A washing device for laboratory DNA extraction deep-well plates

Abstract

The utility model relates to medical instrument technical field, concretely relates to a kind of cleaning device of laboratory DNA extraction deep well plate, including bottom plate, bottom plate side fixedly connected with support plate, support plate top fixedly connected with top plate, and top plate bottom detachably connected with lifting assembly, and lifting assembly bottom fixedly connected with connecting plate, and connecting plate bottom detachably connected with several cleaning brushes;Bottom plate top detachably connected with deep well plate.The utility model cleaning assembly takes corresponding 96-hole brush with deep well plate, only need to wash once to complete the cleaning of a piece of deep well plate, reduce workload while also can guarantee that each hole of deep well plate can be cleaned, which will greatly improve work efficiency under the premise of guaranteeing quality.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, specifically to a cleaning device for deep-well plates used in laboratory DNA extraction. Background Technology

[0002] Currently, 96-well deep-well plates (corresponding to PCR plates) are commonly used in laboratories for batch extraction of plant tissue DNA. These deep-well plates are wear-resistant and heat-resistant, and need to be used for a long time. After extracting a batch of DNA, the problem of cleaning arises. Ultrasonic cleaners have been tried, but the results are not ideal, as residue often remains on the well walls, and ultrasonic cleaners cannot completely clean them. Currently, small brushes are often used to manually clean each well. This method ensures that each well is cleaned, but the problem is that the workload is greatly increased. One deep-well plate needs to be poked and washed 96 times. If DNA is extracted in large batches (population genotyping involves tens of thousands of plants), the cleaning of the deep-well plates alone will require a lot of effort.

[0003] Existing technology, Chinese Patent No. CN209452358U, discloses a deep-hole plate cleaning device, comprising a frame, a cleaning device, and a tilting device. The cleaning device includes a cover plate, cleaning brushes, and a first motor. The cover plate is horizontally positioned and mounted on the frame. The number of cleaning brushes corresponds to the number of holes in the deep-hole plate, and all cleaning brushes are vertically positioned. Each cleaning brush has a vertically positioned handle at its upper end, and each handle is connected to the output shaft of a first motor. The first motors are all fixedly mounted on the cover plate. The purpose of this invention is to provide a deep-hole plate cleaning device to solve the problem of inconvenient deep-hole plate cleaning.

[0004] However, in practical applications, the cleaning brush of the aforementioned deep hole plate cleaning device is fixedly installed on the device, which is convenient for cleaning deep hole plates with a fixed number of holes. However, it is inconvenient to clean deep hole plates with different numbers of holes. By making the cleaning brush detachable, it can be used to clean deep hole plates with different numbers of holes, thereby making the deep hole plate cleaning work more efficient and convenient. Utility Model Content

[0005] To address the aforementioned issues, this invention provides a cleaning device for deep-well plates used in laboratory DNA extraction. This device ensures the cleaning quality of the deep-well plates while improving cleaning efficiency, reducing workload, and increasing work efficiency.

[0006] To achieve the above objectives, the technical solution of this utility model is as follows: a cleaning device for deep well plates for laboratory DNA extraction, comprising a base plate, a support plate fixedly connected to one side of the base plate, a top plate fixedly connected to the top of the support plate, a lifting assembly detachably connected to the bottom of the top plate, a connecting plate fixedly connected to the bottom of the lifting assembly, and a plurality of cleaning brushes detachably connected to the bottom of the connecting plate.

[0007] A deep-hole plate is detachably connected to the top of the base plate.

[0008] The technical principle of the above solution is as follows: the lifting component drives the connecting plate to rise or fall, thereby allowing the cleaning brush to move in and out of the hole. The cleaning brush rubs against the inner wall of the hole, thereby automatically cleaning the deep hole plate placed on the base plate; alternatively, the lifting component, connecting plate and cleaning brush can be removed, and the experimenter can manually control the cleaning brush to move in and out of the hole to clean the deep hole plate.

[0009] The above approach has the following beneficial effects:

[0010] 1. This solution features a frame structure that can hold the deep hole plate, and a lifting assembly that moves the cleaning brush up and down to automatically clean the deep hole plate, greatly reducing the workload of staff.

[0011] 2. The detachable design of the lifting assembly and cleaning brush in this solution allows the cleaning brush to be removed and used separately to meet the cleaning needs of deep hole plates with different hole numbers; at the same time, it facilitates maintenance and repair when malfunctions occur or parts need to be replaced, reducing maintenance costs and difficulty.

[0012] 3. In this design, the bottom plate can be detachably connected to the deep hole plate, which makes it convenient for experimenters to quickly place the used deep hole plate to the cleaning position and remove it after cleaning.

[0013] Furthermore, the lifting assembly includes a housing, inside which an electric control cylinder is installed. The output shaft of the electric control cylinder extends towards the base plate to the outside of the housing and is fixedly connected to the top of the connecting plate.

[0014] Beneficial effects: The electric cylinder can precisely control the extension and retraction length of the output shaft, thereby accurately controlling the lifting and lowering position of the connecting plate, i.e. the cleaning brush. Through the back and forth extension and retraction of the output shaft of the electric cylinder, the cleaning brush is controlled to automatically clean the deep hole plate.

[0015] Furthermore, the number and position of the cleaning brushes correspond to the number and position of the deep hole plate, respectively.

[0016] Beneficial effects: The number and position of the cleaning brushes correspond to the number and position of the deep hole plate, ensuring that each deep hole has a corresponding cleaning brush for cleaning. This allows the entire deep hole plate to be cleaned at once, reducing workload and improving work efficiency.

[0017] Furthermore, the top of the base plate is provided with a placement groove that corresponds to the size of the bottom of the deep hole plate.

[0018] Beneficial effects: The placement slot provides precise positioning for the deep hole plate, ensuring that the deep hole plate is placed in the same position every time. In this way, the cleaning brush of the cleaning component can accurately align with each hole of the deep hole plate for cleaning, avoiding situations where incomplete cleaning or damage to the deep hole plate due to placement deviation of the deep hole plate.

[0019] Furthermore, fixing blocks are hinged to both sides of the placement slot, and torsion springs are provided at the hinge points between the fixing blocks and the base plate.

[0020] Beneficial effects: After the deep hole plate is placed in the placement slot, the fixing block can be rotated around the hinge point to press it against both sides of the deep hole plate. The torsion spring will generate torque, which will make the fixing block press the deep hole plate tightly, preventing the deep hole plate from shifting due to vibration, shaking or the force of the cleaning brush during the cleaning process. This ensures that the deep hole plate maintains a stable position during the cleaning process, thereby guaranteeing the cleaning effect.

[0021] Furthermore, each cleaning brush includes a connecting block, which is partially placed inside a connecting plate. A spring is fixedly connected to the bottom of each connecting block, and a brush rod is fixedly connected to the other end of each spring. A brush head is fixedly connected to the bottom of each brush rod, and a rotating ring is fitted on each brush rod. The top of the rotating ring is rotatably connected to the bottom of the connecting block, and several bristles are fixedly connected to the outer wall of the rotating ring.

[0022] Beneficial effects: Due to the elasticity of the spring, the brush rod can move up and down within a certain range, allowing the brush head to be adjusted according to the depth of different holes in the deep hole plate, ensuring that the brush head can fully contact the bottom of the hole, improving the comprehensiveness and thoroughness of cleaning; a rotating ring is fitted on the brush rod, and several bristles are fixed on the outer wall of the rotating ring. During the cleaning process, the rotating ring can rotate relative to the brush rod, allowing the bristles to brush the hole wall of the deep hole plate at different angles, effectively removing stains and residues on the hole wall, improving the cleaning effect. Compared with the traditional fixed bristle design, this rotatable bristle can cover a wider cleaning area and reduce cleaning dead corners.

[0023] Furthermore, rotating blocks are fixedly connected to the inner wall of each rotating ring, and spiral annular grooves corresponding to the size of the rotating blocks are provided on each brush rod.

[0024] Beneficial effects: The cooperation between the rotating block and the spiral annular groove provides stable support and guidance for the rotation of the rotating ring on the brush rod. The rotating block can move smoothly within the spiral annular groove, ensuring that the rotating ring can rotate stably around the brush rod, so that the bristles can evenly brush the hole wall of the deep hole plate, improving the consistency and effect of cleaning.

[0025] Furthermore, each brush head is fitted with a sponge layer.

[0026] Beneficial effects: The soft sponge layer acts as a buffer between the brush head and the deep hole plate, reducing direct contact and friction between the brush head and the deep hole plate, and preventing scratches on the surface of the deep hole plate during cleaning. It is especially suitable for deep hole plates made of soft materials or with easily damaged surfaces. At the same time, the sponge layer expands after absorbing the cleaning solution, increasing the contact area between the brush head and the deep hole plate. This allows for a more even distribution of the cleaning solution on the surface of the deep hole, thereby improving the uniformity of cleaning and ensuring that all parts of the deep hole plate are thoroughly cleaned.

[0027] Furthermore, several bases are installed on the bottom of the base plate, and each base has a suction cup on its bottom.

[0028] Beneficial effects: The base acts as an isolation and buffer, preventing the base plate from directly contacting the placement surface and being worn, scratched, or corroded; the suction cup can firmly adhere to a smooth surface, such as a laboratory table or the bottom of a cleaning tank, firmly fixing the entire cleaning device in a specific position, preventing it from moving or shaking during the cleaning process due to vibration, water flow impact, or accidental collision, and ensuring the stability and accuracy of the cleaning work.

[0029] Furthermore, the bristles are all in contact with the inner wall of the holes in the deep hole plate.

[0030] Beneficial effects: The bristles fit tightly against the walls of the deep-hole plate, generating greater friction during cleaning and more effectively removing stains and impurities adhering to the hole walls, thus improving the cleanliness of the cleaning process.

[0031] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0032] Figure 1 This is a side view of an embodiment of the cleaning device for deep-well plates for laboratory DNA extraction according to this utility model;

[0033] Figure 2 This is a front view of an embodiment of the cleaning device for deep-well plates for laboratory DNA extraction according to this utility model;

[0034] Figure 3 This is a structural diagram of the cleaning brush in an embodiment of the cleaning device for deep-well plates used in laboratory DNA extraction according to this utility model.

[0035] The reference numerals in the accompanying drawings of the instruction manual include: 1. Base plate; 2. Support plate; 3. Top plate; 4. Outer shell; 5. Electric cylinder; 6. Connecting plate; 7. Cleaning brush; 8. Deep hole plate; 9. Fixing block; 10. Placement groove; 11. Base; 12. Suction cup; 71. Connecting block; 72. Spring; 73. Brush rod; 74. Spiral annular groove; 75. Rotating block; 76. Rotating ring; 77. Brush bristles; 78. Brush head. Detailed Implementation

[0036] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0037] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0038] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0039] The following detailed description illustrates the specific implementation method:

[0040] Example 1:

[0041] As attached Figure 1 As shown: A cleaning device for deep-well plates used in laboratory DNA extraction includes a base plate 1. A support plate 2 is fixedly connected to one side of the base plate 1 by tenon and mortise. A top plate 3 is fixedly connected to the top of the support plate 2 by tenon and mortise. The top plate 3, support plate 2, and base plate 1 form a support frame, which serves to support and fix the plate. A lifting component is detachably connected to the bottom of the top plate 3 by a snap fastener. A connecting plate 6 is fixedly connected to the bottom of the lifting component. Several cleaning brushes 7 are detachably connected to the bottom of the connecting plate 6 by snap fasteners. The lifting component controls the lifting and lowering of the connecting plate 6, thereby driving the cleaning brushes 7 to move up and down, thereby performing up and down friction cleaning on the inner wall of the deep-well plate 8. The detachable setting of the cleaning brushes 7 can meet the cleaning needs of deep-well plates 8 with different numbers of wells, such as 48 wells and 12 wells.

[0042] A deep hole plate 8 is detachably connected to the top of the base plate 1. The top of the base plate 1 is provided with a placement groove 10 corresponding to the size of the bottom of the deep hole plate 8, which facilitates the placement or removal of the deep hole plate 8. At the same time, the position setting of the placement groove 10 can ensure that the deep hole plate 8 is placed in the same position each time. During the cleaning process of the deep hole plate 8, the deep hole plate 8 may shake, which may affect its cleaning effect or even cause damage to the deep hole plate 8. Therefore, fixing blocks 9 are hinged on both sides of the placement groove 10. Torsion springs are provided at the hinge points between the fixing blocks 9 and the base plate 1. The fixing blocks 9 can strengthen the fixation of the deep hole plate 8.

[0043] The lifting assembly includes a housing 4, inside which an electric control cylinder 5 is installed. The preferred model of the electric control cylinder 5 is FestoEGC-MF series. The output shaft of the electric control cylinder 5 extends towards the base plate 1 to the outside of the housing 4 and is fixedly connected to the top of the connecting plate 6 by adhesive bonding. That is, by controlling the extension and retraction of the output shaft of the electric control cylinder 5, the lifting and lowering of the cleaning brush 7 can be controlled, which can perform automated cleaning and reduce the workload of the staff.

[0044] Meanwhile, the number and position of the cleaning brushes 7 correspond to the number and position of the deep hole plates 8, ensuring that each hole has a corresponding cleaning brush 7 for cleaning, and the cleaning of an entire deep hole plate 8 can be completed in one go, greatly improving work efficiency.

[0045] The specific implementation process is as follows: When cleaning the deep hole plate 8, if the operator chooses manual cleaning, one hand holds the deep hole plate 8 filled with cleaning fluid, and the other hand holds the outer casing 4, i.e., holding the electric control cylinder 5, connecting plate 6, and cleaning brush 7 fixedly connected to it. After aligning the cleaning brush 7 with the corresponding hole on the deep hole plate 8, the operator moves the outer casing 4 up and down, which drives the cleaning brush 7 to move in and out of the hole, thereby cleaning the inner wall of the hole by rubbing it up and down. If no operator is holding the cleaning brush, the deep hole plate 8 can be placed in the placement slot 10 on the base 11, and the fixing block 9 can be twisted to fix the deep hole plate 8. The outer casing 4 is installed at the bottom of the top plate 3, i.e., the electric control cylinder 5, connecting plate 6, and cleaning brush 7 are installed. At the same time, the position of the cleaning brush 7 corresponds to the corresponding deep hole. At this time, the operator only needs to open the electric control cylinder 5 to control the extension and retraction of its output shaft, i.e., control the cleaning brush 7 to move in and out of the hole, and the cleaning brush 7 rubs the inner wall of the hole to clean the deep hole plate 8. The entire deep hole plate 8 can be cleaned in one go, and the operator can operate quickly with one hand, which greatly reduces the workload.

[0046] Example 2:

[0047] As attached Figure 1As shown, the difference from Embodiment 1 is that each cleaning brush 7 includes a connecting block 71, which is partially placed inside the connecting plate 6. The connecting block 71 is snapped onto the connecting plate 6 for easy disassembly and installation. When the cleaning brush 7 only rubs up and down against the inner wall of the hole, there may be friction dead angles, resulting in incomplete cleaning of the deep hole plate 8. Therefore, a spring 72 is fixedly connected to the bottom of each connecting block 71 by welding. A brush rod 73 is fixedly connected to the other end of each spring 72. A brush head 78 is fixedly connected to the bottom of each brush rod 73 by a snap-fit. A rotating ring 76 is fitted on each brush rod 73. The top of the rotating ring 76 is rotatably connected to the bottom of the connecting block 71. A rotating block 75 is fixedly connected to the inner wall of each rotating ring 76. Each of the 73 components is provided with a spiral annular groove 74 corresponding to the size of the rotating block 75. Several bristles 77 are fixedly connected to the outer wall of the rotating ring 76. Through the above arrangement, the rotating ring 76 can rotate, which drives the brush to rotate. The bristles 77 can rub the inner wall of the hole from top to bottom and in all directions, thus cleaning the deep hole plate 8 more thoroughly. If the brush head 78 is too hard, it may damage the bottom of the hole. Therefore, each brush head 78 is covered with a sponge layer. The sponge layer can absorb water and expand, increasing its contact area with the inner wall of the hole. At the same time, the bristles 77 are all in close contact with the inner wall of the hole of the deep hole plate 8. The bristles 77 are in close contact with the hole wall of the deep hole plate 8, which can generate greater friction during cleaning and clean the deep hole plate 8 more thoroughly.

[0048] The specific implementation process is as follows: When cleaning the deep hole plate 8, as the cleaning brush 7 continues to move downward, the brush head 78 stops moving downward after contacting the bottom wall of the hole. At the same time, the brush rod 73 stops moving and compresses the spring 72. When the external force disappears, the spring 72 will return to its original shape, driving the brush rod 73 to move up and down, thereby causing the brush head 78 to move up and down, achieving contact and separation from the inner wall of the hole. Meanwhile, the sponge layer provides a certain elastic buffer during the cleaning process to better conform to the surface of the object for cleaning. When the brush rod 73 moves up and down, the rotating ring 76 cooperates with the spiral annular groove 74 on the brush rod 73 through the rotating block 75 on the inner wall, so that the rotating ring 76 can rotate around the brush rod 73. The bristles 77 fixedly connected to the outer wall of the rotating ring 76 will change direction and position as the rotating ring 76 rotates, thereby enabling a more comprehensive and multi-angle cleaning of the inner wall of the hole, increasing the cleaning coverage and effect. The rotation of the rotating ring 76 is relatively independent of the up and down movement of the brush head 78. The two can be carried out simultaneously without interfering with each other, jointly completing the cleaning action of the inner wall of the hole of the deep hole plate 8.

[0049] Example 3:

[0050] As attached Figure 1 As shown, the difference from Embodiment 2 is that the base plate 1 may be worn, scratched or corroded when it is in direct contact with the placement surface. Therefore, a number of bases 11 are installed at the bottom of the base plate 1, and each base 11 is provided with a suction cup 12 to facilitate the fixing of the device and prevent the device from moving during the cleaning of the deep hole plate 8.

[0051] The specific implementation process is as follows: When the deep hole plate 8 needs to be cleaned, the cleaning device is taken out. The suction cup 12 at the bottom of the base plate 1 can be firmly attached to a smooth surface, such as the bottom of the experimental table or the cleaning tank, so that the entire cleaning device is firmly fixed in a specific position to prevent it from moving or shaking due to vibration, water flow impact or accidental collision during the cleaning process, and to ensure the stability and accuracy of the cleaning work.

[0052] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the protection scope of this invention.

Claims

1. A cleaning device for deep-well plates used in laboratory DNA extraction, comprising a base plate (1), a support plate (2) fixedly connected to one side of the base plate (1), and a top plate (3) fixedly connected to the top of the support plate (2), characterized in that, The top plate (3) is detachably connected to a lifting component at the bottom. The bottom of the lifting component is fixedly connected to a connecting plate (6). The bottom of the connecting plate (6) is detachably connected to several cleaning brushes (7). The bottom plate (1) has a detachable deep hole plate (8) at the top.

2. The cleaning device for deep-well plates used in laboratory DNA extraction according to claim 1, characterized in that, The lifting assembly includes a housing (4), an electric cylinder (5) is installed inside the housing (4), and the output shaft of the electric cylinder (5) extends to the outside of the housing (4) in the direction of the base plate (1) and is fixedly connected to the top of the connecting plate (6).

3. The cleaning device for deep-well plates used in laboratory DNA extraction according to claim 2, characterized in that, The number and position of the cleaning brushes (7) correspond to the number and position of the deep hole plates (8), respectively.

4. The cleaning device for deep-well plates used in laboratory DNA extraction according to claim 3, characterized in that, The top of the base plate (1) is provided with a placement groove (10) that corresponds to the size of the bottom of the deep hole plate (8).

5. The cleaning device for deep-well plates used in laboratory DNA extraction according to claim 4, characterized in that, Both sides of the placement slot (10) are hinged with fixing blocks (9), and torsion springs are provided at the hinge points between the fixing blocks (9) and the base plate (1).

6. The cleaning apparatus for deep-well plates used in laboratory DNA extraction according to claim 5, characterized in that, Each cleaning brush (7) includes a connecting block (71), which is partially placed inside the connecting plate (6). A spring (72) is fixedly connected to the bottom of each connecting block (71), and a brush rod (73) is fixedly connected to the other end of each spring (72). A brush head (78) is fixedly connected to the bottom of each brush rod (73). A rotating ring (76) is fitted on each brush rod (73). The top of the rotating ring (76) is rotatably connected to the bottom of the connecting block (71), and several bristles (77) are fixedly connected to the outer wall of the rotating ring (76).

7. The cleaning apparatus for deep-well plates used in laboratory DNA extraction according to claim 6, characterized in that, Rotating blocks (75) are fixedly connected to the inner wall of the rotating ring (76), and spiral annular grooves (74) corresponding to the size of the rotating blocks (75) are provided on the brush rod (73).

8. The cleaning apparatus for deep-well plates used in laboratory DNA extraction according to claim 7, characterized in that, Each brush head (78) is covered with a sponge layer.

9. The cleaning apparatus for deep-well plates used in laboratory DNA extraction according to claim 8, characterized in that, The bottom of the base plate (1) is equipped with several bases (11), and each base (11) has a suction cup (12) at its bottom.

10. The cleaning apparatus for deep-well plates used in laboratory DNA extraction according to claim 9, characterized in that, The bristles (77) are all in contact with the inner wall of the hole of the deep hole plate (8).

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