An automatic deprotection and purification combined device for Fmoc protected amino acids

Abstract

The utility model relates to amino acid separation and purification technical field, concretely relates to a kind of automatic deprotection and purification combined device of Fmoc protected amino acid, including reaction kettle, stirring shaft is rotatably connected on the reaction kettle, and stirring blade is arranged in the inside of reaction kettle, which is extended to the lower end of stirring shaft;It further includes the ring that is movably connected in the inside position of the reaction kettle, multiple semi-permeable membrane pieces are arranged in ring shape equidistantly on the ring, and it further includes multiple filter membranes fixedly installed on the inner wall of the reaction kettle, filter cavity is formed between the middle portion of adjacent two filter membranes, magnetic block is fixedly installed on the inner wall of the filter cavity, and the magnetic block is magnetically adsorbed with the magnetic sheet fixedly installed on the side wall of semi-permeable membrane piece. Through the installation ring rotatably connected in the reaction kettle, in the purification process, semi-permeable membrane piece can be replaced in time, effectively avoid the influence of impurities on filtration effect, improve amino acid purification efficiency.

Description

Technical Field

[0001] This utility model relates to the field of amino acid separation and purification, and in particular to an automatic deprotection and purification device for Fmoc-protected amino acids. Background Technology

[0002] FMOC-protected amino acids are amino acid derivatives in which the functional groups of an amino acid react with other groups, blocking the activity of the functional groups. They are called protective amino acids. They include amino and carboxyl groups, as well as side-chain functional groups.

[0003] Currently, amino acids are simply separated and purified using semi-permeable membranes, but there are still some shortcomings. When separating and purifying amino acids using a semi-permeable membrane, the efficiency of separation and purification is related to the flow rate of the amino acids. A faster flow rate can easily lead to the accumulation of impurities on the surface of the semi-permeable membrane, thus affecting the filtration and separation effect of amino acids. In view of this, we designed an automatic deprotection and purification device for Fmoc-protected amino acids. Summary of the Invention

[0004] To address the aforementioned shortcomings of existing technologies, this invention provides an automated deprotection and purification device for Fmoc-protected amino acids. This device effectively solves the problem of existing methods that simply use semi-permeable membranes to separate and purify amino acids, but still has some deficiencies. When separating and purifying amino acids using a semi-permeable membrane, the efficiency of separation and purification is related to the flow rate of the amino acids. A faster flow rate can easily lead to excessive accumulation of impurities on the surface of the semi-permeable membrane, thereby affecting the filtration and separation effect of amino acids.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] An automated deprotection and purification device for Fmoc-protected amino acids includes a reaction vessel, a stirring shaft rotatably connected to the reaction vessel, and stirring blades extending from the lower end of the stirring shaft into the interior of the reaction vessel.

[0007] It also includes a ring movably connected to the inside of the reactor, on which a plurality of semi-permeable membranes are arranged in a ring at equal intervals. It also includes a plurality of filter membranes fixedly installed on the inner wall of the reactor, with a filter cavity formed between the middle of two adjacent filter membranes. A magnetic block is fixedly installed on the inner wall of the filter cavity, and the magnetic block is magnetically attracted to the magnetic sheet fixedly installed on the side wall of the semi-permeable membrane.

[0008] Preferably, the upper end of the stirring shaft passes through the reactor and is fixedly connected to the output end of an external motor, and a guide groove is provided on the outer wall of the stirring blade.

[0009] Preferably, multiple partition plates are fixedly installed in a ring at equal intervals on the inner wall of the reactor, and the partition plates are located on the inner side of the ring and provide a relative seal to the filter chamber. The outer wall of the partition plate overlaps with the inner wall of the ring.

[0010] Preferably, it also includes a liquid outlet pipe fixedly installed on the inner wall of the reactor, the liquid outlet pipe being in communication with the inside of the liquid outlet trough opened in the reactor, and the liquid outlet trough being located between the middle of two adjacent partition plates.

[0011] Preferably, it further includes a rotating shaft rotatably connected to the bottom surface of the reactor, a turntable fixedly mounted on the rotating shaft, and a magnetic ring fixedly mounted on the upper end of the turntable, the magnetic ring being magnetically attracted to a magnetic plate disposed on the bottom surface of the ring.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] This invention utilizes a rotating mounting ring installed inside the reactor to allow for timely replacement of the semi-permeable membrane during purification, effectively preventing impurities from affecting the filtration effect and improving the amino acid purification efficiency. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the overall structure of the purification and coupling device of the present invention;

[0016] Figure 2 This is a top view of the internal structure of the reactor of the present invention;

[0017] Figure 3 This is a schematic diagram of the overall exploded structure of the purification and coupling device of the present invention;

[0018] Figure 4 This is a schematic diagram of the reactor and rotating shaft of the present invention when separated.

[0019] Drawing number explanation:

[0020] 100. Reactor; 101. Filter chamber; 102. Discharge tank; 110. Filter membrane; 120. Magnetic block; 130. Discharge pipe; 140. Divider plate;

[0021] 200. Stirring shaft; 210. Stirring blades; 211. Guide groove;

[0022] 300. Ring; 310. Semi-permeable membrane; 311. Magnetic sheet;

[0023] 400, rotating shaft; 410, turntable; 420, magnetic ring. Detailed Implementation

[0024] The present invention will now be described in further detail with reference to the accompanying drawings.

[0025] The following description is intended to disclose the invention so that those skilled in the art can implement it. The preferred embodiments described below are merely examples, and other obvious modifications will be apparent to those skilled in the art. The basic principles of the invention defined in the following description can be used in other embodiments, modifications, improvements, equivalents, and other technical solutions that do not depart from the spirit and scope of the invention.

[0026] Those skilled in the art should understand that, in the disclosure of this invention, the terms "longitudinal," "lateral," "upper," "lower," "left," "right," "front," "rear," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or position based on the orientation or positional relationship shown in the accompanying drawings. They are merely simplified descriptions for the convenience of describing this invention and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the above terms should not be construed as limitations on this invention.

[0027] It is understood that the term "a" should be understood as "at least one" or "one or more", that is, in one embodiment, the number of an element can be one, while in another embodiment, the number of the element can be multiple, and the term "a" should not be understood as a limitation on the number. Example

[0028] See attached document Figure 1-4 As shown, an automated deprotection and purification device for Fmoc-protected amino acids includes a reaction vessel 100, a stirring shaft 200 rotatably connected to the reaction vessel 100, and stirring blades 210 extending from the lower end of the stirring shaft 200 into the reaction vessel 100. In the actual separation and purification process, the amino acid to be purified is placed inside the reaction vessel 100. The stirring shaft 200 rotatably connected to the reaction vessel 100 and the stirring blades 210 fixedly installed on the lower side of the stirring shaft 200 are used to stir the liquid inside the reaction vessel 100. During this process, the rotation of the stirring blades 210 drives the unidirectional flow of the liquid inside the reaction vessel 100, and the centrifugal force generated by the flowing liquid also causes it to flow towards the outside of the reaction vessel 100.

[0029] Furthermore, this application also includes a ring 300 movably connected to the inside of the reactor 100. Multiple semi-permeable membranes 310 are arranged in a ring at equal intervals on the ring 300. It also includes multiple filter membranes 110 fixedly installed on the inner wall of the reactor 100. A filter chamber 101 is formed between the middle of two adjacent filter membranes 110. When the liquid rotates inside the reactor 100, the corresponding liquid generates centrifugal force. During this process, the liquid continuously contacts the semi-permeable membranes 310 on the ring 300. Specifically, the semi-permeable membrane 310 mentioned in this application can be understood as a filter screen. When the liquid passes through the semi-permeable membrane 310, the corresponding impurities will remain on the outer wall of the semi-permeable membrane 310, while the amino acids can smoothly pass through the semi-permeable membrane 310 and enter the outlet tank 102 outside the reactor 100. The purified amino acids are collected using the outlet pipe 130 connected to the lower side of the outlet tank 102.

[0030] In one embodiment, in this application, the upper end of the stirring shaft 200 passes through the reactor 100 and is fixedly connected to the output end of an external motor. A guide groove 211 is provided on the outer wall of the stirring blade 210. By controlling the operating state of the external motor, the rotation speed of the stirring shaft 200 can be controlled, thereby controlling the magnitude of the centrifugal force of the liquid in the reactor 100 and achieving efficient filtration of amino acids. In particular, in this application, a guide groove 211 is also provided on the outer wall of the stirring blade 210. When the stirring blade 210 stirs the liquid in the reactor 100 under the action of the stirring shaft 200, the corresponding guide groove 211 will guide the liquid, thus ensuring that the liquid can flow in a direction and generate a relatively stable centrifugal force, avoiding damage to amino acids during the purification process.

[0031] Furthermore, in this application, multiple partition plates 140 are fixedly installed in a ring at equal intervals on the inner wall of the reactor 100, and the partition plates 140 are located inside the ring 300, providing a relative seal to the filter chamber 101. The outer wall of the partition plate 140 overlaps with the inner wall of the ring 300. It should be noted that in this application, the corresponding ring 300 is rotatably connected to the inside of the reactor 100, and the semi-permeable membranes 310 are linearly and equally spaced inside the ring 300. During the purification process, after the semi-permeable membranes 310 have been used for a period of time, impurities are prone to accumulate on the outer wall of the corresponding semi-permeable membranes 310. Therefore, in this application, after purification has been carried out for a period of time, the corresponding ring 300 can rotate relative to the reactor 100 to replace the semi-permeable membranes 310.

[0032] After use, the semi-permeable membrane 310 rotates to the position of the filter chamber 101. In this application, a magnetic block 120 is fixedly installed on the inner wall of the filter chamber 101. The magnetic block 120 and the magnetic sheet 311 fixedly installed on the side wall of the semi-permeable membrane 310 are magnetically attracted. During this process, it should be noted that when the magnetic sheet 311 and the magnetic block 120 are magnetically attracted, the shape of the corresponding semi-permeable membrane 310 will change. At this time, the through holes on the semi-permeable membrane 310 will increase, and impurities will enter the interior of the filter chamber 101. In this process, some amino acids will be carried in. Through the filter membranes 110 set on both sides of the filter chamber 101, the amino acids can be recovered again. The filter membrane 110 is a one-way filter, which allows the amino acids to pass through the filter membrane 110 and enter the interior of the outlet tank 102, avoiding contamination of the amino acids in the outlet tank 102.

[0033] This application also includes a liquid outlet pipe 130 fixedly installed on the inner wall of the reactor 100. The liquid outlet pipe 130 is in communication with the liquid outlet tank 102 located inside the reactor 100, and the liquid outlet tank 102 is located between the middle of two adjacent partition plates 140. By setting multiple partition plates 140, the liquid process at the position of the semi-permeable membrane 310 per unit time is avoided, which would cause poor filtration and purification effect.

[0034] Furthermore, this application also includes a rotating shaft 400 rotatably connected to the bottom surface of the reactor 100, a turntable 410 fixedly mounted on the rotating shaft 400, and a magnetic ring 420 fixedly mounted on the upper end of the turntable 410. The magnetic ring 420 is magnetically attracted to a magnetic plate disposed on the bottom surface of the ring 300. As one embodiment, this application provides a method for controlling the rotation of the ring 300. In this application, the rotating shaft 400, rotatably connected to the lower side of the reactor 100, is driven to rotate by an external drive mechanism, which in turn synchronously drives the magnetic ring 420 fixedly mounted on the turntable 410 to rotate. Combined with the magnetic attraction between the magnetic ring 420 and the magnetic plate on the bottom surface of the ring 300, the position of the ring 300 inside the reactor 100 is adjusted.

[0035] Those skilled in the art should understand that the embodiments of the present invention described above and shown in the accompanying drawings are merely examples and do not limit the present invention. The objectives of the present invention have been fully and effectively achieved. The functions and structural principles of the present invention have been shown and explained in the embodiments, and any modifications or variations of the embodiments of the present invention may be made without departing from the stated principles.

Claims

1. An apparatus for automatic deprotection and purification of Fmoc protected amino acids, characterized in that, include: A reaction vessel (100) is rotatably connected to a stirring shaft (200), and the lower end of the stirring shaft (200) extends into the interior of the reaction vessel (100) and is provided with stirring blades (210). It also includes a ring (300) movably connected to the inside of the reactor (100), on which a plurality of semi-permeable membranes (310) are arranged in a ring at equal intervals. It also includes a plurality of filter membranes (110) fixedly installed on the inner wall of the reactor (100), and a filter cavity (101) is formed between the middle of two adjacent filter membranes (110). A magnetic block (120) is fixedly installed on the inner wall of the filter cavity (101), and the magnetic block (120) is magnetically attracted to the magnetic sheet (311) fixedly installed on the side wall of the semi-permeable membrane (310).

2. The automatic deprotection and purification combined device for Fmoc protected amino acids according to claim 1, characterized in that: The upper end of the stirring shaft (200) passes through the reactor (100) and is fixedly connected to the output end of the external motor. The outer wall of the stirring blade (210) is provided with a guide groove (211).

3. The automated deprotection and purification device for Fmoc-protected amino acids according to claim 2, characterized in that: Multiple partition plates (140) are fixedly installed in a ring at equal intervals on the inner wall of the reactor (100), and the partition plates (140) are located on the inner side of the ring (300) and provide a relative seal to the filter chamber (101). The outer wall of the partition plate (140) overlaps with the inner wall of the ring (300).

4. The automated deprotection and purification device for Fmoc-protected amino acids according to claim 3, characterized in that: It also includes a liquid outlet pipe (130) fixedly installed on the inner wall of the reactor (100), the liquid outlet pipe (130) being in communication with the inside of the liquid outlet tank (102) opened in the reactor (100), and the liquid outlet tank (102) being located between the middle of two adjacent partition plates (140).

5. The automated deprotection and purification device for Fmoc-protected amino acids according to claim 4, characterized in that: It also includes a rotating shaft (400) rotatably connected to the bottom surface of the reactor (100), a turntable (410) is fixedly installed on the rotating shaft (400), a magnetic ring (420) is fixedly installed at the upper end of the turntable (410), and the magnetic ring (420) is magnetically attracted to a magnetic plate disposed on the bottom surface of the ring (300).

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