a filtering method. Background Technology

[0002] Fruits refer to juicy, edible plant fruits with a predominantly sweet and sour taste. Fruits are not only rich in vitamins but also promote digestion. Vegetables refer to plants or fungi that can be cooked and eaten; they are an essential part of people's daily diet. Vegetables provide the human body with various essential vitamins and minerals. However, fruits and vegetables may contain large amounts of pathogenic microorganisms and insect larvae, seriously affecting the quality of these agricultural products. In recent years, the detection of biological factors carried by these fruits and vegetables has mainly relied on nucleic acid molecular characterization technology. The grinding and nucleic acid extraction processes of fruit and vegetable samples are prerequisites for accurate detection. However, the requirements for grinding devices vary depending on the sample materials and experimental scenarios. Currently, the nucleic acid extraction process for biological risk factors in fruits and vegetables requires grinding and adding lysis buffer separately, which is cumbersome and prone to contamination. For fruits and vegetables, there is currently no commercially available equipment that simultaneously meets the requirements of simple operation, contamination prevention, and portability, integrating sample grinding and nucleic acid extraction.

[0003] A search revealed an existing patent (publication number: CN212482976U) that discloses a liquid sampler, including a sampler tank. The tank has an inlet at the bottom and a outlet on the side. An upper sealing plate is installed at the tank opening, and a lower sealing plate is installed at the bottom. When the upper sealing plate is opened and the sampler tank is placed into the liquid to be sampled, the lower sealing plate is hydraulically compressed and rises, allowing the liquid to enter the tank. After closing the upper sealing plate, the sampler tank is lifted, and the lower sealing plate returns to its original position under the weight of the liquid inside, sealing the inlet and completing the sampling. This invention provides a liquid sampler with excellent sampling effect, long service life, stable performance, and high safety and reliability.

[0004] However, existing equipment for grinding fruit and vegetable samples is bulky, requiring multiple devices to prevent cross-contamination, making it inconvenient to carry and handle. After grinding, adding lysis buffer for nucleic acid extraction necessitates changing containers, making the process cumbersome and time-consuming. This two-step extraction process is highly susceptible to contamination, potentially affecting the accuracy of subsequent testing results and leading to false positives.

[0005] Therefore, a nucleic acid extraction tool for preventing contamination of fruit and vegetable samples is proposed to address the above problems. Utility Model Content

[0006] (a) Technical problems to be solved

[0007] To address the shortcomings of existing technologies, this invention provides a contamination-resistant, filtering-type nucleic acid extraction tool for fruit and vegetable samples. This solves the problems mentioned in the background section regarding existing equipment for grinding fruit and vegetable samples, which is bulky and requires multiple devices to prevent cross-contamination, making it inconvenient to carry and handle. Furthermore, the process of adding lysis buffer to the ground sample for nucleic acid extraction necessitates changing containers, making the operation cumbersome and time-consuming. The two-step extraction process is highly susceptible to contamination, which can negatively impact the accuracy of subsequent testing results and lead to false positives.

[0008] (II) Technical Solution

[0009] To achieve the above objectives, this utility model provides the following technical solution: a nucleic acid extraction tool for preventing contamination of fruit and vegetable samples by filtering, comprising a body, a replacement mechanism on one side of the body, a filtering mechanism on one side of the body, and a measuring mechanism on the surface of the body;

[0010] The replacement mechanism includes an opening located on one side of the vessel body. A rotating block is provided on one side of the opening, and a fixing block is provided on one side of the rotating block. A limiting groove a is provided on the surface of the fixing block, and a limiting rod a is provided inside the limiting groove a. A spring a is provided on one side of the limiting rod a, and a support plate a is provided on one side of the spring a.

[0011] As a preferred embodiment, a housing is provided on one side of the support plate a, a motor is provided inside the housing, and a rotating shaft is provided on one side of the motor.

[0012] As a preferred embodiment, a telescopic rod is provided on one side of the rotating shaft, a groove is provided on the surface of the telescopic rod, a slider is provided inside the groove, and a telescopic sleeve is provided on the surface of the slider.

[0013] As a preferred embodiment, a limiting groove b is formed on the surface of the telescopic sleeve, a limiting rod b is provided inside the limiting groove b, a spring b is provided on one side of the limiting rod b, the spring b is provided on the surface of the telescopic rod, and a stirring head is provided on one side of the telescopic sleeve.

[0014] As a preferred embodiment, the filtering mechanism includes a limiting rod c, which is disposed inside the limiting groove a, and a spring c is provided on one side of the limiting rod c, and a support plate c is provided on one side of the spring c.

[0015] As a preferred embodiment, a sleeve is provided on one side of the support plate c, a cylinder head is provided on one side of the sleeve, and a pull-out head is provided on one side of the cylinder head.

[0016] As a preferred embodiment, a sealing ring is provided on the other side of the cylinder head, a filter cylinder is provided on one side of the sealing ring, and a filter cotton sheet is provided on one side of the filter cylinder.

[0017] As a preferred embodiment, the measuring mechanism includes a scale bar disposed on the surface of the instrument body, a movable groove formed on the surface of the scale bar, a movable rod disposed inside the movable groove, the movable rod disposed on the surface of the instrument body, a piston disposed inside the instrument body, and a core rod disposed below the piston.

[0018] This invention provides a contamination-resistant, filtering nucleic acid extraction tool for fruit and vegetable samples. It features the following:

[0019] Beneficial effects:

[0020] (1) The fruit and vegetable sample anti-contamination filtration nucleic acid extraction tool is equipped with a replacement mechanism. During the process of extracting nucleic acid from fruit and vegetable samples using the anti-contamination filtration nucleic acid extraction tool, the rotating block can be rotated first, and the fixed block can be rotated by the rotating block. Then, under the action of the extension and reset of the spring a, the spring a drives the limiting rod a into the inner side of the limiting groove a, which facilitates the replacement and installation of the stirring head. The stirring head is used to stir the fruit and vegetable samples, avoiding the precipitation phenomenon that may occur in fruit and vegetable samples during the anti-contamination filtration nucleic acid extraction process. This can lead to uneven extraction concentration, which in turn affects the subsequent experimental results and causes errors in the experimental data.

[0021] (2) The fruit and vegetable sample anti-contamination filtration nucleic acid extraction tool is equipped with a replacement mechanism. During the process of extracting nucleic acid from fruit and vegetable samples using the fruit and vegetable sample anti-contamination filtration nucleic acid extraction tool, the motor switch can be turned on first. Under the action of the motor, the rotating shaft is driven to rotate, and the rotating shaft drives the stirring head to rotate, thereby stirring the fruit and vegetable samples so that the concentration of each part of the fruit and vegetable samples is the same. Under the action of the telescopic rod and the telescopic sleeve, the length of the stirring head can be adjusted according to the depth of the fruit and vegetable samples. At the same time, under the action of the spring b, the limiting rod b is driven to enter the inner side of the limiting groove b to fix the position of the stirring head. Attached Figure Description

[0022] Figure 1 This is a side view of the appearance structure of this utility model;

[0023] Figure 2 This is a schematic diagram of the replacement mechanism of this utility model;

[0024] Figure 3 This is a schematic diagram of the filter mechanism structure of this utility model;

[0025] Figure 4 This is a schematic diagram of the measuring mechanism structure of this utility model;

[0026] Figure 5 for Figure 2 Enlarged structural diagram at point A in the middle.

[0027] The components include: 1. Body; 2. Replacement mechanism; 201. Mouth opening; 202. Rotating block; 203. Fixing block; 204. Limiting groove a; 205. Limiting rod a; 206. Spring a; 207. Support plate a; 2071. Outer shell; 2072. Motor; 2073. Rotating shaft; 2074. Telescopic rod; 20741. Slide groove; 20742. Sliding block; 20743. Telescopic sleeve; 20744. Limiting groove b; 20745. 1. Limiting rod b; 20746. Spring b; 2075. Stirring head; 3. Filtering mechanism; 301. Limiting rod c; 302. Spring c; 303. Support plate c; 304. Sleeve; 305. Cylinder head; 306. Extraction head; 307. Sealing ring; 308. Filter cylinder; 309. Filter cotton sheet; 4. Measuring mechanism; 401. Scale bar; 402. Movable groove; 403. Movable rod; 404. Piston; 405. Core rod. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] Please see Figures 1 to 5 This utility model provides a technical solution: a nucleic acid extraction tool for preventing contamination of fruit and vegetable samples by filtering, including a body 1, a replacement mechanism 2 on one side of the body 1, a filtering mechanism 3 on one side of the body 1, a measuring mechanism 4 on the surface of the body 1, the measuring mechanism 4 including a scale bar 401, the scale bar 401 being disposed on the surface of the body 1, a movable groove 402 being formed on the surface of the scale bar 401, a movable rod 403 being disposed inside the movable groove 402, the movable rod 403 being disposed on the surface of the body 1, a piston 404 being disposed inside the body 1, and a core rod 405 being disposed below the piston 404.

[0030] In the process of extracting nucleic acid from fruit and vegetable samples using a pollution-proof filtration-type nucleic acid extraction tool, the inclusion of a replacement mechanism 2 can prevent precipitation and uneven extraction concentrations caused by the natural precipitation of fruit and vegetable samples during the extraction process. This, in turn, can affect subsequent experimental results and lead to errors in the experimental data.

[0031] Please see Figure 2A nucleic acid extraction tool for preventing contamination of fruit and vegetable samples using a filtering method, comprising a replacement mechanism 2 including an inlet 201, the inlet 201 being located on one side of the body 1, a rotating block 202 being provided on one side of the inlet 201, a fixing block 203 being provided on one side of the rotating block 202, a limiting groove a204 being provided on the surface of the fixing block 203, a limiting rod a205 being provided inside the limiting groove a204, a spring a206 being provided on one side of the limiting rod a205, a support plate a207 being provided on one side of the spring a206, a housing 2071 being provided on one side of the support plate a207, and a motor 2072 being provided inside the housing 2071. A rotating shaft 2073 is provided on one side, and a telescopic rod 2074 is provided on one side of the rotating shaft 2073. A groove 20741 is provided on the surface of the telescopic rod 2074. A slider 20742 is provided inside the groove 20741. A telescopic sleeve 20743 is provided on the surface of the slider 20742. A limiting groove b20744 is provided on the surface of the telescopic sleeve 20743. A limiting rod b20745 is provided inside the limiting groove b20744. A spring b20746 is provided on one side of the limiting rod b20745. The spring b20746 is provided on the surface of the telescopic rod 2074. A stirring head 2075 is provided on one side of the telescopic sleeve 20743.

[0032] In the process of extracting nucleic acid from fruit and vegetable samples using a pollution-proof filtration-type nucleic acid extraction tool, the rotating block 202 can be rotated first, which in turn drives the fixed block 203 to rotate. Then, under the extension and resetting action of the spring a206, the spring a206 drives the limiting rod a205 into the limiting groove a204, facilitating the replacement and installation of the stirring head 2075. The stirring head 2075 is then used to stir the fruit and vegetable samples, preventing precipitation and uneven extraction concentrations that can occur during the nucleic acid extraction process. To prevent errors in experimental data and subsequent experimental results, the motor 2072 can be turned on first. Under the action of the motor 2072, the rotating shaft 2073 is rotated, which in turn rotates the stirring head 2075, thus stirring the fruit and vegetable samples to ensure that the concentration of each part of the samples is the same. Under the action of the telescopic rod 2074 and the telescopic sleeve 20743, the length of the stirring head 2075 can be adjusted according to the depth of the fruit and vegetable samples. At the same time, under the action of the spring b20746, the limiting rod b20745 is driven into the limiting groove b20744 to fix the position of the stirring head 2075.

[0033] Please see Figure 3A nucleic acid extraction tool for preventing contamination of fruit and vegetable samples using a filtering mechanism 3 includes a limiting rod c301, which is located inside a limiting groove a204. A spring c302 is provided on one side of the limiting rod c301, a support plate c303 is provided on one side of the spring c302, a sleeve 304 is provided on one side of the support plate c303, a cylinder head 305 is provided on one side of the sleeve 304, an extraction head 306 is provided on one side of the cylinder head 305, a sealing ring 307 is provided on the other side of the cylinder head 305, a filter cylinder 308 is provided on one side of the sealing ring 307, and a filter cotton sheet 309 is provided on one side of the filter cylinder 308.

[0034] Among them: In the process of extracting nucleic acid from fruit and vegetable samples by anti-contamination filtration, the filter mechanism 3 is set up, and the filter cotton 309 can be used to filter and sample the fruit and vegetable samples.

[0035] The working principle of this utility model is as follows: In the process of nucleic acid extraction from fruit and vegetable samples using the anti-contamination filtering nucleic acid extraction tool, the rotating block 202 can be rotated first, which drives the fixed block 203 to rotate. Then, under the action of the extension and resetting action of the spring a206, the spring a206 drives the limiting rod a205 into the inner side of the limiting groove a204, which facilitates the replacement and installation of the stirring head 2075. The stirring head 2075 is used to stir the fruit and vegetable samples, avoiding the precipitation phenomenon that easily occurs in fruit and vegetable samples during the nucleic acid anti-contamination filtering extraction process. This can easily lead to uneven extraction concentration, which in turn affects the subsequent experimental results. If data errors occur, the motor 2072 can be turned on first. Under the action of the motor 2072, the rotating shaft 2073 is rotated, and the rotating shaft 2073 drives the stirring head 2075 to rotate, thereby stirring the fruit and vegetable samples to make the concentration of each part of the fruit and vegetable samples the same. Under the action of the telescopic rod 2074 and the telescopic sleeve 20743, the length of the stirring head 2075 can be adjusted according to the depth of the fruit and vegetable samples. At the same time, under the action of the spring b20746, the limiting rod b20745 is driven into the inner side of the limiting groove b20744 to fix the position of the stirring head 2075. Secondly, through the filter mechanism 3, the filter cotton sheet 309 can be used to filter and sample the fruit and vegetable samples.

[0036] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid extraction tool for preventing contamination of fruit and vegetable samples using a filtering method, comprising a body (1), characterized in that: A replacement mechanism (2) is provided on one side of the device (1), a filtering mechanism (3) is provided on one side of the device (1), and a measuring mechanism (4) is provided on the surface of the device (1). The replacement mechanism (2) includes an opening (201), which is located on one side of the body (1). A rotating block (202) is provided on one side of the opening (201), and a fixing block (203) is provided on one side of the rotating block (202). A limiting groove a (204) is provided on the surface of the fixing block (203), and a limiting rod a (205) is provided inside the limiting groove a (204). A spring a (206) is provided on one side of the limiting rod a (205), and a support plate a (207) is provided on one side of the spring a (206).

2. The fruit and vegetable sample anti-contamination filtering nucleic acid extraction tool according to claim 1, characterized in that: A housing (2071) is provided on one side of the support plate a (207), a motor (2072) is provided inside the housing (2071), and a rotating shaft (2073) is provided on one side of the motor (2072).

3. The fruit and vegetable sample anti-contamination filtering nucleic acid extraction tool according to claim 2, characterized in that: A telescopic rod (2074) is provided on one side of the rotating shaft (2073). A groove (20741) is provided on the surface of the telescopic rod (2074). A slider (20742) is provided inside the groove (20741). A telescopic sleeve (20743) is provided on the surface of the slider (20742).

4. The fruit and vegetable sample anti-contamination filtering nucleic acid extraction tool according to claim 3, characterized in that: The telescopic sleeve (20743) has a limiting groove b (20744) on its surface. A limiting rod b (20745) is provided inside the limiting groove b (20744). A spring b (20746) is provided on one side of the limiting rod b (20745). The spring b (20746) is provided on the surface of the telescopic rod (2074). A stirring head (2075) is provided on one side of the telescopic sleeve (20743).

5. The fruit and vegetable sample anti-contamination filtering nucleic acid extraction tool according to claim 1, characterized in that: The filter mechanism (3) includes a limiting rod c (301), which is disposed inside the limiting groove a (204). A spring c (302) is disposed on one side of the limiting rod c (301), and a support plate c (303) is disposed on one side of the spring c (302).

6. The fruit and vegetable sample anti-contamination filtering nucleic acid extraction tool according to claim 5, characterized in that: A sleeve (304) is provided on one side of the support plate c (303), a cylinder head (305) is provided on one side of the sleeve (304), and a pull-out head (306) is provided on one side of the cylinder head (305).

7. The fruit and vegetable sample anti-contamination filtering nucleic acid extraction tool according to claim 6, characterized in that: A sealing ring (307) is provided on the other side of the cylinder head (305), a filter cylinder (308) is provided on one side of the sealing ring (307), and a filter cotton sheet (309) is provided on one side of the filter cylinder (308).

8. The fruit and vegetable sample anti-contamination filtering nucleic acid extraction tool according to claim 1, characterized in that: The measuring mechanism (4) includes a scale bar (401) which is disposed on the surface of the body (1). A movable groove (402) is provided on the surface of the scale bar (401). A movable rod (403) is provided inside the movable groove (402). The movable rod (403) is disposed on the surface of the body (1). A piston (404) is provided inside the body (1). A core rod (405) is provided below the piston (404).

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