Stem cell gel cryo tube

Abstract

The utility model discloses a kind of stem cell gel cryopreservation tubes, including spiral cryopreservation tube and heat conduction sleeve, it is related to cryopreservation tube technical field, by being provided with spiral cryopreservation tube, the spiral storage cavity of spiral cryopreservation tube is formed by the spiral protrusion of outer tube body and hollow tube, so that stem cell gel forms continuous thin layer in cavity, substantially increase the contact area of gel and pipe wall, when freezing or recovery, heat can quickly penetrate entire gel layer, avoid the cell damage caused by excessive local temperature gradient, significantly improve cell activity retention rate, by being provided with heat conduction sleeve outside spiral cryopreservation tube, heat conduction sleeve is detachably set, heat jacket is embedded with the spiral recess of spiral cryopreservation tube through spiral protrusion, after installation, high thermal conductivity can be conducted cold quickly, avoid cold accumulation delay, improve rapid freezing efficiency, can be removed during conventional cryopreservation, adapt slow cooling demand.

Description

Technical Field

[0001] This utility model relates to the field of cryopreservation technology, specifically to a stem cell gel cryopreservation tube. Background Technology

[0002] In the field of stem cell research and clinical applications, the cryopreservation quality of stem cell gels directly affects cell viability and subsequent application outcomes. Currently used cryopreservation containers suffer from the following problems:

[0003] 1. Traditional cryovials are mostly cylindrical structures. After the stem cell gel is filled, it forms a thick columnar body. During freezing or thawing, the heat transfer path is long, which can easily lead to excessive local temperature gradients. The outer gel has completed freezing / thawing, while the core part is still in a slow change state, which may cause the cells to lose their activity due to low temperature damage or improper thawing.

[0004] 2. While existing glass cryopreservation tubes have good chemical stability, they cannot be processed into complex structures to optimize heat conduction, and they are prone to cracking due to thermal expansion and contraction at low temperatures; ordinary plastic cryopreservation tubes have problems with low thermal conductivity and insufficient molding precision, making it difficult to meet the stringent requirements of stem cell gels for cryopreservation environment.

[0005] 3. Different experimental scenarios have different requirements for freezing speed (e.g., conventional freezing requires slow cooling, while emergency freezing requires rapid freezing), but existing cryotubes lack adjustable heat conduction auxiliary structures and cannot flexibly adapt to various freezing needs. Utility Model Content

[0006] (a) Technical problems to be solved

[0007] To address the shortcomings of existing technologies, this invention provides a stem cell gel cryopreservation tube, which solves the aforementioned problems.

[0008] (II) Technical Solution

[0009] To achieve the above objectives, this utility model provides the following technical solution: a stem cell gel cryopreservation tube, comprising a spiral cryopreservation tube, wherein the spiral cryopreservation tube is composed of an outer tube body and a hollow tube, the bottoms of the outer tube body and the hollow tube are connected, the outer wall of the outer tube body is provided with a spiral recess, the spiral recess forming a spiral protrusion on the inner wall of the outer tube body, the inner wall of the hollow tube is provided with a spiral recess, the spiral recess forming a spiral protrusion on the outer wall of the hollow tube, and the spiral protrusion and the spiral protrusion forming a spiral storage cavity between the outer tube body and the hollow tube.

[0010] Preferably, the outer tube and the hollow tube are arranged concentrically.

[0011] Preferably, the top of the outer wall of the outer tube is provided with an external thread that is threaded with the sealing cap, and the sealing cap is used to seal the spiral storage cavity formed between the outer tube and the hollow tube.

[0012] Preferably, the outer side of the spiral cryopreservation tube is fitted with a heat-conducting sleeve, which includes an outer wall sleeve, an inner wall sleeve, a spiral protrusion three, and a spiral protrusion four. The inner wall sleeve is located in the middle of the outer wall sleeve, and the bottoms of the inner wall sleeve and the outer wall sleeve are connected. The inner wall of the inner wall sleeve is provided with a spiral protrusion three that matches the spiral recess one, and the outer wall of the inner wall sleeve is provided with a spiral protrusion four that matches the spiral protrusion two. The outer wall sleeve of the spiral cryopreservation tube is attached to the outer wall of the outer tube, and the inner wall sleeve is attached to the inner wall of the hollow tube.

[0013] Preferably, the outer wall sleeve inner wall is connected to the outer tube outer wall by a spiral protrusion three and a spiral recess one by a spiral thread, and the inner wall sleeve outer wall is connected to the hollow tube inner wall by a spiral protrusion four and a spiral recess two by a spiral thread.

[0014] Preferably, an observation port is provided at the bottom edge of the outer wall sleeve.

[0015] Preferably, the heat-conducting sleeve also includes a groove, an arc-shaped groove, and bolts. Grooves are provided on both the left and right sides of the bottom of the outer wall sleeve, and arc-shaped grooves are provided in the grooves. Bolt holes are provided on both the left and right sides of the bottom of the spiral cryopreservation tube. The bolts pass through the arc-shaped grooves and are threadedly connected to the bolt holes at the bottom of the spiral cryopreservation tube.

[0016] (III) Beneficial Effects

[0017] This invention provides a stem cell gel cryopreservation tube. It offers the following advantages: By incorporating a spiral cryopreservation tube, the spiral storage cavity is formed by the spiral protrusions of the outer tube and the hollow tube, allowing the stem cell gel to form a continuous thin layer within the cavity. This significantly increases the contact area between the gel and the tube wall. During freezing or thawing, heat can quickly penetrate the entire gel layer, avoiding cell damage caused by excessive local temperature gradients and significantly improving the cell viability retention rate.

[0018] This invention provides a stem cell gel cryopreservation tube. It offers the following advantages: A detachable heat-conducting sleeve is installed on the outside of the spiral cryopreservation tube. The sleeve engages with the spiral concave part of the cryopreservation tube via spiral protrusions. After installation, the high thermal conductivity allows for rapid cold transfer, preventing cold accumulation and delays, thus improving rapid freezing efficiency. It can be removed for regular cryopreservation, adapting to slow cooling requirements and achieving dual-purpose functionality. The spiral connection structure between the heat-conducting sleeve and the cryopreservation tube increases the heat exchange area. Combined with the bottom bolt for secure fixing, it ensures efficient heat conduction and prevents detachment after installation, improving operational stability. Attached Figure Description

[0019] Figure 1This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the internal structure of the present invention;

[0021] Figure 3 This utility model Figure 2 A magnified view of a portion of area A;

[0022] Figure 4 This is a schematic diagram of the spiral cryopreservation tube structure in this utility model;

[0023] Figure 5 This is a schematic diagram of the internal structure of the heat-conducting sleeve in this utility model;

[0024] Figure 6 This is a top view of the heat-conducting sleeve structure in this utility model;

[0025] Figure 7 This is a schematic diagram of the observation port structure in this utility model;

[0026] Figure 8 This is a bottom view of the bottom structure of the heat-conducting sleeve in this utility model.

[0027] In the diagram: Spiral cryopreservation tube-1, heat-conducting jacket-2, sealing cap-3;

[0028] Outer tube body-11, spiral recess-12, hollow tube-13, spiral protrusion one-14, spiral storage cavity-15, spiral recess two-16, bolt hole-17, spiral protrusion two-18;

[0029] Outer wall sleeve-21, inner wall sleeve-22, spiral protrusion three-23, spiral protrusion four-24, groove-25, arc groove-26, bolt-27, observation port-28. Detailed Implementation

[0030] The following is in conjunction with the appendix Figure 1 -Appendix Figure 8 This application will be described in further detail below.

[0031] Example 1: A stem cell gel cryopreservation tube, as described above. Figure 1-4 The system includes a spiral cryopreservation tube 1, which consists of an outer tube body 11 and a hollow tube 13. The bottoms of the outer tube body 11 and the hollow tube 13 are connected. The outer wall of the outer tube body 11 is provided with a spiral recess 12. The spiral recess 12 forms a spiral protrusion 14 on the inner wall of the outer tube body 11. The inner wall of the hollow tube 13 is provided with a spiral recess 16. The spiral recess 16 forms a spiral protrusion 18 on the outer wall of the hollow tube 13. The spiral protrusion 14 and the spiral protrusion 18 form a spiral storage cavity 15 between the outer tube body 11 and the hollow tube 13. The outer tube body 11 and the hollow tube 13 are arranged concentrically.

[0032] The spiral storage chamber 15 is non-isolated to avoid causing air bubbles when loading stem cell gel.

[0033] The top of the hollow tube 13 is closed to prevent the stem cell gel from being accidentally filled into the hollow tube 13 during filling.

[0034] The outer wall of the outer tube 11 is provided with an external thread that is threaded with the sealing cap 3. The sealing cap 3 is used to seal the spiral storage cavity 15 formed between the outer tube 11 and the hollow tube 13. A sealing ring is provided at the connection between the sealing cap 3 and the outer tube 11 and the hollow tube 13.

[0035] Spiral cryotube 1 is made of COC material, which is resistant to low temperatures. Compared with the commonly used glass cryotubes, COC has excellent thermoplastic fluidity and can be precisely injection molded into complex spiral grooves. Glass cryotubes cannot be processed into spiral structures and are prone to cracking at low temperatures.

[0036] The implementation principle of this application embodiment is as follows:

[0037] The stem cell gel is filled into the spiral storage cavity 15 formed by the spiral recess 12 and the spiral protrusion 18 between the outer tube 11 and the hollow tube 13. The sealing cap 3 is threaded to the outer tube 11 and the sealing cap 3 is tightened to seal the spiral storage cavity 15.

[0038] The continuous curved surface structure of the spiral storage chamber 15 greatly increases the contact area between the gel and the tube wall. The gel forms a thin layer inside the spiral storage chamber 15, and heat can quickly penetrate the entire gel layer during freezing or thawing, avoiding damage to cells from local temperature gradients.

[0039] Example 2: A stem cell gel cryopreservation tube, as described above. Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 8 The outer side of the spiral cryopreservation tube 1 is fitted with a heat-conducting sleeve 2. The heat-conducting sleeve 2 includes an outer wall sleeve 21, an inner wall sleeve 22, a spiral protrusion 3 23, and a spiral protrusion 4 24. The inner wall sleeve 22 is located in the middle of the outer wall sleeve 21, and the bottom of the inner wall sleeve 22 and the outer wall sleeve 21 are connected. The inner wall of the inner wall sleeve 22 is provided with a spiral protrusion 3 23 that fits into the spiral recess 12. The outer wall of the inner wall sleeve 22 is provided with a spiral protrusion 4 24 that fits into the spiral protrusion 2 18. The outer wall sleeve 21 of the spiral cryopreservation tube 1 is attached to the outer wall of the outer tube body 11, and the inner wall sleeve 22 is attached to the inner wall of the hollow tube 13.

[0040] The inner wall of the outer sleeve 21 is threaded to the spiral recess 12 of the outer wall of the outer tube 11 through the spiral protrusion 3 23, and the outer wall of the inner sleeve 22 is threaded to the spiral recess 26 of the inner wall of the hollow tube 13 through the spiral protrusion 4 24.

[0041] An observation port 28 is provided at the bottom edge of the outer sleeve 21, which is convenient for observing whether stem cell gel has flowed into the bottom of the spiral cryopreservation tube 1 when the heat-conducting sleeve 2 is installed on the outside of the spiral cryopreservation tube 1.

[0042] The heat-conducting sleeve 2 also includes a groove 25, an arc groove 26 and a bolt 27. The bottom left and right sides of the outer wall sleeve 21 are provided with grooves 25, and the grooves 25 are provided with arc grooves 26. The bottom left and right sides of the spiral cryopreservation tube 1 are provided with bolt holes 17. The bolts 27 pass through the arc grooves 26 and are threadedly connected to the bolt holes 17 at the bottom of the spiral cryopreservation tube 1.

[0043] The outer wall sleeve 21 and inner wall sleeve 22 are concentrically arranged with the outer tube body 11 and the hollow tube 13, and the arc groove 26 is arc-shaped. The arc groove 26 is opened with the center of the outer wall sleeve 21 as the center. When the outer wall sleeve 21 and inner wall sleeve 22 are fitted onto the outer wall of the outer tube body 11 and the inner wall of the hollow tube 13, the position of the arc groove 26 corresponds to the position of the bolt hole 17, so that the bolt 27 passes through the arc groove 26 and is bolted to the bolt hole 17, thereby locking and fixing the spiral cryopreservation tube 1 and the heat-conducting sleeve 2, preventing the heat-conducting sleeve 2 from easily falling off. In addition, the arc groove 26 is designed to allow for an angular deviation, ensuring that the position of the arc groove 26 corresponds to the bolt hole 17 when the spiral cryopreservation tube 1 and the heat-conducting sleeve 2 are connected.

[0044] The heat-conducting sleeve 2 is made of aluminum alloy, which has high thermal conductivity;

[0045] The implementation principle of this application embodiment is as follows:

[0046] When the heat-conducting sleeve 2 is connected to the spiral cryopreservation tube 1, the inner wall sleeve 22 of the heat-conducting sleeve 2 is screwed into the spiral recess 16 of the inner wall of the hollow tube 13 through the spiral protrusion 4 24. At the same time, the outer wall sleeve 21 of the heat-conducting sleeve 2 is screwed into the spiral recess 12 of the outer wall of the outer tube body 11 through the spiral protrusion 3 23.

[0047] Then, the bolt 27 is passed through the arc groove 26 at the bottom of the heat-conducting sleeve and locked into the bolt hole 17 at the bottom of the spiral cryopreservation tube 1 to lock and fix the spiral cryopreservation tube 1 and the heat-conducting sleeve 2.

[0048] Through the observation port 28 at the bottom of the heat-conducting sleeve, visually inspect whether the gel has filled the bottom of the spiral storage cavity 15 to avoid freezing failure due to insufficient filling;

[0049] When rapid freezing is required, install a heat-conducting jacket to accelerate cooling; remove the heat-conducting jacket for regular cryopreservation to suit different experimental needs.

[0050] Under the same conditions of freezing in liquid nitrogen:

[0051] 1. When the spiral cryotube 1 is not fitted with a heat-conducting sleeve 2, the low temperature of liquid nitrogen is conducted to the gel through the cryotube wall. Because the cryotube is directly exposed to liquid nitrogen, the low thermal conductivity leads to "delay in cold accumulation, resulting in low cryopreservation efficiency of the gel".

[0052] 2. When the heat-conducting sleeve 2 is installed on the outside of the spiral cryopreservation tube 1, the heat-conducting sleeve efficiently collects the cold energy of liquid nitrogen and then quickly transfers it to the gel through the tightly fitted spiral cryopreservation tube 1. The interlocking of the spiral protrusions 23 and 24 with the spiral recesses 12 and 16 of the spiral cryopreservation tube 1 further increases the heat exchange area, avoids the "cold energy accumulation delay" caused by the low thermal conductivity when the spiral cryopreservation tube 1 is directly exposed to liquid nitrogen, increases the heat conduction efficiency, and thus increases the cryopreservation efficiency of the gel.

[0053] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A stem cell gel cryopreservation tube, characterized in that: The device includes a spiral cryopreservation tube (1), which is composed of an outer tube body (11) and a hollow tube (13). The bottoms of the outer tube body (11) and the hollow tube (13) are connected. The outer wall of the outer tube body (11) is provided with a spiral recess (12), and the spiral recess (12) forms a spiral protrusion (14) on the inner wall of the outer tube body (11). The inner wall of the hollow tube (13) is provided with a spiral recess (16), and the spiral recess (16) forms a spiral protrusion (18) on the outer wall of the hollow tube (13). The spiral protrusion (14) and the spiral protrusion (18) form a spiral storage cavity (15) between the outer tube body (11) and the hollow tube (13).

2. The stem cell gel cryopreservation tube according to claim 1, characterized in that: The outer tube (11) and the hollow tube (13) are arranged concentrically.

3. The stem cell gel cryopreservation tube according to claim 1, characterized in that: The outer wall of the outer tube (11) is provided with an external thread that is threaded with the sealing cap (3). The sealing cap (3) is used to seal the spiral storage cavity (15) formed between the outer tube (11) and the hollow tube (13).

4. The stem cell gel cryopreservation tube according to claim 1, characterized in that: The spiral cryopreservation tube (1) is fitted with a heat-conducting sleeve (2) on its outer side. The heat-conducting sleeve (2) includes an outer wall sleeve (21), an inner wall sleeve (22), a spiral protrusion three (23), and a spiral protrusion four (24). The inner wall sleeve (22) is located in the middle of the outer wall sleeve (21), and the bottom of the inner wall sleeve (22) and the outer wall sleeve (21) are connected. The inner wall of the inner wall sleeve (22) is provided with a spiral protrusion three (23) that fits into the spiral recess one (12). The outer wall of the inner wall sleeve (22) is provided with a spiral protrusion four (24) that fits into the spiral protrusion two (18). The outer wall sleeve (21) of the spiral cryopreservation tube (1) is attached to the outer wall of the outer tube body (11), and the inner wall sleeve (22) is attached to the inner wall of the hollow tube (13).

5. A stem cell gel cryopreservation tube according to claim 4, characterized in that: The inner wall of the outer sleeve (21) is threaded to the spiral recess (12) of the outer wall of the outer tube (11) via a spiral protrusion three (23), and the outer wall of the inner sleeve (22) is threaded to the spiral recess (26) of the inner wall of the hollow tube (13) via a spiral protrusion four (24).

6. A stem cell gel cryopreservation tube according to claim 4, characterized in that: An observation port (28) is provided at the bottom edge of the outer wall sleeve (21).

7. A stem cell gel cryopreservation tube according to claim 4, characterized in that: The heat-conducting sleeve (2) also includes a groove (25), an arc groove (26) and a bolt (27). The bottom left and right sides of the outer wall sleeve (21) are provided with grooves (25), and the grooves (25) are provided with arc grooves (26). The bottom left and right sides of the spiral cryopreservation tube (1) are provided with bolt holes. The bolts (27) pass through the arc grooves (26) and are threadedly connected to the bolt holes at the bottom of the spiral cryopreservation tube (1).

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