A fully automatic cell fixing solution preparation device

Abstract

The utility model discloses a full -automatic cell fixing liquid configuration device belongs to the field of bioengineering technology, it includes the base, two vertical boards are connected on the base top, the stand is connected on the base top, the recess is seted up on the base top, the beaker is arranged in the recess, the mixing jar is fixedly installed on the stand, the measuring pump is fixedly installed on the both sides of stand top. This full -automatic cell fixing liquid configuration device, when the measuring pump can quantitatively extract solvent through the liquid outlet pipe, makes methanol and acetic acid to be injected into the mixing jar according to the proportion of 3:1 strictly, thereby reduced the error, and the motor can drive the connecting rod rotation, makes the stirring blade agitating solvent, makes methanol and acetic acid even mix, prevents the precipitation or delamination, promotes the stability and consistency of fixing liquid, in the process without manual participation, has guaranteed the accuracy, and the closed setting has prevented the gas volatilization, has protected the experimental personnel.

Description

Technical Field

[0001] This invention belongs to the field of bioengineering technology, specifically a fully automated cell fixation solution preparation device. Background Technology

[0002] Cell fixatives are used to preserve cell morphology and maintain excellent chromosome morphology and clarity. They are widely used in fields such as prenatal diagnosis in cytogenetics, chromosome karyotype analysis, fluorescence in situ hybridization, and cell division.

[0003] Cell fixatives are usually prepared by mixing methanol and acetic acid in a volume ratio of 3:1. Both methanol and acetic acid are hazardous chemicals, and routine preparation often relies on manual measurement and mixing, which can easily lead to errors in the ratio or uneven mixing, affecting the reproducibility of experiments. Although the preparation is carried out in a fume hood, volatile gases can still be generated, which can cause harm to the experimental personnel. Utility Model Content

[0004] To overcome the above-mentioned defects, this utility model provides a fully automated cell fixative preparation device, which solves the problems of uneven proportions caused by manual measurement and mixing and the harm to experimental personnel caused by volatile gases.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a fully automatic cell fixation solution preparation device, including a base, two upright plates connected above the base, a column connected above the base, a groove opened above the base, a beaker placed in the groove, a mixing tank fixedly installed on the column, a metering pump fixedly installed on both sides above the column, and a container connected to both sides of the column.

[0006] The container is connected to a liquid injection port at the top and a liquid outlet pipe at the bottom. The mixing tank is connected to a discharge pipe at the bottom. A motor is fixedly installed on the top of the mixing tank. The output shaft of the motor is connected to a connecting rod. Two connecting plates are connected to the connecting rod, and stirring blades are connected between the two connecting plates. An electric valve is installed on the discharge pipe.

[0007] As a further embodiment of this utility model: one end of the liquid outlet pipe is connected to a metering pump, one side of the metering pump is connected to a delivery pipe, and one end of the delivery pipe is connected to a mixing tank.

[0008] As a further embodiment of this utility model: a sealing plug is inserted into the injection port, and a graduated window is provided on the container.

[0009] As a further embodiment of this utility model: an anti-slip pad is connected inside the groove, the anti-slip pad is made of rubber and has a mesh pattern on its surface.

[0010] As a further embodiment of this utility model: the two upright plates on the base are specifically arranged on both sides of the groove, and a sliding rod is slidably connected through the upright plates, with a clamping plate connected to one end of the sliding rod.

[0011] As a further embodiment of this utility model: one side of the clamping plate is arc-shaped, the slide rod is T-shaped and sleeved with a spring, one end of the spring is connected to the end of the slide rod, and the other end of the spring is connected to the upright plate.

[0012] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0013] 1. This fully automated cell fixative preparation device consists of a container, a mixing tank, a metering pump, an outlet pipe, a delivery pipe, a connecting rod, and stirring blades. Placed inside a fume hood, the metering pump precisely extracts solvent through the outlet pipe, ensuring that methanol and acetic acid are injected into the mixing tank in a strict 3:1 ratio, thus reducing errors. The motor drives the connecting rod to rotate, causing the stirring blades to agitate the solvent, ensuring uniform mixing of methanol and acetic acid, preventing precipitation or stratification, and improving the stability and consistency of the fixative. The process requires no manual intervention, guaranteeing accuracy, and the sealed design prevents gas evaporation, protecting laboratory personnel.

[0014] 2. This fully automatic cell fixative preparation device is equipped with an anti-slip pad, a beaker, a sliding rod, a spring, and a clamping plate. By placing the beaker in the groove and contacting it with the anti-slip pad, the sliding rod is pulled, causing it to slide on the upright plate and stretch the spring, allowing the clamping plate to contact the beaker. The spring's rebound ensures a tight contact between the clamping plate and the beaker, thus clamping it. After the electric valve opens the discharge pipe, stable material reception is achieved, preventing accidental slippage and spillage. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the three-dimensional structure of the present invention;

[0016] Figure 2 This is a schematic diagram of the cross-sectional structure of the container cylinder of this utility model;

[0017] Figure 3 This is a schematic diagram of the cross-sectional structure of the mixing tank of this utility model;

[0018] Figure 4 This is a schematic diagram of the three-dimensional structure of the base of this utility model;

[0019] In the diagram: 1. Base; 2. Vertical plate; 3. Column; 4. Groove; 5. Beaker; 6. Mixing tank; 7. Metering pump; 8. Container; 9. Injection port; 10. Discharge pipe; 11. Discharge pipe; 12. Motor; 13. Connecting rod; 14. Connecting plate; 15. Stirring blade; 16. Electric valve; 17. Delivery pipe; 18. Sealing plug; 19. Scale window; 20. Anti-slip pad; 21. Slide rod; 22. Clamping plate; 23. Spring. Detailed Implementation

[0020] The technical solution of this patent will be further described in detail below with reference to specific embodiments.

[0021] like Figure 1-4 As shown, this utility model provides a technical solution: a fully automatic cell fixation solution preparation device, including a base 1, with two upright plates 2 connected above the base 1. The two upright plates 2 are set on both sides of a groove 4 on the base 1. A beaker 5 is placed in the groove 4, and an anti-slip pad 20 is connected in the groove 4. The anti-slip pad 20 is made of rubber and has a mesh pattern on its surface. Through the contact between the anti-slip pad 20 and the beaker 5, friction is increased, thereby improving the stability of the beaker 5. A sliding rod 21 is slidably connected through the upright plate 2. A clamping plate 22 is connected to one end of each sliding rod 21. One side of the clamping plate 22 is arc-shaped. The sliding rod 21 is T-shaped and fitted with a spring 23. One end of the spring 23 is connected to the end of the sliding rod 21, and the other end is connected to the upright plate 2. The spring force of the spring 23 allows the clamping plate 22 to tightly clamp and fix the beaker 5. The motor 12 is activated, causing the connecting rod 13 to drive the stirring blade 15 to agitate the solvent in the mixing tank 6, thereby improving mixing efficiency and uniformity without requiring manual operation by the experimenter. By pulling the sliding rod 21 or causing the beaker 5 to push the clamping plate 22, the sliding rod 21 slides on the upright plate 2, thus changing the distance between the two clamping plates 22, causing the clamping plates 22 to clamp and fix the beaker 5, preventing it from sliding.

[0022] A column 3 is connected to the top of the base 1, and a container 8 is connected to both sides of the column 3. A liquid injection port 9 is connected to the top of the container 8, and a sealing plug 18 is inserted into the liquid injection port 9. A scale window 19 is provided on the container 8. The sealing plug 18 can seal the liquid injection port 9 to prevent methanol and acetic acid from evaporating, while the scale window 19 on the container 8 can facilitate the experimenter to observe the remaining solvent.

[0023] Metering pumps 7 are fixedly installed on both sides above the column 3. A liquid outlet pipe 10 is connected to the bottom of the container 8. One end of the liquid outlet pipe 10 is connected to the metering pump 7, and the other side of the metering pump 7 is connected to the delivery pipe 17. One end of the delivery pipe 17 is connected to the mixing tank 6. The metering pump 7 extracts the solvent from the two containers 8 through the liquid outlet pipe 10, and then delivers it to the mixing tank 6 through the delivery pipe 17, thereby realizing quantitative mixing and improving accuracy.

[0024] A mixing tank 6 is fixedly installed on the column 3. A discharge pipe 11 is connected to the bottom of the mixing tank 6, and an electric valve 16 is installed on the discharge pipe 11. A motor 12 is fixedly installed on the top of the mixing tank 6. The output shaft of the motor 12 is connected to a connecting rod 13. The connecting rod 13 is located inside the mixing tank 6. Two connecting plates 14 are connected to the connecting rod 13, and a stirring blade 15 is connected between the two connecting plates 14.

[0025] The working principle of this utility model is as follows:

[0026] The device is placed in a fume hood. Methanol and acetic acid are injected into two containers 8 through the injection port 9, and then sealed with a sealing plug 18 to prevent evaporation. During preparation, the metering pump 7 can quantitatively extract the solvent through the outlet pipe 10 and inject methanol and acetic acid into the mixing tank 6 in a 3:1 ratio through the delivery pipe 17. This eliminates the need for manual measurement, reducing errors. The motor 12 drives the connecting rod 13 to rotate, causing the stirring blade 15 to agitate the solvent and promote uniform mixing of methanol and acetic acid, thus preventing precipitation or stratification and improving the stability and consistency of the solid solution. The beaker 5 is then placed in the groove 4 and contacts the anti-slip pad 20. During this process, by pulling the sliding rod 21 or causing the beaker 5 to push the clamping plate 22, the sliding rod 21 slides on the upright plate 2 and stretches the spring 23. The spring 23 then rebounds, causing the clamping plate 22 to make tight contact with the beaker 5, thus clamping it. After the electric valve 16 opens the discharge pipe 11, stable material reception is achieved.

[0027] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0028] The preferred embodiments of this patent have been described in detail above. However, this patent is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of this patent.

Claims

1. A fully automated cell fixative preparation device, comprising a base (1), characterized in that: Two upright plates (2) are connected above the base (1), and a column (3) is connected above the base (1). A groove (4) is opened above the base (1), and a beaker (5) is placed in the groove (4). A mixing tank (6) is fixedly installed on the column (3). A metering pump (7) is fixedly installed on both sides above the column (3). A container (8) is connected to both sides of the column (3). The container (8) is connected to a liquid injection port (9) at the top and a liquid outlet pipe (10) at the bottom. The mixing tank (6) is connected to a discharge pipe (11) at the bottom. A motor (12) is fixedly installed on the top of the mixing tank (6). The output shaft of the motor (12) is connected to a connecting rod (13). Two connecting plates (14) are connected to the connecting rod (13), and a stirring blade (15) is connected between the two connecting plates (14). An electric valve (16) is installed on the discharge pipe (11).

2. The fully automated cell fixation solution preparation device according to claim 1, characterized in that: One end of the outlet pipe (10) is connected to the metering pump (7), and one side of the metering pump (7) is connected to the delivery pipe (17). One end of the delivery pipe (17) is connected to the mixing tank (6).

3. The fully automated cell fixation solution preparation device according to claim 1, characterized in that: A sealing plug (18) is inserted into the injection port (9), and a scale window (19) is provided on the container (8).

4. The fully automated cell fixation solution preparation device according to claim 1, characterized in that: An anti-slip pad (20) is connected inside the groove (4). The anti-slip pad (20) is made of rubber and has a mesh pattern on top.

5. The fully automated cell fixation solution preparation device according to claim 1, characterized in that: The two upright plates (2) on the base (1) are specifically set on both sides of the groove (4). A sliding rod (21) is slidably connected through the upright plate (2), and a clamping plate (22) is connected to one end of the sliding rod (21).

6. The fully automated cell fixation solution preparation device according to claim 5, characterized in that: The clamp (22) has an arc-shaped design on one side, and the slide rod (21) has a T-shaped design and is fitted with a spring (23). One end of the spring (23) is connected to the end of the slide rod (21), and the other end of the spring (23) is connected to the upright plate (2).

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