Detachable tissue culture tweezers for callus inoculation subculture

Abstract

This utility model discloses a detachable tissue culture forceps for callus inoculation and subculture, which consists of one forceps (1) and one pair of inoculation spatulas (2). The forceps (1) is a common pointed, toothless straight forceps with a total length of 230 mm, a handle length of 200 mm, and a tip length of 30 mm. The outermost part of the handle has a 2 mm long oval protrusion, which serves as a connection slot between the forceps and the inoculation spatulas. The inoculation shovel consists of a handle (6), an inoculation platform (4), and an outer baffle (5). The handle (6) has a 2mm perforated interface (3) at the top, which can hold the long oval protrusion on the outer side of the lower part of the tweezers handle for connecting the inoculation shovel and the tweezers. The inoculation platform (4) is 20mm long and 10mm wide, and is used to support the callus tissue during inoculation and subculture operations. The wider inoculation platform is conducive to removing loose granular callus tissue at one time, which improves the working efficiency of plant tissue culture callus inoculation operations. The outer baffle (5) is 20mm long and 10mm high, which prevents the callus on the platform from slipping off the outside.

Description

Technical Field

[0001] This utility model relates to the field of plant tissue culture, specifically a detachable tissue culture forceps for callus inoculation and subculture. Background Technology

[0002] Inducing plant regeneration through callus culture is a routine technique in agricultural biotechnology. Callus induced from rice and barley seeds is relatively loose and granular. During inoculation and subculturing, operators need to use tweezers to pick out the smaller, more compact particles at the base of the callus cluster and transfer them to a new culture medium for further cultivation. Using ordinary pointed or flat-tipped tweezers to pick out granular callus is time-consuming, laborious, and inefficient in removing the desired callus. Furthermore, tissue culture is a sterile process, requiring frequent high-temperature sterilization of tools such as tweezers; both the sterilization time and the cooling time affect the efficiency of the tissue culture operation.

[0003] To address these issues, this invention develops a detachable tissue culture forceps for callus inoculation and subculture, characterized as follows:

[0004] This detachable tissue culture forceps is a modification of ordinary pointed forceps, incorporating a detachable inoculation spatula. The spatula platform expands the contact area between the forceps and the callus tissue, allowing for the removal of an entire clump of plant callus in one operation, thus improving the efficiency of inoculation and subculture of plant tissue culture callus. The detachable inoculation spatula is easy to manufacture, inexpensive, reusable, and convenient to replace. A batch of inoculation spatulas can be sterilized at high temperatures before tissue culture operations, and can be directly replaced during the operation, significantly saving operation time. It is highly suitable for large-scale, specialized production of plant tissue cultures such as rice and barley. Utility Model Content

[0005] This invention provides a detachable tissue culture forceps for callus inoculation and subculture. The forceps consists of one forceps (1) and one pair of inoculation spatulas (2).

[0006] The tweezers (1) and the inoculation spatula (2) are connected and disassembled through a long oval protrusion 2mm from the outer edge of the lower edge of the tweezers (1) and a hollow interface (3) 2mm above the inoculation spatula. The tweezers (1) are pointed, toothless straight tweezers. The inoculation spatula (2) consists of a handle (6), an inoculation platform (4), and an outer baffle (5). The inoculation platform (4) is 20mm long and 10mm wide. The outer baffle (5) is 20mm long and 10mm high. Both the pointed tweezers (1) and the inoculation spatula (2) are made of high-temperature resistant metal material.

[0007] The detachable tissue culture forceps can be used for inoculation and subculture of loose, granular callus tissues such as rice and barley.

[0008] The main advantages of this utility model are:

[0009] 1. Using an inoculation platform can greatly improve the efficiency of inoculation and subculture of loose, granular callus tissues such as rice and barley.

[0010] 2. A well-sterilized, detachable inoculation spatula can significantly shorten tissue culture operation time and reduce costs. Attached Figure Description

[0011] Other features, objects, and advantages of this application will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0012] Figure 1 This is a schematic diagram of the device of this utility model;

[0013] Figure 2 This is a schematic diagram of the inoculation spatula of this utility model;

[0014] Label Explanation:

[0015] 1: Pointed forceps; 2: Inoculation spatula; 3: Hollowed-out interface; 4: Inoculation platform; 5: Outer baffle; 6: Spatula handle. Detailed Implementation

[0016] The present invention will be further described below with reference to the accompanying drawings, but the present invention is not limited to the following embodiments.

[0017] This invention provides a detachable tissue culture forceps for callus inoculation and subculture, consisting of one forceps and one pair of inoculation spatulas. The forceps are pointed, toothless straight forceps, with a total length of 230mm, a handle length of 200mm, and a tip length of 30mm. The outermost part of the forceps handle has a 2mm elongated oval protrusion, serving as a connection slot between the forceps and the inoculation spatulas. The inoculation spatulas consist of a handle, an inoculation platform, and an outer baffle. The handle has a 2mm perforated interface that can hold the elongated oval protrusion on the outer side of the lower part of the forceps handle, facilitating the connection between the inoculation spatulas and the forceps. The inoculation platform is 20mm long and 10mm wide, used to support the callus during inoculation and subculture operations. The wider platform facilitates the removal of loose, granular callus in one go, improving the efficiency of plant tissue culture callus inoculation operations. The outer baffle is 20mm long and 10mm high, preventing callus from slipping off the platform.

[0018] Specific principles:

[0019] 1. Disinfection of inoculation spatulas: Disinfect several inoculation spatulas in an autoclave and set them aside for later use.

[0020] 2. Install the inoculation spatula: Take out a pair of inoculation spatulas on the clean bench, insert them into the two feet of the pointed forceps, and fix the inoculation spatula and forceps in place by inserting them into the long oval protrusion of the forceps through the hollow interface of the inoculation spatula.

[0021] 3. Tissue culture operation: (1) Take out a dish of rice callus tissue, hold the dish in one hand and pinch the tweezers with the other hand to bring the platform of the inoculation spatula together and then scoop up the granular callus tissue on the old culture medium. Then move the tweezers to transfer the callus tissue smoothly to the new culture medium. (2) When there is not much callus tissue left on the old culture medium, you can use the inoculation spatula to transfer them into the new culture dish.

[0022] 4. Replace the inoculation spatula: (1) Disconnect the hollowed-out interface of the inoculation spatula from the long oval protrusion of the tweezers and remove the inoculation spatula; (2) Take out a new inoculation spatula and continue the subculture operation of the callus tissue according to steps 2-3.

[0023] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Although the present utility model has been disclosed above with reference to a preferred embodiment, it is not intended to limit the present utility model. Any person skilled in the art can make modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present utility model's technical solution. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present utility model without departing from the scope of the present utility model's technical solution shall still fall within the scope of the present utility model's technical solution.

Claims

1. A detachable tissue culture tweezers for callus inoculation subculture, characterized in that, The tissue culture forceps consists of one forceps (1) and one pair of inoculation spatulas (2); the forceps (1) and the inoculation spatulas (2) are connected and disassembled through a long oval protrusion 2 mm outside the lower edge of the forceps (1) and a hollow interface (3) 2 mm above the inoculation spatulas; the forceps (1) is a pointed, toothless straight forceps; the inoculation spatulas (2) consists of a handle (6), an inoculation platform (4) and an outer baffle (5).

2. The tissue culture tweezer of claim 1, wherein, The inoculation platform (4) is 20mm long and 10mm wide; the outer baffle (5) is 20mm long and 10mm high.

3. The tissue culture forceps as described in claim 1, characterized in that, The tweezers (1) and the inoculation spatula (2) are both made of high-temperature resistant metal materials.

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