Carotid sinus-sinus nerve preparation for perfusion and recording of action potentials in vitro

Abstract

The utility model relates to the technical field of biomedical experiment equipment discloses a kind of in-vitro carotid sinus-dome nerve specimen perfusion and discharge synchronous recording device, including the double-slot isolation structure being formed by blood vessel groove and nerve groove, perfusion system, nerve signal recording module and heat preservation module.Nerve groove partition plate is provided with narrow slit and sealed with silicon grease, blood vessel groove is provided with replaceable stainless steel cannula module, outside perfusion inlet and outlet of sinus, recording electrode is provided in nerve groove, integrated in thermostatic circulating liquid heat preservation box, can synchronously record sinus pressure and nerve discharge signal.

Description

Technical Field

[0001] This utility model relates to the field of biomedical experimental equipment technology, specifically an integrated device for simultaneous recording of perfusion and neural discharge for the study of isolated carotid sinus baroreceptors. Background Technology

[0002] Existing ex vivo carotid sinus research devices suffer from structural defects, operational complexity, and functional limitations. Manually prepared specimen tanks have poor sealing, are prone to leakage, and vibrations from the perfusion fluid and noise introduced by external equipment result in a low signal-to-noise ratio. Traditional devices require multiple reassemblies, making electrode and perfusion tube replacement difficult, resulting in poor adaptability, and they cannot achieve independent control of intravascular and extravascular perfusion or synchronous high-precision recording of pressure discharge signals.

[0003] In 2013, this device used a buffer system to control the intrasinus pressure, but it relied on a large-volume buffer bottle, resulting in a slow pressure regulation response. Although the improved device in 2018 adopted dual-slot isolation, it still suffered from problems such as unstable electrode fixation and insufficient heat preservation. Therefore, an integrated, high-precision, and easy-to-operate solution is urgently needed. Utility Model Content

[0004] To address the shortcomings of existing technologies, this utility model provides an integrated device that solves problems of leakage, signal interference, and cumbersome operation through structural innovation, enabling precise perfusion and synchronous recording of nerve discharge in an isolated carotid sinus pressure sensor.

[0005] The technical solution adopted by this utility model to solve its technical problem is as follows: a synchronous recording device for perfusion and discharge, comprising an insulated box body, an insulated box base, a quick-fit stainless steel cannula module, a nerve groove, and an interlocking insulating column, which together form a double-groove isolation structure, a perfusion system, a nerve signal recording module, and an insulated module. The insulated box body is provided with a vascular groove, and the nerve groove is installed inside; the two together are called a specimen groove, forming a double-groove isolation structure. A 0.5 mm narrow slit partition on the nerve groove separates the two grooves, which are sealed with silicone grease to achieve liquid isolation and electrical insulation. There are external perfusion inlets and outlets on both sides of the vascular groove. A quick-fit stainless steel cannula module is also provided on the top of the insulated box body for easy replacement of stainless steel cannulas of different diameters. Two guide grooves are provided at the external perfusion outlet of the box body, with a guide groove limiter between them to ensure the stability of the perfusion fluid level in the vascular groove. The insulated box and the base form a constant temperature circulating fluid insulated box with a built-in six-hole inner chamber. An insulating column is inserted from the bottom of the insulated box base, and two protrusions on its top are respectively placed with a reference electrode and a recording electrode. The reference electrode is located in the vascular groove, and the recording electrode is suspended in the nerve groove.

[0006] The aforementioned perfusion and discharge synchronous recording device has a uniform external sinus perfusion inlet and outlet on both sides of the vascular groove, with the outlet diameter being larger than the inlet diameter. Simultaneously, two external sinus perfusion fluid guide channels are provided at the outlet position, with guide channel limits directly between the two channels. Liquid entering the upper guide channel reaches a certain height and is blocked by the height limit before flowing into the lower guide channel, maintaining a stable liquid level within the vascular groove and forming an external sinus perfusion system.

[0007] In the aforementioned simultaneous perfusion and discharge recording device, the rapidly adaptable stainless steel cannula module is inserted into the slot of the insulation box. One stainless steel cannula is pre-placed on each side of the insulation box, with an outer diameter of 0.8 mm, 1.2 mm, and 1.5 mm. After passing through the stainless steel cannula modules with different outer diameters, they are connected to the common carotid artery end and the external carotid artery end of the specimen, respectively. An electric proportional regulating valve pushes the perfusion fluid through one side of the cannula into the common carotid artery, reaching the carotid sinus region and the external carotid artery, and out through the other side of the cannula, forming an intrasinus fluid perfusion system. The system is then connected to a pressure transducer to record the intrasinus pressure.

[0008] In the aforementioned simultaneous perfusion and discharge recording device, the nerve groove is placed inside the vascular groove. The groove is fixed inside the vascular groove by recording electrode protrusions on an insulating column and separated from the vascular groove by a septum with a 0.5 mm narrow slit. Together, they are referred to as the specimen groove. The sinus nerve from the common carotid artery can pass through the narrow slit and be fixed to the recording electrode on the insulating column. The narrow slit is sealed with silicone grease to achieve liquid isolation and electrical insulation.

[0009] The aforementioned simultaneous perfusion and discharge recording device includes a mating insulated column with two cylindrical protrusions at its top. One protrusion enters the vascular groove, while the other, after entering the vascular groove, enters the nerve groove through a circular notch at the bottom. The protrusion entering the vascular groove near the narrow slit of the nerve groove houses a stainless steel wire electrode, serving as a reference electrode for recording sinus nerve discharges. The protrusion entering the nerve groove also houses a stainless steel wire electrode, with an exposed portion approximately 6 mm long, suspended within the nerve groove, serving as a recording electrode. The nerve signal recording module, composed of the above structure, is connected to a data acquisition system via an amplifier after the electrode signals are received.

[0010] The above-mentioned perfusion and discharge synchronous recording device, the constant temperature circulating fluid insulation box, is composed of an insulation box body and an insulation box base to form an insulation module. The specimen tank is placed inside it to maintain the perfusion fluid temperature at 36~37℃. The insulation box base is provided with a six-hole anti-eddy current inner chamber design to buffer the fluid flow and reduce vibration.

[0011] The above-mentioned synchronous recording device for perfusion and discharge uses 3D printing to integrally form the heat preservation box body, heat preservation box base, quick-adaptive stainless steel insertion module, nerve slot and mating insulating column.

[0012] The beneficial effects of this invention are as follows: The perfusion and discharge synchronous recording device employs dual-groove isolation and silicone grease sealing technology to achieve liquid isolation and electrical insulation, effectively reducing external noise interference and improving the signal-to-noise ratio by more than 30%. The 3D-printed integrated groove, combined with a precision cannula interface design, completely eliminates sealing defects caused by manual assembly, avoids the risk of leakage, and allows for rapid adaptation to various animal models by changing different cannulas. The unique separation of the vascular groove and nerve groove achieves liquid isolation and electrical insulation. Different drugs or perfusion fluids can be introduced into the stainless steel cannula (for intrasinus perfusion) and the specimen groove (for extrasinus perfusion) respectively, supporting differential research inside and outside blood vessels. In particular, the unique inner chamber design of the constant-temperature circulating fluid insulated box effectively reduces perfusion fluid vibration, simulates the physiological environment, ensures the biological authenticity of experimental results, and further improves the signal-to-noise ratio. Attached Figure Description

[0013] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0014] Figure 1 This is a schematic diagram of the present invention;

[0015] Figure 2 For along Figure 1 A schematic diagram of AB in the diagram;

[0016] Figure 3 For along Figure 1 A schematic diagram of BB;

[0017] Figure 4 A schematic diagram of the stainless steel tubing module structure for quick adaptation;

[0018] Figure 5 Schematic diagram of the nerve groove structure

[0019] Figure 6 Schematic diagram of the interlocking insulating column structure

[0020] In the diagram: 1. Sinus perfusion fluid inlet; 2. Thermostatic circulating fluid outlet; 3. Anti-eddy current chamber of the insulation box; 4. Reference electrode; 5. Insulating column; 6. Insulation box body; 7. Sinus perfusion fluid outlet; 8. Guide channel limiter; 9. Upper guide channel; 10. Outlet stainless steel cannula; 11. Inlet stainless steel cannula; 12. Quick-fit stainless steel cannula module; 13. Insulation box; 14. Nerve groove; 15. Recording electrode; 16. Nerve groove partition slit (silicone grease seal); 17. Vascular groove; 18. Lower guide channel; 19. Insulation box base; 20. Guide channel outlet; 21. Thermostatic circulating fluid outlet; A. Sinus perfusion fluid; B. Thermostatic circulating fluid; C. Intrasinus perfusion fluid. Detailed Implementation

[0021]

Example 1

[0022] The isolated carotid sinus-sinus nerve specimen perfusion and discharge synchronous recording device includes an insulated box body 6, an insulated box base 19, a quick-fit stainless steel cannula module 12, a nerve groove 14, and an mateable insulating column 5. The device is manufactured using 3D printing technology. The insulated box body 6 is equipped with a vascular groove 17, and internally installed as follows... Figure 1 , Figure 2 , Figure 3 and Figure 5 The nerve groove 14 shown, together with the specimen groove, forms a double-groove isolation structure. The nerve groove 14 is separated by a 0.5 mm narrow slit 16, which is sealed with silicone grease to achieve liquid isolation and electrical insulation. The vascular groove 17 has an external perfusion inlet 1 and an outlet 7 on both sides. A quick-fit stainless steel cannula module 12 is also provided on the top of the insulated box body for easy replacement of stainless steel cannulas 10 of different diameters. An upper guide groove 9 and a lower guide groove 18 are provided at the external perfusion outlet 7 of the box body, with a guide groove limiter 8 between the guide grooves to ensure a stable perfusion fluid level in the vascular groove. The insulated box body 6 and the base 19 form a constant-temperature circulating fluid insulated box with a built-in six-hole inner chamber, such as... Figure 1 , Figure 2 , Figure 3 and Figure 6 The insulating column 5 shown penetrates from below the base 19 of the insulated box. The two protrusions on its top are respectively equipped with a reference electrode 4 and a recording electrode 15. The reference electrode 4 is located in the vascular groove 17, and the recording electrode 15 is suspended in the nerve groove 14.

[0023]

Example 2

[0024] The insulated box body 6 and the insulated box base 19 can be assembled into a complete constant temperature circulating fluid insulated box 13. The insulated box body 6 is provided with a vascular groove 17, and a uniform velocity external sinus irrigation inlet 1 and an outlet 7 are provided on both sides. The outlet diameter is larger than the inlet diameter. At the same time, an upper guide groove 9 and a lower guide groove 18 are provided at the outlet position. A guide groove limiter 8 is provided between the two guide grooves. The liquid entering the upper guide groove 9 reaches a certain height and flows into the lower guide groove 18 after being blocked by the height limiter, so as to keep the liquid level in the vascular groove 17 stable.

[0025]

Example 3

[0026] like Figure 1 , Figure 2 , Figure 3 and Figure 4The rapidly adaptable stainless steel cannula module 12 is inserted into the slot of the insulated box 6. Stainless steel cannulas can be inserted from both sides of the insulated box 6. Depending on the diameter of the internal tube of the rapidly adaptable stainless steel cannula module 12, the outer diameter of the cannula can be 0.8 mm, 1.2 mm, or 1.5 mm. After passing through the stainless steel cannula modules 12 with different outer diameters, they are connected to the common carotid artery end and the external carotid artery end of the specimen, respectively. The sinus nerve is introduced into the nerve groove 14 through the narrow slit 16, and the narrow slit 16 is sealed with high-viscosity silicone grease. Paraffin oil is injected into the nerve groove 14 to cover the recording electrode 15, and the surface of the perfusion fluid in the vascular groove 17 is covered with paraffin oil to prevent evaporation.

[0027]

Example 4

[0028] An electro-proportional regulating valve propels the perfusion fluid through a stainless steel cannula 11 into the common carotid artery, reaching the carotid sinus region and external carotid artery. The fluid then flows out through a stainless steel cannula 10 on the other side, connecting to a pressure transducer to record intrasinus pressure. The signal from the recording electrode 15 is amplified and transmitted to the data acquisition system, simultaneously storing the intrasinus pressure and discharge signal.

[0029]

Example 5

[0030] The constant temperature circulating fluid insulated box 13 consists of an insulated box body 6 and an insulated box base 19 forming an insulated module. The specimen tank is placed inside it to maintain the temperature of the perfusion fluid at 36~37℃. The insulated box base is equipped with a six-hole anti-vortex inner chamber 3 to buffer the fluid flow and reduce vibration.

[0031] 6. Conclusion of the Instruction Manual

[0032] This invention solves the problems of leakage, signal interference and complex operation of traditional devices through structural innovation and process optimization, and provides an efficient and accurate experimental platform for pressure sensor research.

Claims

1. A device for synchronous recording of perfusion and discharge of isolated carotid sinus-sinus nerve specimens, characterized in that, The system includes a dual-slot isolation structure, an irrigation system, a nerve signal recording module, and a heat preservation module. The dual-slot isolation structure consists of a vascular slot (17) and a nerve slot (14) separated by a partition with a narrow slit (16), which is sealed with silicone grease. The irrigation system includes intrasinus fluid irrigation and extrasinus fluid irrigation systems. The intrasinus fluid irrigation system consists of a vascular slot (17), a heat preservation box (6) with a quick-fit stainless steel cannula module (12) embedded in it, and replaceable stainless steel cannulas (10, 11) connected at both ends, used to adapt to different animal blood vessel sizes, with one end connected to a pulsating irrigation fluid. The other end is connected to a pressure transducer; the sinus external fluid perfusion system consists of a vascular groove (17) on the top of the heat preservation box (6) and a uniform speed sinus external perfusion inlet (1) and outlet (7) set on both sides of the box. An upper guide groove (9) and a lower guide groove (18) are set at the outlet position, and a guide groove limiter (8) is set between the two guide grooves; the nerve signal recording module includes a recording electrode (15) fixed in the nerve groove (14) by an insulating column (5), and the groove is filled with paraffin oil; the heat preservation module is a heat preservation box (13) with integrated constant temperature circulating fluid, which maintains the perfusion fluid temperature at 36~37℃.

2. The apparatus as claimed in claim 1, characterized in that, The width of the narrow slit (16) of the nerve groove (14) is 0.5 mm.

3. The apparatus as described in claim 1, characterized in that, The inner diameter and outer diameter of the stainless steel tubes (10, 11) that pass through the quick-fit stainless steel tube module (12) are 0.8 mm, 1.2 mm and 1.5 mm, respectively.

4. The apparatus as claimed in claim 1, characterized in that, The recording electrode (15) is a stainless steel wire with a diameter of 0.1 mm and an exposed part of 6 mm in length. It is fixed to the bottom of the nerve groove (14) by an insulating column (5).

5. The apparatus as claimed in claim 1, characterized in that, The sinus external perfusion inlet (1) and outlet (7) of the nerve groove are set independently. An upper guide groove (9) and a lower guide groove (18) are set at the outlet position. A guide groove limiter is set between the two guide grooves to ensure that the liquid level of the perfusion fluid in the vascular groove (17) is stable. Its surface is covered with paraffin oil to prevent evaporation.

6. The apparatus as claimed in claim 1, characterized in that... The heat preservation box (13) has a six-hole niche structure inside, which is the heat preservation box anti-vortex inner chamber (3).

7. The apparatus as claimed in claim 1, characterized in that, The replaceable stainless steel cannulas (10, 11) pass through stainless steel cannulas modules (12) with different outer diameters and are then connected to the common carotid artery end and the external carotid artery end of the specimen, respectively.

8. The apparatus as claimed in claim 1, characterized in that, The heat preservation box body (6), heat preservation box base (19), quick-fit stainless steel tube module (12), nerve groove (14), and mating insulating column (5) are 3D printed in one piece.

9. The apparatus as claimed in claim 1, characterized in that, The signal from the recording electrode (15) is transmitted to the data acquisition system via an amplifier, and the intrasinus pressure and nerve discharge signal are stored simultaneously.

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