A microplate sample adding automatic positioning device

Abstract

The utility model discloses a kind of microwell plate sample adding automatic positioning device, including the fixed placement mechanism for being positioned and limiting to 96 microwell plate, the mobile positioning mechanism for accurate positioning injection hole position is slidably arranged on the fixed plate, the mobile positioning mechanism includes two first sleeves respectively slidingly connected in the inside of two groups of limiting slot, the inside sliding connection of first sleeve has horizontal connecting rod, the inside of two groups of sliding slot is slidably connected with second sleeve, the inside sliding connection of second sleeve has vertical connecting rod, the positioning hole for aligning hole position is set up in the horizontal connecting rod and the vertical connecting rod, and the positioning hole is opened in the horizontal connecting rod and the vertical connecting rod in mobile positioning mechanism, the position of horizontal connecting rod and vertical connecting rod is adjusted, make positioning hole align target hole position, to provide accurate sample adding position for staff, avoid the problem such as missing, wrong hole addition caused by naked eye memory.

Description

Technical Field

[0001] This utility model relates to the field of microplate sample addition technology, specifically an automatic positioning device for microplate sample addition. Background Technology

[0002] Molecular biology techniques (such as CMA assays) have become important tools for the clinical diagnosis of genetic diseases. Molecular biology experiments involve multiple manual sample loading and pipetting operations, including pipetting from EP tubes to 96-well plates and from one 96-well plate to another.

[0003] In clinical practice, each sample is an independent individual (patient / subject), and the volume of sample added and pipetting is in the micro-level. Currently, laboratory personnel generally determine the designated position for pipetting by visual memory, which can lead to problems such as missing or adding to the wrong well.

[0004] To address these issues, this invention provides an automatic positioning device for microplate sample addition. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides an automatic positioning device for microplate sample addition, which solves the aforementioned problems.

[0006] To achieve the above objectives, this utility model is implemented through the following technical solution: an automatic positioning device for microplate sample addition, comprising a fixing and placement mechanism for positioning and limiting a 96 microplate, wherein the fixing and placement mechanism comprises a fixing plate, a limiting groove is provided on one corresponding side of the fixing plate, and a sliding groove is provided on the other corresponding side of the fixing plate;

[0007] The fixed plate is slidably provided with a moving positioning mechanism for accurately positioning the injection hole. The moving positioning mechanism includes two first sleeves that are slidably connected inside two sets of limiting grooves. A horizontal connecting rod is slidably connected inside the first sleeve. A second sleeve is slidably connected inside each of the two sets of sliding grooves. A vertical connecting rod is slidably connected inside the second sleeve. Positioning holes for aligning the injection hole are opened on both the horizontal connecting rod and the vertical connecting rod.

[0008] Preferably, the outer side of the first sleeve is provided with a threaded hole, and a knob is threadedly connected inside the threaded hole. A washer is fixedly connected to the side of the knob near the horizontal connecting rod, and an anti-slip groove is provided on the knob.

[0009] Preferably, the horizontal connecting rod has a sliding groove, and the vertical connecting rod is slidably disposed inside the sliding groove.

[0010] Preferably, the fixing plate is also provided with character markings that correspond to the character markings on the 96 micro-well plate.

[0011] Preferably, the bottom of the first sleeve is fixedly connected to a limiting mechanism for limiting the sliding of the moving positioning mechanism. The limiting mechanism includes an upper connecting plate fixedly connected to the bottom of the first sleeve, a spring fixedly connected to the bottom of the upper connecting plate, and a lower connecting plate fixedly connected to the bottom of the spring. The limiting groove is convex in shape and is divided into a small upper end and a large lower end. The lower connecting plate, the upper connecting plate, and the spring are all slidably disposed at the large lower end of the limiting groove, and the first sleeve is slidably disposed at the small upper end of the limiting groove.

[0012] Preferably, the inner wall of the larger end of the upper and lower parts of the limiting groove is provided with toothed grooves, and a toothed rack that matches the toothed grooves is fixedly connected to the upper connecting plate.

[0013] Preferably, the bottom of the lower connecting plate is rotatably connected with ball bearings to reduce the frictional force generated by the sliding of the lower connecting plate.

[0014] Preferably, a replaceable plastic film is provided in the positioning holes on both the horizontal connecting rod and the vertical connecting rod. Beneficial effects

[0015] This invention provides an automatic sample positioning device for microplate loading. Compared with the prior art, it has the following advantages:

[0016] (1) The automatic positioning device for microplate sample addition has a character mark corresponding to the microplate on the fixed plate of the fixed placement mechanism, which can ensure that the 96 microplate is placed in the correct position. The horizontal connecting rod and the vertical connecting rod in the moving positioning mechanism have positioning holes. By adjusting the position of the horizontal connecting rod and the vertical connecting rod, the positioning hole is aligned with the target hole, thereby providing the staff with an accurate sample addition position and avoiding problems such as missed addition or incorrect hole addition caused by visual memory.

[0017] (2) When the first sleeve is pressed, the upper connecting plate separates from the top wall of the lower large end of the limiting groove, and the horizontal connecting rod and the vertical connecting rod can be moved to the target hole. After being released, the spring returns to its original position and pushes the upper connecting plate to contact the top wall of the limiting groove. The rack of the upper connecting plate meshes with the tooth groove of the inner wall of the limiting groove, effectively fixing the position and preventing accidental collision that could cause positional deviation. At the same time, the ball bearings at the bottom of the lower connecting plate reduce the sliding friction, making the operation smoother. Attached Figure Description

[0018] Figure 1 This is a perspective view of the external structure of this utility model;

[0019] Figure 2 This is a schematic diagram showing the disassembled fixing mechanism and 96 microporous plate of this utility model;

[0020] Figure 3This is a cross-sectional schematic diagram of the fixing plate of this utility model;

[0021] Figure 4 This is a perspective view of the mobile positioning mechanism of this utility model;

[0022] Figure 5 This is a disassembled schematic diagram of the limiting mechanism of this utility model;

[0023] Figure 6 This is a split view of the limiting mechanism of this utility model from another perspective;

[0024] Figure 7 This is a schematic diagram showing the separation of the positioning hole and the plastic film in this utility model.

[0025] In the picture

[0026] 1. Fixed placement mechanism; 11. Fixed plate; 12. Limiting groove; 13. Sliding groove; 14. Tooth groove;

[0027] 2. Moving positioning mechanism; 21. First sleeve; 22. Horizontal connecting rod; 23. Sliding groove; 24. Knob; 25. Second sleeve; 26. Longitudinal connecting rod; 27. Positioning hole;

[0028] 3. 96 microplate;

[0029] Limiting mechanism; 41. Lower connecting plate; 42. Upper connecting plate; 43. Rack; 44. Spring; 45. Ball bearing;

[0030] Plastic film. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model. Example

[0032] Please see Figures 1 to 7 An automatic positioning device for microplate sample addition includes a fixing and placement mechanism 1 for positioning and limiting a 96 microplate 3. The fixing and placement mechanism 1 includes a fixing plate 11, a limiting groove 12 is provided on one side of the fixing plate 11, and a sliding groove 13 is provided on the other side of the fixing plate 11.

[0033] A movable positioning mechanism 2 for accurately positioning the injection hole is slidably disposed on the fixed plate 11. The movable positioning mechanism 2 includes two first sleeves 21 that are slidably connected inside the two sets of limiting grooves 12 respectively. A horizontal connecting rod 22 is slidably connected inside the first sleeve 21. A second sleeve 25 is slidably connected inside the two sets of sliding grooves 13. A longitudinal connecting rod 26 is slidably connected inside the second sleeve 25. Positioning holes 27 for aligning the injection hole are opened on both the horizontal connecting rod 22 and the longitudinal connecting rod 26.

[0034] In an optional embodiment: a replaceable plastic film 5 is provided in the positioning hole 27 on both the horizontal connecting rod 22 and the vertical connecting rod 26;

[0035] It should be noted that, in order to avoid the positioning holes 27 on the horizontal connecting rod 22 and the vertical connecting rod 26 being accidentally contaminated, the staff only need to remove the currently contaminated plastic film 5 and put on a new plastic film 5.

[0036] Furthermore, in order to avoid contamination of the positioning holes 27 on the horizontal connecting rod 22 and the vertical connecting rod 26 when the sample gun is applied to the 96 microplate 3, in actual use, the positioning hole 27 with an appropriate diameter should be selected according to the diameter of the sample gun tip (the diameter of the positioning hole 27 should be larger than the diameter of the sample gun tip to avoid contact between the two and cause contamination).

[0037] In an optional embodiment: a threaded hole is provided on the outer side of the first sleeve 21, and a knob 24 is threadedly connected inside the threaded hole. A gasket is fixedly connected to the side of the knob 24 near the horizontal connecting rod 22, and an anti-slip groove is provided on the knob 24.

[0038] It should be noted that the height of the horizontal connecting rod 22 should be adjusted according to the height of the 96 microplate 3. Once the height of the horizontal connecting rod 22 is determined, it can be fixed by tightening the knob 24. In this way, it can be reused without adjusting the height of the horizontal connecting rod 22 again when the 96 microplate 3 is not replaced.

[0039] In an optional embodiment: a sliding groove 23 is provided on the horizontal connecting rod 22, and the vertical connecting rod 26 is slidably disposed inside the sliding groove 23.

[0040] It should be noted that with this setting, after adjusting and fixing the height of the horizontal connecting rod 22, the height of the vertical connecting rod 26 can be adjusted and fixed synchronously through the horizontal connecting rod 22. At the same time, the staggered arrangement of the vertical connecting rod 26 and the horizontal connecting rod 22 can also make the thickness of the two combined not too thick, which is also beneficial for the staff's pipetting operation.

[0041] In an optional embodiment, the fixing plate 11 is further provided with character identifiers that correspond to the character identifiers on the 96 microporous plate 3.

[0042] It should be noted that this setting can prevent staff from reversing the position of the 96 microplate 3 on the fixing plate 11. At the same time, since the staff's pipetting operation usually takes a long time, this setting can prevent the text on the 96 microplate 3 from being too small or being blocked by the horizontal connecting rod 22 and the vertical connecting rod 26 when locating the corresponding hole. This would increase the workload of the staff.

[0043] In an optional embodiment: the bottom of the first sleeve 21 is fixedly connected to a limiting mechanism 4 for limiting the sliding of the moving positioning mechanism 2. The limiting mechanism 4 includes an upper connecting plate 42 fixedly connected to the bottom of the first sleeve 21, a spring 44 fixedly connected to the bottom of the upper connecting plate 42, a lower connecting plate 41 fixedly connected to the bottom of the spring 44, and a convex shape of the limiting groove 12, which is divided into a small end at the top and a large end at the bottom. The lower connecting plate 41, the upper connecting plate 42 and the spring 44 are all slidably disposed at the large end at the bottom of the limiting groove 12, and the first sleeve 21 is slidably disposed at the small end at the top of the limiting groove 12.

[0044] It should be noted that when it is necessary to find the corresponding hole position of the longitudinal connecting rod 26 through the horizontal connecting rod 22, the first sleeve 21 can be pressed down as a whole first. At this time, the upper connecting plate 42 will no longer contact the top wall of the lower large end of the limiting groove 12. Then the horizontal connecting rod 22 can be moved to the designated position, and then the first sleeve 21 can be released. The spring 44 will return to its original position, thereby pushing the upper connecting plate 42 to contact the top wall of the lower large end of the limiting groove 12, thus preventing the position of the horizontal connecting rod 22 from deviating due to accidental contact.

[0045] At the same time, a limiting mechanism 4 can also be set at the bottom of the longitudinal connecting rod 26 according to actual usage requirements, and the slide groove 13 can be set as a corresponding protrusion.

[0046] In an optional embodiment: a toothed groove 14 is provided on the inner wall of the larger end of the upper and lower parts of the limiting groove 12, and a toothed rack 43 that matches the toothed groove 14 is fixedly connected to the upper connecting plate 42.

[0047] It should be noted that the meshing of the rack 43 and the toothed groove 14 makes it more difficult for the upper connecting plate 42 to slide, which can better prevent the horizontal connecting rod 22 from moving due to accidental contact.

[0048] In an optional embodiment: the bottom of the lower connecting plate 41 is rotatably connected to a ball bearing 45 for reducing the frictional force generated by the sliding of the lower connecting plate 41;

[0049] It should be noted that this setting can reduce the friction between the lower connecting plate 41 and the bottom of the limiting groove 12 when the first sleeve 21 moves the lower connecting plate 41, thereby making the resistance of the first sleeve 21 less when it moves.

[0050] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

[0051] Working principle: During operation, the 96 microplate 3 is placed in the fixing plate 11 and aligned with the position by the character markings. Then, the height of the horizontal connecting rod 22 is adjusted and fixed with the knob 24. Pressing the first sleeve 21 moves the horizontal connecting rod 22 and the vertical connecting rod 26 to the target hole position. At this time, the positioning holes 27 on the horizontal connecting rod 22 and the vertical connecting rod 26 are aligned with the target hole position on the 96 microplate 3. After releasing the first sleeve 21, the spring 44 drives the upper connecting plate 42 to contact the top wall of the limiting groove 12. The position is fixed by the rack 43 and the toothed groove 14. Then, the operator can perform the sample addition operation.

[0052] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0053] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An automatic sample positioning device for microplate loading, characterized in that: It includes a fixing and placement mechanism (1) for positioning and limiting the 96 microporous plate (3). The fixing and placement mechanism (1) includes a fixing plate (11). A limiting groove (12) is provided on one side of the fixing plate (11), and a sliding groove (13) is provided on the other side of the fixing plate (11). The fixed plate (11) is slidably provided with a moving positioning mechanism (2) for accurately positioning the injection hole. The moving positioning mechanism (2) includes two first sleeves (21) that are slidably connected inside the two sets of limiting grooves (12). A horizontal connecting rod (22) is slidably connected inside the first sleeve (21). A second sleeve (25) is slidably connected inside the two sets of sliding grooves (13). A vertical connecting rod (26) is slidably connected inside the second sleeve (25). Positioning holes (27) for aligning the injection hole are opened on both the horizontal connecting rod (22) and the vertical connecting rod (26).

2. The automatic sample positioning device for microplates according to claim 1, characterized in that: The first sleeve (21) has a threaded hole on its outer side, and a knob (24) is threaded inside the threaded hole. A gasket is fixedly connected to the knob (24) on the side near the horizontal connecting rod (22), and an anti-slip groove is provided on the knob (24).

3. The automatic sample positioning device for microplates according to claim 1, characterized in that: The horizontal connecting rod (22) has a sliding groove (23), and the vertical connecting rod (26) is slidably disposed inside the sliding groove (23).

4. The automatic sample positioning device for microplates according to claim 1, characterized in that: The fixing plate (11) is also provided with character markings that correspond to the character markings on the 96 microporous plate (3).

5. The automatic sample positioning device for microplates according to claim 1, characterized in that: The bottom of the first sleeve (21) is fixedly connected to a limiting mechanism (4) for limiting the sliding of the moving positioning mechanism (2). The limiting mechanism (4) includes an upper connecting plate (42) fixedly connected to the bottom of the first sleeve (21). A spring (44) is fixedly connected to the bottom of the upper connecting plate (42). A lower connecting plate (41) is fixedly connected to the bottom of the spring (44). The limiting groove (12) is convex in shape and is divided into a small end at the top and a large end at the bottom. The lower connecting plate (41), the upper connecting plate (42) and the spring (44) are all slidably disposed at the large end at the bottom of the limiting groove (12). The first sleeve (21) is slidably disposed at the small end at the top of the limiting groove (12).

6. The automatic sample positioning device for microplates according to claim 5, characterized in that: The inner wall of the upper and lower ends of the limiting groove (12) is provided with toothed grooves (14), and a rack (43) that matches the toothed grooves (14) is fixedly connected to the upper connecting plate (42).

7. The automatic sample positioning device for microplates according to claim 5, characterized in that: The bottom of the lower connecting plate (41) is rotatably connected to a ball bearing (45) for reducing the friction generated by the sliding of the lower connecting plate (41).

8. The automatic sample positioning device for microplates according to claim 1, characterized in that: Replaceable plastic film (5) is provided in the positioning holes (27) on the horizontal connecting rod (22) and the vertical connecting rod (26).

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