An apparatus for removing endotoxins from bacteriophages

Abstract

The utility model discloses a kind of remove phage endotoxin's device, belong to extraction filtration technical field, including frame body component, receiving platform component, dripping platform component, receiving pipe and drip pipe, the receiving platform component is set up with dripping platform component on frame body component, and dripping platform component is located the directly above of receiving platform component, the receiving pipe and drip pipe are placed respectively on receiving platform component and dripping platform component, and drip pipe is located the directly above of receiving pipe;The utility model designs a simple device for ion exchange resin material, the device does not need special equipment, just needs some simple experimental consumables and special aperture shelf, and simple operation, the requirement of operator is not high, combined with our operation process, the low-cost efficient removal of phage endotoxin can be completed.

Description

Technical Field

[0001] This utility model relates to the field of extraction and filtration technology, and in particular to a device for removing phage toxins. Background Technology

[0002] The equipment used for endotoxin removal varies depending on the removal method. These include activated carbon adsorption devices, ion exchange resin devices, ultrafiltration devices, chemical treatment devices, affinity chromatography devices, and plasma treatment devices. Activated carbon adsorption devices, using a packed bed configuration, utilize the large specific surface area and adsorption capacity of activated carbon to non-specifically adsorb endotoxins. While simple, they are bulky, adsorb other substances, require frequent material replacement, have high material costs, and exhibit low phage recovery efficiency. Ultrafiltration devices encompass ultrafiltration membrane modules, pumps, pressure sensors, and control systems. The pump provides power, the pressure sensor monitors pressure, and the control system adjusts parameters for continuous operation, but the equipment cost is high. In chemical treatment methods, acid-base reactions rely on reaction vessels made of glass or PTFE, such as beakers or flasks, equipped with stirrers. The device and pH meter are used to regulate the mixing of acid and alkali with the phage suspension and the pH. The subsequent dialysis is carried out by dialysis bags, which is time-consuming and labor-intensive. Affinity chromatography is based on the specific affinity of biomolecules, such as the binding of endotoxin and polymyxin B, which fixes the ligand on the chromatography medium. It has high specificity but is also expensive. Plasma treatment device destroys endotoxin with plasma field, which is efficient, fast and leaves no residue, but the equipment cost is high and the operation requirements are strict. Ion exchange resin device utilizes the charge interaction between resin and endotoxin. Different types are selected according to their charge characteristics, which have high selectivity and adsorption capacity.

[0003] This invention presents a simple device designed for ion exchange resin materials. This device does not require special equipment, only some simple experimental consumables and a frame with a special pore size. It is easy to operate and does not require high skill from the operator. Combined with the operating procedure, it can achieve low-cost and high-efficiency removal of toxins in bacteriophages. Utility Model Content

[0004] The present invention aims to provide a device for removing phage toxins, thereby solving the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] An apparatus for removing phage endotoxins includes a frame assembly, a receiving platform assembly, a dripping platform assembly, a receiving pipe, and a dripping pipe. The receiving platform assembly and the dripping platform assembly are disposed on the frame assembly, with the dripping platform assembly located directly above the receiving platform assembly. The receiving pipe and the dripping pipe are respectively placed on the receiving platform assembly and the dripping platform assembly, with the dripping pipe located directly above the receiving pipe.

[0007] The frame assembly includes two support side plates and a base. The two support side plates are symmetrically arranged on both sides of the base. The support side plates are provided with support grooves. The support grooves are composed of a longitudinal main groove and several transverse branch grooves. The branch grooves are arrayed on the main groove.

[0008] The receiving platform assembly includes an upper receiving plate, a lower receiving plate, and a support column. The lower receiving plate is fixedly mounted on the base, and the upper receiving plate is mounted on the lower receiving plate via the support column. The upper receiving plate has several first fixing holes, and the lower receiving plate has several positioning holes. The first fixing holes are located directly above the positioning holes, ensuring that the axes coincide.

[0009] The drip platform assembly includes an upper drip support plate, a lower drip support plate, and fixing screws. Threaded holes are provided on both sides of the upper and lower drip support plates. The upper and lower drip support plates are fixed to the frame assembly by the screws passing through the support grooves and engaging with the threaded holes. The upper drip support plate has several second fixing holes, and the lower drip support plate has several support holes. The second fixing holes are located directly above the support holes and ensure that the axes coincide.

[0010] The receiving pipe consists of a pipe body and a pipe cap. The pipe body is provided with scale lines and is placed on the receiving platform assembly through the first fixing hole. The bottom of the pipe body is a spherical surface that is used in conjunction with the positioning hole for vertical positioning.

[0011] The drip tube comprises a tube body, a cap, and a flow-limiting cap. The tube body contains filter media, and the bottom of the tube body is provided with a filter drip head. The top of the tube body is provided with an external thread, and the inner wall of the cap is provided with an internal thread. The cap is fixed to the tube body by the engagement of the internal and external threads. The top of the cap is provided with a flow-limiting tube, and the flow-limiting cap is threaded to the flow-limiting tube. The flow-limiting cap is provided with an air inlet.

[0012] The drip tube can be placed on the receiving platform assembly through the second fixing hole and the support hole, and the filter drip head passes through the support hole and faces the receiving tube.

[0013] The first fixing hole and positioning hole of the receiving platform component are vertically aligned with the second fixing hole and support hole of the dripping platform component.

[0014] Preferably, the frame is made of acrylic material.

[0015] Preferably, the filter material placed inside the drip tube is activated resin.

[0016] Preferably, the diameters of the first fixing hole and the positioning hole on the upper and lower support plates are respectively: the diameter of the first fixing hole is 3cm and the diameter of the positioning hole is 1.5cm.

[0017] Preferably, the diameters of the second fixing hole and the support hole on the upper drip support plate and the lower drip support plate are: the diameter of the second fixing hole is 3.3 cm, and the diameter of the support hole is 1.9 cm.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] This invention is a simple device designed for ion exchange resin materials. The device does not require special equipment, only some simple experimental consumables and a rack with a special pore size. It is easy to operate and does not require high skill from the operator. Combined with our operating procedure, it can achieve low-cost and high-efficiency removal of toxins in bacteriophages.

[0020] The device proposed in this invention is simple to assemble and inexpensive. From an economic perspective, it can significantly reduce experimental costs and enhance the market competitiveness of products. In terms of the production process, the device is easy to operate, can quickly remove endotoxins, effectively improve production efficiency, shorten the production cycle, and save time costs. From a technology promotion perspective, its low-cost characteristics enable more research institutions and enterprises to engage in phage-related research, strongly promoting the widespread application of phage technology in various fields such as biological control, medical treatment, and the food industry. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of a device for removing phage toxins.

[0022] Figure 2 A schematic diagram of the frame components of a device for removing phage endotoxins;

[0023] Figure 3 A schematic diagram of the receiving platform component structure of a device for removing phage endotoxins;

[0024] Figure 4 A schematic diagram of the upper drip support plate of a device for removing phage toxins;

[0025] Figure 5 A schematic diagram of the structure of the lower drip support plate of a device for removing phage toxins;

[0026] Figure 6 A schematic diagram of the support pipe structure of a device for removing phage endotoxins;

[0027] Figure 7 This is a diagram of the drip tube structure of a device for removing toxins from bacteriophages.

[0028] The reference numerals in the accompanying drawings include:

[0029] 1. Frame assembly; 101. Support side plate; 102. Base; 103. Support groove; 103a. Main groove; 103b. Branch groove; 2. Receiving platform assembly; 201. Upper receiving plate; 201a. First fixing hole; 202. Lower receiving plate; 202a. Positioning hole; 203. Support column; 3. Drip platform assembly; 301. Upper drip support plate; 301a. Second fixing hole; 302. Lower drip support plate; 302a. Support hole; 303. Fixing screw; 304. Threaded hole; 4. Receiving pipe; 401. Pipe body; 402. Pipe cap; 5. Drip pipe; 501. Pipe body; 501a. Filter drip head; 502. Cap; 503. Flow limiting cap. Detailed Implementation

[0030] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments:

[0031] like Figure 1-7 As shown, this utility model provides a device for removing phage toxins, including a frame assembly 1, a receiving platform assembly 2, a dripping platform assembly 3, a receiving pipe 4, and a dripping pipe 5. The receiving platform assembly 2 and the dripping platform assembly 3 are disposed on the frame assembly 1, and the dripping platform assembly 3 is located directly above the receiving platform assembly 2. The receiving pipe 4 and the dripping pipe 5 are respectively placed on the receiving platform assembly 2 and the dripping platform assembly 3, and the dripping pipe 5 is located directly above the receiving pipe 4.

[0032] The frame assembly 1 includes two support side plates 101 and a base 102. The two support side plates 101 are symmetrically arranged on both sides of the base 102. The support side plates 101 are provided with support grooves 103. The support grooves 103 are composed of a longitudinal main groove 103a and several transverse branch grooves 103b. The branch grooves 103b are arrayed on the main groove 103a.

[0033] The receiving platform component 2 includes an upper receiving plate 201, a lower receiving plate 202, and a support column 203. The lower receiving plate 202 is fixedly mounted on the base 102. The upper receiving plate 201 is mounted on the lower receiving plate 202 via the support column 203. The upper receiving plate has several first fixing holes 201a, and the lower receiving plate 202 has several positioning holes 202a. The first fixing holes 201a are located directly above the positioning holes 202a and are aligned with the axis. The diameter of the first fixing holes 201a is 3cm, and the diameter of the positioning holes 202a is 1.5cm.

[0034] The drip platform assembly 3 includes an upper drip support plate 301, a lower drip support plate 302, and fixing screws 303. Threaded holes 304 are provided on both sides of the upper drip support plate 301 and the lower drip support plate 302. The upper drip support plate 301 and the lower drip support plate 302 are fixed to the frame assembly 1 by the screws passing through the support groove 103 and engaging with the threaded holes 304. The upper drip support plate 301 has several second fixing holes 301a, and the lower drip support plate 302 has several support holes 302a. The second fixing holes 301a are located directly above the support holes 302a and are aligned with the axis. The diameter of the second fixing holes 301a is 3.3cm, and the diameter of the support holes 302a is 1.9cm.

[0035] The receiving pipe 4 consists of a pipe body 401 and a pipe cap 402. The pipe body 401 is provided with scale lines and can be placed on the receiving platform assembly 2 through the first fixing hole 201a. The bottom of the pipe body 401 is a spherical surface that is used in conjunction with the positioning hole 202a to vertically position it, ensuring that the pipe body 401 is in a vertical state and avoiding the situation where it tilts to the side and cannot receive the filtrate.

[0036] The drip tube 5 consists of a tube body 501, a cap 502, and a flow-limiting cap 503. The bottom of the tube body 501 is provided with a filter drip head 501a, and the top of the tube body 501 is provided with an external thread. The inner wall of the cap 502 is provided with an internal thread, which is fixed to the tube body 501 by the engagement of the internal and external threads. The top of the cap 502 is provided with a flow-limiting tube, and the flow-limiting cap 503 is threaded to the flow-limiting tube. The flow-limiting cap 503 is provided with an air inlet. By tightening and loosening the flow-limiting cap 503, the amount of air entering is controlled, thereby controlling the dripping flow rate of the drip tube 5.

[0037] The drip tube 5 has activated resin placed inside the tube body 501 as filter material;

[0038] The drip tube 5 can be placed on the receiving platform assembly 2 through the second fixing hole 301a and the support hole 302a, and the filter drip head 501a passes through the support hole 302a and faces the receiving tube 4.

[0039] The first fixing hole 201a and positioning hole 202a of the receiving platform component 2 are vertically aligned with the second fixing hole 301a and support hole 302a of the dripping platform component 3, thus ensuring that the dripping pipe 5 is located directly above the receiving pipe 4.

[0040] The specific implementation process is as follows:

[0041] First, remove the cap 402 of the receiving pipe 4 and place it directly on the receiving platform assembly 2 for collecting liquid. Fix the receiving pipe 4 through the first fixing hole 201a and vertically position it through the positioning hole 202a. Then, install the upper drip support plate 301 and the lower drip support plate 302 of the drip platform assembly 3 according to the applicable height. Adjust the height of the drip platform assembly 3 and the spacing between the upper drip support plate 301 and the lower drip support plate 302 by screws along the main groove 103a of the support groove 103. Fix the upper drip support plate 301 and the lower drip support plate 302 by screws through the horizontal branch groove 103b. Finally, place the drip tube 5 containing resin on the upper drip support plate 301 and the lower drip support plate 302 of the drip platform assembly 3. The filter dropper head 501a of the drip tube 5 passes through the support hole 302a and is suspended and aligned with the receiving pipe 4.

[0042] The apertures on the receiving platform component 2 and the dripping platform component 3 are custom-made to match the specifications of the receiving tube 4 and the dripping tube 5, ensuring a stable connection between them during experiments and effectively preventing spillage. The experimental process encompasses a series of operations, including resin activation, sample addition, and cleaning of the dripping tube 5, all of which are extremely simple. For example, adding resin is as simple as unscrewing the cap 502 of the dripping tube 5; controlling the flow rate is achieved by rotating the upper flow-limiting cap 503, without needing to remove the dripping tube 5 throughout the process. Discarding waste liquid is as simple as removing the receiving tube 4 from the third layer and emptying it. Before collecting the eluted phage solution, a new receiving tube 4 is used to collect the sample, thus successfully completing the phage endotoxin removal process and obtaining endotoxin-free phage samples.

[0043] The above descriptions are merely embodiments of this utility model. Commonly known technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solution of this utility model. These modifications and improvements should also be considered within the scope of protection of this utility model, and will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A device for removing phage endotoxins, characterized in that: It includes a frame assembly (1), a receiving platform assembly (2), a dripping platform assembly (3), a receiving pipe (4), and a dripping pipe (5). The receiving platform assembly (2) and the dripping platform assembly (3) are disposed on the frame assembly (1), and the dripping platform assembly (3) is located directly above the receiving platform assembly (2). The receiving pipe (4) and the dripping pipe (5) are respectively placed on the receiving platform assembly (2) and the dripping platform assembly (3), and the dripping pipe (5) is located directly above the receiving pipe (4). The frame assembly (1) includes two support side plates (101) and a base (102). The two support side plates (101) are symmetrically arranged on both sides of the base (102). The support side plates (101) are provided with support grooves (103). The support grooves (103) are composed of a longitudinal main groove (103a) and several transverse branch grooves (103b). The branch grooves (103b) are arrayed on the main groove (103a). The receiving platform assembly (2) includes an upper receiving plate (201), a lower receiving plate (202), and a support column (203). The lower receiving plate (202) is fixedly mounted on the base (102). The upper receiving plate (201) is mounted on the lower receiving plate (202) via the support column (203). The upper receiving plate has a plurality of first fixing holes (201a). The lower receiving plate (202) has a plurality of positioning holes (202a). The first fixing holes (201a) are located directly above the positioning holes (202a) and ensure that the axes coincide. The drip platform assembly (3) includes an upper drip support plate (301), a lower drip support plate (302), and fixing screws (303). Threaded holes (304) are provided on both sides of the upper drip support plate (301) and the lower drip support plate (302). The upper drip support plate (301) and the lower drip support plate (302) are fixed to the frame assembly (1) by the screws passing through the support groove (103) and engaging with the threaded holes (304). The upper drip support plate (301) has several second fixing holes (301a), and the lower drip support plate (302) has several support holes (302a). The second fixing holes (301a) are located directly above the support holes (302a) and ensure that the axes coincide. The receiving pipe (4) consists of a pipe body (401) and a pipe cap (402). The pipe body (401) is provided with scale lines and is placed on the receiving platform assembly (2) through the first fixing hole (201a). The bottom of the pipe body (401) is a spherical surface that is used to vertically position it with the positioning hole (202a). The drip tube (5) comprises a tube body (501), a cover (502), and a flow-limiting cover (503). The tube body (501) contains filter material. The bottom of the tube body (501) is provided with a filter drip head (501a). The top of the tube body (501) is provided with an external thread. The inner wall of the cover (502) is provided with an internal thread, which is fixed to the tube body (501) by the internal and external threads. The top of the cover (502) is provided with a flow-limiting tube. The flow-limiting cover (503) is threaded to the flow-limiting tube. The flow-limiting cover (503) is provided with an air inlet. The drip tube (5) can be placed on the receiving platform assembly (2) through the second fixing hole (301a) and the support hole (302a), and the filter drip head (501a) passes through the support hole (302a) and faces the receiving tube (4); The first fixing hole (201a) and positioning hole (202a) of the receiving platform component (2) are vertically aligned with the second fixing hole (301a) and support hole (302a) of the dripping platform component (3).

2. The device for removing phage endotoxins according to claim 1, characterized in that: The frame is made of acrylic.

3. The device for removing phage endotoxins according to claim 1, characterized in that: The filter material placed inside the drip tube (5) is activated resin.

4. The device for removing phage endotoxins according to claim 1, characterized in that: The diameters of the first fixing hole (201a) and the positioning hole (202a) on the upper support plate (201) and the lower support plate (202) are respectively: the diameter of the first fixing hole (201a) is 3cm and the diameter of the positioning hole (202a) is 1.5cm.

5. The device for removing phage endotoxins according to claim 1, characterized in that: The diameters of the second fixing hole (301a) and the support hole (302a) on the upper drip support plate (301) and the lower drip support plate (302) are as follows: the diameter of the second fixing hole (301a) is 3.3cm, and the diameter of the support hole (302a) is 1.9cm.

Images