An electrode structure and electroporation electrode assembly

Abstract

This utility model relates to an electrode structure and an electroporation electrode assembly. The electrode structure includes: an insulating base with a first side and a second side disposed opposite to each other; a first heat-conducting component mounted on the first side of the insulating base; a second heat-conducting component mounted on the second side of the insulating base; the second heat-conducting component and the first heat-conducting component are disposed opposite to each other and form a clamping space for clamping an electroporation cup; a temperature-changing component including a positive temperature-changing end connected to the first heat-conducting component and the second heat-conducting component; and a first temperature detection component mounted on the first heat-conducting component and / or the second heat-conducting component at a position opposite to the clamping space for detecting the load end temperature of the electroporation cup. This utility model ensures the safety of the electrode structure after it is energized and can ensure that the load end temperature is kept constant while providing the electrical connection required for electroporation. It has a simple structure, low cost, and is easy to operate. It can clamp different types of electroporation cups and is easy to standardize.

Description

Technical Field

[0001] This utility model relates to the field of electrode technology, and in particular to an electrode structure and an electroporation electrode assembly. Background Technology

[0002] With the rapid development of medical technology, electroporation technology has become widely used and is an important method for cell electrotransfection. An electroporation cup is a device for achieving cell electrotransfection. It includes a cup body with an internal space and positive and negative electrodes mounted on two relatively parallel sides of the cup body. One side of each electrode is located within the internal space, while the other side is exposed to the external environment. After the positive and negative electrodes are connected to the external environment and energized, electrotransfection of the cell fluid within the internal space can be achieved. In existing technologies, electrode structure design presents some technical challenges in application. For example, it is difficult to guarantee the timeliness and stability of temperature control during cell electrotransfection, and the electrode structure design scheme is costly. Furthermore, the ease of operation and standardization need to be improved. Summary of the Invention

[0003] This utility model provides an electrode structure and an electroporation electrode assembly to solve the technical problems in the prior art, such as the inability of electrode structures to guarantee the timeliness and stability of temperature control of the electroporation cup during cell electroporation.

[0004] An embodiment of this utility model provides an electrode structure, comprising:

[0005] An insulating base, comprising a first side and a second side disposed opposite to each other;

[0006] A first heat-conducting component is installed on the first side of the insulating base;

[0007] The second heat-conducting component is installed on the second side of the insulating base; the second heat-conducting component and the first heat-conducting component are arranged opposite to each other and form a clamping space for clamping the electric rotary cup;

[0008] A temperature-changing component, including a positive temperature-changing end connected to the first heat-conducting component and the second heat-conducting component;

[0009] A first temperature sensing element is installed on the first heat-conducting component and / or the second heat-conducting component at a position opposite to the clamping space, for detecting the load end temperature of the electric rotary cup.

[0010] Optionally, the temperature-changing element further includes a reverse temperature-changing end, and the electrode structure further includes a heat sink connected to the reverse temperature-changing end.

[0011] Optionally, the electrode structure further includes a limit switch mounted on the insulating base and extending into the clamping space.

[0012] Optionally, the first heat-conducting component includes a first heat-conducting block mounted on the first side and connected to the positive temperature-changing end, and a first elastic heat-conducting element disposed on the first heat-conducting block;

[0013] The second heat-conducting component includes a second heat-conducting block mounted on the second side and connected to the positive temperature-changing end, and a second elastic heat-conducting element disposed on the first heat-conducting block;

[0014] The first elastic heat-conducting element and the second elastic heat-conducting element are arranged opposite to each other to form the clamping space.

[0015] Optionally, the first heat-conducting block is provided with a first sliding groove, and the first elastic heat-conducting element includes a first elastic element and a third heat-conducting block; the third heat-conducting block is slidably installed in the first sliding groove through the first elastic element;

[0016] The second heat-conducting block is provided with a second sliding groove, and the second elastic heat-conducting element includes a second elastic element and a fourth heat-conducting block; the fourth heat-conducting block is slidably installed in the second sliding groove through the second elastic element.

[0017] Optionally, the first groove is provided with a first positioning post, the end face of the third heat-conducting block facing away from the fourth heat-conducting block is recessed to form a first groove, and a second positioning post is provided in the first groove at a position opposite to the first positioning post; the first elastic element is a first spring, and the opposite ends of the first spring are respectively sleeved in the first positioning post and the second positioning post;

[0018] The second groove is provided with a third positioning post, and the end face of the fourth heat-conducting block opposite to the third heat-conducting block is recessed to form a second groove. The fourth positioning post is provided in the second groove at a position opposite to the third positioning post. The second elastic element is a second spring, and the opposite ends of the second spring are respectively sleeved in the third positioning post and the fourth positioning post.

[0019] Optionally, the first elastic heat-conducting member has a first guide slope and a first clamping surface connected to the first guide slope on its end face relative to the second elastic heat-conducting member, and the first clamping surface has at least one first rib; the second elastic heat-conducting member has a second guide slope and a second clamping surface connected to the second guide slope on its end face relative to the first elastic heat-conducting member, and the second clamping surface has at least one second rib; and / or

[0020] The first elastic heat-conducting component has a first limiting groove on its end face facing the second elastic heat-conducting component, and the second elastic heat-conducting component has a second limiting groove on its end face facing the first elastic heat-conducting component; the first temperature detection component includes a first temperature probe installed in the first limiting groove and a second temperature probe installed in the second limiting groove.

[0021] Optionally, the temperature-changing component includes a first cooling element that is bonded to the first heat-conducting component and a second cooling element that is bonded to the second heat-conducting component;

[0022] The electrode structure further includes a third temperature probe mounted on the first heat-conducting component for detecting the temperature at the positive temperature-changing end of the first cooling chip, and a fourth temperature probe mounted on the second heat-conducting component for detecting the temperature at the positive temperature-changing end of the second cooling chip.

[0023] Optionally, the electrode structure further includes a second temperature sensing element mounted on the first heat-conducting component and / or the second heat-conducting component and used to detect the temperature of the positive temperature-changing end.

[0024] Optionally, the temperature-changing component includes a first cooling element that is bonded to the first heat-conducting component and a second cooling element that is bonded to the second heat-conducting component;

[0025] The second temperature detection device includes a third temperature probe mounted on the first heat-conducting assembly and used to detect the temperature at the positive temperature-changing end of the first cooling chip, and a fourth temperature probe mounted on the second heat-conducting assembly and used to detect the temperature at the positive temperature-changing end of the second cooling chip.

[0026] Optionally, the electrode structure further includes an insulating shell, an insulating inner liner, and an insulating backing. The first and second thermally conductive components are mounted on the insulating base via the insulating inner liner. The insulating backing is mounted on the side of the insulating base away from the clamping space, and the insulating shell covers the surfaces of the first and second thermally conductive components.

[0027] Optionally, the insulating backing is provided with a first through hole and a second through hole, and the electrode structure further includes a first plug that passes through the first through hole and connects to the first heat-conducting component, and a second plug that passes through the second through hole and connects to the second heat-conducting component.

[0028] An electroporation electrode assembly includes an electroporation cup and the aforementioned electrode structure.

[0029] In this invention, the first and second heat-conducting components of the electrode structure form the two poles of a conductive electrode after being energized. The first and second heat-conducting components are insulated from each other by an insulating base, ensuring the safety of the electrode structure after it is energized. Furthermore, the clamping space between the first and second heat-conducting components is used to clamp the electroporation cup. After being clamped in the clamping space, the electroporation cup can be connected to the two poles of the conductive electrode formed by the electrode structure, respectively, to achieve conductivity and electrical pulse input to the positive and negative plates of the electroporation cup. This enables electroporation of the cells within the electroporation cup, providing the electrical connection required for electroporation. When the temperature-changing component starts its cooling operation, the positive temperature-changing end of the component, connected to the first and second heat-conducting components, begins cooling. The cooling capacity is transferred from the positive temperature-changing end through the first and second heat-conducting components to the positive and negative plates on both sides of the electroporation cup, thereby cooling the electroporation cup and the cell fluid inside. Furthermore, if the temperature of the cell fluid in the electroporation cup is too low, the temperature-changing component can heat the electroporation cup and the cell fluid until the cell fluid is heated to a certain temperature range before electroporation, improving the electroporation effect and the cell survival rate in the cell fluid. Simultaneously, a first temperature detection element installed on the first and / or second heat-conducting components is positioned opposite to the clamping space and is used to detect the load end temperature of the electroporation cup in real time. This allows the temperature-changing component's operating state to be controlled based on the measured load end temperature, maintaining a constant load end temperature. The electrode structure described above can control the load end temperature of the electric rotary cup in a timely and stable manner, ensuring that the load end temperature is in a constant temperature state. It can also provide the electrical connection required for electric rotation while maintaining a constant temperature. Furthermore, it has a simple structure, low cost, is easy to operate, and is easy to standardize. Attached Figure Description

[0030] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments of this utility model will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0031] Figure 1 This is a cross-sectional view of an electrode structure for holding an electric rotating cup according to an embodiment of the present invention;

[0032] Figure 2 This is a schematic diagram of the assembly structure of the electrode structure clamping the electric rotary cup according to an embodiment of the present invention;

[0033] Figure 3 This is an exploded view of the electrode structure and the electric rotating cup provided in one embodiment of the present invention.

[0034] The reference numerals in the accompanying drawings are as follows:

[0035] 1. Insulating base; 2. First heat-conducting component; 21. First heat-conducting block; 211. First sliding groove; 22. First elastic heat-conducting element; 221. First elastic element; 222. Third heat-conducting block; 223. First limiting groove; 3. Second heat-conducting component; 31. Second heat-conducting block; 311. Second sliding groove; 32. Second elastic heat-conducting element; 321. Second elastic element; 322. Fourth heat-conducting block; 323. Second limiting groove; 4. Clamping space; 5. Temperature-changing component; 51. The first... 52. Second cooling element; 53. Forward temperature change end; 54. Reverse temperature change end; 6. First temperature detection element; 61. First temperature probe; 62. Second temperature probe; 7. Heat sink; 8. Limit switch; 91. Third temperature probe; 92. Fourth temperature probe; 10. Electric rotating cup; 11. Insulating outer shell; 12. Insulating inner liner; 13. Insulating backing; 131. First through hole; 132. Second through hole; 14. First plug; 15. Second plug. Detailed Implementation

[0036] To make the technical problems solved, technical solutions, and beneficial effects of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0037] like Figures 1 to 3 As shown, an embodiment of the present invention provides an electrode structure comprising:

[0038] The insulating base 1 includes a first side and a second side disposed opposite to each other; wherein the insulating base 1 is made of insulating material.

[0039] The first heat-conducting component 2 is installed on the first side of the insulating base 1. The first heat-conducting component 2 can be a one-piece molded structure or it can be assembled from multiple components.

[0040] The second heat-conducting component 3 is installed on the second side of the insulating base 1; the second heat-conducting component 3 and the first heat-conducting component 2 are arranged opposite to each other and form a clamping space 4 for clamping the electric rotary cup 10; the second heat-conducting component 3 can be a one-piece structure or it can be assembled from multiple components. The installation and connection method between the first heat-conducting component 2 and the second heat-conducting component 3 and the insulating base 1 can be set according to requirements, for example, it can be as follows: Figure 1 As shown, with the insulating base 1 as the center, the first heat-conducting component 2 and the second heat-conducting component 3 are symmetrically assembled on the first and second sides of the insulating base 1. Understandably, the electrode structure may also include an adjustment component for adjusting the size of the clamping space 4 to achieve clamping of electric rotary cups 10 of different sizes.

[0041] The temperature-changing component 5 includes a positive temperature-changing end 53 connected to the first heat-conducting component 2 and the second heat-conducting component 3; wherein, the positive temperature-changing end 53 of the temperature-changing component 5 can be attached with a heat-conducting pad material before being attached to the first heat-conducting component 2 and the second heat-conducting component 3; the temperature-changing component 5 can be designed as a plate or other shapes.

[0042] The first temperature sensing element 6 is installed on the first heat-conducting component 2 and / or the second heat-conducting component 3 at a position opposite to the clamping space 4, for detecting the load end temperature of the electric rotary cup 10. The temperature measuring point of the first temperature sensing element 6 needs to be close to the surface of the electric rotary cup 10 to facilitate the measurement of the load end temperature (surface temperature) of the electric rotary cup 10.

[0043] In this embodiment, the first heat-conducting component 2 and the second heat-conducting component 3 each serve as a single electrode. After being energized, they form the two poles of a conductive electrode. They are respectively installed on the first and second sides of the insulating base 1, ensuring insulation between the two poles of the conductive electrode. Simultaneously, the gap between the second heat-conducting component 3 and the first heat-conducting component 2, forming the clamping space 4 for holding the electric rotating cup 10, is maintained. In the above embodiment, the first temperature detection element 6 continuously measures the load end temperature of the electric rotating cup 10 and sends it to the control system. The control system can then adjust the cooling power (or heating power) of the temperature-changing element 5 based on the load end temperature feedback from the first temperature detection element 6, thereby controlling the working state of the temperature-changing element 5 and maintaining the load end temperature at a constant temperature, achieving timely and stable temperature control. This invention, through stable clamping and constant temperature control of the electric rotating cup 10, can achieve high-throughput continuous electric rotation of electric rotating cups 10 of different volumes. Furthermore, integrating the first temperature detection element 6 into the electrode structure can reduce the cost of consumables such as the electric rotating cup 10, thereby reducing overall cost.

[0044] In this embodiment, the electrode structure can be designed as a horizontal structure, a vertical structure, or an embedded structure, with the electric rotating cup 10 placed inside the electrode structure.

[0045] In the above embodiments of this utility model, the first heat-conducting component 2 and the second heat-conducting component 3 of the electrode structure form the two poles of a conductive electrode after being energized. The first heat-conducting component 2 and the second heat-conducting component 3 are insulated from each other by an insulating base 1, ensuring the safety of the electrode structure after it is energized. Furthermore, the clamping space 4 between the first heat-conducting component 2 and the second heat-conducting component 3 is used to clamp the electro-rotating cup 10. After the electro-rotating cup 10 is clamped in the clamping space 4, it can be connected to the two poles of the conductive electrode formed by the electrode structure, thereby enabling the conduction of electricity and the input of electrical pulses to the positive and negative plates of the electro-rotating cup 10. This allows for electroporation of the cells within the cup of the electro-rotating cup 10, providing the electrical connection required for electro-rotation. When the temperature-changing component 5 starts cooling, the positive temperature-changing end 53 of the temperature-changing component 5, which is connected to the first heat-conducting component 2 and the second heat-conducting component 3, begins cooling. The cooling capacity is transferred from the positive temperature-changing end 53 through the first heat-conducting component 2 and the second heat-conducting component 3 to the positive and negative electrode plates on both sides of the electroporation cup 10, thereby cooling the electroporation cup 10 and the cell fluid inside the cup. Furthermore, when the temperature of the cell fluid in the electroporation cup 10 is too low, the temperature-changing component 5 can also heat the electroporation cup 10 and the cell fluid inside the cup until the cell fluid in the electroporation cup 10 is heated to a certain temperature range before electroporation, thereby improving the electroporation effect and the cell survival rate in the cell fluid. At the same time, the first temperature detection component 6, installed on the first heat-conducting component 2 and / or the second heat-conducting component 3, is positioned opposite to the clamping space 4 and is used to detect the load end temperature of the electroporation cup 10 in real time. This allows the operating state of the temperature-changing component 5 to be controlled according to the measured load end temperature, so as to keep the load end temperature in a constant state. The electrode structure described above can control the load end temperature of the electric rotary cup 10 in a timely and stable manner, ensuring that the load end temperature is in a constant temperature state. It can also provide the electrical connection required for electric rotation while maintaining a constant temperature. Moreover, its structure is simple, its cost is low, its operation is convenient, and it is easy to standardize.

[0046] In one embodiment, such as Figures 1 to 3 As shown, the temperature-changing component 5 also includes a reverse temperature-changing end 54, and the electrode structure also includes a heat sink 7 connected to the reverse temperature-changing end 54. The reverse temperature-changing end 54 of the temperature-changing component 5 can be attached with a thermally conductive pad material before being attached to the heat sink 7 to facilitate heat dissipation through the heat sink 7. The heat sink 7 has multiple mounting holes spaced apart, and the heat sink 7 is fixed to the insulating base 1 through these mounting holes, allowing the heat sink 7 to be securely mounted on the insulating base 1. Furthermore, the temperature-changing component 5 is tightly attached between the first thermally conductive component 2 (or the second thermally conductive component 3) and the heat sink 7, simultaneously achieving cooling (or heating) at the forward temperature-changing end 53 and heat dissipation at the reverse temperature-changing end 54.

[0047] In one embodiment, such as Figure 1 and Figure 2As shown, the electrode structure also includes a limit switch 8 mounted on the insulating base 1 and extending into the clamping space 4. When the electric rotary cup 10 is inserted into the clamping space 4 and reaches the preset insertion position, it contacts the limit switch 8. The limit switch 8 provides a mechanical sound to indicate to the user that the electric rotary cup 10 has been inserted correctly. Thus, the limit protection and audible prompts provided by the limit switch 8 make the operation of the electric rotary cup 10 more convenient and standardized. Furthermore, the limit switch 8 not only provides audible feedback for the insertion and removal of the electric rotary cup 10 during use, but also provides corresponding installation prompts to the user and sends insertion and removal signals to the control system for subsequent control.

[0048] In one embodiment, such as Figure 1 and Figure 2 As shown, the first heat-conducting component 2 includes a first heat-conducting block 21 mounted on the first side and connected to the positive temperature-changing end 53, and a first elastic heat-conducting element 22 disposed on the first heat-conducting block 21; the second heat-conducting component 3 includes a second heat-conducting block 31 mounted on the second side and connected to the positive temperature-changing end 53, and a second elastic heat-conducting element 32 disposed on the first heat-conducting block 21; the first elastic heat-conducting element 22 and the second elastic heat-conducting element 32 are disposed opposite to each other and form the clamping space 4. In this embodiment, the first elastic heat-conducting element 22 is installed on the end face of the first heat-conducting block 21 facing the second heat-conducting block 31, and the second elastic heat-conducting element 32 is installed on the end face of the second heat-conducting block 31 facing the first heat-conducting block 21. Thus, a clamping space 4 is formed between the first elastic heat-conducting element 22 and the second elastic heat-conducting element 32, and the electroporation cup 10 is clamped between the first elastic heat-conducting element 22 and the second elastic heat-conducting element 32. Since both the first elastic heat-conducting element 22 and the second elastic heat-conducting element 32 are elastic, it is possible to clamp electroporation cups 10 with different thicknesses and gaps, and to be compatible with various electroporation carriers (such as flow, semi-flow, and irregularly shaped electroporation cups), thereby improving the versatility of the electrode structure.

[0049] In one embodiment, such as Figure 1 As shown, the first heat-conducting block 21 is provided with a first sliding groove 211, and the first elastic heat-conducting element 22 includes a first elastic element 221 and a third heat-conducting block 222; the third heat-conducting block 222 is slidably installed in the first sliding groove 211 through the first elastic element 221; the second heat-conducting block 31 is provided with a second sliding groove 311, and the second elastic heat-conducting element 32 includes a second elastic element 321 and a fourth heat-conducting block 322; the fourth heat-conducting block 322 is slidably installed in the second sliding groove 311 through the second elastic element 321. This embodiment can achieve clamping of electroporation cups 10 with different thicknesses and gaps to be compatible with various electroporation carriers (such as flow, semi-flow, and irregularly shaped electroporation cups).

[0050] Furthermore, a first positioning post is provided in the first groove 211, and the end face of the third heat-conducting block 222 facing away from the fourth heat-conducting block 322 is recessed to form a first groove. A second positioning post is provided in the first groove at a position opposite to the first positioning post. The first elastic element 221 is a first spring, and the opposite ends of the first spring are respectively sleeved in the first positioning post and the second positioning post. Understandably, when the electric rotating cup 10 enters the clamping space 4, the end face of the third heat-conducting block 222 facing the fourth heat-conducting block 322 is squeezed. The third heat-conducting block 222 compresses the first spring in the first slide groove 211, causing the third heat-conducting block 222 to move towards the bottom surface of the first slide groove 211, thereby increasing the clamping space 4 to clamp the electric rotating cup 10. Conversely, when the electric rotating cup 10 is removed from the clamping space 4, the end face of the third heat-conducting block 222 facing the fourth heat-conducting block 322 is no longer squeezed, the first spring is no longer squeezed and extends, pushing the third heat-conducting block 222 towards the fourth heat-conducting block 322, thereby reducing the clamping space 4. The second groove 311 is provided with a third positioning post, and the end face of the fourth heat-conducting block 322 facing away from the third heat-conducting block 222 is recessed to form a second groove. The fourth positioning post is provided in the second groove at a position opposite to the third positioning post. The second elastic element 321 is a second spring, and the opposite ends of the second spring are respectively sleeved in the third positioning post and the fourth positioning post. When the electric rotating cup 10 enters the clamping space 4, the end face of the fourth heat-conducting block 322 facing the third heat-conducting block 222 is squeezed. The fourth heat-conducting block 322 squeezes the second spring in the second groove 311 to compress it, so that the fourth heat-conducting block 322 moves towards the bottom surface of the second groove 311, thereby increasing the clamping space 4 to clamp the electric rotating cup 10. Conversely, when the electric rotating cup 10 is taken out of the clamping space 4, the end face of the fourth heat-conducting block 322 facing the third heat-conducting block 222 is no longer squeezed, the second spring is no longer squeezed and extends, pushing the fourth heat-conducting block 322 towards the third heat-conducting block 222, thereby reducing the clamping space 4. This embodiment can clamp electroporation cups 10 with different thicknesses and gaps to be compatible with various electroporation carriers.

[0051] In one embodiment, the first elastic heat-conducting member 22 has a first guide slope and a first clamping surface connected to the first guide slope on its end face relative to the second elastic heat-conducting member 32. The first clamping surface has at least one first rib. The second elastic heat-conducting member 32 has a second guide slope and a second clamping surface connected to the second guide slope on its end face relative to the first elastic heat-conducting member 22. The second clamping surface has at least one second rib. In this embodiment, a large funnel-shaped opening is formed between the first guide slope and the second guide slope, which can guide the electric rotating cup 10 entering the clamping space 4, making it easier for the electric rotating cup 10 to be inserted into the clamping space 4. The first clamping surface and the second clamping surface are set as two parallel planes to ensure flatness, thereby more stably clamping the electric rotating cup 10 entering the clamping space 4. The first rib and the second rib provide clamping friction for the clamped electric rotating cup 10, further ensuring that the electric rotating cup 10 is stably clamped in the clamping space 4. Understandably, the shape and position of the first and second ribs can be set according to requirements and are not limited here. For example, the first rib can be a horizontal stripe located in the middle of the first clamping surface; the second rib can be a horizontal stripe located in the middle of the second clamping surface.

[0052] In one embodiment, such as Figure 1 As shown, the first elastic heat-conducting element 22 has a first limiting groove 223 on its end face facing the second elastic heat-conducting element 32, and the second elastic heat-conducting element 32 has a second limiting groove 323 on its end face facing the first elastic heat-conducting element 22. The first temperature detection element 6 includes a first temperature probe 61 installed in the first limiting groove 223 and a second temperature probe 62 installed in the second limiting groove 323. The first temperature probe 61 and the second temperature probe 62 are bendable, and their ends with temperature measuring points are respectively installed in the first limiting groove 223 and the second limiting groove 323 close to the surface of the electric rotating cup 10, allowing for better measurement of the surface temperature of the electric rotating cup 10.

[0053] In one embodiment, such as Figure 1 and Figure 2As shown, the temperature-changing component 5 includes a first cooling element 51 that is bonded to the first heat-conducting component 2, and a second cooling element 52 that is bonded to the second heat-conducting component 3. The electrode structure further includes a third temperature probe 91 mounted on the first heat-conducting component 2 for detecting the temperature of the positive temperature-changing end 53 of the first cooling element 51, and a fourth temperature probe 92 mounted on the second heat-conducting component 3 for detecting the temperature of the positive temperature-changing end 53 of the second cooling element 52. That is, in this embodiment, the ends of the third temperature probe 91 and the fourth temperature probe 92 with temperature measuring points are respectively mounted close to the surfaces of the positive temperature-changing ends 53 of the first cooling element 51 and the second cooling element 52, so as to better measure the temperature of the positive temperature-changing ends 53 of the first cooling element 51 and the second cooling element 52, and then feed the measured temperature of the positive temperature-changing end 53 back to the control system. Meanwhile, since the first temperature sensing element 6 is close to the electric rotating cup 10 and measures the load end temperature of the electric rotating cup 10, feeding it back to the control system, this invention can simultaneously control the power and start / stop operation of the temperature-changing element 5 based on the temperature of the forward temperature-changing end 53 and the load end temperature, so as to keep the load end temperature in a constant temperature state, thereby forming a constant temperature control in the electrode structure and ensuring the timeliness and stability of temperature control. Understandably, in this invention, the third temperature probe 91 and the fourth temperature probe 92 can be designed as one or more.

[0054] In one embodiment, such as Figure 1 and Figure 2 As shown, the electrode structure further includes an insulating outer shell 11, an insulating inner liner 12, and an insulating backing 13. The first heat-conducting component 2 and the second heat-conducting component 3 are mounted on the insulating base 1 via the insulating inner liner 12. The insulating backing 13 is mounted on the side of the insulating base 1 facing away from the clamping space 4. The insulating outer shell 11 covers the surfaces of the first heat-conducting component 2 and the second heat-conducting component 3. In this embodiment, the assembly structure between the insulating outer shell 11, the insulating inner liner 12, and the insulating backing 13 provides insulation protection for the conductive and heat-conducting units in the electrode structure. That is, the first heat-conducting component 2 and the second heat-conducting component 3 are enclosed by the insulating outer shell 11, the insulating inner liner 12, and the insulating backing 13, ensuring safety during energized use. Two insulating outer shells 11 and two insulating inner liners 12 are designed, thus... Figure 1 As shown, with the insulating base 1 as the center, an insulating inner liner 12, a first heat-conducting component 2, and an insulating outer shell 11 are sequentially assembled on the first side of the insulating base 1. An insulating inner liner 12, a second heat-conducting component 3, and an insulating outer shell 11 are symmetrically assembled on the second side of the insulating base 1. An insulating backing 13 is fixed to the rear side of the insulating base 1 away from the clamping space 4.

[0055] In one embodiment, such as Figure 1 and Figure 2As shown, the insulating backing 13 has a first through hole 131 and a second through hole 132. The electrode structure also includes a first plug 14 that passes through the first through hole 131 and connects to the first heat-conducting component 2, and a second plug 15 that passes through the second through hole 132 and connects to the second heat-conducting component 3. That is, the insulating backing 13 is fixed to the back of the insulating base 1, the first plug 14 passes through the first through hole 131 of the insulating backing 13 and is inserted into the first heat-conducting component 2 or the first heat-conducting block 21, and the second plug 15 passes through the second through hole 132 of the insulating backing 13 and is inserted into the second heat-conducting component 3 or the second heat-conducting block 31. Understandably, the insulating backing 13 may also be provided with a third through hole and a fourth through hole. The third temperature probe 91 passes through the third through hole of the insulating backing 13 and is inserted into the first heat-conducting component 2 or the first heat-conducting block 21 at a position close to the positive temperature change end 53 of the first cooling chip 51. The fourth temperature probe 92 passes through the fourth through hole of the insulating backing 13 and is inserted into the second heat-conducting component 3 or the second heat-conducting block 31 at a position close to the positive temperature change end 53 of the second cooling chip 52.

[0056] An electroporation electrode assembly includes an electroporation cup and the aforementioned electrode structure. The structure and working principle of this electrode structure are as described in the above embodiments and will not be repeated here. Understandably, in some embodiments, the electroporation electrode assembly may also include a control system. In some embodiments, the electroporation electrode assembly may also be communicatively connected to the control system, thereby allowing the control system to control the electroporation electrode assembly to perform electroporation on cells within the electroporation cup 10.

[0057] In the above embodiments of this utility model, the first heat-conducting component 2 and the second heat-conducting component 3 of the electrode structure form the two poles of a conductive electrode after being energized. The first heat-conducting component 2 and the second heat-conducting component 3 are insulated from each other by an insulating base 1, ensuring the safety of the electrode structure after it is energized. Furthermore, the clamping space 4 between the first heat-conducting component 2 and the second heat-conducting component 3 is used to clamp the electroporation cup 10. After the electroporation cup 10 is clamped in the clamping space 4, it can be connected to the two poles of the conductive electrode formed by the electrode structure, thereby enabling the conduction of electricity and the input of electrical pulses to the positive and negative plates of the electroporation cup 10, and thus achieving electroporation of the cells inside the cup 10. When the temperature-changing component 5 starts cooling, the positive temperature-changing end 53 of the temperature-changing component 5, which is connected to the first heat-conducting component 2 and the second heat-conducting component 3, begins cooling. The cooling capacity is transferred from the positive temperature-changing end 53 through the first heat-conducting component 2 and the second heat-conducting component 3 to the positive and negative electrode plates on both sides of the electroporation cup 10, thereby cooling the electroporation cup 10 and the cell fluid inside the cup. Furthermore, when the temperature of the cell fluid in the electroporation cup 10 is too low, the temperature-changing component 5 can also heat the electroporation cup 10 and the cell fluid inside the cup until the cell fluid in the electroporation cup 10 is heated to a certain temperature range before electroporation, thereby improving the electroporation effect and the cell survival rate in the cell fluid. At the same time, the first temperature detection component 6, installed on the first heat-conducting component 2 and / or the second heat-conducting component 3, is positioned opposite to the clamping space 4 and is used to detect the load end temperature of the electroporation cup 10 in real time. This allows the operating state of the temperature-changing component 5 to be controlled according to the measured load end temperature, so as to keep the load end temperature in a constant state. The electrode structure described above can control the load end temperature of the electric rotary cup 10 in a timely and stable manner, ensuring that the load end temperature is in a constant temperature state. Moreover, its structure is simple, its cost is low, its operation is simple, and it is easy to standardize.

[0058] In one embodiment, the electroporation process for cells in an electroporation cup according to this invention is as follows:

[0059] The electroporation cup 10 (electroporation carrier) is inserted into and held in the clamping space 4. The positive and negative plates on both sides of the electroporation cup 10 are connected to the two poles of the conductive electrodes formed by the electrode structure. After the electroporation cup 10 is inserted to the preset insertion position, it contacts the limit switch 8. The limit switch 8 prompts the user that the electroporation cup 10 has been inserted in place through a mechanical sound. The first plug 14 and the second plug 15 respectively input electrical pulses to the two poles of the conductive electrodes. At this time, there are electrical pulses between the positive and negative plates on both sides of the electroporation cup 10, thereby electroporating the cells inside the electroporation cup 10.

[0060] The refrigeration temperature control process in one embodiment of this utility model is as follows (the temperature control process during heating can be referred to the refrigeration temperature control process, and will not be repeated here):

[0061] When the temperature-changing component 5 starts cooling, it contacts the positive temperature-changing end 53 of the first heat-conducting block 21 and the second heat-conducting block 31 for cooling. The cooling capacity is transferred through the first heat-conducting block 21 (and the second heat-conducting block 31), the third heat-conducting block 222 (and the fourth heat-conducting block 322), and the first elastic heat-conducting component 22 (and the second elastic heat-conducting component 32) to the positive and negative plates on both sides of the electric rotating cup 10, thereby cooling the electric rotating cup 10 and the cell liquid inside the cup. The third temperature probe 91, inserted into the first heat-conducting block 21, is close to the positive temperature-changing end 53 of the first cooling chip 51, and the fourth temperature probe 92, inserted into the second heat-conducting block 31, is close to the positive temperature-changing end 53 of the second cooling chip 52. The temperature of the positive temperature-changing end 53 measured by the third temperature probe 91 and the fourth temperature probe 92 is fed back to the control system. The first temperature probe 61, inserted in the first limiting slot 223, is close to the positive plate of the electric rotating cup 10, and the second temperature probe 62, inserted in the second limiting slot 323, is close to the negative plate of the electric rotating cup 10. The load end temperature measured by the first temperature probe 61 and the second temperature probe 62 is fed back to the control system. Furthermore, based on the aforementioned forward temperature-changing end 53 temperature and load end temperature, the control system can maintain the load end temperature at a constant temperature. For example, when the load end temperature reaches the set temperature of the control system, the first cooling element 51 and the second cooling element 52 can be controlled to reduce power or stop working directly. Understandably, while the first cooling element 51 and the second cooling element 52 are working, the heat sink 7 is attached to the reverse temperature-changing end 54 of the first cooling element 51 and the second cooling element 52, and dissipates heat from the reverse temperature-changing end 54.

[0062] The above-described embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model, and should all be included within the protection scope of this utility model.

Claims

1. An electrode structure, characterized in that, include: An insulating base, comprising a first side and a second side disposed opposite to each other; A first heat-conducting component is installed on the first side of the insulating base; The second heat-conducting component is installed on the second side of the insulating base; the second heat-conducting component and the first heat-conducting component are arranged opposite to each other and form a clamping space for clamping the electric rotary cup; A temperature-changing component, including a positive temperature-changing end connected to the first heat-conducting component and the second heat-conducting component; A first temperature sensing element is installed on the first heat-conducting component and / or the second heat-conducting component at a position opposite to the clamping space, for detecting the load end temperature of the electric rotary cup.

2. The electrode structure according to claim 1, characterized in that, The temperature-changing element further includes a reverse temperature-changing end, and the electrode structure further includes a heat sink connected to the reverse temperature-changing end; and / or The electrode structure also includes a limit switch mounted on the insulating base and extending into the clamping space.

3. The electrode structure according to claim 1, characterized in that, The first heat-conducting component includes a first heat-conducting block mounted on the first side and connected to the positive temperature-changing end, and a first elastic heat-conducting element disposed on the first heat-conducting block; The second heat-conducting component includes a second heat-conducting block mounted on the second side and connected to the positive temperature-changing end, and a second elastic heat-conducting element disposed on the first heat-conducting block; The first elastic heat-conducting element and the second elastic heat-conducting element are arranged opposite to each other to form the clamping space.

4. The electrode structure according to claim 3, characterized in that, The first heat-conducting block is provided with a first sliding groove, and the first elastic heat-conducting element includes a first elastic element and a third heat-conducting block; the third heat-conducting block is slidably installed in the first sliding groove through the first elastic element; The second heat-conducting block is provided with a second sliding groove, and the second elastic heat-conducting element includes a second elastic element and a fourth heat-conducting block; the fourth heat-conducting block is slidably installed in the second sliding groove through the second elastic element.

5. The electrode structure according to claim 4, characterized in that, The first groove is provided with a first positioning post, the end face of the third heat-conducting block opposite to the fourth heat-conducting block is recessed to form a first groove, and a second positioning post is provided in the first groove at a position opposite to the first positioning post; the first elastic element is a first spring, and the opposite ends of the first spring are respectively sleeved in the first positioning post and the second positioning post; The second groove is provided with a third positioning post, and the end face of the fourth heat-conducting block opposite to the third heat-conducting block is recessed to form a second groove. The fourth positioning post is provided in the second groove at a position opposite to the third positioning post. The second elastic element is a second spring, and the opposite ends of the second spring are respectively sleeved in the third positioning post and the fourth positioning post.

6. The electrode structure according to claim 3, characterized in that, The first elastic heat-conducting element has a first guide slope and a first clamping surface connected to the first guide slope on its end face relative to the second elastic heat-conducting element, and the first clamping surface has at least one first rib; the second elastic heat-conducting element has a second guide slope and a second clamping surface connected to the second guide slope on its end face relative to the first elastic heat-conducting element, and the second clamping surface has at least one second rib; and / or The first elastic heat-conducting component has a first limiting groove on its end face facing the second elastic heat-conducting component, and the second elastic heat-conducting component has a second limiting groove on its end face facing the first elastic heat-conducting component; the first temperature detection component includes a first temperature probe installed in the first limiting groove and a second temperature probe installed in the second limiting groove.

7. The electrode structure according to claim 1, characterized in that, The temperature-changing component includes a first cooling element that is bonded and connected to the first heat-conducting component, and a second cooling element that is bonded and connected to the second heat-conducting component. The electrode structure further includes a third temperature probe mounted on the first heat-conducting component for detecting the temperature at the positive temperature-changing end of the first cooling chip, and a fourth temperature probe mounted on the second heat-conducting component for detecting the temperature at the positive temperature-changing end of the second cooling chip.

8. The electrode structure according to claim 1, characterized in that, The electrode structure further includes an insulating shell, an insulating inner liner, and an insulating backing. The first and second heat-conducting components are mounted on the insulating base via the insulating inner liner. The insulating backing is mounted on the side of the insulating base away from the clamping space. The insulating shell covers the surfaces of the first and second heat-conducting components.

9. The electrode structure according to claim 8, characterized in that, The insulating backing is provided with a first through hole and a second through hole. The electrode structure also includes a first plug that passes through the first through hole and connects to the first heat-conducting component, and a second plug that passes through the second through hole and connects to the second heat-conducting component.

10. An electroporation electrode assembly, characterized in that, It includes an electrospinning cup and an electrode structure as described in any one of claims 1 to 9.

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